JOSEPH LUTKENHAUS

Summary

Affiliation: University of Kansas Medical Center
Country: USA

Publications

  1. pmc Recruitment of MinC, an inhibitor of Z-ring formation, to the membrane in Escherichia coli: role of MinD and MinE
    Zonglin Hu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 185:196-203. 2003
  2. pmc The ParA/MinD family puts things in their place
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Trends Microbiol 20:411-8. 2012
  3. doi request reprint Bacterial cytokinesis: From Z ring to divisome
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas
    Cytoskeleton (Hoboken) 69:778-90. 2012
  4. ncbi request reprint Min oscillation in bacteria
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA
    Adv Exp Med Biol 641:49-61. 2008
  5. doi request reprint Biochemistry. Tinkering with acellular division
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Science 320:755-6. 2008
  6. ncbi request reprint Assembly dynamics of the bacterial MinCDE system and spatial regulation of the Z ring
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Annu Rev Biochem 76:539-62. 2007
  7. ncbi request reprint Bacterial cytokinesis: let the light shine in
    J Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Curr Biol 7:R573-5. 1997
  8. ncbi request reprint MinD and role of the deviant Walker A motif, dimerization and membrane binding in oscillation
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 48:295-303. 2003
  9. ncbi request reprint The regulation of bacterial cell division: a time and place for it
    J Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Curr Opin Microbiol 1:210-5. 1998
  10. ncbi request reprint Dynamic proteins in bacteria
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Curr Opin Microbiol 5:548-52. 2002

Research Grants

  1. REGULATION OF CELL DIVISION
    JOSEPH LUTKENHAUS; Fiscal Year: 2007
  2. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH LUTKENHAUS; Fiscal Year: 2007
  3. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH F LUTKENHAUS; Fiscal Year: 2010

