WILLIAM F contact MARZLUFF

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Histones: sequestered by Jabba in fatty storehouse
    William F Marzluff
    Department of Biochemistry, University of North Carolina at Chapel Hill School of Medicine, 208 Fordham Hall, Campus Box 7100, Chapel Hill, NC 27599, USA
    Curr Biol 22:R951-3. 2012
  2. pmc A unified phylogeny-based nomenclature for histone variants
    Paul B Talbert
    Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA
    Epigenetics Chromatin 5:7. 2012
  3. pmc Metabolism and regulation of canonical histone mRNAs: life without a poly(A) tail
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Rev Genet 9:843-54. 2008
  4. pmc Translation termination is involved in histone mRNA degradation when DNA replication is inhibited
    Handan Kaygun
    Department of Biology, University of North Carolina, Chapel Hill, 27599, USA
    Mol Cell Biol 25:6879-88. 2005
  5. ncbi request reprint Metazoan replication-dependent histone mRNAs: a distinct set of RNA polymerase II transcripts
    William F Marzluff
    Program in Molecular Biology and Biotechnology, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Curr Opin Cell Biol 17:274-80. 2005
  6. ncbi request reprint The sea urchin histone gene complement
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Dev Biol 300:308-20. 2006
  7. ncbi request reprint The human and mouse replication-dependent histone genes
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, USA
    Genomics 80:487-98. 2002
  8. pmc Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, at the end of S phase
    Lianxing Zheng
    Department of Biochemistry and Biophysics Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:1590-601. 2003
  9. pmc 3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing system
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell Biol 22:6648-60. 2002
  10. pmc Drosophila stem-loop binding protein intracellular localization is mediated by phosphorylation and is required for cell cycle-regulated histone mRNA expression
    David J Lanzotti
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Biol Cell 15:1112-23. 2004

Research Grants

Collaborators

Detail Information

Publications66

  1. pmc Histones: sequestered by Jabba in fatty storehouse
    William F Marzluff
    Department of Biochemistry, University of North Carolina at Chapel Hill School of Medicine, 208 Fordham Hall, Campus Box 7100, Chapel Hill, NC 27599, USA
    Curr Biol 22:R951-3. 2012
    ..In Jabba mutant embryos, histones H2a and H2b are degraded but embryos survive by translating stored histone mRNA...
  2. pmc A unified phylogeny-based nomenclature for histone variants
    Paul B Talbert
    Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA
    Epigenetics Chromatin 5:7. 2012
    ..For clarity and searchability, we encourage the use of descriptors that are separate from the phylogeny-based variant name to indicate developmental and other properties of variants that may be independent of structure...
  3. pmc Metabolism and regulation of canonical histone mRNAs: life without a poly(A) tail
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Rev Genet 9:843-54. 2008
    ..Recent results suggest that the coordinated synthesis of replication-dependent and variant histone mRNAs is achieved by signals that affect formation of the 3' end of the replication-dependent histone mRNAs...
  4. pmc Translation termination is involved in histone mRNA degradation when DNA replication is inhibited
    Handan Kaygun
    Department of Biology, University of North Carolina, Chapel Hill, 27599, USA
    Mol Cell Biol 25:6879-88. 2005
    ..We show here that the stability of histone mRNAs can be modified by altering the position of the stem-loop, thereby changing the distance from the translation termination codon...
  5. ncbi request reprint Metazoan replication-dependent histone mRNAs: a distinct set of RNA polymerase II transcripts
    William F Marzluff
    Program in Molecular Biology and Biotechnology, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Curr Opin Cell Biol 17:274-80. 2005
    ..Recently several novel factors, including components of the U7 snRNP, as well as proteins involved in regulation of histone gene expression, have been described...
  6. ncbi request reprint The sea urchin histone gene complement
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Dev Biol 300:308-20. 2006
    ..The cleavage stage histone H1 is the orthologue of an oocyte-specific histone H1 protein found in vertebrates...
