J J LiPuma

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint An epidemic Burkholderia cepacia complex strain identified in soil
    John J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, 8323 MSRB III, Box 0646, Ann Arbor, MI 48109, USA
    Lancet 359:2002-3. 2002
  2. pmc Using BOX-PCR to exclude a clonal outbreak of melioidosis
    Bart J Currie
    Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia
    BMC Infect Dis 7:68. 2007
  3. pmc Spontaneous and evolutionary changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis
    Andrea Sass
    Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, CF10 3AT, UK
    BMC Genomics 12:373. 2011
  4. ncbi request reprint Update on the Burkholderia cepacia complex
    John J LiPuma
    Division of Infectious Diseases, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    Curr Opin Pulm Med 11:528-33. 2005
  5. pmc In vitro activities of a novel nanoemulsion against Burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species
    John J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109, USA
    Antimicrob Agents Chemother 53:249-55. 2009
  6. pmc The changing microbial epidemiology in cystic fibrosis
    John J LiPuma
    Department of Pediatrics and Communicable Diseases, 1150 W Medical Center Drive, 8323 MSRB III, SPC 5646, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Clin Microbiol Rev 23:299-323. 2010
  7. ncbi request reprint Burkholderia cepacia complex: a contraindication to lung transplantation in cystic fibrosis?
    J J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    Transpl Infect Dis 3:149-60. 2001
  8. ncbi request reprint Disproportionate distribution of Burkholderia cepacia complex species and transmissibility markers in cystic fibrosis
    J J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    Am J Respir Crit Care Med 164:92-6. 2001
  9. ncbi request reprint Microbiological and immunologic considerations with aerosolized drug delivery
    J J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI, USA
    Chest 120:118S-123S. 2001
  10. pmc Distribution of Burkholderia cepacia complex species among isolates recovered from persons with or without cystic fibrosis
    Rebecca Reik
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 1150 W Medical Center Dr, 8323 MSRB III, Box 0646, Ann Arbor, MI 48109 0646, USA
    J Clin Microbiol 43:2926-8. 2005

Research Grants

Collaborators

Detail Information

Publications73

  1. ncbi request reprint An epidemic Burkholderia cepacia complex strain identified in soil
    John J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, 8323 MSRB III, Box 0646, Ann Arbor, MI 48109, USA
    Lancet 359:2002-3. 2002
    ..This finding indicates that human pathogenic strains are not necessarily distinct from environmental strains, and might help explain ongoing human acquisition despite strict infection control measures...
  2. pmc Using BOX-PCR to exclude a clonal outbreak of melioidosis
    Bart J Currie
    Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia
    BMC Infect Dis 7:68. 2007
    ..pseudomallei isolates, but they are time consuming and technically challenging for many laboratories...
  3. pmc Spontaneous and evolutionary changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis
    Andrea Sass
    Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, CF10 3AT, UK
    BMC Genomics 12:373. 2011
    ..Two pan-resistant ET12 outbreak isolates recovered two decades after J2315 were also compared to identify naturally evolved gene expression changes...
  4. ncbi request reprint Update on the Burkholderia cepacia complex
    John J LiPuma
    Division of Infectious Diseases, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    Curr Opin Pulm Med 11:528-33. 2005
    ....
  5. pmc In vitro activities of a novel nanoemulsion against Burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species
    John J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109, USA
    Antimicrob Agents Chemother 53:249-55. 2009
    ..These results support NB-401's potential role as a novel antimicrobial agent for the treatment of infection due to CF-related opportunistic pathogens...
  6. pmc The changing microbial epidemiology in cystic fibrosis
    John J LiPuma
    Department of Pediatrics and Communicable Diseases, 1150 W Medical Center Drive, 8323 MSRB III, SPC 5646, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Clin Microbiol Rev 23:299-323. 2010
    ..The challenges inherent in tracking and interpreting rates of infection in this patient population are discussed...
  7. ncbi request reprint Burkholderia cepacia complex: a contraindication to lung transplantation in cystic fibrosis?
    J J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    Transpl Infect Dis 3:149-60. 2001
    ..Additional study is needed to better define the relative risks associated with each species of the B. cepacia complex...
