Research Topics
| A P LimayeSummaryAffiliation: University of Washington Country: USA Publications
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Publications
High incidence of ganciclovir-resistant cytomegalovirus infection among lung transplant recipients receiving preemptive therapyAjit P Limaye
Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
J Infect Dis 185:20-7. 2002..All ganR isolates contained a UL97 mutation. GanR CMV infection occurs in nearly 10% of lung transplant recipients, despite preemptive antiviral therapy, and is more common among D+/R- patients...
Detection of Epstein-Barr virus DNA in sera from transplant recipients with lymphoproliferative disordersA P Limaye
Department of Laboratory Medicine and Medicine Infectious Diseases, University of Washington, Seattle, USA
J Clin Microbiol 37:1113-6. 1999..Further studies to define the role of such assays in evaluating solid-organ transplant patients at risk for PTLD are warranted...- Cytomegalovirus reactivation in critically ill immunocompetent patientsAjit P Limaye
Department of Laboratory Medicine, University of Washington Medical Center, 1959 NE Pacific St, Seattle, WA 98195 7110, USA
JAMA 300:413-22. 2008.... 
Systematic assessment of culture review as a tool to assess errors in the clinical microbiology laboratoryNancy Goodyear
Department of Laboratory Medicine, University of Washington, Box 357110, Seattle, WA 98195, USA
Arch Pathol Lab Med 132:1792-5. 2008..Daily supervisory review is a common practice in microbiology laboratories; however, there are no publications describing errors corrected by this practice...- Impact of cytomegalovirus in organ transplant recipients in the era of antiviral prophylaxisAjit P Limaye
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
Transplantation 81:1645-52. 2006..Antiviral prophylaxis has been shown to decrease the incidence of cytomegalovirus (CMV) disease in organ transplant recipients, but whether CMV disease that occurs despite prophylaxis is associated with mortality remains unknown... - Polyomavirus nephropathy in native kidneys of non-renal transplant recipientsAjit P Limaye
Departments of Laboratory Medicine and Medicine, University of Washington, Seattle, WA, USA
Am J Transplant 5:614-20. 2005..Studies to define the incidence and potential contribution of PVN to chronic renal dysfunction commonly attributed to calcineurin inhibitor toxicity in non-renal transplant recipients are warranted... - Late-onset cytomegalovirus disease in liver transplant recipients despite antiviral prophylaxisAjit P Limaye
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA
Transplantation 78:1390-6. 2004..The incidence and impact of cytomegalovirus (CMV) disease that occurs despite CMV prophylaxis among liver transplant recipients have been incompletely defined... - Antiviral resistance in cytomegalovirus: an emerging problem in organ transplant recipientsAjit P Limaye
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195, USA
Semin Respir Infect 17:265-73. 2002..Better diagnostic tools and newer, less toxic, antiviral agents with different mechanisms of action are urgently needed to decrease the morbidity associated with this complication in organ transplant recipients... - Ganciclovir-resistant cytomegalovirus in organ transplant recipientsAjit P Limaye
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195 7110, USA
Clin Infect Dis 35:866-72. 2002..Better diagnostic tools and newer, less-toxic antiviral agents with different mechanisms of action are urgently needed to decrease the morbidity associated with this complication in organ transplant recipients... - Pseudomembranous colitis caused by a toxin A(-) B(+) strain of Clostridium difficileA P Limaye
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
J Clin Microbiol 38:1696-7. 2000..We report a case of severe pseudomembranous colitis due to a toxin A(-) B(+) strain of Clostridium difficile in an immunosuppressed patient and discuss the implications for diagnostic testing in suspected C. difficile-associated diarrhea... - Cytomegalovirus (CMV) DNA load in plasma for the diagnosis of CMV disease before engraftment in hematopoietic stem-cell transplant recipientsA P Limaye
Departments of Laboratory Medicine and Medicine, University of Washington, Seattle, WA 98195 7110, USA
J Infect Dis 183:377-82. 2001..5x107] vs. <50 [range, <50-350] CMV DNA copies/mL plasma, respectively; P<.001). Quantitative PCR for CMV DNA in plasma appears to be useful for the identification of HSCT recipients at risk for CMV disease before engraftment... - Emergence of ganciclovir-resistant cytomegalovirus disease among recipients of solid-organ transplantsA P Limaye
