Research Topics
| Wallace MarshallSummaryAffiliation: University of California Country: USA Publications
Research Grants
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Detail Information
Publications
Centriole asymmetry determines algal cell geometryWallace F Marshall
Department of Biochemistry and Biophysics, UCSF, San Francisco, CA 94158, United States
Curr Opin Plant Biol 15:632-7. 2012..Thus to understand cell organization, it will be critical to understand how the different triplets of the centriole come to have distinct molecular identities...
Organelle size control systems: From cell geometry to organelle-directed medicineWallace F Marshall
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA
Bioessays 34:721-4. 2012..Organelle size is tuned to metabolic needs, hence reprogramming organelle size could be a novel therapeutic strategy as well as a new tool for metabolic engineering...
Quantitative high-throughput assays for flagella-based motility in chlamydomonas using plate-well image analysis and transmission correlation spectroscopyWallace F Marshall
Department of Biochemistry and Biophysics, University of California, San Francisco 94158, USA
J Biomol Screen 14:133-41. 2009..The 2 assays have different advantages in terms of speed and sensitivity to small reductions in motility and may be most efficiently used in combination...- Cilia self-organize in response to planar cell polarity and flowWallace F Marshall
Department of Biochemistry and Biophysics, UCSF, San Francisco, CA 94158, USA
Nat Cell Biol 12:314-5. 2010..We now learn how asymmetric localization of PCP proteins influences the position and orientation of cilia to control the direction of flow... - Centrosome size: scaling without measuringWallace F Marshall
Dept of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA
Curr Biol 21:R594-6. 2011..This simple scheme explains size control and scaling of centrosomes relative to cell volume... - Origins of cellular geometryWallace F Marshall
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 2200, USA
BMC Biol 9:57. 2011.... - The mother centriole plays an instructive role in defining cell geometryJessica L Feldman
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
PLoS Biol 5:e149. 2007..The genes documented here that are required for proper centriole positioning may represent a new class of ciliary disease genes, defects in which would be expected to cause disorganized ciliary position and impaired function... - Cilia orientation and the fluid mechanics of developmentWallace F Marshall
Department of Biochemistry and Biophysics, UCSF, San Fransciso, CA 94158, USA
Curr Opin Cell Biol 20:48-52. 2008..Errors in cilia orientation have been observed in human ciliary disease patients, suggesting that orientation defects may constitute a novel class of ciliopathies with a distinct etiology at the cell biological level... - Isolation and proteomic analysis of Chlamydomonas centriolesLani C Keller
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA
Methods Mol Biol 432:289-300. 2008..Isolated centrioles can be used in combination with a wide variety of biochemical assays in addition to being utilized as a source for proteomic analysis... - Building the cell: design principles of cellular architectureSusanne M Rafelski
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94158, USA
Nat Rev Mol Cell Biol 9:593-602. 2008..By examining how these parameters are controlled in specific cell biological examples we can identify a handful of simple design principles that seem to underlie cellular architecture and assembly... - Engineering design principles for organelle size control systemsWallace F Marshall
Department of Biochemistry and Biophysics, University of California San Francisco, United States
Semin Cell Dev Biol 19:520-4. 2008..Each class represents a design principle by which artificial size controllers could be developed for synthetic biology applications... - Modeling recursive RNA interferenceWallace F Marshall
Department of Biochemistry and Biophysics, Integrative Program in Quantitative Biology, University of California San Francisco, San Francisco, California, United States of America
PLoS Comput Biol 4:e1000183. 2008..The model suggests potential ways to optimize the effectiveness of recursive RNAi both for screening of RNAi components as well as for improved temporal control of gene expression in switch off-switch on experiments... - Molecular architecture of the centriole proteome: the conserved WD40 domain protein POC1 is required for centriole duplication and length controlLani C Keller
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
Mol Biol Cell 20:1150-66. 2009..Together, these data suggest that POC1 is involved in early steps of centriole duplication as well as in the later steps of centriole length control... - Centriole evolutionWallace F Marshall
Dept of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA
Curr Opin Cell Biol 21:14-9. 2009..Far from being irreducibly complex, centrioles appear to be based on a rather minimal underlying core structure requiring only a handful of genes to construct... - ASQ2 encodes a TBCC-like protein required for mother-daughter centriole linkage and mitotic spindle orientationJessica L Feldman
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
Curr Biol 19:1238-43. 2009..The defects in centriole number control, centriole positioning, and spindle orientation appear to arise from perturbation of centriole linkage mediated by Tbccd1/Asq2p... - Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagellaBenjamin D Engel
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94158, USA
Methods Cell Biol 93:157-77. 2009..This chapter also reviews current IFT insights gleaned from TIRF microscopy of Chlamydomonas and provides an outlook on the future of the technique, with particular emphasis on combining TIRF with other emerging imaging technologies... - The cell biological basis of ciliary diseaseWallace F Marshall
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
J Cell Biol 180:17-21. 2008..The clinical complexity of the ciliopathies can therefore only be understood in light of the basic cell biology of the cilia themselves, which I will discuss from the viewpoint of cell biological studies in model organisms... - Centriole assembly: the origin of nine-nessWallace F Marshall
Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th St, San Francisco, California, USA
Curr Biol 17:R1057-9. 2007..Recent studies of the Chlamydomonas bld10 mutant have revealed that the ninefold symmetry of the centriole is set by the length of the cartwheel spokes, which fixes the diameter, and thereby the circumference, of the centriole... - Centrioles: bad to be bald?Jessica L Feldman
