I G Macara

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. ncbi request reprint Nuclear transport: randy couples
    I G Macara
    Department of Pharmacology, Center for Cell Signaling, University of Virginia, 7196 Hospital West, 577, Health Sciences Center, Charlottesville, Virginia 22908, USA
    Curr Biol 9:R436-9. 1999
  2. ncbi request reprint Closing the GAP between polarity and vesicle transport
    Ian G Macara
    Center for Cell Signaling, Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Cell 125:419-21. 2006
  3. pmc Transport into and out of the nucleus
    I G Macara
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908 0577, USA
    Microbiol Mol Biol Rev 65:570-94, table of contents. 2001
  4. pmc Nucleocytoplasmic shuttling of JAZ, a new cargo protein for exportin-5
    Ting Chen
    Center for Cell Signaling, Department of Microbiology, Health Sciences Center, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    Mol Cell Biol 24:6608-19. 2004
  5. pmc Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export
    M E Lindsay
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22098, USA
    J Cell Biol 153:1391-402. 2001
  6. pmc Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7
    Brandon E Kremer
    Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    Cell 130:837-50. 2007
  7. ncbi request reprint Borg proteins control septin organization and are negatively regulated by Cdc42
    G Joberty
    Markey Center for Cell Signaling and Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 3:861-6. 2001
  8. ncbi request reprint PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
    C L Neudauer
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia, 22908 0577, USA
    Biochem Biophys Res Commun 280:541-7. 2001
  9. ncbi request reprint The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42
    G Joberty
    Markey Center for Cell Signaling, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908 0577, USA
    Nat Cell Biol 2:531-9. 2000
  10. ncbi request reprint Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B
    M E Nemergut
    Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA
    Science 292:1540-3. 2001

Collaborators

Detail Information

Publications78

  1. ncbi request reprint Nuclear transport: randy couples
    I G Macara
    Department of Pharmacology, Center for Cell Signaling, University of Virginia, 7196 Hospital West, 577, Health Sciences Center, Charlottesville, Virginia 22908, USA
    Curr Biol 9:R436-9. 1999
    ....
  2. ncbi request reprint Closing the GAP between polarity and vesicle transport
    Ian G Macara
    Center for Cell Signaling, Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Cell 125:419-21. 2006
    ..2006) provide intriguing evidence for a new pathway that links polarity proteins and vesicle transport to the maintenance of tight junctions, through the control of Cdc42 by Rich1, a GTPase-activating protein...
  3. pmc Transport into and out of the nucleus
    I G Macara
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908 0577, USA
    Microbiol Mol Biol Rev 65:570-94, table of contents. 2001
    ..This review focuses on recent discoveries in the field, with an emphasis on the carriers and cofactors involved in transport and on possible mechanisms for movement through the nuclear pores...
  4. pmc Nucleocytoplasmic shuttling of JAZ, a new cargo protein for exportin-5
    Ting Chen
    Center for Cell Signaling, Department of Microbiology, Health Sciences Center, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    Mol Cell Biol 24:6608-19. 2004
    ..Together, these data suggest that JAZ is exported by exportin-5 but translocates back into nuclei by a facilitated diffusion mechanism...
  5. pmc Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export
    M E Lindsay
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22098, USA
    J Cell Biol 153:1391-402. 2001
    ..We infer that this complex translocates through the nuclear pore to the cytoplasm where it is disassembled by RanBP1 and Ran GTPase--activating protein...
  6. pmc Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7
    Brandon E Kremer
    Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    Cell 130:837-50. 2007
    ..These data illuminate an unanticipated connection between septins, SOCS7, NCK signaling, and the DNA damage response...
  7. ncbi request reprint Borg proteins control septin organization and are negatively regulated by Cdc42
    G Joberty
    Markey Center for Cell Signaling and Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 3:861-6. 2001
    ..Cdc42 negatively regulates this effect and inhibits the binding of Borg3 to septins. Borgs are therefore the first known regulators of mammalian septin organization and provide an unexpected link between the septin and Cdc42 GTPases...
