James D Marks

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies
    Michela Visintin
    International School for Advanced Studies SISSA and INFM Unit, 34013 Trieste, Italy
    J Mol Biol 317:73-83. 2002
  2. doi request reprint Impact of intrinsic affinity on functional binding and biological activity of EGFR antibodies
    Yu Zhou
    Department of Anesthesia, University of California, San Francisco, San Francisco General Hospital, San Francisco, California 94110, USA
    Mol Cancer Ther 11:1467-76. 2012
  3. pmc Extraction and inhibition of enzymatic activity of botulinum neurotoxins /B1, /B2, /B3, /B4, and /B5 by a panel of monoclonal anti-BoNT/B antibodies
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, N, E, Atlanta, GA 30341, USA
    BMC Biochem 12:58. 2011
  4. ncbi request reprint Selection of cell binding and internalizing epidermal growth factor receptor antibodies from a phage display library
    T Heitner
    Department of Anesthesia, University of California, San Francisco, Room 3C 38, San Francisco General Hospital, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    J Immunol Methods 248:17-30. 2001
  5. ncbi request reprint Deciphering antibody properties that lead to potent botulinum neurotoxin neutralization
    James D Marks
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco General Hospital, San Francisco, California 94110, USA
    Mov Disord 19:S101-8. 2004
  6. ncbi request reprint Medical aspects of biologic toxins
    James D Marks
    Department of Anesthesia and Perioperative Care, University of California San Francisco, San Francisco General Hospital, Room 3C38, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    Anesthesiol Clin North America 22:509-32, vii. 2004
  7. ncbi request reprint Anti-CD166 single chain antibody-mediated intracellular delivery of liposomal drugs to prostate cancer cells
    Audrey Roth
    Department of Anesthesia, University of California at San Francisco, Room 3C38, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    Mol Cancer Ther 6:2737-46. 2007
  8. pmc Human antibodies targeting cell surface antigens overexpressed by the hormone refractory metastatic prostate cancer cells: ICAM-1 is a tumor antigen that mediates prostate cancer cell invasion
    Fraser Conrad
    Department of Anesthesia, University of California at San Francisco, 1001 Potrero Ave, 3C38, San Francisco, CA 94110, USA
    J Mol Med (Berl) 87:507-14. 2009
  9. ncbi request reprint Therapeutic efficacy of anti-ErbB2 immunoliposomes targeted by a phage antibody selected for cellular endocytosis
    Ulrik B Nielsen
    Department of Anesthesia and Pharmaceutical Chemistry, University of California San Francisco, SF General Hospital, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    Biochim Biophys Acta 1591:109-118. 2002
  10. ncbi request reprint Phage versus phagemid libraries for generation of human monoclonal antibodies
    David O'Connell
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco, Rm 3C 38, San Francisco General Hospital, 1001 Potrero Ave, 94110, USA
    J Mol Biol 321:49-56. 2002

Collaborators

Detail Information

Publications57

  1. ncbi request reprint The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies
    Michela Visintin
    International School for Advanced Studies SISSA and INFM Unit, 34013 Trieste, Italy
    J Mol Biol 317:73-83. 2002
    ..The development of IACT, together with the possibility of scaling up in a high throughput and automated format, makes IACT a new enabling tool for target validation in functional genomics and global proteomics...
  2. doi request reprint Impact of intrinsic affinity on functional binding and biological activity of EGFR antibodies
    Yu Zhou
    Department of Anesthesia, University of California, San Francisco, San Francisco General Hospital, San Francisco, California 94110, USA
    Mol Cancer Ther 11:1467-76. 2012
    ..Overall, our work indicates that higher intrinsic affinity combined with bivalent binding can achieve avidity that leads to greater in vitro potency of antibodies, which may translate into greater therapeutic efficacy...
  3. pmc Extraction and inhibition of enzymatic activity of botulinum neurotoxins /B1, /B2, /B3, /B4, and /B5 by a panel of monoclonal anti-BoNT/B antibodies
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, N, E, Atlanta, GA 30341, USA
    BMC Biochem 12:58. 2011
    ..However, some antibodies inhibit or neutralize the enzymatic activity of BoNT, so the choice of antibody for toxin extraction is critical...
