S Lory

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint Structure-function relationship of type-IV prepilin peptidase of Pseudomonas aeruginosa--a review
    S Lory
    Department of Microbiology, University of Washington, Seattle 98195, USA
    Gene 192:117-21. 1997
  2. ncbi request reprint Secretion of proteins and assembly of bacterial surface organelles: shared pathways of extracellular protein targeting
    S Lory
    Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195, USA
    Curr Opin Microbiol 1:27-35. 1998
  3. ncbi request reprint Identification of active-site cysteines in the conserved domain of PilD, the bifunctional type IV pilin leader peptidase/N-methyltransferase of Pseudomonas aeruginosa
    M S Strom
    Department of Microbiology, School of Medicine, University of Washington, Seattle 98195
    J Biol Chem 268:15788-94. 1993
  4. ncbi request reprint Identification and characterization of PilS, an essential regulator of pilin expression in Pseudomonas aeruginosa
    J M Boyd
    Department of Microbiology, University of Washington, Seattle 98195
    Mol Gen Genet 243:565-74. 1994
  5. ncbi request reprint Amino acid substitutions in PilD, a bifunctional enzyme of Pseudomonas aeruginosa. Effect on leader peptidase and N-methyltransferase activities in vitro and in vivo
    J C Pepe
    Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 273:19120-9. 1998
  6. pmc Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin gene
    K S Ishimoto
    Department of Microbiology, School of Medicine, University of Washington, Seattle 98195
    J Bacteriol 174:3514-21. 1992
  7. ncbi request reprint Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
    C K Stover
    PathoGenesis Corporation, Seattle, Washington 98119, USA
    Nature 406:959-64. 2000
  8. ncbi request reprint Gene expression in Pseudomonas aeruginosa biofilms
    M Whiteley
    Department of Microbiology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    Nature 413:860-4. 2001
  9. pmc Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa
    S Jin
    Department of Microbiology, University of Washington, Seattle 98195
    J Bacteriol 176:1316-22. 1994
  10. pmc Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherence
    D A Simpson
    Department of Microbiology, University of Washington, Seattle 98195, USA
    Infect Immun 63:2950-7. 1995

