Research Topics
Genomes and GenesSpecies | Jeffrey J LysiakSummaryAffiliation: University of Virginia Country: USA Publications
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Publications
Essential role of neutrophils in germ cell-specific apoptosis following ischemia/reperfusion injury of the mouse testisJ J Lysiak
Department of Urology, The University of Virginia Health Science Center, Charlottesville, VA 22908, USA
Biol Reprod 65:718-25. 2001..Endothelial cell adhesion molecules, particularly E-selectin, play an important role in mediating this pathology...- Caspase 2 activity contributes to the initial wave of germ cell apoptosis during the first round of spermatogenesisShuqiu Zheng
Department of Urology, University of Virginia Health Science System, Charlottesville, Virginia 22908, USA
Biol Reprod 74:1026-33. 2006.... 
A pathway for phagosome maturation during engulfment of apoptotic cellsJason M Kinchen
Beirne Carter Center for Immunology Research, University of Virginia, Charlottesville, VA 22908, USA
Nat Cell Biol 10:556-66. 2008..Finally, we have also identified a previously undescribed biochemical complex containing Vps34, dynamin and Rab5(GDP), thus providing a mechanism for Rab5 recruitment to the nascent phagosome...
Oxidative stress: a common factor in testicular dysfunctionTerry T Turner
Department of Urology, University of Virginia School of Medicine, PO Box 800422, Charlottesville, VA 22908, USA
J Androl 29:488-98. 2008..Testicular oxidative stress appears to be a common feature in much of what underlies male infertility, which suggests that there may be benefits to developing better antioxidant therapies for relevant cases of hypospermatogenesis...- Fluctuations in rat testicular interstitial oxygen tensions are linked to testicular vasomotion: persistence after repair of torsionJ J Lysiak
Departments of Urology and Cell Biology, University of Virginia Health Science Center, Charlottesville, Virginia 22908, USA
Biol Reprod 63:1383-9. 2000..These results demonstrate for the first time a correlation between testicular vasomotion and interstitial PO(2) cycling, and this correlation persists after the repair of testicular torsion... - Hypoxia-inducible factor-1alpha is constitutively expressed in murine Leydig cells and regulates 3beta-hydroxysteroid dehydrogenase type 1 promoter activityJeffrey J Lysiak
Department of Urology, University of Virginia, Charlottesville, VA 22908, USA
J Androl 30:146-56. 2009..In conclusion, HIF-1alpha is constitutively present in the Leydig cells of the murine testis, where it potentially regulates Hsd3b1 transcription, and thus male reproductive function... - Angiogenesis therapy for the treatment of erectile dysfunctionJeffrey J Lysiak
Department of Urology, University of Virginia, Charlottesville, VA 22932, USA
J Sex Med 7:2554-63. 2010..With this in mind, new therapies must be developed. The treatment with angiogenic growth factors such as vascular endothelial cell growth factor (VEGF) may be one such therapy... - Caspase-9-dependent pathway to murine germ cell apoptosis: mediation by oxidative stress, BAX, and caspase 2Jeffrey J Lysiak
Department of Urology, University of Virginia Health Science System, Charlottesville, VA 22908, USA
Cell Tissue Res 328:411-9. 2007.... - Activation of the nuclear factor kappa B pathway following ischemia-reperfusion of the murine testisJeffrey J Lysiak
Department of Urology Box 800422, University of Virginia Health System, Charlottesville, VA 22908, USA
J Androl 26:129-35. 2005..The specific roles of these genes in the testicular response to IR remains to be determined... - Ischemia-reperfusion of the murine testis stimulates the expression of proinflammatory cytokines and activation of c-jun N-terminal kinase in a pathway to E-selectin expressionJeffrey J Lysiak
Department of Urology, University of Virginia Health Science System, Charlottesville, VA 22908, USA
Biol Reprod 69:202-10. 2003..This provides the first evidence of a cytokine/stress-related kinase signaling pathway to E-selectin expression in vivo... - Peptide and nonpeptide reactive oxygen scavengers provide partial rescue of the testis after torsionJeffrey J Lysiak
Department of Urology, University of Virginia Health Science System, Charlottesville 22908, USA
J Androl 23:400-9. 2002..The mechanism by which ROS modulates neutrophil adhesion to venules is presently under investigation, as are additional therapeutic regimens to block oxidative stress... - Molecular pathway of germ cell apoptosis following ischemia/reperfusion of the rat testisJ J Lysiak
Departments of Urology and Cell Biology, The University of Virginia Health Science Center, Charlottesville, Virginia 22908, USA
Biol Reprod 63:1465-72. 2000..Results suggest that germ cell apoptosis following ischemia/reperfusion of the rat testis is initiated through the mitochondria-associated molecule Bax as well as Fas-FasL interactions... 
