A P Limaye

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint High incidence of ganciclovir-resistant cytomegalovirus infection among lung transplant recipients receiving preemptive therapy
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    J Infect Dis 185:20-7. 2002
  2. pmc Quantitation of cytomegalovirus DNA load in dried blood spots correlates well with plasma viral load
    Ajit P Limaye
    Department of Medicine, University of Washington, Washington, USA
    J Clin Microbiol 51:2360-4. 2013
  3. pmc Detection of Epstein-Barr virus DNA in sera from transplant recipients with lymphoproliferative disorders
    A P Limaye
    Department of Laboratory Medicine and Medicine Infectious Diseases, University of Washington, Seattle, USA
    J Clin Microbiol 37:1113-6. 1999
  4. pmc Cytomegalovirus reactivation in critically ill immunocompetent patients
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington Medical Center, 1959 NE Pacific St, Seattle, WA 98195 7110, USA
    JAMA 300:413-22. 2008
  5. ncbi request reprint Antiviral resistance in cytomegalovirus: an emerging problem in organ transplant recipients
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195, USA
    Semin Respir Infect 17:265-73. 2002
  6. ncbi request reprint Late-onset cytomegalovirus disease in liver transplant recipients despite antiviral prophylaxis
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA
    Transplantation 78:1390-6. 2004
  7. ncbi request reprint Ganciclovir-resistant cytomegalovirus in organ transplant recipients
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195 7110, USA
    Clin Infect Dis 35:866-72. 2002
  8. ncbi request reprint Polyomavirus nephropathy in native kidneys of non-renal transplant recipients
    Ajit P Limaye
    Departments of Laboratory Medicine and Medicine, University of Washington, Seattle, WA, USA
    Am J Transplant 5:614-20. 2005
  9. ncbi request reprint Impact of cytomegalovirus in organ transplant recipients in the era of antiviral prophylaxis
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    Transplantation 81:1645-52. 2006
  10. ncbi request reprint Quantitation of BK virus load in serum for the diagnosis of BK virus-associated nephropathy in renal transplant recipients
    A P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    J Infect Dis 183:1669-72. 2001

Detail Information

Publications50

  1. ncbi request reprint High incidence of ganciclovir-resistant cytomegalovirus infection among lung transplant recipients receiving preemptive therapy
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    J Infect Dis 185:20-7. 2002
    ..All ganR isolates contained a UL97 mutation. GanR CMV infection occurs in nearly 10% of lung transplant recipients, despite preemptive antiviral therapy, and is more common among D+/R- patients...
  2. pmc Quantitation of cytomegalovirus DNA load in dried blood spots correlates well with plasma viral load
    Ajit P Limaye
    Department of Medicine, University of Washington, Washington, USA
    J Clin Microbiol 51:2360-4. 2013
    ..Future studies to optimize and evaluate this methodology for patient self-collected samples are warranted...
  3. pmc Detection of Epstein-Barr virus DNA in sera from transplant recipients with lymphoproliferative disorders
    A P Limaye
    Department of Laboratory Medicine and Medicine Infectious Diseases, University of Washington, Seattle, USA
    J Clin Microbiol 37:1113-6. 1999
    ..Further studies to define the role of such assays in evaluating solid-organ transplant patients at risk for PTLD are warranted...
  4. pmc Cytomegalovirus reactivation in critically ill immunocompetent patients
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington Medical Center, 1959 NE Pacific St, Seattle, WA 98195 7110, USA
    JAMA 300:413-22. 2008
    ....
  5. ncbi request reprint Antiviral resistance in cytomegalovirus: an emerging problem in organ transplant recipients
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195, USA
    Semin Respir Infect 17:265-73. 2002
    ..Better diagnostic tools and newer, less toxic, antiviral agents with different mechanisms of action are urgently needed to decrease the morbidity associated with this complication in organ transplant recipients...
  6. ncbi request reprint Late-onset cytomegalovirus disease in liver transplant recipients despite antiviral prophylaxis
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA
    Transplantation 78:1390-6. 2004
    ..The incidence and impact of cytomegalovirus (CMV) disease that occurs despite CMV prophylaxis among liver transplant recipients have been incompletely defined...
  7. ncbi request reprint Ganciclovir-resistant cytomegalovirus in organ transplant recipients
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195 7110, USA
    Clin Infect Dis 35:866-72. 2002
    ..Better diagnostic tools and newer, less-toxic antiviral agents with different mechanisms of action are urgently needed to decrease the morbidity associated with this complication in organ transplant recipients...
