W H McClain


Affiliation: University of Wisconsin
Country: USA


  1. McClain W, Schneider J, Bhattacharya S, Gabriel K. The importance of tRNA backbone-mediated interactions with synthetase for aminoacylation. Proc Natl Acad Sci U S A. 1998;95:460-5 pubmed
    ..Recognition also includes synthetase interaction with tRNA backbone functionalities whose distinctive locations in three-dimensional space are exquisitely determined by the tRNA sequence. ..
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    McClain W, Gabriel K. Construction of an Escherichia coli knockout strain for functional analysis of tRNA(Asp). J Mol Biol. 2001;310:537-42 pubmed
    ..We have developed a system to overcome this deficiency by engineering an E. coli knockout tRNA(Asp) strain, thereby allowing a penetrating analysis of tRNA(Asp) structure and function under conditions that prevail in the cell. ..
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    McClain W, Jou Y, Bhattacharya S, Gabriel K, Schneider J. The reliability of in vivo structure-function analysis of tRNA aminoacylation. J Mol Biol. 1999;290:391-409 pubmed
    ..These experiments support the authenticity of the cellular assay and imply that a condition or factor present in the cell assay may be absent in the test tube assay. ..