The role of tumor necrosis factor-alpha and interleukin-1 in the mammalian testis and their involvement in testicular torsion and autoimmune orchitisJeffrey J Lysiak
Department of Urology, University of Virginia, Charlottesville, VA 22908, USA
Reprod Biol Endocrinol 2:9. 2004..Thus, both proinflammatory cytokines, TNF alpha and IL-1, have significant roles in normal testicular functions as well as in certain testicular pathologies...- Unexpected requirement for ELMO1 in clearance of apoptotic germ cells in vivoMichael R Elliott
Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, Virginia 22908, USA
Nature 467:333-7. 2010..Collectively, these findings uncover a selective requirement for ELMO1 in Sertoli-cell-mediated removal of apoptotic germ cells and make a compelling case for a relationship between engulfment and tissue homeostasis in vivo... - Continued clearance of apoptotic cells critically depends on the phagocyte Ucp2 proteinDaeho Park
Center for Cell Clearance, University of Virginia, Charlottesville, Virginia 22908, USA
Nature 477:220-4. 2011..As Ucp2 is linked to metabolic diseases and atherosclerosis, this newly discovered role for Ucp2 in apoptotic cell clearance has implications for the complex aetiology and pathogenesis of these diseases... - Experimental testicular torsion: reperfusion blood flow and subsequent testicular venous plasma testosterone concentrationsTerry T Turner
Department of Urology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
Urology 65:390-4. 2005.... - Phagocytosis of apoptotic cells is regulated by a UNC-73/TRIO-MIG-2/RhoG signaling module and armadillo repeats of CED-12/ELMOColin D deBakker
Department of Microbiology, Beirne Carter Center for Immunology Research, University of Virginia, Charlottesville, VA 22908, USA
Curr Biol 14:2208-16. 2004..However, the components upstream of ELMO and Dock180 during engulfment remain elusive... - BAI1 is an engulfment receptor for apoptotic cells upstream of the ELMO/Dock180/Rac moduleDaeho Park
Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
Nature 450:430-4. 2007..Thus, BAI1 is a phosphatidylserine recognition receptor that can directly recruit a Rac-GEF complex to mediate the uptake of apoptotic cells... - PH domain of ELMO functions in trans to regulate Rac activation via Dock180Mingjian Lu
Beirne Carter Center for Immunology Research, Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
Nat Struct Mol Biol 11:756-62. 2004..These data reveal a new mode of action of PH domains and a novel, evolutionarily conserved mechanism by which a bipartite GEF can activate Rac... - Dock180 and ELMO1 proteins cooperate to promote evolutionarily conserved Rac-dependent cell migrationCynthia M Grimsley
Beirne Carter Center for Immunology Research and the Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
J Biol Chem 279:6087-97. 2004..This finding suggests that Rac activation by the ELMO.Dock180 complex at discrete intracellular locations mediated by the N-terminal 330 amino acids of ELMO1 rather than generalized Rac activation plays a role in cell migration... - Testicular torsion alters the presence of specific proteins in the mouse testis as well as the phosphorylation status of specific proteinsTerry T Turner
Department of Urology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
J Androl 27:285-93. 2006..Knowing these proteins and the pathways to which they point will aid in the search for new therapies of oxidative stress in the testis... - Phagosome maturation: going through the acid testJason M Kinchen
Beirne B Carter Center for Immunology Research and the Department of Microbiology, University of Virginia, Charlottesville, Virginia 22902, USA
Nat Rev Mol Cell Biol 9:781-95. 2008..This suggests that a machinery that is distinct from receptor-mediated endocytosis is used in phagosome maturation... - Engulfment of apoptotic cells: signals for a good mealKodi S Ravichandran
Carter Immunology Center and the Department of Microbiology, University of Virginia, Charlottesville, Virginia, 22908, USA
Nat Rev Immunol 7:964-74. 2007..This Review focuses on these recent developments and attempts to highlight some of the important questions in this field... - "Recruitment signals" from apoptotic cells: invitation to a quiet mealKodi S Ravichandran
Carter Immunology Center and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
Cell 113:817-20. 2003..New observations suggest that corpse clearance is tightly linked to apoptosis and that dying cells use both recruitment and eat-me signals for phagocyte attraction and recognition... - Find-me and eat-me signals in apoptotic cell clearance: progress and conundrumsKodi S Ravichandran
Center for Cell Clearance, University of Virginia, Charlottesville, VA 22908, USA
J Exp Med 207:1807-17. 2010..This perspective discusses recent exciting progress, gaps in our understanding, and the conflicting issues that arise from the newly acquired knowledge... - Identification of two evolutionarily conserved genes regulating processing of engulfed apoptotic cellsJason M Kinchen
Center for Cell Clearance, University of Virginia, Charlottesville, Virginia 22908, USA
