Ching Shwun Lin

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Tracking intracavernously injected adipose-derived stem cells to bone marrow
    G Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Int J Impot Res 23:268-75. 2011
  2. ncbi request reprint Stem-cell therapy for erectile dysfunction
    Ching Shwun Lin
    University of California, School of Medicine, Department of Urology, Knuppe Molecular Urology Laboratory, San Francisco, CA 94143 0738, USA 1 415 476 3800 1 415 476 3803
    Expert Opin Biol Ther 13:1585-97. 2013
  3. pmc Commonly used mesenchymal stem cell markers and tracking labels: Limitations and challenges
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Histol Histopathol 28:1109-16. 2013
  4. pmc Is CD34 truly a negative marker for mesenchymal stromal cells?
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Cytotherapy 14:1159-63. 2012
  5. doi request reprint Direct androgen regulation of PDE5 gene or the lack thereof
    C S Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, San Francisco, CA94143 0738, USA
    Int J Impot Res 25:81-5. 2013
  6. pmc Defining vascular stem cells
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 22:1018-26. 2013
  7. pmc Phosphodiesterase-5 expression and function in the lower urinary tract: a critical review
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Urology 81:480-7. 2013
  8. pmc Allogeneic and xenogeneic transplantation of adipose-derived stem cells in immunocompetent recipients without immunosuppressants
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 21:2770-8. 2012
  9. pmc Stem cell therapy for stress urinary incontinence: a critical review
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 21:834-43. 2012
  10. pmc Stem cell therapy for erectile dysfunction: a critical review
    Ching Shwun Lin
    Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 21:343-51. 2012

Collaborators

Detail Information

Publications80

  1. pmc Tracking intracavernously injected adipose-derived stem cells to bone marrow
    G Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Int J Impot Res 23:268-75. 2011
    ..The significance of these results in relation to SC therapy for ED is discussed...
  2. ncbi request reprint Stem-cell therapy for erectile dysfunction
    Ching Shwun Lin
    University of California, School of Medicine, Department of Urology, Knuppe Molecular Urology Laboratory, San Francisco, CA 94143 0738, USA 1 415 476 3800 1 415 476 3803
    Expert Opin Biol Ther 13:1585-97. 2013
    ..Stem cells (SCs) have been investigated for the treatment of erectile dysfunction (ED)...
  3. pmc Commonly used mesenchymal stem cell markers and tracking labels: Limitations and challenges
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Histol Histopathol 28:1109-16. 2013
    ..These caveats need to be taken into consideration when designing or interpreting MSC transplantation experiments. ..
  4. pmc Is CD34 truly a negative marker for mesenchymal stromal cells?
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Cytotherapy 14:1159-63. 2012
    ..Thus the available evidence points to CD34 being expressed in tissue-resident MSC, and its negative finding being a consequence of cell culturing...
  5. doi request reprint Direct androgen regulation of PDE5 gene or the lack thereof
    C S Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, San Francisco, CA94143 0738, USA
    Int J Impot Res 25:81-5. 2013
    ..Thus, it appears that reduced PDE5 expression in castrated animals is due to reduced smooth muscle content and that PDE5A gene is not directly regulated by androgens...
  6. pmc Defining vascular stem cells
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 22:1018-26. 2013
    ..These proposed in vivo functions are areas of interest for future research on VSCs...
  7. pmc Phosphodiesterase-5 expression and function in the lower urinary tract: a critical review
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Urology 81:480-7. 2013
    ..For PDE-5 expression, the data have ranged from extremely low to highly abundant. PDE-5 has been found in the glandular epithelium, vascular smooth muscle, endothelium, and fibromuscular stroma...
  8. pmc Allogeneic and xenogeneic transplantation of adipose-derived stem cells in immunocompetent recipients without immunosuppressants
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 21:2770-8. 2012
    ..But in veterinary medicine, xenotransplantation with porcine ADSC is a practical alternative to the costly and inconvenient autotransplantation...
