Jane Lian

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. ncbi request reprint Transcription factors RUNX1/AML1 and RUNX2/Cbfa1 dynamically associate with stationary subnuclear domains
    Kimberly S Harrington
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Cell Sci 115:4167-76. 2002
  2. ncbi request reprint Overlapping expression of Runx1(Cbfa2) and Runx2(Cbfa1) transcription factors supports cooperative induction of skeletal development
    Nathan Smith
    The Center for Musculoskeletal Research, University of Rochester, Rochester, New York, USA
    J Cell Physiol 203:133-43. 2005
  3. pmc The WWOX tumor suppressor is essential for postnatal survival and normal bone metabolism
    Rami I Aqeilan
    Department of Molecular Virology, Immunology, and Medical Genetics, Human Cancer Genetics Program and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA
    J Biol Chem 283:21629-39. 2008
  4. pmc Cancer-related ectopic expression of the bone-related transcription factor RUNX2 in non-osseous metastatic tumor cells is linked to cell proliferation and motility
    David T Leong
    Cancer Science Institute of Singapore, National University of Singapore, Singapore
    Breast Cancer Res 12:R89. 2010
  5. pmc Runx2 mediates epigenetic silencing of the bone morphogenetic protein-3B (BMP-3B/GDF10) in lung cancer cells
    Manish Tandon
    Department of Anatomy and Cell Biology, Rush University Medical Center, Armour Academic Center, 600 S, Paulina St, Suite 507, Chicago, IL, 60612, USA
    Mol Cancer 11:27. 2012
  6. pmc MicroRNA control of bone formation and homeostasis
    Jane B Lian
    University of Massachusetts Medical School, Department of Cell Biology and Cancer Center, 55 Lake Avenue North, Room S3 326, Worcester, MA 01655, USA
    Nat Rev Endocrinol 8:212-27. 2012
  7. ncbi request reprint Contributions of nuclear architecture and chromatin to vitamin D-dependent transcriptional control of the rat osteocalcin gene
    J B Lian
    Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655 1016, USA
    Steroids 66:159-70. 2001
  8. ncbi request reprint Increased mRNA expression and protein secretion of interleukin-6 in primary human osteoblasts differentiated in vitro from rheumatoid and osteoarthritic bone
    H L Chenoufi
    Department of Orthopaedic Surgery U 2161, Rigshospitalet, DK 2100 Copenhagen, Denmark
    J Cell Biochem 81:666-78. 2001
  9. ncbi request reprint Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo
    Jane B Lian
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Cell Physiol 196:301-11. 2003
  10. ncbi request reprint Networks and hubs for the transcriptional control of osteoblastogenesis
    Jane B Lian
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Rev Endocr Metab Disord 7:1-16. 2006

Research Grants

  1. SYNTHESIS OF OSTEOCALCIN IN BONE
    Jane Lian; Fiscal Year: 2001
  2. SYNTHESIS OF OSTEOCALCIN IN BONE
    Jane Lian; Fiscal Year: 2006

Detail Information

Publications92

  1. ncbi request reprint Transcription factors RUNX1/AML1 and RUNX2/Cbfa1 dynamically associate with stationary subnuclear domains
    Kimberly S Harrington
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Cell Sci 115:4167-76. 2002
    ..We propose that the dynamic shuttling of RUNX factors in living cells to positionally stabilized foci, which is dependent on the C-terminus, is a component of the mechanism for gene regulation in vivo...
  2. ncbi request reprint Overlapping expression of Runx1(Cbfa2) and Runx2(Cbfa1) transcription factors supports cooperative induction of skeletal development
    Nathan Smith
    The Center for Musculoskeletal Research, University of Rochester, Rochester, New York, USA
    J Cell Physiol 203:133-43. 2005
    ....
  3. pmc The WWOX tumor suppressor is essential for postnatal survival and normal bone metabolism
    Rami I Aqeilan
    Department of Molecular Virology, Immunology, and Medical Genetics, Human Cancer Genetics Program and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA
    J Biol Chem 283:21629-39. 2008
    ..Our results demonstrate an essential requirement for the WWOX tumor suppressor in postnatal survival, growth, and metabolism and suggest a central role for WWOX in regulation of bone tissue formation...
  4. pmc Cancer-related ectopic expression of the bone-related transcription factor RUNX2 in non-osseous metastatic tumor cells is linked to cell proliferation and motility
    David T Leong
    Cancer Science Institute of Singapore, National University of Singapore, Singapore
    Breast Cancer Res 12:R89. 2010
    ..RUNX2 expression is inversely regulated with respect to cell growth in osteoblasts and deregulated in osteosarcoma cells...
