BRUCE MAYER

Summary

Affiliation: University of Connecticut Health Center
Country: USA

Publications

  1. ncbi request reprint A single-chain antibody/epitope system for functional analysis of protein-protein interactions
    Kosaku Fujiwara
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 3301, USA
    Biochemistry 41:12729-38. 2002
  2. pmc Stoichiometry of Nck-dependent actin polymerization in living cells
    Jonathon A Ditlev
    Department of Genetics and Developmental Biology, Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, and Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Biol 197:643-58. 2012
  3. pmc Characterizing tyrosine phosphorylation signaling in lung cancer using SH2 profiling
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut, United States of America
    PLoS ONE 5:e13470. 2010
  4. pmc Proteins that bind the Src homology 3 domain of CrkI have distinct roles in Crk transformation
    J Zheng
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 6403, USA
    Oncogene 29:6378-89. 2010
  5. pmc Perspective: Dynamics of receptor tyrosine kinase signaling complexes
    Bruce J Mayer
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    FEBS Lett 586:2575-9. 2012
  6. pmc Phosphorylation of p130Cas initiates Rac activation and membrane ruffling
    Alok Sharma
    Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 1260 Elm Street, Manchester, NH 03101, USA
    BMC Cell Biol 9:50. 2008
  7. pmc Molecular machines or pleiomorphic ensembles: signaling complexes revisited
    Bruce J Mayer
    Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    J Biol 8:81. 2009
  8. pmc Clues to the evolution of complex signaling machinery
    Bruce J Mayer
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 105:9453-4. 2008
  9. ncbi request reprint SH3 domains: complexity in moderation
    B J Mayer
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 3301, USA
    J Cell Sci 114:1253-63. 2001
  10. doi request reprint Detection of protein-protein interactions by far-western blotting
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, 06030, USA
    Methods Mol Biol 536:313-29. 2009