  9. pmc Stem cell therapy for stress urinary incontinence: a critical review
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 21:834-43. 2012
    ..In any event, SC transplantation appears to be a promising treatment for SUI...
  10. pmc Stem cell therapy for erectile dysfunction: a critical review
    Ching Shwun Lin
    Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 21:343-51. 2012
    ..This could perhaps explain why intracavernously injected SCs had systemic antidiabetic effects and prolonged anti-ED effects. These hypotheses and the differentiation-versus-paracrine debate require further investigation...
  11. ncbi request reprint Defining adipose tissue-derived stem cells in tissue and in culture
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA, USA
    Histol Histopathol 25:807-15. 2010
    ..In the larger vessels, these ADSC or VSC exist as specialized fibroblasts (having stem cell properties) in the adventitia...
  12. pmc Molecular Yin and Yang of erectile function and dysfunction
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, University of California, San Francisco, CA 94143, USA
    Asian J Androl 10:433-40. 2008
    ..g., Rho kinase and protein kinase G), branching into secondary effectors, and finishing with contractile molecules and phosphodiesterases. Interactions between the Yin and Yang signaling pathways are discussed as well...
  13. doi request reprint Recent advances in andrology-related stem cell research
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California San Francisco, CA 94143, USA
    Asian J Androl 10:171-5. 2008
    ..We are also investigating whether ADSC can differentiate into Leydig, Sertoli and male germ cells. The eventual goal is to use ADSC to treat male infertility and testosterone deficiency...
  14. pmc Pentoxifylline attenuates transforming growth factor-beta1-stimulated elastogenesis in human tunica albuginea-derived fibroblasts part 2: Interference in a TGF-beta1/Smad-dependent mechanism and downregulation of AAT1
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA 94143 0738, United States
    J Sex Med 7:1787-97. 2010
    ..Pentoxifylline (PTX) antagonizes the effects of TGF-beta1 and has been utilized in our clinic for the management of PD although the mechanisms of action are not entirely clear...
  15. pmc Treatment of stress urinary incontinence with adipose tissue-derived stem cells
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California, USA
    Cytotherapy 12:88-95. 2010
    ..Effective treatment for stress urinary incontinence (SUI) is lacking. This study investigated whether transplantation of adipose tissue-derived stem cells (ADSC) can treat SUI in a rat model...
  16. pmc Recruitment of intracavernously injected adipose-derived stem cells to the major pelvic ganglion improves erectile function in a rat model of cavernous nerve injury
    Thomas M Fandel
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA, USA
    Eur Urol 61:201-10. 2012
    ..Intracavernous (IC) injection of stem cells has been shown to ameliorate cavernous-nerve (CN) injury-induced erectile dysfunction (ED). However, the mechanisms of action of adipose-derived stem cells (ADSC) remain unclear...
  17. pmc Pentoxifylline promotes recovery of erectile function in a rat model of postprostatectomy erectile dysfunction
    Maarten Albersen
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Eur Urol 59:286-96. 2011
    ..Pentoxifylline (PTX) is a phosphodiesterase inhibitor that further inhibits multiple cytokine pathways involved in nerve degeneration, apoptosis, and fibrosis...
  18. doi request reprint Identification of active and quiescent adipose vascular stromal cells
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Cytotherapy 14:240-6. 2012
    ..Recent studies have demonstrated the existence of both active and quiescent stem cells in bone marrow, hair follicle and intestine. We attempted to identify active and quiescent vascular stromal cells (VSC) in adipose tissue...
  19. pmc Treatment of erectile dysfunction in the obese type 2 diabetic ZDF rat with adipose tissue-derived stem cells
    Maurice M Garcia
    Department of Urology, University of California School of Medicine Knuppe Molecular Urology Laboratory, San Francisco, CA 94143 0738, USA
    J Sex Med 7:89-98. 2010
    ..Erectile dysfunction (ED) is a major complication of type 2 diabetes, and many diabetic men with ED are refractory to common ED therapies...