  5. pmc Runx2 mediates epigenetic silencing of the bone morphogenetic protein-3B (BMP-3B/GDF10) in lung cancer cells
    Manish Tandon
    Department of Anatomy and Cell Biology, Rush University Medical Center, Armour Academic Center, 600 S, Paulina St, Suite 507, Chicago, IL, 60612, USA
    Mol Cancer 11:27. 2012
    ....
  6. pmc MicroRNA control of bone formation and homeostasis
    Jane B Lian
    University of Massachusetts Medical School, Department of Cell Biology and Cancer Center, 55 Lake Avenue North, Room S3 326, Worcester, MA 01655, USA
    Nat Rev Endocrinol 8:212-27. 2012
    ..The current knowledge of miRNAs characteristic of human pathologic disorders of the skeleton is presented with a future goal towards translational studies...
  7. ncbi request reprint Contributions of nuclear architecture and chromatin to vitamin D-dependent transcriptional control of the rat osteocalcin gene
    J B Lian
    Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655 1016, USA
    Steroids 66:159-70. 2001
    ....
  8. ncbi request reprint Increased mRNA expression and protein secretion of interleukin-6 in primary human osteoblasts differentiated in vitro from rheumatoid and osteoarthritic bone
    H L Chenoufi
    Department of Orthopaedic Surgery U 2161, Rigshospitalet, DK 2100 Copenhagen, Denmark
    J Cell Biochem 81:666-78. 2001
    ..This inherent property suggests that these osteoblasts, independent of local inflammatory parameters, can contribute to enhanced recruitment of osteoclast progenitors and thereby bone resorption...
  9. ncbi request reprint Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo
    Jane B Lian
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Cell Physiol 196:301-11. 2003
    ..These data indicate that Runx1 may contribute to the early stages of skeletogenesis and continues to function in the progenitor cells of tissues that support bone formation in the adult...
  10. ncbi request reprint Networks and hubs for the transcriptional control of osteoblastogenesis
    Jane B Lian
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Rev Endocr Metab Disord 7:1-16. 2006
    ..The specificity of Runx2 regulatory activities provides a basis for novel therapeutic strategies to correct bone disorders...
  11. ncbi request reprint Regulatory controls for osteoblast growth and differentiation: role of Runx/Cbfa/AML factors
    Jane B Lian
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Crit Rev Eukaryot Gene Expr 14:1-41. 2004
    ..Last, a comprehensive understanding of Runx functions in the skeleton must consider the regulatory mechanisms that control Runx2 transcription and its functional activity through posttranslational modifications...
  12. ncbi request reprint Runx2/Cbfa1: a multifunctional regulator of bone formation
    J B Lian
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Curr Pharm Des 9:2677-85. 2003
    ....
  13. ncbi request reprint Runx2/Cbfa1 functions: diverse regulation of gene transcription by chromatin remodeling and co-regulatory protein interactions
    Jane B Lian
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    Connect Tissue Res 44:141-8. 2003
    ....
  14. pmc Subnuclear targeting of Runx/Cbfa/AML factors is essential for tissue-specific differentiation during embryonic development
    J Y Choi
    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue, North Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 98:8650-5. 2001
    ..We conclude that subnuclear localization of Runx factors in specific foci together with associated regulatory functions is essential for control of Runx-dependent genes involved in tissue differentiation during embryonic development...
  15. ncbi request reprint Expression and regulation of Runx2/Cbfa1 and osteoblast phenotypic markers during the growth and differentiation of human osteoblasts
    M Prince
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Biochem 80:424-40. 2001
    ..Moreover, the data suggest that in committed human osteoblasts, constitutive expression of Cbfa1 may be required to sustain the osteoblast phenotype...
  16. ncbi request reprint Targeting of the YY1 transcription factor to the nucleolus and the nuclear matrix in situ: the C-terminus is a principal determinant for nuclear trafficking
    S McNeil
    Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA
    J Cell Biochem 68:500-10. 1998
    ..The targeted association of YY1 with the nuclear matrix provides an additional level of functional regulation for this transcription factor that can exhibit positive and negative control...
  17. ncbi request reprint Temporal evolution of gene expression in rat carotid artery following balloon angioplasty
    Jian Ming Li
    Department of Surgery, Division of Vascular Surgery, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biochem 101:399-410. 2007
    ..This study highlighted mesenchymal stromal cell cytokines-driven inflammatory extracellular matrix remodeling, as target processes for potential clinical therapeutic intervention...