  8. ncbi request reprint PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
    C L Neudauer
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia, 22908 0577, USA
    Biochem Biophys Res Commun 280:541-7. 2001
    ..PIST may function as a scaffolding protein to link TC10 to signaling pathways...
  9. ncbi request reprint The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42
    G Joberty
    Markey Center for Cell Signaling, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908 0577, USA
    Nat Cell Biol 2:531-9. 2000
    ..This assembly is implicated in the formation of normal tight junctions at epithelial cell-cell contacts. Thus, Par6 is a key adaptor that links Cdc42 and atypical PKCs to Par3...
  10. ncbi request reprint Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B
    M E Nemergut
    Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA
    Science 292:1540-3. 2001
    ..We propose that the docking of RCC1 to H2A/H2B establishes the polarity of the Ran-GTP gradient that drives nuclear envelope assembly, nuclear transport, and other nuclear events...
  11. pmc Importin-11, a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2
    S M Plafker
    Center for Cell Signaling, Box 800577, HSC and Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
    EMBO J 19:5502-13. 2000
    ..These data establish importin-11 as a new member of the karyopherin family of transport receptors, and identify UbcM2 as a nuclear member of the E2 ubiquitin-conjugating enzyme family...
  12. ncbi request reprint Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins
    Quansheng Du
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Cell 119:503-16. 2004
    ..We propose that a related switch mechanism might operate in asymmetric cell divisions in the fly and nematode...
  13. ncbi request reprint N-terminal alpha-methylation of RCC1 is necessary for stable chromatin association and normal mitosis
    Ting Chen
    Department of Microbiology, Center for Cell Signaling, University of Virginia School of Medicine University of Virginia, Charlottesville, VA 22908 0577, USA
    Nat Cell Biol 9:596-603. 2007
    ..These data provide the first known function for N-terminal protein methylation...
  14. ncbi request reprint Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import
    Mark E Lindsay
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville 22908, USA
    Cell 110:349-60. 2002
    ..These results identify Npap60 as a cofactor for importin-alpha:beta nuclear import and as a previously unidentified subunit of the importin complex...
  15. ncbi request reprint High affinity Rab3 binding is dispensable for Rabphilin-dependent potentiation of stimulated secretion
    G Joberty
    Markey Center for Cell Signalling, Health Sciences Center, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Sci 112:3579-87. 1999
    ..The effects of Rabphilin on secretion may be mediated through interaction with another, unknown, factor that recognizes the Rab3 binding domain...
  16. pmc STRADalpha regulates LKB1 localization by blocking access to importin-alpha, and by association with Crm1 and exportin-7
    Julia Dorfman
    Program in Biophysics, Department of Microbiology, University of Virginia School of Medicine, Charlottesville VA 22908 0577, USA
    Mol Biol Cell 19:1614-26. 2008
    ..These results identify a multifactored mechanism to control LKB1 localization, and they suggest that the STRADbeta-LKB1 complex might possess unique functions in the nucleus...
  17. pmc Regulation of chromatin binding by a conformational switch in the tail of the Ran exchange factor RCC1
    Yi Hao
    Department of Cell Biology, Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 182:827-36. 2008
    ..We propose that Ran induces an allosteric conformational switch in the tail that exposes the histone-binding surface on RCC1 and facilitates association of the positively charged tail with DNA...
  18. pmc The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin
    Yi Qin
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Cell Biol 171:1061-71. 2005
    ..These results suggest that Scrib stabilizes the coupling between E-cadherin and the catenins and are consistent with the idea that mammalian Scrib could behave as a tumor suppressor by regulating epithelial cell adhesion and migration...
  19. ncbi request reprint The function of the p190 Rho GTPase-activating protein is controlled by its N-terminal GTP binding domain
    N Tatsis
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 273:34631-8. 1998
    ..Taken together these data demonstrate that within the cell, the Rho/Rac GAP activity of p190 can be regulated by the N-terminal GTP binding domain...
