Xuelian Luo

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi Mitosis: short-circuiting spindle checkpoint signaling
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Curr Biol 22:R128-30. 2012
  2. ncbi Protein metamorphosis: the two-state behavior of Mad2
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Structure 16:1616-25. 2008
  3. ncbi Snapshots of a hybrid transcription factor in the Hippo pathway
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Protein Cell 1:811-9. 2010
  4. ncbi p31comet blocks Mad2 activation through structural mimicry
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Cell 131:744-55. 2007
  5. ncbi Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Biol 6:e50. 2008
  6. ncbi Phosphorylation of the spindle checkpoint protein Mad2 regulates its conformational transition
    Soonjoung Kim
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 107:19772-7. 2010
  7. ncbi Conformation-specific binding of p31(comet) antagonizes the function of Mad2 in the spindle checkpoint
    Guohong Xia
    Department of Pharmacology, The University of Texas, Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    EMBO J 23:3133-43. 2004
  8. ncbi Structural analysis of human Cdc20 supports multisite degron recognition by APC/C
    Wei Tian
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 109:18419-24. 2012
  9. ncbi Purification and assay of Mad2: a two-state inhibitor of anaphase-promoting complex/cyclosome
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Methods Enzymol 398:246-55. 2005
  10. ncbi The Mad2 spindle checkpoint protein has two distinct natively folded states
    Xuelian Luo
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 11:338-45. 2004

Research Grants

  1. Structure and function of the Mad2 checkpoint protein
    Xuelian Luo; Fiscal Year: 2007
  2. Structure and Function of Spindle Checkpoint Proteins
    Xuelian Luo; Fiscal Year: 2010

Collaborators

Detail Information

Publications14

  1. ncbi Mitosis: short-circuiting spindle checkpoint signaling
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Curr Biol 22:R128-30. 2012
    ..Using clever genetic experiments in the budding yeast, Lau and Murray define the endpoint of checkpoint signaling and provide key mechanistic insights into checkpoint inhibition of APC/C...
  2. ncbi Protein metamorphosis: the two-state behavior of Mad2
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Structure 16:1616-25. 2008
    ..This review summarizes recent structural and biochemical studies on the two-state behavior of Mad2 and discusses the generality and implications of structural malleability of proteins...
  3. ncbi Snapshots of a hybrid transcription factor in the Hippo pathway
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Protein Cell 1:811-9. 2010
    ....
  4. ncbi p31comet blocks Mad2 activation through structural mimicry
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Cell 131:744-55. 2007
    ..p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."..
  5. ncbi Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Biol 6:e50. 2008
    ..Collectively, our results establish the existence of a symmetric Mad2 dimer and provide insights into Mad1-assisted conformational activation of Mad2 in the spindle checkpoint...
  6. ncbi Phosphorylation of the spindle checkpoint protein Mad2 regulates its conformational transition
    Soonjoung Kim
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 107:19772-7. 2010
    ..Our results indicate that Mad2 phosphorylation inhibits its function through differentially regulating its binding to Mad1 and Cdc20 and establish that the conformational change of Mad2 is regulated by posttranslational mechanisms...
  7. ncbi Conformation-specific binding of p31(comet) antagonizes the function of Mad2 in the spindle checkpoint
    Guohong Xia
    Department of Pharmacology, The University of Texas, Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    EMBO J 23:3133-43. 2004
    ..Therefore, our results suggest that p31(comet) counteracts the function of Mad2 and is required for the silencing of the spindle checkpoint...
  8. ncbi Structural analysis of human Cdc20 supports multisite degron recognition by APC/C
    Wei Tian
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 109:18419-24. 2012
    ..We propose that low-cooperativity, multisite target recognition enables APC/C to robustly ubiquitinate diverse substrates and helps to drive cell cycle oscillations...
  9. ncbi Purification and assay of Mad2: a two-state inhibitor of anaphase-promoting complex/cyclosome
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Methods Enzymol 398:246-55. 2005
    ..This article describes methods for the purification of the two Mad2 conformers and for the analysis of their activities in APC/C inhibition in Xenopus egg extracts...
  10. ncbi The Mad2 spindle checkpoint protein has two distinct natively folded states
    Xuelian Luo
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 11:338-45. 2004
    ..Our results suggest that the unusual two-state behavior of Mad2 is critical for spindle checkpoint signaling...
  11. ncbi Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting
    Soonjoung Kim
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 109:6549-54. 2012
    ..Our results indicate that CTD is a part of an extensive kinetochore-binding interface of Mad1, and rationalize graded kinetochore targeting of Mad1 during checkpoint signaling...
  12. ncbi Structural and functional analysis of the YAP-binding domain of human TEAD2
    Wei Tian
    Departmentsof Pharmacology and Biochemistry, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 107:7293-8. 2010
    ..Targeting a surface-exposed pocket of TEAD might be an effective strategy to disrupt the YAP-TEAD interaction and to reduce the oncogenic potential of YAP...
  13. ncbi Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 23:377-87. 2006
    ..This spatially separated, multivalent nucleosome binding mode may apply to other chromatin-modifying enzymes that generally contain multiple nucleosome binding modules...
  14. ncbi The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20
    Xuelian Luo
    Department of Biochemistry, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Mol Cell 9:59-71. 2002
    ..Our data suggest that, upon checkpoint activation, Mad1 recruits Mad2 to unattached kinetochores and may promote binding of Mad2 to Cdc20...

Research Grants4

  1. Structure and function of the Mad2 checkpoint protein
    Xuelian Luo; Fiscal Year: 2007
    Dr. Xuelian Luo received her Ph.D. and postdoctoral training in protein structure determination by nuclear magnetic resonance (NMR) spectroscopy. She is currently an Instructor in Dr. Jose Rizo-Rey's lab at UT Southwestern Medical Center...
  2. Structure and Function of Spindle Checkpoint Proteins
    Xuelian Luo; Fiscal Year: 2010
    ..The proposed research will shed light on the molecular mechanism of the spindle checkpoint and help us understand the root causes of aneuploidy. ..