Guozheng Liu

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. pmc A novel pretargeting method for measuring antibody internalization in tumor cells
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Cancer Biother Radiopharm 22:33-9. 2007
  2. ncbi request reprint Optical pretargeting of tumor with fluorescent MORF oligomers
    Jiang He
    Department of Radiology, University of California San Francisco, San Francisco, CA 94143, USA
    Mol Imaging Biol 9:17-23. 2007
  3. pmc Pretargeting vs. direct targeting of human betalox5 islet cells subcutaneously implanted in mice using an anti-human islet cell antibody
    Guozheng Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Biol 39:645-51. 2012
  4. pmc Preparation of (111)In-DTPA morpholino oligomer for low abdominal accumulation
    Guozheng Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Appl Radiat Isot 68:1709-14. 2010
  5. pmc Further investigations of morpholino pretargeting in mice--establishing quantitative relations in tumor
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0243, USA
    Eur J Nucl Med Mol Imaging 32:1115-23. 2005
  6. pmc The ratio of maximum percent tumour accumulations of the pretargeting agent and the radiolabelled effector is independent of tumour size
    Guozheng Liu
    Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, MA01655 0243, United States
    Eur J Cancer 45:3098-103. 2009
  7. pmc Replacing 99mTc with 111In improves MORF/cMORF pretargeting by reducing intestinal accumulation
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655 0243, USA
    Mol Imaging Biol 11:303-7. 2009
  8. pmc An experimental and theoretical evaluation of the influence of pretargeting antibody on the tumor accumulation of effector
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0243, USA
    Mol Cancer Ther 7:1025-32. 2008
  9. pmc Radiolabeling of MAG3-morpholino oligomers with 188Re at high labeling efficiency and specific radioactivity for tumor pretargeting
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Appl Radiat Isot 64:971-8. 2006
  10. pmc Pretargeting CWR22 prostate tumor in mice with MORF-B72.3 antibody and radiolabeled cMORF
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Eur J Nucl Med Mol Imaging 35:272-80. 2008

Research Grants

Detail Information

Publications60

  1. pmc A novel pretargeting method for measuring antibody internalization in tumor cells
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Cancer Biother Radiopharm 22:33-9. 2007
    ..This method of measuring antibody internalization is directly applicable to pretargeting applications but can be a universal alternative to the conventional acid-wash method, with the advantage of leaving the cell membrane undamaged...
  2. ncbi request reprint Optical pretargeting of tumor with fluorescent MORF oligomers
    Jiang He
    Department of Radiology, University of California San Francisco, San Francisco, CA 94143, USA
    Mol Imaging Biol 9:17-23. 2007
    ..The use of linear fluorophore-conjugated oligomer duplexes have been little used in animals, and to our knowledge, have not previously been considered for pretargeting applications...
  3. pmc Pretargeting vs. direct targeting of human betalox5 islet cells subcutaneously implanted in mice using an anti-human islet cell antibody
    Guozheng Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Biol 39:645-51. 2012
    ..We now compare pretargeting with direct targeting in the beta cell transplant model to evaluate the degree to which target/non-target (T/NT) ratios may be improved by pretargeting...
  4. pmc Preparation of (111)In-DTPA morpholino oligomer for low abdominal accumulation
    Guozheng Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Appl Radiat Isot 68:1709-14. 2010
    ....
  5. pmc Further investigations of morpholino pretargeting in mice--establishing quantitative relations in tumor
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0243, USA
    Eur J Nucl Med Mol Imaging 32:1115-23. 2005
    ..In the present study, the pharmacokinetics of the antibody and effector were measured under different conditions in mice to establish their quantitative relationships with tumor accumulations by pretargeting...
  6. pmc The ratio of maximum percent tumour accumulations of the pretargeting agent and the radiolabelled effector is independent of tumour size
    Guozheng Liu
    Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, MA01655 0243, United States
    Eur J Cancer 45:3098-103. 2009
    ..Theoretical considerations also suggest that this relationship may be universal for alternative antibody/effector pairs and for different target models, but this must be experimentally confirmed...
  7. pmc Replacing 99mTc with 111In improves MORF/cMORF pretargeting by reducing intestinal accumulation
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655 0243, USA
    Mol Imaging Biol 11:303-7. 2009
    ..To reduce accumulation in the abdomen by MORF/cMORF pretargeting, 111In was compared to 99mTc as the radiolabel...