  8. ncbi request reprint Disproportionate distribution of Burkholderia cepacia complex species and transmissibility markers in cystic fibrosis
    J J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    Am J Respir Crit Care Med 164:92-6. 2001
    ..cepacia complex. The low frequency of BCESM and cblA suggests that they are not sufficient markers of B. cepacia virulence or transmissibility...
  9. ncbi request reprint Microbiological and immunologic considerations with aerosolized drug delivery
    J J LiPuma
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI, USA
    Chest 120:118S-123S. 2001
    ..Increases in Stenotrophomonas maltophilia were detected in patients after 18 and 24 months of tobramycin therapy and were similar to those rates in patients receiving placebo. These rates may be independent of inhalation therapy...
  10. pmc Distribution of Burkholderia cepacia complex species among isolates recovered from persons with or without cystic fibrosis
    Rebecca Reik
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 1150 W Medical Center Dr, 8323 MSRB III, Box 0646, Ann Arbor, MI 48109 0646, USA
    J Clin Microbiol 43:2926-8. 2005
    ..Although all B. cepacia complex species were found, some were rarely identified. The distribution of species differed between the CF and non-CF populations and appears to be changing over time among CF patients...
  11. pmc Recovery of Herbaspirillum species from persons with cystic fibrosis
    Theodore Spilker
    Division of Infectious Diseases, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    J Clin Microbiol 46:2774-7. 2008
    ..Although the role that these species play in lung disease in persons with CF is not known, their differentiation from other species is important and has serious implications for clinical care and patient well-being...
  12. pmc Identification by subtractive hybridization of a novel insertion element specific for two widespread Burkholderia cepacia genomovar III strains
    Lixia Liu
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    J Clin Microbiol 41:2471-6. 2003
    ....
  13. pmc PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients
    Theodore Spilker
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, 48109 Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium
    J Clin Microbiol 42:2074-9. 2004
    ..Thus, we have designed two PCR assays: one is specific for the genus Pseudomonas, while the other is specific for P. aeruginosa. Both assays show 100% sensitivity and specificity...
  14. pmc Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109 0646, USA
    J Clin Microbiol 40:3300-7. 2002
    ..e., local epidemiology), whereas BOX-PCR fingerprinting is more appropriate for large-scale studies (i.e., global epidemiology). In this regard, BOX-PCR fingerprinting can be considered a rapid and easy alternative to MLRT...
  15. pmc Ribosomal DNA-directed PCR for identification of Achromobacter (Alcaligenes) xylosoxidans recovered from sputum samples from cystic fibrosis patients
    Lixia Liu
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Clin Microbiol 40:1210-3. 2002
    ..The availability of this assay will enhance identification of A. xylosoxidans, thereby facilitating study of the pathogenic role of this species and improving infection control efforts in CF...
  16. ncbi request reprint Multilocus restriction typing: a novel tool for studying global epidemiology of Burkholderia cepacia complex infection in cystic fibrosis
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109 0646, USA
    J Infect Dis 185:1454-62. 2002
    ..The portability of MLRT data will facilitate comparison of data obtained in different laboratories. In addition, data obtained with MLRT can be used in studies of bacterial population structure...
  17. ncbi request reprint Ralstonia respiraculi sp. nov., isolated from the respiratory tract of cystic fibrosis patients
    Tom Coenye
    Department of Pediatrics, University of Michigan, 1150 W Med Ctr Dr, Ann Arbor, MI 48109 0646, USA
    Int J Syst Evol Microbiol 53:1339-42. 2003
    ..nov. is proposed; the other isolate was phylogenetically closely related to R. respiraculi, but is likely to represent another novel Ralstonia species. The type strain of R. respiraculi is AU3313(T) (=LMG 21510(T)=CCUG 46809(T))...
  18. ncbi request reprint Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan, 1150 W Med Ctr Dr, MSRB III, Rm 8323, Ann Arbor, MI 48109 0646, USA
    Int J Syst Evol Microbiol 53:1075-80. 2003
    ..Some outstanding issues in the taxonomy of the genus Ralstonia are also discussed...
  19. pmc Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Clin Microbiol 40:2062-9. 2002
    ....
