Gary Luker

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint Special conference of the American Association for Cancer Research on molecular imaging in cancer: linking biology, function, and clinical applications in vivo
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cancer Res 62:2195-8. 2002
  2. pmc Imaging CXCL12-CXCR4 signaling in ovarian cancer therapy
    Emma Salomonnson
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS ONE 8:e51500. 2013
  3. pmc Scavenging of CXCL12 by CXCR7 promotes tumor growth and metastasis of CXCR4-positive breast cancer cells
    K E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Oncogene 31:4750-8. 2012
  4. pmc In vivo imaging of ligand receptor binding with Gaussia luciferase complementation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    Nat Med 18:172-7. 2012
  5. pmc Constitutive and chemokine-dependent internalization and recycling of CXCR7 in breast cancer cells to degrade chemokine ligands
    K E Luker
    Department of Radiology, Center for Molecular Imaging, University of Michigan Medical School, 109 Zina Pitcher Place, A526 BSRB, Ann Arbor, MI 48109 2200, USA
    Oncogene 29:4599-610. 2010
  6. ncbi request reprint Optical imaging: current applications and future directions
    Gary D Luker
    University of Michigan, Ann Arbor, Michigan, USA
    J Nucl Med 49:1-4. 2008
  7. pmc Imaging chemokine receptor dimerization with firefly luciferase complementation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, 109 Zina Pitcher Pl, A526 BSRB, Ann Arbor, MI 48109 2200, USA
    FASEB J 23:823-34. 2009
  8. pmc Imaging ligand-dependent activation of CXCR7
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Neoplasia 11:1022-35. 2009
  9. pmc Bioluminescence imaging of reporter mice for studies of infection and inflammation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48019 2200, USA
    Antiviral Res 86:93-100. 2010
  10. doi request reprint Luciferase protein complementation assays for bioluminescence imaging of cells and mice
    Gary D Luker
    Department of Radiology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Methods Mol Biol 680:29-43. 2011

Research Grants

  1. Imaging Vaccina Viral-Host Pathogenesis
    Gary Luker; Fiscal Year: 2007
  2. Microfluidic Models of Metastatic Cancer
    Gary Luker; Fiscal Year: 2009
  3. Molecular Imaging of Chemokine Receptor Signaling
    Gary D Luker; Fiscal Year: 2010
  4. Microfluidic Models of Metastatic Cancer
    Gary D Luker; Fiscal Year: 2010

