P Masson

Summary

Affiliation: University of Nebraska Medical Center
Country: USA

Publications

  1. ncbi Pesticides and susceptible populations: people with butyrylcholinesterase genetic variants may be at risk
    O Lockridge
    University of Nebraska Medical Center, Eppley Institute, Omaha 68198 6805, USA
    Neurotoxicology 21:113-26. 2000
  2. ncbi Rat butyrylcholinesterase-catalysed hydrolysis of N-alkyl homologues of benzoylcholine
    Anna Hrabovska
    Comenius University, Faculty of Pharmacy, Department of Cell and Molecular Biology of Drugs, Bratislava, Slovakia
    FEBS J 273:1185-97. 2006
  3. pmc Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry assay for organophosphorus toxicants bound to human albumin at Tyr411
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA
    Anal Biochem 361:263-72. 2007
  4. doi Effects of viscosity and osmotic stress on the reaction of human butyrylcholinesterase with cresyl saligenin phosphate, a toxicant related to aerotoxic syndrome: kinetic and molecular dynamics studies
    Patrick Masson
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 5950, USA
    Biochem J 454:387-99. 2013
  5. doi Time-dependent kinetic complexities in cholinesterase-catalyzed reactions
    P Masson
    Institut de Recherches Biomédicales des Armées CRSSA, La Tronche, Cedex 38702, France
    Biochemistry (Mosc) 77:1147-61. 2012
  6. pmc Human-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication
    Thomas Cherrier
    Institut de Parasitologie et Pathologie Tropicale, EA 4438, Universite de Strasbourg, 3 Rue Koeberle, 67000 Strasbourg, France
    Virol J 8:352. 2011
  7. ncbi [Presentation. Enzymes: the long pathway from genes to drugs]
    P Masson
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, BP 87, F 38702 La Tronche Cedex
    Ann Pharm Fr 65:95-7. 2007
  8. pmc Butyrylcholinesterase for protection from organophosphorus poisons: catalytic complexities and hysteretic behavior
    Patrick Masson
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 5950, USA
    Arch Biochem Biophys 494:107-20. 2010
  9. doi Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters
    Patrick Masson
    Unite d Enzymologie, Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, La Tronche Cedex, France
    FEBS J 275:2617-31. 2008
  10. ncbi [Engineering of catalytic bioscavengers of organophosphorus compounds]
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, BP 87, 38702 La Tronche
    Bull Acad Natl Med 191:95-111; discussion 112. 2007

Collaborators

Detail Information

Publications101 found, 100 shown here

  1. ncbi Pesticides and susceptible populations: people with butyrylcholinesterase genetic variants may be at risk
    O Lockridge
    University of Nebraska Medical Center, Eppley Institute, Omaha 68198 6805, USA
    Neurotoxicology 21:113-26. 2000
    ..More studies are needed to strengthen the hypothesis that people with genetic variants of BChE are at higher risk of intoxication from pesticides...
  2. ncbi Rat butyrylcholinesterase-catalysed hydrolysis of N-alkyl homologues of benzoylcholine
    Anna Hrabovska
    Comenius University, Faculty of Pharmacy, Department of Cell and Molecular Biology of Drugs, Bratislava, Slovakia
    FEBS J 273:1185-97. 2006
    ..substrate concentration showed a premature plateau far below V(max). This was due to the loss of substrate availability. The best substrates for rat butyrylcholinesterase were short alkyl homologues, BzCh - BCH4...
  3. pmc Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry assay for organophosphorus toxicants bound to human albumin at Tyr411
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA
    Anal Biochem 361:263-72. 2007
    ..Our results show that OP pesticides and nerve agents bind covalently to human albumin at Tyr411. The presence of Tyr411 on an exposed surface of albumin suggests that an antibody response could be generated against OP-albumin adducts...
  4. doi Effects of viscosity and osmotic stress on the reaction of human butyrylcholinesterase with cresyl saligenin phosphate, a toxicant related to aerotoxic syndrome: kinetic and molecular dynamics studies
    Patrick Masson
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 5950, USA
    Biochem J 454:387-99. 2013
    ..MD simulations showed that glycerol molecules that substitute for water molecules in the enzyme active-site gorge induce a conformational change in the catalytic triad residue His⁴³⁸, leading to the less reactive form E'...
  5. doi Time-dependent kinetic complexities in cholinesterase-catalyzed reactions
    P Masson
    Institut de Recherches Biomédicales des Armées CRSSA, La Tronche, Cedex 38702, France
    Biochemistry (Mosc) 77:1147-61. 2012
    ..g. cholinergic synaptic clefts. Therefore, physiological or toxicological relevance of the hysteretic behavior and damped oscillations in ChE-catalyzed reactions and inhibition cannot be ruled out...
  6. pmc Human-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication
    Thomas Cherrier
    Institut de Parasitologie et Pathologie Tropicale, EA 4438, Universite de Strasbourg, 3 Rue Koeberle, 67000 Strasbourg, France
    Virol J 8:352. 2011
    ..Our results revealed HPBP as a new and potent anti HIV molecule that inhibits transcription of the virus, which has not yet been targeted by HAART and therefore opens new strategies in the treatment of HIV infection...
