Kenneth G Mann

Summary

Affiliation: University of Vermont
Country: USA

Publications

  1. ncbi request reprint Thrombin formation
    Kenneth G Mann
    Department of Biochemistry, University of Vermont College of Medicine, Burlington, VT 05405, USA
    Chest 124:4S-10S. 2003
  2. pmc From principle to practice: bridging the gap in patient profiling
    Jonathan H Foley
    Department of Biochemistry, University of Vermont, Burlington, Vermont, United States of America
    PLoS ONE 8:e54728. 2013
  3. pmc Membrane binding events in the initiation and propagation phases of tissue factor-initiated zymogen activation under flow
    Laura M Haynes
    Department of Biochemistry, University of Vermont College of Medicine, Colchester, Vermont 05446, USA
    J Biol Chem 287:5225-34. 2012
  4. pmc Disulfide reduction abolishes tissue factor cofactor function
    Jolanta Krudysz-Amblo
    Department of Biochemistry, University of Vermont, Colchester, VT 05446, USA
    Biochim Biophys Acta 1830:3489-96. 2013
  5. pmc Posttranslational modifications and activity of natural and recombinant tissue factor
    Saulius Butenas
    Department of Biochemistry, University of Vermont, 208 South Park Drive, Burlington, VT 05446, USA
    Thromb Res 125:S26-8. 2010
  6. pmc The prothrombotic phenotypes in familial protein C deficiency are differentiated by computational modeling of thrombin generation
    Kathleen E Brummel-Ziedins
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, Vermont, United States of America
    PLoS ONE 7:e44378. 2012
  7. pmc Prothrombin activation by platelet-associated prothrombinase proceeds through the prethrombin-2 pathway via a concerted mechanism
    Laura M Haynes
    Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont 05405, USA
    J Biol Chem 287:38647-55. 2012
  8. pmc The impact of uncertainty in a blood coagulation model
    Christopher M Danforth
    Department of Mathematics and Statistics, Center for Complex Systems, Vermont Advanced Computing Center, University of Vermont, Burlington, VT 05401, USA
    Math Med Biol 26:323-36. 2009
  9. pmc Rivaroxaban delivery and reversal at a venous flow rate
    Laura M Haynes
    Department of Biochemistry, University of Vermont College of Medicine, Colchester, VT 05446, USA
    Arterioscler Thromb Vasc Biol 32:2877-83. 2012
  10. pmc Modeling of human factor Va inactivation by activated protein C
    MARIA CRISTINA BRAVO
    Cell and Molecular Biology Program, University of Vermont, 89 Beaumont Ave, Burlington, VT 05405, USA
    BMC Syst Biol 6:45. 2012

Collaborators

Detail Information

Publications58

  1. ncbi request reprint Thrombin formation
    Kenneth G Mann
    Department of Biochemistry, University of Vermont College of Medicine, Burlington, VT 05405, USA
    Chest 124:4S-10S. 2003
    ..This review provides a brief summary of the evolution of knowledge with respect to present-day concepts of thrombin generation via the tissue factor pathway and its regulation...
  2. pmc From principle to practice: bridging the gap in patient profiling
    Jonathan H Foley
    Department of Biochemistry, University of Vermont, Burlington, Vermont, United States of America
    PLoS ONE 8:e54728. 2013
    ..Ultimately, our method is designed to visualize individualized patient profiles which are becoming evermore important as personalized medicine strategies become routine clinical practice...
  3. pmc Membrane binding events in the initiation and propagation phases of tissue factor-initiated zymogen activation under flow
    Laura M Haynes
    Department of Biochemistry, University of Vermont College of Medicine, Colchester, Vermont 05446, USA
    J Biol Chem 287:5225-34. 2012
    ....
