Leslie Loew

Summary

Affiliation: University of Connecticut Health Center
Country: USA

Publications

  1. pmc Kinetic analysis of receptor-activated phosphoinositide turnover
    Chang Xu
    Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Biol 161:779-91. 2003
  2. pmc Computational analysis of Rho GTPase cycling
    Cibele Vieira Falkenberg
    Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut, United States of America
    PLoS Comput Biol 9:e1002831. 2013
  3. pmc Cardiac electrophysiological imaging systems scalable for high-throughput drug testing
    Peter Lee
    Department of Physics, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, UK
    Pflugers Arch 464:645-56. 2012
  4. pmc Computational analysis of calcium signaling and membrane electrophysiology in cerebellar Purkinje neurons associated with ataxia
    Sherry Ann Brown
    Richard D, Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
    BMC Syst Biol 6:70. 2012
  5. pmc Where does all the PIP2 come from?
    Leslie M Loew
    Richard D Berlin Center for Cell Analysis and Modelling, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Physiol 582:945-51. 2007
  6. ncbi request reprint The Virtual Cell: a software environment for computational cell biology
    L M Loew
    Center for Biomedical Imaging Technology, Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Trends Biotechnol 19:401-6. 2001
  7. ncbi request reprint STAT module can function as a biphasic amplitude filter
    V Mayya
    Department of Cell Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington 06030, USA
    Syst Biol (Stevenage) 2:43-52. 2005
  8. pmc Modeling and analysis of calcium signaling events leading to long-term depression in cerebellar Purkinje cells
    Nicholas Hernjak
    Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut
    Biophys J 89:3790-806. 2005
  9. pmc Activation of phospholipase C increases intramembrane electric fields in N1E-115 neuroblastoma cells
    Chang Xu
    Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Biophys J 84:4144-56. 2003
  10. ncbi request reprint Geometry shapes cell signaling network output
    Raquell M Holmes
    University of Connecticut Health Center, Center for Cell Analysis and Modeling, Farmington, CT 06032 1507, USA
    Chem Biol 15:523-4. 2008