  20. ncbi request reprint Random mutagenesis and functional analysis of the Ran-binding protein, RanBP1
    C Petersen
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 275:4081-91. 2000
    ....
  21. ncbi request reprint A mammalian Partner of inscuteable binds NuMA and regulates mitotic spindle organization
    Q Du
    Centre for Cell Signalling and Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 3:1069-75. 2001
    ..Thus, we have identified a mammalian Pins homologue as a key regulator of spindle organization during mitosis...
  22. pmc Nuclear import of the ran exchange factor, RCC1, is mediated by at least two distinct mechanisms
    M E Nemergut
    Department of Microbiology, Markey Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908, USA
    J Cell Biol 149:835-50. 2000
    ..Furthermore, the nuclear import of RCC1 does not require a preexisting Ran gradient or energy. We speculate that this second import pathway evolved to ensure that RCC1 never accumulates in the cytoplasm...
  23. pmc Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1
    K Plafker
    Markey Center for Cell Signaling and Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 20:3510-21. 2000
    ..Shuttling of RanBP1 may function to clear nuclear pores of Ran:GTP, to prevent premature release of import cargo from transport receptors...
  24. ncbi request reprint Assembly of epithelial tight junctions is negatively regulated by Par6
    Lin Gao
    Center for Cell Signaling and Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    Curr Biol 12:221-5. 2002
    ..These results suggest that Cdc42 and Par6 negatively regulate TJ assembly in mammalian epithelial cells...
  25. ncbi request reprint Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor
    Michael E Nemergut
    Department of Microbiology, The Center for Cell Signaling, The University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:17385-8. 2002
    ..By tethering Crm1 to catalytically enhanced RCC1, RanBP3 may lower the entropic barrier for the loading of Ran.GTP onto Crm1. We propose that this provides an additional mechanism by which RanBP3 facilitates export...
  26. pmc RanBP3 contains an unusual nuclear localization signal that is imported preferentially by importin-alpha3
    K Welch
    Markey Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 19:8400-11. 1999
    ..These results demonstrate that members of the importin-alpha family possess distinct preferences for certain NLS sequences and that the NLS consensus sequence is broader than was hitherto suspected...
  27. ncbi request reprint Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex
    Enrico Brugnera
    Beirne Carter Center for Immunology Research and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Nat Cell Biol 4:574-82. 2002
    ..We can also detect a trimeric ELMO1 Dock180 Rac1 complex and ELMO augments the interaction between Dock180 and Rac. We propose that the Dock180 ELMO complex functions as an unconventional two-part exchange factor for Rac...
  28. pmc A systems analysis of importin-{alpha}-{beta} mediated nuclear protein import
    Gregory Riddick
    Center for Cell Signaling, Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Cell Biol 168:1027-38. 2005
    ..The model revealed that inhibition by RCC1 is caused by sequestration of nuclear Ran. Inhibition by Impbeta results from depletion nuclear RanGTP, and, in support of this mechanism, expression of mRFP-Ran reversed the inhibition...
  29. pmc Mammalian septins regulate microtubule stability through interaction with the microtubule-binding protein MAP4
    Brandon E Kremer
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Mol Biol Cell 16:4648-59. 2005
    ..These data identify a novel molecular function for septins in mammalian cells: the modulation of microtubule dynamics through interaction with MAP4...
  30. ncbi request reprint Borg/septin interactions and the assembly of mammalian septin heterodimers, trimers, and filaments
    Peter J Sheffield
    Department of Microbiology and Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 278:3483-8. 2003
    ..Septins associate through their C-terminal coiled-coil domains, and Borg3 appears to recognize the interface between these domains in Sept6 and Sept7...
  31. pmc Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6
    Sarah M Garrard
    Center for Cell Signaling and Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    EMBO J 22:1125-33. 2003
    ..Nuclear magnetic resonance studies indicate that the semi-CRIB motif of Par6 is at least partially structured by the PDZ domain. The structure highlights a novel role for a PDZ domain as a structural scaffold...