  20. pmc The effect of intracavernous injection of adipose tissue-derived stem cells on hyperlipidemia-associated erectile dysfunction in a rat model
    Yun Ching Huang
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA, USA
    J Sex Med 7:1391-400. 2010
    ..Adipose tissue-derived stem cells (ADSC) have been shown to differentiate into endothelial cells and secrete vasculotrophic and neurotrophic factors...
  21. pmc Erectogenic and neurotrophic effects of icariin, a purified extract of horny goat weed (Epimedium spp.) in vitro and in vivo
    Alan W Shindel
    University of California Knuppe Molecular Urology Laboratory Department of Urology, School of Medicine, San Francisco, CA, USA
    J Sex Med 7:1518-28. 2010
    ..Epimedium species (aka horny goat weed) have been utilized for the treatment of erectile dysfunction in Traditional Chinese Medicine for many years. Icariin (ICA) is the active moiety of Epimedium species...
  22. pmc Both immediate and delayed intracavernous injection of autologous adipose-derived stromal vascular fraction enhances recovery of erectile function in a rat model of cavernous nerve injury
    Xuefeng Qiu
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA 94143 0738, USA
    Eur Urol 62:720-7. 2012
    ..However, culturing exposes ADSCs to the risk of contamination and dedifferentiation...
  23. pmc MicroRNA regulation of neuron-like differentiation of adipose tissue-derived stem cells
    Hongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Differentiation 78:253-9. 2009
    ..Thus, IGF-I signaling and miR-133b co-regulate potential neural differentiation of ADSC through a feedback mechanism, in which IGF-I upregulates miR-133b while miR-133b in turn downregulates IGF-IR...
  24. pmc Effects of prolonged vaginal distension and β-aminopropionitrile on urinary continence and urethral structure
    Guifang Wang
    Department of Urology, University of California, School of Medicine, San Francisco, CA, USA
    Urology 78:968.e13-9. 2011
    ..To investigate the effects of prolonged vaginal distension and β-aminopropionitrile (BAPN) on the urinary patterns and urethral structure in female virgin rats...
  25. pmc Labeling and tracking of mesenchymal stromal cells with EdU
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Cytotherapy 11:864-73. 2009
    ..In the present study we tested a new thymidine analog, 5-ethynyl-2-deoxyuridine (EdU), for labeling and tracking of mesenchymal stromal cells (MSC), specifically adipose tissue-derived stem cells (ADSC)...
  26. pmc Effects of birth trauma and estrogen on urethral elastic fibers and elastin expression
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California San Francisco, San Francisco, California, USA
    Urology 76:1018.e8-13. 2010
    ..To investigate the effects of birth trauma and estrogen on urethral elastic fibers and elastin expression...
  27. ncbi request reprint Up and down-regulation of phosphodiesterase-5 as related to tachyphylaxis and priapism
    Guiting Lin
    Department of Urology, Knuppe Molecular Urology Laboratory, University of California School of Medicine, San Francisco, CA 94143 1695, USA
    J Urol 170:S15-8; discussion S19. 2003
    ..We identify whether tachyphylaxis and priapism effects of sildenafil are related to regulation of phosphodiesterase-5 (PDE-5) expression...
  28. pmc Effects of transplantation of adipose tissue-derived stem cells on prostate tumor
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Prostate 70:1066-73. 2010
    ..Obesity is a risk factor for prostate cancer development, but the underlying mechanism is unknown. The present study tested the hypothesis that stromal cells of the adipose tissue might be recruited by cancer cells to help tumor growth...
  29. pmc Effects of low-energy shockwave therapy on the erectile function and tissue of a diabetic rat model
    Xuefeng Qiu
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    J Sex Med 10:738-46. 2013
    ..Qiu X, Lin G, Xin Z, Ferretti L, Zhang H, Lue TF, and Lin C-S. Effects of low-energy shockwave therapy on the erectile function and tissue of a diabetic rat model. J Sex Med 2013;10:738-746...
