LYNNE MAQUAT

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. ncbi request reprint Molecular biology. Skiing toward nonstop mRNA decay
    Lynne E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 712, Rochester, NY 14642, USA
    Science 295:2221-2. 2002
  2. ncbi request reprint The dharma of nonsense-mediated mRNA decay in mammalian cells
    Maximilian Wei Lin Popp
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA Center for RNA Biology, University of Rochester, Rochester, New York 14642, USA
    Mol Cells 37:1-8. 2014
  3. pmc lncRNAs transactivate STAU1-mediated mRNA decay by duplexing with 3' UTRs via Alu elements
    Chenguang Gong
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    Nature 470:284-8. 2011
  4. doi request reprint mRNA-mRNA duplexes that autoelicit Staufen1-mediated mRNA decay
    Chenguang Gong
    1 Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York, USA 2 Center for RNA Biology, University of Rochester, Rochester, New York, USA 3
    Nat Struct Mol Biol 20:1214-20. 2013
  5. ncbi request reprint Organizing principles of mammalian nonsense-mediated mRNA decay
    Maximilian Wei Lin Popp
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642 email
    Annu Rev Genet 47:139-65. 2013
  6. pmc CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter
    L E Maquat
    Department of Biochemistry and Biophysics, The Center For RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    Cold Spring Harb Symp Quant Biol 75:127-34. 2010
  7. pmc The pioneer round of translation: features and functions
    Lynne E Maquat
    Department of Biochemistry and Biophysics and Center for RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
    Cell 142:368-74. 2010
  8. pmc Gene expression networks: competing mRNA decay pathways in mammalian cells
    Lynne E Maquat
    Department of Biochemistry and Biophysics and Center for RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
    Biochem Soc Trans 37:1287-92. 2009
  9. pmc Mammalian heat shock p70 and histone H4 transcripts, which derive from naturally intronless genes, are immune to nonsense-mediated decay
    L E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, New York 14642, USA
    RNA 7:445-56. 2001
  10. ncbi request reprint Nonsense-mediated mRNA decay in mammals
    Lynne E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    J Cell Sci 118:1773-6. 2005

Collaborators

  • H Sato
  • Woan Yuh Tarn
  • Nao Hosoda
  • Fred Sherman
  • B J Blencowe
  • Brendan J Frey
  • Ofer Shai
  • Robert Abraham
  • Yasuhito Ishigaki
  • X Li
  • Fabrice Lejeune
  • Yoon Ki Kim
  • Maximilian Wei Lin Popp
  • Chenguang Gong
  • Olaf Isken
  • Arneet L Saltzman
  • Qun Pan
  • Collynn F Woeller
  • Daiki Matsuda
  • Luc DesGroseillers
  • Luc Furic
  • Holly A Kuzmiak
  • Qinshan Gao
  • Aparna C Ranganathan
  • Shang Yi Chiu
  • Kathryn M Brumbaugh
  • Marco A Valencia-Sanchez
  • Xiaolei Sun
  • Audrey Stevens
  • Yalan Tang
  • Matthew M Fagnani
  • Greg L Mayeur
  • John W B Hershey
  • Martina Gaspari
  • Marc Parisien
  • Francois Major
  • Christine Misquitta
  • Biswadip Das
  • Randal S Tibbetts
  • Diane M Otterness
  • Vasco Oliveira
  • Christoph Geisen
  • John Brognard
  • Robert Hoepfner
  • Yang Wang
  • Kirsten Bremer
  • Daniel R Schoenberg
  • Michael Antoniou
  • Jing Zhang

Detail Information

Publications40

  1. ncbi request reprint Molecular biology. Skiing toward nonstop mRNA decay
    Lynne E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 712, Rochester, NY 14642, USA
    Science 295:2221-2. 2002
  2. ncbi request reprint The dharma of nonsense-mediated mRNA decay in mammalian cells
    Maximilian Wei Lin Popp
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA Center for RNA Biology, University of Rochester, Rochester, New York 14642, USA
    Mol Cells 37:1-8. 2014
    ..Here we review the mRNP rearrangements that culminate in detection and elimination of faulty transcripts by mammalian-cell NMD. ..
  3. pmc lncRNAs transactivate STAU1-mediated mRNA decay by duplexing with 3' UTRs via Alu elements
    Chenguang Gong
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    Nature 470:284-8. 2011
    ..We name these lncRNAs half-STAU1-binding site RNAs (1/2-sbsRNAs)...
