JAMES MALLER

Summary

Affiliation: University of Colorado Health Sciences Center
Country: USA

Publications

  1. pmc Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest
    Brian J Tunquist
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, 4200 E 9th Avenue, Campus Box C236, Denver, CO 80262, USA
    J Cell Biol 163:1231-42. 2003
  2. ncbi request reprint The pathway of MAP kinase mediation of CSF arrest in Xenopus oocytes
    J L Maller
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver 80262, USA
    Biol Cell 93:27-33. 2001
  3. pmc Activation of p90 Rsk1 is sufficient for differentiation of PC12 cells
    Eran Silverman
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, 4200 E 9th Ave, Campus Box C236, Denver, CO 80262, USA
    Mol Cell Biol 24:10573-83. 2004
  4. ncbi request reprint The mechanism of CSF arrest in vertebrate oocytes
    James L Maller
    The Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, 4200 E 9th Avenue, Box C236, Denver, CO 80262, USA
    Mol Cell Endocrinol 187:173-8. 2002
  5. ncbi request reprint Signal transduction. Fishing at the cell surface
    James L Maller
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    Science 300:594-5. 2003
  6. ncbi request reprint Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs
    Brian J Tunquist
    The Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    Genes Dev 17:683-710. 2003
  7. ncbi request reprint The polo box is required for multiple functions of Plx1 in mitosis
    Junjun Liu
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    J Biol Chem 279:21367-73. 2004
  8. ncbi request reprint Regulation of the G(2)/M transition in Xenopus oocytes by the cAMP-dependent protein kinase
    Patrick A Eyers
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    J Biol Chem 280:24339-46. 2005
  9. ncbi request reprint Calcium elevation at fertilization coordinates phosphorylation of XErp1/Emi2 by Plx1 and CaMK II to release metaphase arrest by cytostatic factor
    Junjun Liu
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    Curr Biol 15:1458-68. 2005
  10. ncbi request reprint A feedback loop in the polo-like kinase activation pathway
    Eleanor Erikson
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, 80262, USA
    J Biol Chem 279:32219-24. 2004

Research Grants

  1. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2001
  2. S6 PHOSPHORYLATION AND TYROSINE PROTEIN KINASES
    JAMES MALLER; Fiscal Year: 1999
  3. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2000
  4. S6 PHOSPHORYLATION AND TYROSINE PROTEIN KINASES
    JAMES MALLER; Fiscal Year: 2000
  5. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 1993
  6. S6 Phosphorylation & Tyrosine Protein Kinases
    JAMES MALLER; Fiscal Year: 2002
  7. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2002
  8. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2003
  9. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 1991
  10. S6 Phosphorylation & Tyrosine Protein Kinases
    JAMES MALLER; Fiscal Year: 2003

Collaborators

  • P Thomas
  • William P Schiemann
  • Natalie Ahn
  • M Frodin
  • Andrea L Lewellyn
  • Patrick A Eyers
  • Junjun Liu
  • Frank Eckerdt
  • Aimin Peng
  • Gaetan Pascreau
  • Brian J Tunquist
  • Lin G Chen
  • Bryn Grimison
  • Eleanor Erikson
  • Claude Prigent
  • Tomomi M Yamamoto
  • Mair E A Churchill
  • Liat Josefsberg Ben-Yehoshua
  • Christopher W Conn
  • Eran Silverman
  • Claire E Haydon
  • Carla V Finkielstein
  • Yutaka Matsumoto
  • Meridee Phistry
  • Anna A Depaoli-Roach
  • Nobuhiro R Hayashi
  • Jean Gautier
  • Steen Gammeltoft
  • Timothy A J Haystead
  • Yue Wei Qian
  • Katheryn A Resing
  • Lauren D Aveline-Wolf
  • Markus S Schwab