  8. pmc An experimental and theoretical evaluation of the influence of pretargeting antibody on the tumor accumulation of effector
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0243, USA
    Mol Cancer Ther 7:1025-32. 2008
    ..This conclusion will apply equally well to effectors carrying a fluorescent probe, an anticancer agent, or a radioactive imaging agent...
  9. pmc Radiolabeling of MAG3-morpholino oligomers with 188Re at high labeling efficiency and specific radioactivity for tumor pretargeting
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Appl Radiat Isot 64:971-8. 2006
    ..A labeling efficiency of greater than 90% can be achieved even at a concentration of MAG(3)-cMORF as low as 0.8 microM. The labeled cMORF is stable and capable of hybridizing to its complement...
  10. pmc Pretargeting CWR22 prostate tumor in mice with MORF-B72.3 antibody and radiolabeled cMORF
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Eur J Nucl Med Mol Imaging 35:272-80. 2008
    ..We have now applied our MORF/cMORF pretargeting technology to the targeting of CWR22 prostate tumor in nude mice...
  11. pmc Predicting the biodistribution of radiolabeled cMORF effector in MORF-pretargeted mice
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0243, USA
    Eur J Nucl Med Mol Imaging 34:237-46. 2007
    ....
  12. pmc Labeling biomolecules with radiorhenium: a review of the bifunctional chelators
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Anticancer Agents Med Chem 7:367-77. 2007
    ....
  13. pmc Human islet cell MORF/cMORF pretargeting in a xenogeneic murine transplant model
    Guozheng Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Mol Pharm 8:767-73. 2011
    ..Thus MORF/cMORF pretargeting may be useful to measure noninvasively human islet cell mass within the pancreas or following islet transplantation...
  14. pmc Tumor pretargeting in mice using MORF conjugated CC49 antibody and radiolabeled complimentary cMORF effector
    G Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Q J Nucl Med Mol Imaging 54:333-40. 2010
    ..This investigation evaluated the antiTAG-72 antibody CC49 as an alternative to MN14 for this application...
  15. pmc Unexpected side products in the conjugation of an amine-derivatized morpholino oligomer with p-isothiocyanate benzyl DTPA and their removal
    Guozheng Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Biol 38:159-63. 2011
    ..Adding a postconjugation-prepurification heating step dissociated the side products, improved the label stability and lowered tissue backgrounds in mice...
  16. pmc 90Y labeled phosphorodiamidate morpholino oligomer for pretargeting radiotherapy
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0243, United States
    Bioconjug Chem 22:2539-45. 2011
    ..Because of the longer physical half-life, the T/NT absorbed radiation dose ratios were improved in most organs and especially in blood...
  17. pmc A preclinical 188Re tumor therapeutic investigation using MORF/cMORF pretargeting and an antiTAG-72 antibody CC49
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Cancer Biol Ther 10:767-74. 2010
    ..In conclusion, effective radiotherapy was achieved in a mouse model by MORF/cMORF pretargeting using 188Re as the therapeutic radionuclide and CC49 as the pretargeting antibody...
  18. ncbi request reprint 99mTc-N, N'-bis(aminoethyl)propanediamine hexaacetic acid (BPHA): a glomerular filtration agent similar to 99mTc-DTPA
    Guozheng Liu
    Isotope Department, China Institute of Atomic Energy, Beijing, Peoples Republic of China
    Nucl Med Biol 29:399-404. 2002
    ..Excellent kidney images both in rabbits and a human volunteer were obtained. The significance of higher GFR estimates using 99mTc-BPHA compared to 99mTc-DTPA is discussed...
  19. pmc Adding a clearing agent to pretargeting does not lower the tumor accumulation of the effector as predicted
    Guozheng Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, 01655 0243, USA
    Cancer Biother Radiopharm 25:757-62. 2010
    ..30% ID/g as predicted. In conclusion, in addition to the expected reduced effector levels in blood and normal tissues, a reduction in tumor accumulation was avoided when adding a clearing agent as predicted...
  20. ncbi request reprint Amplification targeting: a modified pretargeting approach with potential for signal amplification-proof of a concept
    Jiang He
    Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Nucl Med 45:1087-95. 2004
    ..Amplification targeting is a multistep procedure with the potential to greatly improve target localization of radioactivity (and eventually drugs) through the intermediate use of polymers conjugated with multiple copies of oligomers...
  21. pmc Identification of a high affinity TAG-72 binding peptide by phage display selection
    Nan Xiao
    University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Cancer Biol Ther 11:22-31. 2011
    ..Phage display was used to select novel peptides that specifically bind the TAG-72 antigen and with properties suitable for imaging TAG-72 positive cancers...