  7. ncbi [Presentation. Enzymes: the long pathway from genes to drugs]
    P Masson
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, BP 87, F 38702 La Tronche Cedex
    Ann Pharm Fr 65:95-7. 2007
  8. pmc Butyrylcholinesterase for protection from organophosphorus poisons: catalytic complexities and hysteretic behavior
    Patrick Masson
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 5950, USA
    Arch Biochem Biophys 494:107-20. 2010
    ..It is proposed, but not yet proven, that low dose exposure involves OP modification of proteins that have no active site serine...
  9. doi Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters
    Patrick Masson
    Unite d Enzymologie, Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, La Tronche Cedex, France
    FEBS J 275:2617-31. 2008
    ..The complex inhibition/activation effects of effectors, depending on the integrity of the peripheral anionic site, reflect the allosteric 'cross-talk' between the peripheral anionic site and the catalytic centre...
  10. ncbi [Engineering of catalytic bioscavengers of organophosphorus compounds]
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, BP 87, 38702 La Tronche
    Bull Acad Natl Med 191:95-111; discussion 112. 2007
    ..Protein engineering can yield enzymes with improved catalytic properties, that are stable during storage and in the bloodstream, and that are immunologically compatible. Large-scale production must be feasible and reasonably inexpensive...
  11. ncbi Structure, activities and biomedical applications of human butyrylcholinesterase
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Department of Toxicology, Enzymology Unit, BP 87, 38702 La Tronche Cedex, France
    Protein Pept Lett 16:1215-24. 2009
    ..Also, BuChE has been integrated in biosensors for detection of organophosphorus compounds and other cholinesterase inhibitors...
  12. doi Structural approach to the aging of phosphylated cholinesterases
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Dept Toxicology, 38702 La Tronche Cedex, France
    Chem Biol Interact 187:157-62. 2010
    ..Knowledge of the molecular basis of aging will help to design reactivators of aged ChEs, molecules capable of slowing the aging process, and pseudocatalytic ChE-based bioscavengers...
  13. ncbi Hydrolysis of oxo- and thio-esters by human butyrylcholinesterase
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, BP 87, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1774:16-34. 2007
    ..Interaction of these groups with the ethereal hetero-atom can have a neutral, an additive or an antagonistic effect on transition state stabilization, depending on their molecular structure, size and enantiomeric configuration...
  14. doi A collaborative endeavor to design cholinesterase-based catalytic scavengers against toxic organophosphorus esters
    Patrick Masson
    Toxicology Department, Enzymology Unit, Centre de Recherches du Service de Sante des Armees, La Tronche Cedex, France
    Chem Biol Interact 175:273-80. 2008
    ..Additional mutations were made for optimizing activity of the new dyad. It is anticipated that these new mutants will have OP hydrolase activity...
  15. ncbi Hysteresis of butyrylcholinesterase in the approach to steady-state kinetics
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, BP 87, 38702 La Tronche Cedex, France
    Chem Biol Interact 157:143-52. 2005
    ..Therefore, a physiological or toxicological relevance for the hysteresis in BChE cannot be ruled out...
  16. doi Evolution of and perspectives on therapeutic approaches to nerve agent poisoning
    Patrick Masson
    IRBA CRSSA, Toxicology Dept, 38702 La Tronche Cedex, France
    Toxicol Lett 206:5-13. 2011
    ....
  17. ncbi Linear and non-linear pressure dependence of enzyme catalytic parameters
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, BP 87, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1724:440-50. 2005
    ..e., temperature, solvent composition, pH), pressure-induced enzyme unfolding, compressibility changes and pressure-induced rate limiting changes. These different situations are illustrated with several examples...
  18. ncbi Identification of residues essential for human paraoxonase (PON1) arylesterase/organophosphatase activities
    D Josse
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, La Tronche, France
    Biochemistry 38:2816-25. 1999
    ..Finally, the cysteines constituting the PON1 disulfide bond (C41 and C352) were essential, but the glycan chains linked to Asn 252 and 323 were not essential for PON1 secretion and activity...
  19. ncbi Structural and hydration changes in the active site gorge of phosporhylated butyrylcholinesterase accompanying the aging process
    P Masson
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, La Tronche, France
    Chem Biol Interact 119:17-27. 1999
    ..In mutant enzymes, mutated residues function like check valves: forced penetration of water in the gorge is difficult, release of water is facilitated...
  20. ncbi Asp7O in the peripheral anionic site of human butyrylcholinesterase
    P Masson
    Centre de Recherches du Service de Sante des Armees, Unite de Biochimie, La Tronche, France
    Eur J Biochem 235:36-48. 1996
    ....
  21. pmc Hydration change during the aging of phosphorylated human butyrylcholinesterase: importance of residues aspartate-70 and glutamate-197 in the water network as probed by hydrostatic and osmotic pressures
    P Masson
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, BP 87, 38702 La Tronche Cedex, France
    Biochem J 343:361-9. 1999
    ..e. forced penetration of water into the gorge is not easily achieved, thereby facilitating the release of water...
  22. ncbi Interaction between the peripheral site residues of human butyrylcholinesterase, D70 and Y332, in binding and hydrolysis of substrates
    P Masson
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, P O Box 87, 38702, La Tronche Cedex, France
    Biochim Biophys Acta 1433:281-93. 1999
    ..It is concluded that double mutations on the PAS induce a conformational change in the active site gorge of butyrylcholinesterase that can alter both substrate binding and enzyme acylation...
