R Marmorstein

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi request reprint Structure of SET domain proteins: a new twist on histone methylation
    Ronen Marmorstein
    The Wistar Institute, The Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Trends Biochem Sci 28:59-62. 2003
  2. pmc Structural basis for nicotinamide inhibition and base exchange in Sir2 enzymes
    Brandi D Sanders
    The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Cell 25:463-72. 2007
  3. pmc Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme
    Keqin Li
    The Wistar Institute University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 372:194-204. 2007
  4. pmc Structural basis for DNA recognition by FoxO1 and its regulation by posttranslational modification
    Michael M Brent
    The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA The Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Structure 16:1407-16. 2008
  5. ncbi request reprint Protein modules that manipulate histone tails for chromatin regulation
    R Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Rev Mol Cell Biol 2:422-32. 2001
  6. ncbi request reprint Structure and chemistry of the Sir2 family of NAD+-dependent histone/protein deactylases
    R Marmorstein
    The Wistar Institute and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Biochem Soc Trans 32:904-9. 2004
  7. ncbi request reprint Modulation of DNA-binding domains for sequence-specific DNA recognition
    Ronen Marmorstein
    Department of Chemistry, The Wistar Institute, University of Pennsylvania, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Gene 304:1-12. 2003
  8. ncbi request reprint Structure and function of histone acetyltransferases
    R Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia 19104, USA
    Cell Mol Life Sci 58:693-703. 2001
  9. ncbi request reprint Structure of histone deacetylases: insights into substrate recognition and catalysis
    R Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Structure 9:1127-33. 2001
  10. ncbi request reprint Structure of histone acetyltransferases
    R Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 311:433-44. 2001

Research Grants

  1. Predoctoral Training at the Chemistry-Biology Interface
    Ronen Marmorstein; Fiscal Year: 2007
  2. TRAINING PROGRAM IN BASIC CANCER RESEARCH
    Ronen Marmorstein; Fiscal Year: 2007

Collaborators

Detail Information

Publications75

  1. ncbi request reprint Structure of SET domain proteins: a new twist on histone methylation
    Ronen Marmorstein
    The Wistar Institute, The Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Trends Biochem Sci 28:59-62. 2003
    ....
  2. pmc Structural basis for nicotinamide inhibition and base exchange in Sir2 enzymes
    Brandi D Sanders
    The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Cell 25:463-72. 2007
    ..These results provide insights into the Sir2 mechanism of nicotinamide inhibition and have important implications for the development of Sir2-specific effectors...
  3. pmc Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme
    Keqin Li
    The Wistar Institute University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 372:194-204. 2007
    ..Together, these studies provide new insights into Ubp3 recognition by Bre5 and into protein recognition by NTF2-like domains...
  4. pmc Structural basis for DNA recognition by FoxO1 and its regulation by posttranslational modification
    Michael M Brent
    The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA The Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Structure 16:1407-16. 2008
    ....
  5. ncbi request reprint Protein modules that manipulate histone tails for chromatin regulation
    R Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Rev Mol Cell Biol 2:422-32. 2001
    ..It is becoming clear that appropriate coordination of histone modifications and their manipulations by conserved protein modules are integral to gene-specific transcriptional regulation within chromatin...
  6. ncbi request reprint Structure and chemistry of the Sir2 family of NAD+-dependent histone/protein deactylases
    R Marmorstein
    The Wistar Institute and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Biochem Soc Trans 32:904-9. 2004
    ..These studies have implications for the structure-based design of Sir2-specific small molecule compounds, which might modulate Sir2 function for therapeutic application...
  7. ncbi request reprint Modulation of DNA-binding domains for sequence-specific DNA recognition
    Ronen Marmorstein
    Department of Chemistry, The Wistar Institute, University of Pennsylvania, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Gene 304:1-12. 2003
    ..Together, these examples reveal a variety of ways in which such extrinsic factors can significantly extend the repertoire of DNA sites recognized by a given DNA-binding domain...
  8. ncbi request reprint Structure and function of histone acetyltransferases
    R Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia 19104, USA
    Cell Mol Life Sci 58:693-703. 2001
    ....
  9. ncbi request reprint Structure of histone deacetylases: insights into substrate recognition and catalysis
    R Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Structure 9:1127-33. 2001
    ..Recent structural studies of histone deacetylases and their homologs from bacteria have provided important insights into the mode of substrate recognition and catalysis by these enzymes...
