Mitchell Lewis

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. doi request reprint Allostery and the lac Operon
    Mitchell Lewis
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, 37th and Hamilton Walk, Philadelphia, PA 19104, USA
    J Mol Biol 425:2309-16. 2013
  2. pmc A tale of two repressors
    Mitchell Lewis
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Mol Biol 409:14-27. 2011
  3. pmc One is not enough
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, PA 19104 6059, USA
    J Mol Biol 392:1133-44. 2009
  4. pmc Structural analysis of lac repressor bound to allosteric effectors
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, PA 19104 6059, USA
    J Mol Biol 370:609-19. 2007
  5. doi request reprint Crystal structure of the lambda repressor and a model for pairwise cooperative operator binding
    Steven Stayrook
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, Pennsylvania 19102 6059, USA
    Nature 452:1022-5. 2008
  6. pmc Towards evolving a better repressor
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6059, USA
    Protein Eng Des Sel 22:673-83. 2009
  7. pmc A novel molecular switch
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, 19104 6059, USA
    J Mol Biol 391:661-70. 2009
  8. pmc Functional rules for lac repressor-operator associations and implications for protein-DNA interactions
    Leslie Milk
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6059, USA
    Protein Sci 19:1162-72. 2010
  9. ncbi request reprint The structural basis of cooperative regulation at an alternate genetic switch
    Heather W Pinkett
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, 19102, USA
    Mol Cell 21:605-15. 2006
  10. ncbi request reprint Structure-function relationships in 3alpha-hydroxysteroid dehydrogenases: a comparison of the rat and human isoforms
    Trevor M Penning
    Department of Pharmacology, School of Medicine, University of Pennsylvania, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    J Steroid Biochem Mol Biol 85:247-55. 2003

Research Grants

  1. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2006
  2. STRUCTURAL BIOLOGY TRAINING PROGRAM
    Mitchell Lewis; Fiscal Year: 2007
  3. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2010
  4. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2003
  5. CRYSTALLOGRAPHIC STUDIES OF THE LACTOSE REPRESSOR
    Mitchell Lewis; Fiscal Year: 2001
  6. CRYSTALLOGRAPHIC STUDIES OF THE LACTOSE REPRESSOR
    Mitchell Lewis; Fiscal Year: 1993
  7. CRYSTALLOGRAPHIC STUDIES OF THE LACTOSE REPRESSOR
    Mitchell Lewis; Fiscal Year: 1992
  8. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2011

