Gary Luker

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi Special conference of the American Association for Cancer Research on molecular imaging in cancer: linking biology, function, and clinical applications in vivo
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cancer Res 62:2195-8. 2002
  2. ncbi Luciferase protein complementation assays for bioluminescence imaging of cells and mice
    Gary D Luker
    Department of Radiology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Methods Mol Biol 680:29-43. 2011
  3. ncbi Optical imaging: current applications and future directions
    Gary D Luker
    University of Michigan, Ann Arbor, Michigan, USA
    J Nucl Med 49:1-4. 2008
  4. ncbi Bioluminescence imaging of reporter mice for studies of infection and inflammation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48019 2200, USA
    Antiviral Res 86:93-100. 2010
  5. ncbi Imaging ligand-dependent activation of CXCR7
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Neoplasia 11:1022-35. 2009
  6. ncbi Imaging chemokine receptor dimerization with firefly luciferase complementation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, 109 Zina Pitcher Pl, A526 BSRB, Ann Arbor, MI 48109 2200, USA
    FASEB J 23:823-34. 2009
  7. ncbi Bioluminescent CXCL12 fusion protein for cellular studies of CXCR4 and CXCR7
    Kathryn Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Biotechniques 47:625-32. 2009
  8. ncbi Extended cavity laser enhanced two-photon flow cytometry
    Eric R Tkaczyk
    University of Michigan, Center for Ultrafast Optical Science, Electrical Engineering and Computer Science Department, Ann Arbor, Michigan 48109 2099, USA
    J Biomed Opt 13:041319. 2008
  9. ncbi Microfluidic endothelium for studying the intravascular adhesion of metastatic breast cancer cells
    Jonathan W Song
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA
    PLoS ONE 4:e5756. 2009
  10. ncbi Bioluminescence imaging of vaccinia virus: effects of interferon on viral replication and spread
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109-0648, USA
    Virology 341:284-300. 2005

Research Grants

  1. Imaging Vaccina Viral-Host Pathogenesis
    Gary Luker; Fiscal Year: 2007
  2. Microfluidic Models of Metastatic Cancer
    Gary Luker; Fiscal Year: 2009
  3. Molecular Imaging of Chemokine Receptor Signaling
    Gary D Luker; Fiscal Year: 2010
  4. Microfluidic Models of Metastatic Cancer
    Gary D Luker; Fiscal Year: 2010

Collaborators

  • Nicholas Lukacs
  • Shuichi Takayama
  • Jeffrey Curtis
  • N Reed Dunnick
  • Yue Cao
  • Robert Jeraj
  • Gikas Mageras
  • Daniel Hayes
  • Gabriela Dontu
  • J M Rae
  • Max S Wicha
  • Jing Yong Ye
  • Anne F Schott
  • Andrzej Myc
  • David Piwnica-Worms
  • Wei Li
  • Kathryn E Luker
  • Mudit Gupta
  • Martha Hutchens
  • James M Balter
  • Jonathan W Song
  • Yu suke Torisawa
  • Kathryn Luker
  • Martha A Hutchens
  • Gabriel Nunez
  • Eric R Tkaczyk
  • Joanne Sonstein
  • Jean Pierre Eliane
  • Brian D Rudd
  • Tracey Schultz
  • Matthew C P Smith
  • Vijay Sharma
  • Jeffrey Yap
  • Rich Wahl
  • Bruce Haffty
  • Gary Kelloff
  • James Galvin
  • Bhadrassain Vikram
  • Keyvan Farahani
  • Soren Bentzen
  • Jeff Michalski
  • Eliot Siegel
  • Vincent Khoo
  • Lalitha Shankar
  • James Deye
  • Rosemary Wong
  • Helen Stone
  • Steve Hahn
  • Jatinder Palta
  • Laurence Clarke
  • Dan Low
  • Barbara Croft
  • Marcus Alber
  • James Balter
  • Robert Atcher
  • Bruce G Haffty
  • Eliot L Siegel
  • Ed Jackson
  • Annik Van den Abbeele
  • Bradley R Foerster
  • K Sue O'Shea
  • Bobak Mosadegh
  • Stephen P Cavnar
  • Maria Morell
  • Ann C Walker
  • Yi Chung Tung
  • Jessica M Steele
  • Madeline Repollet
  • Theodore B Norris
  • James R Baker
  • Alan H Tkaczyk
  • Jean-Pierre Eliane
  • Gerald V Doyle
  • Steve Katnik
  • Peter Sottile
  • Martha Brown
  • RAY S PEEBLES
  • Joseph Romine
  • David A Leib
  • Andrew Pekosz
  • Erin Jackson
  • Julie L Prior
  • Joel R Garbow