  10. ncbi request reprint Structure of histone acetyltransferases
    R Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 311:433-44. 2001
    ..These correlations imply a related mode of catalysis and histone substrate binding by a diverse group of HAT enzymes...
  11. ncbi request reprint Histone acetyltransferases: function, structure, and catalysis
    R Marmorstein
    Structural Biology Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Curr Opin Genet Dev 11:155-61. 2001
    ..Developmental aberrations in mice and certain human cancers are associated with HAT mutations, further highlighting the importance of these enzymes to normal cell growth and differentiation...
  12. ncbi request reprint Crystal structure of a ternary SAP-1/SRF/c-fos SRE DNA complex
    Y Mo
    The Wistar Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Mol Biol 314:495-506. 2001
    ....
  13. ncbi request reprint Structure of the elk-1-DNA complex reveals how DNA-distal residues affect ETS domain recognition of DNA
    Y Mo
    The Wistar Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Struct Biol 7:292-7. 2000
    ....
  14. ncbi request reprint Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14
    W S Lo
    Molecular Genetics Program, Wistar Institute, Philadelphia, Pennsylvania 19024, USA
    Mol Cell 5:917-26. 2000
    ..These observations suggest that transcriptional regulation occurs by multiple mechanistically linked covalent modifications of histones...
  15. ncbi request reprint Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins
    Y Mo
    The Wistar Institute, Department of Chemistry, University of Pennsylvania, Philadelphia 19104, USA
    Mol Cell 2:201-12. 1998
    ..Modeling studies of a SAP-1/SRF/DNA complex suggest that SRF may modulate SAP-1 binding to DNA by interacting with its ETS domain...
  16. pmc p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage
    L Liu
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 19:1202-9. 1999
    ..These data indicate that site-specific acetylation of p53 increases under physiological conditions that activate p53 and identify CBP/p300 and PCAF as the probable enzymes that modify p53 in vivo...
  17. ncbi request reprint Crystal structure of the CDK4/6 inhibitory protein p18INK4c provides insights into ankyrin-like repeat structure/function and tumor-derived p16INK4 mutations
    R Venkataramani
    Wistar Institute, Philadelphia, Pennsylvania, USA
    Nat Struct Biol 5:74-81. 1998
    ..A subset of these residues suggest an INK4 binding surface for the cyclin-dependent kinases 4 and 6. This surface is centered around a region that shows structural features uncharacteristic of ankyrin-like repeat units...
  18. ncbi request reprint Structure and function of bromodomains in chromatin-regulating complexes
    R Marmorstein
    Structural Biology Program, The Wistar Institute, Philadelphia, PA, USA
    Gene 272:1-9. 2001
    ..Interactions between bromodomains and modified histones may be an important mechanism underlying chromatin structural changes and gene regulation...
  19. ncbi request reprint Structure-function studies of the BTB/POZ transcriptional repression domain from the promyelocytic leukemia zinc finger oncoprotein
    X Li
    The Wistar Institute, University of Pennsylvania, Philadelphia 19104, USA
    Cancer Res 59:5275-82. 1999
    ..Together, these studies form a structure-function framework for understanding BTB/POZ-mediated oligomerization and transcriptional repression properties...
  20. ncbi request reprint Crystal structure of the mouse p53 core DNA-binding domain at 2.7 A resolution
    K Zhao
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, 19104, USA
    J Biol Chem 276:12120-7. 2001
    ..We propose that the p53 core domain dimer that is seen in the crystals described here represents a physiologically relevant inactive form of p53 that must undergo structural rearrangement for sequence-specific DNA binding...
  21. ncbi request reprint Chemistry of acetyl transfer by histone modifying enzymes: structure, mechanism and implications for effector design
    S C Hodawadekar
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Oncogene 26:5528-40. 2007
    ..In this review, we will discuss what we have learned from the structures and related biochemistry of HATs and HDACs and the implications of these findings for the design of protein effectors to regulate gene expression and treat disease...
  22. pmc Crystal structure of a p53 core tetramer bound to DNA
    K A Malecka
    The Wistar Institute, Philadelphia, PA 19104, USA
    Oncogene 28:325-33. 2009
    ..Surface residue conservation of the p53DBD tetramer bound to DNA highlights possible regions of other p53 domain or p53 cofactor interactions...