Nature 464:778-82. 2010..Taken together, these data identify SAND-1 (Mon1) and CCZ-1 (Ccz1) as critical and evolutionarily conserved components regulating the processing of ingested apoptotic cell corpses... - Dock180-ELMO cooperation in Rac activationMingjian Lu
Carter Immunology Center, University of Virginia, Charlottesville, USA
Methods Enzymol 406:388-402. 2006..Thus, Dock180 and ELMO function together as a bipartite GEF to optimally activate Rac on upstream stimulation to mediate the engulfment of apoptotic cells and cell migration... - Confocal fluorescence microscopy in a murine model of microdissection testicular sperm extraction to improve sperm retrievalRyan P Smith
Department of Urology, University of Virginia, P O Box 800422, Charlottesville, Virginia 22908 0422, USA
J Urol 187:1918-23. 2012..However, localizing sperm foci can be time-consuming and it is not always successful. Fiberoptic confocal fluorescent microscopy offers the advantage of rapid in vivo detection of fluorescently labeled sperm in the seminiferous tubules... - Fiberoptic imaging of cavernous nerves in vivoLisa B Boyette
Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA
J Urol 178:2694-700. 2007..We developed a nontoxic technique that would allow high resolution, in vivo real-time imaging specifically of the cavernous nerves... - Tadalafil increases Akt and extracellular signal-regulated kinase 1/2 activation, and prevents apoptotic cell death in the penis following denervationJeffrey J Lysiak
Department of Urology, University of Virginia, Charlottesville, Virginia, USA
J Urol 179:779-85. 2008..We also determined whether treatment with the phosphodiesterase-5 inhibitor tadalafil prevents apoptosis as well as the specific mechanisms involved... - Cytokine responses to E. coli-induced epididymitis in the rat: blockade by vasectomyTerry T Turner
Department of Urology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
Urology 77:1507.e9-14. 2011..To determine cytokine responses in rat epididymal tissues after retrograde Escherichia coli inoculation of the cauda epididymidis via the intact and obstructed vas deferens... - The phosphatidylserine receptor TIM-4 does not mediate direct signalingDaeho Park
Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, 22908, USA
Curr Biol 19:346-51. 2009..Thus, the transmembrane region and cytoplasmic tail of TIM-4 are dispensable for apoptotic cell engulfment, and we propose that TIM-4 is a PtdSer tethering receptor without any direct signaling of its own... - A Steric-inhibition model for regulation of nucleotide exchange via the Dock180 family of GEFsMingjian Lu
Beirne Carter Center for Immunology Research, Department of Microbiology, University of Virginia, Charlottesville, VA 22908 USA
Curr Biol 15:371-7. 2005..This steric-inhibition model may be a general mechanism for regulating multiple SH3-domain-containing Dock180 family members and may have implications for a variety of biological processes... - Characterization of a novel interaction between ELMO1 and ERM proteinsCynthia M Grimsley
Beirne Carter Center for Immunology Research, and Department of Microbiology, University of Virginia, Lane Road, Charlottesville, VA 22903, USA
J Biol Chem 281:5928-37. 2006..Together these data suggest that ELMO1-mediated cytoskeletal changes may be coordinated with ERM protein crosslinking activity during dynamic cellular functions... - Journey to the grave: signaling events regulating removal of apoptotic cellsJason M Kinchen
Beirne Carter Center for Immunology Research, University of Virginia, Charlottesville, VA 22908, USA
J Cell Sci 120:2143-9. 2007..The recent molecular delineation of the phagocytic process and the identification of novel signaling proteins involved in engulfment have provided an exciting new platform for future studies into this biologically important process... - Engulfment of apoptotic cells is negatively regulated by Rho-mediated signalingAnnie-Carole Tosello-Trampont
Beirne Carter Center for Immunology Research and the Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
J Biol Chem 278:49911-9. 2003.... - Clearance of apoptotic cells: implications in health and diseaseMichael R Elliott
Center for Cell Clearance and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
J Cell Biol 189:1059-70. 2010..A better understanding of the apoptotic cell clearance process in healthy and diseased states could offer new therapeutic strategies... - Nucleotides released by apoptotic cells act as a find-me signal to promote phagocytic clearanceMichael R Elliott
Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, Virginia 22908, USA
Nature 461:282-6. 2009.... - The DOCK180/Elmo complex couples ARNO-mediated Arf6 activation to the downstream activation of Rac1Lorraine C Santy
Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
Curr Biol 15:1749-54. 2005..Together, these data suggest that ARNO and ARF6 coordinate with the Dock180/Elmo complex to promote Rac activation at the leading edge of migrating cells... - Pannexin 1 channels mediate 'find-me' signal release and membrane permeability during apoptosisFaraaz B Chekeni
Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, Virginia 22908, USA