  18. ncbi request reprint Nuclear microenvironments: an architectural platform for the convergence and integration of transcriptional regulatory signals
    G S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Eur J Histochem 48:65-76. 2004
    ..We address contributions of nuclear microenvironments to the convergence and integration of regulatory signals that mediate transcription by supporting the combinatorial assembly of regulatory complexes...
  19. ncbi request reprint Regulatory roles of Runx2 in metastatic tumor and cancer cell interactions with bone
    J Pratap
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Cancer Metastasis Rev 25:589-600. 2006
    ..Consideration is given to the potential for inhibition of this transcription factor as a therapeutic strategy upstream of the regulatory events contributing to the complexity of metastasis to bone...
  20. ncbi request reprint The temporal and spatial subnuclear organization of skeletal gene regulatory machinery: integrating multiple levels of transcriptional control
    J B Lian
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Calcif Tissue Int 72:631-7. 2003
  21. ncbi request reprint Insight into regulatory factor targeting to transcriptionally active subnuclear sites
    G S Stein
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, 01655, USA
    Exp Cell Res 253:110-6. 1999
    ..In this article we will present an overview of experimental approaches that provide insight into the trafficking of the hematopoietic and bone-specific AML/CBF family of regulatory factors to transcriptionally active subnuclear sites...
  22. ncbi request reprint A specific targeting signal directs Runx2/Cbfa1 to subnuclear domains and contributes to transactivation of the osteocalcin gene
    S K Zaidi
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655-0106, USA
    J Cell Sci 114:3093-102. 2001
    ....
  23. ncbi request reprint Groucho/TLE/R-esp proteins associate with the nuclear matrix and repress RUNX (CBF(alpha)/AML/PEBP2(alpha)) dependent activation of tissue-specific gene transcription
    A Javed
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA
    J Cell Sci 113:2221-31. 2000
    ..Our data are consistent with the concept that the C termini of AML proteins support activation or repression of cell-type specific genes depending on the regulatory organization of the target promoter and subnuclear localization...
  24. ncbi request reprint Transcriptional control within the three-dimensional context of nuclear architecture: requirements for boundaries and direction
    G S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Biochem . 1999
    ..Mutations that disrupt intranuclear targeting signals lead to modified subnuclear distribution of transcription factors and aberrant expression in tumor cells. J. Cell. Biochem. Suppls. 32/33:24-31, 1999...
  25. ncbi request reprint Subnuclear organization and trafficking of regulatory proteins: implications for biological control and cancer
    G S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Cell Biochem Suppl . 2000
    ..J. Cell. Biochem. Suppl. 35:84-92, 2000...
  26. ncbi request reprint Nuclear structure-gene expression interrelationships: implications for aberrant gene expression in cancer
    G S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester 01655, USA
    Cancer Res 60:2067-76. 2000
    ....
  27. ncbi request reprint Expression of cell cycle regulatory factors in differentiating osteoblasts: postproliferative up-regulation of cyclins B and E
    E Smith
    Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA
    Cancer Res 55:5019-24. 1995
    ..These results suggest that cell cycle regulatory proteins function not only during proliferation but may also play a role in normal diploid osteoblast differentiation...
  28. ncbi request reprint Expression screening of factors binding to the osteocalcin bone-specific promoter element OC box I: isolation of a novel osteoblast differentiation-specific factor
    H Hoffmann
    Department of Cell Biology, University of Massachusetts Medical Center, Worcester, Massachusetts 01655, USA
    J Cell Biochem 80:156-68. 2000
    ..The identification of DRF may further facilitate definition of gene regulatory mechanisms mediating the final stages of bone cell differentiation..
  29. ncbi request reprint Differential regulation of the two principal Runx2/Cbfa1 n-terminal isoforms in response to bone morphogenetic protein-2 during development of the osteoblast phenotype
    C Banerjee
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Endocrinology 142:4026-39. 2001
    ....
  30. pmc The abbreviated pluripotent cell cycle
    Kristina Kapinas
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    J Cell Physiol 228:9-20. 2013
    ..Wound healing, tissue engineering, and cell-based therapy to mitigate developmental aberrations illustrate applications that benefit from knowledge of the biology of the pluripotent cell cycle...
