Research Topics
Species | THOMAS F MARTINSummaryAffiliation: University of Wisconsin Country: USA Publications
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Publications
Phosphatidylinositol 4,5-bisphosphate regulates SNARE-dependent membrane fusionDeclan J James
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
J Cell Biol 182:355-66. 2008..These results indicate that PI 4,5-P(2) regulates fusion both as a fusion restraint that syntaxin-1 alleviates and as an essential cofactor that recruits protein priming factors to facilitate SNARE-dependent fusion...
Docked secretory vesicles undergo Ca2+-activated exocytosis in a cell-free systemT F Martin
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
J Biol Chem 272:14447-53. 1997..A cell-free system for Ca2+-activated fusion will facilitate studies on the roles of essential proteins such as syntaxin, synaptobrevin, SNAP-25, and CAPS that act at post-docking steps in the regulated exocytotic pathway...- Phosphoinositides as spatial regulators of membrane trafficT F Martin
Department of Biochemistry, University of Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706, USA
Curr Opin Neurobiol 7:331-8. 1997..The roles played by phosphoinositides in aspects of secretory granule formation, fusion and endocytosis indicate the importance of phosphorylated lipids for neurotransmitter release... - Phosphoinositide lipids as signaling molecules: common themes for signal transduction, cytoskeletal regulation, and membrane traffickingT F Martin
Department of Biochemistry, University of Wisconsin, Madison 53706, USA
Annu Rev Cell Dev Biol 14:231-64. 1998..Common themes of localized signal generation and the spatially localized recruitment of effector proteins appear to underlie mechanisms employed in signal transduction, cytoskeletal, and membrane trafficking events... - PI(4,5)P(2) regulation of surface membrane trafficT F Martin
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, 53706, Wisconsin, USA
Curr Opin Cell Biol 13:493-9. 2001..The targeting of phosphoinositide kinases by GTPases can coordinate the reactions of membrane fusion and fission with cytoskeletal assembly, providing a basis for membrane movement... - Tuning exocytosis for speed: fast and slow modesThomas F J Martin
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
Biochim Biophys Acta 1641:157-65. 2003.... - Prime movers of synaptic vesicle exocytosisT F J Martin
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
Neuron 34:9-12. 2002..This work sheds new light on how presynaptic structure provides speed and plasticity to synaptic transmission... - Synaptotagmin-1 utilizes membrane bending and SNARE binding to drive fusion pore expansionKara L Lynch
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
Mol Biol Cell 19:5093-103. 2008..The results indicate that Syt-1 uses both Ca(2+)-dependent membrane insertion and SNARE binding to drive fusion pore expansion... - Imaging of evoked dense-core-vesicle exocytosis in hippocampal neurons reveals long latencies and kiss-and-run fusion eventsXiaofeng Xia
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
J Cell Sci 122:75-82. 2009..Thus, the slow evoked release of neuropeptides could be attributed to very prolonged latencies from stimulation to fusion and transient fusion-pore openings that might limit cargo secretion... - Ca2+-dependent synaptotagmin binding to SNAP-25 is essential for Ca2+-triggered exocytosisXiaodong Zhang
Department of Biochemistry, University of Wisconsin, Madison 53706, USA
Neuron 34:599-611. 2002..These results indicate that the Ca2+-dependent interaction of synaptotagmin with SNAP-25 is essential for the Ca2+-dependent triggering of membrane fusion... - Membrane association domains in Ca2+-dependent activator protein for secretion mediate plasma membrane and dense-core vesicle binding required for Ca2+-dependent exocytosisRuslan N Grishanin
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
J Biol Chem 277:22025-34. 2002..The presence of two membrane association domains with distinct binding specificities may enable CAPS to bind both target membranes to facilitate DCV-plasma membrane fusion... - ARF6 regulates a plasma membrane pool of phosphatidylinositol(4,5)bisphosphate required for regulated exocytosisYoshikatsu Aikawa
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
J Cell Biol 162:647-59. 2003..We conclude that ARF6 plays a role in Ca2+-dependent DCV exocytosis by regulating the activity of PIP5K for the synthesis of an essential plasma membrane pool of PIP2... - Different domains of synaptotagmin control the choice between kiss-and-run and full fusionChih-Tien Wang
Department of Physiology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Nature 424:943-7. 2003..Syt thus regulates the choice between full fusion and kiss-and-run, with Ca2+ binding to the C2A and C2B domains playing an important role in this choice... - CAPS acts at a prefusion step in dense-core vesicle exocytosis as a PIP2 binding proteinRuslan N Grishanin
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
Neuron 43:551-62. 2004..Regulation of PIP2 levels and CAPS-1 activity would control the secretion of neuropeptides and monoaminergic transmitters... - CAPS activity in priming vesicle exocytosis requires CK2 phosphorylationMari Nojiri
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
J Biol Chem 284:18707-14. 2009..These results identify a functionally important N-terminal phosphorylation site that regulates CAPS activity in priming vesicle exocytosis... - Synaptotagmins I and IX function redundantly in regulated exocytosis but not endocytosis in PC12 cellsKara L Lynch
Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706, USA
J Cell Sci 120:617-27. 2007..The downregulation of synaptotagmin I but not synaptotagmin IX impaired compensatory vesicle endocytosis, which revealed a lack of isoform redundancy and functional specialization of synaptotagmin I for endocytic retrieval... - Identification of synaptotagmin effectors via acute inhibition of secretion from cracked PC12 cellsWard C Tucker
Department of Physiology, University of Wisconsin, Madison, WI 53706, USA