Collaborators

Detail Information

Publications29

  1. pmc Recruitment of MinC, an inhibitor of Z-ring formation, to the membrane in Escherichia coli: role of MinD and MinE
    Zonglin Hu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 185:196-203. 2003
    ..These results suggest that MinE induces a conformational change in MinD bound to the bilayer that results in the release of MinC. Also, it is argued that binding of MinD to the membrane activates MinC...
  2. pmc The ParA/MinD family puts things in their place
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Trends Microbiol 20:411-8. 2012
    ..Also, some bacteria lacking MinD use orphan ParAs to regulate cell division. Positioning of MinD/ParA proteins is either due to self-organization on a surface or reliance on a landmark protein that functions as a molecular beacon...
  3. doi request reprint Bacterial cytokinesis: From Z ring to divisome
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas
    Cytoskeleton (Hoboken) 69:778-90. 2012
    ..Spatial regulation of cytokinesis occurs at the stage of Z ring assembly and regulation of cell size occurs at this stage or during Z ring maturation...
  4. ncbi request reprint Min oscillation in bacteria
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA
    Adv Exp Med Biol 641:49-61. 2008
    ..The simplicity and ease of manipulating the Min system make it a tractable model to obtain a complete understanding of a self-organizing system...
  5. doi request reprint Biochemistry. Tinkering with acellular division
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Science 320:755-6. 2008
  6. ncbi request reprint Assembly dynamics of the bacterial MinCDE system and spatial regulation of the Z ring
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Annu Rev Biochem 76:539-62. 2007
    ..Additional gradients of negative regulators of FtsZ assembly are used by E. coli and other bacteria to achieve spatial control of Z-ring assembly...
  7. ncbi request reprint Bacterial cytokinesis: let the light shine in
    J Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Curr Biol 7:R573-5. 1997
    ....
  8. ncbi request reprint MinD and role of the deviant Walker A motif, dimerization and membrane binding in oscillation
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 48:295-303. 2003
    ..The sequential interaction of MinD with these two proteins, which is dictated by the membrane, is critical to the oscillatory mechanism involved in spatial regulation of division...
  9. ncbi request reprint The regulation of bacterial cell division: a time and place for it
    J Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Curr Opin Microbiol 1:210-5. 1998
    ..The localization of these proteins implies the existence of positional information in the cell, but how this information is established is unknown...
  10. ncbi request reprint Dynamic proteins in bacteria
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Curr Opin Microbiol 5:548-52. 2002
    ..These studies further demonstrate that the eukaryotic cytoskeleton had its origins in bacteria...
  11. ncbi request reprint Organelle division: from coli to chloroplasts
    J Lutkenhaus
    Department of Microbiology, University of Kansas Medical Center, Kansas City 66160, USA
    Curr Biol 8:R619-21. 1998
    ..FtsZ, an ancestral homolog of eukaryotic tubulin, assembles into the cytokinetic Z ring that directs cell division in bacteria. Recent results indicate that FtsZ is also used for division by chloroplasts, though not by mitochondria...
  12. ncbi request reprint A conserved sequence at the C-terminus of MinD is required for binding to the membrane and targeting MinC to the septum
    Zonglin Hu
    Department of Microbiology, University of Kansas Medical Center, KS 66160, USA
    Mol Microbiol 47:345-55. 2003
    ..We suggest a model in which the ATP-dependent dimerization of MinD affects the conformation of the C-terminal region, a potential amphipathic helix, triggering membrane binding...
  13. pmc Dynamic assembly of MinD on phospholipid vesicles regulated by ATP and MinE
    Zonglin Hu
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Proc Natl Acad Sci U S A 99:6761-6. 2002
    ..Stimulation of the MinD ATPase by addition of MinE led to disassembly of the tubes and the release of MinD from the vesicles. It is proposed that this MinE-regulated dynamic assembly of MinD underlies MinD oscillation...
  14. pmc The conserved C-terminal tail of FtsZ is required for the septal localization and division inhibitory activity of MinC(C)/MinD
    Bang Shen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 72:410-24. 2009
    ..This binding displaces FtsA and/or ZipA, and more importantly, positions MinC(N) near the FtsZ polymers making it a more effective inhibitor...
  15. pmc Membrane binding by MinD involves insertion of hydrophobic residues within the C-terminal amphipathic helix into the bilayer
    Huaijin Zhou
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160 7420, USA
    J Bacteriol 185:4326-35. 2003
    ..We conclude that membrane binding by MinD involves penetration of the hydrophobic residues within the C-terminal amphipathic helix into the hydrophobic interior of the bilayer...
  16. ncbi request reprint Z ring as executor of bacterial cell division
    Alex Dajkovic
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Mol Microbiol Biotechnol 11:140-51. 2006
    ..Formation of the Z ring represents a major point of both spatial and temporal regulation of cell division. Here we discuss findings concerning the structure and the formation of the ring as well as its spatial and temporal regulation...
