McClain W, Schneider J, Bhattacharya S, Gabriel K. The importance of tRNA backbone-mediated interactions with synthetase for aminoacylation. Proc Natl Acad Sci U S A. 1998;95:460-5 pubmed
..Recognition also includes synthetase interaction with tRNA backbone functionalities whose distinctive locations in three-dimensional space are exquisitely determined by the tRNA sequence. ..
McClain W, Gabriel K. Construction of an Escherichia coli knockout strain for functional analysis of tRNA(Asp). J Mol Biol. 2001;310:537-42 pubmed
..We have developed a system to overcome this deficiency by engineering an E. coli knockout tRNA(Asp) strain, thereby allowing a penetrating analysis of tRNA(Asp) structure and function under conditions that prevail in the cell. ..
McClain W, Jou Y, Bhattacharya S, Gabriel K, Schneider J. The reliability of in vivo structure-function analysis of tRNA aminoacylation. J Mol Biol. 1999;290:391-409 pubmed
..These experiments support the authenticity of the cellular assay and imply that a condition or factor present in the cell assay may be absent in the test tube assay. ..