  30. ncbi request reprint The effect of vascular endothelial growth factor on a rat model of traumatic arteriogenic erectile dysfunction
    Ming Chan Lee
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California School of Medicine, San Francisco, California
    J Urol 167:761-7. 2002
    ..We tested the hypothesis that intracavernous injection of vascular endothelial growth factor (VEGF) can restore erectile function in a rat model of traumatic arteriogenic erectile dysfunction...
  31. ncbi request reprint Improving erectile function by silencing phosphodiesterase-5
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California San Francisco 94143 1695, USA
    J Urol 174:1142-8. 2005
    ..We investigated whether phosphodiesterase-5 (PDE5) can be down-regulated by a specific small interfering RNA (siRNA) and whether this would improve erectile function...
  32. pmc Fibroblast growth factor 2 promotes endothelial differentiation of adipose tissue-derived stem cells
    Hongxiu Ning
    University of California, School of Medicine, Knuppe Molecular Urology Laboratory Department of Urology, San Francisco, California 94143, USA
    J Sex Med 6:967-79. 2009
    ..Adipose tissue-derived stem cells (ADSC) could potentially restore endothelial function in vasculogenic erectile dysfunction (ED). The mechanism for ADSC endothelial differentiation remained unidentified...
  33. pmc Tissue distribution of mesenchymal stem cell marker Stro-1
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 20:1747-52. 2011
    ..These variable and endothelial expression patterns of Stro-1 point to a need to re-examine published data that relied on Stro-1 as a mesenchymal stem cell marker...
  34. ncbi request reprint Neuron-like differentiation of adipose tissue-derived stromal cells and vascular smooth muscle cells
    Hongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 1695, USA
    Differentiation 74:510-8. 2006
    ..Finally, we showed that vascular smooth muscle cells could also be induced by either NIM or IBMX to differentiate into neuron-like cells that expressed NF70...
  35. doi request reprint Effects of estrogen, raloxifene and levormeloxifene on alpha1A-adrenergic receptor expression
    Lia Banie
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California San Francisco, San Francisco, California 94143 0738, USA
    J Urol 180:2241-6. 2008
    ..We investigated the effect of estrogen, raloxifene and levormeloxifene on alpha1A-adrenergic receptor expression...
  36. pmc Bone marrow cells stained by azide-conjugated Alexa fluors in the absence of an alkyne label
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 21:2552-9. 2012
    ..Hematopoietic and mesenchymal stem cell markers CD34 and Stro-1 did not co-localize with the false-positively stained cells, and these cells' identity remains unknown...
  37. pmc Label retaining and stem cell marker expression in the developing rat urinary bladder
    Haiyang Zhang
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Urology 79:746.e1-6. 2012
    ..To identify potential stem cells in urinary bladder by label-retaining cell (LRC) strategy and immunostaining for putative stem cell markers...
  38. pmc Cavernous nerve repair with allogenic adipose matrix and autologous adipose-derived stem cells
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA 94143 0738, USA
    Urology 77:1509.e1-8. 2011
    ..To investigate whether adipose-derived matrix seeded with adipose-derived stem cells (ADSC) can facilitate the repair of injured cavernous nerves (CNs)...
  39. pmc Pentoxifylline attenuates transforming growth factor-β1-stimulated collagen deposition and elastogenesis in human tunica albuginea-derived fibroblasts part 1: impact on extracellular matrix
    Alan W Shindel
    University of California Knuppe Molecular Urology Laboratory, Department of Urology, San Francisco, CA 94143, USA
    J Sex Med 7:2077-85. 2010
    ..Pentoxifylline (PTX) antagonizes the effects of TGF-β1 and has been utilized in our clinic for the management of PD...