  4. pmc Disulfide reduction abolishes tissue factor cofactor function
    Jolanta Krudysz-Amblo
    Department of Biochemistry, University of Vermont, Colchester, VT 05446, USA
    Biochim Biophys Acta 1830:3489-96. 2013
    ..The conclusions of published studies on this subject are based on indirect evidences obtained by the use of reagents with potentially oxidizing/reducing properties...
  5. pmc Posttranslational modifications and activity of natural and recombinant tissue factor
    Saulius Butenas
    Department of Biochemistry, University of Vermont, 208 South Park Drive, Burlington, VT 05446, USA
    Thromb Res 125:S26-8. 2010
    ..Thus the use of recombinant tissue factor proteins for the quantitation of natural tissue factor is misleading...
  6. pmc The prothrombotic phenotypes in familial protein C deficiency are differentiated by computational modeling of thrombin generation
    Kathleen E Brummel-Ziedins
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, Vermont, United States of America
    PLoS ONE 7:e44378. 2012
    ..In addition, variations within the plasma composition of each individual can further segregate out increased procoagulant phenotypes, with gender-associated plasma compositional differences playing a large role...
  7. pmc Prothrombin activation by platelet-associated prothrombinase proceeds through the prethrombin-2 pathway via a concerted mechanism
    Laura M Haynes
    Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont 05405, USA
    J Biol Chem 287:38647-55. 2012
    ..Collectively, these findings suggest that platelet-associated prothrombinase activates prothrombin via an efficient concerted mechanism in which neither intermediate is released...
  8. pmc The impact of uncertainty in a blood coagulation model
    Christopher M Danforth
    Department of Mathematics and Statistics, Center for Complex Systems, Vermont Advanced Computing Center, University of Vermont, Burlington, VT 05401, USA
    Math Med Biol 26:323-36. 2009
    ....
  9. pmc Rivaroxaban delivery and reversal at a venous flow rate
    Laura M Haynes
    Department of Biochemistry, University of Vermont College of Medicine, Colchester, VT 05446, USA
    Arterioscler Thromb Vasc Biol 32:2877-83. 2012
    ....
  10. pmc Modeling of human factor Va inactivation by activated protein C
    MARIA CRISTINA BRAVO
    Cell and Molecular Biology Program, University of Vermont, 89 Beaumont Ave, Burlington, VT 05405, USA
    BMC Syst Biol 6:45. 2012
    ..An empirically validated mathematical model of this process would be useful in advancing the predictive capacity of comprehensive models of coagulation...
  11. ncbi request reprint Influence of bivalirudin on tissue factor-triggered coagulation
    Saulius Butenas
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, Vermont, USA
    Blood Coagul Fibrinolysis 18:407-14. 2007
    ..In conclusion, bivalirudin at pharmacologic concentrations is an efficient inhibitor of thrombin generation, platelet activation and clot formation, which acts not as a modulator but as an 'on-off' switch of blood coagulation...
  12. ncbi request reprint Tissue factor in thrombosis and hemorrhage
    Saulius Butenas
    Department of Biochemistry, University of Vermont, Colchester, VT 05446, USA
    Surgery 142:S2-14. 2007
    ....
  13. pmc Platelets do not express the oxidized or reduced forms of tissue factor
    Beth A Bouchard
    Department of Biochemistry, University of Vermont College of Medicine, Burlington, VT, USA
    Biochim Biophys Acta 1840:1188-93. 2014
    ..This study tests the hypothesis that the discrepancies regarding platelet TF expression are due to differential expression of the two forms...
  14. pmc Active tissue factor and activated factor XI in patients with acute ischemic cerebrovascular events
    Anetta Undas
    Institute of Cardiology, Jagiellonian University Medical College, Krakow, Poland
    Eur J Clin Invest 42:123-9. 2012
    ..We sought to investigate whether circulating activated FXI (FXIa) and TF on admission can predict clinical outcomes in patients with acute cerebrovascular events...
