Martin Marinus

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. pmc DNA methylation and mutator genes in Escherichia coli K-12
    Martin G Marinus
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Mutat Res 705:71-6. 2010
  2. pmc Recombination is essential for viability of an Escherichia coli dam (DNA adenine methyltransferase) mutant
    M G Marinus
    Department of Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 182:463-8. 2000
  3. pmc Separation of mutation avoidance and antirecombination functions in an Escherichia coli mutS mutant
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School 364 Plantation Street, Worcester, MA 01605, USA
    Nucleic Acids Res 33:1193-200. 2005
  4. pmc MutS inhibits RecA-mediated strand exchange with platinated DNA substrates
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 101:14174-9. 2004
  5. pmc MutS inhibits RecA-mediated strand transfer with methylated DNA substrates
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School Worcester, MA 01605, USA
    Nucleic Acids Res 33:3591-7. 2005
  6. ncbi request reprint Cisplatin induces DNA double-strand break formation in Escherichia coli dam mutants
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    DNA Repair (Amst) 4:773-81. 2005
  7. pmc Dominant negative mutator mutations in the mutS gene of Escherichia coli
    T H Wu
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    J Bacteriol 176:5393-400. 1994
  8. pmc The MutS C terminus is essential for mismatch repair activity in vivo
    Melissa A Calmann
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    J Bacteriol 187:6577-9. 2005
  9. pmc Repair of heteroduplex DNA molecules with multibase loops in Escherichia coli
    M Carraway
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    J Bacteriol 175:3972-80. 1993
  10. pmc The function of Asp70, Glu77 and Lys79 in the Escherichia coli MutH protein
    Te hui Wu
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA
    Nucleic Acids Res 30:818-22. 2002

Research Grants

  1. DNA Mismatch and Double-Strand Break Repair
    Martin Marinus; Fiscal Year: 2005
  2. DNA Mismatch and Double-Strand Break Repair
    Martin Marinus; Fiscal Year: 2009
  3. DNA Mismatch and Double-Strand Break Repair
    Martin G Marinus; Fiscal Year: 2010