  20. ncbi request reprint Simultaneous coinfection by multiple strains during Burkholderia cepacia complex infection in cystic fibrosis
    Jeffrey H Yang
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, USA
    Diagn Microbiol Infect Dis 54:95-8. 2006
    ..Transient coinfection with more than 1 strain occurs infrequently and may be more common early in the course of Bcc infection...
  21. doi request reprint Induction of immune response to the 17 kDa OMPA Burkholderia cenocepacia polypeptide and protection against pulmonary infection in mice after nasal vaccination with an OMP nanoemulsion-based vaccine
    P E Makidon
    Michigan Nanotechnology Institute for Medicine and Biological Sciences MNIMBS, University of Michigan, 9220 MSRB III, 1150 W Medical Center Dr, Ann Arbor, MI, 48109 5648, USA
    Med Microbiol Immunol 199:81-92. 2010
    ..cenocepacia. We have also identified that a 17 kDa OmpA-like protein highly conserved between Burkholderia and Ralstonia species as a new immunodominant epitope in mucosal immunization...
  22. doi request reprint Identification of Bordetella spp. in respiratory specimens from individuals with cystic fibrosis
    T Spilker
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Clin Microbiol Infect 14:504-6. 2008
    ..Consideration should be given to the presence of these organisms in the evaluation of CF sputum cultures...
  23. ncbi request reprint Evidence of transmission of Burkholderia cepacia, Burkholderia multivorans and Burkholderia dolosa among persons with cystic fibrosis
    Rhiannon Biddick
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI, USA
    FEMS Microbiol Lett 228:57-62. 2003
    ..These observations suggest that B. cepacia complex strains in species other than B. cenocepacia may be spread among CF patients...
  24. pmc Expanded multilocus sequence typing for burkholderia species
    Theodore Spilker
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, 48109 0646, USA
    J Clin Microbiol 47:2607-10. 2009
    ..cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing...
  25. ncbi request reprint Burkholderia cepacia complex in cystic fibrosis: frequency of strain replacement during chronic infection
    Scott A Bernhardt
    Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MI 48109 0646, USA
    Clin Infect Dis 37:780-5. 2003
    ..cepacia complex strain. The potential for strain replacement during chronic infection may confound studies of the relationship between strain and clinical outcome and must be considered in designing effective infection-control practices...
  26. pmc Burkholderia cenocepacia ET12 strain activates TNFR1 signalling in cystic fibrosis airway epithelial cells
    Umadevi S Sajjan
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 1150 W Medical Center Drive, Ann Arbor, MI 48109, USA
    Cell Microbiol 10:188-201. 2008
    ..cenocepacia, ET12 strain. Together, these results suggest that interaction of B. cenocepacia, ET12 strain with TNFR1 may contribute to robust inflammatory responses elicited by this organism...
  27. ncbi request reprint Population structure analysis of Burkholderia cepacia genomovar III: varying degrees of genetic recombination characterize major clonal complexes
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, USA
    Microbiology 149:77-88. 2003
    ..The observation that genetic recombination is frequent within some B. cepacia genomovar III populations has important implications for the biotechnological use of B. cepacia complex species...
  28. pmc Infection by Ralstonia species in cystic fibrosis patients: identification of R. pickettii and R. mannitolilytica by polymerase chain reaction
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, 48109 0646, USA
    Emerg Infect Dis 8:692-6. 2002
    ..To facilitate identification of R. mannitolilytica and R. pickettii, we developed 16S ribosomal DNA-based polymerase chain reaction assays that allow sensitive and specific identification of these species...
  29. ncbi request reprint Use of the gyrB gene for the identification of Pandoraea species
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 8301 MSRB III, Box 0646, 1150 W Med Ctr Dr, Ann Arbor, MI 48109 0646, USA
    FEMS Microbiol Lett 208:15-9. 2002
    ..Our data also indicate that the taxonomic status of Pandoraea genomospecies 2 should be reinvestigated...
  30. pmc Rhinovirus infection liberates planktonic bacteria from biofilm and increases chemokine responses in cystic fibrosis airway epithelial cells
    Sangbrita S Chattoraj
    Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA
    Thorax 66:333-9. 2011
    ..These observations imply that acute RV infection may increase lower respiratory symptoms by increasing planktonic bacterial load. However, the underlying mechanisms are not known...