  7. ncbi request reprint The human and mouse replication-dependent histone genes
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, USA
    Genomics 80:487-98. 2002
    ..In contrast, both the mouse and human H2a and H2b proteins consist of at least 10 non-allelic variants, making the complexity of the histone protein complement significantly greater than previously thought...
  8. pmc Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, at the end of S phase
    Lianxing Zheng
    Department of Biochemistry and Biophysics Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:1590-601. 2003
    ..Nuclear extracts from G1 and G2 cells are deficient in histone pre-mRNA processing, which is restored by addition of recombinant SLBP, indicating that SLBP is the only cell cycle-regulated factor required for histone pre-mRNA processing...
  9. pmc 3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing system
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell Biol 22:6648-60. 2002
    ..20 nucleotides downstream from the stem, and an Sm-reactive factor, most likely the Drosophila counterpart of vertebrate U7 snRNP...
  10. pmc Drosophila stem-loop binding protein intracellular localization is mediated by phosphorylation and is required for cell cycle-regulated histone mRNA expression
    David J Lanzotti
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Biol Cell 15:1112-23. 2004
    ..The T230A protein is concentrated in the cytoplasm, suggesting that it is defective in nuclear targeting, and accounting for its failure to function in histone pre-mRNA processing in vivo...
  11. pmc A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3'-end processing
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Genes Dev 16:58-71. 2002
    ..Antibodies to hZFP100 precipitate U7 snRNA, and expression of hZFP100 in Xenopus oocytes stimulates processing of histone pre-mRNA, showing that hZFP100 is a component of the processing machinery...
  12. pmc Developmental control of histone mRNA and dSLBP synthesis during Drosophila embryogenesis and the role of dSLBP in histone mRNA 3' end processing in vivo
    David J Lanzotti
    Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell Biol 22:2267-82. 2002
    ..There is little or no dSLBP protein provided maternally in wild-type embryos, and zygotic expression of dSLBP is immediately required to process newly made histone pre-mRNA...
  13. pmc SLIP1, a factor required for activation of histone mRNA translation by the stem-loop binding protein
    Nihal G Cakmakci
    Program in Molecular Biology and Biotechnology, CB 7100, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 28:1182-94. 2008
    ..SLIP1 may function by bridging the 3' end of the histone mRNA with the 5' end of the mRNA, similar to the mechanism of translation of polyadenylated mRNAs...
  14. pmc Nuclear import of the stem-loop binding protein and localization during the cell cycle
    Judith A Erkmann
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 16:2960-71. 2005
    ..In contrast, we were unable to identify an in vivo role for Transportin-SR2 in SLBP nuclear localization. Thus, only the Impalpha/Impbeta pathway contributes to SLBP nuclear import in HeLa cells...
  15. pmc ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase
    Eric J Wagner
    Program in Molecular Biology and Biotechnology, CB 7100, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 26:6702-12. 2006
    ....
  16. pmc Nuclear export of metazoan replication-dependent histone mRNAs is dependent on RNA length and is mediated by TAP
    Judith A Erkmann
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    RNA 11:45-58. 2005
    ..Consistent with this observation, depletion of TAP from Drosophila cells by RNAi resulted in the restriction of mature histone mRNAs to the nucleus...
  17. pmc The sea urchin stem-loop-binding protein: a maternally expressed protein that probably functions in expression of multiple classes of histone mRNA
    Anthony J Robertson
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill 27599, USA
    Nucleic Acids Res 32:811-8. 2004
    ..SuSLBP expressed by in vitro translation does not bind the stem-loop RNA, suggesting that suSLBP is modified to activate RNA binding in sea urchin embryos...
  18. pmc Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing
    Xiao cui Yang
    Program in Molecular Biology and Biotechnology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 29:31-42. 2009
    ..RNA interference experiments with HeLa cells indicate that degradation of the DCP does not depend on the Xrn2 5' exonuclease, suggesting that CPSF-73 degrades the DCP both in vitro and in vivo...
