Research Topics
| Efraim LewinsohnSummaryAffiliation: University of Michigan Country: USA Publications
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Detail Information
Publications
Genetics and biochemistry of secondary metabolites in plants: an evolutionary perspectiveE Pichersky
Biology Dept, University of Michigan, Ann Arbor, MI 48109 1048, USA
Trends Plant Sci 5:439-45. 2000..This appears to be common in secondary metabolism and might confound the assignment of gene function based on sequence information alone...- Scent engineering: toward the goal of controlling how flowers smellEran Pichersky
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Street, Ann Arbor, MI 48109, USA
Trends Biotechnol 25:105-10. 2007.... - The formation and function of plant volatiles: perfumes for pollinator attraction and defenseEran Pichersky
Molecular, Cellular and Developmental Biology, University of Michigan, 830 N University Street, Ann Arbor, Michigan 48109 1048, USA
Curr Opin Plant Biol 5:237-43. 2002.... 
Convergent evolution in plant specialized metabolismEran Pichersky
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA
Annu Rev Plant Biol 62:549-66. 2011..There are many genetic and biochemical mechanisms that can give rise to convergent evolution, and we conclude that, overall, convergent evolution in plant specialized metabolism is surprisingly common...- Raging hormones in plantsEran Pichersky
Molecular, Cellular and Developmental Biology Department, University of Michigan, 830 North University, Ann Arbor, Michigan 48109-1048, USA
Nat Chem Biol 4:584-6. 2008 
Biosynthesis of plant volatiles: nature's diversity and ingenuityEran Pichersky
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Street, Ann Arbor, MI 48109, USA
Science 311:808-11. 2006..Convergent evolution is often responsible for the ability of distally related species to synthesize the same volatile...- Characterization of geraniol synthase from the peltate glands of sweet basilYoko Iijima
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA
Plant Physiol 134:370-9. 2004.... - The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars of basilYoko Iijima
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA
Plant Physiol 136:3724-36. 2004..The relative levels of geranyl diphosphate synthase and farnesyl diphosphate synthase activities did not correlate with the total amount of terpenes produced, but showed some correlation with the ratio of monoterpenes to sesquiterpenes... - Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase familyDavid R Gang
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA
Plant Cell 14:505-19. 2002..This prediction was confirmed by site-directed mutagenesis, in which the appropriate mutants of CVOMT1 (F260S) and EOMT1 (S261F) were produced that exhibited the opposite substrate preference relative to the respective native enzyme... - The lack of floral synthesis and emission of isoeugenol in Petunia axillaris subsp. parodii is due to a mutation in the isoeugenol synthase geneTakao Koeduka
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Street, Ann Arbor, MI 48109 1048, USA
Plant J 58:961-9. 2009..hybrida by RNA interference also leads to a decrease in isoeugenol biosynthesis, but instead of the accumulation of dihydroconiferyl acetate, the flowers synthesize higher levels of eugenol... - Characterization of an acyltransferase capable of synthesizing benzylbenzoate and other volatile esters in flowers and damaged leaves of Clarkia breweriFeng Chen
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA
Plant Physiol 130:466-76. 2002.... - Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated by several esterases belonging to the AtMES esterase family of ArabidopsisYue Yang
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
Plant Physiol 147:1034-45. 2008..Our results demonstrate that MeIAA is an inactive form of IAA, and the manifestations of MeIAA in vivo activity are due to the action of free IAA that is generated from MeIAA upon hydrolysis by one or more plant esterases... - Biosynthesis and emission of terpenoid volatiles from Arabidopsis flowersFeng Chen
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
Plant Cell 15:481-94. 2003.... - The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineagesTakao Koeduka
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Street, Ann Arbor, MI 48109 1048, USA
Plant J 54:362-74. 2008..Our results indicate that plant enzymes with EGS and IGS activities have arisen multiple times and in different protein lineages... - Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol esterTakao Koeduka
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 830 North University Street, Ann Arbor, MI 48109-1048, USA
Proc Natl Acad Sci U S A 103:10128-33. 2006..The basil and petunia phenylpropene-forming enzymes belong to a structural family of NADPH-dependent reductases that also includes pinoresinol-lariciresinol reductase, isoflavone reductase, and phenylcoumaran benzylic ether reductase... - Two sesquiterpene synthases are responsible for the complex mixture of sesquiterpenes emitted from Arabidopsis flowersDorothea Tholl
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Plant J 42:757-71. 2005.... - Metabolomics, genomics, proteomics, and the identification of enzymes and their substrates and productsEyal Fridman
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA
Curr Opin Plant Biol 8:242-8. 2005..The challenges are to identify new types of enzymes and to develop biochemical techniques that are suitable for large-scale analysis... - Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chainTakao Koeduka
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
Plant Physiol 149:384-94. 2009.... - Purification and characterization of benzoate:coenzyme A ligase from Clarkia breweriTill Beuerle
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 N. University Street, Ann Arbor, MI 48109-1048, USA
Arch Biochem Biophys 400:258-64. 2002..BZL is an AMP-forming enzyme. Overall, its properties suggest that it is related to the family of CoA ligase enzymes that includes the plant enzyme 4-hydroxycinnamate:CoA ligase... - Enzymatic functions of wild tomato methylketone synthases 1 and 2Geng Yu
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
Plant Physiol 154:67-77. 2010..In contrast, the 3-ketoacid decarboxylase activity of ShMKS1, which belongs to the alpha/beta-hydrolase fold superfamily, appears to have emerged more recently, possibly within the genus Solanum... - Differential production of meta hydroxylated phenylpropanoids in sweet basil peltate glandular trichomes and leaves is controlled by the activities of specific acyltransferases and hydroxylasesDavid R Gang
