THOMAS F MARTIN

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Phosphatidylinositol 4,5-bisphosphate regulates SNARE-dependent membrane fusion
    Declan J James
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    J Cell Biol 182:355-66. 2008
  2. pmc ARF6 regulates a plasma membrane pool of phosphatidylinositol(4,5)bisphosphate required for regulated exocytosis
    Yoshikatsu Aikawa
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    J Cell Biol 162:647-59. 2003
  3. ncbi request reprint Docked secretory vesicles undergo Ca2+-activated exocytosis in a cell-free system
    T F Martin
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 272:14447-53. 1997
  4. ncbi request reprint Phosphoinositides as spatial regulators of membrane traffic
    T F Martin
    Department of Biochemistry, University of Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706, USA
    Curr Opin Neurobiol 7:331-8. 1997
  5. ncbi request reprint Phosphoinositide lipids as signaling molecules: common themes for signal transduction, cytoskeletal regulation, and membrane trafficking
    T F Martin
    Department of Biochemistry, University of Wisconsin, Madison 53706, USA
    Annu Rev Cell Dev Biol 14:231-64. 1998
  6. ncbi request reprint PI(4,5)P(2) regulation of surface membrane traffic
    T F Martin
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, 53706, Wisconsin, USA
    Curr Opin Cell Biol 13:493-9. 2001
  7. ncbi request reprint Tuning exocytosis for speed: fast and slow modes
    Thomas F J Martin
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    Biochim Biophys Acta 1641:157-65. 2003
  8. ncbi request reprint Prime movers of synaptic vesicle exocytosis
    T F J Martin
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    Neuron 34:9-12. 2002
  9. pmc Synaptotagmin-1 utilizes membrane bending and SNARE binding to drive fusion pore expansion
    Kara L Lynch
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Biol Cell 19:5093-103. 2008
  10. pmc Imaging of evoked dense-core-vesicle exocytosis in hippocampal neurons reveals long latencies and kiss-and-run fusion events
    Xiaofeng Xia
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    J Cell Sci 122:75-82. 2009

Research Grants

  1. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas F J Martin; Fiscal Year: 2010
  2. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas Martin; Fiscal Year: 2006
  3. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2009
  4. STAGES OF REGULATED EXOCYTOSIS
    Thomas F J Martin; Fiscal Year: 2010
  5. CA2+ REGULATION OF SECRETION IN GH3 PITUITARY CELLS
    Thomas Martin; Fiscal Year: 1992
  6. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas Martin; Fiscal Year: 2001
  7. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2009
  8. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas Martin; Fiscal Year: 2009
  9. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas Martin; Fiscal Year: 2007
  10. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2007