  23. pmc Importance of aspartate-70 in organophosphate inhibition, oxime re-activation and aging of human butyrylcholinesterase
    P Masson
    Centre de Recherches du Service de Sante des Armees, Unite de Biochimie, BP 87, 38702 La Tronche Cedex, France and Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Biochem J 325:53-61. 1997
    ..People with the D70G mutation will be resistant to re-activation of their inhibited BuChE by 2-PAM, but this will be offset by the lower rate of irreversible aging of inhibited BuChE, allowing some regeneration by spontaneous hydrolysis...
  24. ncbi Tryptophan residue(s) as major components of the human serum paraoxonase active site
    D Josse
    Eppley Institute, University of Nebraska Medical Center, Omaha 68198 6805, USA
    Chem Biol Interact 119:79-84. 1999
    ..Trp residue(s) may be involved in the binding of aromatic substrates...
  25. ncbi A single amino acid substitution, Gly117His, confers phosphotriesterase (organophosphorus acid anhydride hydrolase) activity on human butyrylcholinesterase
    O Lockridge
    Eppley Institute, University of Nebraska Medical Center, Omaha 68198 6805, USA
    Biochemistry 36:786-95. 1997
    ..Echothiophate and paraoxon were hydrolyzed with the same kcat of 0.75 min-1. This calculates to a rate acceleration of 100,000-fold for hydrolysis of echothiophate and paraoxon by the G117H mutant compared to the nonenzymatic rate...
  26. ncbi Measuring conformational stability of proteins using an optimized temperature-controlled capillary electrophoresis approach
    D Rochu
    Centre de Recherches, Unite d Enzymologie, BP 87, La Tronche, France
    J Chromatogr A 838:157-65. 1999
    ..Accordingly, CZE is a powerful tool to study protein unfolding/folding quickly and with minimal sample requirements...
  27. ncbi Differential effect of pressure and temperature on the catalytic behaviour of wild-type human butyrylcholinesterase and its D70G mutant
    A Weingand-Ziade
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, La Tronche, France
    Eur J Biochem 264:327-35. 1999
    ..The results indicate that the residue D70 controls the conformational plasticity of the active site gorge of BuChE, and is involved in regulation of the catalytic activity as a function of temperature...
  28. ncbi Recombinant human butyrylcholinesterase G390V, the fluoride-2 variant, expressed in Chinese hamster ovary cells, is a low affinity variant
    P Masson
    Centre de Recherches du Service de Sante des Armees, Unite de Biochimie, La Tronche, France
    J Biol Chem 268:14329-41. 1993
    ..Thus, Km and Ki changes explain the succinyldicholine sensitivity of people carrying the fluoride-2 variant...
  29. ncbi Effects of mutations of active site residues and amino acids interacting with the Omega loop on substrate activation of butyrylcholinesterase
    P Masson
    Center de Recherches du Service de Santé des Armées, La Tronche, France
    Biochim Biophys Acta 1544:166-76. 2001
    ..W430 as well as W231 and W82 appear to form the wall of the active site gorge. Mutation of any of these tryptophans disrupts the architecture of the active site...
  30. ncbi Resistance of butyrylcholinesterase to inactivation by ultrasound: effects of ultrasound on catalytic activity and subunit association
    M T Froment
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, BP 87, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1387:53-64. 1998
    ..Aromatic/aromatic interactions between the four helical segments are thought to be enhanced by ultrasound-generated pressure...
  31. ncbi Polyol-induced activation by excess substrate of the D70G butyrylcholinesterase mutant
    V Levitsky
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, La Tronche, France
    Biochim Biophys Acta 1429:422-30. 1999
    ..Osmolyte-induced change in conformation and/or hydration status of Y332 and other solvent-exposed residues may account for the non-Michaelian behaviour of the D70G mutant...
  32. ncbi Human serum paraoxonase (PON1): identification of essential amino acid residues by group-selective labelling and site-directed mutagenesis
    D Josse
    Eppley Institute, University of Nebraska Medical Center, Omaha 68198 6805, USA
    Chem Biol Interact 119:71-8. 1999
    ..However, we cannot rule out that the effects of mutations on catalytic properties resulted from a remote conformational change and/or misfolding of mutant proteins...
  33. ncbi Dual effect of high electric field in capillary electrophoresis study of the conformational stability of Bungarus fasciatus acetylcholinesterase
    D Rochu
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, La Tronche, France
    J Chromatogr A 910:347-57. 2001
    ....
  34. ncbi Kinetic analysis of butyrylcholinesterase-catalyzed hydrolysis of acetanilides
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, BP 87, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1774:1139-47. 2007
    ..It was concluded that the active sites for esterase and AAA activities are identical, i.e. S198. This excludes any other residue present in the gorge for being the catalytic nucleophile pole...
  35. ncbi Rate-determining step of butyrylcholinesterase-catalyzed hydrolysis of benzoylcholine and benzoylthiocholine. Volumetric study of wild-type and D70G mutant behavior
    Patrick Masson
    Centre de Recherches du Service de Santé des Armées CRSSA, Departement de Toxicologie, Unite d Enzymologie, 38702 La Tronche Cedex, France
    Eur J Biochem 271:1980-90. 2004
    ....