Research Grants

Collaborators

Detail Information

Publications31

  1. pmc Kinetic analysis of receptor-activated phosphoinositide turnover
    Chang Xu
    Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Biol 161:779-91. 2003
    ....
  2. pmc Computational analysis of Rho GTPase cycling
    Cibele Vieira Falkenberg
    Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut, United States of America
    PLoS Comput Biol 9:e1002831. 2013
    ..In a similar fashion, the charts can be used as a guide in assessing how targeted modifications may compensate for altered GTPase-GDI balance in disease scenarios...
  3. pmc Cardiac electrophysiological imaging systems scalable for high-throughput drug testing
    Peter Lee
    Department of Physics, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, UK
    Pflugers Arch 464:645-56. 2012
    ..Using our simplified systems, we were able to monitor simultaneously the marked changes in the voltage and calcium transients that are responsible for the negative inotropic effect of the compound...
  4. pmc Computational analysis of calcium signaling and membrane electrophysiology in cerebellar Purkinje neurons associated with ataxia
    Sherry Ann Brown
    Richard D, Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
    BMC Syst Biol 6:70. 2012
    ..Yet, SCA1 mice show downregulated gene expression of IP3R1, Homer, metabotropic glutamate receptor (mGluR), smooth endoplasmic reticulum Ca-ATP-ase (SERCA), calbindin, parvalbumin, and other calcium signaling proteins...
  5. pmc Where does all the PIP2 come from?
    Leslie M Loew
    Richard D Berlin Center for Cell Analysis and Modelling, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Physiol 582:945-51. 2007
    ..This mechanism should be important not just for consideration of PIP(2) as a precursor of InsP(3), but for any pathway that can be directly or indirectly modulated by PIP(2)...
  6. ncbi request reprint The Virtual Cell: a software environment for computational cell biology
    L M Loew
    Center for Biomedical Imaging Technology, Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Trends Biotechnol 19:401-6. 2001
    ..The system enables the formulation of both compartmental and spatial models, the latter with either idealized or experimentally derived geometries of one, two or three dimensions...
  7. ncbi request reprint STAT module can function as a biphasic amplitude filter
    V Mayya
    Department of Cell Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington 06030, USA
    Syst Biol (Stevenage) 2:43-52. 2005
    ..We also demonstrate that maximal amplification at intermediate levels of STAT activation is a moderately robust property of STAT module. We propose that these observations can be extrapolated to the analogous SMAD signalling module...
  8. pmc Modeling and analysis of calcium signaling events leading to long-term depression in cerebellar Purkinje cells
    Nicholas Hernjak
    Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut
    Biophys J 89:3790-806. 2005
    ..Additionally, the calcium buffer capacity and diffusion rates from the spine are found to be important parameters in shaping the calcium spike...
  9. pmc Activation of phospholipase C increases intramembrane electric fields in N1E-115 neuroblastoma cells
    Chang Xu
    Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Biophys J 84:4144-56. 2003
    ..Our results suggest that the change in the local intramembrane potential induced by bradykinin may play a role in mediating the I(K(M)) inhibition...
  10. ncbi request reprint Geometry shapes cell signaling network output
    Raquell M Holmes
    University of Connecticut Health Center, Center for Cell Analysis and Modeling, Farmington, CT 06032 1507, USA
    Chem Biol 15:523-4. 2008
    ..2008) demonstrate that molecular interactions alone are insufficient to explain the patterns of MAPK activation: a model must include the cell morphology and spatial distribution of signaling molecules...
  11. ncbi request reprint The virtual cell: an integrated modeling environment for experimental and computational cell biology
    Ion I Moraru
    Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030 3505, USA
    Ann N Y Acad Sci 971:595-6. 2002
  12. ncbi request reprint A wave of IP3 production accompanies the fertilization Ca2+ wave in the egg of the frog, Xenopus laevis: theoretical and experimental support
    John Wagner
    Department of Physiology, Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, CT 06030 1507, USA
    Cell Calcium 35:433-47. 2004
    ..Since significant IP3 could not diffuse to this region from the sperm entry point within 2 min, this observation is consistent with a regenerative wave of IP3 production...
  13. ncbi request reprint Endogenous inhibitors of InsP3-induced Ca2+ release in neuroblastoma cells
    James Watras
    Department of Pharmacology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Brain Res 1055:60-72. 2005
    ..Thus, the low InsP3 sensitivity of Ca2+ release in neuroblastoma cells can be explained by the presence of cytosolic inhibitors of Ca2+ release and include stress-induced phosphoprotein 1...
  14. pmc Molecular machines or pleiomorphic ensembles: signaling complexes revisited
    Bruce J Mayer
    Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    J Biol 8:81. 2009
    ..Conventional mechanical descriptions misrepresent this reality and can be actively counterproductive by misdirecting us away from investigating critical issues...
  15. ncbi request reprint Intracellular signaling: spatial and temporal control
    Ion I Moraru
    Center for Cell Analysis and Modeling, Department of Cell Biology, University of Connecticut School of Medicine, Farmington, CT, USA
    Physiology (Bethesda) 20:169-79. 2005
    ..Cell physiology has passed the threshold: the time to begin modeling is now...
  16. ncbi request reprint Quantitative cell biology with the Virtual Cell
    Boris M Slepchenko