Collaborators

Detail Information

Publications27

  1. pmc Phosphatidylinositol 4,5-bisphosphate regulates SNARE-dependent membrane fusion
    Declan J James
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    J Cell Biol 182:355-66. 2008
    ..These results indicate that PI 4,5-P(2) regulates fusion both as a fusion restraint that syntaxin-1 alleviates and as an essential cofactor that recruits protein priming factors to facilitate SNARE-dependent fusion...
  2. pmc ARF6 regulates a plasma membrane pool of phosphatidylinositol(4,5)bisphosphate required for regulated exocytosis
    Yoshikatsu Aikawa
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    J Cell Biol 162:647-59. 2003
    ..We conclude that ARF6 plays a role in Ca2+-dependent DCV exocytosis by regulating the activity of PIP5K for the synthesis of an essential plasma membrane pool of PIP2...
  3. ncbi request reprint Docked secretory vesicles undergo Ca2+-activated exocytosis in a cell-free system
    T F Martin
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 272:14447-53. 1997
    ..A cell-free system for Ca2+-activated fusion will facilitate studies on the roles of essential proteins such as syntaxin, synaptobrevin, SNAP-25, and CAPS that act at post-docking steps in the regulated exocytotic pathway...
  4. ncbi request reprint Phosphoinositides as spatial regulators of membrane traffic
    T F Martin
    Department of Biochemistry, University of Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706, USA
    Curr Opin Neurobiol 7:331-8. 1997
    ..The roles played by phosphoinositides in aspects of secretory granule formation, fusion and endocytosis indicate the importance of phosphorylated lipids for neurotransmitter release...
  5. ncbi request reprint Phosphoinositide lipids as signaling molecules: common themes for signal transduction, cytoskeletal regulation, and membrane trafficking
    T F Martin
    Department of Biochemistry, University of Wisconsin, Madison 53706, USA
    Annu Rev Cell Dev Biol 14:231-64. 1998
    ..Common themes of localized signal generation and the spatially localized recruitment of effector proteins appear to underlie mechanisms employed in signal transduction, cytoskeletal, and membrane trafficking events...
  6. ncbi request reprint PI(4,5)P(2) regulation of surface membrane traffic
    T F Martin
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, 53706, Wisconsin, USA
    Curr Opin Cell Biol 13:493-9. 2001
    ..The targeting of phosphoinositide kinases by GTPases can coordinate the reactions of membrane fusion and fission with cytoskeletal assembly, providing a basis for membrane movement...
  7. ncbi request reprint Tuning exocytosis for speed: fast and slow modes
    Thomas F J Martin
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    Biochim Biophys Acta 1641:157-65. 2003
    ....
  8. ncbi request reprint Prime movers of synaptic vesicle exocytosis
    T F J Martin
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    Neuron 34:9-12. 2002
    ..This work sheds new light on how presynaptic structure provides speed and plasticity to synaptic transmission...
  9. pmc Synaptotagmin-1 utilizes membrane bending and SNARE binding to drive fusion pore expansion
    Kara L Lynch
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Biol Cell 19:5093-103. 2008
    ..The results indicate that Syt-1 uses both Ca(2+)-dependent membrane insertion and SNARE binding to drive fusion pore expansion...
  10. pmc Imaging of evoked dense-core-vesicle exocytosis in hippocampal neurons reveals long latencies and kiss-and-run fusion events
    Xiaofeng Xia
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    J Cell Sci 122:75-82. 2009
    ..Thus, the slow evoked release of neuropeptides could be attributed to very prolonged latencies from stimulation to fusion and transient fusion-pore openings that might limit cargo secretion...
  11. ncbi request reprint Ca2+-dependent synaptotagmin binding to SNAP-25 is essential for Ca2+-triggered exocytosis
    Xiaodong Zhang
    Department of Biochemistry, University of Wisconsin, Madison 53706, USA
    Neuron 34:599-611. 2002
    ..These results indicate that the Ca2+-dependent interaction of synaptotagmin with SNAP-25 is essential for the Ca2+-dependent triggering of membrane fusion...
  12. ncbi request reprint Membrane association domains in Ca2+-dependent activator protein for secretion mediate plasma membrane and dense-core vesicle binding required for Ca2+-dependent exocytosis
    Ruslan N Grishanin
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 277:22025-34. 2002
    ..The presence of two membrane association domains with distinct binding specificities may enable CAPS to bind both target membranes to facilitate DCV-plasma membrane fusion...
  13. ncbi request reprint Different domains of synaptotagmin control the choice between kiss-and-run and full fusion
    Chih Tien Wang
    Department of Physiology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nature 424:943-7. 2003
    ..Syt thus regulates the choice between full fusion and kiss-and-run, with Ca2+ binding to the C2A and C2B domains playing an important role in this choice...
  14. ncbi request reprint CAPS acts at a prefusion step in dense-core vesicle exocytosis as a PIP2 binding protein
    Ruslan N Grishanin
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Neuron 43:551-62. 2004
    ..Regulation of PIP2 levels and CAPS-1 activity would control the secretion of neuropeptides and monoaminergic transmitters...
  15. pmc CAPS activity in priming vesicle exocytosis requires CK2 phosphorylation
    Mari Nojiri
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 284:18707-14. 2009
    ..These results identify a functionally important N-terminal phosphorylation site that regulates CAPS activity in priming vesicle exocytosis...
  16. ncbi request reprint Synaptotagmins I and IX function redundantly in regulated exocytosis but not endocytosis in PC12 cells
    Kara L Lynch
    Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706, USA
    J Cell Sci 120:617-27. 2007
    ..The downregulation of synaptotagmin I but not synaptotagmin IX impaired compensatory vesicle endocytosis, which revealed a lack of isoform redundancy and functional specialization of synaptotagmin I for endocytic retrieval...
  17. pmc Identification of synaptotagmin effectors via acute inhibition of secretion from cracked PC12 cells
    Ward C Tucker
    Department of Physiology, University of Wisconsin, Madison, WI 53706, USA
    J Cell Biol 162:199-209. 2003
    ..These data suggest that syts trigger fusion via their Ca2+-regulated interactions with t-SNAREs and PIP2, target molecules known to play critical roles in exocytosis...
  18. pmc CAPS drives trans-SNARE complex formation and membrane fusion through syntaxin interactions
    Declan J James
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 106:17308-13. 2009
    ..The results revealed an unexpected activity of a priming protein to accelerate fusion by efficiently promoting trans-SNARE complex formation. CAPS may function in priming by organizing SNARE complexes on the plasma membrane...
  19. pmc SNAP25, but not syntaxin 1A, recycles via an ARF6-regulated pathway in neuroendocrine cells
    Yoshikatsu Aikawa
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Biol Cell 17:711-22. 2006
    ..Our results characterize a robust ARF6-dependent internalization mechanism that maintains an intracellular pool of SNAP25, which is compatible with possible intracellular roles for SNAP25 in neuroendocrine cells...
  20. ncbi request reprint Role of CAPS in dense-core vesicle exocytosis
    James J Wassenberg
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Ann N Y Acad Sci 971:201-9. 2002
    ..The results are consistent with a widespread functional role of CAPS in the regulated exocytosis of DCVs in the nervous and endocrine systems...
  21. ncbi request reprint A family of Ca2+-dependent activator proteins for secretion: comparative analysis of structure, expression, localization, and function
    Dina Speidel
    Department of Molecular Neurobiology, Max Planck Institute for Experimental Medicine and Deutsche Forschungsgemeinschaft Center for Molecular Physiology of the Brain, D 37075 Gottingen, Germany
    J Biol Chem 278:52802-9. 2003
    ..The abundance of CAPS proteins in synaptic terminals indicates that they may also be important for neuronal functions that are not exclusively related to LDCV exocytosis...
  22. ncbi request reprint Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells
    Mitsunori Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:4601-4. 2002
    ..Our results support the idea that Syt family proteins that co-reside on secretory vesicles may function cooperatively and redundantly as potential Ca(2+) sensors for exocytosis...
  23. ncbi request reprint UNC-31 (CAPS) is required for dense-core vesicle but not synaptic vesicle exocytosis in Caenorhabditis elegans
    Sean Speese
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    J Neurosci 27:6150-62. 2007
    ..Our results suggest that CAPS/UNC-31 and UNC-13 serve parallel and dedicated roles in dense-core vesicle and synaptic vesicle exocytosis, respectively, in the C. elegans nervous system...
  24. ncbi request reprint G protein betagamma directly regulates SNARE protein fusion machinery for secretory granule exocytosis
    Trillium Blackmer
    Department of Biological Sciences, University of Illinois at Chicago, 840 West Taylor Street, Chicago, Illinois 60607, USA
    Nat Neurosci 8:421-5. 2005
    ..Here we show inhibitory coupling between GPCRs and vesicle exocytosis mediated directly by Gbetagamma interactions with the Ca(2+)-dependent fusion machinery...
  25. ncbi request reprint ADP-ribosylation factor 6 regulation of phosphatidylinositol-4,5-bisphosphate synthesis, endocytosis, and exocytosis
    Yoshikatsu Aikawa
    Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences at Kagawa, Tokushima Bunri University, Japan
    Methods Enzymol 404:422-31. 2005
    ....
  26. pmc A second SNARE role for exocytic SNAP25 in endosome fusion
    Yoshikatsu Aikawa
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Biol Cell 17:2113-24. 2006
    ..Our results indicate that SNAP25 has a second function as an endosomal Q-SNARE in trafficking from the sorting endosome to the recycling endosome and that BoNT E has effects linked to disruption of the endosome recycling pathway...
  27. ncbi request reprint Role of phosphoinositide signaling in the control of insulin exocytosis
    Laurent Waselle
    Department of Cell Biology and Morphology, Rue du Bugnon 9, 1005 Lausanne, Switzerland
    Mol Endocrinol 19:3097-106. 2005
    ....