  40. pmc The effect of long-term hormonal treatment on voiding patterns during filling cystometry and on urethral histology in a postpartum, ovariectomized female rat
    Benjamin N Breyer
    Department of Urology, Knuppe Molecular Urology Laboratory, University of California, San Francisco, CA, USA
    BJU Int 106:1775-81. 2010
    ....
  41. pmc Effects of EdU labeling on mesenchymal stem cells
    Hongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143, USA
    Cytotherapy 15:57-63. 2013
    ..In the present study, we tested whether EdU was cytotoxic and whether it interfered with differentiation, cytokine secretion and migration of MSCs...
  42. ncbi request reprint Effects of icariin on phosphodiesterase-5 activity in vitro and cyclic guanosine monophosphate level in cavernous smooth muscle cells
    Hongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, School of Medicine, San Francisco, California 94115, USA
    Urology 68:1350-4. 2006
    ..To investigate the effect of icariin on the cyclic guanosine monophosphate (cGMP)-hydrolytic activity of phosphodiesterase-5 (PDE5) isoforms and the cGMP levels in cavernous smooth muscle cells treated with sodium nitroprusside (SNP)...
  43. ncbi request reprint Long-term effect of ovariectomy and simulated birth trauma on the lower urinary tract of female rats
    Julio Resplande
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California San Francisco, San Francisco, California, USA
    J Urol 168:323-30. 2002
    ..Using an animal model we studied the long-term effects of ovariectomy and simulated birth trauma in the development of apoptosis as well as the urodynamic, histological and ultrastructural findings 9 months after such procedures...
  44. pmc Injections of adipose tissue-derived stem cells and stem cell lysate improve recovery of erectile function in a rat model of cavernous nerve injury
    Maarten Albersen
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    J Sex Med 7:3331-40. 2010
    ..However, the mechanisms of action for stem cell therapy remain controversial, with increasing evidence pointing to paracrine pathways...
  45. pmc Effects of intravenous injection of adipose-derived stem cells in a rat model of radiation therapy-induced erectile dysfunction
    Xuefeng Qiu
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    J Sex Med 9:1834-41. 2012
    ..Radiation therapy (RT) for prostate cancer is frequently associated with posttreatment erectile dysfunction (ED)...
  46. pmc Neurotrophic effects of brain-derived neurotrophic factor and vascular endothelial growth factor in major pelvic ganglia of young and aged rats
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA, USA
    BJU Int 105:114-20. 2010
    ..To investigate the neurotrophic effect of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) in cultured major pelvic ganglia (MPG) derived from young and aged rats...
  47. pmc Adipose tissue-derived stem cells secrete CXCL5 cytokine with neurotrophic effects on cavernous nerve regeneration
    Haiyang Zhang
    Knuppe Molecular Urology Laboratory Department of Urology, School of Medicine, University of California, San Francisco, CA 94143, USA
    J Sex Med 8:437-46. 2011
    ..Previously we reported that paracrine actions likely mediated the therapeutic effects of adipose tissue-derived stem cells (ADSCs) on a rat model of cavernous nerve (CN) injury...
  48. doi request reprint Effects of high glucose on human cavernous endothelial cells
    Hongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, School of Medicine, San Francisco, CA 94143 0738, USA
    Urology 80:1162.e7-11. 2012
    ..To obtain experimental evidence for a causal effect of high glucose (HG) on cavernous endothelial dysfunction...
  49. doi request reprint Transdifferentiation of adipose-derived stem cells into hepatocytes: a new approach
    James Lue
    Department of Pediatrics, Division of Pediatric Gastroenterology, Hepatology, and Nutrition, Stanford University, Palo Alto, CA, USA
    Liver Int 30:913-22. 2010
    ..Unfortunately, transdifferentiation is inefficient, and the function of these induced hepatocyte-like cells (which we termed 'iHeps') is low compared with that of real hepatocytes...