  4. ncbi request reprint Selection of cell binding and internalizing epidermal growth factor receptor antibodies from a phage display library
    T Heitner
    Department of Anesthesia, University of California, San Francisco, Room 3C 38, San Francisco General Hospital, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    J Immunol Methods 248:17-30. 2001
    ..Depending upon the format used, the antibodies can be used to deliver molecules to the cell surface or intracellularly...
  5. ncbi request reprint Deciphering antibody properties that lead to potent botulinum neurotoxin neutralization
    James D Marks
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco General Hospital, San Francisco, California 94110, USA
    Mov Disord 19:S101-8. 2004
    ..The very high affinity required for toxin neutralization suggests why single mAbs that potently neutralize toxin have not been reported. Such affinities are not typically generated by the immune response...
  6. ncbi request reprint Medical aspects of biologic toxins
    James D Marks
    Department of Anesthesia and Perioperative Care, University of California San Francisco, San Francisco General Hospital, Room 3C38, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    Anesthesiol Clin North America 22:509-32, vii. 2004
    ..The remainder of the article is devoted to sections on the other three biothreat toxins: ricin, staphylococcal enterotoxin B, and C perfringens epsilon toxin...
  7. ncbi request reprint Anti-CD166 single chain antibody-mediated intracellular delivery of liposomal drugs to prostate cancer cells
    Audrey Roth
    Department of Anesthesia, University of California at San Francisco, Room 3C38, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    Mol Cancer Ther 6:2737-46. 2007
    ..Further studies on intracellular tracking of endocytosed liposomal drugs will help identify and overcome the barriers limiting the potency of liposomal drugs...
  8. pmc Human antibodies targeting cell surface antigens overexpressed by the hormone refractory metastatic prostate cancer cells: ICAM-1 is a tumor antigen that mediates prostate cancer cell invasion
    Fraser Conrad
    Department of Anesthesia, University of California at San Francisco, 1001 Potrero Ave, 3C38, San Francisco, CA 94110, USA
    J Mol Med (Berl) 87:507-14. 2009
    ....
  9. ncbi request reprint Therapeutic efficacy of anti-ErbB2 immunoliposomes targeted by a phage antibody selected for cellular endocytosis
    Ulrik B Nielsen
    Department of Anesthesia and Pharmaceutical Chemistry, University of California San Francisco, SF General Hospital, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    Biochim Biophys Acta 1591:109-118. 2002
    ..This strategy should be applicable to generate immunotherapeutics for other malignancies by selecting phage antibodies for internalization into other tumor types and using the scFv to target liposomes or other nanoparticles...
  10. ncbi request reprint Phage versus phagemid libraries for generation of human monoclonal antibodies
    David O'Connell
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco, Rm 3C 38, San Francisco General Hospital, 1001 Potrero Ave, 94110, USA
    J Mol Biol 321:49-56. 2002
    ..This could be overcome by utilizing the scFv in a multivalent format, by affinity maturation, or by converting the library to monovalent display after the first round of selection...
  11. ncbi request reprint Impact of single-chain Fv antibody fragment affinity on nanoparticle targeting of epidermal growth factor receptor-expressing tumor cells
    Yu Zhou
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco Rm 3C 38, San Francisco General Hospital, 1001 Potrero Ave, San Francisco, CA 94110, USA
    J Mol Biol 371:934-47. 2007
    ..The results show the importance of antibody fragment density on nanoparticle uptake, and suggest that engineering ultrahigh affinity scFv may be unnecessary for optimal nanoparticle targeting...
  12. pmc A novel assay for monitoring internalization of nanocarrier coupled antibodies
    Ulrik B Nielsen
    Department of Anesthesia, University of California San Francisco, San Francisco, CA 94110, USA
    BMC Immunol 7:24. 2006
    ..We have termed this methodology "Chelated Ligand Internalization Assay", or CLIA...
  13. doi request reprint Discovery of internalizing antibodies to tumor antigens from phage libraries
    Yu Zhou
    Department of Anesthesia and Perioperative Care, University of California, San Francisco, California, USA
    Methods Enzymol 502:43-66. 2012
    ....
  14. ncbi request reprint Recombinant full-length human IgG1s targeting hormone-refractory prostate cancer
    Bin Liu
    Department of Anesthesia, University of California at San Francisco, 1001 Potrero Avenue, 3C 38, San Francisco, CA 94110, USA
    J Mol Med (Berl) 85:1113-23. 2007
    ....