  19. ncbi request reprint A genome-wide RNA interference screen reveals that variant histones are necessary for replication-dependent histone pre-mRNA processing
    Eric J Wagner
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 28:692-9. 2007
    ....
  20. pmc FLASH, a proapoptotic protein involved in activation of caspase-8, is essential for 3' end processing of histone pre-mRNAs
    Xiao cui Yang
    Department of Biochemistry and Biophysics and Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 36:267-78. 2009
    ..These results demonstrate that FLASH is an essential factor required for 3' end maturation of histone mRNAs in both vertebrates and invertebrates and suggest a potential link between this process and apoptosis...
  21. pmc A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs
    Kelly D Sullivan
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 34:322-32. 2009
    ..These results suggest that a common core cleavage factor is required for processing of histone and polyadenylated pre-mRNAs...
  22. pmc SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP
    Michael L Whitfield
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 32:4833-42. 2004
    ..SLBP remains active both in RNA binding and histone pre-mRNA processing when DNA replication is inhibited...
  23. pmc Genetic and biochemical characterization of Drosophila Snipper: A promiscuous member of the metazoan 3'hExo/ERI-1 family of 3' to 5' exonucleases
    Jeremy M Kupsco
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    RNA 12:2103-17. 2006
    ..Therefore, Snp is a nonessential exonuclease that is not a functional ortholog of either 3'hExo or ERI-1...
  24. pmc Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'
    Thomas E Mullen
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Genes Dev 22:50-65. 2008
    ..RNAi experiments demonstrate that both the exosome and 5'-to-3' decay pathway components are required for degradation, and individual histone mRNAs are then degraded simultaneously 5' to 3' and 3' to 5'...
  25. pmc Three proteins of the U7-specific Sm ring function as the molecular ruler to determine the site of 3'-end processing in mammalian histone pre-mRNA
    Xiao cui Yang
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 29:4045-56. 2009
    ..These proteins likely rigidify the substrate and function as the molecular ruler in determining the site of cleavage...
  26. ncbi request reprint Characterization of 3'hExo, a 3' exonuclease specifically interacting with the 3' end of histone mRNA
    Xiao cui Yang
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 281:30447-54. 2006
    ..3'hExo removes 3' overhangs of small interfering RNAs, whereas the double-stranded region is resistant to the enzymatic activity...
  27. pmc Developmental and cell cycle regulation of the Drosophila histone locus body
    Anne E White
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 18:2491-502. 2007
    ..HLB foci are present in histone deletion embryos, although the MPM-2 foci are smaller, and some Lsm11 foci are not associated with MPM-2 foci, suggesting that the histone locus is important for HLB integrity...
  28. ncbi request reprint Histone mRNA expression: multiple levels of cell cycle regulation and important developmental consequences
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Curr Opin Cell Biol 14:692-9. 2002
    ..The recent identification of several novel proteins involved in histone gene transcription and pre-mRNA processing has shed light on the variety of mechanisms cells employ to achieve this coupling...
  29. pmc Phosphorylation of threonine 61 by cyclin a/Cdk1 triggers degradation of stem-loop binding protein at the end of S phase
    M Murat Koseoglu
    Department of Biology, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 28:4469-79. 2008
    ..We conclude that the increase in cyclin A/Cdk1 activity at the end of S phase triggers degradation of SLBP at S/G(2)...
  30. pmc U7 snRNA mutations in Drosophila block histone pre-mRNA processing and disrupt oogenesis
    Ashley C Godfrey
    Department of Biology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
    RNA 12:396-409. 2006
    ..These data suggest that SLBP and U7 snRNP cooperate in the production of histone mRNA in vivo, and that disruption of histone pre-mRNA processing is detrimental to development...
  31. pmc Knockdown of SLBP results in nuclear retention of histone mRNA
    Kelly D Sullivan
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    RNA 15:459-72. 2009
    ..The processed histone mRNA in SLBP knockdown cells is not rapidly degraded when DNA replication is inhibited. These results suggest a previously undescribed role for SLBP in histone mRNA export...