  31. pmc Runx2 association with progression of prostate cancer in patients: mechanisms mediating bone osteolysis and osteoblastic metastatic lesions
    J Akech
    The Cancer Center Prostate Cancer Discovery and Development Program, University of Massachusetts Medical School, Worcester, MA, USA
    Oncogene 29:811-21. 2010
    ..Together these findings indicate that Runx2 is a key regulator of events associated with prostate cancer metastatic bone disease...
  32. pmc runt homology domain transcription factors (Runx, Cbfa, and AML) mediate repression of the bone sialoprotein promoter: evidence for promoter context-dependent activity of Cbfa proteins
    A Javed
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0106, USA
    Mol Cell Biol 21:2891-905. 2001
    ....
  33. ncbi request reprint Linkages of nuclear architecture to biological and pathological control of gene expression
    G S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, Worcester 01655, USA
    J Cell Biochem Suppl 30:220-31. 1998
    ....
  34. ncbi request reprint Implications for interrelationships between nuclear architecture and control of gene expression under microgravity conditions
    G S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, Worcester, Massachusetts, USA
    FASEB J 13:S157-66. 1999
    ..Compromises in nuclear structure-function interrelationships can occur as a consequence of microgravity-mediated perturbations in cellular architecture...
  35. ncbi request reprint The integrated activities of IRF-2 (HiNF-M), CDP/cut (HiNF-D) and H4TF-2 (HiNF-P) regulate transcription of a cell cycle controlled human histone H4 gene: mechanistic differences between distinct H4 genes
    F Aziz
    Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA
    Mol Biol Rep 25:1-12. 1998
    ..Variations in Site II sequences among different H4 genes may accommodate differential regulation of H4 gene expression in cells and tissues with unique phenotypic properties...
  36. pmc Cells capable of bone production engraft from whole bone marrow transplants in nonablated mice
    S K Nilsson
    Cancer Center and the Department of Cell Biology, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA
    J Exp Med 189:729-34. 1999
    ..Transplanted cells were also detected as flattened bone lining cells on the periosteal bone surface...
  37. pmc The histone deacetylase inhibitor, vorinostat, reduces tumor growth at the metastatic bone site and associated osteolysis, but promotes normal bone loss
    Jitesh Pratap
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Mol Cancer Ther 9:3210-20. 2010
    ..Vorinostat treatment reduces tumor growth in bone and accompanying osteolytic disease as a result of decreased tumor burden in bone. However, vorinostat can promote osteopenia throughout the skeleton independent of tumor cell activity...
  38. ncbi request reprint Chromosomal localization of the human osteocalcin gene
    E Puchacz
    University of Massachusetts Medical Center, Department of Cell Biology, Worcester
    Endocrinology 124:2648-50. 1989
    ..Osteocalcin is a bone specific protein and it is note worthy that another osteoblast product, the bone/liver/placental alkaline phosphatase gene has also been mapped to chromosome 1...
  39. ncbi request reprint Reconstitution of Runx2/Cbfa1-null cells identifies a requirement for BMP2 signaling through a Runx2 functional domain during osteoblast differentiation
    Jong Sup Bae
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    J Cell Biochem 100:434-49. 2007
    ..Therefore, the Runx2 391-432 domain is essential for execution of the BMP2 osteogenic signal...
  40. pmc Cooperation between p27 and p107 during endochondral ossification suggests a genetic pathway controlled by p27 and p130
    Nancy Yeh
    Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Mol Cell Biol 27:5161-71. 2007
    ..The phenotypic similarities between p107(-/-) p27(D51/D51) and p107(-/-) p130(-/-) mice and the cells derived from them suggest that p27 and p130 act in an analogous pathway during chondrocyte maturation...
  41. pmc Organization of transcriptional regulatory machinery in nuclear microenvironments: implications for biological control and cancer
    Gary S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA
    Adv Enzyme Regul 47:242-50. 2007
  42. ncbi request reprint Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter
    Jose Gutierrez
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Barrio Universitario s n, Casilla 160 C, Concepcion 4079100, Chile
    J Biol Chem 282:9445-57. 2007
    ..We propose that these sequences are critical components of the regulatory mechanisms that mediate expression of this tissue-specific gene...
  43. ncbi request reprint Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton
    Christopher J Lengner
    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue North Worcester, MA 01655, USA
    Mech Dev 114:167-70. 2002
    ..Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium...
  44. ncbi request reprint BMP2 commitment to the osteogenic lineage involves activation of Runx2 by DLX3 and a homeodomain transcriptional network
    Mohammad Q Hassan
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 281:40515-26. 2006
    ..We propose that the three homeodomain proteins MSX2, DLX3, and DLX5 provide a key series of molecular switches that regulate expression of Runx2 throughout bone formation...