  15. ncbi request reprint Epidermal growth factor receptor (EGFR)-targeted immunoliposomes mediate specific and efficient drug delivery to EGFR- and EGFRvIII-overexpressing tumor cells
    Christoph Mamot
    Division of Hematology Oncology, University of California, San Francisco, 94115, USA
    Cancer Res 63:3154-61. 2003
    ..We conclude that EGFR-targeted ILs provide efficient and targeted delivery of anticancer drugs in cells overexpressing EGFR or EGFRvIII...
  16. pmc Diabodies targeting epithelial membrane protein 2 reduce tumorigenicity of human endometrial cancer cell lines
    Kaori Shimazaki
    Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Clin Cancer Res 14:7367-77. 2008
    ..The present study assesses the suitability of EMP2 as a therapeutic target...
  17. doi request reprint Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast-displayed tumor antigens
    Yu Zhou
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco, Room 3C 38, San Francisco General Hospital, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    J Mol Biol 404:88-99. 2010
    ..This approach is generalizable to many mammalian cell surface proteins, results in the generation of functional internalizing antibodies, and does not require antigen expression and purification for antibody generation...
  18. doi request reprint Identification of target and function specific antibodies for effective drug delivery
    Yu Zhou
    University of California, San Francisco, CA, USA
    Methods Mol Biol 525:145-60, xv. 2009
    ....
  19. doi request reprint Selection and characterization of cell binding and internalizing phage antibodies
    Yu Zhou
    Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USA
    Arch Biochem Biophys 526:107-13. 2012
    ..These factors include the cell types used for selection, the impact of different phage antibody library formats, and the specific selection protocols used...
  20. ncbi request reprint Using phage display to select antibodies recognizing post-translational modifications independently of sequence context
    John W Kehoe
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, and Department of Anesthesia and Pharmaceutical Chemistry, San Francisco General Hospital 94110, USA
    Mol Cell Proteomics 5:2350-63. 2006
    ....
  21. ncbi request reprint Mapping tumor epitope space by direct selection of single-chain Fv antibody libraries on prostate cancer cells
    Bin Liu
    Department of Anesthesia and Pharmaceutical Chemistry, University of California at San Francisco, San Francisco General Hospital, California, USA
    Cancer Res 64:704-10. 2004
    ..Targeting components of this epitope space may facilitate development of immunotherapeutic and small molecule-based strategies as well as the use of other therapeutic agents that rely upon delivery to the interior of the tumor cell...
  22. pmc Tumor detection by imaging proteolytic activity
    Molly R Darragh
    Graduate Group in Biophysics, Department of Pharmaceutical Chemistry, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, California 94158, USA
    Cancer Res 70:1505-12. 2010
    ..Our findings define MT-SP1 activity as a useful biomarker to visualize epithelial cancers using a noninvasive antibody-based method...
  23. pmc A single-domain llama antibody potently inhibits the enzymatic activity of botulinum neurotoxin by binding to the non-catalytic alpha-exosite binding region
    Jianbo Dong
    Department of Anesthesia, University of California, San Francisco, San Francisco, CA 94110, USA
    J Mol Biol 397:1106-18. 2010
    ..The study validates the utility of non-immune llama VHH libraries as a source of enzyme inhibitors and identifies the BoNT/A Lc alpha-exosite as a target for inhibitor development...
  24. ncbi request reprint Genetic and immunological comparison of anti-botulinum type A antibodies from immune and non-immune human phage libraries
    Peter Amersdorfer
    Department of Anesthesia and Pharmaceutical Chemistry, San Francisco General Hospital, University of California, San Francisco Rm 3C 38, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    Vaccine 20:1640-8. 2002
    ..We therefore suggest that a vaccine based on the pentavalent botulinum toxoid directs the humoral immune response to a limited number of immunodominant epitopes exposed on the binding domain HC...
  25. ncbi request reprint Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin
    Consuelo Garcia-Rodriguez
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco Rm 3C 38, San Francisco General Hospital, 1001 Potrero Ave, San Francisco, California 94110, USA
    Nat Biotechnol 25:107-16. 2007
    ..The results show how an antibody can be engineered to bind two different antigens despite structural differences in the antigen-antibody interface and may provide a general strategy for tuning antibody specificity and cross-reactivity...
