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Genomes and GenesSpecies | S LorySummaryAffiliation: University of Washington Country: USA Publications
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Publications
Structure-function relationship of type-IV prepilin peptidase of Pseudomonas aeruginosa--a reviewS Lory
Department of Microbiology, University of Washington, Seattle 98195, USA
Gene 192:117-21. 1997..This review discusses some of the work begun in order to answer questions regarding the structure-function relationships of the active sites of this unique enzyme...- Secretion of proteins and assembly of bacterial surface organelles: shared pathways of extracellular protein targetingS Lory
Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195, USA
Curr Opin Microbiol 1:27-35. 1998.... - Identification of active-site cysteines in the conserved domain of PilD, the bifunctional type IV pilin leader peptidase/N-methyltransferase of Pseudomonas aeruginosaM S Strom
Department of Microbiology, School of Medicine, University of Washington, Seattle 98195
J Biol Chem 268:15788-94. 1993.... - Identification and characterization of PilS, an essential regulator of pilin expression in Pseudomonas aeruginosaJ M Boyd
Department of Microbiology, University of Washington, Seattle 98195
Mol Gen Genet 243:565-74. 1994.... - Amino acid substitutions in PilD, a bifunctional enzyme of Pseudomonas aeruginosa. Effect on leader peptidase and N-methyltransferase activities in vitro and in vivoJ C Pepe
Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 273:19120-9. 1998..Although complete methylation does not appear to be absolutely required for pilus assembly in P. aeruginosa, this modification may be important for pilus function in its natural habitat... 
Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin geneK S Ishimoto
Department of Microbiology, School of Medicine, University of Washington, Seattle 98195
J Bacteriol 174:3514-21. 1992....- Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogenC K Stover
PathoGenesis Corporation, Seattle, Washington 98119, USA
Nature 406:959-64. 2000..We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances... - Gene expression in Pseudomonas aeruginosa biofilmsM Whiteley
Department of Microbiology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Nature 413:860-4. 2001..Our identification of biofilm-regulated genes points to mechanisms of biofilm resistance to antibiotics... - Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosaS Jin
Department of Microbiology, University of Washington, Seattle 98195
J Bacteriol 176:1316-22. 1994..Since rpoN mutants of P. aeruginosa display this same amino acid requirement for growth, the ORF2 product very likely functions as a coinducer of some but not all of the RpoN-controlled genes... - Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherenceD A Simpson
Department of Microbiology, University of Washington, Seattle 98195, USA
Infect Immun 63:2950-7. 1995..Further study should reveal the precise role of P. aeruginosa FliO in non-pilus-mediated adherence, which could include regulation of expression or localization of a nonpilus adhesin... - Detection of simple mutations and polymorphisms in large genomic regionsE V Sokurenko
Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195, USA
Nucleic Acids Res 29:E111. 2001.... - A specific targeting domain in mature exotoxin A is required for its extracellular secretion from Pseudomonas aeruginosaH M Lu
Department of Microbiology, School of Medicine, University of Washington, Seattle, 98195, USA
EMBO J 15:429-36. 1996..In the three- dimensional view, the targeting region is on the toxin surface where it is easily accessible to the components of the extracellular secretion machinery... - Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosaX Liang
Departments of Microbiology, University of Washington Genome Center, University of Washington, Seattle, Washington 98195, USA
J Bacteriol 183:843-53. 2001..Alternatively, one or both of the transcriptional regulators encoded in PAGI-1 may control the expression of genes in the P. aeruginosa chromosome, which provides a selective advantage for strains that have acquired this genomic island... - Components of the protein-excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidaseD N Nunn
Department of Microbiology, School of Medicine, University of Washington, Seattle 98195