Department of Laboratory Medicine, University of Washington, Seattle 98195 7110, USA
Lancet 356:645-9. 2000..We retrospectively assessed the occurrence of ganciclovir-resistant cytomegalovirus disease among transplant recipients who had received oral ganciclovir prophylaxis and highly potent immunosuppression... - Quantitation of BK virus load in serum for the diagnosis of BK virus-associated nephropathy in renal transplant recipientsA P Limaye
Department of Laboratory Medicine, University of Washington, Seattle, USA
J Infect Dis 183:1669-72. 2001..It is concluded that quantitative PCR for BK virus DNA in serum is useful both for identifying transplant recipients at risk for BK virus nephropathy and for monitoring the response to therapy... - Treatment of tularemia with fluoroquinolones: two cases and reviewA P Limaye
Department of Laboratory Medicine, University of Washington, Seattle, USA
Clin Infect Dis 29:922-4. 1999.... - Risk factors for late-onset cytomegalovirus disease in donor seropositive/recipient seronegative kidney transplant recipients who receive antiviral prophylaxisA A Boudreault
Department of Medicine, University of Washington, Seattle, Washington, USA
Transpl Infect Dis 13:244-9. 2011..Cytomegalovirus (CMV) disease occurs frequently after cessation of antiviral prophylaxis in CMV-seronegative kidney transplant recipients from seropositive donors (D+R-), and the risk factors are incompletely defined... - Invasive Pseudomonas aeruginosa infections: high rate of recurrence and mortality after hematopoietic cell transplantationM Hakki
Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Bone Marrow Transplant 39:687-93. 2007..aeruginosa infections are associated with high recurrence rates and mortality in this immunocompromised population. Aggressive attempts to reduce immunosuppression and to treat copathogens may help during the initial infection... - Incidence and clinical features of ganciclovir-resistant cytomegalovirus disease in heart transplant recipientsFanny Li
Department of Pharmacy Services, University of Washington, Seattle, WA 98195, USA
Clin Infect Dis 45:439-47. 2007..The incidence and clinical and virologic aspects of ganciclovir-resistant cytomegalovirus (CMV) disease have not been well-characterized in heart transplant recipients... - Herpes zoster incidence in a multicenter cohort of solid organ transplant recipientsS A Pergam
Department of Medicine, University of Washington, Seattle, Washington 98109, USA
Transpl Infect Dis 13:15-23. 2011..To assess incidence of HZ, we examined patients who underwent SOT and received follow-up care within the large multicenter US Department of Veteran's Affairs healthcare system... - Confirmation of Chagas' cardiomyopathy following heart transplantationBinh An P Phan
Division of Cardiology, Department of Medicine, University of Washington, 1914 N 34th Street, Suite 105, Seattle, WA 98103, USA
Heart Vessels 21:325-7. 2006..We review the epidemiology, cardiac pathology, and evaluation of patients with Chagas' cardiac disease. We discuss the clinical features of Chagas' cardiomyopathy and available treatments including cardiac transplantation... - Inconsistent localization of gram-positive bacteria to prostate-specific specimens from patients with chronic prostatitisJohn N Krieger
Department of Urology, University of Washington School of Medicine, Seattle, Washington 98195, USA
Urology 66:721-5. 2005..To determine the rate of gram-positive localizations and whether repetitive cultures demonstrate consistent localization of gram-positive bacteria in patients with chronic prostatitis symptoms... - Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeastsY C Chen
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
J Clin Microbiol 39:4042-51. 2001.... - DAS181 treatment of severe parainfluenza type 3 pneumonia in a lung transplant recipientD R Drozd
Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA
Transpl Infect Dis 15:E28-32. 2013..Treatment was well tolerated and associated with improvement in oxygenation and symptoms, along with rapid clearance of PIV. DAS181 should be systematically evaluated for treatment of PIV infection in transplant recipients... - Clinical and radiologic factors associated with pulmonary nodule etiology in organ transplant recipientsD H Copp
School of Medicine, University of Washington, Seattle, WA, USA
Am J Transplant 6:2759-64. 2006..9, p = 0.007). Specific clinical and radiographic features are associated with specific etiologies of PNs in SOT recipients and might be useful for guiding empiric therapy while awaiting results of definitive diagnostic studies... - Candida dubliniensis fungemia in a solid organ transplant patient: case report and review of the literatureG S Gottlieb