  22. ncbi request reprint Pretargeting in tumored mice with radiolabeled morpholino oligomer showing low kidney uptake
    Guozheng Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, Worcester, USA
    Eur J Nucl Med Mol Imaging 31:417-24. 2004
    ..The lowering of kidney radioactivity levels in this way may be important to reduce toxicity to this organ in connection with pretargeting radiotherapy studies...
  23. doi request reprint Comparing the intracellular fate of components within a noncovalent streptavidin nanoparticle with covalent conjugation
    Yuxia Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Biol 39:101-7. 2012
    ..We hypothesize that the noncovalent nature of a streptavidin/biotin three-component nanoparticle possessing these qualities may be required for dissociation of the radiolabeled oligomer and its accumulation into the cell nucleus...
  24. ncbi request reprint Radiolabelling morpholinos with 188Re tricarbonyl provides improved in vitro and in vivo stability to re-oxidation
    Jiang He
    Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Commun 25:731-6. 2004
    ..However, by preparing 188Re-MORFs labelled conventionally with MAG3 as chelator, we have observed unacceptable levels of oxidation to perrhenate in vitro and in vivo in mice...
  25. pmc A new TAG-72 cancer marker peptide identified by phage display
    Ling Chen
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Cancer Lett 272:122-32. 2008
    ..These data indicate that the A2-6 peptide is specific for the TAG-72 cancer target...
  26. pmc Successful radiotherapy of tumor in pretargeted mice by 188Re-radiolabeled phosphorodiamidate morpholino oligomer, a synthetic DNA analogue
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Clin Cancer Res 12:4958-64. 2006
    ..Our laboratory has reported on MORF pretargeting for diagnosis using (99m)Tc as radiolabel. We now report on the use of MORF pretargeting for radiotherapy in a mouse tumor model using (188)Re as the therapeutic radiolabel...
  27. doi request reprint A convenient thiazole orange fluorescence assay for the evaluation of DNA duplex hybridization stability
    Minmin Liang
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Imaging Biol 11:439-45. 2009
    ..This assay was developed primarily to screen antisense oligomer duplexes that are stable in serum and in the cytoplasm but unstable in the presence of their target messenger RNA (mRNA)...
  28. doi request reprint Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
    Minmin Liang
    Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 21:1385-8. 2010
    ..Presumably, other imaging modalities could also be considered...
  29. ncbi request reprint Improved delivery in cell culture of radiolabeled antisense DNAs by duplex formation
    Xinrong Liu
    Department of Radiology, Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, 01655, USA
    Mol Imaging Biol 8:278-83. 2006
    ..We have investigated the mechanism involved to evaluate whether the approach may be useful for antisense radionuclide imaging...
  30. ncbi request reprint Construction of a novel chimera consisting of a chelator-containing Tat peptide conjugated to a morpholino antisense oligomer for technetium-99m labeling and accelerating cellular kinetics
    Yu Min Zhang
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Biol 33:263-9. 2006
    ....
  31. doi request reprint Optical antisense tumor targeting in vivo with an improved fluorescent DNA duplex probe
    Minmin Liang
    Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Bioconjug Chem 20:1223-7. 2009
    ..In conclusion, by a process designed to optimize the duplex for optical antisense tumor targeting, the fluorescence signal was improved both in cells and in tumored mice...
  32. pmc Comparing two TAG-72 binding peptides previously identified by phage display as potential imaging agents
    Ling Chen
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Nucl Med Commun 32:920-4. 2011
    ..To evaluate the targeting property in vitro and in vivo of two tumor-associated glycoprotein 72 (TAG-72) binding peptides, previously identified in this laboratory by phage selection using different elution conditions...
  33. pmc Comparison of several linear fluorophore- and quencher-conjugated oligomer duplexes for stability, fluorescence quenching, and kinetics in vitro and in vivo in mice
    Surong Zhang
    Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 18:1170-5. 2007
    ..5 may be efficiently and rapidly quenched by Iowa Black when both are conjugated to complementary oligomers and that the resulting inhibition of fluorescence is sufficiently persistent for imaging...
  34. ncbi request reprint Evaluation of 99mTc-MAG3-annexin V: influence of the chelate on in vitro and in vivo properties in mice
    Jean Luc Vanderheyden
    Department of Radiology, University of Massachusetts Medical School, Worcester, 01655, USA
    Nucl Med Biol 33:135-44. 2006
    ..Nevertheless, (99m)Tc MAG(3)-annexin may be an attractive alternative to (99m)Tc Hynic-annexin for the in vivo imaging of phosphatidylserine receptors...