  8. ncbi request reprint Polyomavirus nephropathy in native kidneys of non-renal transplant recipients
    Ajit P Limaye
    Departments of Laboratory Medicine and Medicine, University of Washington, Seattle, WA, USA
    Am J Transplant 5:614-20. 2005
    ..Studies to define the incidence and potential contribution of PVN to chronic renal dysfunction commonly attributed to calcineurin inhibitor toxicity in non-renal transplant recipients are warranted...
  9. ncbi request reprint Impact of cytomegalovirus in organ transplant recipients in the era of antiviral prophylaxis
    Ajit P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    Transplantation 81:1645-52. 2006
    ..Antiviral prophylaxis has been shown to decrease the incidence of cytomegalovirus (CMV) disease in organ transplant recipients, but whether CMV disease that occurs despite prophylaxis is associated with mortality remains unknown...
  10. ncbi request reprint Quantitation of BK virus load in serum for the diagnosis of BK virus-associated nephropathy in renal transplant recipients
    A P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    J Infect Dis 183:1669-72. 2001
    ..It is concluded that quantitative PCR for BK virus DNA in serum is useful both for identifying transplant recipients at risk for BK virus nephropathy and for monitoring the response to therapy...
  11. ncbi request reprint Cytomegalovirus (CMV) DNA load in plasma for the diagnosis of CMV disease before engraftment in hematopoietic stem-cell transplant recipients
    A P Limaye
    Departments of Laboratory Medicine and Medicine, University of Washington, Seattle, WA 98195 7110, USA
    J Infect Dis 183:377-82. 2001
    ..5x107] vs. <50 [range, <50-350] CMV DNA copies/mL plasma, respectively; P<.001). Quantitative PCR for CMV DNA in plasma appears to be useful for the identification of HSCT recipients at risk for CMV disease before engraftment...
  12. ncbi request reprint Emergence of ganciclovir-resistant cytomegalovirus disease among recipients of solid-organ transplants
    A P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle 98195 7110, USA
    Lancet 356:645-9. 2000
    ..We retrospectively assessed the occurrence of ganciclovir-resistant cytomegalovirus disease among transplant recipients who had received oral ganciclovir prophylaxis and highly potent immunosuppression...
  13. pmc Pseudomembranous colitis caused by a toxin A(-) B(+) strain of Clostridium difficile
    A P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
    J Clin Microbiol 38:1696-7. 2000
    ..We report a case of severe pseudomembranous colitis due to a toxin A(-) B(+) strain of Clostridium difficile in an immunosuppressed patient and discuss the implications for diagnostic testing in suspected C. difficile-associated diarrhea...
  14. ncbi request reprint Treatment of tularemia with fluoroquinolones: two cases and review
    A P Limaye
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Clin Infect Dis 29:922-4. 1999
    ....
  15. doi request reprint Systematic assessment of culture review as a tool to assess errors in the clinical microbiology laboratory
    Nancy Goodyear
    Department of Laboratory Medicine, University of Washington, Box 357110, Seattle, WA 98195, USA
    Arch Pathol Lab Med 132:1792-5. 2008
    ..Daily supervisory review is a common practice in microbiology laboratories; however, there are no publications describing errors corrected by this practice...
  16. pmc Risk factors for late-onset cytomegalovirus disease in donor seropositive/recipient seronegative kidney transplant recipients who receive antiviral prophylaxis
    A A Boudreault
    Department of Medicine, University of Washington, Seattle, Washington, USA
    Transpl Infect Dis 13:244-9. 2011
    ..Cytomegalovirus (CMV) disease occurs frequently after cessation of antiviral prophylaxis in CMV-seronegative kidney transplant recipients from seropositive donors (D+R-), and the risk factors are incompletely defined...
  17. ncbi request reprint Invasive Pseudomonas aeruginosa infections: high rate of recurrence and mortality after hematopoietic cell transplantation
    M Hakki
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Bone Marrow Transplant 39:687-93. 2007
    ..aeruginosa infections are associated with high recurrence rates and mortality in this immunocompromised population. Aggressive attempts to reduce immunosuppression and to treat copathogens may help during the initial infection...