  15. pmc Differences in the fractional abundances of carbohydrates of natural and recombinant human tissue factor
    Jolanta Krudysz-Amblo
    Department of Biochemistry, University of Vermont, Burlington, VT 05405, USA
    Biochim Biophys Acta 1810:398-405. 2011
    ..Previously we have shown that the activity of the natural placental TF (pTF) and the recombinant TF (rTF) from Sf9 insect cells is different (Krudysz-Amblo, J. et al (2010) J. Biol. Chem. 285, 3371-3382)...
  16. pmc The nature of the stable blood clot procoagulant activities
    Thomas Orfeo
    Department of Biochemistry, University of Vermont, Colchester, VT 05446, USA
    J Biol Chem 283:9776-86. 2008
    ..e. hemophilias A and B, result in an impaired capacity to mount a resupply response; and 3) in normal hemostasis the intrinsic factor Xase function contributes to the durability of the resupply response...
  17. pmc Carbohydrates and activity of natural and recombinant tissue factor
    Jolanta Krudysz-Amblo
    Department of Biochemistry, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 285:3371-82. 2010
    ..The carbohydrates of rTF(1-263) contain high mannose, hybrid, and fucosylated glycans. Natural pTF contains no high mannose glycans but is modified with hybrid, highly fucosylated, and sialylated sugars...
  18. pmc Prothrombin activation in blood coagulation: the erythrocyte contribution to thrombin generation
    Matthew F Whelihan
    Department of Biochemistry, University of Vermont, Burlington, VT, USA
    Blood 120:3837-45. 2012
    ..These data indicate that RBCs participate in thrombin generation in Tf-activated blood, producing a membrane that supports prothrombin activation through the meizothrombin pathway...
  19. pmc Plasma factor and inhibitor composition contributes to thrombin generation dynamics in patients with acute or previous cerebrovascular events
    Matthew Gissel
    Department of Biochemistry, University of Vermont, Colchester, VT, USA
    Thromb Res 126:262-9. 2010
    ..We evaluated whether plasma factor composition and thrombin generation dynamics might be a contributor to the thrombotic phenotype of ischemic cerebrovascular events...
  20. pmc Tissue factor in coagulation: Which? Where? When?
    Saulius Butenas
    Department of Biochemistry, University of Vermont, 208 South Park Drive, Suite 2, Room 235A, Colchester, VT 05446, USA
    Arterioscler Thromb Vasc Biol 29:1989-96. 2009
    ..We also highlight controversies concerning the expression/presence of TF on various cells and in blood in normal and pathological states...
  21. ncbi request reprint Thrombin functions during tissue factor-induced blood coagulation
    Kathleen E Brummel
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, VT 05405, USA
    Blood 100:148-52. 2002
    ..6 +/- 0.6 minutes). These results illustrate that the initial activation of thrombin substrates occurs during the initiation phase at less than 2 nM thrombin (0.2%). Most thrombin (96%) is formed well after clotting occurs...
  22. pmc Quantitation of anti-factor VIII antibodies in human plasma
    Jolanta Krudysz-Amblo
    Department of Biochemistry, College of Medicine, University of Vermont, Burlington, VT, USA
    Blood 113:2587-94. 2009
    ..6 to 6.2 nM, whereas 13 (33%) of the 39 inhibitor-free hemophilia A subjects were positive for anti-fVIIIAb in the range of 0.5 to 20 nM. The method may be useful for therapeutic management of hemophilia A patients...
  23. ncbi request reprint Models of blood coagulation
    Kenneth G Mann
    Department of Biochemistry, 208 South Park Drive, Suite 2, University of Vermont, College of Medicine, Colchester, VT 05446, USA
    Blood Cells Mol Dis 36:108-17. 2006
    ..Ideally, our results will provide descriptions which predict the behavior of the biological blood coagulation system under normal and pathologic conditions...
  24. pmc Dilutional control of prothrombin activation at physiologically relevant shear rates
    Laura M Haynes
    Department of Biochemistry, University of Vermont, College of Medicine, Colchester, Vermont, USA
    Biophys J 100:765-73. 2011
    ....