  17. pmc Analysis of MinD mutations reveals residues required for MinE stimulation of the MinD ATPase and residues required for MinC interaction
    Huaijin Zhou
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 187:629-38. 2005
    ..This mutant provides evidence that dimerization of MinD is sufficient for MinD to bind the membrane and recruit its partners...
  18. pmc The switch I and II regions of MinD are required for binding and activating MinC
    Huaijin Zhou
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Bacteriol 186:1546-55. 2004
    ..These results indicate that the switch I and II regions of MinD are required for interaction with MinC but not MinE and that the switch II region has a role in activating MinC...
  19. doi request reprint Examination of the interaction between FtsZ and MinCN in E. coli suggests how MinC disrupts Z rings
    Bang Shen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 75:1285-98. 2010
    ..With these results, an updated model for the action of MinC on FtsZ is proposed: MinC interacts with FtsZ to disrupt two interactions, FtsZ-FtsA/ZipA and FtsZ-FtsZ, both of which are essential for Z ring formation...
  20. pmc MinC mutants deficient in MinD- and DicB-mediated cell division inhibition due to loss of interaction with MinD, DicB, or a septal component
    Huaijin Zhou
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 187:2846-57. 2005
    ..These mutations differentiate the interactions of MinC with its partners and further support the model of MinCD- and MinC-DicB-mediated cell division inhibition...
  21. ncbi request reprint Tethering the Z ring to the membrane through a conserved membrane targeting sequence in FtsA
    Sebastien Pichoff
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 55:1722-34. 2005
    ..It is required to target FtsA to the membrane and subsequently to the Z ring. As FtsA is much more widely conserved in bacteria than ZipA, it is likely that FtsA serves as the principal membrane anchor for the Z ring...
  22. pmc Overview of cell shape: cytoskeletons shape bacterial cells
    Sebastien Pichoff
    University of Kansas Medical Center, Microbiology, Molecular Genetics and Immunology, 39th and Rainbow Blvd, Kansas City, KS 66160, United States
    Curr Opin Microbiol 10:601-5. 2007
    ..Cells with other shapes have additional cytoskeletal elements that either localize synthetic machineries or possibly influence their activity...
  23. pmc Unique and overlapping roles for ZipA and FtsA in septal ring assembly in Escherichia coli
    Sebastien Pichoff
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    EMBO J 21:685-93. 2002
    ..These results demonstrate that ZipA and FtsA are both required for recruitment of additional division proteins to the Z ring, but either one is capable of supporting formation and stabilization of Z rings...
  24. pmc Investigation of regulation of FtsZ assembly by SulA and development of a model for FtsZ polymerization
    Alex Dajkovic
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 190:2513-26. 2008
    ..In vivo stoichiometry of the disruption of Z rings by SulA suggests that FtsZ may undergo two cooperative transitions in forming the Z ring...
  25. ncbi request reprint Unexpected twist to the Z ring
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Dev Cell 2:519-21. 2002
    ..Time lapse photography of an FtsZ-GFP fusion reveals that this switch involves a spiral intermediate and allows identification of the specific sporulation functions involved...
  26. ncbi request reprint Identification of a region of FtsA required for interaction with FtsZ
    Sebastien Pichoff
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 64:1129-38. 2007
    ..The results support our model that FtsA is targeted to the membrane before it interacts with FtsZ and demonstrates that this interaction plays an essential role in E. coli cell division...
  27. pmc Soj (ParA) DNA binding is mediated by conserved arginines and is essential for plasmid segregation
    Christina M Hester
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Proc Natl Acad Sci U S A 104:20326-31. 2007
    ....
  28. ncbi request reprint Another cytoskeleton in the closet
    Joe Lutkenhaus
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Cell 115:648-50. 2003
    ..In this issue of Cell, provide evidence that the third family, comprising the intermediate filaments, also has origins in bacteria and is responsible for producing curved cells...
  29. pmc Differences in MinC/MinD sensitivity between polar and internal Z rings in Escherichia coli
    Bang Shen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 193:367-76. 2011
    ..Taken together, these data indicate that polar Z rings are more susceptible to MinC/MinD than internal Z rings, even when MinE is absent...

Research Grants7

  1. REGULATION OF CELL DIVISION
    JOSEPH LUTKENHAUS; Fiscal Year: 2007
    ..It has great similarity to tubulin but is also quite distinct. As a result it should prove to be a useful, novel target for antimicrobial therapy. ..
  2. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH LUTKENHAUS; Fiscal Year: 2007
    ..Finally, the new program has been based on our track record in which eleven investigators supported by the previous COBRE have secured a total of 16 extramural grants. Eight of the eleven have secured 12 NIH grants. ..
  3. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH F LUTKENHAUS; Fiscal Year: 2010
    ..Finally, the new program has been based on our track record in which eleven investigators supported by the previous COBRE have secured a total of 16 extramural grants. Eight of the eleven have secured 12 NIH grants. ..