  18. doi request reprint Efficacy and safety of maribavir dosed at 100 mg orally twice daily for the prevention of cytomegalovirus disease in liver transplant recipients: a randomized, double-blind, multicenter controlled trial
    D J Winston
    Department of Medicine, UCLA Center for the Health Sciences, Los Angeles, CA, USA
    Am J Transplant 12:3021-30. 2012
    ..At a dose of 100 mg twice daily, maribavir is safe but not adequate for prevention of CMV disease in liver transplant recipients at high risk for CMV disease...
  19. doi request reprint DAS181 treatment of severe parainfluenza type 3 pneumonia in a lung transplant recipient
    D R Drozd
    Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington 98195 6175, USA
    Transpl Infect Dis 15:E28-32. 2013
    ..Treatment was well tolerated and associated with improvement in oxygenation and symptoms, along with rapid clearance of PIV. DAS181 should be systematically evaluated for treatment of PIV infection in transplant recipients...
  20. ncbi request reprint Incidence and clinical features of ganciclovir-resistant cytomegalovirus disease in heart transplant recipients
    Fanny Li
    Department of Pharmacy Services, University of Washington, Seattle, WA 98195, USA
    Clin Infect Dis 45:439-47. 2007
    ..The incidence and clinical and virologic aspects of ganciclovir-resistant cytomegalovirus (CMV) disease have not been well-characterized in heart transplant recipients...
  21. ncbi request reprint A prospective cross-sectional study of BK virus infection in non-renal solid organ transplant recipients with chronic renal dysfunction
    T D Barton
    Department of Medicine, Division of Infectious Diseases, University of Pennsylvania, Philadelphia, 19104, USA
    Transpl Infect Dis 8:102-7. 2006
    ....
  22. pmc Herpes zoster incidence in a multicenter cohort of solid organ transplant recipients
    S A Pergam
    Department of Medicine, University of Washington, Seattle, Washington 98109, USA
    Transpl Infect Dis 13:15-23. 2011
    ..To assess incidence of HZ, we examined patients who underwent SOT and received follow-up care within the large multicenter US Department of Veteran's Affairs healthcare system...
  23. ncbi request reprint Confirmation of Chagas' cardiomyopathy following heart transplantation
    Binh An P Phan
    Division of Cardiology, Department of Medicine, University of Washington, 1914 N 34th Street, Suite 105, Seattle, WA 98103, USA
    Heart Vessels 21:325-7. 2006
    ..We review the epidemiology, cardiac pathology, and evaluation of patients with Chagas' cardiac disease. We discuss the clinical features of Chagas' cardiomyopathy and available treatments including cardiac transplantation...
  24. ncbi request reprint Inconsistent localization of gram-positive bacteria to prostate-specific specimens from patients with chronic prostatitis
    John N Krieger
    Department of Urology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Urology 66:721-5. 2005
    ..To determine the rate of gram-positive localizations and whether repetitive cultures demonstrate consistent localization of gram-positive bacteria in patients with chronic prostatitis symptoms...
  25. pmc Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts
    Y C Chen
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
    J Clin Microbiol 39:4042-51. 2001
    ....
  26. doi request reprint Varicella zoster virus (VZV) and herpes simplex virus (HSV) in solid organ transplant patients
    R A Zuckerman
    Department of Medicine, Section of Infectious Disease and International Health, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA
    Am J Transplant 13:55-66; quiz 66. 2013
    ..Current antiviral therapy remains effective for the majority of clinical VZV and HSV infections...
  27. ncbi request reprint Risk factors for mortality in patients with anaerobic bacteremia
    J R Wilson
    Departments of Laboratory Medicine and Medicine, University of Washington Medical Center, 1959 Northeast Pacific Street, Box 357110, Seattle, WA 98195 7110, USA
    Eur J Clin Microbiol Infect Dis 23:310-6. 2004
    ..0-1.1; P=0.005) remained significant in multivariate analysis. Among patients with anaerobic bacteremia, clinical significance varies markedly by pathogen and mortality is independently associated with age and underlying liver disease...
  28. ncbi request reprint Candida dubliniensis fungemia in a solid organ transplant patient: case report and review of the literature
    G S Gottlieb
    Department of Medicine, University of Washington Medical Center, Seattle 981 95, USA
    Med Mycol 39:483-5. 2001
    ..This case illustrates the need to be aware of emerging pathogens in new host populations and that new techniques used to identify yeast species may provide more accurate identification...