  36. ncbi Butyrylcholinesterase-catalyzed hydrolysis of N-methylindoxyl acetate: analysis of volume changes upon reaction and hysteretic behavior
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, BP 87, 24 Av Maquis du Gresivaudan, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1597:229-43. 2002
    ..The E-->E' transition is accompanied by a negative activation volume (DeltaV++(0)= -45+/-10 ml/mol), and the E' form is more compact than E. Hydration water in the gorge of E' appears to be more structured than in the unprimed form...
  37. doi Structure-activity analysis of aging and reactivation of human butyrylcholinesterase inhibited by analogues of tabun
    Eugenie Carletti
    Unite d Enzymologie, Departement de Toxicologie, Centre de Recherches du Service de Santé des Armées CRSSA, 24 av des Maquis du Grésivaudan, 38700 La Tronche, France
    Biochem J 421:97-106. 2009
    ..This study provides the structural basis to design new oximes capable of reactivating phosphoramidyl-hBChE conjugates after intoxication, notably when hBChE is used as pretreatment, or to design BChE-based catalytic bioscavengers...
  38. ncbi Capillary zone electrophoresis with optimized temperature control for studying thermal denaturation of proteins at various pH
    D Rochu
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, La Tronche, France
    Electrophoresis 20:1586-94. 1999
    ..Accordingly, CE can be proposed as a convenient tool to study protein stability and denaturation processes...
  39. ncbi Thermal stability of acetylcholinesterase from Bungarus fasciatus venom as investigated by capillary electrophoresis
    D Rochu
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, La Tronche, France
    Biochim Biophys Acta 1545:216-26. 2001
    ..However, designed mutants of this enzyme capable of degrading organophosphates have to be engineered to enhance their thermostability...
  40. pmc Pressure- and heat-induced inactivation of butyrylcholinesterase: evidence for multiple intermediates and the remnant inactivation process
    A Weingand-Ziade
    , , BP 87, , France
    Biochem J 356:487-93. 2001
    ..A first-order deactivation model describing the kinetics of the remnant inactivation of BuChE is proposed...
  41. ncbi Structural and functional investigations of cholinesterases by means of affinity electrophoresis
    P Masson
    , , La Tronche, France
    Cell Mol Neurobiol 11:173-89. 1991
    ..3. The future prospects of affinity electrophoresis, e.g., investigations on the esteratic site and exploration of the carbohydrate moiety, are emphasized in the concluding section...
  42. ncbi Role of water in aging of human butyrylcholinesterase inhibited by echothiophate: the crystal structure suggests two alternative mechanisms of aging
    Florian Nachon
    The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Biochemistry 44:1154-62. 2005
    ..Pesticides and certain stereoisomers of nerve agents are expected to undergo aging by breaking the P-O bond...
  43. ncbi Damped oscillatory hysteretic behaviour of butyrylcholinesterase with benzoylcholine as substrate
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, La Tronche, France
    Eur J Biochem 271:220-34. 2004
    ..1H-NMR measurements provided evidence for a slow equilibrium between two BzCh conformers. Binding of the conformationally preferred substrate conformer leads to products...
  44. ncbi Evidence for a single butyrylcholinesterase gene in individuals carrying the C5 plasma cholinesterase variant (CHE2)
    P Masson
    Centre de Recherches du Service de Sante des Armees, Unite de Biochimie, La Tronche, France
    FEBS Lett 262:115-8. 1990
    ..This finding supports the suggestion that the C5 variant is a hybrid enzyme resulting from the association of butyrylcholinesterase subunits with a non-cholinesterase protein...
  45. ncbi [Enzyme optimization, mutagenesis and directed evolution]
    T Poyot
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, BP 87, F 38702 La Tronche Cedex
    Ann Pharm Fr 65:119-25. 2007
    ..In our research group, a directed evolution project has been initiated on a bacterial phosphotriesterase, a promising enzyme, capable of efficiently detoxifying organophosphorus nerve agents...
  46. ncbi The active site of human paraoxonase (PON1)
    D Josse
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198-6805, USA
    J Appl Toxicol 21:S7-11. 2001
    ..Fluorescence of PON1 complexed to terbium ion shows that at least one tryptophan is close to the calcium binding site...
  47. doi An unexpected plasma cholinesterase activity rebound after challenge with a high dose of the nerve agent VX
    F Dorandeu
    Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, 24 Avenue des Maquis du Gresivaudan, BP 87, F 38702 La Tronche Cedex, France
    Toxicology 248:151-7. 2008
    ..Nature of the organ(s) and mechanisms involved in enzyme release will need further investigations as it may represent a mechanism of defence, i.e. VX scavenging, that could advantageously be exploited...
  48. ncbi Cold-induced conformational changes of ribonuclease A as investigated by subzero transverse temperature gradient gel electrophoresis
    C Curtil
    Centre de Recherches du Service de Sante des Armees, Unite de Biochimie, La Tronche, France
    Biochim Biophys Acta 1208:1-7. 1994
    ..5 degrees C). We attempted to detect these two transitions by differential scanning calorimetry, UV spectrophotometry and circular dichroism measurements. These transitions have been ascribed to subtle cold-induced conformational changes...
  49. ncbi [Human plasma paraoxonase (HuPON1): an anti-atherogenic enzyme with organophosphate hydrolase activity]
    D Josse
    , , BP87, F38702 La Tronche Cedex, France
    Ann Pharm Fr 59:108-18. 2001
    ..Determination of the PON1 three-dimensional structure is a key step toward improvement of the enzyme functional properties...