  25. pmc The "normal" factor VIII concentration in plasma
    Saulius Butenas
    Department of Biochemistry, College of Medicine, University of Vermont, Burlington, VT 05446, USA
    Thromb Res 126:119-23. 2010
    ..The quantitation of factor (F)VIII by activity-based assays is influenced by the method, procedure, the quality and properties of reagents used and concentrations of other plasma proteins, including von Willebrand factor (VWF)...
  26. ncbi request reprint The factor V activation paradox
    Thomas Orfeo
    Department of Biochemistry, University of Vermont, 89 Beaumont Avenue, Burlington, VT 05405 0068, USA
    J Biol Chem 279:19580-91. 2004
    ..Direct activation of factor V by factor Xa at physiologically relevant concentrations does not appear to be a significant contributor to factor Va formation...
  27. ncbi request reprint Mechanism of factor VIIa-dependent coagulation in hemophilia blood
    Saulius Butenas
    College of Medicine, University of Vermont, 89 Beaumont Ave, Burlington, VT 05405 0068, USA
    Blood 99:923-30. 2002
    ..Thus, pharmacologic concentrations of factor VIIa cannot restore normal thrombin generation in hemophilia A and hemophilia B blood in vitro. The efficacy of factor VIIa (10-50 nM) in hemophilia blood is dependent on TF...
  28. pmc Tissue factor controversies
    Kenneth G Mann
    University of Vermont, Department of Biochemistry, Burlington, VT, corrected USA
    Thromb Res 129:S5-7. 2012
    ..This presentation will review data on the architecture and functions of the tissue factor-factor VIIa complex and discuss the elements of the controversies associated with tissue factor presentation in both normal and pathologic milieu...
  29. pmc Cellular regulation of blood coagulation: a model for venous stasis
    James E Campbell
    Department of Biochemistry, University of Vermont, Colchester, VT 05446, USA
    Blood 116:6082-91. 2010
    ....
  30. doi request reprint Factor XIa and tissue factor activity in patients with coronary artery disease
    Saulius Butenas
    University of Vermont, Department of Biochemistry, Burlington, Vermont, USA
    Thromb Haemost 99:142-9. 2008
    ..59 and 0.39, respectively) and between FXIa and TAT (R(2) = 0.64 and 0.63, respectively). In conclusion, the majority of ACS and CAD-MI patients have circulating FXIa that correlates with markers of coagulation and inflammation...
  31. doi request reprint Tissue factor activity and function in blood coagulation
    Saulius Butenas
    Department of Biochemistry, University of Vermont, Burlington, VT, USA
    Thromb Res 122:S42-6. 2008
    ....
  32. pmc The tissue factor requirement in blood coagulation
    Thomas Orfeo
    Department of Biochemistry, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 280:42887-96. 2005
    ..These observations support the hypothesis that the transient, localized expression of TF is sufficient to sustain a TF-independent procoagulant response as long as flow persists...
  33. pmc Taking the thrombin "fork"
    Kenneth G Mann
    University of Vermont, College of Medicine, 208 South Park Drive, Colchester, VT 05446, USA
    Arterioscler Thromb Vasc Biol 30:1293-9. 2010
    ..take it." My career is a consequence of chance interactions with great mentors and talented students and the opportunities provided by a succession of ground-breaking improvements in technology...
  34. ncbi request reprint Thrombin: can't live without it; probably die from it
    Kenneth G Mann
    Department of Biochemistry, University of Vermont College of Medicine, Burlington, VT 05405, USA
    Chest 124:1S-3S. 2003
  35. ncbi request reprint Influence of factor VIIa and phospholipids on coagulation in "acquired" hemophilia
    Saulius Butenas
    University of Vermont, College of Medicine, Burlington, VT 05405 0068, USA
    Arterioscler Thromb Vasc Biol 23:123-9. 2003
    ..This study was performed to evaluate the influences of phospholipids and recombinant factor VIIa (rFVIIa) on thrombin generation and clot formation in "acquired" hemophilia B...