  23. ncbi request reprint Structure and substrate binding properties of cobB, a Sir2 homolog protein deacetylase from Escherichia coli
    Kehao Zhao
    The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 337:731-41. 2004
    ..Together, these studies suggest that substrate-specific binding by sirtuin proteins involves contributions from the zinc-binding domain of the enzyme and substrate regions distal to the acetyl-lysine-binding site...
  24. ncbi request reprint Structure of the yeast Hst2 protein deacetylase in ternary complex with 2'-O-acetyl ADP ribose and histone peptide
    Kehao Zhao
    The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Structure 11:1403-11. 2003
    ....
  25. pmc Structure and biochemical characterization of protein acetyltransferase from Sulfolobus solfataricus
    Michael M Brent
    Wistar Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 284:19412-9. 2009
    ..The structural similarity of PAT to eukaryotic HATs combined with its conserved role in chromatin regulation suggests that PAT is evolutionarily related to the eukaryotic HATs...
  26. ncbi request reprint Structure of a Leu3-DNA complex: recognition of everted CGG half-sites by a Zn2Cys6 binuclear cluster protein
    Mary X Fitzgerald
    The Wistar Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Structure 14:725-35. 2006
    ..Broader implications of these findings are discussed...
  27. ncbi request reprint Crystal structure of a PUT3-DNA complex reveals a novel mechanism for DNA recognition by a protein containing a Zn2Cys6 binuclear cluster
    K Swaminathan
    Wistar Institute, University of Pennsylvania, Philadelphia 19104, USA
    Nat Struct Biol 4:751-9. 1997
    ..A comparison with the GAL4-DNA and PPR1-DNA complexes shows how a family of related DNA binding proteins can use a diverse set of mechanisms to discriminate between the base pairs separating conserved DNA half-sites...
  28. ncbi request reprint Structural basis for interaction between the Ubp3 deubiquitinating enzyme and its Bre5 cofactor
    Keqin Li
    The Wistar Institute, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Biol Chem 280:29176-85. 2005
    ..Together, these studies provide novel insights into protein recognition by NTF2-like domains and provide a molecular scaffold for understanding how Ubp3 function is regulated by Bre5 cofactor binding...
  29. ncbi request reprint Structure of a Sir2 substrate, Alba, reveals a mechanism for deacetylation-induced enhancement of DNA binding
    Kehao Zhao
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 278:26071-7. 2003
    ..Implications for the mechanism by which histone acetylation modulates gene expression are discussed...
  30. ncbi request reprint Generation and characterization of monoclonal antibodies against the E6 and E7 oncoproteins of HPV
    A P Wlazlo
    The Wistar Institute, Philadelphia, PA 19104, USA
    Hybridoma 20:257-63. 2001
    ..The E7 MAb cross-reacted with the HPV-1a E7 oncoprotein. The binding sites of the MAbs were mapped to defined regions of each viral protein...
  31. pmc Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes
    Guoping Da
    The Wistar Institute and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 103:2057-62. 2006
    ..Together, these studies identify the SWIRM domain as an essential multifunctional module for the regulation of gene expression...
  32. ncbi request reprint Structure of the p53 core domain dimer bound to DNA
    William C Ho
    The Wistar Institute, Pennsylvania 19104, USA
    J Biol Chem 281:20494-502. 2006
    ....
  33. pmc Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells
    Gowrishankar Banumathy
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Mol Cell Biol 29:758-70. 2009
    ..UBN1 is indispensable for formation of SAHF. We conclude that UBN1 is an ortholog of yeast Hpc2p and a novel regulator of senescence...
  34. ncbi request reprint The human monocytic leukemia zinc finger histone acetyltransferase domain contains DNA-binding activity implicated in chromatin targeting
    Marc A Holbert
    The Wistar Institute and Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 0381, USA
    J Biol Chem 282:36603-13. 2007
    ..Our results reveal, for the first time, that enzymatic and DNA-targeting activities can be contained within the same chromatin regulatory domain...
  35. pmc Fungal Rtt109 histone acetyltransferase is an unexpected structural homolog of metazoan p300/CBP
    Yong Tang
    Program in Gene Expression and Regulation, The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nat Struct Mol Biol 15:738-45. 2008
    ..The structure reveals a buried autoacetylated lysine residue that we show is also acetylated in the Rtt109 protein purified from yeast cells. Implications for understanding histone substrate and chaperone binding by Rtt109 are discussed...