  4. doi request reprint mRNA-mRNA duplexes that autoelicit Staufen1-mediated mRNA decay
    Chenguang Gong
    1 Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York, USA 2 Center for RNA Biology, University of Rochester, Rochester, New York, USA 3
    Nat Struct Mol Biol 20:1214-20. 2013
    ..If only one mRNA is translated, then it alone is targeted for SMD. We demonstrate the functional importance of mRNA-mRNA-triggered SMD in cell migration and invasion. ..
  5. ncbi request reprint Organizing principles of mammalian nonsense-mediated mRNA decay
    Maximilian Wei Lin Popp
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642 email
    Annu Rev Genet 47:139-65. 2013
    ....
  6. pmc CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter
    L E Maquat
    Department of Biochemistry and Biophysics, The Center For RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    Cold Spring Harb Symp Quant Biol 75:127-34. 2010
    ..Here, we overview cellular processes by which CBC-bound mRNPs are remodeled to eIF4E-bound mRNPs. We also describe the molecular movements of certain factors during NMD in view of the influential role of CBP80...
  7. pmc The pioneer round of translation: features and functions
    Lynne E Maquat
    Department of Biochemistry and Biophysics and Center for RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
    Cell 142:368-74. 2010
    ..Translation factors, RNA-binding proteins, and targets of signaling pathways that are particular to newly synthesized mRNAs regulate critical functions of the pioneer round...
  8. pmc Gene expression networks: competing mRNA decay pathways in mammalian cells
    Lynne E Maquat
    Department of Biochemistry and Biophysics and Center for RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
    Biochem Soc Trans 37:1287-92. 2009
    ..We describe how a factor that is common to both pathways results in their competition. We also explain how competition between the two pathways contributes to the differentiation of C2C12 myoblasts to multinucleated myotubes...
  9. pmc Mammalian heat shock p70 and histone H4 transcripts, which derive from naturally intronless genes, are immune to nonsense-mediated decay
    L E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, New York 14642, USA
    RNA 7:445-56. 2001
    ..Failure is most likely because each mRNA lacks a cis-acting destabilizing element, because insertion of a spliceable intron a sufficient distance downstream of a nonsense codon within either gene is sufficient to elicit NMD...
  10. ncbi request reprint Nonsense-mediated mRNA decay in mammals
    Lynne E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    J Cell Sci 118:1773-6. 2005
  11. ncbi request reprint Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics
    Lynne E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, New York 14642, USA
    Nat Rev Mol Cell Biol 5:89-99. 2004
    ..The acquisition and loss of mRNA-associated proteins accompanies the transition from the pioneer round to subsequent rounds of translation, and from translational competence to substrate for nonsense-mediated mRNA decay...
  12. ncbi request reprint Evidence that selenium deficiency results in the cytoplasmic decay of GPx1 mRNA dependent on pre-mRNA splicing proteins bound to the mRNA exon-exon junction
    L E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 712 Rochester, NY 14642, USA
    Biofactors 14:37-42. 2001
    ..Such evidence derives from the initial finding that GPx1 mRNA is a natural substrate of nonsense-mediated decay. Here, recent work on GPx1 RNA metabolism is reviewed and future directions of study are defined...
  13. ncbi request reprint Nonsense-mediated mRNA decay
    Lynne E Maquat
    Dept of Biochemistry and Biophysics, University of Rochester Medical Center, 601 Elmwood Avenue, Box 712, Rochester, New York 14642, USA
    Curr Biol 12:R196-7. 2002
  14. ncbi request reprint NASty effects on fibrillin pre-mRNA splicing: another case of ESE does it, but proposals for translation-dependent splice site choice live on
    Lynne E Maquat
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    Genes Dev 16:1743-53. 2002
  15. ncbi request reprint Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20
    Y Ishigaki
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 712, Rochester, NY 14642, USA
    Cell 106:607-17. 2001
    ..They also indicate that CBP80-bound mRNA undergoes a "pioneer" round of translation, before CBP80-CBP20 are replaced by eIF4E, and Upf2 and Upf3 proteins dissociate from upstream of exon-exon junctions...
  16. ncbi request reprint Immunopurification and analysis of protein and RNA components of mRNP in mammalian cells
    Fabrice Lejeune
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, NY, USA
    Methods Mol Biol 257:115-24. 2004
    ..We also describe a modification using beta-mercaptoethanol that facilitates analyzing proteins that comigrate with antibody light or heavy chains...