  32. ncbi request reprint Electrostatic contribution of serine phosphorylation to the Drosophila SLBP--histone mRNA complex
    Roopa Thapar
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 43:9401-12. 2004
    ..Hence, both RNA binding and protein phosphorylation are necessary for stabilization of the SLBP RPD...
  33. pmc The oligo(A) tail on histone mRNA plays an active role in translational silencing of histone mRNA during Xenopus oogenesis
    Ricardo Sanchez
    Program in Molecular Biology and Biotechnology, Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 24:2513-25. 2004
    ..These results suggest that the oligo(A) tail is an active part of the translation repression mechanism that silences histone mRNA during oogenesis and that its removal is part of the mechanism that activates translation...
  34. pmc The stem-loop binding protein is required for efficient translation of histone mRNA in vivo and in vitro
    Ricardo Sanchez
    Program in Molecular Biology and Biotechnology, Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 22:7093-104. 2002
    ....
  35. pmc The Drosophila U7 snRNP proteins Lsm10 and Lsm11 are required for histone pre-mRNA processing and play an essential role in development
    Ashley C Godfrey
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    RNA 15:1661-72. 2009
    ....
  36. pmc Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein
    Eric J Wagner
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    RNA 12:1206-18. 2006
    ..Comparisons with other mammalian ZFP100 orthologs show that the central Zn fingers sufficient for in vivo activity are most highly conserved, whereas the number and sequence of the Zn fingers in the N- and C-terminal domains vary...
  37. ncbi request reprint The N-terminal domain of the Drosophila histone mRNA binding protein, SLBP, is intrinsically disordered with nascent helical structure
    Roopa Thapar
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 43:9390-400. 2004
    ..The implications of this unfolded state for the function of dSLBP in regulating histone metabolism are discussed...
  38. pmc Cloning and characterization of the Drosophila U7 small nuclear RNA
    Zbigniew Dominski
    Department of Biochemistry and Biophysics and Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 100:9422-7. 2003
    ..Changes in the HDE that abolish or decrease processing efficiency result in a reduced ability to recruit U7 snRNA to the pre-mRNA...
  39. ncbi request reprint U2 snRNP: not just for poly(A) mRNAs
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell 28:353-4. 2007
    ..Friend et al. (2007) now report that the U2 snRNP, required for pre-mRNA splicing, is also required for histone mRNA 3' end formation...
  40. pmc Formation of the 3' end of histone mRNA: getting closer to the end
    Zbigniew Dominski
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Gene 396:373-90. 2007
    ..The greatest challenge that lies ahead is to determine how all these factors interact with each other to form a catalytically competent processing complex capable of cleaving histone pre-mRNAs...
  41. pmc Differences and similarities between Drosophila and mammalian 3' end processing of histone pre-mRNAs
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    RNA 11:1835-47. 2005
    ....
  42. ncbi request reprint A 3' exonuclease that specifically interacts with the 3' end of histone mRNA
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell 12:295-305. 2003
    ..These features make 3'hExo a primary candidate for the exonuclease that initiates rapid decay of histone mRNA upon completion and/or inhibition of DNA replication...
  43. pmc Crystal structure of the HEAT domain from the Pre-mRNA processing factor Symplekin
    Sarah A Kennedy
    Department of Chemistry, University of North Carolina at Chapel Hill, 27599, USA
    J Mol Biol 392:115-28. 2009
    ..Together, these data support the conclusion that the Symplekin HEAT domain serves as a scaffold for protein-protein interactions essential to the mRNA maturation process...
  44. pmc Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding
    Christoph H Borchers
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, 27599, USA
    Proc Natl Acad Sci U S A 103:3094-9. 2006
    ..Removal of the phosphoryl group from T230 by either dephosphorylation or mutation results in a 7-fold reduction in the affinity of SLBP for the stem-loop RNA...
  45. ncbi request reprint The polyadenylation factor CPSF-73 is involved in histone-pre-mRNA processing
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Cell 123:37-48. 2005
    ..These studies suggest that CPSF-73 is both the endonuclease and 5'-3' exonuclease in histone-pre-mRNA processing and reveal an evolutionary link between 3' end formation of histone mRNAs and polyadenylated mRNAs...