  36. ncbi request reprint Molecular basis for Gcn5/PCAF histone acetyltransferase selectivity for histone and nonhistone substrates
    Arienne N Poux
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 42:14366-74. 2003
    ..Together, these studies provide a framework for understanding the substrate selectivity of HAT proteins...
  37. pmc Structural basis for dimerization in DNA recognition by Gal4
    Manqing Hong
    The Wistar Institute, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Structure 16:1019-26. 2008
    ..Associated biochemical studies show that the dimerization domain of Gal4 is important for DNA binding and protein thermostability. We also map the interaction surface of the Gal4 dimerization domain with Gal11P...
  38. ncbi request reprint Structural basis for histone and phosphohistone binding by the GCN5 histone acetyltransferase
    Adrienne Clements
    The Wistar Institute, Philadelphia, PA 19104, USA
    Mol Cell 12:461-73. 2003
    ..Together, these studies reveal how one histone modification can modulate another to affect distinct transcriptional signals...
  39. ncbi request reprint The structurally disordered KRAB repression domain is incorporated into a protease resistant core upon binding to KAP-1-RBCC domain
    Hongzhuang Peng
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Mol Biol 370:269-89. 2007
    ..This is the first example of a structurally disordered repressor domain that is the most widely conserved silencing domain in tetrapods...
  40. pmc Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly
    Yong Tang
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Nat Struct Mol Biol 13:921-9. 2006
    ..Together, these studies begin to define the molecular determinants of assembly of functionally diverse macromolecular histone chaperone complexes...
  41. pmc Structure of Vps75 and implications for histone chaperone function
    Yong Tang
    Program in Gene Expression and Regulation, Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 105:12206-11. 2008
    ..Taken together, our data provide a structural framework for understanding how Vps75 mediates both nucleosome assembly and histone acetylation by Rtt109...
  42. ncbi request reprint Dehydrogenases, NAD, and transcription--what's the connection?
    Ronen Marmorstein
    The Wistar Institute and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Structure 10:1465-6. 2002
    ....
  43. ncbi request reprint Structure and autoregulation of the yeast Hst2 homolog of Sir2
    Kehao Zhao
    The Wistar Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Struct Biol 10:864-71. 2003
    ..Together, these studies indicate that the sequence-divergent N- and C-terminal regions of the eukaryotic Sir2 proteins may have a particularly important role in their distinct substrate-binding properties, biological activities or both...
  44. ncbi request reprint Transcription initiation at its most basic level
    Ronen Marmorstein
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cell 115:370-2. 2003
    ....
  45. ncbi request reprint High-resolution structure of the p53 core domain: implications for binding small-molecule stabilizing compounds
    William C Ho
    The Wistar Institute, Philadelphia, PA 19104, USA
    Acta Crystallogr D Biol Crystallogr 62:1484-93. 2006
    ..Together, these studies provide a molecular scaffold for the structure-based design of p53-stabilization compounds for development as possible therapeutic agents...
  46. ncbi request reprint Insights into structure and function of GCN5/PCAF and yEsa 1 histone acetyltransferase domains:
    Adrienne Clements
    Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Methods Enzymol 371:545-64. 2003
  47. ncbi request reprint Structural and chemical basis of histone acetylation
    Ronen Marmorstein
    The Wistar Institute, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Novartis Found Symp 259:78-98; discussion 98-101, 163-9. 2004
    ..In this paper I will review structural work from my laboratory on histone acetyltransferases (HATs) and the Sir2 family of histone deacetylases (HDACs), with a specific focus on catalysis and substrate-specific binding by these enzymes...
  48. ncbi request reprint Maintenance of low histone ubiquitylation by Ubp10 correlates with telomere-proximal Sir2 association and gene silencing
    N C Tolga Emre
    Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, PA 19024, USA
    Mol Cell 17:585-94. 2005
    ..Our results suggest that these H2B-deubiquitylating enzymes have distinct genomic functions...
  49. pmc Structure of the retinoblastoma protein bound to adenovirus E1A reveals the molecular basis for viral oncoprotein inactivation of a tumor suppressor
    Xin Liu
    Program in Gene Expression and Regulation, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 21:2711-6. 2007
    ....