  45. pmc Runx2 (Cbfa1, AML-3) interacts with histone deacetylase 6 and represses the p21(CIP1/WAF1) promoter
    Jennifer J Westendorf
    Department of Orthopaedic Surgery University of Minnesota Cancer Center College of Biological Sciences Graduate Program in Microbiology, Immunology, and Cancer Biology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Cell Biol 22:7982-92. 2002
    ..These data identify Runx2 as the first transcription factor to interact with HDAC6 and suggest that HDAC6 may bind to Runx2 in differentiating osteoblasts to regulate tissue-specific gene expression...
  46. ncbi request reprint The core-binding factor beta subunit is required for bone formation and hematopoietic maturation
    Janelle Miller
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Nat Genet 32:645-9. 2002
    ..5 significant impairments in lymphopoiesis and myelopoiesis are observed. Thus, we conclude that the Cbfbeta subunit is required for HSC emergence, bone formation and normal differentiation of lymphoid and myeloid lineage cells...
  47. ncbi request reprint Chromatin remodeling and transcriptional activity of the bone-specific osteocalcin gene require CCAAT/enhancer-binding protein beta-dependent recruitment of SWI/SNF activity
    Alejandro Villagra
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Casilla 160 C, 4079100 Concepcion, Chile
    J Biol Chem 281:22695-706. 2006
    ..Together, our results indicate that the SWI/SNF complex is a key regulator of the chromatin-remodeling events that promote tissue-specific transcription in osteoblasts...
  48. ncbi request reprint Secreted frizzled related protein 1 regulates Wnt signaling for BMP2 induced chondrocyte differentiation
    Tripti Gaur
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Physiol 208:87-96. 2006
    ..Microarray gene profiling provided additional novel insights into the regulatory factors for appropriate Wnt signaling necessary for the control of chondrocyte maturation...
  49. pmc Osteoblast differentiation and skeletal development are regulated by Mdm2-p53 signaling
    Christopher J Lengner
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 172:909-21. 2006
    ....
  50. ncbi request reprint The bone-related Zn finger transcription factor Osterix promotes proliferation of mesenchymal cells
    Yeon Ju Kim
    Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 700 422, Republic of Korea
    Gene 366:145-51. 2006
    ..We propose that regulatory pathways operating upstream of or in parallel with Osterix are required for osteogenic conversion of uncommitted mesenchymal cells...
  51. ncbi request reprint Nuclear microenvironments in biological control and cancer
    Sayyed K Zaidi
    University of Massachusetts Medical School and UMASS Memorial Cancer Center, Worcester, Massachusetts, USA
    Nat Rev Cancer 7:454-63. 2007
    ..Mechanistic insights into the temporal and spatial organization of machinery for gene expression within the nucleus, which is compromised in tumours, provide a novel platform for diagnosis and therapy...
  52. ncbi request reprint Nucleosome organization and targeting of SWI/SNF chromatin-remodeling complexes: contributions of the DNA sequence
    Martin Montecino
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Casilla 160 C, Concepcion, Chile
    Biochem Cell Biol 85:419-25. 2007
    ..This discussion includes results from our group using the rat osteocalcin gene promoter as a model. Based on these results, we postulate a model for chromatin remodeling and transcriptional activation of this gene in osteoblastic cells...
  53. ncbi request reprint CCAAT/enhancer-binding proteins (C/EBP) beta and delta activate osteocalcin gene transcription and synergize with Runx2 at the C/EBP element to regulate bone-specific expression
    Soraya Gutierrez
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Biol Chem 277:1316-23. 2002
    ....
  54. ncbi request reprint Expression of the osteoblast differentiation factor RUNX2 (Cbfa1/AML3/Pebp2alpha A) is inhibited by tumor necrosis factor-alpha
    Linda Gilbert
    Division of Endocrinology and Metabolism, Emory University School of Medicine and Atlanta Veterans Affairs Medical Center, Atlanta, Georgia 30033, USA
    J Biol Chem 277:2695-701. 2002
    ..Suppression of RUNX2 by TNF may decrease osteoblast differentiation and inhibit bone formation in TNF excess states...
  55. ncbi request reprint 1,25-(OH)2-vitamin D3 suppresses the bone-related Runx2/Cbfa1 gene promoter
    Hicham Drissi
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Exp Cell Res 274:323-33. 2002
    ..This VD3-mediated suppression of Runx2 activity provides regulatory coupling between tissue-specific and steroid hormone-dependent control of genes during bone formation...
