Chi Hao Luan

Summary

Affiliation: University of Alabama at Birmingham
Country: USA

Publications

  1. pmc High-throughput expression of C. elegans proteins
    Chi Hao Luan
    Center for Biophysical Sciences and Engineering, Southeast Collaboratory for Structural Genomics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Genome Res 14:2102-10. 2004
  2. ncbi request reprint Structural genomics for Caenorhabditis elegans: high throughput protein expression analysis
    James B Finley
    Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Protein Expr Purif 34:49-55. 2004
  3. pmc Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins
    Yunjia Chen
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    BMC Biotechnol 7:45. 2007
  4. ncbi request reprint A high throughput platform for eukaryotic genes
    Yunjia Chen
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA
    Methods Mol Biol 426:209-20. 2008
  5. ncbi request reprint Crystal structure of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain
    Songlin Li
    Southeast Collaboratory for Structural Genomics, University of Georgia, Athens 30602, USA
    J Biol Chem 277:48596-601. 2002
  6. ncbi request reprint Improving solubility of Shewanella oneidensis MR-1 and Clostridium thermocellum JW-20 proteins expressed into Esherichia coli
    Irina Kataeva
    Southeast Collaboratory for Structural Genomics, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Proteome Res 4:1942-51. 2005
  7. ncbi request reprint Protein production and crystallization at SECSG -- an overview
    Bi Cheng Wang
    Southeast Collaboratory for Structural Genomics, University of Georgia, Athens, GA 30602, USA
    J Struct Funct Genomics 6:233-43. 2005
  8. ncbi request reprint Identification and characterization of nonsubstrate based inhibitors of the essential dengue and West Nile virus proteases
    Vannakambadi K Ganesh
    Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, CBSE 100, 1530, 3rd Avenue South, Birmingham, AL 35294 4400, USA
    Bioorg Med Chem 13:257-64. 2005
  9. ncbi request reprint Identification of the N-terminal functional domains of Cdk5 by molecular truncation and computer modeling
    Jianwen Zhang
    Department of Psychiatry, University of Alabama at Birmingham, Birmingham, Alabama 35294 0017, USA
    Proteins 48:447-53. 2002
  10. ncbi request reprint Antibacterial nicotinamide adenine dinucleotide synthetase inhibitors: amide- and ether-linked tethered dimers with alpha-amino acid end groups
    Sadanandan E Velu
    Department of Chemistry, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 901 14th Street South, Birmingham, Alabama 35294, USA
    J Med Chem 50:2612-21. 2007

Collaborators

Detail Information

Publications10

  1. pmc High-throughput expression of C. elegans proteins
    Chi Hao Luan
    Center for Biophysical Sciences and Engineering, Southeast Collaboratory for Structural Genomics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Genome Res 14:2102-10. 2004
    ..The pipeline described here is applicable to high-throughput expression of recombinant proteins for other species, both prokaryotic and eukaryotic, provided that ORFeome resources become available...
  2. ncbi request reprint Structural genomics for Caenorhabditis elegans: high throughput protein expression analysis
    James B Finley
    Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Protein Expr Purif 34:49-55. 2004
    ..This system successfully automates nearly all aspects of recombinant protein expression analysis including subcloning, bacterial growth, recombinant protein expression, protein purification, and scoring protein solubility...
  3. pmc Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins
    Yunjia Chen
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    BMC Biotechnol 7:45. 2007
    ..Furthermore, a HTP cloning pipeline incorporated with bioinformatics domain/fragment selection methods will be beneficial to studies of structure and function genomics/proteomics...
  4. ncbi request reprint A high throughput platform for eukaryotic genes
    Yunjia Chen
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA
    Methods Mol Biol 426:209-20. 2008
    ..This system can process up to 384 unique samples in parallel and successfully automates most aspects of gene cloning and protein expression analysis, from PCR to protein solubility profiling...
  5. ncbi request reprint Crystal structure of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain
    Songlin Li
    Southeast Collaboratory for Structural Genomics, University of Georgia, Athens 30602, USA
    J Biol Chem 277:48596-601. 2002
    ..Residues in the groove are highly conserved as measured from the information content of the aligned sequences. The C-terminal tail of another molecule in the crystal is bound in this groove...
  6. ncbi request reprint Improving solubility of Shewanella oneidensis MR-1 and Clostridium thermocellum JW-20 proteins expressed into Esherichia coli
    Irina Kataeva
    Southeast Collaboratory for Structural Genomics, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Proteome Res 4:1942-51. 2005
    ..A suggestion has been made that the Wilkinson-Harrison model is not applicable to C. thermocellum proteins because it did not account for the peculiarities of protein sequences from thermophiles...
  7. ncbi request reprint Protein production and crystallization at SECSG -- an overview
    Bi Cheng Wang
    Southeast Collaboratory for Structural Genomics, University of Georgia, Athens, GA 30602, USA
    J Struct Funct Genomics 6:233-43. 2005
    ..Details of the various pipelines in use by the SECSG for protein production and crystallization, as well as some examples of target rescue are described...
  8. ncbi request reprint Identification and characterization of nonsubstrate based inhibitors of the essential dengue and West Nile virus proteases
    Vannakambadi K Ganesh
    Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, CBSE 100, 1530, 3rd Avenue South, Birmingham, AL 35294 4400, USA
    Bioorg Med Chem 13:257-64. 2005
    ..Sequence similarities among flaviviral proteases suggests that such compounds might also possibly inhibit other flaviviral proteases...
  9. ncbi request reprint Identification of the N-terminal functional domains of Cdk5 by molecular truncation and computer modeling
    Jianwen Zhang
    Department of Psychiatry, University of Alabama at Birmingham, Birmingham, Alabama 35294 0017, USA
    Proteins 48:447-53. 2002
    ....
  10. ncbi request reprint Antibacterial nicotinamide adenine dinucleotide synthetase inhibitors: amide- and ether-linked tethered dimers with alpha-amino acid end groups
    Sadanandan E Velu
    Department of Chemistry, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 901 14th Street South, Birmingham, Alabama 35294, USA
    J Med Chem 50:2612-21. 2007
    ..Studies on nonspecific effects, including detergent properties and promiscuous inhibition, suggested little contribution to observed activities...