  50. pmc Adipose tissue-derived stem cells secrete CXCL5 cytokine with chemoattractant and angiogenic properties
    Haiyang Zhang
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Biochem Biophys Res Commun 402:560-4. 2010
    ..Migration assay indicates chemoattractant effects of CXCL5 on ADSC and HUVEC endothelial cells. CXCL5 also enhanced matrigel-based endothelial tube-like formation of HUVEC...
  51. pmc Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes
    Guiting Lin
    Department of Urology, University of California, San Francisco, CA, USA
    Urology 78:970.e1-8. 2011
    ..To investigate whether fluorochrome-conjugated phalloidin can delineate cavernous smooth muscle (CSM) cells and whether it can be combined with immunofluorescence (IF) staining to quantify erectile dysfunction (ED)-associated changes...
  52. pmc Modulation of smooth muscle tonus in the lower urinary tract: interplay of myosin light-chain kinase (MLCK) and MLC phosphatase (MLCP)
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California School of Medicine, San Francisco, CA 94143 0738, USA
    BJU Int 108:E66-70. 2011
    ..To assess and compare the expression and activity of myosin light-chain kinase (MLCK) and MLC phosphatase (MLCP) in rat bladder and urethra...
  53. doi request reprint Intracavernous growth differentiation factor-5 therapy enhances the recovery of erectile function in a rat model of cavernous nerve injury
    Thomas M Fandel
    University of California, San Francisco Urology, San Francisco, CA 94143 0738, USA
    J Sex Med 5:1866-75. 2008
    ..Neurogenic erectile dysfunction remains a serious complication in the postprostatectomy population. Effective protective and regenerative neuromodulatory strategies are needed...
  54. pmc Adipose derived stem cells ameliorate hyperlipidemia associated detrusor overactivity in a rat model
    Yun Ching Huang
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California San Francisco, San Francisco, California 94143 0738, USA
    J Urol 183:1232-40. 2010
    ..Adipose tissue derived stem cells can differentiate into muscle and neuron-like cells in vitro. We investigate the usefulness of adipose tissue derived stem cells for overactive bladder in obese hyperlipidemic rats...
  55. ncbi request reprint Tunica albuginea allograft: a new model of LaPeyronie's disease with penile curvature and subtunical ossification
    Ludovic Ferretti
    Department of Urology, University of Bordeaux, Bordeaux, France Department of Urology, Knuppe Molecular Urology Laboratory, University of California San Francisco, San Francisco, California, USA Hospital d Instruction des Armées Robert Picqué, Villenave D Ornon, France
    Asian J Androl 16:592-6. 2014
    ..Validation studies using this animal model would aid understanding of the PD pathophysiology for effective therapeutic interventions. ..
  56. pmc Defining stem and progenitor cells within adipose tissue
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 17:1053-63. 2008
    ..Isolated, ADSC can be induced to differentiate into additional cell types such as osteoblasts and chondrocytes...
  57. pmc Potential of adipose-derived stem cells for treatment of erectile dysfunction
    Guiting Lin
    School of Medicine, Department of Urology, University of California Knuppe Molecular Urology Laboratory, San Francisco, CA 94143 0738, USA
    J Sex Med 6:320-7. 2009
    ....
  58. ncbi request reprint Phosphodiesterase-5 isoforms: differential cyclic guanyl monophosphate binding and cyclic guanyl monophosphate catalytic activities, and inhibitory effects of sildenafil and vardenafil
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California San Francisco, CA 94143, USA
    J Urol 176:1242-7. 2006
    ....
  59. pmc Treatment of type 1 diabetes with adipose tissue-derived stem cells expressing pancreatic duodenal homeobox 1
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143 0738, USA
    Stem Cells Dev 18:1399-406. 2009
    ..Histological examination showed that the transplanted cells formed tissue-like structures and expressed insulin. Thus, ADSC-expressing Pdx1 appear to be suitable for treatment of DM...