  56. pmc Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites
    Sayyed K Zaidi
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0106, USA
    Proc Natl Acad Sci U S A 99:8048-53. 2002
    ..Thus, Runx-mediated intranuclear targeting of Smads is critical for the integration of two distinct pathways essential for fetal development...
  57. ncbi request reprint Identification of novel protein/DNA interactions within the promoter of the bone-related transcription factor Runx2/Cbfa1
    Hicham Drissi
    Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655, USA
    J Cell Biochem 86:403-12. 2002
    ....
  58. pmc Runx2 regulates G protein-coupled signaling pathways to control growth of osteoblast progenitors
    Nadiya M Teplyuk
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 283:27585-97. 2008
    ....
  59. pmc Genetic and epigenetic regulation in nuclear microenvironments for biological control in cancer
    Gary S Stein
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Biochem 104:2016-26. 2008
    ....
  60. pmc Phenotypic transcription factors epigenetically mediate cell growth control
    Syed A Ali
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 105:6632-7. 2008
    ..We conclude that epigenetic control of ribosomal biogenesis by lineage-specific differentiation factors is a general developmental mechanism for coordinate control of cell growth and phenotype...
  61. ncbi request reprint Vitamin D control of gene expression: temporal and spatial parameters for organization of the regulatory machinery
    Martin Montecino
    Laboratory of Cellular and Molecular Biology, Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepcion, Casilla 160 C, Concepcion, Chile
    Crit Rev Eukaryot Gene Expr 18:163-72. 2008
    ....
  62. pmc Structural coupling of Smad and Runx2 for execution of the BMP2 osteogenic signal
    Amjad Javed
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Biol Chem 283:8412-22. 2008
    ..Our results establish the critical residues of RUNX2 for execution and completion of BMP2 signaling for osteoblastogenesis through a mechanism that requires RUNX2-SMAD transcriptional activity...
  63. ncbi request reprint 1alpha,25-dihydroxy vitamin D3-enhanced expression of the osteocalcin gene involves increased promoter occupancy of basal transcription regulators and gradual recruitment of the 1alpha,25-dihydroxy vitamin D3 receptor-SRC-1 coactivator complex
    Loreto Carvallo
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Concepcion, Chile
    J Cell Physiol 214:740-9. 2008
    ..Together, our results support a model where VDR preferentially recruits SRC-1 to enhance bone-specific OC gene transcription...
  64. pmc The Runx2 osteogenic transcription factor regulates matrix metalloproteinase 9 in bone metastatic cancer cells and controls cell invasion
    Jitesh Pratap
    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Mol Cell Biol 25:8581-91. 2005
    ..These results are consistent with Runx2 control of multiple genes that contribute to the metastatic properties of cancer cells and their activity in the bone microenvironment...
  65. ncbi request reprint Subnuclear targeting of Runx1 is required for synergistic activation of the myeloid specific M-CSF receptor promoter by PU.1
    Xiangen Li
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Biochem 96:795-809. 2005
    ..1. Taken together, our findings establish that subnuclear targeting of Runx1 is a critical component of myeloid-specific transcriptional control...
  66. ncbi request reprint Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression
    Tripti Gaur
    Department of Cell Biology and the Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Biol Chem 280:33132-40. 2005
    ..We propose that WNT/TCF1 signaling, like bone morphogenetic protein/transforming growth factor-beta signaling, activates Runx2 gene expression in mesenchymal cells for the control of osteoblast differentiation and skeletal development...
  67. ncbi request reprint Runx2 control of organization, assembly and activity of the regulatory machinery for skeletal gene expression
    Gary S Stein
    Department of Cell Biology and Cancer Center University of Massachusetts Medical School, Worcester, M 01655, USA
    Oncogene 23:4315-29. 2004
    ....
  68. ncbi request reprint Cbfbeta interacts with Runx2 and has a critical role in bone development
    Mondira Kundu
    Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, 49 Convent Drive, Building 49, Room 3A26, Bethesda, Maryland 20892, USA
    Nat Genet 32:639-44. 2002
    ..Our findings raise the possibility that mutations in CBFB may be responsible for some cases of cleidocranial dysplasia that are not linked to mutations in RUNX2...
  69. ncbi request reprint Intranuclear trafficking of transcription factors: Requirements for vitamin D-mediated biological control of gene expression
    Gary S Stein
    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, Massachusetts 01655, USA
    J Cell Biochem 88:340-55. 2003
    ....