Department of Medicine, University of Washington Medical Center, Seattle 981 95, USA
Med Mycol 39:483-5. 2001..This case illustrates the need to be aware of emerging pathogens in new host populations and that new techniques used to identify yeast species may provide more accurate identification... - Risk factors for mortality in patients with anaerobic bacteremiaJ R Wilson
Departments of Laboratory Medicine and Medicine, University of Washington Medical Center, 1959 Northeast Pacific Street, Box 357110, Seattle, WA 98195-7110, USA
Eur J Clin Microbiol Infect Dis 23:310-6. 2004..0-1.1; P=0.005) remained significant in multivariate analysis. Among patients with anaerobic bacteremia, clinical significance varies markedly by pathogen and mortality is independently associated with age and underlying liver disease... - Nocardia veterana, a new emerging pathogenSudha Pottumarthy
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
J Clin Microbiol 41:1705-9. 2003..These cases illustrate the pathogenic potential of this newly described species and emphasize the importance of accurate identification of Nocardia isolates to the species level by integrated use of phenotypic and genotypic methods... - Invasive pneumococcal infections in adult lung transplant recipientsGuy de Bruyn
Department of Medicine, University of Washington, Washington, WA, USA
Am J Transplant 4:1366-71. 2004..Alternative prevention strategies, including use of the conjugated pneumococcal vaccine, should be explored in future studies... - Marked variability of BK virus load measurement using quantitative real-time PCR among commonly used assaysNoah G Hoffman
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
J Clin Microbiol 46:2671-80. 2008..These results suggest that both accurate BKV measurement and the uniform application of BKV screening guidelines could be significantly improved by the use of standardized reference materials and PCR primers and probes... - Rapid identification of commonly encountered Candida species directly from blood culture bottlesRangaraj Selvarangan
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195-7110, USA
J Clin Microbiol 41:5660-4. 2003..Our assay allows rapid (</=2 h) and specific detection of the most common Candida spp. directly from positive blood cultures and may facilitate delivery of optimal antifungal therapy... - Rapid identification and differentiation of Candida albicans and Candida dubliniensis by capillary-based amplification and fluorescent probe hybridizationRangaraj Selvarangan
Departments of Laboratory Medicine. Medicine. Microbiology, University of Washington Medical Center, Seattle, Washington 98195-7110, USA
J Clin Microbiol 40:4308-12. 2002..Our assay is rapid (</=2 h) and allows reliable detection and differentiation of the two germ tube-positive Candida spp... - Convenient selective differential broth for isolation of vancomycin-resistant enterococcus from fecal materialThomas J Novicki
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195-7110, USA
J Clin Microbiol 42:1637-40. 2004..We have thus developed an easily prepared vancomycin selective differential broth that is significantly more sensitive and specific in the detection of VRE than is direct fecal plating to BEA agar... - Isolation of metronidazole-resistant Bacteroides fragilis carrying the nimA nitroreductase gene from a patient in Washington StateJeffrey M Schapiro
Department of Laboratory Medicine, University of Washington Medical Center, Box 357110, 1959 NE Pacific St, Seattle, WA 98195, USA
J Clin Microbiol 42:4127-9. 2004..fragilis strains in the United States and the importance of susceptibility testing in selected situations... - Association of hepatic iron overload with invasive fungal infection in liver transplant recipientsJacob Alexander
Division of Gastroenterology, University of Washington Medical Center, Seattle, WA 98195, USA
Liver Transpl 12:1799-804. 2006..45-6.56; P = 0.003). Hepatic iron overload is strongly and independently associated with posttransplantation invasive fungal infections. Studies to confirm this finding in other centers and define the mechanism are warranted... - Strongyloidiasis in transplant patientsAlison C Roxby
Division of Allergy and Infectious Diseases, University of Washington, Seattle, USA
Clin Infect Dis 49:1411-23. 2009.... - Longitudinal assessment of cytomegalovirus (CMV)-specific immune responses in liver transplant recipients at high risk for late CMV diseaseCorinna La Rosa