  29. ncbi request reprint Clinical and radiologic factors associated with pulmonary nodule etiology in organ transplant recipients
    D H Copp
    School of Medicine, University of Washington, Seattle, WA, USA
    Am J Transplant 6:2759-64. 2006
    ..9, p = 0.007). Specific clinical and radiographic features are associated with specific etiologies of PNs in SOT recipients and might be useful for guiding empiric therapy while awaiting results of definitive diagnostic studies...
  30. ncbi request reprint Invasive pneumococcal infections in adult lung transplant recipients
    Guy de Bruyn
    Department of Medicine, University of Washington, Washington, WA, USA
    Am J Transplant 4:1366-71. 2004
    ..Alternative prevention strategies, including use of the conjugated pneumococcal vaccine, should be explored in future studies...
  31. pmc Nocardia veterana, a new emerging pathogen
    Sudha Pottumarthy
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
    J Clin Microbiol 41:1705-9. 2003
    ..These cases illustrate the pathogenic potential of this newly described species and emphasize the importance of accurate identification of Nocardia isolates to the species level by integrated use of phenotypic and genotypic methods...
  32. pmc Marked variability of BK virus load measurement using quantitative real-time PCR among commonly used assays
    Noah G Hoffman
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
    J Clin Microbiol 46:2671-80. 2008
    ..These results suggest that both accurate BKV measurement and the uniform application of BKV screening guidelines could be significantly improved by the use of standardized reference materials and PCR primers and probes...
  33. pmc Convenient selective differential broth for isolation of vancomycin-resistant enterococcus from fecal material
    Thomas J Novicki
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110, USA
    J Clin Microbiol 42:1637-40. 2004
    ..We have thus developed an easily prepared vancomycin selective differential broth that is significantly more sensitive and specific in the detection of VRE than is direct fecal plating to BEA agar...
  34. pmc Rapid identification of commonly encountered Candida species directly from blood culture bottles
    Rangaraj Selvarangan
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195 7110, USA
    J Clin Microbiol 41:5660-4. 2003
    ..Our assay allows rapid (</=2 h) and specific detection of the most common Candida spp. directly from positive blood cultures and may facilitate delivery of optimal antifungal therapy...
  35. pmc Strongyloidiasis in transplant patients
    Alison C Roxby
    Division of Allergy and Infectious Diseases, University of Washington, Seattle, USA
    Clin Infect Dis 49:1411-23. 2009
    ....
  36. ncbi request reprint Association of hepatic iron overload with invasive fungal infection in liver transplant recipients
    Jacob Alexander
    Division of Gastroenterology, University of Washington Medical Center, Seattle, WA 98195, USA
    Liver Transpl 12:1799-804. 2006
    ..45-6.56; P = 0.003). Hepatic iron overload is strongly and independently associated with posttransplantation invasive fungal infections. Studies to confirm this finding in other centers and define the mechanism are warranted...
  37. pmc Isolation of metronidazole-resistant Bacteroides fragilis carrying the nimA nitroreductase gene from a patient in Washington State
    Jeffrey M Schapiro
    Department of Laboratory Medicine, University of Washington Medical Center, Box 357110, 1959 NE Pacific St, Seattle, WA 98195, USA
    J Clin Microbiol 42:4127-9. 2004
    ..fragilis strains in the United States and the importance of susceptibility testing in selected situations...
  38. pmc Rapid identification and differentiation of Candida albicans and Candida dubliniensis by capillary-based amplification and fluorescent probe hybridization
    Rangaraj Selvarangan
    Departments of Laboratory Medicine Medicine Microbiology, University of Washington Medical Center, Seattle, Washington 98195 7110, USA
    J Clin Microbiol 40:4308-12. 2002
    ..Our assay is rapid (</=2 h) and allows reliable detection and differentiation of the two germ tube-positive Candida spp...
  39. doi request reprint Programmed death-1 expression in liver transplant recipients as a prognostic indicator of cytomegalovirus disease
    Corinna La Rosa
    Laboratory of Vaccine Research, Beckman Research Institute of the City of Hope, City of Hope Comprehensive Cancer Center, Duarte, California 91010, USA
    J Infect Dis 197:25-33. 2008
    ..PD-1 up-regulation was significantly associated with incipient and overt CMV disease and with viremia. Our findings suggest that PD-1 could be developed as a prognostic tool to predict CMV disease and guide therapeutic interventions...