    Center For Biomedical Imaging Technology and National Resource for Cell Analysis and Modeling, Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Trends Cell Biol 13:570-6. 2003
    ..Applications to problems in nucleocytoplasmic transport and intracellular calcium dynamics will illustrate the power of this paradigm for elucidating cell biology...
  17. ncbi request reprint A fluorometric approach to local electric field measurements in a voltage-gated ion channel
    Osei Kwame Asamoah
    Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Neuron 37:85-97. 2003
    ..The extension of this method to other membrane bound proteins, including transporters, will yield insight into the role of electrical forces on protein function...
  18. pmc Analysis of phosphatidylinositol-4,5-bisphosphate signaling in cerebellar Purkinje spines
    Sherry Ann Brown
    R D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Biophys J 95:1795-812. 2008
    ..Simulation results indicate that local sequestration could explain the experimentally observed finely tuned timing between parallel fiber and climbing fiber activation...
  19. pmc Sensitivity of second harmonic generation from styryl dyes to transmembrane potential
    Andrew C Millard
    Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030 1507, USA
    Biophys J 86:1169-76. 2004
    ..We find a dependence of voltage sensitivity on excitation wavelength that is consistent with a two-photon resonance, and there is a significant dependence of voltage sensitivity on the structure of the nonchromophore portion of the dyes...
  20. pmc An open model of actin dendritic nucleation
    Jonathon A Ditlev
    Richard D Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030 1507, USA
    Biophys J 96:3529-42. 2009
    ..Because this model, with the simulation results, is "open source", in the sense that it is publicly available and editable through the Virtual Cell database (http://vcell.org), it can be accessed, analyzed, modified, and extended...
  21. ncbi request reprint The Virtual Cell project
    Leslie M Loew
    Center for Biomedical Imaging Technology, Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Novartis Found Symp 247:151-60; discussion 160-1, 198-206, 244-52. 2002
    ..Thus, communication between these traditionally separate scientific communities can be facilitated. This paper will describe the status of the project and will survey several applications to cell biological problems...
  22. ncbi request reprint Computational cell biology: spatiotemporal simulation of cellular events
    Boris M Slepchenko
    Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, CT 06117, USA
    Annu Rev Biophys Biomol Struct 31:423-41. 2002
    ..The theories behind both stochastic and deterministic simulations are discussed. Examples of respective applications to cell biological problems in RNA trafficking and neuronal calcium dynamics are provided to illustrate these ideas...
  23. pmc Dynamics of action potential backpropagation in basal dendrites of prefrontal cortical pyramidal neurons
    Wen Liang Zhou
    Department of Neuroscience, L 4000, UCONN Health Center, 263 Farmington Ave, Farmington, CT 06030 3401, USA
    Eur J Neurosci 27:923-36. 2008
    ..The majority of synaptic contacts in the basilar dendritic tree actually received significant AP-associated electrical and calcium transients...
  24. pmc Second harmonic imaging of exocytosis at fertilization
    Andrew C Millard
    Department of Cell Biology, University of Connecticut Health Center, Farmington, 06030 1507, USA
    Biophys J 88:L46-8. 2005
    ..With nonlinear imaging on a scanning microscope, we are able to visualize the wave of exocytosis in real time...
  25. ncbi request reprint Cooperativity between cell contractility and adhesion
    Igor L Novak
    Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Phys Rev Lett 93:268109. 2004
    ..The model predicts that the focal adhesions propagate by treadmilling with a velocity proportional to the integrin diffusion coefficient...
  26. ncbi request reprint Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells
    Andrew C Millard
    Center for Biomedical Imaging Technology, University of Connecticut Health Center, MC 1507, 263 Farmington Avenue, Farmington, Connecticut 06030 1507, USA
    Opt Lett 28:1221-3. 2003
    ..This sensitivity is significantly better than the optimal 10%/100 mV under fluorescence and further establishes the importance of second-harmonic generation for the functional imaging of membrane potential in living cells...
  27. pmc The effect of asymmetric surface potentials on the intramembrane electric field measured with voltage-sensitive dyes
    Chang Xu
    Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Biophys J 84:2768-80. 2003
    ..A surface charge density of 1 e(-)/115 A(2) provides the best fit and the intrinsic 1:1 association constants of Ba, Mg, and Ca with acidic group in the surface are 1.7, 6.1, and 25.3 M(-1)...
  28. ncbi request reprint Confocal and nonlinear optical imaging of potentiometric dyes
    Leslie M Loew
    Department of Physiology, Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Methods Cell Biol 70:429-52. 2002
  29. ncbi request reprint Cortically restricted production of IP3 leads to propagation of the fertilization Ca2+ wave along the cell surface in a model of the Xenopus egg
    Christopher P Fall
    Center for Neural Science, New York University, 4 Washington Place Room 809, New York, NY 10003, USA
    J Theor Biol 231:487-96. 2004
    ..Our model includes Ca2+ -dependent production of IP3 at the plasma membrane and IP3 degradation. Simulations in 1 dimension and axi-symmetric 3 dimensions illustrate the basic features of the wave...
  30. ncbi request reprint Systems analysis of Ran transport
    Alicia E Smith
    Center for Cell Signaling, Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
    Science 295:488-91. 2002
    ..Moreover, it provides the first estimate of the total in vivo flux (520 molecules per NPC per second and predicts that the transport system is robust...