Laboratory of Vaccine Research, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
J Infect Dis 195:633-44. 2007.... - Programmed death-1 expression in liver transplant recipients as a prognostic indicator of cytomegalovirus diseaseCorinna La Rosa
Laboratory of Vaccine Research, Beckman Research Institute of the City of Hope, City of Hope Comprehensive Cancer Center, Duarte, California 91010, USA
J Infect Dis 197:25-33. 2008..PD-1 up-regulation was significantly associated with incipient and overt CMV disease and with viremia. Our findings suggest that PD-1 could be developed as a prognostic tool to predict CMV disease and guide therapeutic interventions... - Calcineurin inhibitor agents interact synergistically with antifungal agents in vitro against Cryptococcus neoformans isolates: correlation with outcome in solid organ transplant recipients with cryptococcosisDimitrios P Kontoyiannis
M D Anderson Cancer Center, University of Texas, Houston, Texas 77030 3930, USA
Antimicrob Agents Chemother 52:735-8. 2008.... - Central nervous system cryptococcosis in solid organ transplant recipients: clinical relevance of abnormal neuroimaging findingsNina Singh
University of Pittsburgh, Pittsburgh, PA, USA
Transplantation 86:647-51. 2008..Prognostic implications of cryptococcal antigen and outcomes associated with central nervous system (CNS) cryptococcal lesions in solid organ transplant recipients have not been fully defined... - Pulmonary cryptococcosis in solid organ transplant recipients: clinical relevance of serum cryptococcal antigenNina Singh
University of Pittsburgh, Pennsylvania, USA
Clin Infect Dis 46:e12-8. 2008..The role of serum cryptococcal antigen in the diagnosis and determinants of antigen positivity in solid organ transplant (SOT) recipients with pulmonary cryptococcosis has not been fully defined... - Cryptococcus neoformans in organ transplant recipients: impact of calcineurin-inhibitor agents on mortalityNina Singh
University of Pittsburgh Medical Center, Pittsburgh, PA 15240, USA
J Infect Dis 195:756-64. 2007..Additionally, discerning the basis for transplant type-specific differences in disease severity has implications relevant for yielding further insights into the pathogenesis of C. neoformans infection in transplant recipients... - Late-onset invasive aspergillosis in organ transplant recipients in the current eraNina Singh
University of Pittsburgh, Pittsburgh, PA, USA
Med Mycol 44:445-9. 2006..These data have implications relevant for prophylactic strategies and guiding clinical management of transplant recipients presenting with pulmonary infiltrates... - Systemic and cerebrospinal fluid T-helper cytokine responses in organ transplant recipients with Cryptococcus neoformans infectionNina Singh
University of Pittsburgh, Pittsburgh, PA, USA
Transpl Immunol 16:69-72. 2006..The role of Th1 and Th2 mediated cytokine responses in the pathogenesis of Cryptococcus neoformans infection in organ transplant recipients has not been defined... - Combination of voriconazole and caspofungin as primary therapy for invasive aspergillosis in solid organ transplant recipients: a prospective, multicenter, observational studyNina Singh
Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15240, USA
Transplantation 81:320-6. 2006..The efficacy of the combination of voriconazole and caspofungin when used as primary therapy for invasive aspergillosis in organ transplant recipients has not been defined... - Antifungal management practices and evolution of infection in organ transplant recipients with cryptococcus neoformans infectionNina Singh
University of Pittsburgh Medical Center, Pittsburgh, PA 15240, USA
Transplantation 80:1033-9. 2005..The risk of relapse and time to recurrence is not known. We assessed antifungal treatment practices for cryptococcosis in a cohort of prospectively followed organ transplant recipients... - An immune reconstitution syndrome-like illness associated with Cryptococcus neoformans infection in organ transplant recipientsNina Singh
University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
Clin Infect Dis 40:1756-61. 2005..We describe an immune reconstitution syndrome (IRS)-like entity in the course of evolution of Cryptococcus neoformans infection in organ transplant recipients... - Treatment of refractory BK virus-associated nephropathy with cidofovirPradeep V Kadambi
Department of Internal Medicine, Pathology, University of Chicago, Chicago, IL, USA
Am J Transplant 3:186-91. 2003..Prospective, controlled trials are warranted to further define the optimal dose, toxicity and potential role of cidofovir in renal transplant recipients with BK virus nephropathy...