  17. ncbi request reprint Quality control of eukaryotic mRNA: safeguarding cells from abnormal mRNA function
    Olaf Isken
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    Genes Dev 21:1833-56. 2007
    ..Along with other types of quality control that occur during the complex processes of mRNA biogenesis, these mRNA surveillance mechanisms help to ensure the integrity of protein-encoding gene expression...
  18. ncbi request reprint Failsafe nonsense-mediated mRNA decay does not detectably target eIF4E-bound mRNA
    Daiki Matsuda
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 712, Rochester, New York 14642, USA
    Nat Struct Mol Biol 14:974-9. 2007
    ..Our studies, including analyses of factor dependence, reveal important shared features of the two mammalian-cell NMD pathways as well as fundamental differences between NMD in mammals and Saccharomyces cerevisiae...
  19. doi request reprint Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay
    Hanae Sato
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Mol Cell 29:255-62. 2008
    ..Our results uncover an additional role for SF2/ASF and indicate that the efficiency of the pioneer round of translation influences the efficiency of subsequent rounds of translation...
  20. pmc NMD resulting from encephalomyocarditis virus IRES-directed translation initiation seems to be restricted to CBP80/20-bound mRNA
    Collynn F Woeller
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 712, Rochester, New York 14642, USA
    EMBO Rep 9:446-51. 2008
    ..We show that EMCV IRES-initiated translation undergoes a CBP80/20-associated pioneer round of translation that results in CBP80/20-dependent and Upf factor-dependent NMD when translation terminates prematurely...
  21. doi request reprint Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay
    Olaf Isken
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    Cell 133:314-27. 2008
    ..Our results uncover a critical step during NMD that converts a pioneer translation initiation complex to a translationally compromised mRNP...
  22. pmc Regulation of multiple core spliceosomal proteins by alternative splicing-coupled nonsense-mediated mRNA decay
    Arneet L Saltzman
    Department of Molecular Genetics, Centre for Cellular and Biomolecular Research, 160 College Street, University of Toronto, Toronto, Ontario M5S 3E1, Canada
    Mol Cell Biol 28:4320-30. 2008
    ..The results further implicate general spliceosomal components in AS regulation...
  23. pmc The exon junction complex is detected on CBP80-bound but not eIF4E-bound mRNA in mammalian cells: dynamics of mRNP remodeling
    Fabrice Lejeune
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    EMBO J 21:3536-45. 2002
    ..Each of these proteins is also detected on CBP80-bound mRNA in the cytoplasmic fraction, indicating a presence on mRNA after export. The dynamics of mRNP composition before and after mRNA export are discussed...
  24. ncbi request reprint Mechanistic links between nonsense-mediated mRNA decay and pre-mRNA splicing in mammalian cells
    Fabrice Lejeune
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, New York, USA
    Curr Opin Cell Biol 17:309-15. 2005
    ....
  25. ncbi request reprint Applying nonsense-mediated mRNA decay research to the clinic: progress and challenges
    Holly A Kuzmiak
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    Trends Mol Med 12:306-16. 2006
    ..Here, we will review how to determine which PTCs elicit NMD, what is currently known about the mechanism of NMD, and additional information that is pertinent to establishing therapies for PTC-associated diseases...
  26. pmc The pioneer translation initiation complex is functionally distinct from but structurally overlaps with the steady-state translation initiation complex
    Shang Yi Chiu
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    Genes Dev 18:745-54. 2004
    ..Polysome profiles indicate that CBP80-bound mRNAs are translated less efficiently than their eIF4E-bound counterparts...
  27. ncbi request reprint Mammalian Staufen1 recruits Upf1 to specific mRNA 3'UTRs so as to elicit mRNA decay
    Yoon Ki Kim
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    Cell 120:195-208. 2005
    ..We discuss this pathway as a means for cells to downregulate the expression of Stau1 binding transcripts...
  28. pmc Cap-binding protein 1-mediated and eukaryotic translation initiation factor 4E-mediated pioneer rounds of translation in yeast
    Qinshan Gao
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 102:4258-63. 2005
    ..These results suggest that both Cbc1p- and eIF4E-mediated pioneer rounds of translation occur in yeast...
  29. ncbi request reprint CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells
    Nao Hosoda
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, New York 14642, USA
    Nat Struct Mol Biol 12:893-901. 2005
    ..SMD depends on the recruitment of Upf1 by the RNA-binding protein Stau1 but does not depend on the other Upf proteins. We find that CBP80 interacts with Upf1 and promotes the interaction of Upf1 with Upf2 but not with Stau1...