  46. doi request reprint Terminating histone synthesis to preserve centromere integrity
    William F Marzluff
    The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Dev Cell 18:335-6. 2010
    ..In this issue of Developmental Cell, Takayama and coworkers elucidate a mechanism for silencing histone expression at the end of S phase in S. pombe. Failure to shut off histone expression disrupts centromeric chromatin structure...
  47. ncbi request reprint Regulated degradation of replication-dependent histone mRNAs requires both ATR and Upf1
    Handan Kaygun
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Nat Struct Mol Biol 12:794-800. 2005
    ..Here we show that regulated degradation of histone mRNAs requires Upf1, a key regulator of the nonsense-mediated decay pathway, and ATR, a key regulator of the DNA damage checkpoint pathway activated during replication stress...
  48. pmc A CPSF-73 homologue is required for cell cycle progression but not cell growth and interacts with a protein having features of CPSF-100
    Zbigniew Dominski
    Program in Molecular Biology and Biotechnology, CB 3280, University of North Carolina, Chapel Hill, NC 27599
    Mol Cell Biol 25:1489-500. 2005
    ..RC-68 is highly conserved from plants to humans and may function in conjunction with RC-74 in the 3' end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase...
  49. pmc FLASH is required for the endonucleolytic cleavage of histone pre-mRNAs but is dispensable for the 5' exonucleolytic degradation of the downstream cleavage product
    Xiao cui Yang
    Department of Biochemistry and Biophysics, Program in Molecular Biology and Biotechnology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 31:1492-502. 2011
    ..These results suggest that CPSF-73, the catalytic component in both reactions, can be recruited to histone pre-mRNA largely in a manner independent of FLASH, possibly by a separate domain in Lsm11...
  50. doi request reprint Chapter 2. Cell-cycle regulation of histone mRNA degradation in Mammalian cells: role of translation and oligouridylation
    Thomas E Mullen
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina, USA
    Methods Enzymol 449:23-45. 2008
    ..In particular, the initial step in histone mRNA degradation is attachment of an oligo(U) tail to the 3' end of histone mRNA, providing a platform for binding factors that trigger mRNA degradation...
  51. ncbi request reprint string(cdc25) and cyclin E are required for patterned histone expression at different stages of Drosophila embryonic development
    David J Lanzotti
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Dev Biol 274:82-93. 2004
    ..Thus, during the altered cell cycles of early animal development, different cell cycle mechanisms are employed to ensure that the production of histones accompanies DNA synthesis...
  52. ncbi request reprint Nuclear autoantigenic sperm protein (NASP), a linker histone chaperone that is required for cell proliferation
    Richard T Richardson
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, 27599, USA
    J Biol Chem 281:21526-34. 2006
    ..We conclude from this study that NASP and therefore the linker histones are key players in the assembly of chromatin after DNA replication...
  53. ncbi request reprint Phosphatase-directed phosphorylation-site determination: a synthesis of methods for the detection and identification of phosphopeptides
    Matthew P Torres
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    J Proteome Res 4:1628-35. 2005
    ....
  54. pmc Cyclin E in centrosome duplication and reduplication in sea urchin zygotes
    Bradley J Schnackenberg
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Physiol 217:626-31. 2008
    ..This indicates that centrosome duplication during the G1 arrest is limited by a block to reduplication under conditions permissive for duplication. The cytoplasmic conditions of S phase, however, abrogate this block to reduplication...
  55. ncbi request reprint Direct MALDI-MS/MS of phosphopeptides affinity-bound to immobilized metal ion affinity chromatography beads
    Christina S Raska
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 27599, USA
    Anal Chem 74:3429-33. 2002
    ..Direct analysis of phosphorylated peptides on IMAC beads does not adversely affect the high-mass accuracy of an orthogonal injection QqTOF mass spectrometer, making it a suitable technique for phosphoproteomics...
