Research Topics
| BRUCE MAYERSummaryAffiliation: University of Connecticut Health Center Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
A single-chain antibody/epitope system for functional analysis of protein-protein interactionsKosaku Fujiwara
Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 3301, USA
Biochemistry 41:12729-38. 2002..We expect that this approach, which we term a functional interaction trap, will be a powerful proteomic tool for investigating protein-protein interactions...
Perspective: Dynamics of receptor tyrosine kinase signaling complexesBruce J Mayer
Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
FEBS Lett 586:2575-9. 2012..Using the example of receptor tyrosine kinases, this perspective takes a fresh look at the dynamics of the system and their potential impact on signal processing...- Phosphorylation of p130Cas initiates Rac activation and membrane rufflingAlok Sharma
Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 1260 Elm Street, Manchester, NH 03101, USA
BMC Cell Biol 9:50. 2008..We have also used this approach to address a controversy regarding whether it is Src family kinases or focal adhesion kinase (FAK) that phosphorylates p130Cas in the trimolecular Src-FAK-p130Cas complex... - Molecular machines or pleiomorphic ensembles: signaling complexes revisitedBruce J Mayer
Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
J Biol 8:81. 2009..Conventional mechanical descriptions misrepresent this reality and can be actively counterproductive by misdirecting us away from investigating critical issues... - Clues to the evolution of complex signaling machineryBruce J Mayer
Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
Proc Natl Acad Sci U S A 105:9453-4. 2008 - SH3 domains: complexity in moderationB J Mayer
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 3301, USA
J Cell Sci 114:1253-63. 2001..Processes regulated by SH3 domains also raise important questions about the nature of specificity and the overall logic governing networks of protein interactions... 
Detection of protein-protein interactions by far-western blottingKazuya Machida
Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, 06030, USA
Methods Mol Biol 536:313-29. 2009..We here describe a rapid and simple protocol for far-western blotting, in which GST-tagged Src homology 2 (SH2) domains are used to probe cellular proteins in a phosphorylation-dependent manner...- The SH2 domain: versatile signaling module and pharmaceutical targetKazuya Machida
Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
Biochim Biophys Acta 1747:1-25. 2005..These studies have also led to new insights into molecular recognition by the SH2 domain... - Protein-protein interactions in signaling cascadesBruce J Mayer
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, USA
Methods Mol Biol 332:79-99. 2006..In this chapter, a variety of protein interaction modules that participate in signaling are discussed, and issues relating to binding specificity and the significance of a particular interaction are considered... - Profiling the global tyrosine phosphorylation stateKazuya Machida
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030-3301, USA
Mol Cell Proteomics 2:215-33. 2003..We also describe ongoing efforts to modify and improve SH2 profiling, including the development of a multiplexed assay system that will allow high-throughput functional profiling of the tyrosine phosphoproteome... - Distinct roles for Crk adaptor isoforms in actin reorganization induced by extracellular signalsSusumu Antoku
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 3301, USA
J Cell Sci 122:4228-38. 2009.... - High-throughput phosphotyrosine profiling using SH2 domainsKazuya Machida
Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
Mol Cell 26:899-915. 2007..These results demonstrate that high-throughput comprehensive SH2 profiling provides valuable mechanistic insights into tyrosine kinase signaling pathways... - Functional interaction trap: a strategy for validating the functional consequences of tyrosine phosphorylation of specific substrates in vivoAlok Sharma
Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-3301, USA
Mol Cell Proteomics 2:1217-24. 2003..This strategy provides a powerful tool for validating the functional significance of tyrosine phosphorylation and other post-translational modifications identified by proteomic discovery efforts... - A reciprocal interdependence between Nck and PI(4,5)P(2) promotes localized N-WASp-mediated actin polymerization in living cellsGonzalo M Rivera
Raymond and Beverly Sacker Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology and Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
Mol Cell 36:525-35. 2009..This study uncovers a strong reciprocal interdependence between Nck and PI(4,5)P(2) in promoting localized N-WASp-mediated actin polymerization in cells... - A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptorsSusumu Antoku
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030 3301 USA
J Cell Sci 121:3071-82. 2008..These results uncover a previously unappreciated role for Abl PxxP motifs in the regulation of cell spreading... - Inducible clustering of membrane-targeted SH3 domains of the adaptor protein Nck triggers localized actin polymerizationGonzalo M Rivera
Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
Curr Biol 14:11-22. 2004.... - Normalization of nomenclature for peptide motifs as ligands of modular protein domainsRein Aasland
Department of Molecular Biology, University of Bergen, 5020 Bergen, Norway
FEBS Lett 513:141-4. 2002..This proposal will be reviewed in the future and will therefore be open for the inclusion of new rules, modifications and changes... - A FRET-based probe for epidermal growth factor receptor bound non-covalently to a pair of synthetic amphipathic helixesReina E Itoh
Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita-shi, Osaka 565-0871, Japan
Exp Cell Res 307:142-52. 2005.... - Quantitative multiplexed profiling of cellular signaling networks using phosphotyrosine-specific DNA-tagged SH2 domainsKevin Dierck
Department of Clinical Chemistry, Center of Clinical Pathology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany
Nat Methods 3:737-44. 2006....
Research Grants
- Functional Role of Nck and Crk Binding InteractionsBruce J Mayer; Fiscal Year: 2010..The proposed studies will provide specific, mechanistic details about adaptor function that will provide insight into basic biological processes as well as how these processes can be subverted in disease. ..
- Functional Role of Nck and Crk Binding InteractionsBRUCE MAYER; Fiscal Year: 2009..The proposed studies will provide specific, mechanistic details about adaptor function that will provide insight into basic biological processes as well as how these processes can be subverted in disease. ..
- A high- multiplexed phosphotyrosine profiling assayBRUCE MAYER; Fiscal Year: 2007..They will also establish the feasibility of the OTM approach as a more general proteomic tool for the rapid and sensitive profiling of clinical samples. ..
- Functional Role of Nck and Crk Binding InteractionsBRUCE MAYER; Fiscal Year: 2007..abstract_text> ..
- Profiling Tumor Cells by SH2 Domain Binding PatternBRUCE MAYER; Fiscal Year: 2002..Variants of the existing profiling method will also be pursued, with the aim of developing a high-throughput method to quantitate binding sites for tens to hundreds of different SH2 domains in a single binding reaction. ..