  31. pmc Burkholderia glumae infection in an infant with chronic granulomatous disease
    Jason B Weinberg
    Division of Infectious Deseases, Department of Pediatrics and Communicable Deseases, University of Michigan Medical School, Ann Arbor, MI, USA
    J Clin Microbiol 45:662-5. 2007
    ..While other Burkholderia species have been identified as important pathogens in persons with CGD, B. glumae has not been previously reported to cause human infection...
  32. pmc A type IV secretion system contributes to intracellular survival and replication of Burkholderia cenocepacia
    S Umadevi Sajjan
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Infect Immun 76:5447-55. 2008
    ..cenocepacia is similar in both professional and nonprofessional phagocytes and that a functional plasmid-encoded type IV secretion system contributes to the survival and replication of B. cenocepacia in eukaryotic cells...
  33. ncbi request reprint Molecular epidemiology of Burkholderia species
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, USA
    Front Biosci 8:e55-67. 2003
    ..cepacia. How these species, collectively referred to as the B. cepacia complex, differ with respect to their epidemiology, natural history, and pathology in CF is the subject of ongoing investigation...
  34. pmc Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays
    T Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, USA
    J Clin Microbiol 39:4452-5. 2001
    ..The use of these PCR assays facilitates the identification of Pandoraea spp. and avoids the misidentification of a Pandoraea sp. as a B. cepacia complex isolate...
  35. ncbi request reprint Intracellular trafficking and replication of Burkholderia cenocepacia in human cystic fibrosis airway epithelial cells
    Umadevi S Sajjan
    Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, 48109, USA
    Cell Microbiol 8:1456-66. 2006
    ..This survival and replication strategy may contribute to the capacity of B. cenocepacia to persist in the lungs of infected CF patients...
  36. pmc Disruption of tight junctions during traversal of the respiratory epithelium by Burkholderia cenocepacia
    Jason Y Kim
    Department of Pediatrics, University of Michigan Medical School, 1150 W Medical Center Dr, 8323 MSRB III, Box 0646, Ann Arbor, MI 48109 0646, USA
    Infect Immun 73:7107-12. 2005
    ..These observations suggest that B. cenocepacia traverses polarized respiratory epithelium by the dephosphorylation and dissociation of occludin from the tight-junction complex...
  37. pmc Efficacy of bacteriophage therapy in a model of Burkholderia cenocepacia pulmonary infection
    Lisa A Carmody
    Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Infect Dis 201:264-71. 2010
    ..cenocepacia lung infection model. Systemic phage administration was more effective than inhalational administration, suggesting that circulating phages have better access to bacteria in lungs than do topical phages...
  38. doi request reprint Impact of burkholderia infection on lung transplantation in cystic fibrosis
    Susan Murray
    Department of Biostatistics, University of Michigan School of Public Health, Ann Arbor, Michigan 48109, USA
    Am J Respir Crit Care Med 178:363-71. 2008
    ..Infection with Burkholderia species is typically considered a contraindication to transplantation in CF. However, the risks posed by different Burkholderia species on transplantation outcomes are poorly defined...
  39. pmc Reassessment of Stenotrophomonas maltophilia phenotype
    Lisa A Carmody
    Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Clin Microbiol 49:1101-3. 2011
    ..Analysis of a collection of 766 S. maltophilia isolates indicated that approximately 20% are oxidase positive and that this species should be reevaluated for other phenotypes, including oxidative fermentation of lactose and sucrose...
  40. ncbi request reprint H. influenzae potentiates airway epithelial cell responses to rhinovirus by increasing ICAM-1 and TLR3 expression
    Umadevi S Sajjan
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, USA
    FASEB J 20:2121-3. 2006
    ..These data provide a cellular mechanism by which H. influenzae infection may increase the susceptibility of COPD patients to RV-induced exacerbations...
  41. ncbi request reprint Comparative proteomic analysis of B. cenocepacia using two-dimensional liquid separations coupled with mass spectrometry
    Kyu H Park
    Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109, USA
    Anal Chim Acta 592:91-100. 2007
    ..cenocepacia protein database. Many of the identified proteins in the clinical isolates are involved in gene translation and bacterial virulence such as transmissibility, resistance, and quorum sensing...