Detail Information

Publications32

  1. pmc Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest
    Brian J Tunquist
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, 4200 E 9th Avenue, Campus Box C236, Denver, CO 80262, USA
    J Cell Biol 163:1231-42. 2003
    ..These results suggest a model in which CSF arrest by Mos is mediated by the Mad1 and Mad2 proteins in a manner distinct from the spindle checkpoint...
  2. ncbi request reprint The pathway of MAP kinase mediation of CSF arrest in Xenopus oocytes
    J L Maller
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver 80262, USA
    Biol Cell 93:27-33. 2001
    ..CSF arrest in vertebrate oocytes by p90Rsk provides a potential link between the MAPK pathway and the spindle assembly checkpoint in the cell cycle...
  3. pmc Activation of p90 Rsk1 is sufficient for differentiation of PC12 cells
    Eran Silverman
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, 4200 E 9th Ave, Campus Box C236, Denver, CO 80262, USA
    Mol Cell Biol 24:10573-83. 2004
    ..Collectively, our data demonstrate a key role for Rsk1 in the differentiation process of PC12 cells...
  4. ncbi request reprint The mechanism of CSF arrest in vertebrate oocytes
    James L Maller
    The Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, 4200 E 9th Avenue, Box C236, Denver, CO 80262, USA
    Mol Cell Endocrinol 187:173-8. 2002
    ..CSF arrest in vertebrate oocytes by p90(Rsk) provides a link between the MAPK pathway and the spindle assembly checkpoint in the cell cycle...
  5. ncbi request reprint Signal transduction. Fishing at the cell surface
    James L Maller
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    Science 300:594-5. 2003
  6. ncbi request reprint Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs
    Brian J Tunquist
    The Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    Genes Dev 17:683-710. 2003
  7. ncbi request reprint The polo box is required for multiple functions of Plx1 in mitosis
    Junjun Liu
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    J Biol Chem 279:21367-73. 2004
    ..Taken together, these results indicate that the polo box is required for Plx1 function in both the G(2)-M and the metaphase/anaphase transitions during the cell cycle...
  8. ncbi request reprint Regulation of the G(2)/M transition in Xenopus oocytes by the cAMP-dependent protein kinase
    Patrick A Eyers
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    J Biol Chem 280:24339-46. 2005
    ..The identification of substrates for PKAc that maintain cell cycle arrest in G(2) remains an important goal for future work...
  9. ncbi request reprint Calcium elevation at fertilization coordinates phosphorylation of XErp1/Emi2 by Plx1 and CaMK II to release metaphase arrest by cytostatic factor
    Junjun Liu
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    Curr Biol 15:1458-68. 2005
    ..However, connections between the Plx1 pathway and the CaMKII pathway have not been identified...
  10. ncbi request reprint A feedback loop in the polo-like kinase activation pathway
    Eleanor Erikson
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, 80262, USA
    J Biol Chem 279:32219-24. 2004
    ..These results indicate that xPlkk1 may function downstream as a target of Plx1 rather than as an upstream activating kinase during the G(2)/M transition...
  11. ncbi request reprint Metaphase arrest by cyclin E-Cdk2 requires the spindle-checkpoint kinase Mps1
    Bryn Grimison
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    Curr Biol 16:1968-73. 2006
    ..These results uniquely place xMps1 downstream of cyclin E-Cdk2 in mediating a pathway of APC/C inhibition and metaphase arrest...
  12. ncbi request reprint The spindle checkpoint kinase bub1 and cyclin e/cdk2 both contribute to the establishment of meiotic metaphase arrest by cytostatic factor
    Brian J Tunquist
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver 80262, USA
    Curr Biol 12:1027-33. 2002
    ..Both pathways are independent of each other, but each appears to block activation of the APC, which is required for cyclin B degradation and the metaphase/anaphase transition...
  13. ncbi request reprint The anaphase-promoting complex/cyclosome inhibitor Emi2 is essential for meiotic but not mitotic cell cycles
    Junjun Liu
    Howard Hughes Medical Institute HHMI and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    J Biol Chem 281:34736-41. 2006
    ..However, depletion of the protein from cycling egg extracts does not prevent mitotic cell cycle progression. Thus, Emi2 plays an essential role in meiotic but not mitotic cell cycles...
  14. ncbi request reprint The role of Xenopus membrane progesterone receptor beta in mediating the effect of progesterone on oocyte maturation
    Liat Josefsberg Ben-Yehoshua
    Howard Hughes Medical Institute, Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA
    Mol Endocrinol 21:664-73. 2007
    ..These results suggest that XmPRbeta is a physiological progesterone receptor involved in initiating the resumption of meiosis during maturation of Xenopus oocytes...
  15. pmc Undamaged DNA transmits and enhances DNA damage checkpoint signals in early embryos
    Aimin Peng
    Howard Hughes Medical Institute, Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Mol Cell Biol 27:6852-62. 2007
    ....
  16. pmc Discovery of a distinct domain in cyclin A sufficient for centrosomal localization independently of Cdk binding
    Gaetan Pascreau
    Howard Hughes Medical Institute, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 107:2932-7. 2010
    ..These results indicate that the cyclin A CLS may contribute to targeting and recognition of centrosomal Cdk substrates and is required for specific effects of p27(KIP1) on cyclin A-Cdk2...
  17. pmc Regulation of the Aurora B chromosome passenger protein complex during oocyte maturation in Xenopus laevis
    Tomomi M Yamamoto
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Mol Cell Biol 28:4196-203. 2008
