Research Topics
| Daniel V LimSummaryAffiliation: University of South Florida Country: USA Publications
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Detail Information
Publications
Current and developing technologies for monitoring agents of bioterrorism and biowarfareDaniel V Lim
Department of Biology, Center for Biological Defense, University of South Florida, Tampa, FL 33620 5200, USA
Clin Microbiol Rev 18:583-607. 2005..Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents...
Dead-end ultrafiltration concentration and IMS/ATP-bioluminescence detection of Escherichia coli O157:H7 in recreational water and produce washDawn M Hunter
Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL 33620 7115, United States
J Microbiol Methods 87:338-42. 2011..coli O157:H7 in produce and recreational water. Accurate detection of microbial pathogens using DEUF and IMS/ATP could reduce disease outbreaks from contaminated water sources and food products...- Capture antibody targeted fluorescence in situ hybridization (CAT-FISH): dual labeling allows for increased specificity in complex samplesJoyce M Stroot
Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, 4202 E Fowler Ave ISA2015, Tampa, FL 33620, USA
J Microbiol Methods 88:275-84. 2012..With proper selection of FISH probes and capture antibodies, CAT-FISH may be used to provide rapid detection of target pathogens from within complex matrices with high levels of confidence... - Hollow-fiber ultrafiltration and PCR detection of human-associated genetic markers from various types of surface water in FloridaStephaney D Leskinen
Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, Florida 33620, USA
Appl Environ Microbiol 76:4116-7. 2010..HFUF allowed marker detection when membrane filtration did not, demonstrating HFUF's ability to facilitate detection of diluted targets by PCR in a variety of water types... 
Monitoring biosensor capture efficiencies: development of a model using GFP-expressing Escherichia coli O157:H7Joyce M Simpson-Stroot
Division of Cell Biology, Microbiology, and Molecular Biology, Department of Biology, University of South Florida, Tampa, FL 33620 5200, USA
J Microbiol Methods 72:29-37. 2008....- N-thiolated 2-oxazolidinones: a new family of antibacterial agents for methicillin-resistant Staphylococcus aureus and Bacillus anthracisRajesh Kumar Mishra
Department of Chemistry, 4202 East Fowler Avenue, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem Lett 16:2081-3. 2006..The effect of ring substituents and stereochemistry on antibacterial activity of these oxazolidinones closely parallels that previously reported for N-thiolated beta-lactam antibiotics... - Glyconanobiotics: Novel carbohydrated nanoparticle antibiotics for MRSA and Bacillus anthracisSampath C Abeylath
Center for Molecular Diversity in Drug Design, Discovery, and Delivery, Department of Chemistry, CHE 205, 4202 East Fowler Avenue, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem 16:2412-8. 2008.... - N-Thiolated beta-lactam antibacterials: effects of the N-organothio substituent on anti-MRSA activityBart Heldreth
Department of Chemistry, 4202 East Fowler Avenue, SCA 400, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem 14:3775-84. 2006..These results support the empirical model for the mechanism of action of the compounds in which the lactam transverses the bacterial membrane to deliver the organothio moiety to its cellular target... - Rapid detection and identification of bacterial pathogens by using an ATP bioluminescence immunoassayDawn M Hunter
Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Bio Science Facility Building 218, Tampa, Florida 33620 5150, USA
J Food Prot 73:739-46. 2010..This method provides a means of simultaneously detecting and identifying viable pathogens in complex matrices, and could have wider application in food microbiology... - N-Thiolated beta-lactam antibacterials: defining the role of unsaturation in the C4 side chainCristina Coates
Department of Chemistry, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem 11:193-6. 2003..This report describes the effect that unsaturation within the C(4) substituent has on antibacterial activity of these interesting new N-thiolated beta-lactams... - Effect of aryl ring fluorination on the antibacterial properties of C4 aryl-substituted N-methylthio beta-lactamsTimothy E Long
Department of Chemistry, 4202 E. Fowler Avenue-SCA400, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem 11:1859-63. 2003..In this study, we examine the effect that increasing hydrophobicity, through fluorine substitution in the C(4) aryl ring, has on the antibacterial properties... - Same-day detection of Escherichia coli O157:H7 from spinach by using electrochemiluminescent and cytometric bead array biosensorsKelly M Leach
Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, Florida 33620 5150, USA