Research Grants54

  1. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2009
    ..As the database of models expands, simulating drug actions and side effects will become increasingly feasible. ..
  2. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2005
    ..Calcium Dynamics aims to elucidate intracellular calcium signaling in cells via a systematic marriage of experiment and modeling approaches; ..
  3. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2005
    ..The goal of this aim is to determine if the apparently large receptor mediated changes in IEF can directly modulate membrane physiology ..
  4. CENTER FOR CELLULAR NANO-ORGANIZATION (RMI)
    Leslie Loew; Fiscal Year: 2004
    ..We anticipate that these studies will lead to new unifying principles on the initiation, self assembly and function of spatially organized intracellular nanostructures. ..
  5. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2004
    ..The goal of this aim is to determine if the apparently large receptor mediated changes in IEF can directly modulate membrane physiology ..
  6. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2006
    ..The goal of this aim is to determine if the apparently large receptor mediated changes in IEF can directly modulate membrane physiology ..
  7. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2006
    ..Calcium Dynamics aims to elucidate intracellular calcium signaling in cells via a systematic marriage of experiment and modeling approaches; ..
  8. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie M Loew; Fiscal Year: 2010
    ..As the database of models expands, simulating drug actions and side effects will become increasingly feasible. ..
  9. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie M Loew; Fiscal Year: 2010
    ..This technology will be applied to the study of normal and diseased heart. It will also be used to understand information processing in the brain at the level of a single synapse. ..
  10. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2009
    ..As the database of models expands, simulating drug actions and side effects will become increasingly feasible. ..
  11. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2009
    ..This technology will be applied to the study of normal and diseased heart. It will also be used to understand information processing in the brain at the level of a single synapse. ..
  12. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2007
    ..As the database of models expands, simulating drug actions and side effects will become increasingly feasible. ..
  13. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2007
    ..The goal of this aim is to determine if the apparently large receptor mediated changes in IEF can directly modulate membrane physiology ..
  14. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2004
    ..Calcium Dynamics aims to elucidate intracellular calcium signaling in cells via a systematic marriage of experiment and modeling approaches; ..
  15. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2003
    ..The goal of this aim is to determine if the apparently large receptor mediated changes in IEF can directly modulate membrane physiology ..
  16. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 1990
    ..The ratiometric approach will be used to probe for both resting and stimulated membrane potential changes during the cell cycle of HeLa cells...
  17. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 1999
    ..The spatial and dynamic interactions between these organelles within the differentiated neuroblastoma cell will be probed experimentally and analyzed with image-based modeling and simulation. ..
  18. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 1991
    ..The ratiometric approach will be used to probe for both resting and stimulated membrane potential changes during the cell cycle of HeLa cells...
  19. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 1992
    ..The ratiometric approach will be used to probe for both resting and stimulated membrane potential changes during the cell cycle of HeLa cells...
  20. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2003
    ..Calcium Dynamics aims to elucidate intracellular calcium signaling in cells via a systematic marriage of experiment and modeling approaches; ..
  21. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2002
    ..Calcium Dynamics aims to elucidate intracellular calcium signaling in cells via a systematic marriage of experiment and modeling approaches; ..
  22. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2002
    ..The spatial and dynamic interactions between these organelles within the differentiated neuroblastoma cell will be probed experimentally and analyzed with image-based modeling and simulation. ..
  23. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2001
    ..S. and Europe. Service, training, and dissemination activities of the Resource will all build upon the pre- existing facilities and track record of CBIT. ..
  24. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2001
    ..The spatial and dynamic interactions between these organelles within the differentiated neuroblastoma cell will be probed experimentally and analyzed with image-based modeling and simulation. ..
  25. NONLINEAR OPTICAL MICROSCOPE FOR CELL BIOL RESEARCH
    Leslie Loew; Fiscal Year: 2001
    ..Dr. Leslie Loew will use NLOM to perform optical recording of membrane electrical properties of neuronal cells in acute brain ..
  26. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 2000
    ..S. and Europe. Service, training, and dissemination activities of the Resource will all build upon the pre- existing facilities and track record of CBIT. ..
  27. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 2000
    ..The spatial and dynamic interactions between these organelles within the differentiated neuroblastoma cell will be probed experimentally and analyzed with image-based modeling and simulation. ..
  28. NATIONAL RESOURCE FOR CELL ANALYSIS AND MODELING
    Leslie Loew; Fiscal Year: 1999
    ..S. and Europe. Service, training, and dissemination activities of the Resource will all build upon the pre- existing facilities and track record of CBIT. ..
  29. POTENTIOMETRIC DYES AND MEMBRANE PERMEABILITY
    Leslie Loew; Fiscal Year: 1993
    ..The ratiometric approach will be used to probe for both resting and stimulated membrane potential changes during the cell cycle of HeLa cells...