  42. ncbi request reprint Selection of nitrogen-fixing deficient Burkholderia vietnamiensis strains by cystic fibrosis patients: involvement of nif gene deletions and auxotrophic mutations
    Aymeric Menard
    Research Group on Bacterial Opportunistic Pathogens and Environment, Universite de Lyon, Lyon, F 69003, France
    Environ Microbiol 9:1176-85. 2007
    ..For 17% of B. vietnamiensis clinical strains, CF lungs appeared to have favoured the selection of mutations or deletions leading to N(2)-fixing deficiencies...
  43. pmc Environmental Burkholderia cepacia complex isolates in human infections
    Adam Baldwin
    Warwick University, Coventry, Wales, United Kingdom
    Emerg Infect Dis 13:458-61. 2007
    ..This finding links the natural environment with the emergence of many Bcc infections...
  44. doi request reprint Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex
    Elke Vanlaere
    Laboratorium voor Microbiologie, Universiteit Gent, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Int J Syst Evol Microbiol 58:1580-90. 2008
    ..nov. (type strain AU0553(T) =LMG 24068(T) =CCUG 54567(T)). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc...
  45. ncbi request reprint An outbreak of Burkholderia cepacia associated with contamination of albuterol and nasal spray
    Concepcion F Estivariz
    Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Chest 130:1346-53. 2006
    ..In March 2004, we investigated a hospital outbreak of Bcc possibly associated with a contaminated nasal spray...
  46. pmc Elucidating global epidemiology of Burkholderia multivorans in cases of cystic fibrosis by multilocus sequence typing
    Adam Baldwin
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Clin Microbiol 46:290-5. 2008
    ..multivorans...
  47. ncbi request reprint Multistate outbreak of Burkholderia cenocepacia colonization and infection associated with the use of intrinsically contaminated alcohol-free mouthwash
    Preeta K Kutty
    Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, MS A47, Atlanta, GA 30333, USA
    Chest 132:1825-31. 2007
    ..In 2005, we investigated a multistate outbreak of Burkholderia cenocepacia associated with an intrinsically contaminated alcohol-free mouthwash (AFM)...
  48. ncbi request reprint Risk factors for Burkholderia cepacia complex bacteremia among intensive care unit patients without cystic fibrosis: a case-control study
    Adam M Bressler
    Infectious Disease Specialists of Atlanta and Clinical Microbiology Laboratory, DeKalb Medical Center, Atlanta, GA, USA
    Infect Control Hosp Epidemiol 28:951-8. 2007
    ..The Burkholderia cepacia complex is associated with colonization or disease in patients with cystic fibrosis (CF). For patients without CF, this complex is poorly understood apart from its presence in occasional point source outbreaks...
  49. ncbi request reprint Identification of an RTX determinant of Burkholderia cenocepacia J2315 by subtractive hybridization
    Paul W Whitby
    Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Med Microbiol 55:11-21. 2006
    ....
  50. ncbi request reprint Virulence associated with outbreak-related strains of Burkholderia cepacia complex among a cohort of patients with bacteremia
    Christopher W Woods
    Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA
    Clin Infect Dis 38:1243-50. 2004
    ..5; 95% confidence interval, 1.20-25.02). B. cenocepacia is an emerging nosocomial pathogen. Certain strains are associated with an enhanced capacity for interpatient spread and poor outcome...
  51. ncbi request reprint Identification of genomic groups in the genus Stenotrophomonas using gyrB RFLP analysis
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, KL Ledeganckstraat 35, B 9000 Gent, Belgium
    FEMS Immunol Med Microbiol 40:181-5. 2004
    ..Our data also indicate that the majority of CF isolates grouped in two clusters. This may indicate that isolates from specific genomic groups have an increased potential for colonisation of the respiratory tract of CF patients...
  52. ncbi request reprint Outbreak of otitis media caused by Burkholderia gladioli infection in immunocompromised mice
    Patricia L Foley
    Center for Comparative Medicine, University of Virginia, Office of VP for Research and Graduate Studies, PO Box 800392, Charlottesville, Virginia, USA
    Comp Med 54:93-9. 2004
    ..The source and epidemiology of this organism remain unknown. This epizootic serves as an important reminder that immunocompromised rodent colonies may harbor important human opportunistic pathogens...