Detail Information

Publications29

  1. ncbi Special conference of the American Association for Cancer Research on molecular imaging in cancer: linking biology, function, and clinical applications in vivo
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cancer Res 62:2195-8. 2002
    ....
  2. ncbi Luciferase protein complementation assays for bioluminescence imaging of cells and mice
    Gary D Luker
    Department of Radiology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Methods Mol Biol 680:29-43. 2011
    ..This chapter discusses the design of luciferase PCAs appropriate for animal imaging, including construction of reporters, incorporation of reporters into cells and mice, imaging techniques, and data analysis...
  3. ncbi Optical imaging: current applications and future directions
    Gary D Luker
    University of Michigan, Ann Arbor, Michigan, USA
    J Nucl Med 49:1-4. 2008
    ..In this review, we summarize recent advances in optical techniques for molecular imaging and the potential impact for clinical medicine...
  4. ncbi Bioluminescence imaging of reporter mice for studies of infection and inflammation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48019 2200, USA
    Antiviral Res 86:93-100. 2010
    ....
  5. ncbi Imaging ligand-dependent activation of CXCR7
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Neoplasia 11:1022-35. 2009
    ....
  6. ncbi Imaging chemokine receptor dimerization with firefly luciferase complementation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, 109 Zina Pitcher Pl, A526 BSRB, Ann Arbor, MI 48109 2200, USA
    FASEB J 23:823-34. 2009
    ..This technology should be valuable for analyzing function and therapeutic modulation of receptor dimerization in intact cells and living mice...
  7. ncbi Bioluminescent CXCL12 fusion protein for cellular studies of CXCR4 and CXCR7
    Kathryn Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Biotechniques 47:625-32. 2009
    ..These data validate CXCL12-GL as a bioluminescent probe to investigate molecular functions of CXCR4 and CXCR7 and screen for compounds that modulate ligand-receptor binding...
  8. ncbi Extended cavity laser enhanced two-photon flow cytometry
    Eric R Tkaczyk
    University of Michigan, Center for Ultrafast Optical Science, Electrical Engineering and Computer Science Department, Ann Arbor, Michigan 48109 2099, USA
    J Biomed Opt 13:041319. 2008
    ....
  9. ncbi Microfluidic endothelium for studying the intravascular adhesion of metastatic breast cancer cells
    Jonathan W Song
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA
    PLoS ONE 4:e5756. 2009
    ..Research on the vascular microenvironment has been hindered by challenges in studying this compartment in metastasis under conditions that reproduce in vivo physiology while allowing facile experimental manipulation...
  10. ncbi Bioluminescence imaging of vaccinia virus: effects of interferon on viral replication and spread
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109-0648, USA
    Virology 341:284-300. 2005
    ..Collectively, our data define a new function for type I interferon signaling in systemic dissemination of vaccinia and validate these reporter viruses for studies of pathogenesis...
  11. ncbi Monitoring serial changes in circulating human breast cancer cells in murine xenograft models
    Jean Pierre Eliane
    Breast Oncology Program, Comprehensive Cancer Center, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109 0942, USA
    Cancer Res 68:5529-32. 2008
    ..These results establish a new method for studying CTC in mouse models of epithelial cancer, providing the foundation for studies of molecular regulation of CTC in cancer and CTC as biomarker for therapeutic efficacy...
  12. ncbi Applications of bioluminescence imaging to the study of infectious diseases
    Martha Hutchens
    Immunology Program, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Cell Microbiol 9:2315-22. 2007
    ..We expect that both the range of organisms and the molecular events able to be studied by BLI will continue to expand, yielding important insights into mechanisms of pathogenesis...
  13. ncbi Applications of bioluminescence imaging to antiviral research and therapy: multiple luciferase enzymes and quantitation
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, 109 Zina Pitcher Place, A526 BSRB, Ann Arbor, MI 48109 2200, United States
    Antiviral Res 78:179-87. 2008
    ....
  14. ncbi Imaging opportunities in radiation oncology
    James M Balter
    Department of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA
    Int J Radiat Oncol Biol Phys 79:342-7. 2011
    ..g., through the American College of Radiology Imaging Network (ACRIN)), and building ties through collaborative research as well as smaller joint workshops and symposia...
  15. ncbi Real-time bioluminescence imaging of viral pathogenesis
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Methods Mol Biol 574:125-35. 2009
    ..In this chapter, we detail experimental protocols for bioluminescence imaging of viral infections in living mice...
  16. ncbi Imaging CXCR4 signaling with firefly luciferase complementation
    Kathryn E Luker
    Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Anal Chem 80:5565-73. 2008
    ..Overall, this research establishes a new imaging reporter for probing CXCR4 signaling in cancer and other diseases regulated by this chemokine receptor...
  17. ncbi Type I interferon regulates respiratory virus infected dendritic cell maturation and cytokine production
    Brian D Rudd
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Viral Immunol 20:531-40. 2007
    ....
  18. ncbi TLR3 increases disease morbidity and mortality from vaccinia infection
    Martha Hutchens