Collaborators

Detail Information

Publications31

  1. pmc DNA methylation and mutator genes in Escherichia coli K-12
    Martin G Marinus
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Mutat Res 705:71-6. 2010
    ..This review gives a personal perspective on the discovery of dam mutants in E. coli and their relationship to mismatch repair and mutator phenotypes...
  2. pmc Recombination is essential for viability of an Escherichia coli dam (DNA adenine methyltransferase) mutant
    M G Marinus
    Department of Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 182:463-8. 2000
    ..The requirement for recombination also suggests an explanation for the sensitivity of dam cells to certain DNA-damaging agents...
  3. pmc Separation of mutation avoidance and antirecombination functions in an Escherichia coli mutS mutant
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School 364 Plantation Street, Worcester, MA 01605, USA
    Nucleic Acids Res 33:1193-200. 2005
    ..The inability of MutSDelta800 to form tetramers may indicate that these are the active form of MutS...
  4. pmc MutS inhibits RecA-mediated strand exchange with platinated DNA substrates
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 101:14174-9. 2004
    ..MutL addition was without effect even in the presence of MutS. The results suggest that although mismatch repair is beneficial for mutation avoidance, its antirecombination activity on inappropriate substrates can be lethal to the cell...
  5. pmc MutS inhibits RecA-mediated strand transfer with methylated DNA substrates
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School Worcester, MA 01605, USA
    Nucleic Acids Res 33:3591-7. 2005
    ..These results are consistent with a model in which methylated DNA is perceived by the cell as homeologous and prevented from recombining with homologous DNA by the MMR system...
  6. ncbi request reprint Cisplatin induces DNA double-strand break formation in Escherichia coli dam mutants
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    DNA Repair (Amst) 4:773-81. 2005
    ..We show that dam priA strains are not viable suggesting that DSB formation is dependent on DNA replication restart. The sensitivity of priA mutants to cisplatin is also consistent with this conclusion...
  7. pmc Dominant negative mutator mutations in the mutS gene of Escherichia coli
    T H Wu
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    J Bacteriol 176:5393-400. 1994
    ..There was a direct correlation between the levels of recombination and mutagenesis in the mutant strains, suggesting that these phenotypes are due to the same function of MutS...
  8. pmc The MutS C terminus is essential for mismatch repair activity in vivo
    Melissa A Calmann
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    J Bacteriol 187:6577-9. 2005
    ..This strain has a MutS null phenotype for mutation avoidance, anti-recombination, and sensitivity to cytotoxic agents in a dam mutant background...
  9. pmc Repair of heteroduplex DNA molecules with multibase loops in Escherichia coli
    M Carraway
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    J Bacteriol 175:3972-80. 1993
    ..A high efficiency of repair (95%) was found even when the mismatch and the loops were 1,448 nucleotides apart. We conclude that multibase loops in DNA can be removed only as a consequence of corepair by dam-directed mismatch repair...
  10. pmc The function of Asp70, Glu77 and Lys79 in the Escherichia coli MutH protein
    Te hui Wu
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA
    Nucleic Acids Res 30:818-22. 2002
    ..Although the data are consistent with the prediction of a catalytic role for Asp70, Glu77 and Lys79, it cannot be excluded that they are also involved in binding to MutL...
  11. ncbi request reprint Deletion mutation analysis of the mutS gene in Escherichia coli
    T H Wu
    Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 274:5948-52. 1999
    ..Given the extensive amino acid homology in the MutS family our results with E. coli should be applicable to MutS homologues in other prokaryotes and eukaryotes...
  12. pmc Homologous recombination prevents methylation-induced toxicity in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Nucleic Acids Res 34:2258-68. 2006
    ....
  13. ncbi request reprint Spontaneous and cisplatin-induced recombination in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, LRB823 Worcester, MA 01655, USA
    DNA Repair (Amst) 3:719-28. 2004
    ..The lack of recombination induction by trans-DDP suggests that the recombinogenic lesions for cisplatin are purine-purine intrastrand crosslinks...
  14. pmc Regulated expression of the Escherichia coli dam gene
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 185:5012-4. 2003
    ..Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease...
  15. ncbi request reprint Mutational analysis of the MutH protein from Escherichia coli
    T Loh
    Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 276:12113-9. 2001
    ..Two deletion mutations (MutHDelta224 and MutHDelta214) in the C-terminal end of the protein, localized the MutL stimulation region to five amino acids (Ala-220, Leu-221, Leu-222, Ala-223, and Arg-224)...
  16. ncbi request reprint Growth-rate-dependent transcription initiation from the dam P2 promoter
    L J Rasmussen
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655, USA
    Gene 157:213-5. 1995
    ..The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point...
  17. ncbi request reprint Novel growth rate control of dam gene expression in Escherichia coli
    L J Rasmussen
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    Mol Microbiol 12:631-8. 1994
    ..The cde-4::miniTn10 insertion is located close to kilobase 670 on the physical map in or near the lipB gene...
  18. ncbi request reprint Identification of a weak promoter for the dam gene of Escherichia coli