    ..Prevention of the decrease in Aurora B activity at fertilization by expression of ectopic wild-type INCENP, but not kinase-dead Aurora B INCENP, blocked calcium-induced exit from metaphase arrest in egg extracts...
  18. pmc Spindle pole regulation by a discrete Eg5-interacting domain in TPX2
    Frank Eckerdt
    Howard Hughes Medical Institute, Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA
    Curr Biol 18:519-25. 2008
    ..Importantly, injection of Eg5 into TPX2-CT-arrested blastomeres causes resumption of cleavage. These results define a discrete domain within the C terminus of TPX2 that exerts a novel Eg5-dependent function in spindle pole segregation...
  19. ncbi request reprint Calcium, calmodulin, and CaMKII requirement for initiation of centrosome duplication in Xenopus egg extracts
    Yutaka Matsumoto
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    Science 295:499-502. 2002
    ..These results indicate that calcium, calmodulin, and CaMKII are required for an essential step in initiation of centrosome duplication. Our data suggest that calcium oscillations in the cell cycle may be linked to centrosome duplication...
  20. pmc Phosphorylation of p53 is regulated by TPX2-Aurora A in xenopus oocytes
    Gaetan Pascreau
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA
    J Biol Chem 284:5497-505. 2009
    ..Our studies suggest that targeting of TPX2 might be an effective strategy for specifically inhibiting the phosphorylation of Aurora A substrates, including p53...
  21. ncbi request reprint Regulation of Xenopus Aurora A activation by TPX2
    Patrick A Eyers
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    J Biol Chem 279:9008-15. 2004
    ..Interestingly, inhibition is blocked by TPX2, suggesting that the ability of Aurora A to transform cells could be regulated by p53, TPX2, or other binding proteins...
  22. ncbi request reprint A role for G1/S cyclin-dependent protein kinases in the apoptotic response to ionizing radiation
    Carla V Finkielstein
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    J Biol Chem 277:38476-85. 2002
    ..These data suggest that caspase cleavage of both cyclin D1-Cdk4 and cyclin A2-Cdk2 promotes specific apoptotic events in embryos undergoing apoptosis in response to ionizing radiation...
  23. ncbi request reprint Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A
    Frank Eckerdt
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Cell Cycle 8:2413-9. 2009
    ..Taken together, our data indicate that Plx1 promotes activation of Aurora A, most likely through TPX2. In light of the current literature, we propose a model in which Plx1 and Aurora A activate each other in a positive feedback loop...
  24. ncbi request reprint Regulating the regulators: Aurora A activation and mitosis
    Patrick A Eyers
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine Denver, Colorado 80262, USA
    Cell Cycle 2:287-9. 2003
  25. doi request reprint Kicking off the polo game
    Frank Eckerdt
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Trends Biochem Sci 33:511-3. 2008
    ..During mitosis, Plk1-dependent Bora degradation promotes Aurora A localization to the centrosome and/or spindle. Bora-dependent regulation provides important new insights into interactions between key mitotic kinases...
  26. ncbi request reprint DNA damage signaling in early Xenopus embryos
    Aimin Peng
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA
    Cell Cycle 7:3-6. 2008
    ..We propose that this novel mode of "in trans" regulation is employed in the DNA damage response to modulate a broad range of chromatin-associated activities in a genome-wide manner...
  27. ncbi request reprint The Aurora A and Aurora B protein kinases: a single amino acid difference controls intrinsic activity and activation by TPX2
    Patrick A Eyers
    Howard Hughes Medical Institute, Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    Cell Cycle 4:784-9. 2005
    ..The generation of an activated mutant of Aurora B will be important for studying its role in cell cycle control and tumorigenesis...
  28. ncbi request reprint Xenopus Polo-like kinase Plx1: a multifunctional mitotic kinase
    Junjun Liu
    Howard Hughes Medical Institute, Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    Oncogene 24:238-47. 2005
    ..Plx1 is also required for cytokinesis and is localized on the midbody of the contractile ring. All known functions of Plx1 require not only its kinase activity but also an intact polo box domain in the C-terminus...
  29. ncbi request reprint A novel mechanism for activation of the protein kinase Aurora A
    Patrick A Eyers
    Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA
    Curr Biol 13:691-7. 2003
    ....
  30. pmc Repo-man controls a protein phosphatase 1-dependent threshold for DNA damage checkpoint activation
    Aimin Peng
    Howard Hughes Medical Institute, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Curr Biol 20:387-96. 2010
    ..Checkpoint activation induced by DNA double-strand breaks (DSB) is dependent on the ATM kinase, a master regulator of the DNA damage response (DDR) that is activated through autophosphorylation and monomerization...
  31. ncbi request reprint The DNA damage checkpoint in embryonic cell cycles is dependent on the DNA-to-cytoplasmic ratio
    Christopher W Conn
    Department of Pharmacology, University of Colorado School of Medicine, Denver 80262 USA
    Dev Cell 7:275-81. 2004
    ....
  32. ncbi request reprint Identification of novel phosphorylation sites on Xenopus laevis Aurora A and analysis of phosphopeptide enrichment by immobilized metal-affinity chromatography
    Claire E Haydon
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
    Mol Cell Proteomics 2:1055-67. 2003
    ..Moreover, we demonstrate a significant difference in enrichment of phosphopeptides when different resins are used for Fe3+-IMAC and characterize the strengths and limitations of this methodology for the study of phosphoproteomics...