J Cell Biol 162:199-209. 2003..These data suggest that syts trigger fusion via their Ca2+-regulated interactions with t-SNAREs and PIP2, target molecules known to play critical roles in exocytosis... - CAPS drives trans-SNARE complex formation and membrane fusion through syntaxin interactionsDeclan J James
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 106:17308-13. 2009..The results revealed an unexpected activity of a priming protein to accelerate fusion by efficiently promoting trans-SNARE complex formation. CAPS may function in priming by organizing SNARE complexes on the plasma membrane... - SNAP25, but not syntaxin 1A, recycles via an ARF6-regulated pathway in neuroendocrine cellsYoshikatsu Aikawa
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
Mol Biol Cell 17:711-22. 2006..Our results characterize a robust ARF6-dependent internalization mechanism that maintains an intracellular pool of SNAP25, which is compatible with possible intracellular roles for SNAP25 in neuroendocrine cells... - Role of CAPS in dense-core vesicle exocytosisJames J Wassenberg
Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Ann N Y Acad Sci 971:201-9. 2002..The results are consistent with a widespread functional role of CAPS in the regulated exocytosis of DCVs in the nervous and endocrine systems... - A family of Ca2+-dependent activator proteins for secretion: comparative analysis of structure, expression, localization, and functionDina Speidel
Department of Molecular Neurobiology, Max Planck Institute for Experimental Medicine and Deutsche Forschungsgemeinschaft Center for Molecular Physiology of the Brain, D 37075 Gottingen, Germany
J Biol Chem 278:52802-9. 2003..The abundance of CAPS proteins in synaptic terminals indicates that they may also be important for neuronal functions that are not exclusively related to LDCV exocytosis... - Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cellsMitsunori Fukuda
Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
J Biol Chem 277:4601-4. 2002..Our results support the idea that Syt family proteins that co-reside on secretory vesicles may function cooperatively and redundantly as potential Ca(2+) sensors for exocytosis... - UNC-31 (CAPS) is required for dense-core vesicle but not synaptic vesicle exocytosis in Caenorhabditis elegansSean Speese
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
J Neurosci 27:6150-62. 2007..Our results suggest that CAPS/UNC-31 and UNC-13 serve parallel and dedicated roles in dense-core vesicle and synaptic vesicle exocytosis, respectively, in the C. elegans nervous system... - G protein betagamma directly regulates SNARE protein fusion machinery for secretory granule exocytosisTrillium Blackmer
Department of Biological Sciences, University of Illinois at Chicago, 840 West Taylor Street, Chicago, Illinois 60607, USA
Nat Neurosci 8:421-5. 2005..Here we show inhibitory coupling between GPCRs and vesicle exocytosis mediated directly by Gbetagamma interactions with the Ca(2+)-dependent fusion machinery... - ADP-ribosylation factor 6 regulation of phosphatidylinositol-4,5-bisphosphate synthesis, endocytosis, and exocytosisYoshikatsu Aikawa
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences at Kagawa, Tokushima-Bunri University, Japan
Methods Enzymol 404:422-31. 2005.... - A second SNARE role for exocytic SNAP25 in endosome fusionYoshikatsu Aikawa
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
Mol Biol Cell 17:2113-24. 2006..Our results indicate that SNAP25 has a second function as an endosomal Q-SNARE in trafficking from the sorting endosome to the recycling endosome and that BoNT E has effects linked to disruption of the endosome recycling pathway... - Role of phosphoinositide signaling in the control of insulin exocytosisLaurent Waselle
Department of Cell Biology and Morphology, Rue du Bugnon 9, 1005 Lausanne, Switzerland
Mol Endocrinol 19:3097-106. 2005....
Research Grants
- CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLSThomas Martin; Fiscal Year: 2006..The results may find application in the therapy of nervous system and endocrine disorders that involve hypo- or hypersecretion of monoamine transmitters or peptide hormones. ..
- STAGES OF REGULATED EXOCYTOSISThomas Martin; Fiscal Year: 2007..__ _ _ i ..
- CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLSThomas Martin; Fiscal Year: 2007..The results from this work will contribute to understanding neural trafficking pathways and to the molecular basis for secretory vesicle fusion with the plasma membrane. ..
- CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLSThomas Martin; Fiscal Year: 2009..The results from this work will contribute to understanding neural trafficking pathways and to the molecular basis for secretory vesicle fusion with the plasma membrane. ..
- STAGES OF REGULATED EXOCYTOSISThomas Martin; Fiscal Year: 2009..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
- CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLSThomas F J Martin; Fiscal Year: 2010..The results from this work will contribute to understanding neural trafficking pathways and to the molecular basis for secretory vesicle fusion with the plasma membrane. ..
- STAGES OF REGULATED EXOCYTOSISThomas F J Martin; Fiscal Year: 2010..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
- STAGES OF REGULATED EXOCYTOSISThomas Martin; Fiscal Year: 2009..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
- CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLSThomas Martin; Fiscal Year: 2001..The goal of specific aim 3 is to characterize the CAPS null phenotype in neural cells. The goal of specific aim 4 is to characterize a new cytosolic factor that may be involved in triggering fusion. ..
- CA2+ REGULATION OF SECRETION IN GH3 PITUITARY CELLSThomas Martin; Fiscal Year: 1992..The outcome of this work will provide direct biochemical characterization of Ca2+-regulated secretion and evaluate the possibility that secretory regulation in several model systems share common features...
- CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLSThomas F J Martin; Fiscal Year: 2010..The results from this work will contribute to understanding neural trafficking pathways and to the molecular basis for secretory vesicle fusion with the plasma membrane. ..