  26. ncbi request reprint Anti-HER2 immunoliposomes: enhanced efficacy attributable to targeted delivery
    John W Park
    Division of Hematology Oncology, University of California, San Francisco, San Francisco, California 94143, USA
    Clin Cancer Res 8:1172-81. 2002
    ..We previously showed that anti-HER2 immunoliposomes bind efficiently to and internalize in HER2-overexpressing cells in vitro, resulting in intracellular drug delivery...
  27. ncbi request reprint Selection of human antibodies from phage display libraries
    James D Marks
    Department of Anesthesia, University of California, San Francisco, San Francisco General Hospital, USA
    Methods Mol Biol 248:161-76. 2004
  28. ncbi request reprint Selection of internalizing antibodies for drug delivery
    James D Marks
    Department of Anesthesia, University of California, San Francisco, San Francisco General Hospital, USA
    Methods Mol Biol 248:201-8. 2004
  29. ncbi request reprint Phage-display antibody detection of Chlamydia trachomatis-associated antigens
    Erika A Lindquist
    Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720 7360, USA
    Microbiology 148:443-51. 2002
    ....
  30. ncbi request reprint Antibody affinity maturation by chain shuffling
    James D Marks
    Department of Anesthesia, University of California, San Francisco, San Francisco General Hospital, USA
    Methods Mol Biol 248:327-43. 2004
  31. pmc N-Terminal labeling of filamentous phage to create cancer marker imaging agents
    Zachary M Carrico
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    ACS Nano 6:6675-80. 2012
    ....
  32. ncbi request reprint Towards proteome-wide production of monoclonal antibody by phage display
    Bin Liu
    Department of Anesthesia, University of California, San Francisco, Rm 3C 38, San Francisco General Hospital, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    J Mol Biol 315:1063-73. 2002
    ..Automation of this process should allow high throughput production of monoclonal phage antibodies against cellular proteins as well as proteins that are uniquely expressed under pathological conditions...
  33. ncbi request reprint PCR cloning of human immunoglobulin genes
    James D Marks
    Department of Anesthesia, University of California, San Francisco, San Francisco General Hospital, USA
    Methods Mol Biol 248:117-34. 2004
  34. ncbi request reprint Avidity-mediated enhancement of in vivo tumor targeting by single-chain Fv dimers
    Gregory P Adams
    Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Clin Cancer Res 12:1599-605. 2006
    ..25 %ID/g). These findings substantiate that the improved tumor retention of (sFv')2 homodimers over sFv monomers results from the availability of dual binding sites rather than from the slower systemic clearance of homodimers...
  35. pmc Effective treatment of established human breast tumor xenografts in immunodeficient mice with a single dose of the alpha-emitting radioisotope astatine-211 conjugated to anti-HER2/neu diabodies
    Matthew K Robinson
    Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Clin Cancer Res 14:875-82. 2008
    ..We hypothesized that the C6.5 diabody, a noncovalent anti-HER2 single-chain Fv dimer, would be an ideal radioisotope carrier for the radioimmunotherapy of established tumors using the short-lived alpha-emitting radioisotope (211)At...
  36. doi request reprint Development of human single-chain antibodies against SARS-associated coronavirus
    K M Leung
    CK Life Sciences International Inc, Hong Kong, SAR, China
    Intervirology 51:173-81. 2008
    ..We screened a phage displaying library of human single-chain antibodies (ScFv) against the predicted epitopes and obtained a human ScFv which can recognize the SARS virus in vitro...
  37. ncbi request reprint Predicting antigenic peptides suitable for the selection of phage antibodies
    Peter Pavlik
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Hum Antibodies 12:99-112. 2003
    ..This is likely to have considerable utility in functional genomics and proteomics where it should be possible to select antibodies against gene products on the basis of deduced amino acid sequence in a high throughput fashion...
  38. ncbi request reprint Isolation of scFvs to in vitro produced extracellular domains of EGFR family members
    Eva Horak
    Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Cancer Biother Radiopharm 20:603-13. 2005
    ..These scFvs provide a valuable and potentially clinically relevant panel of agents to target the members of the EGFR family...