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143, USA
Curr Biol 14:R659-60. 2004..In the alga Chlamydomonas, mutation of the gene encoding the novel centriolar component Bld10p results in seemingly acentriolar cells. Remarkably, bld10 cells are viable, highlighting the question of whether or not centrioles are essential... - Cellular length control systemsWallace F Marshall
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA
Annu Rev Cell Dev Biol 20:677-93. 2004..The lessons learned from size control in linear structures should be applicable to organelles with more complex three-dimensional structures... - Flagellar length control system: testing a simple model based on intraflagellar transport and turnoverWallace F Marshall
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
Mol Biol Cell 16:270-8. 2005..These results are consistent with the balance-point model for length control. The three strategies used here are not limited to flagella and can in principle be adapted to probe size control systems for any organelle... - A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtiiIvan Zamora
Dept of Biochemistry and Biophysics, University of California San Francisco, 600 16th St, San Francisco, California, USA
BMC Biol 3:15. 2005..The role of centrioles in mitotic spindle function remains unclear. One approach to investigate mitotic centriole function is to ask whether mutation of centriole-associated proteins can cause genomic instability... - Gene expression and nuclear architecture during development and differentiationWallace F Marshall
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
Mech Dev 120:1217-30. 2003..The remainder of the review will examine developmental processes from the point of view of the nucleus... - De novo formation of left-right asymmetry by posterior tilt of nodal ciliaShigenori Nonaka
Graduate School of Frontier Biosciences, Osaka University, Japan
PLoS Biol 3:e268. 2005.... - Cilia: tuning in to the cell's antennaWallace F Marshall
Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th St, San Francisco, California 94143, USA
Curr Biol 16:R604-14. 2006..While these diseases highlight the pivotal roles of cilia in physiology and development, the mechanistic link between cilia, physiology, and disease remains unclear... - Don'T blink: observing the ultra-fast contraction of spasmonemesWallace F Marshall
Department of Biochemistry and Biophysics Integrative Program in Quantitative Biology, University of California at San Francisco, San Francisco, California 94158, USA
Biophys J 94:4-5. 2008 - What is the function of centrioles?Wallace F Marshall
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA
J Cell Biochem 100:916-22. 2007.... - Stability and robustness of an organelle number control system: modeling and measuring homeostatic regulation of centriole abundanceWallace F Marshall
Department of Biochemistry and Biophysics and Integrative Program in Quantitative Biology, University of California, San Francisco, CA, USA
Biophys J 93:1818-33. 2007.... - Flagellar length control in chlamydomonas--paradigm for organelle size regulationKimberly A Wemmer
Department of Biochemistry, University of California, San Francisco, California 94158, USA
Int Rev Cytol 260:175-212. 2007..Studies in this organism using genetics, biochemistry, imaging, and mathematical modeling have revealed many genes involved in length control of cilia and flagella, and have suggested several testable models for length regulation... - Basal bodies platforms for building ciliaWallace F Marshall
Department of Biochemistry and Biophysics, University of California, San Francisco, California, USA
Curr Top Dev Biol 85:1-22. 2008..The "parts list" of basal body proteins and genes is rapidly being completed, opening the way to more mechanistic studies in the future... - Influence of centriole number on mitotic spindle length and symmetryLani C Keller
Department of Biochemistry and Biophysics, UCSF, San Francisco, California 94158, USA
Cytoskeleton (Hoboken) 67:504-18. 2010.... - Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genesLani C Keller
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
Curr Biol 15:1090-8. 2005..CONCLUSIONS: By analyzing isolated Chlamydomonas basal bodies, we have been able to obtain the first reported proteomic analysis of the centriole... - Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control modelBenjamin D Engel
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
J Cell Biol 187:81-9. 2009.... - Flagellar motility: all pull togetherKimberly A Wemmer
Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, San Francisco, California 94143, USA
Curr Biol 14:R992-3. 2004..Eukaryotic flagella produce a swimming force by coordinating thousands of dynein motor proteins. Recent work provides new clues into how this coordination is achieved... - Use of transcriptomic data to support organelle proteomic analysisWallace F Marshall
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA
Methods Mol Biol 432:403-14. 2008..Such an approach can be used to narrow down a preliminary proteomic data set and help us to focus on a smaller sub-set of proteins that are supported by transcriptomic cross-validation... - Quantitative modeling in cell biology: what is it good for?Alex Mogilner
Department of Neurobiology, Physiology and Behavior, University of California, Davis, 95616, USA
Dev Cell 11:279-87. 2006.... - PCR-based assay for mating type and diploidy in ChlamydomonasIvan Zamora
University of California, San Francisco, CA 94143, USA
Biotechniques 37:534-6. 2004 - A cell-based screen for inhibitors of flagella-driven motility in Chlamydomonas reveals a novel modulator of ciliary length and retrograde actin flowBenjamin D Engel
Department of Biochemistry and Biophysics, University of California, San Francisco, USA
Cytoskeleton (Hoboken) 68:188-203. 2011....
Research Grants
- Genetic Analysis of Centriole Duplication and FunctionWallace Marshall; Fiscal Year: 2007..These mutants, as well as mutants altering centriole ultrastructure, will be used to test the role of centrioles in chromosome segregation, mitotic spindle organization, nuclear positioning, and cytokinesis. ..
- Genetic Analysis of Centriole Duplication and FunctionWallace Marshall; Fiscal Year: 2010..These mutants, as well as mutants altering centriole ultrastructure, will be used to test the role of centrioles in chromosome segregation, mitotic spindle organization, nuclear positioning, and cytokinesis. ..
- Pattern formation & regeneration in a single cellWallace Marshall; Fiscal Year: 2010..We propose an alternative approach, which is to induce the injured cells to repair themselves. Towards this end, we will study a classical single-celled organism that has the ability to regenerate itself following damage. ..