  36. ncbi request reprint The dynamics of thrombin formation
    Kenneth G Mann
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, VT 05405, USA
    Arterioscler Thromb Vasc Biol 23:17-25. 2003
    ....
  37. ncbi request reprint Factor V: a combination of Dr Jekyll and Mr Hyde
    Kenneth G Mann
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405, USA
    Blood 101:20-30. 2003
  38. ncbi request reprint Tissue factor activity in whole blood
    Saulius Butenas
    University of Vermont, Department of Biochemistry, Given Building, 89 Beaumont Ave, Burlington, VT 05405 0068, USA
    Blood 105:2764-70. 2005
    ..Our data indicate that the concentration of physiologically active TF in non-cytokine-stimulated blood from healthy individuals cannot exceed and is probably lower than 20 fM...
  39. ncbi request reprint The role of the red cell membrane in thrombin generation
    Matthew F Whelihan
    Department of Biochemistry, University of Vermont, Burlington, VT 05446, USA
    Thromb Res 131:377-82. 2013
    ....
  40. doi request reprint Modeling thrombin generation: plasma composition based approach
    Kathleen E Brummel-Ziedins
    Department of Biochemistry, College of Medicine, University of Vermont, Burlington, VT, USA
    J Thromb Thrombolysis 37:32-44. 2014
    ....
  41. pmc Posttranslational modifications of tissue factor
    Saulius Butenas
    Department of Biochemistry, College of Medicine, University of Vermont, Burlington, VT 05446, USA
    Front Biosci (Elite Ed) 4:381-91. 2012
    ..Overall, it is clear that some modifications of TF have an effect on TF procoagulant activity, signaling functions and trafficking. The influences of other modifications are debatable...
  42. ncbi request reprint The resuscitative fluid you choose may potentiate bleeding
    Kathleen Brummel-Ziedins
    Department of Biochemistry, School of Medicine, University of Vermont, Burlington, Vermont 05405, USA
    J Trauma 61:1350-8. 2006
    ..Little is known about how various resuscitative paradigms affect the coagulation cascade, which is essential to controlling hemorrhagic shock...
  43. ncbi request reprint A model for the stoichiometric regulation of blood coagulation
    Matthew F Hockin
    Department of Biochemistry, College of Medicine, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 277:18322-33. 2002
    ..A comparison of the model with empirical laboratory data illustrates that most experimentally observable parameters are captured, and the pathology that results in enhanced or deficient thrombin generation is accurately described...
  44. ncbi request reprint The challenge of regulating anticoagulant drugs: focus on warfarin
    Kenneth G Mann
    Vermont College of Medicine, Department of Biochemistry, University of Vermont, Burlington, VT 05405, USA
    Am Heart J 149:S36-42. 2005
  45. pmc The crystal structure of activated protein C-inactivated bovine factor Va: Implications for cofactor function
    Ty E Adams
    Department of Biochemistry, College of Medicine, University of Vermont, 89 Beaumont Avenue, Burlington, VT 05405, USA
    Proc Natl Acad Sci U S A 101:8918-23. 2004
    ..This structure represents the largest physiologically relevant fragment of factor Va solved to date and provides a new scaffold for the future generation of models of coagulation cofactors...
  46. ncbi request reprint The significance of circulating factor IXa in blood
    Saulius Butenas
    Department of Biochemistry, University of Vermont, Burlington, Vermont 05405 0068, USA
    J Biol Chem 279:22875-82. 2004
    ....