  39. ncbi request reprint Characterization of a single-chain intrabody directed against the human receptor tyrosine kinase Ron
    Paola Secco
    Department of Medical Sciences and Interdisciplinary Research Center of Autoimmune Diseases IRCAD, University of Eastern Piedmont A Avogadro, Via Solaroli 17, 28100 Novara, Italy
    J Immunol Methods 285:99-109. 2004
    ..We demonstrate that the isolated antibody fragment interacts in vivo with the intracellular domain of Ron in mammalian cells...
  40. ncbi request reprint The cleavage site of C5 from man and animals as a common target for neutralizing human monoclonal antibodies: in vitro and in vivo studies
    Roberto Marzari
    Department of Biology, University of Trieste, Via Fleming 22, I 34127 Trieste, Italy
    Eur J Immunol 32:2773-82. 2002
    ..The scFv TS-A12/22 was tested in a rat model of antigen-induced arthritis and proved to be effective in preventing influx of polymorphonuclear cells into the knee joint and C9 deposition on synovial tissue...
  41. ncbi request reprint Generation and characterization of a protective monoclonal antibody to Pseudomonas aeruginosa PcrV
    Dara W Frank
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    J Infect Dis 186:64-73. 2002
    ..aeruginosa when coinstilled with the bacterial inoculum or intraperitoneally transferred to mice. Fab fragments from MAb 166 prevent sepsis and death. The epitope bound by MAb 166 was mapped to the carboxyl-terminus of PcrV...
  42. pmc Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids
    Theresa J Smith
    Integrated Toxicology Division, United States Army Medical Institute of Infectious Diseases, Fort Detrick, Maryland, United States of America
    PLoS ONE 2:e1271. 2007
    ..The BoNTs are located within two generally conserved gene arrangements known as botulinum progenitor complexes which encode toxin-associated proteins involved in toxin stability and expression...
  43. pmc Identification and characterization of tumor antigens by using antibody phage display and intrabody strategies
    Anne Laure Goenaga
    ENS Cachan Laboratoire de Biotechnologie et Pharmacologie Génétique Appliquée LBPA, UMR CNRS 8113, 61 Avenue du President Wilson, 94235 Cachan Cedex, France
    Mol Immunol 44:3777-88. 2007
    ..This study is the proof of principle that the direct selection of phage antibody libraries on tumor cells can effectively lead to the identification and functional characterization of relevant tumor markers...
  44. ncbi request reprint Development of ligand-targeted liposomes for cancer therapy
    Charles O Noble
    University of California at San Francisco, CA 94143, USA
    Expert Opin Ther Targets 8:335-53. 2004
    ..Anti-HER2 immunoliposomal doxorubicin is awaiting Phase I clinical trials, the results of which should provide new insights into the promise of ligand-targeted liposomal therapies...
  45. ncbi request reprint Antibody targeting of long-circulating lipidic nanoparticles does not increase tumor localization but does increase internalization in animal models
    Dmitri B Kirpotin
    Hermes Biosciences Inc, South San Francisco, CA, USA
    Cancer Res 66:6732-40. 2006
    ..Immunoliposomes capable of selective internalization in cancer cells in vivo may provide new opportunities for drug delivery...
  46. ncbi request reprint Preclinical manufacture of anti-HER2 liposome-inserting, scFv-PEG-lipid conjugate. 2. Conjugate micelle identity, purity, stability, and potency analysis
    David F Nellis
    SAIC Frederick, Inc, National Cancer Institute at Frederick, PO Box B, Frederick, Maryland 21702, USA
    Biotechnol Prog 21:221-32. 2005
    ....
  47. ncbi request reprint Preclinical manufacture of an anti-HER2 scFv-PEG-DSPE, liposome-inserting conjugate. 1. Gram-scale production and purification
    David F Nellis
    SAIC Frederick, Inc, National Cancer Institute at Frederick, PO Box B, Frederick, Maryland 21702, USA
    Biotechnol Prog 21:205-20. 2005
    ..Residual endotoxin, rProtein A, and genomic DNA, were at acceptable levels. This study successfully addressed a necessary step in the scale-up of immunoliposome-encapsulated therapeutics...
  48. ncbi request reprint Quantitative immuno-positron emission tomography imaging of HER2-positive tumor xenografts with an iodine-124 labeled anti-HER2 diabody
    Matthew K Robinson
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
    Cancer Res 65:1471-8. 2005
    ..Thus, diabodies may represent an effective molecular structure for development of novel PET radiotracers...