  32. ncbi request reprint Isoforms of the polarity protein par6 have distinct functions
    Lin Gao
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908 0577, USA
    J Biol Chem 279:41557-62. 2004
    ..Unexpectedly, the localization of Par6A to cell-cell contacts is Cdc42-independent...
  33. ncbi request reprint Systems analysis of Ran transport
    Alicia E Smith
    Center for Cell Signaling, Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
    Science 295:488-91. 2002
    ..Moreover, it provides the first estimate of the total in vivo flux (520 molecules per NPC per second and predicts that the transport system is robust...
  34. ncbi request reprint Novel biosensors for the detection of estrogen receptor ligands
    Siddhartha De
    Luna Innovations, Inc, Charlottesville, VA 22903, USA
    J Steroid Biochem Mol Biol 96:235-44. 2005
    ..Moreover, we have demonstrated that this FRET technology can be applied to other nuclear receptors, such as the androgen receptor...
  35. pmc Tuba, a Cdc42 GEF, is required for polarized spindle orientation during epithelial cyst formation
    Yi Qin
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Cell Biol 189:661-9. 2010
    ..Together, our findings implicate Tuba as a key activator of the Cdc42 GTPase during epithelial ductal morphogenesis, which in turn activates apical aPKC to ensure that spindles orient parallel to the lateral plane...
  36. pmc Zonula occludens-1 function in the assembly of tight junctions in Madin-Darby canine kidney epithelial cells
    Elizabeth McNeil
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908 0577
    Mol Biol Cell 17:1922-32. 2006
    ..These data reveal an unexpected function for the SH3 domain of ZO-1 in regulating tight junction assembly in epithelial cells and show that cingulin, occludin, or ZO-2 are not limiting for junction assembly in MDCK monolayers...
  37. ncbi request reprint RNA interference techniques to study epithelial cell adhesion and polarity
    Xinyu Chen
    Center for Cell Signalling, University of Virginia School of Medicine, Charlottesville, USA
    Methods Enzymol 406:362-74. 2006
    ..We discuss the potential challenges and problems associated with the RNAi technique and describe basic protocols for suppressing gene expression using a vector-based short hairpin RNA (shRNA) expression system coupled with nucleofection...
  38. ncbi request reprint Par proteins: partners in polarization
    Ian G Macara
    Department of Microbiology, Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Curr Biol 14:R160-2. 2004
    ..Par-6 recruits Par-3, which is actively removed from other regions by Par-1 and Par-5. Inclusion and exclusion together ensure efficient segregation of the polarity proteins...
  39. pmc RNA localization and polarity: from A(PC) to Z(BP)
    Stavroula Mili
    Department of Microbiology, Center for Cell Signaling, University of Virginia, HSC, Charlottesville, 22908 0577, USA
    Trends Cell Biol 19:156-64. 2009
    ..The description of a new RNA localization pathway involving the tumor-suppressor protein APC raises questions regarding coordination between distinct localization pathways and their effects on protein function and cell polarity...
  40. pmc Inhibition of nuclear import by protein kinase B (Akt) regulates the subcellular distribution and activity of the forkhead transcription factor AFX
    A M Brownawell
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 21:3534-46. 2001
    ....
  41. pmc Axon growth-stimulus package includes local translation
    Ian G Macara
    Ian G Macara, Hidekazu Iioka and Stavroula Mili are in the Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908 0577, USA
    Nat Cell Biol 11:919-21. 2009
    ..The polarity protein Par-3 is locally translated in axons in response to factors such as NGF and netrin-1, and this increased expression is necessary for factor-stimulated axonal outgrowth...
  42. pmc NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein
    Christine E Schaner Tooley
    Department of Microbiology, Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Nature 466:1125-8. 2010
    ..Knockdown of NRMT recapitulates the multi-spindle phenotype seen with methylation-defective RCC1 mutants, demonstrating the importance of alpha-N-methylation for normal bipolar spindle formation and chromosome segregation...