  30. pmc Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression
    Qun Pan
    Banting and Best Department of Medical Research, University of Toronto, Ontario, M5G 1L6, Canada
    Genes Dev 20:153-8. 2006
    ..Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles...
  31. pmc Evidence that poly(A) binding protein C1 binds nuclear pre-mRNA poly(A) tails
    Nao Hosoda
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, Rochester, NY 14642
    Mol Cell Biol 26:3085-97. 2006
    ..Our results are discussed in the context of pre-mRNA processing and stability and mRNA trafficking and the pioneer round of translation...
  32. ncbi request reprint eIF4G is required for the pioneer round of translation in mammalian cells
    Fabrice Lejeune
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, New York, 14642 USA
    Nat Struct Mol Biol 11:992-1000. 2004
    ..We propose a model in which eIF4G serves to connect CBP80/20 with other initiation factors during the pioneer round of translation...
  33. ncbi request reprint Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities
    Fabrice Lejeune
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 712, Rochester, NY 14642, USA
    Mol Cell 12:675-87. 2003
    ..From these and other data, we conclude that NMD in mammalian cells degrades mRNAs from both 5' and 3' ends by recruiting decapping and 5'-->3' exonuclease activities as well as deadenylating and 3'-->5' exonuclease activities...
  34. ncbi request reprint Nonsense-mediated decay: assaying for effects on selenoprotein mRNAs
    Xiaolei Sun
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    Methods Enzymol 347:49-57. 2002
    ..This assay is simpler and more versatile than the first assay because it can be used to assay any cellular gene in situ provided the cells can be stably transfected with the hUpf1p expression vector...
  35. ncbi request reprint The mRNA surveillance protein hSMG-1 functions in genotoxic stress response pathways in mammalian cells
    Kathryn M Brumbaugh
    Program in Signal Transduction Research, The Burnham Institute, La Jolla, CA 92037, USA
    Mol Cell 14:585-98. 2004
    ..Finally, NMD is suppressed in hSMG-1- but not ATM-deficient cells. These results indicate that hSMG-1 plays important roles in the maintenance of both genome and transcriptome integrity in human cells...
  36. ncbi request reprint RNA-protein interactions: insight into gene function
    Lynne E Maquat
    Methods 26:93-4. 2002
  37. ncbi request reprint An enemy within: fly reconnaissance deploys an endonuclease to destroy nonsense-containing mRNA
    Marco A Valencia-Sanchez
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, 601 Elmwood Avenue, Box 712, University of Rochester, Rochester, NY 14642, USA
    Trends Cell Biol 14:594-7. 2004
    ..Although NMD is conserved in all eukaryotes that have been examined, it can manifest mechanistic differences in different organisms. A recent study using Drosophila melanogaster describes a new mechanistic twist to NMD...
  38. pmc Beta -Globin mRNA decay in erythroid cells: UG site-preferred endonucleolytic cleavage that is augmented by a premature termination codon
    Audrey Stevens
    Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
    Proc Natl Acad Sci U S A 99:12741-6. 2002
    ..These data suggest that an endonuclease with preference for UG dinucleotides is involved in the degradation of nonsense-containing and, to a lesser extent, nonsense-free human beta-globin mRNAs in mouse erythroid cells...
  39. pmc Staufen1 regulates diverse classes of mammalian transcripts
    Yoon Ki Kim
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
    EMBO J 26:2670-81. 2007
    ..We propose that Stau1 influences the expression of a wide variety of physiologic transcripts and metabolic pathways...

Research Grants27

  1. Nonsense-mediated mRNA decay: Pioneer round of translation
    Lynne E Maquat; Fiscal Year: 2010
    ..We expect that renewed support of this grant will allow us to continue to make major advances in understanding the mechanism of NMD in mammalian cells. ..
  2. Nonsense-mediated mRNA decay: Pioneer round of translation
    LYNNE MAQUAT; Fiscal Year: 2009
    ..We expect that renewed support of this grant will allow us to continue to make major advances in understanding the mechanism of NMD in mammalian cells. ..
  3. REGULATION OF SELENOPROTEIN RNA METABOLISM
    LYNNE MAQUAT; Fiscal Year: 2001
    ..2. Determine why premature termination of PHGPx mRNA translation at the position of the Sec codon fails to mediate a significant reduction in PHGPx mRNA abundance. ..
