Affiliation: University of Wisconsin
Ji C, Zhang Y, Xu P, Xu T, Lou X. Nanoscale Landscape of Phosphoinositides Revealed by Specific Pleckstrin Homology (PH) Domains Using Single-molecule Superresolution Imaging in the Plasma Membrane. J Biol Chem. 2015;290:26978-93 pubmed publisher
..These data reveal the nanoscale landscape of key inositol phospholipids in the native PM and imply a framework for local cellular signaling and lipid-protein interactions at a nanometer scale. ..
Ji C, Lou X. Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes. J Vis Exp. 2016;: pubmed publisher
Lou X, Fan F, Messa M, Raimondi A, Wu Y, Looger L, et al
. Reduced release probability prevents vesicle depletion and transmission failure at dynamin mutant synapses. Proc Natl Acad Sci U S A. 2012;109:E515-23 pubmed publisher
Mahapatra S, Fan F, Lou X. Tissue-specific dynamin-1 deletion at the calyx of Held decreases short-term depression through a mechanism distinct from vesicle resupply. Proc Natl Acad Sci U S A. 2016;113:E3150-8 pubmed publisher
..This role comes into play primarily during brief high-frequency stimulation. ..
Ji C, Fan F, Lou X. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep. 2017;20:1409-1421 pubmed publisher
..Thus, different spatiotemporal PI(4,5)P2 signaling regulates distinct steps of vesicle trafficking, and vesicle docking may be a key target of local PI(4,5)P2 signaling in vivo. ..
Fan F, Funk L, Lou X. Dynamin 1- and 3-Mediated Endocytosis Is Essential for the Development of a Large Central Synapse In Vivo. J Neurosci. 2016;36:6097-115 pubmed publisher
..This study highlights the important role of dynamin-mediated endocytosis in the development of central synapses in the mammalian brain. ..