  40. ncbi request reprint Longitudinal assessment of cytomegalovirus (CMV)-specific immune responses in liver transplant recipients at high risk for late CMV disease
    Corinna La Rosa
    Laboratory of Vaccine Research, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    J Infect Dis 195:633-44. 2007
    ....
  41. ncbi request reprint Treatment of refractory BK virus-associated nephropathy with cidofovir
    Pradeep V Kadambi
    Department of Internal Medicine, Pathology, University of Chicago, Chicago, IL, USA
    Am J Transplant 3:186-91. 2003
    ..Prospective, controlled trials are warranted to further define the optimal dose, toxicity and potential role of cidofovir in renal transplant recipients with BK virus nephropathy...
  42. pmc Central nervous system cryptococcosis in solid organ transplant recipients: clinical relevance of abnormal neuroimaging findings
    Nina Singh
    University of Pittsburgh, Pittsburgh, PA, USA
    Transplantation 86:647-51. 2008
    ..Prognostic implications of cryptococcal antigen and outcomes associated with central nervous system (CNS) cryptococcal lesions in solid organ transplant recipients have not been fully defined...
  43. ncbi request reprint Combination of voriconazole and caspofungin as primary therapy for invasive aspergillosis in solid organ transplant recipients: a prospective, multicenter, observational study
    Nina Singh
    Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15240, USA
    Transplantation 81:320-6. 2006
    ..The efficacy of the combination of voriconazole and caspofungin when used as primary therapy for invasive aspergillosis in organ transplant recipients has not been defined...
  44. pmc Calcineurin inhibitor agents interact synergistically with antifungal agents in vitro against Cryptococcus neoformans isolates: correlation with outcome in solid organ transplant recipients with cryptococcosis
    Dimitrios P Kontoyiannis
    M D Anderson Cancer Center, University of Texas, Houston, Texas 77030 3930, USA
    Antimicrob Agents Chemother 52:735-8. 2008
    ....
  45. pmc Cryptococcus neoformans in organ transplant recipients: impact of calcineurin-inhibitor agents on mortality
    Nina Singh
    University of Pittsburgh Medical Center, Pittsburgh, PA 15240, USA
    J Infect Dis 195:756-64. 2007
    ..Additionally, discerning the basis for transplant type-specific differences in disease severity has implications relevant for yielding further insights into the pathogenesis of C. neoformans infection in transplant recipients...
  46. ncbi request reprint An immune reconstitution syndrome-like illness associated with Cryptococcus neoformans infection in organ transplant recipients
    Nina Singh
    University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Clin Infect Dis 40:1756-61. 2005
    ..We describe an immune reconstitution syndrome (IRS)-like entity in the course of evolution of Cryptococcus neoformans infection in organ transplant recipients...
  47. ncbi request reprint Late-onset invasive aspergillosis in organ transplant recipients in the current era
    Nina Singh
    University of Pittsburgh, Pittsburgh, PA, USA
    Med Mycol 44:445-9. 2006
    ..These data have implications relevant for prophylactic strategies and guiding clinical management of transplant recipients presenting with pulmonary infiltrates...
  48. ncbi request reprint Systemic and cerebrospinal fluid T-helper cytokine responses in organ transplant recipients with Cryptococcus neoformans infection
    Nina Singh
    University of Pittsburgh, Pittsburgh, PA, USA
    Transpl Immunol 16:69-72. 2006
    ..The role of Th1 and Th2 mediated cytokine responses in the pathogenesis of Cryptococcus neoformans infection in organ transplant recipients has not been defined...
  49. ncbi request reprint Antifungal management practices and evolution of infection in organ transplant recipients with cryptococcus neoformans infection
    Nina Singh
    University of Pittsburgh Medical Center, Pittsburgh, PA 15240, USA
    Transplantation 80:1033-9. 2005
    ..The risk of relapse and time to recurrence is not known. We assessed antifungal treatment practices for cryptococcosis in a cohort of prospectively followed organ transplant recipients...
  50. pmc Pulmonary cryptococcosis in solid organ transplant recipients: clinical relevance of serum cryptococcal antigen
    Nina Singh
    University of Pittsburgh, Pennsylvania, USA
    Clin Infect Dis 46:e12-8. 2008
    ..The role of serum cryptococcal antigen in the diagnosis and determinants of antigen positivity in solid organ transplant (SOT) recipients with pulmonary cryptococcosis has not been fully defined...