  50. ncbi Aging pathways for organophosphate-inhibited human butyrylcholinesterase, including novel pathways for isomalathion, resolved by mass spectrometry
    He Li
    Eppley Institute and Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Toxicol Sci 100:136-45. 2007
    ..e., the classical X-R scission pathway. In contrast, isomalathion aged through both X-R and P-X pathways; the main aged product resulted from P-S bond cleavage and a minor product resulted from O-C and/or S-C bond cleavage...
  51. doi Aging mechanism of butyrylcholinesterase inhibited by an N-methyl analogue of tabun: implications of the trigonal-bipyramidal transition state rearrangement for the phosphylation or reactivation of cholinesterases
    Florian Nachon
    Departement de Toxicologie, Centre de Recherches du Service de Santé des Armées CRSSA, 38702 La Tronche Cedex, France
    Chem Biol Interact 187:44-8. 2010
    ..We suggest that a similar mechanism is also possible for oxime reactivation of phosphylated cholinesterases. This opens new perspectives in terms of reactivator design...
  52. ncbi Aryl acylamidase activity of human serum albumin with o-nitrotrifluoroacetanilide as the substrate
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, BP 87, 38702 La Tronche Cedex, France
    J Enzyme Inhib Med Chem 22:463-9. 2007
    ..6-1 mM), albumin may participate in hydrolysis of aryl acylamides through second-order kinetics. This suggests that albumin may have a role in the metabolism of endogenous and exogenous aromatic amides, including drugs and xenobiotics...
  53. doi Carbofuran poisoning detected by mass spectrometry of butyrylcholinesterase adduct in human serum
    He Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Appl Toxicol 29:149-55. 2009
    ..The method uses 1 ml of serum and detects low-level exposure associated with as little as 20% inhibition of plasma butyrylcholinesterase...
  54. pmc Mass spectrometry identifies covalent binding of soman, sarin, chlorpyrifos oxon, diisopropyl fluorophosphate, and FP-biotin to tyrosines on tubulin: a potential mechanism of long term toxicity by organophosphorus agents
    Hasmik Grigoryan
    Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Chem Biol Interact 175:180-6. 2008
    ..It is concluded that OP bind covalently to tubulin, and that this binding could explain cognitive impairment associated with OP exposure...
  55. doi Aging of cholinesterases phosphylated by tabun proceeds through O-dealkylation
    Eugenie Carletti
    Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, 38700 La Tronche, France
    J Am Chem Soc 130:16011-20. 2008
    ..The reported 3D data will help in the design of new oximes capable of reactivating tabun-ChE conjugates...
  56. pmc Mass spectral characterization of organophosphate-labeled, tyrosine-containing peptides: characteristic mass fragments and a new binding motif for organophosphates
    Lawrence M Schopfer
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 878:1297-311. 2010
    ..Modified peptides are difficult to find when the OP bears no radiolabel and no tag. The characteristic MSMS fragment ions are valuable because they are identifiers for OP-tyrosine, independent of the peptide...
  57. pmc Pseudo-esterase activity of human albumin: slow turnover on tyrosine 411 and stable acetylation of 82 residues including 59 lysines
    Oksana Lockridge
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 283:22582-90. 2008
    ..Acetylated lysines formed adducts that were even more stable. In conclusion, the pseudo-esterase activity of albumin is the result of irreversible acetylation of 82 residues and is not the result of turnover...
  58. doi Mechanism of hydrolysis of dicholine esters with long polymethylene chain by human butyrylcholinesterase
    Hasmik Grigoryan
    Institute of Biotechnology, Gurjyan 14, Yerevan 0056, Armenia
    Biochim Biophys Acta 1784:1818-24. 2008
    ....
  59. ncbi Human paraoxonase: a promising approach for pre-treatment and therapy of organophosphorus poisoning
    Daniel Rochu
    Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, BP 87, 38702 La Tronche Cedex, France
    Toxicology 233:47-59. 2007
    ..coli and natural human PON1 provide information for the future rational design of stable mutants of PON1-based catalytic scavengers to be used as safe and effective countermeasures to OP intoxication...
  60. ncbi Substrate activation in acetylcholinesterase induced by low pH or mutation in the pi-cation subsite
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, La Tronche Cedex, France
    Biochim Biophys Acta 1594:313-24. 2002
    ..The finding that AChE is activated by excess substrate supports the idea that binding of a second substrate molecule to the PAS induces a conformational change that reorganizes the active site...
  61. ncbi [Butyrylcholinesterase: 3D structure, catalytic mechanisms]
    Fl Nachon
    Centre de Recherche du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, BP 87, F38702 La Tronche Cedex
    Ann Pharm Fr 63:194-206. 2005
    ....
  62. ncbi Electrophoresis at elevated hydrostatic pressure of the multiheme hydroxylamine oxidoreductase
    P Masson
    , , La Tronche, France
    Electrophoresis 11:128-33. 1990
    ..At higher pressures, the enzyme was converted to forms which were irreversibly inactive and had a higher apparent molecular mass, suggesting aggregation or denaturation...
