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Genomes and Genes | Martin MarinusSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
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Publications
DNA methylation and mutator genes in Escherichia coli K-12Martin G Marinus
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Mutat Res 705:71-6. 2010..This review gives a personal perspective on the discovery of dam mutants in E. coli and their relationship to mismatch repair and mutator phenotypes...- Recombination is essential for viability of an Escherichia coli dam (DNA adenine methyltransferase) mutantM G Marinus
Department of Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Bacteriol 182:463-8. 2000..The requirement for recombination also suggests an explanation for the sensitivity of dam cells to certain DNA-damaging agents... - Separation of mutation avoidance and antirecombination functions in an Escherichia coli mutS mutantMelissa A Calmann
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School 364 Plantation Street, Worcester, MA 01605, USA
Nucleic Acids Res 33:1193-200. 2005..The inability of MutSDelta800 to form tetramers may indicate that these are the active form of MutS... - MutS inhibits RecA-mediated strand exchange with platinated DNA substratesMelissa A Calmann
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 101:14174-9. 2004..MutL addition was without effect even in the presence of MutS. The results suggest that although mismatch repair is beneficial for mutation avoidance, its antirecombination activity on inappropriate substrates can be lethal to the cell... - MutS inhibits RecA-mediated strand transfer with methylated DNA substratesMelissa A Calmann
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School Worcester, MA 01605, USA
Nucleic Acids Res 33:3591-7. 2005..These results are consistent with a model in which methylated DNA is perceived by the cell as homeologous and prevented from recombining with homologous DNA by the MMR system... 
Cisplatin induces DNA double-strand break formation in Escherichia coli dam mutantsAnetta Nowosielska
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
DNA Repair (Amst) 4:773-81. 2005..We show that dam priA strains are not viable suggesting that DSB formation is dependent on DNA replication restart. The sensitivity of priA mutants to cisplatin is also consistent with this conclusion...- Dominant negative mutator mutations in the mutS gene of Escherichia coliT H Wu
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
J Bacteriol 176:5393-400. 1994..There was a direct correlation between the levels of recombination and mutagenesis in the mutant strains, suggesting that these phenotypes are due to the same function of MutS... - The MutS C terminus is essential for mismatch repair activity in vivoMelissa A Calmann
Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
J Bacteriol 187:6577-9. 2005..This strain has a MutS null phenotype for mutation avoidance, anti-recombination, and sensitivity to cytotoxic agents in a dam mutant background... - Repair of heteroduplex DNA molecules with multibase loops in Escherichia coliM Carraway
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
J Bacteriol 175:3972-80. 1993..A high efficiency of repair (95%) was found even when the mismatch and the loops were 1,448 nucleotides apart. We conclude that multibase loops in DNA can be removed only as a consequence of corepair by dam-directed mismatch repair... - The function of Asp70, Glu77 and Lys79 in the Escherichia coli MutH proteinTe-hui Wu
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA
Nucleic Acids Res 30:818-22. 2002..Although the data are consistent with the prediction of a catalytic role for Asp70, Glu77 and Lys79, it cannot be excluded that they are also involved in binding to MutL... - Deletion mutation analysis of the mutS gene in Escherichia coliT H Wu
Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 274:5948-52. 1999..Given the extensive amino acid homology in the MutS family our results with E. coli should be applicable to MutS homologues in other prokaryotes and eukaryotes... - Homologous recombination prevents methylation-induced toxicity in Escherichia coliAnetta Nowosielska
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Nucleic Acids Res 34:2258-68. 2006.... - Spontaneous and cisplatin-induced recombination in Escherichia coliAnetta Nowosielska
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, LRB823 Worcester, MA 01655, USA
DNA Repair (Amst) 3:719-28. 2004..The lack of recombination induction by trans-DDP suggests that the recombinogenic lesions for cisplatin are purine-purine intrastrand crosslinks... - Regulated expression of the Escherichia coli dam geneMelissa A Calmann
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Bacteriol 185:5012-4. 2003..Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease... - Mutational analysis of the MutH protein from Escherichia coliT Loh
Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 276:12113-9. 2001..Two deletion mutations (MutHDelta224 and MutHDelta214) in the C-terminal end of the protein, localized the MutL stimulation region to five amino acids (Ala-220, Leu-221, Leu-222, Ala-223, and Arg-224)... - Growth-rate-dependent transcription initiation from the dam P2 promoterL J Rasmussen
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655, USA
Gene 157:213-5. 1995..The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point... - Novel growth rate control of dam gene expression in Escherichia coliL J Rasmussen
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