  43. pmc Genome-wide screen reveals APC-associated RNAs enriched in cell protrusions
    Stavroula Mili
    Department of Microbiology, Center for Cell Signaling, University of Virginia, HSC, Charlottesville, Virginia 22908 0577, USA
    Nature 453:115-9. 2008
    ..APC is required for the accumulation of transcripts in protrusions. Our results suggest a new type of RNA anchoring mechanism as well as a new, unanticipated function for APC in localizing RNAs...
  44. pmc Widely conserved signaling pathways in the establishment of cell polarity
    Luke Martin McCaffrey
    Department of Microbiology, Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908 5077, USA
    Cold Spring Harb Perspect Biol 1:a001370. 2009
    ....
  45. pmc Polarity and differential inheritance--universal attributes of life?
    Ian G Macara
    Department of Microbiology, Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    Cell 135:801-12. 2008
    ....
  46. pmc The Par3/aPKC interaction is essential for end bud remodeling and progenitor differentiation during mammary gland morphogenesis
    Luke Martin McCaffrey
    Department of Microbiology, Center for Cell Signaling, University of Virginia School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    Genes Dev 23:1450-60. 2009
    ..These results reveal a new function for Par3 in the regulation of progenitor differentiation and epithelial morphogenesis in vivo and demonstrate for the first time an essential requirement for the Par3-aPKC interaction...
  47. pmc Depletion of E-cadherin disrupts establishment but not maintenance of cell junctions in Madin-Darby canine kidney epithelial cells
    Christopher T Capaldo
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    Mol Biol Cell 18:189-200. 2007
    ....
  48. ncbi request reprint Structural analysis of septin 2, 6, and 7 complexes
    Claudia Low
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 281:30697-706. 2006
    ..Taken together, these observations suggest that polymerized filaments could be comprised of laterally arranged septin core subunits...
  49. ncbi request reprint The polarity protein PAR-3 and TIAM1 cooperate in dendritic spine morphogenesis
    Huaye Zhang
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908 0577, USA
    Nat Cell Biol 8:227-37. 2006
    ..Thus, a balance of PAR-3 and TIAM1 is essential to modulate Rac-GTP levels and to allow spine morphogenesis...
  50. pmc Par-3 mediates the inhibition of LIM kinase 2 to regulate cofilin phosphorylation and tight junction assembly
    Xinyu Chen
    Center for Cell Signaling, Department of Microbiology, Health Sciences Center, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 172:671-8. 2006
    ..Our findings identify LIMK2 as a novel target of Par-3 and uncover a molecular mechanism by which Par-3 could regulate actin dynamics during cell polarization...
  51. pmc The PAR-6 polarity protein regulates dendritic spine morphogenesis through p190 RhoGAP and the Rho GTPase
    Huaye Zhang
    Department of Microbiology and Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Dev Cell 14:216-26. 2008
    ..These results define a role for PAR-6 and aPKC in dendritic spine biogenesis and maintenance, and reveal an unexpected link between the PAR-6/aPKC complex and RhoA activity...
  52. pmc Mammalian septins nomenclature
    Ian G Macara
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Mol Biol Cell 13:4111-3. 2002
    ..The human and mouse septin genes will be named SEPT1-SEPT10 and Sept1-Sept10, respectively. Splice variants will be designated by an underscore followed by a lowercase "v" and a number, e.g., SEPT4_v1...
  53. ncbi request reprint LGN blocks the ability of NuMA to bind and stabilize microtubules. A mechanism for mitotic spindle assembly regulation
    Quansheng Du
    Center for Cell Signaling, Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Curr Biol 12:1928-33. 2002
    ..We therefore propose that a simple steric exclusion model can explain the inhibitory effect of LGN on NuMA-dependent mitotic spindle organization...
  54. pmc Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins
    Amy M Brownawell
    Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 156:53-64. 2002
    ..We propose that exportin-5 is not an RNA export factor but instead participates in the regulated translocation of dsRBD proteins to the cytoplasm where they interact with target mRNAs...
  55. pmc Ribosomal protein L12 uses a distinct nuclear import pathway mediated by importin 11
    Scott M Plafker
    Center for Cell Signaling and Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 22:1266-75. 2002
    ..Taken together, these results indicate that rpL12 uses a distinct nuclear import pathway that may contribute to a mechanism for regulating ribosome synthesis and/or maturation...