Collaborators

Detail Information

Publications13

  1. doi request reprint Allostery and the lac Operon
    Mitchell Lewis
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, 37th and Hamilton Walk, Philadelphia, PA 19104, USA
    J Mol Biol 425:2309-16. 2013
    ..Remarkably, the principles originally put forward 50 years ago remain essentially unchanged. Models for the operon and the theory of allosteric transitions are two of the most profound discoveries of molecular biology...
  2. pmc A tale of two repressors
    Mitchell Lewis
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Mol Biol 409:14-27. 2011
    ..A historical perspective that illustrates the role these two repressor molecules played and their contribution to our understanding of gene regulation is presented...
  3. pmc One is not enough
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, PA 19104 6059, USA
    J Mol Biol 392:1133-44. 2009
    ..Understanding how effector molecules alter the thermodynamic properties of the repressor is essential for establishing a detailed understanding of gene regulation...
  4. pmc Structural analysis of lac repressor bound to allosteric effectors
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, PA 19104 6059, USA
    J Mol Biol 370:609-19. 2007
    ..The altered hydrogen bonding can account in part for the different structural conformations of the repressor, and is vital for the allosteric transition...
  5. doi request reprint Crystal structure of the lambda repressor and a model for pairwise cooperative operator binding
    Steven Stayrook
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, Pennsylvania 19102 6059, USA
    Nature 452:1022-5. 2008
    ....
  6. pmc Towards evolving a better repressor
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6059, USA
    Protein Eng Des Sel 22:673-83. 2009
    ..In addition, an operator's directionality can indirectly affect gene expression. Unraveling the basic properties of this classical epigenetic switch demonstrates that multiple factors must be optimized in designing a better switch...
  7. pmc A novel molecular switch
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, 19104 6059, USA
    J Mol Biol 391:661-70. 2009
    ..Ultimately, these novel repressors could be evolved to recognize eukaryotic promoters and used to regulate gene expression in mammalian systems...
  8. pmc Functional rules for lac repressor-operator associations and implications for protein-DNA interactions
    Leslie Milk
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6059, USA
    Protein Sci 19:1162-72. 2010
    ....
  9. ncbi request reprint The structural basis of cooperative regulation at an alternate genetic switch
    Heather W Pinkett
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 37th and Hamilton Walk, Philadelphia, 19102, USA
    Mol Cell 21:605-15. 2006
    ..Whereas the overall fold of the 186 and lambda repressor monomers is remarkably similar, the way the two repressors cooperatively assemble is quite different and explains in part the differences in their regulatory activity...
  10. ncbi request reprint Structure-function relationships in 3alpha-hydroxysteroid dehydrogenases: a comparison of the rat and human isoforms
    Trevor M Penning
    Department of Pharmacology, School of Medicine, University of Pennsylvania, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    J Steroid Biochem Mol Biol 85:247-55. 2003
    ..Instead the low k(cat) seen in AKR1C2 (50-fold less than AKR1C9) may be due to substrate "wobble" at the plastic active site...
  11. ncbi request reprint The lac repressor
    Mitchell Lewis
    School of Medicine, University of Pennsylvania, 813 Stellar Chance Building, Philadelphia, PA 19104 6059, USA
    C R Biol 328:521-48. 2005
    ..The structures of lac repressor, bound to its operator and inducer, have also been invaluable for interpreting a plethora of biochemical and genetic data...
  12. pmc Prevalence of the EH1 Groucho interaction motif in the metazoan Fox family of transcriptional regulators
    Sergey Yaklichkin
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 1110 Biomedical Research Building II III, 421 Curie Boulevard, Philadelphia, PA 19104, USA
    BMC Genomics 8:201. 2007
    ..Defining conserved functional domains that mediate the transcriptional activity of Fox proteins will contribute to a comprehensive understanding of the biological function of Fox family genes...
  13. doi request reprint Thermodynamic analysis of mutant lac repressors
    Robert Daber
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6059, USA
    J Mol Biol 409:76-87. 2011
    ....

Research Grants22

  1. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2006
    ..The long-range goal of our research is to understand the structural basis of gene regulation and how repressors, activators, and anti-terminators regulate transcription. ..
  2. STRUCTURAL BIOLOGY TRAINING PROGRAM
    Mitchell Lewis; Fiscal Year: 2007
    ..This proposal is put forward to allow the University of Pennsylvania to continue providing the highest quality training in Structural Biology and Molecular Biophysics. ..
  3. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2010
    ..In the future a modified lac repressor could be used to regulate a variety of promoters. Endogenous loci could be switched on and off to create reversible models of human disease and normal development. ..
  4. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2003
    ..The long-range goal of our research is to understand the structural basis of gene regulation and how repressors, activators, and anti-terminators regulate transcription. ..
  5. CRYSTALLOGRAPHIC STUDIES OF THE LACTOSE REPRESSOR
    Mitchell Lewis; Fiscal Year: 2001
    ..As a consequence the repressor and its complexes with operator DNA and effector molecules have both contemporary and historical importance for the understanding of gene regulation. ..
  6. CRYSTALLOGRAPHIC STUDIES OF THE LACTOSE REPRESSOR
    Mitchell Lewis; Fiscal Year: 1993
    ....
  7. CRYSTALLOGRAPHIC STUDIES OF THE LACTOSE REPRESSOR
    Mitchell Lewis; Fiscal Year: 1992
    ....
  8. Structural Studies of Transcriptional Regulators
    Mitchell Lewis; Fiscal Year: 2011
    ..In the future a modified lac repressor could be used to regulate a variety of promoters. Endogenous loci could be switched on and off to create reversible models of human disease and normal development. ..