    Graduate Program in Immunology, Department of Radiology, Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor 48109 2200, USA
    J Immunol 180:483-91. 2008
    ..To our knowledge, our findings show for the first time that interactions between TLR3 and vaccinia increase viral replication and contribute to detrimental effects of the host immune response to poxviruses...
  19. ncbi Transgenic reporter mouse for bioluminescence imaging of herpes simplex virus 1 infection in living mice
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109-0648, USA
    Virology 347:286-95. 2006
    ..Collectively, these data establish a new transgenic reporter mouse for infection with HSV-1, thereby enabling in vivo bioluminescence imaging studies of HSV-1 pathogenesis without constructing new reporter viruses...
  20. ncbi Functions of CXCL12 and CXCR4 in breast cancer
    Kathryn E Luker
    Department of Radiology, University of Michigan Medical School, 1150 West Medical Center Dr, 9301 MSRB III, Ann Arbor, MI 48109-0648, USA
    Cancer Lett 238:30-41. 2006
    ..We also describe pre-clinical studies indicating the potential to exploit CXCR4 as a new molecular target for diagnosis and treatment of breast cancer in patients...
  21. ncbi Protective effect of Toll-like receptor 4 in pulmonary vaccinia infection
    Martha A Hutchens
    Graduate Program in Immunology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 4:e1000153. 2008
    ..These data establish that TLR4 mediates a protective innate immune response against vaccinia virus, which informs development of new vaccines and therapeutic agents targeted against poxviruses...
  22. ncbi Luciferase real-time bioluminescence imaging for the study of viral pathogenesis
    Gary D Luker
    Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA
    Methods Mol Biol 292:285-96. 2005
    ..This imaging technique allows repetitive, noninvasive monitoring of HSV-1 in living mice. In this chapter, we describe the protocols that we use for in vivo BLI of HSV-1 infection...
  23. ncbi Microfluidic hydrodynamic cellular patterning for systematic formation of co-culture spheroids
    Yu suke Torisawa
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
    Integr Biol (Camb) 1:649-54. 2009
    ..This cellular patterning method opens new possibilities for understanding and manipulating interactions between different cell types in 3D...
  24. ncbi Molecular imaging of gene expression and protein function in vivo with PET and SPECT
    Vijay Sharma
    Molecular Imaging Center, Mallinckrodt Institute of Radiology and Department of Molecular Biology and Pharmacology, Washington University Medical School, St. Louis, Missouri 63110, USA
    J Magn Reson Imaging 16:336-51. 2002
    ....
  25. ncbi Noninvasive bioluminescence imaging of herpes simplex virus type 1 infection and therapy in living mice
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Virol 76:12149-61. 2002
    ..These data demonstrate that bioluminescence imaging can be used for noninvasive, real-time monitoring of HSV-1 infection and therapy in living mice...
  26. ncbi Molecular imaging of protein-protein interactions: controlled expression of p53 and large T-antigen fusion proteins in vivo
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology and Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Cancer Res 63:1780-8. 2003
    ..These data demonstrate that the imaging two-hybrid system responds in a proportional fashion to increasing amounts of interacting proteins in vivo...
  27. ncbi Imaging 26S proteasome activity and inhibition in living mice
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Nat Med 9:969-73. 2003
    ....
  28. ncbi Bioluminescence imaging reveals systemic dissemination of herpes simplex virus type 1 in the absence of interferon receptors
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology Departments of Ophthalmology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 77:11082-93. 2003
    ..These data demonstrate different effects of type I and II IFNs in limiting systemic dissemination of HSV-1 and further validate the use of bioluminescence imaging for studies of viral pathogenesis...
  29. ncbi CXCR4 regulates growth of both primary and metastatic breast cancer
    Matthew C P Smith
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri, USA
    Cancer Res 64:8604-12. 2004
    ..These data indicate that CXCR4 is required to initiate proliferation and/or promote survival of breast cancer cells in vivo and suggest that CXCR4 inhibitors will improve treatment of patients with primary and metastatic breast cancer...

Research Grants5

  1. Imaging Vaccina Viral-Host Pathogenesis
    Gary Luker; Fiscal Year: 2007
    ..Collectively, the research is expected to facilitate the development of improved vaccines and treatments for smallpox, thereby enhancing our ability to prevent the use of smallpox for bioterrorism. ..
  2. Microfluidic Models of Metastatic Cancer
    Gary Luker; Fiscal Year: 2009
    ..These models should greatly advance our knowledge of metastatic disease and enable more rapid testing and validation of new cancer therapeutics to treat or prevent metastatic disease. ..
  3. Molecular Imaging of Chemokine Receptor Signaling
    Gary D Luker; Fiscal Year: 2010
    ..Using these imaging technologies also will greatly improve our ability to test and validate new therapeutic agents, which we anticipate will lead to improved treatments for patients with cancer. ..
  4. Microfluidic Models of Metastatic Cancer
    Gary D Luker; Fiscal Year: 2010
    ..These models should greatly advance our knowledge of metastatic disease and enable more rapid testing and validation of new cancer therapeutics to treat or prevent metastatic disease. ..