  49. ncbi request reprint A single treatment of yttrium-90-labeled CHX-A"-C6.5 diabody inhibits the growth of established human tumor xenografts in immunodeficient mice
    Gregory P Adams
    Divison of Medical Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Cancer Res 64:6200-6. 2004
    ..The maximum tolerated dose was also dependent on the tumor xenograft model used. These studies indicate that genetically engineered antitumor diabody molecules can be used as effective vehicles for radioimmunotherapy...
  50. ncbi request reprint Regulation of antibody-dependent cellular cytotoxicity by IgG intrinsic and apparent affinity for target antigen
    Yong Tang
    Department of Medical Oncology, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    J Immunol 179:2815-23. 2007
    ..Because high affinity may impair in vivo tumor targeting, a careful examination of Ab structure to function relationships is required to develop optimized therapeutic unconjugated Abs...
  51. pmc Affinity thresholds for membrane fusion triggering by viral glycoproteins
    Kosei Hasegawa
    Molecular Medicine Program, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    J Virol 81:13149-57. 2007
    ..For a given cell surface receptor density, there is an affinity threshold above which cell-cell fusion proceeds efficiently. Suprathreshold affinities do not further enhance the efficiency of membrane fusion...
  52. ncbi request reprint Redirected activity of human antitumor chimeric immune receptors is governed by antigen and receptor expression levels and affinity of interaction
    Fabio Turatti
    Molecular Therapies Unit, Department of Experimental Oncology, Istituto Nazionale Tumori, Milan, Italy
    J Immunother 30:684-93. 2007
    ..On the contrary, low antigen-expressing tumor variants could be eliminated by decreasing CIR affinity. Tuning CIR expression and affinity might help in discriminating different biologic contexts...
  53. ncbi request reprint Yeast mating for combinatorial Fab library generation and surface display
    Jane M Weaver-Feldhaus
    Pacific Northwest National Laboratory, MSIN K4 12, 902 Battelle Boulevard, P O Box 999, Richland, WA 99352, USA
    FEBS Lett 564:24-34. 2004
    ..Through yeast mating of the haploid libraries, a very large heterodimeric immune Fab library was displayed on the diploids and high affinity antigen specific Fabs were isolated from the library...
  54. ncbi request reprint Antibodies from phage antibody libraries
    Andrew R M Bradbury
    Bioscience Division, Los Alamos National Laboratory, TA 43, HRL 1, MS M888, NM 87545, USA
    J Immunol Methods 290:29-49. 2004
  55. ncbi request reprint Novel application of an in vitro technique to the detection and quantification of botulinum neurotoxin antibodies
    Yper H J Hall
    Centre for Applied Microbiology and Research, Health Protection Agency Porton Down, Porton Down, Salisbury, Wiltshire SP4 0JG, UK
    J Immunol Methods 288:55-60. 2004
    ..The results support the conclusion that the detection of neutralising antibodies in human sera should be attempted using this method...
  56. ncbi request reprint A structural perspective of the sequence variability within botulinum neurotoxin subtypes A1-A4
    Joseph W Arndt
    Department of Molecular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 362:733-42. 2006
    ..In particular, sequence variation in subtypes BoNT/A3 and BoNT/A4 will likely effect alpha-exosite and S1' subsite recognition, respectively...
  57. ncbi request reprint Molecular architecture of botulinum neurotoxin E revealed by single particle electron microscopy
    Audrey Fischer
    Section of Neurobiology, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093 0366, USA
    J Biol Chem 283:3997-4003. 2008
    ..We postulate that the unique architecture of functionally conserved modules underlies the distinguishing attributes of BoNT/E and contributes to differences with BoNT/A...

Research Grants4

  1. Deciphering toxin neutralization by oligoclonal antibody
    James Marks; Fiscal Year: 2003
    ..In addition, this approach would be applicable to four of the other Class A agents (anthrax, smallpox, plague, and hemorrhagic fever viruses). ..
  2. Development of botulinum neurotoxin immunotherapy
    James Marks; Fiscal Year: 2007
    ..Such information will be invaluable for vaccine development as well as diagnostic testing and microbial forensics. ..
  3. Development of botulinum neurotoxin immunotherapy, serotypes C, D, F, and G
    James Marks; Fiscal Year: 2007
    ..Combined with previous results, this will make available a safe, efficacious human compatible antitoxin for all seven BoNT serotypes. ..