Research Grants30

  1. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas F J Martin; Fiscal Year: 2010
    ..The results from this work will contribute to understanding neural trafficking pathways and to the molecular basis for secretory vesicle fusion with the plasma membrane. ..
  2. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas Martin; Fiscal Year: 2006
    ..The results may find application in the therapy of nervous system and endocrine disorders that involve hypo- or hypersecretion of monoamine transmitters or peptide hormones. ..
  3. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2009
    ..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
  4. STAGES OF REGULATED EXOCYTOSIS
    Thomas F J Martin; Fiscal Year: 2010
    ..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
  5. CA2+ REGULATION OF SECRETION IN GH3 PITUITARY CELLS
    Thomas Martin; Fiscal Year: 1992
    ..The outcome of this work will provide direct biochemical characterization of Ca2+-regulated secretion and evaluate the possibility that secretory regulation in several model systems share common features...
  6. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas Martin; Fiscal Year: 2001
    ..The goal of specific aim 3 is to characterize the CAPS null phenotype in neural cells. The goal of specific aim 4 is to characterize a new cytosolic factor that may be involved in triggering fusion. ..
  7. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2009
    ..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
  8. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas Martin; Fiscal Year: 2009
    ..The results from this work will contribute to understanding neural trafficking pathways and to the molecular basis for secretory vesicle fusion with the plasma membrane. ..
  9. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas Martin; Fiscal Year: 2007
    ..The results from this work will contribute to understanding neural trafficking pathways and to the molecular basis for secretory vesicle fusion with the plasma membrane. ..
  10. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2007
    ..__ _ _ i ..
  11. CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS
    Thomas F J Martin; Fiscal Year: 2010
    ..The results from this work will contribute to understanding neural trafficking pathways and to the molecular basis for secretory vesicle fusion with the plasma membrane. ..