  63. ncbi Stabilization of the active form(s) of human paraoxonase by human phosphate-binding protein
    D Rochu
    Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, BP 87, 38702 La Tronche Cedex, France
    Biochem Soc Trans 35:1616-20. 2007
    ..Maintaining a stable environment for such administrable human rPON1 should, at least, preserve the anti-atherogenic activity of the enzyme...
  64. pmc Tyrosines of human and mouse transferrin covalently labeled by organophosphorus agents: a new motif for binding to proteins that have no active site serine
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Toxicol Sci 107:144-55. 2009
    ..The extent to which tyrosine modification by OP can occur in vivo and the toxicological implications of such modifications require further investigation...
  65. ncbi Butyrylcholinesterase, paraoxonase, and albumin esterase, but not carboxylesterase, are present in human plasma
    Bin Li
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Biochem Pharmacol 70:1673-84. 2005
    ..Since human plasma contains no carboxylesterase, only BChE, PON1, and albumin esterases need to be considered when evaluating hydrolysis of an ester drug in human plasma...
  66. ncbi Lamellipodin proline rich peptides associated with native plasma butyrylcholinesterase tetramers
    He Li
    Eppley Institute and Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Biochem J 411:425-32. 2008
    ..We propose that the proline-rich peptides organize the 4 subunits of BChE into a 340 kDa tetramer, by interacting with the C-terminal BChE tetramerization domain...
  67. pmc Five tyrosines and two serines in human albumin are labeled by the organophosphorus agent FP-biotin
    Shi Jian Ding
    Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    Chem Res Toxicol 21:1787-94. 2008
    ..Diethoxyphosphate-labeled Tyr 411 was stable for months at pH 7.4. These results will be useful in the development of specific antibodies to detect OP exposure and to engineer albumin for use as an OP scavenger...
  68. ncbi Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase: expression, purification, characterization and crystallization
    Florian Nachon
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, La Tronche, France
    Eur J Biochem 269:630-7. 2002
    ..73 A3 per Da (estimated 60% solvent) for a single molecule of recombinant BChE in the asymmetric unit. The crystal structure of butyrylcholinesterase will help elucidate unsolved issues concerning cholinesterase mechanisms in general...
  69. pmc Contribution of the active-site metal cation to the catalytic activity and to the conformational stability of phosphotriesterase: temperature- and pH-dependence
    Daniel Rochu
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, BP 87, 38702 La Tronche Cedex, France
    Biochem J 380:627-33. 2004
    ..5-67 degrees C, 58-64 degrees C and 53-64 degrees C respectively). Requirements for optimum activity of PTE (displayed by Co2+-PTE) and maximum stability (displayed by Zn2+-PTE) were demonstrated...
  70. ncbi Stability of highly purified human paraoxonase (PON1): association with human phosphate binding protein (HPBP) is essential for preserving its active conformation(s)
    Daniel Rochu
    Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, BP 87, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1774:874-83. 2007
    ..A hybrid recombinant PON1 was shown more thermostable than the human enzyme, and its stability was unaffected by the presence of HPBP. Altogether, the results strongly encourage further study of the human enzyme...
  71. ncbi Structural data on the aging of diethylphosphoryl-butyrylcholinesterase
    F Nachon
    CRSSA, Department Toxicologie, 38702 La Tronche, France
    Chem Biol Interact 157:408-9. 2005
  72. doi Binding and hydrolysis of soman by human serum albumin
    Bin Li
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Chem Res Toxicol 21:421-31. 2008
    ..Soman-albumin adducts detected by mass spectrometry could be useful for the diagnosis of soman exposure...
  73. ncbi Pressure and heat inactivation of recombinant human acetylcholinesterase. Importance of residue E202 for enzyme stability
    Cécile Cléry-Barraud
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, France
    Eur J Biochem 269:4297-307. 2002
    ..In conclusion, pressure and thermal stability of mutants provided evidence that the residue E202 is a determinant of structural and functional stability of HuAChE...
  74. doi Exploring the structural and functional stabilities of different paraoxonase-1 formulations through electrophoretic mobilities and enzyme activity parameters under hydrostatic pressure
    Cécile Cléry-Barraud
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, BP 87, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1794:680-8. 2009
    ....
  75. ncbi High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine
    Patrick Masson
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, La Tronche, France
    Eur J Biochem 270:315-24. 2003
    ..The mechanism explaining the catalytic behaviour of butyrylcholinesterase at low pH in the presence of excess substrate remains to be elucidated...
  76. ncbi Screening assays for cholinesterases resistant to inhibition by organophosphorus toxicants
    Yuxia Wang
    University of Nebraska Medical Center, Eppley Institute, Omaha, NE 68198 6805, USA
    Anal Biochem 329:131-8. 2004
    ..Both wild-type and G117H BChE were in the epithelial cells of the villi. These assays can be used to identify OP-resistant cholinesterases in culture medium and in animal tissues...
  77. ncbi [Capillary electrophoresis for monitoring stability of pharmaceutical proteins]
    D Rochu
    Centre de Recherches du Service de Sante des Armees, Departement de Toxicologie, Unite d Enzymologie, 24, avenue des Maquis du Grésivaudan, BP 87, F 38702 La Tronche Cedex
    Ann Pharm Fr 61:185-95. 2003
    ..Finally, capillary electrophoresis has proved to be a pertinent tool to validate the conformity of purified enzymes to a status of biopharmaceutical...