Collaborators

Detail Information

Publications27

  1. ncbi request reprint Structure-function relationship of type-IV prepilin peptidase of Pseudomonas aeruginosa--a review
    S Lory
    Department of Microbiology, University of Washington, Seattle 98195, USA
    Gene 192:117-21. 1997
    ..This review discusses some of the work begun in order to answer questions regarding the structure-function relationships of the active sites of this unique enzyme...
  2. ncbi request reprint Secretion of proteins and assembly of bacterial surface organelles: shared pathways of extracellular protein targeting
    S Lory
    Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195, USA
    Curr Opin Microbiol 1:27-35. 1998
    ....
  3. ncbi request reprint Identification of active-site cysteines in the conserved domain of PilD, the bifunctional type IV pilin leader peptidase/N-methyltransferase of Pseudomonas aeruginosa
    M S Strom
    Department of Microbiology, School of Medicine, University of Washington, Seattle 98195
    J Biol Chem 268:15788-94. 1993
    ....
  4. ncbi request reprint Identification and characterization of PilS, an essential regulator of pilin expression in Pseudomonas aeruginosa
    J M Boyd
    Department of Microbiology, University of Washington, Seattle 98195
    Mol Gen Genet 243:565-74. 1994
    ....
  5. ncbi request reprint Amino acid substitutions in PilD, a bifunctional enzyme of Pseudomonas aeruginosa. Effect on leader peptidase and N-methyltransferase activities in vitro and in vivo
    J C Pepe
    Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 273:19120-9. 1998
    ..Although complete methylation does not appear to be absolutely required for pilus assembly in P. aeruginosa, this modification may be important for pilus function in its natural habitat...
  6. pmc Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin gene
    K S Ishimoto
    Department of Microbiology, School of Medicine, University of Washington, Seattle 98195
    J Bacteriol 174:3514-21. 1992
    ....
  7. ncbi request reprint Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
    C K Stover
    PathoGenesis Corporation, Seattle, Washington 98119, USA
    Nature 406:959-64. 2000
    ..We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances...
  8. ncbi request reprint Gene expression in Pseudomonas aeruginosa biofilms
    M Whiteley
    Department of Microbiology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    Nature 413:860-4. 2001
    ..Our identification of biofilm-regulated genes points to mechanisms of biofilm resistance to antibiotics...
  9. pmc Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa
    S Jin
    Department of Microbiology, University of Washington, Seattle 98195
    J Bacteriol 176:1316-22. 1994
    ..Since rpoN mutants of P. aeruginosa display this same amino acid requirement for growth, the ORF2 product very likely functions as a coinducer of some but not all of the RpoN-controlled genes...
  10. pmc Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherence
    D A Simpson
    Department of Microbiology, University of Washington, Seattle 98195, USA
    Infect Immun 63:2950-7. 1995
    ..Further study should reveal the precise role of P. aeruginosa FliO in non-pilus-mediated adherence, which could include regulation of expression or localization of a nonpilus adhesin...
  11. pmc Detection of simple mutations and polymorphisms in large genomic regions
    E V Sokurenko
    Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195, USA
    Nucleic Acids Res 29:E111. 2001
    ....
  12. pmc A specific targeting domain in mature exotoxin A is required for its extracellular secretion from Pseudomonas aeruginosa
    H M Lu
    Department of Microbiology, School of Medicine, University of Washington, Seattle, 98195, USA
    EMBO J 15:429-36. 1996
    ..In the three- dimensional view, the targeting region is on the toxin surface where it is easily accessible to the components of the extracellular secretion machinery...
  13. pmc Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa
    X Liang
    Departments of Microbiology, University of Washington Genome Center, University of Washington, Seattle, Washington 98195, USA
    J Bacteriol 183:843-53. 2001
    ..Alternatively, one or both of the transcriptional regulators encoded in PAGI-1 may control the expression of genes in the P. aeruginosa chromosome, which provides a selective advantage for strains that have acquired this genomic island...
  14. pmc Components of the protein-excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidase
    D N Nunn
    Department of Microbiology, School of Medicine, University of Washington, Seattle 98195
    Proc Natl Acad Sci U S A 89:47-51. 1992
    ..Evidence suggests that similar components play a role in protein excretion in a wide variety of Gram-negative bacteria...
  15. ncbi request reprint A second prepilin peptidase gene in Escherichia coli K-12
    O Francetic
    Unité de Génétique Moléculaire, Institut Pasteur, Paris, France
    Mol Microbiol 27:763-75. 1998
    ..Polymerase chain reaction and Southern hybridization analyses revealed the presence of the pppA gene in 12 out of 15 E. coli isolates...
  16. ncbi request reprint Posttranslational processing of type IV prepilin and homologs by PilD of Pseudomonas aeruginosa
    M S Strom
    Utilization Research Division, Northwest Fisheries Science Center, NMFS, NOAA, Seattle, Washington 98112
    Methods Enzymol 235:527-40. 1994
    ..aeruginosa, such inhibitors may be useful tools in the study of the role these peptidases play in bacterial virulence...
  17. pmc A plasmid-encoded prepilin peptidase gene from enteropathogenic Escherichia coli
    H Z Zhang
    Department of Medicine, University of Maryland at Baltimore
    J Bacteriol 176:6885-91. 1994
    ..coli utilizes a specific prepilin peptidase to process the major subunit of the bundle-forming pilus. This prepilin petidase contains sequence and reciprocal functional homologies with the PilD protein of P. aeruginosa...
  18. ncbi request reprint Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genes
    K Johnson
    J Biol Chem 261:15703-8. 1986
    ..Several other Pseudomonas promoters and the promoters of the homologous pilin genes from other bacterial species also share homology to this sequence...
  19. pmc A genomic island in Pseudomonas aeruginosa carries the determinants of flagellin glycosylation
    S K Arora
    Department of Medicine/Infectious Diseases, University of Florida, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 98:9342-7. 2001
    ....
  20. pmc The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation
    I Vallet
    , , Institut de Biologie Structurale et Microbiologie/Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Proc Natl Acad Sci U S A 98:6911-6. 2001
    ..These structures appear to be differentially regulated and may function at distinct stages of biofilm formation, or in specific environments colonized by this organism...
  21. ncbi request reprint Pseudomonas aeruginosa infection of respiratory epithelium in a cystic fibrosis xenograft model
    L A Cohn
    Department of Veterinary Medicine and Surgery, University of Missouri-Columbia, Columbia, Missouri 65212, USA
    J Infect Dis 183:919-27. 2001
    ..This model appears to be suitable for genetic study of P. aeruginosa virulence but not of the CF respiratory tract's unique susceptibility...
  22. pmc Identification of two distinct types of flagellar cap proteins, FliD, in Pseudomonas aeruginosa
    S K Arora
    Department of Medicine Infectious Diseases, University of Florida, Gainesville, Florida 32610, USA
    Infect Immun 68:1474-9. 2000
    ..aeruginosa PAO1 was constructed. The motility defect of this mutant and a previously constructed PAK fliD mutant was better complemented with the fliD gene of the homologous types...
  23. pmc Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection
    J Wang
    Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205, USA
    Proc Natl Acad Sci U S A 93:10434-9. 1996
    ..The successful isolation of genetic loci that affect bacterial virulence demonstrates the utility of the IVET system in identification of new virulence genes of P. aeruginosa...
  24. pmc Cloning and characterization of Pseudomonas aeruginosa fliF, necessary for flagellar assembly and bacterial adherence to mucin
    S K Arora
    Department of Medicine Infectious Diseases, University of Florida, Gainsville 32610, USA
    Infect Immun 64:2130-6. 1996
    ..The characterization of this gene as being responsible for the loss of adhesion indicates that basal body structures are probably important for localization of the adhesin...
  25. pmc Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like protein
    K R Hardie
    Unité de Génétique Moléculaire, CNRS URA 1149, Institut Pasteur, Paris, France
    EMBO J 15:978-88. 1996
    ..PulS is only the second component of the main terminal branch of the GSP for which a precise function can be proposed...
  26. ncbi request reprint The fliA (rpoF) gene of Pseudomonas aeruginosa encodes an alternative sigma factor required for flagellin synthesis
    M N Starnbach
    Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305
    Mol Microbiol 6:459-69. 1992
    ..A reading frame downstream of fliA was found to encode the P. aeruginosa homologue of the enterobacterial cheY gene...
  27. ncbi request reprint The multi-talented bacterial adenylate cyclases
    S Lory
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston 02115, USA
    Int J Med Microbiol 293:479-82. 2004
    ....