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Special conference of the American Association for Cancer Research on molecular imaging in cancer: linking biology, function, and clinical applications in vivo
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cancer Res 62:2195-8. 2002
    ....
  2. pmc Imaging CXCL12-CXCR4 signaling in ovarian cancer therapy
    Emma Salomonnson
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS ONE 8:e51500. 2013
    ..These studies establish a molecular imaging reporter system for analyzing CXCL12-CXCR4 signaling in ovarian cancer, which can be used to investigate biology and therapeutic targeting of this pathway in cell-based assays and living mice...
  3. pmc Scavenging of CXCL12 by CXCR7 promotes tumor growth and metastasis of CXCR4-positive breast cancer cells
    K E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Oncogene 31:4750-8. 2012
    ..These studies establish a new in vivo imaging method to quantify chemokine scavenging by CXCR7 in the tumor microenvironment and identify that CXCR7+ cells promote growth and metastasis of CXCR4+ breast cancer cells...
  4. pmc In vivo imaging of ligand receptor binding with Gaussia luciferase complementation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    Nat Med 18:172-7. 2012
    ..We expect this imaging technology to advance research in areas such as ligand-receptor interactions and the development of new therapeutic agents in cell-based assays and small animals...
  5. pmc Constitutive and chemokine-dependent internalization and recycling of CXCR7 in breast cancer cells to degrade chemokine ligands
    K E Luker
    Department of Radiology, Center for Molecular Imaging, University of Michigan Medical School, 109 Zina Pitcher Place, A526 BSRB, Ann Arbor, MI 48109 2200, USA
    Oncogene 29:4599-610. 2010
    ..CXCR7-dependent uptake of chemokines and receptor trafficking were regulated by beta-arrestin 2. These studies establish mechanisms through which CXCR7 regulates the availability of chemokine ligands in the extracellular space...
  6. ncbi request reprint Optical imaging: current applications and future directions
    Gary D Luker
    University of Michigan, Ann Arbor, Michigan, USA
    J Nucl Med 49:1-4. 2008
    ..In this review, we summarize recent advances in optical techniques for molecular imaging and the potential impact for clinical medicine...
  7. pmc Imaging chemokine receptor dimerization with firefly luciferase complementation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, 109 Zina Pitcher Pl, A526 BSRB, Ann Arbor, MI 48109 2200, USA
    FASEB J 23:823-34. 2009
    ..This technology should be valuable for analyzing function and therapeutic modulation of receptor dimerization in intact cells and living mice...
  8. pmc Imaging ligand-dependent activation of CXCR7
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Neoplasia 11:1022-35. 2009
    ....
  9. pmc Bioluminescence imaging of reporter mice for studies of infection and inflammation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48019 2200, USA
    Antiviral Res 86:93-100. 2010
    ....
  10. doi request reprint Luciferase protein complementation assays for bioluminescence imaging of cells and mice
    Gary D Luker
    Department of Radiology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Methods Mol Biol 680:29-43. 2011
    ..This chapter discusses the design of luciferase PCAs appropriate for animal imaging, including construction of reporters, incorporation of reporters into cells and mice, imaging techniques, and data analysis...
  11. doi request reprint Bioluminescent CXCL12 fusion protein for cellular studies of CXCR4 and CXCR7
    Kathryn Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Biotechniques 47:625-32. 2009
    ..These data validate CXCL12-GL as a bioluminescent probe to investigate molecular functions of CXCR4 and CXCR7 and screen for compounds that modulate ligand-receptor binding...
  12. doi request reprint Extended cavity laser enhanced two-photon flow cytometry
    Eric R Tkaczyk
    University of Michigan, Center for Ultrafast Optical Science, Electrical Engineering and Computer Science Department, Ann Arbor, Michigan 48109 2099, USA
    J Biomed Opt 13:041319. 2008
    ....
  13. pmc Microfluidic endothelium for studying the intravascular adhesion of metastatic breast cancer cells
    Jonathan W Song
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA
    PLoS ONE 4:e5756. 2009
    ..Research on the vascular microenvironment has been hindered by challenges in studying this compartment in metastasis under conditions that reproduce in vivo physiology while allowing facile experimental manipulation...
  14. ncbi request reprint Bioluminescence imaging of vaccinia virus: effects of interferon on viral replication and spread
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109 0648, USA
    Virology 341:284-300. 2005
    ..Collectively, our data define a new function for type I interferon signaling in systemic dissemination of vaccinia and validate these reporter viruses for studies of pathogenesis...
  15. pmc Monitoring serial changes in circulating human breast cancer cells in murine xenograft models
    Jean Pierre Eliane
    Breast Oncology Program, Comprehensive Cancer Center, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109 0942, USA
    Cancer Res 68:5529-32. 2008
    ..These results establish a new method for studying CTC in mouse models of epithelial cancer, providing the foundation for studies of molecular regulation of CTC in cancer and CTC as biomarker for therapeutic efficacy...
  16. ncbi request reprint Applications of bioluminescence imaging to the study of infectious diseases
    Martha Hutchens
    Immunology Program, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Cell Microbiol 9:2315-22. 2007
    ..We expect that both the range of organisms and the molecular events able to be studied by BLI will continue to expand, yielding important insights into mechanisms of pathogenesis...
  17. pmc Applications of bioluminescence imaging to antiviral research and therapy: multiple luciferase enzymes and quantitation
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, 109 Zina Pitcher Place, A526 BSRB, Ann Arbor, MI 48109 2200, United States
    Antiviral Res 78:179-87. 2008
    ....
  18. pmc Host-derived CD8+ dendritic cells are required for induction of optimal graft-versus-tumor responses after experimental allogeneic bone marrow transplantation
    Tomomi Toubai
    Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI 48109, USA
    Blood 121:4231-41. 2013
    ..Thus, strategies that modulate host APC subsets without direct manipulation of donor T cells could augment GVT responses and enhance the efficacy of allo-HCT...
  19. doi request reprint Imaging opportunities in radiation oncology
    James M Balter
    Department of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA
    Int J Radiat Oncol Biol Phys 79:342-7. 2011