  35. ncbi request reprint Pharmacokinetics in mice of four oligomer-conjugated polymers for amplification targeting
    Jiang He
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA, USA
    Cancer Biother Radiopharm 18:941-7. 2003
    ..In particular, the polymer must remain in circulation sufficiently long to permit target localization...
  36. ncbi request reprint Tumor pretargeting in mice using (99m)Tc-labeled morpholino, a DNA analog
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0243, USA
    J Nucl Med 43:384-91. 2002
    ..MORFs support the nitrogenous bases by nonionic phosphorodiamidate linkages and, besides being nuclease resistant, can display good water solubility...
  37. pmc Synthesis and in vitro characterization of a dendrimer-MORF conjugate for amplification pretargeting
    Xiangji Chen
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 19:1518-25. 2008
    ..Further amplification studies using the CC49-(c)MORF for LS174T tumor cells in tissue culture also demonstrated clear evidence of signal amplification...
  38. pmc Affinity enhancement pretargeting: synthesis and testing of a 99mTc-labeled bivalent MORF
    Jiang He
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Mol Pharm 7:1118-24. 2010
    ..Thus two monovalent MORFs were covalently conjugated into a bivalent MORF effector to improve tumor targeting by both pharmacokinetics and affinity enhancement influences...
  39. ncbi request reprint Optical antisense imaging of tumor with fluorescent DNA duplexes
    Xinrong Liu
    Depatment of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Bioconjug Chem 18:1905-11. 2007
    ..This proof of concept investigation of optical antisense targeting therefore suggests that further studies including optimization of this approach are appropriate...
  40. pmc Detection of Aspergillus fumigatus pulmonary fungal infections in mice with (99m)Tc-labeled MORF oligomers targeting ribosomal RNA
    Yuzhen Wang
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Nucl Med Biol 40:89-96. 2013
    ..In this study, we investigated (99m)Tc labeled MORF oligomers targeting fungal ribosomal RNA (rRNA) for the imaging detection of fungal infections...
  41. doi request reprint Reducing the background fluorescence in mice receiving fluorophore/inhibitor DNA duplexes
    Minmin Liang
    Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Mol Pharm 8:126-32. 2011
    ..05) background fluorescence after administration to SKH-1 normal mice and apparently without affecting target mRNA binding in vitro in cell culture or in vivo in tumor bearing mice...
  42. ncbi request reprint Cytosine residues influence kidney accumulations of 99mTc-labeled morpholino oligomers
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Antisense Nucleic Acid Drug Dev 12:393-8. 2002
    ..These results show that the base sequences of MORFs, and probably other oligomers as well, are an important determinant of biodistribution...
  43. ncbi request reprint Improving the labeling of S-acetyl NHS-MAG(3)-conjugated morpholino oligomers
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 13:893-7. 2002
    ....
  44. ncbi request reprint Initial observations of 99mTc labelled locked nucleic acids for antisense targeting
    Yumin Zhang
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA, USA
    Nucl Med Commun 25:1113-8. 2004
    ..The most recent DNA analogues to become commercially available are known as locked nucleic acids (LNAs). The aim of this study was to evaluate the properties of LNAs for antisense targeting...
  45. ncbi request reprint Affinity enhancement bivalent morpholinos for pretargeting: surface plasmon resonance studies of molecular dimensions
    Jiang He
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 16:1098-104. 2005
    ..The results provide further evidence that the use of bivalent effectors can greatly improve MORF pretargeting and, finally, that bivalent MORFs with reduced equilibrium constants may actually exhibit higher affinity enhancement...
  46. ncbi request reprint Nonspecific cellular accumulation of 99mTc-labeled oligonucleotides in culture is influenced by their guanine content
    Yi Wang
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Biol 34:47-54. 2007
    ..If this observation can be shown to be universally applicable to other cell types, then the selection of control sequences in studies of antisense tumor targeting should avoid those that are guanine rich, if possible...
  47. ncbi request reprint A brief evaluation of tumor imaging in mice with 99mTc-glucarate including a comparison with 18F-FDG
    Dengfeng Cheng
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Curr Radiopharm 4:5-9. 2011
    ....
  48. doi request reprint Improving the quantitation accuracy in noninvasive small animal single photon emission computed tomography imaging
    Dengfeng Cheng
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Biol 38:843-8. 2011
    ....