  56. pmc Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos
    Jennifer C Moore
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 22:4863-75. 2002
    ..These results suggest that in sea urchins, cyclin D and cdk4 are required for normal development and perhaps the patterning of the developing embryo, but may not be directly involved in regulating entry into the cell cycle...
  57. ncbi request reprint Novel localization and possible functions of cyclin E in early sea urchin development
    Bradley J Schnackenberg
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Sci 115:113-21. 2002
    ..Furthermore, cyclin E does not need to be degraded or dissociated from the chromosomes during mitosis; instead, it may be required on chromosomes during mitosis to immediately initiate the next round of DNA replication...
  58. ncbi request reprint Cyclin E/Cdk2 is required for sperm maturation, but not DNA replication, in early sea urchin embryos
    Bradley J Schnackenberg
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Genesis 45:282-91. 2007
    ..We conclude that cyclin E/cdk2 activity is required for male pronuclear maturation, but not for initiation of DNA replication in early sea urchin development...
  59. pmc A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
    Helen X Wang
    Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Mol Biol Cell 17:4257-69. 2006
    ..SGB1 is shown here to be a Golgi-localized hexose transporter and acts genetically with AGB1 in early seedling development...
  60. pmc NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop binding protein
    Eric S DeJong
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251 1892, USA
    RNA 8:83-96. 2002
    ..The flanking nucleotides at the base of the hairpin stem do not assume a unique conformation, despite the fact that the 5' flanking nucleotides are a critical component of the SLBP binding site...
  61. pmc Genome-wide analysis of mRNAs bound to the histone stem-loop binding protein
    W H Davin Townley-Tilson
    Department of Genetics, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    RNA 12:1853-67. 2006
    ..Both platforms demonstrate remarkable specificity and consistency of results. Our data suggest that the replication-dependent histone mRNAs are likely to be the sole target of SLBP...
  62. ncbi request reprint Stem-loop binding protein accumulates during oocyte maturation and is not cell-cycle-regulated in the early mouse embryo
    Patrick Allard
    Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada H3A 1A1
    J Cell Sci 115:4577-86. 2002
    ..This distinctive pattern of SLBP expression may be required for accumulation of histone proteins required for sperm chromatin remodelling and assembly of newly synthesized embryonic DNA into chromatin...
  63. pmc The histone 3'-terminal stem-loop-binding protein enhances translation through a functional and physical interaction with eukaryotic initiation factor 4G (eIF4G) and eIF3
    Jun Ling
    Department of Biochemistry, University of California, Riverside, California 92521 0129, USA
    Mol Cell Biol 22:7853-67. 2002
    ..These data indicate that SLBP stimulates the translation of histone mRNAs through a functional interaction with both the mRNA stem-loop and the 5' cap that is mediated by eIF4G and eIF3...
  64. ncbi request reprint The stem-loop binding protein regulates translation of histone mRNA during mammalian oogenesis
    Patrick Allard
    Department of Biology, McGill University, Montreal, QC, Canada
    Dev Biol 286:195-206. 2005
    ..These results demonstrate that histone synthesis in immature and maturing oocytes is governed by a translational control mechanism that is directly regulated by changes in the amount of SLBP...
  65. ncbi request reprint The genomic repertoire for cell cycle control and DNA metabolism in S. purpuratus
    Antonio Fernandez-Guerra
    Observatoire Océanologique de Banyuls Laboratoire Arago, CNRS UMR7628 UPMC, 66650 Banyuls sur mer, France
    Dev Biol 300:238-51. 2006
    ..The set of genes uncovered in this analysis of the S. purpuratus genome should enhance future research on cell cycle control and developmental regulation in this model...
  66. ncbi request reprint Cyclin B synthesis is required for sea urchin oocyte maturation
    Ekaterina Voronina
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Dev Biol 256:258-75. 2003
    ..We also find that cyclin A can functionally substitute for cyclin B in early embryos but not in oocytes. These studies provide a foundation for understanding the mechanism of meiotic maturation independent of the zygotic cell cycle...