  70. pmc Tyrosine phosphorylation controls Runx2-mediated subnuclear targeting of YAP to repress transcription
    Sayyed K Zaidi
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655 0106, USA
    EMBO J 23:790-9. 2004
    ..Our results suggest that Src/Yes signals are integrated through organization of Runx2-YAP transcriptional complexes at subnuclear sites to attenuate skeletal gene expression...
  71. pmc Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells
    Sayyed K Zaidi
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655 0105, USA
    Proc Natl Acad Sci U S A 100:14852-7. 2003
    ..Thus, mitotic partitioning and spatiotemporal reorganization of regulatory proteins together render progeny cells equivalently competent to support phenotypic gene expression...
  72. ncbi request reprint Functional architecture of the nucleus: organizing the regulatory machinery for gene expression, replication and repair
    Gary S Stein
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA
    Trends Cell Biol 13:584-92. 2003
    ..Here, we discuss how nuclear anatomy supports the temporal and spatial coordination of regulatory protein recruitment for combinatorial control...
  73. ncbi request reprint Transcriptional induction of the osteocalcin gene during osteoblast differentiation involves acetylation of histones h3 and h4
    Jiali Shen
    Department of Cell Biology, Room S3 310, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Mol Endocrinol 17:743-56. 2003
    ..We conclude that acetylation of histones H3 and H4 is functionally coupled to the chromatin remodeling events that mediate the developmental induction of OC gene transcription in bone cells...
  74. pmc Regulation of the bone-specific osteocalcin gene by p300 requires Runx2/Cbfa1 and the vitamin D3 receptor but not p300 intrinsic histone acetyltransferase activity
    Jose Sierra
    Departamento de Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Concepcion, Chile
    Mol Cell Biol 23:3339-51. 2003
    ..On the basis of these results, we propose that p300 interacts with key transcriptional regulators of the OC gene and bridges distal and proximal OC promoter sequences to facilitate responsiveness to vitamin D(3)...
  75. ncbi request reprint Cell growth regulatory role of Runx2 during proliferative expansion of preosteoblasts
    Jitesh Pratap
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Cancer Res 63:5357-62. 2003
    ..We propose that Runx2 promotes osteoblast maturation at a key developmental transition by supporting exit from the cell cycle and activating genes that facilitate bone cell phenotype development...
  76. ncbi request reprint Nuclear microenvironments support physiological control of gene expression
    Gary S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA
    Chromosome Res 11:527-36. 2003
    ....
  77. ncbi request reprint Temporal and spatial parameters of skeletal gene expression: targeting RUNX factors and their coregulatory proteins to subnuclear domains
    Gary S Stein
    Department of Cell Biology, University of Massachusetts Cancer Center, Worcester, Massachusetts 01655, USA
    Connect Tissue Res 44:149-53. 2003
    ....
  78. ncbi request reprint Intranuclear organization of RUNX transcriptional regulatory machinery in biological control of skeletogenesis and cancer
    Gary S Stein
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Blood Cells Mol Dis 30:170-6. 2003
    ....
  79. ncbi request reprint Fidelity of Runx2 activity in breast cancer cells is required for the generation of metastases-associated osteolytic disease
    George L Barnes
    Department of Orthopaedic Surgery, Boston University Medical Center, Massachusetts 02118, USA
    Cancer Res 64:4506-13. 2004
    ..These results demonstrate that Runx2 expression in breast cancer cells provides a molecular phenotype that enables the interactions between tumor cells and the bone microenvironment that lead to osteolytic disease...
  80. ncbi request reprint Primary mouse embryonic fibroblasts: a model of mesenchymal cartilage formation
    Christopher J Lengner
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0106, USA
    J Cell Physiol 200:327-33. 2004
    ..Our findings establish the MEF as a powerful tool for the generation of cartilaginous tissue ex vivo and for the study of gene function during chondrogenesis...
  81. pmc Point mutation in AML1 disrupts subnuclear targeting, prevents myeloid differentiation, and effects a transformation-like phenotype
    Diana Vradii
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 102:7174-9. 2005
    ..These findings functionally link AML1 subnuclear targeting with competency for myeloid differentiation and expression of the transformed/leukemia phenotype...
  82. ncbi request reprint Organization of transcriptional regulatory machinery in osteoclast nuclei: compartmentalization of Runx1
    Laura H Saltman
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01655, USA
    J Cell Physiol 204:871-80. 2005
    ....