Mol Microbiol 12:631-8. 1994..The cde-4::miniTn10 insertion is located close to kilobase 670 on the physical map in or near the lipB gene... - Identification of a weak promoter for the dam gene of Escherichia coliT H Wu
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
Biochim Biophys Acta 1131:47-52. 1992..No ribosome binding sequence was present close to the ATG codon suggesting that the transcript may be inefficiently translated... - DNA mismatch repair-induced double-strand breaksAnetta Nowosielska
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
DNA Repair (Amst) 7:48-56. 2008..This model is consistent with the observation that fast-growing dam recB(Ts) ada ogt cells, which have more chromosome replication origins, are more sensitive to the cytotoxic effect of MNNG than the same cells growing slowly... - Specificity of Escherichia coli mutD and mutL mutator strainsT H Wu
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655
Gene 87:1-5. 1990..These results suggest that the proofreading function of DNA polymerase III primarily repairs potential transversion mutations while Dam-dependent mismatch repair rectifies potential transition mutations... - Dominant negative mutator mutations in the mutL gene of Escherichia coliA Aronshtam
Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Nucleic Acids Res 24:2498-504. 1996..All but one of the sequence changes affecting the C-terminal end of the protein are nonsense mutations... - Increased adherence and actin pedestal formation by dam-deficient enterohaemorrhagic Escherichia coli O157:H7Kenneth G Campellone
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Mol Microbiol 63:1468-81. 2007..In contrast to other dam-deficient pathogens, EHECDeltadam is capable of robust intestinal colonization of experimentally infected animals... - Differential effects of cisplatin and MNNG on dna mutants of Escherichia coliMelissa A Calmann
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Mutat Res 578:406-16. 2005..The mutation spectrum in the dnaN159 strain was consistent with increased SOS induction and not indicative of MMR deficiency... - Dam methylation: coordinating cellular processesAnders Løbner-Olesen
Department of Life Sciences and Chemistry, Roskilde University, DK 4000 Roskilde, Denmark
Curr Opin Microbiol 8:154-60. 2005..DamMT has also been implicated as a virulence factor in bacterial pathogenesis. The origin and phylogeny of DamMT, based on sequenced genomes, has been deduced... - The beta sliding clamp binds to multiple sites within MutL and MutSFrancisco J López de Saro
The Rockefeller University, New York, New York 10021, USA
J Biol Chem 281:14340-9. 2006..In light of these results, we propose roles for the beta clamp in orchestrating the sequence of events that lead to mismatch repair in the cell... - RecN and RecG are required for Escherichia coli survival of Bleomycin-induced damageJessica L Kosa
Biological Engineering Division, Department of Chemistry, Massachusetts Institute of Technology, Rm 56 689, 77 Massachusetts Avenue, Cambridge 02139, USA
Mutat Res 554:149-57. 2004..The most straightforward explanation of these results is that DSB repair involves a structure that serves as a good substrate for RecG, and not RuvAB... - Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosomeLeise Riber
Department of Life Sciences and Chemistry, Roskilde University, Denmark
Genes Dev 20:2121-34. 2006..Based on these observations, we propose that both RIDA and dnaA gene autoregulation are required as homeostatic mechanisms to ensure that initiation of replication occurs at the same time relative to cell mass in each cell cycle... - MutS preferentially recognizes cisplatin- over oxaliplatin-modified DNAZoran Z Zdraveski
Department of Chemistry and Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Biol Chem 277:1255-60. 2002..The differential affinity of MutS for DNA modified with the different platinum analogs could provide the molecular basis for the distinctive cellular responses to cisplatin and oxaliplatin... - YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella entericaSarah E Broadbent
Department of Biology, IIU, Area 12, University of York, P O Box 373, York Y010 5YW, England
J Bacteriol 189:4325-7. 2007..This study provides evidence that the E. coli yhdJ gene encodes a DNA adenine methyltransferase. In contrast to an earlier report, however, we show that yhdJ is not an essential gene in either E. coli or S. enterica... - Nitric oxide-induced homologous recombination in Escherichia coli is promoted by DNA glycosylasesErik J Spek
Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Bacteriol 184:3501-7. 2002..These studies shed light on the underlying mechanism of NO*-induced homologous recombination... - Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysisAnders Løbner-Olesen
Department of Life Sciences and Chemistry, Roskilde University, DK 4000 Roskilde, Denmark
Proc Natl Acad Sci U S A 100:4672-7. 2003..We suggest that the methylation status of the cell is an important factor in forming and/or maintaining chromosome structure...
Research Grants
- DNA Mismatch and Double-Strand Break RepairMartin Marinus; Fiscal Year: 2005..If these experiments support our model, it will open up a new approach to combat tumor cells by targeting recombination proteins. ..
- DNA Mismatch and Double-Strand Break RepairMartin Marinus; Fiscal Year: 2009..It also impacts the mechanism by which pathogenic bacteria become resistant to antibiotics and how this resistance is disseminated. ..
- DNA Mismatch and Double-Strand Break RepairMartin G Marinus; Fiscal Year: 2010..It also impacts the mechanism by which pathogenic bacteria become resistant to antibiotics and how this resistance is disseminated. ..