    ..g., through the American College of Radiology Imaging Network (ACRIN)), and building ties through collaborative research as well as smaller joint workshops and symposia...
  20. doi request reprint Imaging CXCR4 signaling with firefly luciferase complementation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Anal Chem 80:5565-73. 2008
    ..Overall, this research establishes a new imaging reporter for probing CXCR4 signaling in cancer and other diseases regulated by this chemokine receptor...
  21. ncbi request reprint Type I interferon regulates respiratory virus infected dendritic cell maturation and cytokine production
    Brian D Rudd
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Viral Immunol 20:531-40. 2007
    ....
  22. ncbi request reprint TLR3 increases disease morbidity and mortality from vaccinia infection
    Martha Hutchens
    Graduate Program in Immunology, Department of Radiology, Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor 48109 2200, USA
    J Immunol 180:483-91. 2008
    ..To our knowledge, our findings show for the first time that interactions between TLR3 and vaccinia increase viral replication and contribute to detrimental effects of the host immune response to poxviruses...
  23. ncbi request reprint Transgenic reporter mouse for bioluminescence imaging of herpes simplex virus 1 infection in living mice
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109 0648, USA
    Virology 347:286-95. 2006
    ..Collectively, these data establish a new transgenic reporter mouse for infection with HSV-1, thereby enabling in vivo bioluminescence imaging studies of HSV-1 pathogenesis without constructing new reporter viruses...
  24. ncbi request reprint Functions of CXCL12 and CXCR4 in breast cancer
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, 1150 West Medical Center Dr, 9301 MSRB III, Ann Arbor, MI 48109 0648, USA
    Cancer Lett 238:30-41. 2006
    ..We also describe pre-clinical studies indicating the potential to exploit CXCR4 as a new molecular target for diagnosis and treatment of breast cancer in patients...
  25. doi request reprint Real-time bioluminescence imaging of viral pathogenesis
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Methods Mol Biol 574:125-35. 2009
    ..In this chapter, we detail experimental protocols for bioluminescence imaging of viral infections in living mice...
  26. ncbi request reprint Luciferase real-time bioluminescence imaging for the study of viral pathogenesis
    Gary D Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Methods Mol Biol 292:285-96. 2005
    ..This imaging technique allows repetitive, noninvasive monitoring of HSV-1 in living mice. In this chapter, we describe the protocols that we use for in vivo BLI of HSV-1 infection...
  27. pmc Microfluidic hydrodynamic cellular patterning for systematic formation of co-culture spheroids
    Yu suke Torisawa
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
    Integr Biol (Camb) 1:649-54. 2009
    ..This cellular patterning method opens new possibilities for understanding and manipulating interactions between different cell types in 3D...
  28. pmc Protective effect of Toll-like receptor 4 in pulmonary vaccinia infection
    Martha A Hutchens
    Graduate Program in Immunology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 4:e1000153. 2008
    ..These data establish that TLR4 mediates a protective innate immune response against vaccinia virus, which informs development of new vaccines and therapeutic agents targeted against poxviruses...
  29. ncbi request reprint CXCR4 regulates growth of both primary and metastatic breast cancer
    Matthew C P Smith
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri, USA
    Cancer Res 64:8604-12. 2004
    ..These data indicate that CXCR4 is required to initiate proliferation and/or promote survival of breast cancer cells in vivo and suggest that CXCR4 inhibitors will improve treatment of patients with primary and metastatic breast cancer...
  30. pmc Bioluminescence imaging reveals systemic dissemination of herpes simplex virus type 1 in the absence of interferon receptors
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology Departments of Ophthalmology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 77:11082-93. 2003
    ..These data demonstrate different effects of type I and II IFNs in limiting systemic dissemination of HSV-1 and further validate the use of bioluminescence imaging for studies of viral pathogenesis...
  31. ncbi request reprint Imaging 26S proteasome activity and inhibition in living mice
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nat Med 9:969-73. 2003
    ....
  32. ncbi request reprint Molecular imaging of protein-protein interactions: controlled expression of p53 and large T-antigen fusion proteins in vivo
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology and Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cancer Res 63:1780-8. 2003
    ..These data demonstrate that the imaging two-hybrid system responds in a proportional fashion to increasing amounts of interacting proteins in vivo...
  33. pmc Noninvasive bioluminescence imaging of herpes simplex virus type 1 infection and therapy in living mice
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 76:12149-61. 2002
    ..These data demonstrate that bioluminescence imaging can be used for noninvasive, real-time monitoring of HSV-1 infection and therapy in living mice...
  34. ncbi request reprint Molecular imaging of gene expression and protein function in vivo with PET and SPECT
    Vijay Sharma
    Molecular Imaging Center, Mallinckrodt Institute of Radiology and Department of Molecular Biology and Pharmacology, Washington University Medical School, St Louis, Missouri 63110, USA
    J Magn Reson Imaging 16:336-51. 2002
    ....

Research Grants5

  1. Imaging Vaccina Viral-Host Pathogenesis
    Gary Luker; Fiscal Year: 2007
    ..Collectively, the research is expected to facilitate the development of improved vaccines and treatments for smallpox, thereby enhancing our ability to prevent the use of smallpox for bioterrorism. ..
  2. Microfluidic Models of Metastatic Cancer
    Gary Luker; Fiscal Year: 2009
    ..These models should greatly advance our knowledge of metastatic disease and enable more rapid testing and validation of new cancer therapeutics to treat or prevent metastatic disease. ..
  3. Molecular Imaging of Chemokine Receptor Signaling
    Gary D Luker; Fiscal Year: 2010
    ..Using these imaging technologies also will greatly improve our ability to test and validate new therapeutic agents, which we anticipate will lead to improved treatments for patients with cancer. ..
  4. Microfluidic Models of Metastatic Cancer
    Gary D Luker; Fiscal Year: 2010
    ..These models should greatly advance our knowledge of metastatic disease and enable more rapid testing and validation of new cancer therapeutics to treat or prevent metastatic disease. ..