Nature 467:863-7. 2010..Collectively, these data identify PANX1 as a plasma membrane channel mediating the regulated release of find-me signals and selective plasma membrane permeability during apoptosis, and a new mechanism of PANX1 activation by caspases... - Cues for apoptotic cell engulfment: eat-me, don't eat-me and come-get-me signalsCynthia Grimsley
Carter Immunology Center and the Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
Trends Cell Biol 13:648-56. 2003..Once apoptotic cells are identified, their uptake by phagocytes further depends on the molecular machinery highly conserved from Caenorhabditis elegans to mammals... - Phagocytic signaling: you can touch, but you can't eatJason M Kinchen
Beirne Carter Center for Immunology Research, University of Virginia, Charlottesville, VA 22908, USA
Curr Biol 18:R521-4. 2008.... - ShcA mediates the dominant pathway to extracellular signal-regulated kinase activation during early thymic developmentPaul Trampont
Carter Immunology Center, MR4 4072D, Box 801386, University of Virginia, Charlottesville, VA 22908, USA
Mol Cell Biol 26:9035-44. 2006..This work provides the first in vivo demonstration of the relative requirement of upstream adapters in controlling ERK activation during beta selection and suggests a dominant role for ShcA... - CXCR4 acts as a costimulator during thymic beta-selectionPaul C Trampont
Carter Immunology Center, University of Virginia, Charlottesville, Virginia, USA
Nat Immunol 11:162-70. 2010..We also characterize functionally relevant signaling molecules downstream of CXCR4 and the pre-TCR in thymocytes. Our data designate CXCR4 as a costimulator of the pre-TCR during beta-selection... - A nonredundant role for the adapter protein Shc in thymic T cell developmentLi Zhang
Beirne B Carter Center for Immunology Research and Department of Microbiology University of Virginia, Charlottesville, VA 22908, USA
Nat Immunol 3:749-55. 2002..Thus, both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development... - ELMO1 signaling in apoptotic germ cell clearance and spermatogenesisMichael R Elliott
Department of Microbiology, Center for Cell Clearance, University of Virginia, Charlottesville, Virginia, USA
Ann N Y Acad Sci 1209:30-6. 2010..Here we will discuss the role of apoptotic cell clearance during spermatogenesis, with a particular emphasis on ELMO1/Dock180 signaling... - Pallbearer and friends: lending a hand in apoptotic cell clearanceMichael R Elliott
Carter Immunology Center and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
Trends Cell Biol 18:95-7. 2008..This work provides the first link between the cellular processes of ubiquitylation/proteasomal degradation and the ability to clear apoptotic cells efficiently... - Regulation of Arf6 and ACAP1 signaling by the PTB-domain-containing adaptor protein GULPZhong Ma
Carter Immunology Center, Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
Curr Biol 17:722-7. 2007..Because PTB-domain-containing adaptor proteins influence endocytosis and trafficking of membrane proteins and cell migration [15, 16], our data support a model wherein PTB-domain-containing adaptor proteins regulate Arf family proteins... - G2A deficiency in mice promotes macrophage activation and atherosclerosisDavid T Bolick
Cardiovascular Research Center, University of Charlottesville, Charlottesville, Virginia 22908, USA
Circ Res 104:318-27. 2009..In the absence of G2A, increased macrophage activation and decreased apoptosis is associated with accumulation of macrophages in the aorta and increased atherosclerosis... - Identification of a novel macrophage phenotype that develops in response to atherogenic phospholipids via Nrf2Alexandra Kadl
Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
Circ Res 107:737-46. 2010..In atherosclerosis, oxidative tissue damage accompanies chronic inflammation; however, macrophage phenotypic changes in response to oxidatively modified molecules are not known... - Neural-specific inactivation of ShcA results in increased embryonic neural progenitor apoptosis and microencephalyKaren N McFarland
Department of Pathology Neuropathology, Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, Virginia 22908, USA
J Neurosci 26:7885-97. 2006..Together, these data suggest a critical role for ShcA in neural progenitor survival signaling and in regulating brain size... - Death in the CNS: six-microns-underMichael R Elliott
Carter Immunology Center and the Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
Cell 133:393-5. 2008..In this issue, Kurant et al. (2008) describe a new phagocytic receptor, called six-microns-under (SIMU), that promotes engulfment of apoptotic neurons by glial cells in the developing nervous system of Drosophila... - In vivo detection of apoptotic cells in the testis using fluorescence labeled annexin V in a mouse model of testicular torsionSang-Kuk Yang
Department of Urology, Konkuk University School of Medicine, Chungju Hospital, Korea
J Urol 176:830-5. 2006..It shows important confounding factors that must be considered as this new imaging technique is developed for detecting apoptotic cells in vivo in testes or in any other organ...
Research Grants
- Sertoli Cell Mediated Engulfment of Apoptotic Germ CellsKodi S Ravichandran; Fiscal Year: 2010..abstract_text> ..