  50. pmc Structural basis for ubiquitin recognition by the Otu1 ovarian tumor domain protein
    Troy Eugene Messick
    Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 283:11038-49. 2008
    ..Together, these studies provide new insights into ubiquitin binding and hydrolysis by yeast Otu1 and other OTU domain-containing proteins...
  51. doi request reprint The structural basis of protein acetylation by the p300/CBP transcriptional coactivator
    Xin Liu
    Program in Gene Expression and Regulation, The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 451:846-50. 2008
    ..Several disease-associated mutations can also be readily accounted for by the p300 HAT structure. These studies pave the way for new epigenetic therapies involving modulation of p300/CBP HAT activity...
  52. pmc Biochemical analysis of MST1 kinase: elucidation of a C-terminal regulatory region
    Ruchi Anand
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 47:6719-26. 2008
    ..Together, our studies provide new insights into how MST1 substrate selectivity is modulated with implications for understanding apoptotic signaling through MST1 kinase...
  53. ncbi request reprint Biochemical and structural characterization of recombinant histone acetyltransferase proteins
    Ronen Marmorstein
    Structural Biology Program, The Wistar Institute, Philadelphia, Pennsylvania 19104 4268, USA
    Methods Enzymol 376:106-19. 2004
  54. ncbi request reprint Structure and mechanism of lysine-specific demethylase enzymes
    Ruchi Anand
    Program in Gene Expression and Regulation, The Wistar Institute, Philadelphia, Pennsylvania, USA
    J Biol Chem 282:35425-9. 2007
    ....
  55. pmc Structure of the GCN5 histone acetyltransferase bound to a bisubstrate inhibitor
    Arienne N Poux
    The Wistar Institute, and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 99:14065-70. 2002
    ..The structure also provides a structural scaffold for the design of HAT-specific inhibitors that may have therapeutic applications for the treatment of HAT-mediated cancers...
  56. ncbi request reprint Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family
    Vinod Nayak
    The Wistar Institute, University of Pennsylvania, Philadelphia 19104, USA
    Structure 14:477-85. 2006
    ..Taken together, these studies suggest another layer of kinase regulation within the Snf1/AMPK family, and an avenue for development of AMPK-specific activating compounds...
  57. ncbi request reprint Structure and activity of enzymes that remove histone modifications
    Marc A Holbert
    The Wistar Institute and the Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Opin Struct Biol 15:673-80. 2005
    ....
  58. ncbi request reprint Structure of the human Papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor
    Xin Liu
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 281:578-86. 2006
    ....
  59. pmc Structural basis for nicotinamide cleavage and ADP-ribose transfer by NAD(+)-dependent Sir2 histone/protein deacetylases
    Kehao Zhao
    The Wistar Institute, Department of Biochemistry and Biophysics, School of Medicine, and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104
    Proc Natl Acad Sci U S A 101:8563-8. 2004
    ..Together, these studies provide insights into the chemistry of NAD(+) cleavage and acetylation by Sir2 proteins and have implications for the design of Sir2-specific regulatory molecules...
  60. pmc Histone modifying enzymes: structures, mechanisms, and specificities
    Ronen Marmorstein
    Program in Gene Expression and Regulation, The Wistar Institute, Philadelphia, PA 19104, USA
    Biochim Biophys Acta 1789:58-68. 2009
    ....
  61. ncbi request reprint The catalytic mechanism of the ESA1 histone acetyltransferase involves a self-acetylated intermediate
    Yuan Yan
    The Wistar Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Struct Biol 9:862-9. 2002
    ..These results demonstrate that different HAT subfamilies can use distinct catalytic mechanisms, which have implications for their distinct biological roles and for the development of HAT-specific inhibitors...
  62. pmc Structure-based design of an organoruthenium phosphatidyl-inositol-3-kinase inhibitor reveals a switch governing lipid kinase potency and selectivity
    Peng Xie
    Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    ACS Chem Biol 3:305-16. 2008
    ....
  63. pmc Targeting large kinase active site with rigid, bulky octahedral ruthenium complexes
    Jasna Maksimoska
    The Wistar Institute and University of Pennsylvania, Department of Chemistry, Philadelphia, Pennsylvania 19104, USA
    J Am Chem Soc 130:15764-5. 2008
    ....