  60. pmc Effects of estrogen, raloxifene, and levormeloxifene on the expression of Rho-kinase signaling molecules in urethral smooth muscle cells
    Nongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143, USA
    Urology 76:1517.e6-11. 2010
    ..To investigate the effects of estrogen, raloxifene, and levormeloxifene on the expression of Rho-kinase signaling molecules in urethral smooth muscle cells (USMCs)...
  61. ncbi request reprint Estradiol upregulates activating transcription factor 3, a candidate gene in the etiology of hypospadias
    Benchun Liu
    Department of Urology, University of California, San Francisco, CA, USA
    Pediatr Dev Pathol 10:446-54. 2007
    ..Its potential involvement in other manifestations of developmental endocrine disruption is worth investigating...
  62. ncbi request reprint Upregulation of penile brain-derived neurotrophic factor (BDNF) and activation of the JAK/STAT signalling pathway in the major pelvic ganglion of the rat after cavernous nerve transection
    Anthony J Bella
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA 94143 0738, USA
    Eur Urol 52:574-80. 2007
    ....
  63. ncbi request reprint Cyclic nucleotide signaling in cavernous smooth muscle
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA 94143 1695, USA
    J Sex Med 2:478-91. 2005
    ..The involvement of these molecules in the development of erectile dysfunction and the possibility of using them as therapeutic agents or targets are also discussed...
  64. ncbi request reprint Phosphodiesterases as therapeutic targets
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, School of Medicine, 94115, USA
    Urology 61:685-91. 2003
  65. pmc Insulin growth factor signaling mediates neuron-like differentiation of adipose-tissue-derived stem cells
    Hongxiu Ning
    Knuppe Molecular Urology Laboratory Department of Urology, School of Medicine, University of California San Francisco, CA 94143 0738, USA
    Differentiation 76:488-94. 2008
    ..This effect was also abrogated by PPP. Thus, the IBMX-induced neuron-like differentiation of ADSC is mediated by IGF signaling through the phosphorylation of IGF-IR at Y1136...
  66. ncbi request reprint Brain-derived neurotrophic factor (BDNF) acts primarily via the JAK/STAT pathway to promote neurite growth in the major pelvic ganglion of the rat: part 2
    Guiting Lin
    Knuppe Molecular Urology, Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 1695, USA
    J Sex Med 3:821-7; discussion 828-9. 2006
    ..We previously showed that brain-derived neurotrophic factor (BDNF) could restore erectile function in a neurogenic ED rat model. We now investigated the signaling mechanism of BDNF in major pelvic ganglia (MPG) explants...
  67. pmc Cavernous smooth muscle hyperplasia in a rat model of hyperlipidaemia-associated erectile dysfunction
    Xuefeng Qiu
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, USA
    BJU Int 108:1866-72. 2011
    ..This study shows that the increased smooth muscle content is due to hyperplasia...
  68. pmc Prominent expression of phosphodiesterase 5 in striated muscle of the rat urethra and levator ani
    Guiting Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California San Francisco, California 94143 0738, USA
    J Urol 184:769-74. 2010
    ..We investigated phosphodiesterase 5 distribution and activity in the urethra...
  69. pmc Lack of direct androgen regulation of PDE5 expression
    Rong Yang
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, 533 Parnassus Ave, Box 0738, San Francisco, CA 94143 0738, USA
    Biochem Biophys Res Commun 380:758-62. 2009
    ..Prostate specific antigen (PSA) promoter activity was upregulated while PDE5A promoter activity remained unchanged in DHT-treated CSMC. Thus, PDE5 expression was not under direct androgen regulation...
  70. pmc Identification of an aberrant cell line among human adipose tissue-derived stem cell isolates
    Hongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Differentiation 77:172-80. 2009
    ..The results showed that the established angiosarcoma cell lines exhibit considerable variations among themselves and HADSC-6 displayed most of these variable characteristics...