Proc Natl Acad Sci U S A 89:47-51. 1992..Evidence suggests that similar components play a role in protein excretion in a wide variety of Gram-negative bacteria... - A second prepilin peptidase gene in Escherichia coli K-12O Francetic
Unité de Génétique Moléculaire, Institut Pasteur, Paris, France
Mol Microbiol 27:763-75. 1998..Polymerase chain reaction and Southern hybridization analyses revealed the presence of the pppA gene in 12 out of 15 E. coli isolates... - Posttranslational processing of type IV prepilin and homologs by PilD of Pseudomonas aeruginosaM S Strom
Utilization Research Division, Northwest Fisheries Science Center, NMFS, NOAA, Seattle, Washington 98112
Methods Enzymol 235:527-40. 1994..aeruginosa, such inhibitors may be useful tools in the study of the role these peptidases play in bacterial virulence... - A plasmid-encoded prepilin peptidase gene from enteropathogenic Escherichia coliH Z Zhang
Department of Medicine, University of Maryland at Baltimore
J Bacteriol 176:6885-91. 1994..coli utilizes a specific prepilin peptidase to process the major subunit of the bundle-forming pilus. This prepilin petidase contains sequence and reciprocal functional homologies with the PilD protein of P. aeruginosa... - Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genesK Johnson
J Biol Chem 261:15703-8. 1986..Several other Pseudomonas promoters and the promoters of the homologous pilin genes from other bacterial species also share homology to this sequence... - A genomic island in Pseudomonas aeruginosa carries the determinants of flagellin glycosylationS K Arora
Department of Medicine/Infectious Diseases, University of Florida, Gainesville, FL 32610, USA
Proc Natl Acad Sci U S A 98:9342-7. 2001.... - The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formationI Vallet
, , Institut de Biologie Structurale et Microbiologie/Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Proc Natl Acad Sci U S A 98:6911-6. 2001..These structures appear to be differentially regulated and may function at distinct stages of biofilm formation, or in specific environments colonized by this organism... - Pseudomonas aeruginosa infection of respiratory epithelium in a cystic fibrosis xenograft modelL A Cohn
Department of Veterinary Medicine and Surgery, University of Missouri-Columbia, Columbia, Missouri 65212, USA
J Infect Dis 183:919-27. 2001..This model appears to be suitable for genetic study of P. aeruginosa virulence but not of the CF respiratory tract's unique susceptibility... - Identification of two distinct types of flagellar cap proteins, FliD, in Pseudomonas aeruginosaS K Arora
Department of Medicine Infectious Diseases, University of Florida, Gainesville, Florida 32610, USA
Infect Immun 68:1474-9. 2000..aeruginosa PAO1 was constructed. The motility defect of this mutant and a previously constructed PAK fliD mutant was better complemented with the fliD gene of the homologous types... - Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selectionJ Wang
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205, USA
Proc Natl Acad Sci U S A 93:10434-9. 1996..The successful isolation of genetic loci that affect bacterial virulence demonstrates the utility of the IVET system in identification of new virulence genes of P. aeruginosa... - Cloning and characterization of Pseudomonas aeruginosa fliF, necessary for flagellar assembly and bacterial adherence to mucinS K Arora
Department of Medicine Infectious Diseases, University of Florida, Gainsville 32610, USA
Infect Immun 64:2130-6. 1996..The characterization of this gene as being responsible for the loss of adhesion indicates that basal body structures are probably important for localization of the adhesin... - Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like proteinK R Hardie
Unité de Génétique Moléculaire, CNRS URA 1149, Institut Pasteur, Paris, France
EMBO J 15:978-88. 1996..PulS is only the second component of the main terminal branch of the GSP for which a precise function can be proposed... - The fliA (rpoF) gene of Pseudomonas aeruginosa encodes an alternative sigma factor required for flagellin synthesisM N Starnbach
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305
Mol Microbiol 6:459-69. 1992..A reading frame downstream of fliA was found to encode the P. aeruginosa homologue of the enterobacterial cheY gene... - The multi-talented bacterial adenylate cyclasesS Lory
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston 02115, USA
Int J Med Microbiol 293:479-82. 2004....