Research Grants50

  1. HISTONE MRNA REGULATION IN DEVELOPMENT
    Zbigniew Dominski; Fiscal Year: 2011
    ..This novel protein, SLIP1, is likely involved in translation regulation of other mRNAs, and we will identify what other pathways it participates in. ..
  2. UNC-Chapel Hill Integrated Biomedical Research Training Program
    William Marzluff; Fiscal Year: 2007
    ..S. or Ph.D. in their chosen discipline. Participating departments have committed to accommodate students with flexible timelines for qualifying exams,and an expanded selectionofelective courses. ..
  3. UNC-Chapel Hill Integrated Biomedical Research Training Program
    William Marzluff; Fiscal Year: 2007
    ..S. or PhD in their chosen discipline. Participating departments have committed to accommodate students with flexible timelines for qualifying exams, and an expanded selection of elective courses. ..
  4. Post-transcriptional Control of Gene Expression: Mechanisms of mRNA Decay
    William Marzluff; Fiscal Year: 2007
    ..In short, the conference will provide an overview of the latest progress in an exciting field and will propel the next stage of its development. ..
  5. Screen for molecules inhibiting histone pre-mRNA process
    William Marzluff; Fiscal Year: 2006
    ..We are adapting the screen to an automated microscope which can rapidly read the 384 well plates. ..
  6. Phosphorylation dependent recognition of a histone mRNA hairpin by SLBP
    William Marzluff; Fiscal Year: 2007
    ....
  7. CONTROL OF HISTONE MRNA LEVELS
    William Marzluff; Fiscal Year: 2006
    ..SLBP degradation is activated at the end of S phase as a result of phosphorylation of two specific threonines. The kinase responsible for this phosphorylation will be identified. ..
  8. HISTONE MRNA REGULATION IN DEVELOPMENT
    William Marzluff; Fiscal Year: 2007
    ..Using genetics, we will identify novel genes involved in biosynthesis and regulation of histone mRNA levels. ..
  9. CONTROL OF HISTONE MRNA LEVELS
    William F Marzluff; Fiscal Year: 2010
    ..We will determine the factors critical for both histone mRNA synthesis and degradation, and novel factors provide potential new chemotherapy targets, as well as helping us to understand the control of cell growth. ..
  10. Phosphorylation dependent recognition of a histone mRNA hairpin by SLBP
    William Marzluff; Fiscal Year: 2009
    ..abstract_text> ..
  11. Mitotic Checkpoint Regulators: Roles in Development and Cancer
    William F Marzluff; Fiscal Year: 2010
    ....
  12. CONTROL OF HISTONE MRNA LEVELS
    William F Marzluff; Fiscal Year: 2010
    ..We will determine the factors critical for both histone mRNA synthesis and degradation, and novel factors provide potential new chemotherapy targets, as well as helping us to understand the control of cell growth. ..
  13. HISTONE MRNA REGULATION IN DEVELOPMENT
    WILLIAM F contact MARZLUFF; Fiscal Year: 2010
    ..This novel protein, SLIP1, is likely involved in translation regulation of other mRNAs, and we will identify what other pathways it participates in. ..
  14. HISTONE MRNA REGULATION IN DEVELOPMENT
    William Marzluff; Fiscal Year: 2006
    ..Using genetics, we will identify novel genes involved in biosynthesis and regulation of histone mRNA levels. ..
  15. CONTROL OF HISTONE MRNA LEVELS
    William Marzluff; Fiscal Year: 1993
    ....
  16. HIGH THROUGHPUT GENOME ANALYSIS SYSTEM
    William Marzluff; Fiscal Year: 2001
    ..Together, these five objectives delineate a research strategy that will allow resolution of the seemingly contradictory evidence relating aluminum to neurological degeneration. ..
  17. CONTROL OF HISTONE MRNA LEVELS
    William Marzluff; Fiscal Year: 2002
    ..5. Using in situ methods, determine whether the two clusters of histone genes are colocalized with the U7 snRNP in the nucleus as a function of the cell cycle. ..