  71. ncbi request reprint Expression, distribution and regulation of phosphodiesterase 5
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, 1657 Scott Street, Room 210, Box 1695, San Francisco, CA 94115, USA
    Curr Pharm Des 12:3439-57. 2006
    ..Virtually all tissues and cell types express PDE5, with heart and cardiomyocytes being contentious. PDE5A1 and PDE5A2 are ubiquitous, but PDE5A3 is specific to smooth muscle...
  72. doi request reprint Scaffoldless tissue engineering of stem cell derived cavernous tissue for treatment of erectile function
    Hazem Orabi
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA, USA
    J Sex Med 9:1522-34. 2012
    ..Adipose tissue-derived stem cells (ADSCs) represent an ideal source for new ED treatment...
  73. pmc Mesenchymal stem cell marker Stro-1 is a 75 kd endothelial antigen
    Hongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 0738, USA
    Biochem Biophys Res Commun 413:353-7. 2011
    ..These data, together with previous studies, indicate that Stro-1 is intrinsically an endothelial antigen, and its expression in MSC is probably an induced event...
  74. pmc Functional, metabolic, and morphologic characteristics of a novel rat model of type 2 diabetes-associated erectile dysfunction
    Maarten Albersen
    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA, USA
    Urology 78:476.e1-8. 2011
    ..To conduct a pilot study to investigate functional, metabolic, and penile morphologic changes in a novel model of lean DM2. Erectile dysfunction (ED) is a frequent sequela in patients with type 2 diabetes mellitus (DM2)...
  75. ncbi request reprint [The in vitro and in vivo experimental models of erectile nerve regeneration]
    Gui Ting Lin
    Department of Urology, University of California, San Francisco, CA 94143, USA
    Zhonghua Nan Ke Xue 13:1059-63. 2007
    ..This has been further established by our in vivo model, which involves the transection or cruch of cavernous nerves and treatment with BDNF...
  76. ncbi request reprint Vascular endothelial growth factor induces IP-10 chemokine expression
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, University of California at San Francisco, San Francisco, CA 94143 1695, USA
    Biochem Biophys Res Commun 292:79-82. 2002
    ..Secretion of IP-10 into culture medium peaked at 4 h after treatment of human CSMC with 10 ng/ml of VEGF. Optimal VEGF dosage for IP-10 induction was 50 ng/ml when assayed with cells that were treated for 8 h...
  77. pmc Molecular mechanisms related to parturition-induced stress urinary incontinence
    Guiting Lin
    Department of Urology, School of Medicine, University of California, San Francisco, CA 94143, USA
    Eur Urol 55:1213-22. 2009
    ..The molecular mechanisms underlying stress urinary incontinence (SUI) at the tissue level are poorly understood...
  78. ncbi request reprint The effect of neural embryonic stem cell therapy in a rat model of cavernosal nerve injury
    Derek Bochinski
    Department of Urology, University of California, San Francisco, CA, USA
    BJU Int 94:904-9. 2004
    ..To isolate embryonic stem cells that have differentiated along the neuronal cell line, and to assess whether injecting these neural stem cells into the corpus cavernosum influences cavernosal nerve regeneration and functional status...
  79. doi request reprint Phosphodiesterase type 5 regulation in the penile corpora cavernosa
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, University of California, Department of Urology, San Francisco, CA 94115, USA
    J Sex Med 6:203-9. 2009
    ..It is hoped that a review of publications relevant to the regulation of PDE5 in the penis will be helpful to both scientists and clinicians who are interested in the sciences of erectile function/dysfunction...
  80. ncbi request reprint Upregulation of monocyte chemoattractant protein 1 and effects of transforming growth factor-beta 1 in Peyronie's disease
    Ching Shwun Lin
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA 94143 1695, USA
    Biochem Biophys Res Commun 295:1014-9. 2002
    ..Treatment with TGF-beta1-induced higher expression of MCP-1 but not CTGF in all three types of cells, with C cells being the most responsive. Based on these observations, we propose that MCP-1 could be a novel therapeutic target in PD...