Research Topics
Species | James MandellSummaryAffiliation: University of Virginia Country: USA Publications
Research Grants
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Detail Information
Publications
Juxtamembrane localization of the protein phosphatase-1 inhibitor protein PHI-1 in smooth muscle cellsNikolaos A Tountas
Center for Cell Signaling, University of Virginia School of Medicine, PO Box 800577, Charlottesville, VA 22908, USA
Histochem Cell Biol 121:343-50. 2004..The common tissue distribution but different cellular localization of PHI-1 and CPI-17 suggest distinctive physiological roles for these two PP1 inhibitors...- Primary disseminated leptomeningeal oligodendroglioma with 1p deletion. Case reportT David Bourne
Department of Pathology, Division of Neuropathology, University of Virginia Health System, Charlottesville, Virginia 22908-0214, USA
J Neurosurg 105:465-9. 2006..The deletion of lp was demonstrated by FISH analysis; lq, 19p, and 19q were intact. This appears to be the first reported case of a primary diffuse leptomeningeal oligodendroglioma in which a lp deletion was identified... 
In situ visualization of intratumor growth factor signaling: immunohistochemical localization of activated ERK/MAP kinase in glial neoplasmsJ W Mandell
Department of Pathology Neuropathology, University of Virginia School of Medicine, Charlottesville 22908, USA
Am J Pathol 153:1411-23. 1998..Although ERK/MAPK activation was not restricted to neoplastic glia, consistent patterns of selective activation in tumor cells suggests that sustained activation may contribute to the neoplastic glial phenotype...- A green fluorescent protein kinase substrate allowing detection and localization of intracellular ERK/MAP kinase activityJ W Mandell
Department of Pathology Neuropathology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
Anal Biochem 293:264-8. 2001..The approach described should be generally applicable to the intracellular analysis of many specific protein kinase substrates for which phospho-specific antibodies have been produced... 
Dephosphorylation of beta-arrestin 1 in glioblastomasJames W Mandell
Department of Pathology, University of Virginia Health System, Charlottesville, VA 22903, USA
J Neuropathol Exp Neurol 68:535-41. 2009..These results suggest that beta-arrestin 1 dephosphorylation/inactivation is associated with aspects of the malignant behavior of glioblastomas...- Immunohistochemical assessment of protein phosphorylation state: the dream and the realityJames W Mandell
Department of Pathology, University of Virginia School of Medicine, PO Box 800904, Charlottesville, VA 22908, USA
Histochem Cell Biol 130:465-71. 2008..The next phase of translational research in applications of PSSAs will entail the hard work of antibody validation, gathering of detailed information about epitope-specific lability, and implementation of methods for standardization... - Phosphorylation state-specific antibodies: applications in investigative and diagnostic pathologyJames W Mandell
Department of Pathology Neuropathology and Neuroscience Graduate Program, University of Virginia, Charlottesville, VA 22908, USA
Am J Pathol 163:1687-98. 2003..Novel applications of PSSAs are also beginning to reveal molecular mechanisms of inflammatory, degenerative, and toxin-induced diseases... - Follicular lymphoma of the dura: case reportD Kojo Hamilton
Department of Neurological Surgery, University of Virginia, Charlottesville 22908, USA
Neurosurgery 59:E703-4; discussion E703-4. 2006..The case illustrates the clinical, radiological, and histological relevance of this rare entity... - WT1 is not a reliable marker to distinguish reactive from neoplastic astrocyte populations in the central nervous systemT David Bourne
Department of Pathology Neuropathology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
Brain Pathol 20:1090-5. 2010..Thus, our findings indicate that WT1 expression alone is not a reliable feature to distinguish reactive from neoplastic astrocytes... - A pharmacological activator of AMP-activated protein kinase (AMPK) induces astrocyte stellationCarlita B Favero
Neuroscience Graduate Program, University of Virginia, Charlottesville, VA 22908, USA
Brain Res 1168:1-10. 2007..Our findings suggest that AMPK mediates morphological alterations of astrocytes in response to energy depletion... - Fluorescence imaging of mobility shifts: an expression cloning method for identification of cell signaling targetsJames W Mandell
Department of Pathology, University of Virginia, Charlottesville, Virginia 22908, USA
Lab Invest 82:1631-6. 2002..We illustrate the utility of the technique with pilot screens for apoptotic and mitogenic targets modified by staurosporine and serum stimulation, respectively... - Serotonergic dystrophy induced by excess serotoninElizabeth A Daubert
Neuroscience Graduate Program, University of Virginia, Charlottesville, VA 22908, USA
Mol Cell Neurosci 44:297-306. 2010..The data presented here support a model in which excess cytoplasmic neurotransmitter triggers a cell-specific pathway inducing aberrant morphology in fly serotonergic neurons that may be shared in certain mammalian pathologies... - Transient neuronal but persistent astroglial activation of ERK/MAP kinase after focal brain injury in miceW Shawn Carbonell
Medical Scientist Training Program, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
J Neurotrauma 20:327-36. 2003..Sustained activation of the ERK/MAPK signaling pathway in perilesional reactive glia suggests a critical role for this cascade in astrogliosis... - SIMPLE: a sequential immunoperoxidase labeling and erasing methodGeorge Glass
University of Virginia, UVA Health System, Charlottesville, VA 22908, USA
J Histochem Cytochem 57:899-905. 2009..SIMPLE is greatly facilitated by the use of a whole-slide scanner, which can capture the results of each sequential stain without any information loss... - Neural-specific inactivation of ShcA results in increased embryonic neural progenitor apoptosis and microencephalyKaren N McFarland
Department of Pathology Neuropathology, Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, Virginia 22908, USA
J Neurosci 26:7885-97. 2006..Together, these data suggest a critical role for ShcA in neural progenitor survival signaling and in regulating brain size... - Differential localization of MAPK-activated protein kinases RSK1 and MSK1 in mouse brainDaniel Heffron
Department of Pathology, University of Virginia Health System, P O Box 800904, Charlottesville, VA 22908, USA
Brain Res Mol Brain Res 136:134-41. 2005....
Research Grants
- MAP KINASE SIGNALING IN ASTROGLIAL REACTIONSJames Mandell; Fiscal Year: 2003..3) To generate transgenic mice expressing astrocyte-targeted activators of the ERK/MAPK pathway as critical tests of the working hypothesis. ..
- Functions of ERK and p38 MAP Kinases in AstrogliosisJames Mandell; Fiscal Year: 2007..This approach could promote recovery of brain and spinal cord function after injury or stroke. ..
- Hypoglycemic signaling targets in astrocytesJames Mandell; Fiscal Year: 2003..The longterm goal is to develop pharmacologic or molecular methods to augment or modulate neuroprotective aspects of the astrocytic response to hypoglycemia. ..