  78. ncbi [Action of hydrostatic pressure on proteins: emergence of high pressure biotechnology, potential pharmaceutical and medical applications]
    P Masson
    Centre de Recherches du Service de Santé des Armées Emile Pardé, Departement de Toxicologie, La Tronche
    Ann Pharm Fr 57:49-55. 1999
    ..Moreover, high pressure can be used instead of heart or chemicals for sterilization...
  79. doi Update on biochemical properties of recombinant Pseudomonas diminuta phosphotriesterase
    Eugenie Carletti
    Unite d Enzymologie, Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, 38700 La Tronche, France
    J Enzyme Inhib Med Chem 24:1045-55. 2009
    ..Issues addressed in this work are important for future biotechnological developments of PTE as a detoxifying enzyme...
  80. pmc Detection of adduct on tyrosine 411 of albumin in humans poisoned by dichlorvos
    Bin Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska 68198 5950, USA
    Toxicol Sci 116:23-31. 2010
    ..Identification of other OP-modified proteins may lead to an understanding of neurotoxic symptoms that appear long after the initial OP exposure...
  81. ncbi Oligomeric states of the detergent-solubilized human serum paraoxonase (PON1)
    Denis Josse
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, 24 Avenue des Maquis du Gresivaudan, BP 87, 38702 La Tronche Cedex, France
    J Biol Chem 277:33386-97. 2002
    ..Changes of HDL size and shape could strongly affect the binding affinity and stability of HuPON1 and result in reduced antioxidative capacity of the lipoprotein...
  82. doi Fast affinity purification coupled with mass spectrometry for identifying organophosphate labeled plasma butyrylcholinesterase
    He Li
    Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Chem Biol Interact 175:68-72. 2008
    ..The 29 residue active site tryptic peptide labeled with the nerve agents soman or sarin was identified...
  83. ncbi Multiple advantages of capillary zone electrophoresis for exploring protein conformational stability
    Daniel Rochu
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, La Tronche, France
    Electrophoresis 23:189-202. 2002
    ..Thermal stability, susceptibility to high electric field, alteration of stability by bound ligands and the role of associated cations in metalloenzymes have been successfully investigated...
  84. ncbi Capillary electrophoresis versus differential scanning calorimetry for the analysis of free enzyme versus enzyme-ligand complexes: in the search of the ligand-free status of cholinesterases
    Daniel Rochu
    Centre de Recherches du Service de Sante des Armees, La Tronche, France
    Electrophoresis 27:442-51. 2006
    ..Because inhibitors used in purification of ChEs by affinity chromatography are charged, CE remains a leading method to estimate enzyme stability and detect the presence of bound hidden ligands...
  85. ncbi Detection of unwanted protein-bound ligands by capillary zone electrophoresis: the case of hidden ligands that stabilize cholinesterase conformation
    Daniel Rochu
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, BP 87, F 38702 La Tronche Cedex, France
    Electrophoresis 23:930-7. 2002
    ..Finally, CZE was proved to be a pertinent tool to validate the conformity of purified enzymes to a status of biopharmaceutical...
  86. doi Application of laccase-mediator system (LMS) for the degradation of organophosphorus compounds
    Marie Trovaslet-Leroy
    Departement de Toxicologie, Centre de Recherches du Service de Santé des Armées CRSSA, 24 Avenue des Maquis du Gresivaudan, 38702 La Tronche Cedex, France
    Chem Biol Interact 187:393-6. 2010
    ..Altogether, results underline the importance of the OP nitrogen atom at beta-position and of its substituents, even though the intimate mechanism of laccase-catalyzed degradation is not yet known...
  87. ncbi Mutant of Bungarus fasciatus acetylcholinesterase with low affinity and low hydrolase activity toward organophosphorus esters
    Thomas Poyot
    Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, BP 87, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1764:1470-8. 2006
    ..DFP was characterised as a slow-binding substrate. This mutant is the first mutant of AChE capable of hydrolysing organophosphates. However, the overall OPase efficiency was greatly decreased compared to G117H butyrylcholinesterase...
  88. doi Integrative analytical approach by capillary electrophoresis and kinetics under high pressure optimized for deciphering intrinsic and extrinsic cofactors that modulate activity and stability of human paraoxonase (PON1)
    Frédérique Renault
    Departement de Toxicologie, Institut de Recherche Biomédicale des Armées, Antenne La Tronche CRSSA, BP 87, 38702 La Tronche Cedex, France
    J Chromatogr B Analyt Technol Biomed Life Sci 878:1346-55. 2010
    ..Relevance of these data to understand in vivo physiological PON1 functioning is mandatory...
  89. ncbi The wild type bacterial Co(2+)/Co(2+)-phosphotriesterase shows a middle-range thermostability
    Daniel Rochu
    Unite d Enzymologie, Centre de Recherches du Service de Sante des Armees, P O Box 87, 38702 La Tronche Cedex, France
    Biochim Biophys Acta 1594:207-18. 2002
    ..Hence, for decontamination purposes under extreme Earth temperatures, wild type and engineered mutants of PTE substituted with other metal cations should be evaluated...