  4. Nonsence-mediated mRNA decay: Pioneer round of translation
    LYNNE MAQUAT; Fiscal Year: 2007
    ..We expect that renewed support of this grant will allow us to continue to make major advances in understanding the mechanism of NMD in mammalian cells. ..
  5. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2002
    ..elegans. Characterize the human homologue of Ubf1 and its role in NMD, in particular studying the role of its ATPase and putative helicase activities, and what cellular proteins it interacts with. ..
  6. REGULATION OF SELENOPROTEIN RNA METABOLISM
    LYNNE MAQUAT; Fiscal Year: 2000
    ..2. Determine why premature termination of PHGPx mRNA translation at the position of the Sec codon fails to mediate a significant reduction in PHGPx mRNA abundance. ..
  7. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2003
    ..elegans. Characterize the human homologue of Ubf1 and its role in NMD, in particular studying the role of its ATPase and putative helicase activities, and what cellular proteins it interacts with. ..
  8. Nonsense-mediated mRNA decay:pioneer translation round
    LYNNE MAQUAT; Fiscal Year: 2003
    ..abstract_text> ..
  9. Training in Cellular, Biochemical and Molecular Sciences
    LYNNE MAQUAT; Fiscal Year: 2007
    ..This program has greatly promoted scientific interactions among laboratories, increased flexibility in the choice of research mentors, and improved graduate recruiting. ..
  10. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2005
    ..elegans. Characterize the human homologue of Ubf1 and its role in NMD, in particular studying the role of its ATPase and putative helicase activities, and what cellular proteins it interacts with. ..
  11. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2006
    ..The proposed experiments logically extend our long-time studies, and they should lend important insight into aspects of SMD and NMD that have yet to be understood. ..
  12. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    Lynne E Maquat; Fiscal Year: 2010
    ..As an example, NMD provides a means by which many RNA binding proteins negatively regulate the genes from which they derived so that an appropriate level of gene expression can be achieved. ..
  13. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2001
    ..elegans. Characterize the human homologue of Ubf1 and its role in NMD, in particular studying the role of its ATPase and putative helicase activities, and what cellular proteins it interacts with. ..
  14. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    Lynne E Maquat; Fiscal Year: 2011
    ..As an example, NMD provides a means by which many RNA binding proteins negatively regulate the genes from which they derived so that an appropriate level of gene expression can be achieved. ..
  15. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2009
    ..The proposed experiments logically extend our long-time studies, and they should lend important insight into aspects of SMD and NMD that have yet to be understood. ..
  16. Nonsence-mediated mRNA decay: Pioneer round of translation
    LYNNE MAQUAT; Fiscal Year: 2009
    ..We expect that renewed support of this grant will allow us to continue to make major advances in understanding the mechanism of NMD in mammalian cells. ..
  17. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2007
    ..The proposed experiments logically extend our long-time studies, and they should lend important insight into aspects of SMD and NMD that have yet to be understood. ..
  18. Nonsense-mediated mRNA decay:pioneer translation round
    LYNNE MAQUAT; Fiscal Year: 2006
    ..abstract_text> ..
  19. Nonsense-mediated mRNA decay:pioneer translation round
    LYNNE MAQUAT; Fiscal Year: 2005
    ..abstract_text> ..
  20. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2004
    ..elegans. Characterize the human homologue of Ubf1 and its role in NMD, in particular studying the role of its ATPase and putative helicase activities, and what cellular proteins it interacts with. ..
  21. Nonsense-mediated mRNA decay:pioneer translation round
    LYNNE MAQUAT; Fiscal Year: 2004
    ..abstract_text> ..
  22. REGULATION OF SELENOPROTEIN RNA METABOLISM
    LYNNE MAQUAT; Fiscal Year: 2002
    ..2. Determine why premature termination of PHGPx mRNA translation at the position of the Sec codon fails to mediate a significant reduction in PHGPx mRNA abundance. ..
  23. POST-TRANSCRIPTIONAL CONTROL OF GENE EXPRESSION
    LYNNE MAQUAT; Fiscal Year: 2002
    ....
  24. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    LYNNE MAQUAT; Fiscal Year: 2000
    ..elegans. Characterize the human homologue of Ubf1 and its role in NMD, in particular studying the role of its ATPase and putative helicase activities, and what cellular proteins it interacts with. ..