Affiliation: University of California
Qin W, Lu X, Liu Y, Bai H, Li S, Lin S. Precise A•T to G•C base editing in the zebrafish genome. BMC Biol. 2018;16:139 pubmed publisher
..Collectively, this system, designated as zABE7.10, provides a strategy to perform A•T to G•C base editing in zebrafish and enhances its capacity to model human diseases. ..
Qin W, Lu X, Lin S. Programmable base editing in zebrafish using a modified CRISPR-Cas9 system. Methods. 2018;150:19-23 pubmed publisher
..Here we describe a protocol for using the base editing system in zebrafish and its application to reproduce a single base mutation observed in human Ablepharon-Macrostomia Syndrome. ..
Veldman M, Lin S. Stem cells on fire: inflammatory signaling in HSC emergence. Dev Cell. 2014;31:517-8 pubmed publisher
..Recent reports demonstrate the surprising involvement of these pathways during hematopoietic stem cell emergence from the hemogenic endothelium in both zebrafish and mice. ..
Danilova N, Wilkes M, Bibikova E, Youn M, Sakamoto K, Lin S. Innate immune system activation in zebrafish and cellular models of Diamond Blackfan Anemia. Sci Rep. 2018;8:5165 pubmed publisher
..Inhibitors of complement and activin also had beneficial effects. Our studies suggest that innate immune system contributes to the phenotype of RPS19-deficient zebrafish and human cells. ..
Huang H, Zhang B, Hartenstein P, Chen J, Lin S. NXT2 is required for embryonic heart development in zebrafish. BMC Dev Biol. 2005;5:7 pubmed
..The defects could be reproduced by morpholino anti-sense oligo knockdown of NXT2. NXT2 has a critical role in maintaining morphogenetic integrity of embryonic heart in vertebrate species. ..
Yang Z, Jiang H, Zhao F, Shankar D, Sakamoto K, Zhang M, et al
. A highly conserved regulatory element controls hematopoietic expression of GATA-2 in zebrafish. BMC Dev Biol. 2007;7:97 pubmed
..The integrated approach described here, drawing on embryological, transgenesis and computational methods, should be generally applicable to analyze tissue-specific gene regulation involving distal DNA cis-acting elements. ..
Kim H, Schleiffarth J, Jessurun J, Sumanas S, Petryk A, Lin S, et al
. Wnt5 signaling in vertebrate pancreas development. BMC Biol. 2005;3:23 pubmed
..This study opens the door for further investigation into a role of Wnt signaling in vertebrate organ development and disease. ..
Veldman M, Rios Galdamez Y, Lu X, Gu X, Qin W, Li S, et al
. The N17 domain mitigates nuclear toxicity in a novel zebrafish Huntington's disease model. Mol Neurodegener. 2015;10:67 pubmed publisher
..Future experiments will be focused on identifying genetic modifiers, mechanisms and therapeutics that alleviate polyQ aggregation in the nucleus of neurons. ..
Ear J, Hsueh J, Nguyen M, Zhang Q, Sung V, Chopra R, et al
. A Zebrafish Model of 5q-Syndrome Using CRISPR/Cas9 Targeting RPS14 Reveals a p53-Independent and p53-Dependent Mechanism of Erythroid Failure. J Genet Genomics. 2016;43:307-18 pubmed publisher
..Finally, we demonstrate the versatility of this model to test various pharmacological agents, such as RAP-011, L-leucine, and dexamethasone in order to identify molecules that can reverse the anemic phenotype. ..