Appl Environ Microbiol 76:8044-52. 2010..coli O157:H7 on produce in time frames that are comparable to or better than those of other testing formats. Both methods may be useful for multiplexed pathogen detection in the food industry and other testing situations... - Rapid dead-end ultrafiltration concentration and biosensor detection of enterococci from beach waters of Southern CaliforniaStephaney D Leskinen
Division of Cell Biology, Microbiology and Molecular Biology, Department of Biology, University of South Florida, 4202 E Fowler Ave, SCA 110, Tampa FL 33620 5200, USA
J Water Health 7:674-84. 2009..This field study achieved rapid detection of enterococci by ultrafiltration, secondary concentration and biosensor analysis, and demonstrates its potential usefulness for water quality monitoring... - Physical properties and biological activity of poly(butyl acrylate-styrene) nanoparticle emulsions prepared with conventional and polymerizable surfactantsJulio C Garay-Jimenez
Center for Molecular Diversity in Drug Design, Discovery, and Delivery, Department of Chemistry, University of South Florida, Tampa, Florida, USA
Nanomedicine 5:443-51. 2009.... - Penicillin-bound polyacrylate nanoparticles: restoring the activity of beta-lactam antibiotics against MRSAEdward Turos
Center for Molecular Diversity in Drug Design, Discovery, and Delivery, Department of Chemistry, 4202 East Fowler Avenue, CHE 207, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem Lett 17:3468-72. 2007..The nanoparticles have equipotent in vitro antibacterial properties against methicillin-susceptible and methicillin-resistant forms of Staphylococcus aureus and indefinite stability toward beta-lactamase... - N-thiolated beta-lactams: a new family of anti-Bacillus agentsEdward Turos
Department of Chemistry, 4202 East Fowler Avenue, CHE 207, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem Lett 16:2084-90. 2006..Elongation or extensive branching of the organothio substituent diminishes antibacterial effects, with the sec-butylthio derivative providing the strongest activity... - N-Methylthio beta-lactam antibacterials: effects of the C3/C4 ring substituents on anti-MRSA activityEdward Turos
Department of Chemistry, University of South Florida, Tampa, 33620, USA
Bioorg Med Chem 13:6289-308. 2005..Based on these results, a model for the compounds' mode of action is presented... - A rapid detection method for Vaccinia virus, the surrogate for smallpox virusKim A Donaldson
Department of Biology and Center for Biological Defense, University of South Florida, 4202 East Fowler Avenue, SCA 110, Tampa, FL 33620 5200, USA
Biosens Bioelectron 20:322-7. 2004..A photodiode quantifies the emission light at wavelengths between 670 and 710 nm. The biosensor was able to detect a minimum of 2.5 x 10(5) pfu/ml of Vaccinia virus in seeded throat culture swab specimens... - A rapid and automated fiber optic-based biosensor assay for the detection of Salmonella in spent irrigation water used in the sprouting of sprout seedsMarianne F Kramer
Department of Biology, University of South Florida, 4202 East Fowler Avenue, SCA110, Tampa, Florida 33620, USA
J Food Prot 67:46-52. 2004..Therefore, the presence of Salmonella Typhimurium in spent irrigation water could be continuously and rapidly detected 3 to 5 days before the completion of the sprouting process... - N-thiolated beta-lactams: novel antibacterial agents for methicillin-resistant Staphylococcus aureusEdward Turos
Department of Chemistry, 4202 E Fowler Avenue, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem Lett 12:2229-31. 2002..The compounds show unprecedented structure-activity features and an unusual mode of action for a beta-lactam antibiotic... - Antibiotic-conjugated polyacrylate nanoparticles: new opportunities for development of anti-MRSA agentsEdward Turos
Department of Chemistry, 4202 East Fowler Avenue, CHE 207, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem Lett 17:53-6. 2007..Additionally, the antibiotic properties of the nanoparticles can be modulated by changing the length or location of the acrylate linker on the drug monomer... - Studies on the antifungal properties of N-thiolated beta-lactamsMarci O'Driscoll
Center for Molecular Diversity in Drug Design, Discovery, and Delivery, Department of Chemistry, 4202 East Fowler Avenue, CHE 205, University of South Florida, Tampa, FL 33620, USA
Bioorg Med Chem 16:7832-7. 2008..The mode of action and structure-activity trends of these lactams as antifungals parallel that previously seen in our antibacterial studies... - Detection of Escherichia coli O157:H7 in 10- and 25-gram ground beef samples with an evanescent-wave biosensor with silica and polystyrene waveguidesDaniel R Demarco
Department of Biology, University of South Florida, Tampa 33620, USA
J Food Prot 65:596-602. 2002..In addition, when samples were spiked with high concentrations of a variety of non-E. coli O157:H7 organisms, no false positives were observed. The method was rapid, with results being obtained within 25 min of sample processing... - Rapid detection of Bacillus anthracis spores directly from powders with an evanescent wave fiber-optic biosensorT Bryan Tims