  53. pmc Updated version of the Burkholderia cepacia complex experimental strain panel
    Tom Coenye
    J Clin Microbiol 41:2797-8. 2003
  54. ncbi request reprint Burkholderia cenocepacia sp. nov.--a new twist to an old story
    Peter Vandamme
    Laboratory of Microbiology, University of Ghent, Ghent, Belgium
    Res Microbiol 154:91-6. 2003
    ..cepacia (genomovar I). A formal classification of B. cepacia genomovar III encompassing the recA lineages IIIA and IIIB, and the new recA lineages IIIC and IIID, as B. cenocepacia sp. nov., with LMG 16656 as the type strain, is proposed...
  55. ncbi request reprint Preventing Burkholderia cepacia complex infection in cystic fibrosis: is there a middle ground?
    John J LiPuma
    J Pediatr 141:467-9. 2002
  56. pmc Culture-based and non-growth-dependent detection of the Burkholderia cepacia complex in soil environments
    Suzanne C M Miller
    Department of Crop and Soil Science Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331 7306, USA
    Appl Environ Microbiol 68:3750-8. 2002
    ..Based on PCR detection, Bcc appears to be prevalent in soil from urban and suburban environments. Culture-based recovery of Bcc may underestimate environmental populations...
  57. ncbi request reprint Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools
    Peter Vandamme
    Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium
    FEMS Immunol Med Microbiol 33:143-9. 2002
    ..cepacia complex. This highlights another potential source for diagnostic problems with B. cepacia-like bacteria...
  58. pmc Six-year molecular analysis of Burkholderia cepacia complex isolates among cystic fibrosis patients at a referral center for lung transplantation
    David G Heath
    Clinical Microbiology Immunology Laboratories, University of North Carolina Hospitals, Chapel Hill 27514, USA
    J Clin Microbiol 40:1188-93. 2002
    ..These data suggest that CF patients infected with B. cepacia complex and referred for lung transplantation evaluation were not a major source of B. cepacia complex strains that infected our resident CF clinic population...
  59. ncbi request reprint Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov
    Karen Vermis
    Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Int J Syst Evol Microbiol 54:689-91. 2004
    ..cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification...
  60. pmc Involvement of a plasmid-encoded type IV secretion system in the plant tissue watersoaking phenotype of Burkholderia cenocepacia
    Amanda S Engledow
    Department of Plant Pathology and Microbiology, Texas A and M University, 2132 TAMU, College Station, TX 77843, USA
    J Bacteriol 186:6015-24. 2004
    ..Our findings indicate that in strain K56-2, the plasmid-encoded Ptw type IV secretion system is responsible for the secretion of a plant cytotoxic protein(s)...
  61. pmc Divergence and mosaicism among virulent soil phages of the Burkholderia cepacia complex
    Elizabeth J Summer
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    J Bacteriol 188:255-68. 2006
    ..However, the Bcep781 group phages can also engage in lateral gene transfer events with otherwise unrelated phages, a process that contributes to the broader-scale genomic mosaicism prevalent among the tailed phages...
  62. pmc Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex
    Adam Baldwin
    Department of Biological Sciences, University of Warwick, Coventry, UK
    J Clin Microbiol 43:4665-73. 2005
    ..It is also the first MLST scheme designed at the outset to incorporate multiple species and should facilitate global epidemiological investigations of the BCC...
  63. pmc Development of a recA gene-based identification approach for the entire Burkholderia genus
    George W Payne
    Cardiff School of Biosciences, Main Building, Museum Avenue, PO Box 915, Cardiff University, Cardiff, Wales CF10 3TL, United Kingdom
    Appl Environ Microbiol 71:3917-27. 2005
    ..cepacia complex strains recovered from clinical infection versus the natural environment, it did facilitate the identification of clonal strain types of B. cepacia, B. stabilis, and B. ambifaria capable of residing in both niches...
  64. pmc Use of PCR analyses to define the distribution of Ralstonia species recovered from patients with cystic fibrosis
    Tom Coenye
    Laboratory for Microbiology, Faculty of Sciences, University of Ghent, Ghent, Belgium
    J Clin Microbiol 43:3463-6. 2005
    ..respiraculi and Ralstonia pickettii accounted for 19% and 18%, respectively. Ralstonia basilensis and Ralstonia metallidurans, species not previously recovered from human samples, were also identified...