    T H Wu
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    Biochim Biophys Acta 1131:47-52. 1992
    ..No ribosome binding sequence was present close to the ATG codon suggesting that the transcript may be inefficiently translated...
  19. pmc DNA mismatch repair-induced double-strand breaks
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    DNA Repair (Amst) 7:48-56. 2008
    ..This model is consistent with the observation that fast-growing dam recB(Ts) ada ogt cells, which have more chromosome replication origins, are more sensitive to the cytotoxic effect of MNNG than the same cells growing slowly...
  20. ncbi request reprint Specificity of Escherichia coli mutD and mutL mutator strains
    T H Wu
    Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
    Gene 87:1-5. 1990
    ..These results suggest that the proofreading function of DNA polymerase III primarily repairs potential transversion mutations while Dam-dependent mismatch repair rectifies potential transition mutations...
  21. pmc Dominant negative mutator mutations in the mutL gene of Escherichia coli
    A Aronshtam
    Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucleic Acids Res 24:2498-504. 1996
    ..All but one of the sequence changes affecting the C-terminal end of the protein are nonsense mutations...
  22. ncbi request reprint Increased adherence and actin pedestal formation by dam-deficient enterohaemorrhagic Escherichia coli O157:H7
    Kenneth G Campellone
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Microbiol 63:1468-81. 2007
    ..In contrast to other dam-deficient pathogens, EHECDeltadam is capable of robust intestinal colonization of experimentally infected animals...
  23. pmc Differential effects of cisplatin and MNNG on dna mutants of Escherichia coli
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mutat Res 578:406-16. 2005
    ..The mutation spectrum in the dnaN159 strain was consistent with increased SOS induction and not indicative of MMR deficiency...
  24. ncbi request reprint Dam methylation: coordinating cellular processes
    Anders Løbner-Olesen
    Department of Life Sciences and Chemistry, Roskilde University, DK 4000 Roskilde, Denmark
    Curr Opin Microbiol 8:154-60. 2005
    ..DamMT has also been implicated as a virulence factor in bacterial pathogenesis. The origin and phylogeny of DamMT, based on sequenced genomes, has been deduced...
  25. ncbi request reprint The beta sliding clamp binds to multiple sites within MutL and MutS
    Francisco J López de Saro
    The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 281:14340-9. 2006
    ..In light of these results, we propose roles for the beta clamp in orchestrating the sequence of events that lead to mismatch repair in the cell...
  26. ncbi request reprint RecN and RecG are required for Escherichia coli survival of Bleomycin-induced damage
    Jessica L Kosa
    Biological Engineering Division, Department of Chemistry, Massachusetts Institute of Technology, Rm 56 689, 77 Massachusetts Avenue, Cambridge 02139, USA
    Mutat Res 554:149-57. 2004
    ..The most straightforward explanation of these results is that DSB repair involves a structure that serves as a good substrate for RecG, and not RuvAB...
  27. pmc Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome
    Leise Riber
    Department of Life Sciences and Chemistry, Roskilde University, Denmark
    Genes Dev 20:2121-34. 2006
    ..Based on these observations, we propose that both RIDA and dnaA gene autoregulation are required as homeostatic mechanisms to ensure that initiation of replication occurs at the same time relative to cell mass in each cell cycle...
  28. ncbi request reprint MutS preferentially recognizes cisplatin- over oxaliplatin-modified DNA
    Zoran Z Zdraveski
    Department of Chemistry and Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 277:1255-60. 2002
    ..The differential affinity of MutS for DNA modified with the different platinum analogs could provide the molecular basis for the distinctive cellular responses to cisplatin and oxaliplatin...
  29. pmc YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica
    Sarah E Broadbent
    Department of Biology, IIU, Area 12, University of York, P O Box 373, York Y010 5YW, England
    J Bacteriol 189:4325-7. 2007
    ..This study provides evidence that the E. coli yhdJ gene encodes a DNA adenine methyltransferase. In contrast to an earlier report, however, we show that yhdJ is not an essential gene in either E. coli or S. enterica...
  30. pmc Nitric oxide-induced homologous recombination in Escherichia coli is promoted by DNA glycosylases
    Erik J Spek
    Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 184:3501-7. 2002
    ..These studies shed light on the underlying mechanism of NO*-induced homologous recombination...
  31. pmc Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis
    Anders Løbner-Olesen
    Department of Life Sciences and Chemistry, Roskilde University, DK 4000 Roskilde, Denmark
    Proc Natl Acad Sci U S A 100:4672-7. 2003
    ..We suggest that the methylation status of the cell is an important factor in forming and/or maintaining chromosome structure...

Research Grants9

  1. DNA Mismatch and Double-Strand Break Repair
    Martin Marinus; Fiscal Year: 2005
    ..If these experiments support our model, it will open up a new approach to combat tumor cells by targeting recombination proteins. ..
  2. DNA Mismatch and Double-Strand Break Repair
    Martin Marinus; Fiscal Year: 2009
    ..It also impacts the mechanism by which pathogenic bacteria become resistant to antibiotics and how this resistance is disseminated. ..
  3. DNA Mismatch and Double-Strand Break Repair
    Martin G Marinus; Fiscal Year: 2010
    ..It also impacts the mechanism by which pathogenic bacteria become resistant to antibiotics and how this resistance is disseminated. ..