  56. ncbi request reprint Multiple splice variants of Par3 and of a novel related gene, Par3L, produce proteins with different binding properties
    Lin Gao
    Center for Cell Signaling and Department of Pharmacology, University of Virginia Health Science Center, P O Box 800577, Charlottesville, VA 22908 0577, USA
    Gene 294:99-107. 2002
    ..Despite these differences, the N-terminal region of Par3L, like that of Par3, can disrupt the formation of tight junctions when ectopically expressed in Madin-Darby canine kidney (MDCK) cells...
  57. ncbi request reprint Parsing the polarity code
    Ian G Macara
    Center for Cell Signaling, Department of Microbiology, University of Virginia, Health Sciences Center, Charlottesville, Virginia 22908 0577, USA
    Nat Rev Mol Cell Biol 5:220-31. 2004
  58. ncbi request reprint Par-3 controls tight junction assembly through the Rac exchange factor Tiam1
    Xinyu Chen
    Center for Cell Signaling, Department of Microbiology, HSC, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 7:262-9. 2005
    ..These results define a critical function for Par-3 in tight junction assembly, and reveal a novel mechanism through which Par-3 engages in the spatial regulation of Rac activity and establishment of epithelial polarity...
  59. ncbi request reprint Activation of the Ras-GRF/CDC25Mm exchange factor by lysophosphatidic acid
    R R Mattingly
    Department of Pharmacology, Wayne State University, Detroit, MI 48201, USA
    Cell Signal 11:603-10. 1999
    ....
  60. pmc The Borgs, a new family of Cdc42 and TC10 GTPase-interacting proteins
    G Joberty
    Markey Center for Cell Signaling and Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 19:6585-97. 1999
    ..We propose that the Borg proteins function as negative regulators of Rho GTPase signaling...
  61. ncbi request reprint The use of permeabilized cell systems to study nuclear transport
    Amy M Brownawell
    Department of Pharmacology, Center for Cell Signaling, University of Virginia, Charlottesville, VA, USA
    Methods Mol Biol 189:209-29. 2002
  62. pmc The adapter importin-alpha provides flexible control of nuclear import at the expense of efficiency
    Greg Riddick
    Department of Biochemistry, University of Virginia, Charlottesville, VA 22908, USA
    Mol Syst Biol 3:118. 2007
    ....
  63. ncbi request reprint Fluorescence resonance energy transfer biosensors that detect Ran conformational changes and a Ran x GDP-importin-beta -RanBP1 complex in vitro and in intact cells
    Kendra Plafker
    Center for Cell Signaling and the Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia 22908 0577, USA
    J Biol Chem 277:30121-7. 2002
    ..Nonetheless, a robust cytoplasmic FRET signal was detectable, which suggests that a significant fraction of cytoplasmic Ran.GDP may exist in a ternary complex with RanBP1 and importins...
  64. ncbi request reprint Why FRET about Ran?
    Ian G Macara
    Center for Cell Signaling, and Department of Pharmacology, Box 800577, HSC, University of Virginia, Charlottesville, VA 22908, USA
    Dev Cell 2:379-80. 2002
    ..A unifying explanation of these functions is that RanGTP produces an organizing field or "atmosphere" around chromatin and acts as a spatial marker. This RanGTP field has now been visualized using fluorescent biosensors...
  65. pmc Codependent functions of RSK2 and the apoptosis-promoting factor TIA-1 in stress granule assembly and cell survival
    T S Karin Eisinger-Mathason
    Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Mol Cell 31:722-36. 2008
    ..Hence, RSK2 is a pivotal factor linking the stress response to survival and proliferation...
  66. ncbi request reprint Nuclear RanGTP is not required for targeting small nucleolar RNAs to the nucleolus
    Aarthi Narayanan
    Department of Biochemistry and Molecular Biology, University of Georgia, Life Sciences Building, Athens, GA 30602, USA
    J Cell Sci 116:177-86. 2003
    ....