  49. ncbi request reprint In vitro and in vivo evaluation of a Technetium-99m-labeled cyclic RGD peptide as a specific marker of alpha(V)beta(3) integrin for tumor imaging
    Zi Fen Su
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, 01655, USA
    Bioconjug Chem 13:561-70. 2002
    ..In conclusion, possibly because of limited numbers of alpha(V)beta(3) integrin receptors per tumor cell and low binding affinity, radiolabeled RGD peptides may have limitations as tumor imaging agents...
  50. pmc Investigation of four (99m)Tc-labeled bacteriophages for infection-specific imaging
    Mary Rusckowski
    Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Nucl Med Biol 35:433-40. 2008
    ..Previously, a (99m)Tc-labeled M13 phage demonstrated specific binding for its host Escherichia coli in vitro and in mice through imaging...
  51. ncbi request reprint Investigations of a (99m)Tc-labeled bacteriophage as a potential infection-specific imaging agent
    Mary Rusckowski
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Nucl Med 45:1201-8. 2004
    ..Because bacteriophages (phages) have a natural specificity for bacteria, it may be possible to develop radiolabeled phages as infection-specific agents...
  52. pmc An improved method for covalently conjugating morpholino oligomers to antitumor antibodies
    Jiang He
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 18:983-8. 2007
    ..Using MN14(SANH)-MORF, the influence of the higher gpm on pretargeting permitted lowering the dosages of MN14 administered and may permit administering higher levels of radioactivity in connection with therapy...
  53. ncbi request reprint A comparison of in vitro and in vivo stability in mice of two morpholino duplexes differing in chain length
    Jiang He
    Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 14:1018-23. 2003
    ..The stability of hybridized duplexes is an important criterion for any radiopharmaceutical application of DNAs or their analogues such as phosphorodiamidate morpholinos (MORFs)...
  54. ncbi request reprint Evidence of specificity of radiolabeled phage display peptides for the TAG-72 antigen
    Mary Rusckowski
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655 0243, USA
    Cancer Biother Radiopharm 22:564-72. 2007
    ..The results suggest that this peptide when free of the phage may have potential for the imaging and possibly radiotherapy of TAG-72-expressing cancers...
  55. ncbi request reprint Affinity enhancement bivalent morpholino for pretargeting: initial evidence by surface plasmon resonance
    Jiang He
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 16:338-45. 2005
    ..These results show that bimolecular binding was occurring in the case of the bivalent MORF and suggest that bivalency may be superior to monovalency in MORF pretargeting applications...
  56. ncbi request reprint Initial mechanistic studies of antisense targeting in cells
    Xinrong Liu
    Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Nucl Med 47:360-8. 2006
    ..The continued development of antisense targeting will require a better understanding of the mechanism...
  57. pmc Preparation and properties of 99mTc(CO)3+-labeled N,N-bis(2-pyridylmethyl)-4-aminobutyric acid
    Guozheng Liu
    Department of Radiology, Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 15:1441-6. 2004
    ..Finally, the complex (99m)Tc(CO)(3)(+)-BPABA itself was found to clear exclusively via the hepatobiliary pathway and may have value as a potential hepatobiliary imaging agent...
  58. ncbi request reprint Nitriles form mixed-coligand complexes with (99m)Tc-HYNIC-peptide
    Guozheng Liu
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nucl Med Biol 29:107-13. 2002
    ..Furthermore, the presence of acetonitrile during Sep-Pak or HPLC purification may inadvertently generate a mixed tricine/acetonitile coligand (99m)Tc-HYNIC-peptide complex...
  59. ncbi request reprint Methods for MAG3 conjugation and 99mTc radiolabeling of biomolecules
    Yi Wang
    Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Nat Protoc 1:1477-80. 2006
    ..Apart from the preparation of reagents, the conjugation and purification requires about 4 h, while the labeling with 99mTc requires about an additional 30 min...
  60. pmc A semiempirical model of tumor pretargeting
    Guozheng Liu
    Department of Radiology, Division of Nuclear Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Bioconjug Chem 19:2095-104. 2008
    ..Several misconceptions that often surround pretargeting are also clarified...

Research Grants1

  1. Pretargeting mouse tumor with anti-PSMA antibody-MORF
    Guozheng Liu; Fiscal Year: 2005
    ..Based on our experiences in this pretargeting strategy, we are confident that these investigations on the pretargeting imaging of prostate cancer models with MORFs will be successful. ..