  65. ncbi request reprint Identification of DNA markers for a transmissible Pseudomonas aeruginosa cystic fibrosis strain
    Deborah A Lewis
    Cardiff School of Biosciences, Cardiff University, UK
    Am J Respir Cell Mol Biol 33:56-64. 2005
    ..The MA genomic island and Vibrio cholera-like toxin gene within it constitute novel genetic factors associated with a transmissible P. aeruginosa strain and their role in pathogenesis remains to be determined...
  66. pmc Species abundance and diversity of Burkholderia cepacia complex in the environment
    Alban Ramette
    Center for Microbial Ecology, 540 Plant and Soil Sciences Building, Michigan State University, East Lansing, MI 48824 1325, USA
    Appl Environ Microbiol 71:1193-201. 2005
    ..cepacia complex species and closely related strains of the same species may coexist at high population levels but also species composition and abundance may dramatically vary between individual plants...
  67. ncbi request reprint Polyphasic characterisation of Burkholderia cepacia-like isolates leading to the emended description of Burkholderia pyrrocinia
    Virginie Storms
    Laboratory for Microbiology, Faculty of Sciences, University of Ghent, Ghent, Belgium
    Syst Appl Microbiol 27:517-26. 2004
    ..cepacia complex species thus far comprising one single soil isolate only. An emended description of Burkholderia pyrrocinia is proposed. The taxonomic status of the remaining two isolates requires further analysis...
  68. pmc Correlation of wbiI genotype, serotype, and isolate source within species of the Burkholderia cepacia complex
    Arlene D Vinion-Dubiel
    Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, VA, USA
    J Clin Microbiol 42:4121-6. 2004
    ..However three of five Bcc outbreaks involved isolates with the same wbiI RFLP type, indicating that wbiI RFLP typing may be a useful tool to help track Bcc outbreaks...
  69. pmc Sepsis, multiple organ failure, and death due to Pandoraea pnomenusa infection after lung transplantation
    Martin E Stryjewski
    Division of Infectious Diseases and International Health, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, USA
    J Clin Microbiol 41:2255-7. 2003
    ..Heretofore considered soil bacteria and colonizers that infect patients with chronic lung diseases, Pandoraea species can produce severe infections...
  70. ncbi request reprint In vitro activity and synergy of bismuth thiols and tobramycin against Burkholderia cepacia complex
    Wilfredo G Veloira
    Department of Pediatrics Pulmonary Medicine and Neonatology, CardioPulmonary Research Institute, Winthrop University Hospital, SUNY Stony Brook School of Medicine, Mineola, NY, USA
    J Antimicrob Chemother 52:915-9. 2003
    ..To determine the susceptibility of Burkholderia multivorans and Burkholderia cenocepacia to bismuth-thiols (BTs), and to examine the synergistic effects of tobramycin and subinhibitory concentrations of BTs against these organisms...
  71. ncbi request reprint Lack of cable pili expression by cblA-containing Burkholderia cepacia complex
    Umadevi Sajjan
    Department of Structural Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Microbiology 148:3477-84. 2002
    ....
  72. ncbi request reprint Impact of Burkholderia dolosa on lung function and survival in cystic fibrosis
    Leslie A Kalish
    Infectious Diseases Division, Clinical Research Program Division of Respiratory Diseases, Infection Control Program, Department of Laboratory Medicine, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    Am J Respir Crit Care Med 173:421-5. 2006
    ..Clinical implications of the recently characterized genomovar VI, B. dolosa, are unknown...
  73. pmc Involvement of a quorum-sensing-regulated lipase secreted by a clinical isolate of Burkholderia glumae in severe disease symptoms in rice
    Giulia Devescovi
    Bacteriology Group, International Centre for Genetic Engineering and Biotechnology, Padriciano 99, Trieste, Italy
    Appl Environ Microbiol 73:4950-8. 2007
    ..It is the first report of a clinical B. glumae isolate retaining strong rice pathogenicity and finally determined that B. glumae can undergo phenotypic conversion through a spontaneous mutation in the tofR regulator...

Research Grants2

  1. Burkholderia sp: Identification of major clonal lineages
    John LiPuma; Fiscal Year: 2004
    ..mallei and B. pseudomallei. ..