  67. pmc The PAR proteins: fundamental players in animal cell polarization
    Bob Goldstein
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Dev Cell 13:609-22. 2007
    ..Although the picture of how the PAR proteins function remains incomplete, cell biology and biochemistry are beginning to explain how PAR proteins polarize cells...
  68. ncbi request reprint mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression
    Nathalie Sans
    Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Building 50, Room 4146, 50 South Drive, Bethesda, MD 20892 8027, USA
    Nat Cell Biol 7:1179-90. 2005
    ..mPins changes the number and morphology of dendritic spines and these effects depend on its Galphai interaction domain, thus implicating G-protein signalling in the regulation of postsynaptic structure and trafficking of GluRs...
  69. pmc Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs
    Rui Yi
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3025, Room 426 CARL Building, Research Drive, Durham, NC 27710, USA
    RNA 11:220-6. 2005
    ..These data have implications for the future clinical utilization of shRNAs and also provide a simple method to enhance RNA interference by shRNAs in culture...
  70. pmc Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import
    Scott M Plafker
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Cell Biol 167:649-59. 2004
    ..Together, these findings reveal that Ub charging can function as a nuclear import trigger, and identify a novel link between E2 regulation and karyopherin-mediated transport...
  71. ncbi request reprint The brain-specific double-stranded RNA-binding protein Staufen2: nucleolar accumulation and isoform-specific exportin-5-dependent export
    Paolo Macchi
    Max Planck Institute for Developmental Biology, D 72076 Tubingen, Germany
    J Biol Chem 279:31440-4. 2004
    ..It is tempting to speculate that Stau2(62) binds RNA in the nucleus and assembles into ribonucleoparticles, which are then exported via the Exportin-5 pathway to their final destination...
  72. ncbi request reprint The kinetochore NUPtials
    P Todd Stukenberg
    Nat Cell Biol 5:945-7. 2003
  73. ncbi request reprint Minihelix-containing RNAs mediate exportin-5-dependent nuclear export of the double-stranded RNA-binding protein ILF3
    Carole Gwizdek
    Institut Jacques Monod, Unite Mixte de Recherche 7592, CNRS, Universit├ęs Paris VI et VII, 2 place Jussieu, Tour 43, Paris 75251 Cedex 05, France
    J Biol Chem 279:884-91. 2004
    ..Exportin-5 thus appears as the first example of a nuclear export receptor that mediates RNA export but also promotes transport of proteinaceous cargo through appropriate and specific RNA adaptors...
  74. ncbi request reprint A novel nuclear export signal in Smad1 is essential for its signaling activity
    Zhan Xiao
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    J Biol Chem 278:34245-52. 2003
    ..Although conserved in other R-Smads such as Smad3, NES2 is not functional in these R-Smads because CRM1 overexpression fails to target them to cytoplasm. Possible reasons for this discrepancy are discussed...
  75. ncbi request reprint Direct interaction of two polarity complexes implicated in epithelial tight junction assembly
    Toby W Hurd
    Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, MI 48109 0650, USA
    Nat Cell Biol 5:137-42. 2003
    ..Our data highlight a previously unrecognized link between protein complexes that are essential for epithelial polarity and formation of tight junctions...
  76. ncbi request reprint Structural insight into filament formation by mammalian septins
    Minhajuddin Sirajuddin
    Abteilung Strukturelle Biologie, Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Nature 449:311-5. 2007
    ..The architecture of septin filaments differs fundamentally from that of other cytoskeletal structures...
  77. ncbi request reprint Exportin-5 mediates nuclear export of minihelix-containing RNAs
    Carole Gwizdek
    Institut Jacques Monod, Unite Mixte de Recherche 7592, CNRS, Universit├ęs Paris VI et VII, 2 place Jussieu, Tour 43, 75251 Paris Cedex 05, France
    J Biol Chem 278:5505-8. 2003
    ....
  78. pmc Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs
    Rui Yi
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Genes Dev 17:3011-6. 2003
    ..Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function...