Collaborators

Detail Information

Publications23

  1. ncbi request reprint A single-chain antibody/epitope system for functional analysis of protein-protein interactions
    Kosaku Fujiwara
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 3301, USA
    Biochemistry 41:12729-38. 2002
    ..We expect that this approach, which we term a functional interaction trap, will be a powerful proteomic tool for investigating protein-protein interactions...
  2. pmc Stoichiometry of Nck-dependent actin polymerization in living cells
    Jonathon A Ditlev
    Department of Genetics and Developmental Biology, Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, and Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Biol 197:643-58. 2012
    ..These results provide new insight into activities involving localized actin polymerization, including tumor cell invasion, microbial pathogenesis, and T cell activation...
  3. pmc Characterizing tyrosine phosphorylation signaling in lung cancer using SH2 profiling
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut, United States of America
    PLoS ONE 5:e13470. 2010
    ..However, the comprehensive analysis of tyrosine phosphorylation of large numbers of human cancer specimens is technically challenging using current methods...
  4. pmc Proteins that bind the Src homology 3 domain of CrkI have distinct roles in Crk transformation
    J Zheng
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 6403, USA
    Oncogene 29:6378-89. 2010
    ..These results show that SOS1 is essential for CrkI-induced fibroblast transformation, and also reveal a surprising negative role for Abl kinases in Crk transformation...
  5. pmc Perspective: Dynamics of receptor tyrosine kinase signaling complexes
    Bruce J Mayer
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    FEBS Lett 586:2575-9. 2012
    ..Using the example of receptor tyrosine kinases, this perspective takes a fresh look at the dynamics of the system and their potential impact on signal processing...
  6. pmc Phosphorylation of p130Cas initiates Rac activation and membrane ruffling
    Alok Sharma
    Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 1260 Elm Street, Manchester, NH 03101, USA
    BMC Cell Biol 9:50. 2008
    ..We have also used this approach to address a controversy regarding whether it is Src family kinases or focal adhesion kinase (FAK) that phosphorylates p130Cas in the trimolecular Src-FAK-p130Cas complex...
  7. pmc Molecular machines or pleiomorphic ensembles: signaling complexes revisited
    Bruce J Mayer
    Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    J Biol 8:81. 2009
    ..Conventional mechanical descriptions misrepresent this reality and can be actively counterproductive by misdirecting us away from investigating critical issues...
  8. pmc Clues to the evolution of complex signaling machinery
    Bruce J Mayer
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 105:9453-4. 2008
  9. ncbi request reprint SH3 domains: complexity in moderation
    B J Mayer
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 3301, USA
    J Cell Sci 114:1253-63. 2001
    ..Processes regulated by SH3 domains also raise important questions about the nature of specificity and the overall logic governing networks of protein interactions...
  10. doi request reprint Detection of protein-protein interactions by far-western blotting
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, 06030, USA
    Methods Mol Biol 536:313-29. 2009
    ..We here describe a rapid and simple protocol for far-western blotting, in which GST-tagged Src homology 2 (SH2) domains are used to probe cellular proteins in a phosphorylation-dependent manner...
  11. ncbi request reprint The SH2 domain: versatile signaling module and pharmaceutical target
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    Biochim Biophys Acta 1747:1-25. 2005
    ..These studies have also led to new insights into molecular recognition by the SH2 domain...
  12. ncbi request reprint Profiling the global tyrosine phosphorylation state
    Kazuya Machida
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3301, USA
    Mol Cell Proteomics 2:215-33. 2003
    ..We also describe ongoing efforts to modify and improve SH2 profiling, including the development of a multiplexed assay system that will allow high-throughput functional profiling of the tyrosine phosphoproteome...
  13. ncbi request reprint Protein-protein interactions in signaling cascades
    Bruce J Mayer
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, USA
    Methods Mol Biol 332:79-99. 2006
    ..In this chapter, a variety of protein interaction modules that participate in signaling are discussed, and issues relating to binding specificity and the significance of a particular interaction are considered...
  14. pmc Distinct roles for Crk adaptor isoforms in actin reorganization induced by extracellular signals
    Susumu Antoku
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 3301, USA
    J Cell Sci 122:4228-38. 2009
    ....
  15. ncbi request reprint High-throughput phosphotyrosine profiling using SH2 domains
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Cell 26:899-915. 2007
    ..These results demonstrate that high-throughput comprehensive SH2 profiling provides valuable mechanistic insights into tyrosine kinase signaling pathways...
  16. pmc A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors
    Susumu Antoku
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 3301 USA
    J Cell Sci 121:3071-82. 2008
    ..These results uncover a previously unappreciated role for Abl PxxP motifs in the regulation of cell spreading...
  17. pmc A reciprocal interdependence between Nck and PI(4,5)P(2) promotes localized N-WASp-mediated actin polymerization in living cells
    Gonzalo M Rivera
    Raymond and Beverly Sacker Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology and Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Cell 36:525-35. 2009
    ..This study uncovers a strong reciprocal interdependence between Nck and PI(4,5)P(2) in promoting localized N-WASp-mediated actin polymerization in cells...
  18. ncbi request reprint Functional interaction trap: a strategy for validating the functional consequences of tyrosine phosphorylation of specific substrates in vivo
    Alok Sharma
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    Mol Cell Proteomics 2:1217-24. 2003
    ..This strategy provides a powerful tool for validating the functional significance of tyrosine phosphorylation and other post-translational modifications identified by proteomic discovery efforts...
  19. ncbi request reprint Inducible clustering of membrane-targeted SH3 domains of the adaptor protein Nck triggers localized actin polymerization
    Gonzalo M Rivera
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    Curr Biol 14:11-22. 2004
    ..Although several studies have implicated Nck in regulating actin polymerization, its role in living cells is not well understood...
  20. ncbi request reprint A FRET-based probe for epidermal growth factor receptor bound non-covalently to a pair of synthetic amphipathic helixes
    Reina E Itoh
    Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    Exp Cell Res 307:142-52. 2005
    ....
  21. ncbi request reprint Quantitative multiplexed profiling of cellular signaling networks using phosphotyrosine-specific DNA-tagged SH2 domains
    Kevin Dierck
    Department of Clinical Chemistry, Center of Clinical Pathology, University Medical Center Hamburg Eppendorf, Martinistr 52, 20246 Hamburg, Germany
    Nat Methods 3:737-44. 2006
    ....
  22. ncbi request reprint Normalization of nomenclature for peptide motifs as ligands of modular protein domains
    Rein Aasland
    Department of Molecular Biology, University of Bergen, 5020 Bergen, Norway
    FEBS Lett 513:141-4. 2002
    ..This proposal will be reviewed in the future and will therefore be open for the inclusion of new rules, modifications and changes...

Research Grants14

  1. Functional Role of Nck and Crk Binding Interactions
    Bruce J Mayer; Fiscal Year: 2010
    ..The proposed studies will provide specific, mechanistic details about adaptor function that will provide insight into basic biological processes as well as how these processes can be subverted in disease. ..
  2. Functional Role of Nck and Crk Binding Interactions
    BRUCE MAYER; Fiscal Year: 2009
    ..The proposed studies will provide specific, mechanistic details about adaptor function that will provide insight into basic biological processes as well as how these processes can be subverted in disease. ..
  3. Functional Role of Nck and Crk Binding Interactions
    BRUCE MAYER; Fiscal Year: 2007
    ..abstract_text> ..
  4. Functional Role of Nck and Crk Binding Interactions
    BRUCE MAYER; Fiscal Year: 2006
    ..abstract_text> ..
  5. Profiling Tumor Cells by SH2 Domain Binding Pattern
    BRUCE MAYER; Fiscal Year: 2002
    ..Variants of the existing profiling method will also be pursued, with the aim of developing a high-throughput method to quantitate binding sites for tens to hundreds of different SH2 domains in a single binding reaction. ..
  6. Functional Role of Nck and Crk Binding Interactions
    BRUCE MAYER; Fiscal Year: 2004
    ..abstract_text> ..
  7. A high- multiplexed phosphotyrosine profiling assay
    BRUCE MAYER; Fiscal Year: 2007
    ..They will also establish the feasibility of the OTM approach as a more general proteomic tool for the rapid and sensitive profiling of clinical samples. ..
  8. Functional Role of Nck and Crk Binding Interactions
    BRUCE MAYER; Fiscal Year: 2005
    ..abstract_text> ..