  83. pmc The bone-specific expression of Runx2 oscillates during the cell cycle to support a G1-related antiproliferative function in osteoblasts
    Mario Galindo
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 280:20274-85. 2005
    ..Forced elevation of Runx2 in synchronized MC3T3 cells causes a delay in G(1). We propose that Runx2 levels and function are biologically linked to a cell growth-related G(1) transition in osteoblastic cells...
  84. pmc The dynamic organization of gene-regulatory machinery in nuclear microenvironments
    Sayyed K Zaidi
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    EMBO Rep 6:128-33. 2005
    ..When this intranuclear targeting and localization of regulatory proteins is compromised, diseases, such as cancer, can occur. A detailed understanding of this process will provide further options for diagnosis and treatment...
  85. pmc Impaired intranuclear trafficking of Runx2 (AML3/CBFA1) transcription factors in breast cancer cells inhibits osteolysis in vivo
    Amjad Javed
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 102:1454-9. 2005
    ..Our findings suggest that fidelity of Runx2 intranuclear organization is necessary for expression of target genes that mediate the osteolytic activity of metastatic breast cancer cells...
  86. ncbi request reprint Smad function and intranuclear targeting share a Runx2 motif required for osteogenic lineage induction and BMP2 responsive transcription
    Faiza Afzal
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    J Cell Physiol 204:63-72. 2005
    ..Our findings suggest that TGFbeta/ BMP2 signaling, MAPK dependent phosphorylation, and Runx2 subnuclear targeting converge to induce the osteogenic phenotype...
  87. ncbi request reprint SWI/SNF chromatin remodeling complex is obligatory for BMP2-induced, Runx2-dependent skeletal gene expression that controls osteoblast differentiation
    Daniel W Young
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655 0105, USA
    J Cell Biochem 94:720-30. 2005
    ..Taken together these results support the concept that BMP2-mediated osteogenesis requires Runx2, and demonstrates that initiation of BMP2-induced, Runx2-dependent skeletal gene expression requires SWI/SNF chromatin remodeling complexes...
  88. pmc Dlx3 transcriptional regulation of osteoblast differentiation: temporal recruitment of Msx2, Dlx3, and Dlx5 homeodomain proteins to chromatin of the osteocalcin gene
    Mohammad Q Hassan
    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Ave, North, Worcester, MA 01655 0106, USA
    Mol Cell Biol 24:9248-61. 2004
    ..We propose that multiple HD proteins in osteoblasts constitute a regulatory network that mediates development of the bone phenotype through the sequential association of distinct HD proteins with promoter regulatory elements...
  89. pmc Bone-specific transcription factor Runx2 interacts with the 1alpha,25-dihydroxyvitamin D3 receptor to up-regulate rat osteocalcin gene expression in osteoblastic cells
    Roberto Paredes
    Departamento de Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Casilla 160 C, Concepcion, Chile
    Mol Cell Biol 24:8847-61. 2004
    ....
  90. ncbi request reprint Quantitative signature for architectural organization of regulatory factors using intranuclear informatics
    Daniel W Young
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester 01655 0106, USA
    J Cell Sci 117:4889-96. 2004
    ..Our novel approach provides evidence that architectural organization of Runx factors may be fundamental to their tissue-specific regulatory function...
  91. ncbi request reprint The vitamin D response element in the distal osteocalcin promoter contributes to chromatin organization of the proximal regulatory domain
    Soraya Gutierrez
    Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655, USA
    J Biol Chem 279:43581-8. 2004
    ..Together our results support a stepwise model for chromatin remodeling of the OC promoter and show that binding of the liganded VDR.retinoid X receptor directly impacts both the distal and proximal regulatory domains...
  92. ncbi request reprint Osteoblast-related transcription factors Runx2 (Cbfa1/AML3) and MSX2 mediate the expression of bone sialoprotein in human metastatic breast cancer cells
    George L Barnes
    Department of Orthopaedic Surgery, Boston University Medical Center, Boston, Massachusetts 02118, USA
    Cancer Res 63:2631-7. 2003
    ....

Research Grants14

  1. SYNTHESIS OF OSTEOCALCIN IN BONE
    Jane Lian; Fiscal Year: 2001
    ....
  2. SYNTHESIS OF OSTEOCALCIN IN BONE
    Jane Lian; Fiscal Year: 2006
    ..These studies will establish the importance of bone selective transcription factor complexes for formation, maintenance and remodeling of bone tissue. ..