Research Grants43

  1. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2001
    ..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
  2. S6 PHOSPHORYLATION AND TYROSINE PROTEIN KINASES
    JAMES MALLER; Fiscal Year: 1999
    ..Aim 2 proposes to investigate the mechanism of activation of Rsk by multi-site phosphorylation mediated by multiple kinases. Aim 3 proposes to determine the mechanisms of inhibition of MAP kinase activation by cAMP. ..
  3. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2000
    ..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
  4. S6 PHOSPHORYLATION AND TYROSINE PROTEIN KINASES
    JAMES MALLER; Fiscal Year: 2000
    ..Aim 2 proposes to investigate the mechanism of activation of Rsk by multi-site phosphorylation mediated by multiple kinases. Aim 3 proposes to determine the mechanisms of inhibition of MAP kinase activation by cAMP. ..
  5. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 1993
    ..The network between MPF and proto-oncogenes like c-src provides a novel basis for investigating the interface between cell cycle control and cell growth control...
  6. S6 Phosphorylation & Tyrosine Protein Kinases
    JAMES MALLER; Fiscal Year: 2002
    ..Experiments will evaluate whether Bub1 mediates CSF arrest by the MAPK pathway. This work provides a link between the MAPK/Rsk pathway and checkpoint control of the cell cycle at the metaphase/anaphase transition. ..
  7. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2002
    ..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
  8. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2003
    ..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
  9. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 1991
    ..The network between MPF and proto-oncogenes like c-src provides a novel basis for investigating the interface between cell cycle control and cell growth control...
  10. S6 Phosphorylation & Tyrosine Protein Kinases
    JAMES MALLER; Fiscal Year: 2003
    ..Experiments will evaluate whether Bub1 mediates CSF arrest by the MAPK pathway. This work provides a link between the MAPK/Rsk pathway and checkpoint control of the cell cycle at the metaphase/anaphase transition. ..
  11. S6 Phosphorylation & Tyrosine Protein Kinases
    JAMES MALLER; Fiscal Year: 2004
    ..Experiments will evaluate whether Bub1 mediates CSF arrest by the MAPK pathway. This work provides a link between the MAPK/Rsk pathway and checkpoint control of the cell cycle at the metaphase/anaphase transition. ..
  12. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 1990
    ..The network between MPF and proto-oncogenes like c-src provides a novel basis for investigating the interface between cell cycle control and cell growth control...
  13. S6 PHOSPHORYLATION AND TYROSINE PROTEIN KINASES
    JAMES MALLER; Fiscal Year: 1993
    ....
  14. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 1992
    ..The network between MPF and proto-oncogenes like c-src provides a novel basis for investigating the interface between cell cycle control and cell growth control...
  15. S6 PHOSPHORYLATION AND TYROSINE PROTEIN KINASES
    JAMES MALLER; Fiscal Year: 1991
    ..By examining both S6 kinases and phosphatases, the complete regulation of this pathway of signal transduction will be evaluated...
  16. S6 PHOSPHORYLATION AND TYROSINE PROTEIN KINASES
    JAMES MALLER; Fiscal Year: 1992
    ..By examining both S6 kinases and phosphatases, the complete regulation of this pathway of signal transduction will be evaluated...
  17. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 1999
    ..The work proposed in this grant should help elucidate the molecular control of the cell cycle at the G2/M transition in normal cells, providing a framework for comparison with the controls in cancer cells. ..
  18. CONTROL OF CELL DIVISION BY CYCLIC AMP
    JAMES MALLER; Fiscal Year: 1980
    ..Such altered phosphoproteins may be candidates for regulatory molecules that maintain the physiological arrest of the oocyte on prophase...
  19. S6 PHOSPHORYLATION AND TYROSINE PROTEIN KINASES
    JAMES MALLER; Fiscal Year: 1990
    ..By examining both S6 kinases and phosphatases, the complete regulation of this pathway of signal transduction will be evaluated...