  64. pmc Acetylation of the p53 DNA-binding domain regulates apoptosis induction
    Stephen M Sykes
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Mol Cell 24:841-51. 2006
    ..Furthermore, the acetyllysine 120 (acetyl-K120) form of p53 specifically accumulates at proapoptotic target genes. These data suggest that K120 acetylation may help distinguish the cell-cycle arrest and apoptotic functions of p53...
  65. pmc Toward the development of a potent and selective organoruthenium mammalian sterile 20 kinase inhibitor
    Ruchi Anand
    Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    J Med Chem 52:1602-11. 2009
    ....
  66. pmc Structural basis for sirtuin function: what we know and what we don't
    Brandi D Sanders
    The Wistar Institute and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Biochim Biophys Acta 1804:1604-16. 2010
    ..Implications for future structural studies to address outstanding questions in the field are also discussed...
  67. pmc Identification of BRAF inhibitors through in silico screening
    Cheng Luo
    Wistar Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Med Chem 51:6121-7. 2008
    ..The binding modes of these inhibitors to BRAF are analyzed through molecular docking to derive structure-activity relationships and to assist in the future development of more potent and more specific BRAF inhibitors...
  68. pmc Crystal structure of the retinoblastoma tumor suppressor protein bound to E2F and the molecular basis of its regulation
    Bing Xiao
    Division of Protein Structure, National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
    Proc Natl Acad Sci U S A 100:2363-8. 2003
    ..Finally, our data reveal that the tight binding of the human papillomavirus E7 oncoprotein to pRb prevents subsequent interactions with the marked box region of E2F but not with its core binding region...
  69. pmc Human immunodeficiency virus type 1 Tat protein inhibits the SIRT1 deacetylase and induces T cell hyperactivation
    Hye Sook Kwon
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94158, USA
    Cell Host Microbe 3:158-67. 2008
    ..These events likely contribute to the state of immune cell hyperactivation found in HIV-infected individuals...
  70. doi request reprint Ankyrin for methylated lysines
    Michael M Brent
    Nat Struct Mol Biol 15:221-2. 2008
  71. pmc 14-3-3 interaction with histone H3 involves a dual modification pattern of phosphoacetylation
    Wendy Walter
    Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3 QU, United Kingdom
    Mol Cell Biol 28:2840-9. 2008
    ..The importance of acetylation in this interaction is also seen in vivo, where K14 acetylation is required for optimal Bmh1 recruitment to the GAL1 promoter during transcriptional activation...
  72. pmc Nuclear export modulates the cytoplasmic Sir2 homologue Hst2
    Jeanne M Wilson
    Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, 0347, La Jolla, California 92093, USA
    EMBO Rep 7:1247-51. 2006
    ..Our identification of putative nuclear export sequences in numerous vertebrate SIRT2 proteins shows that active nuclear export can be a conserved mechanism for regulating Sir2 homologues...
  73. pmc Multiple roles for acetylation in the interaction of p300 HAT with ATF-2
    Balasubramanyam Karanam
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Biochemistry 46:8207-16. 2007
    ..Taken together, these studies suggest multiple roles for protein acetylation in the regulation of transcription by p300/CBP and ATF-2...
  74. ncbi request reprint Acridine derivatives activate p53 and induce tumor cell death through Bax
    Wenge Wang
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, USA
    Cancer Biol Ther 4:893-8. 2005
    ..These findings provide insights into p53 regulation in response to DNA intercalating drugs and may assist new anti-cancer drug design...
  75. ncbi request reprint A mechanism-based inactivator for histone demethylase LSD1
    Jeffrey C Culhane
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Am Chem Soc 128:4536-7. 2006
    ..Compound 1 is thus a selective, mechanism-based inactivator of LSD1 and is likely to serve as a useful tool in the study of histone modifications and chromatin remodeling...

Research Grants4

  1. Predoctoral Training at the Chemistry-Biology Interface
    Ronen Marmorstein; Fiscal Year: 2007
    ....
  2. TRAINING PROGRAM IN BASIC CANCER RESEARCH
    Ronen Marmorstein; Fiscal Year: 2007
    ..Altogether, this training program offers training in "cutting edge" basic cancer research that will prepare the participating trainees for a productive career in science that will advance our understanding of the malignant process. ..