Department of Biology and Center for Biological Defense, University of South Florida, 4202 East Fowler Avenue, SCA 110, Tampa, FL 33620-5200, USA
J Microbiol Methods 59:127-30. 2004..This study describes a biosensor assay that detects B. anthracis at concentrations of 3.2 x 10(5) spores/mg or higher in spiked powders in less than 1 h with minimal sample preparation... - Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detectionJoyce M Simpson
University of South Florida, Department of Biology, Center for Biological Defense, 4202 E Fowler Ave SCA110, Tampa, 33620 5200, USA
Biosens Bioelectron 21:881-7. 2005..This paper presents methodologies to reduce the time necessary for confirmation from 10 to about 2 h, by direct PCR of bacteria from the fiber optic waveguides without the need for culture or enrichment steps... - Removal of sediment and bacteria from water using green chemistryAudrey L Buttice
Department of Chemical and Biomedical Engineering, University of South Florida, Tampa, Florida 33620, USA
Environ Sci Technol 44:3514-9. 2010..This natural material not only displays water purification abilities, but it is also affordable, renewable and readily available... - Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detectionT Bryan Tims
Department of Biology, University of South Florida, 4202 East Fowler Ave, SCA 110, Tampa, FL 33620-5200, USA
J Microbiol Methods 55:141-7. 2003..In this paper, we demonstrate a method using a rapid biosensor assay, recovery through a short enrichment, and PCR to detect and confirm the presence of at least 10(3) CFU/ml of E. coli O157:H7 in a sample in less than 10 h... - Antibody immobilization using pneumatic spray: comparison with the avidin-biotin bridge immobilization methodJhon Figueroa
Department of Chemistry, University of South Florida, 4202 E Fowler Ave, Tampa, FL 33620 5250, USA
J Immunol Methods 386:1-9. 2012..The pneumatic spray technique promises to be an alternative for the immobilization of antibodies on glass slides for capturing pathogenic targets and use in biosensor type devices... - An IMS/ATP Assay for the Detection of Mycobacterium tuberculosis in UrineDawn M Hunter
Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, 4202 E Fowler Avenue, ISA 2015, Tampa, FL 33620 7115, USA
Tuberc Res Treat 2012:292605. 2012..Sensitivity and specificity were 67.9% and 82.1%, respectively. This assay offers a possible rapid screening method for HIV-positive patients with suspected coinfection to improve MTB diagnosis... - Rapid ultrafiltration concentration and biosensor detection of enterococci from large volumes of Florida recreational waterStephaney D Leskinen
Department of Biology, University of South Florida, 4202 E Fowler Ave, SCA 110, Tampa, FL 33620 5200, USA
Appl Environ Microbiol 74:4792-8. 2008..5 h for detection compared to 24 for the standard method. This study demonstrated that enterococci can be detected rapidly using on-site ultrafiltration, secondary concentration, and biosensor analysis... - Optimization of a fluorescence sandwich enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 in apple juiceCynthia Nyquist-Battie
Department of Public Health, University of North Florida, Jacksonville, Florida 32224, USA
J Food Prot 67:2756-9. 2004..Taken all together, these results suggest that polyvinylpyrrolidone can improve detection of O157:H7 in juices by reducing the effect of polyphenols on fluorescence sandwich enzyme-linked immunosorbent assay performance... - Antibody-based detection of acid-shocked, acid-adapted, and apple juice-incubated Escherichia coli O157:H7Cynthia Nyquist-Battie
Department of Public Health, University of North Florida, 4569 St Johns Bluff Road, Jacksonville, FL 32224, USA
J Immunoassay Immunochem 26:259-71. 2005..Incubation in apple juice was not deleterious to sELISA detection. Exposure to acidic conditions can cause a small reduction in sELISA sensitivity used to detect E. coli O157:H7... - Effects of chlorine injury, starvation, and Colitag(TM) enrichment on sandwich enzyme-linked immunosorbent assay (sELISA) detection of Escherichia coli O157:H7Cynthia Nyquist-Battie
Department of Public Health, University of North Florida, 4569 St John s Bluff Road, Jacksonville, FL 32224, USA
J Immunoassay Immunochem 28:371-83. 2007..coli O157:H7. Thus, the present sELISA is valid for detection of E. coli O157:H7 in water sources, although sample matrices may interfere with assay... - Ultrasensitive detection of biomolecules with fluorescent dye-doped nanoparticlesWei Lian
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA
Anal Biochem 334:135-44. 2004..These results demonstrate the potential to apply these newly developed fluorescent nanoparticles in various biodetection systems... - Evanescent-wave biosensor for field serodiagnosis of tortoise mycoplasmosisDaniel R Brown
Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611 0880, USA
Vet Immunol Immunopathol 124:322-31. 2008....