  90. pmc Combined effects of high hydrostatic pressure and temperature for inactivation of Bacillus anthracis spores
    Cécile Cléry-Barraud
    Departement de Toxicologie, Centre de Recherches du Service de Sante des Armees, 38702 La Tronche Cedex, France
    Appl Environ Microbiol 70:635-7. 2004
    ..1 MPa) and 75 degrees C. The use of high pressure for spore inactivation represents a considerable improvement over other available methods of spore inactivation and could be of interest for antigenic spore preparation...
  91. ncbi Crystal structure of human butyrylcholinesterase and of its complexes with substrate and products
    Yvain Nicolet
    Laboratoire de Cristallographie et Cristallogenese des Proteines, Institut de Biologie Structurale Jean Pierre Ebel, CEA, UJF, CNRS, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    J Biol Chem 278:41141-7. 2003
    ..An electron density peak close to the catalytic Ser(198) has been modeled as bound butyrate...
  92. ncbi Improved access to 2-O-monobenzyl ethers of beta-cyclodextrin as precursors of catalysts for organophosphoryl esters hydrolysis
    Nicolas Masurier
    UMR 6014 CNRS, Universite de Rouen, INSA de Rouen, Laboratoire de Pharmacochimie, U F R de Médecine et de Pharmacie, 22 Boulevard Gambetta, F 76183 Rouen, France
    Carbohydr Res 341:935-40. 2006
    ....
  93. doi Structural basis for natural lactonase and promiscuous phosphotriesterase activities
    Mikael Elias
    Laboratoire de Cristallographie et Modélisation des Matériaux Minéraux et Biologiques, CNRS Université Henri Poincaré, 54506 Nancy, France
    J Mol Biol 379:1017-28. 2008
    ..This example demonstrates that promiscuous activities probably constitute a large and efficient reservoir for the creation of novel catalytic activities...
  94. ncbi Concentration-dependent reversible activation-inhibition of human butyrylcholinesterase by tetraethylammonium ion
    Jure Stojan
    Institute of Biochemistry, Medical Faculty, University of Ljubljana, Slovenia
    Eur J Biochem 269:1154-61. 2002
    ..At the concentrations around 40 mm, well within the range of TEA competition at both substrate binding sites, it lowers the activity of all tested enzymes...
  95. ncbi Synthesis of 2-substituted beta-cyclodextrin derivatives with a hydrolytic activity against the organophosphorylester paraoxon
    Nicolas Masurier
    Laboratoire de Pharmacochimie, département de chimie organique pharmaceutique, Faculte de medecine et de pharmacie de Rouen, 22 Boulevard Gambetta, 76183 Rouen Cedex 1, France
    Eur J Med Chem 40:615-23. 2005
    ..Kinetic assays were carried out with paraoxon as organophosphate model. Three regioisomers hydrolyzed paraoxon, although the paraoxon-leaving group, para-nitrophenol, was not released from the beta-cyclodextrin torus...
  96. ncbi Photoreversible inhibition of cholinesterases: catalytic serine-labeled caged butyrylcholinesterase
    Sandra Loudwig
    Laboratoire de Chimie Bioorganique, UMR 7514 CNRS, Faculte de Pharmacie, Universite Louis Pasteur Strasbourg, B P 24, 67401 Illkirch Cedex, France
    Chembiochem 4:762-7. 2003
    ....
  97. pmc The reactant state for substrate-activated turnover of acetylthiocholine by butyrylcholinesterase is a tetrahedral intermediate
    Jose R Tormos
    Department of Chemistry, The University of Iowa, Iowa City, Iowa 52242, USA
    J Am Chem Soc 127:14538-9. 2005
    ..Hence, the catalytic power of cholinesterases can largely be understood in terms of their ability to stabilize tetrahedral intermediates in the multistep reaction mechanism...
  98. ncbi Use of a 'caged' analogue to study the traffic of choline within acetylcholinesterase by kinetic crystallography
    Jacques Philippe Colletier
    Laboratoire de Biophysique Moleculaire, Institut de Biologie Structurale Jean Pierre Ebel, UMR 5075 CNRS CEA UJF, 38027 Grenoble, France
    Acta Crystallogr D Biol Crystallogr 63:1115-28. 2007
    ..This structural characterization of acetylcholinesterase 'at work' is consistent with the idea that choline exits from the enzyme after catalysis either via the gorge or via an alternative 'backdoor' trajectory...
  99. pmc Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus solfataricus phosphotriesterase
    Mikael Elias
    Laboratoire de Cristallographie et Modélisation des Matériaux Minéraux et Biologiques, CNRS Université Henri Poincaré, 54506 Vandoeuvre les Nancy, France
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:553-5. 2007
    ..Here, the crystallization conditions and data collection for the hyperthermophilic S. solfataricus PTE are reported...
  100. ncbi High pressure effects on biological macromolecules: from structural changes to alteration of cellular processes
    Claude Balny
    INSERM Unite 128, IFR 24, CNRS, 1919, route de Mende, Montpellier, France
    Biochim Biophys Acta 1595:3-10. 2002
  101. pmc Use of RNA interference and complementation to study the function of the Drosophila and human 26S proteasome subunit S13
    Josefin Lundgren
    Department of Molecular Biology and Functional Genetics, Stockholm University, S 10691 Stockholm, Sweden
    Mol Cell Biol 23:5320-30. 2003
    ..The S13 subunit may possess multiple functions, including a deubiquitinylating activity and distinct activities essential for cell cycle progression that require the conserved C120 residue...