  47. pmc Intracellular and surface distribution of monocyte tissue factor: application to intersubject variability
    Elena M Egorina
    Department of Biochemistry, Institute of Medical Biology, University of Tromsø, Tromsø, Norway
    Arterioscler Thromb Vasc Biol 25:1493-8. 2005
    ..We characterized patterns of intracellular accumulation, externalization, and shedding of TF in response to LPS in mononuclear cells (MNCs) from high responders (HRs) and low responders (LRs)...
  48. pmc Simvastatin given for 3 days can inhibit thrombin generation and activation of factor V and enhance factor Va inactivation in hypercholesterolemic patients
    Anetta Undas
    Arterioscler Thromb Vasc Biol 25:1524-5. 2005
  49. ncbi request reprint Statins and blood coagulation
    Anetta Undas
    Department of Medicine, Jagiellonian University School of Medicine, Krakow, Poland
    Arterioscler Thromb Vasc Biol 25:287-94. 2005
    ..Treatment with statins can lead to a significant downregulation of the blood coagulation cascade, most probably as a result of decreased tissue factor expression, which leads to reduced thrombin generation...
  50. ncbi request reprint Blood coagulation at the site of microvascular injury in healthy and coumadin-treated subjects heterogenous for factor V Leiden mutation
    Anetta Undas
    Department of Medicine, Jagiellonian University School of Medicine, Crakow, Poland
    Thromb Haemost 98:1024-30. 2007
    ..Inactivation of FVa by the APC mechanism is attenuated significantly in carriers of FV Leiden but the magnitude of this effect is smaller than that observed in most purified systems...
  51. ncbi request reprint Structural requirements for expression of factor Va activity
    Michael Kalafatis
    Department of Chemistry, Cleveland State University, and The Lerner Research Institute, The Cleveland Clinic Foundation, Ohio, USA
    J Biol Chem 278:33550-61. 2003
    ....
  52. ncbi request reprint A review of the therapeutic uses of thrombin
    Roger L Lundblad
    Department of Pathology, University of North Carolina at Chapel Hill, USA
    Thromb Haemost 91:851-60. 2004
    ..However, the availability of a safe human thrombin preparation will be critical for the continued use of thrombin as a therapeutic...
  53. ncbi request reprint Identification of an inactivating cleavage site for alpha-thrombin on the heavy chain of factor Va
    Evrim Erdogan
    Department of Chemistry, Cleveland State University, 2351 Euclid Avenue, Science and Research Center SR 370, Cleveland, Ohio 44115, USA
    Thromb Haemost 98:998-1006. 2007
    ..Our data demonstrate that cleavage of FVa at Arg(643) by alpha-thrombin results in a partially inactive cofactor molecule and provides for an activated protein C (APC)-independent anticoagulant effect of alpha-thrombin...
  54. ncbi request reprint A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa
    Richard J Jenny
    Haematologic Technologies, Inc, Essex Junction, VT, USA
    Protein Expr Purif 31:1-11. 2003
    ..Examples are presented which describe the proteolysis of the protein of interest by either factor Xa or thrombin...
  55. ncbi request reprint Active tissue factor in blood?
    Saulius Butenas
    Nat Med 10:1155-6; author reply 1156. 2004
  56. ncbi request reprint Factor VII-activating protease: coagulation, fibrinolysis, and atherothrombosis?
    Kenneth G Mann
    Circulation 107:654-5. 2003
  57. pmc Antithrombotic properties of aspirin and resistance to aspirin: beyond strictly antiplatelet actions
    Anetta Undas
    Institute of Cardiology, Jagiellonian University School of Medicine, Krakow, Poland
    Blood 109:2285-92. 2007
    ..Elucidation of the actual impacts of aspirin other than antiaggregation effects could be important in view of the widespread use of this drug in the prevention of thrombotic manifestations of atherosclerosis...
  58. ncbi request reprint Aspirin alters the cardioprotective effects of the factor XIII Val34Leu polymorphism
    Anetta Undas
    Department of Medicine, Jagiellonian University School of Medicine, Cracow, Poland
    Circulation 107:17-20. 2003
    ....