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
Plant Physiol 130:1536-44. 2002..Finally, additional hydroxylase activities were identified in basil peltate glands that convert p-coumaroyl 4-hydroxyphenyllactic acid to its caffeoyl derivative and p-coumaric acid to caffeic acid... - Enzymatic synthesis and purification of aromatic coenzyme a estersTill Beuerle
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Street, Ann Arbor, Michigan 48109-1048, USA
Anal Biochem 302:305-12. 2002..The recombinant enzymes were stable for several months at -80 degrees C, thus providing a reliable and facile method to produce these delicate biological intermediates... - Analysis of the enzymatic formation of citral in the glands of sweet basilYoko Iijima
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, USA
Arch Biochem Biophys 448:141-9. 2006..These and additional data presented indicate that basil glands may contain additional dehydrogenases capable of oxidizing geraniol... - A copal-8-ol diphosphate synthase from the angiosperm Cistus creticus subsp. creticus is a putative key enzyme for the formation of pharmacologically active, oxygen-containing labdane-type diterpenesVasiliki Falara
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
Plant Physiol 154:301-10. 2010..Chemical analyses revealed that labdane-type diterpene production followed a similar pattern, with higher concentrations in trichomes of young leaves and increased accumulation upon wounding... - An aldehyde oxidase in developing seeds of Arabidopsis converts benzaldehyde to benzoic AcidMwafaq Ibdah
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
Plant Physiol 150:416-23. 2009..These findings indicate that in Arabidopsis seeds, oxidation of BD contributes in part to the synthesis of BA... - Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineoleFeng Chen
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 1048, USA
Plant Physiol 135:1956-66. 2004..These data demonstrate that divergence of organ expression pattern and product specificity are ongoing processes within the Arabidopsis TPS family... - Metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species Lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketonesEyal Fridman
Department of Molecular, Cellular, and Developmental Biology, University of Michigan An Arbor, Michigan 48109 1048, USA
Plant Cell 17:1252-67. 2005..Levels of MKS1 transcript, protein, and enzymatic activity were correlated with levels of methylketones and gland density in a variety of tomato accessions and in different plant organs... - Nicotinamidase participates in the salvage pathway of NAD biosynthesis in ArabidopsisGuodong Wang
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Street, Ann Arbor, MI 48109 1048, USA
Plant J 49:1020-9. 2007..We also show that the growth of the roots of wild-type but not nic1-1 mutant plants is inhibited and distorted by nicotinamide... - Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenolRichard Dexter
Department of Environmental Horticulture, University of Florida, Gainesville, FL 32611, USA
Plant J 49:265-75. 2007..Overall, these data support the conclusion that coniferyl acetate is the substrate of isoeugenol synthase... - Metabolic engineering of plant volatilesNatalia Dudareva
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
Curr Opin Biotechnol 19:181-9. 2008..These studies have also revealed challenges and limitations which will be likely surmounted as our understanding of plant volatile network improves... - Harnessing plant trichome biochemistry for the production of useful compoundsAnthony L Schilmiller
Departments of Biochemistry and Molecular Biology and Plant Biology, Michigan State University, East Lansing, MI 48824 1319, USA
Plant J 54:702-11. 2008..We review the literature on the biochemistry of trichomes and consider the attributes that might make them highly useful targets for plant metabolic engineering... - Characterization of a BAHD acyltransferase responsible for producing the green leaf volatile (Z)-3-hexen-1-yl acetate in Arabidopsis thalianaJohn C D'Auria
Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans Knoll Strasse 8, D 07745 Jena, Germany
Plant J 49:194-207. 2007.... - Plant volatiles: a lack of function or a lack of knowledge?Eran Pichersky
Trends Plant Sci 11:421; author reply 422-3. 2006 - Biochemistry of plant volatilesNatalia Dudareva
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
Plant Physiol 135:1893-902. 2004 - Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/Citronellol acetyltransferase in developing rose petalsMoshe Shalit
Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Plant Physiol 131:1868-76. 2003..8 kD, pI of 5.45, and is active as a monomer. The RhAAT1 gene was expressed exclusively in floral tissue with maximum transcript levels occurring at stage 4 of flower development, where scent emission is at its peak... - Reduction of benzenoid synthesis in petunia flowers reveals multiple pathways to benzoic acid and enhancement in auxin transportIrina Orlova
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907, USA
Plant Cell 18:3458-75. 2006..This suggests that BPBT is involved in metabolic processes in vegetative tissues as well... - Generation of phenylpropanoid pathway-derived volatiles in transgenic plants: rose alcohol acetyltransferase produces phenylethyl acetate and benzyl acetate in petunia flowersInna Guterman
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Plant Mol Biol 60:555-63. 2006..Feeding of transgenic petunia tissues with geraniol or octanol led to the production of their respective acetates, suggesting the dependence of volatile production on substrate availability... - Enrichment of tomato flavor by diversion of the early plastidial terpenoid pathwayRachel Davidovich Rikanati
Department of Vegetable Crops, Newe Ya ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, 30095 Israel
Nat Biotechnol 25:899-901. 2007..Monoterpene accumulation was at the expense of reduced lycopene accumulation. Similar approaches may be applicable for carotenoid-accumulating fruits and flowers of other species... - Structural basis for substrate recognition in the salicylic acid carboxyl methyltransferase familyChloe Zubieta
Structural Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Plant Cell 15:1704-16. 2003.... - The tomato homolog of CORONATINE-INSENSITIVE1 is required for the maternal control of seed maturation, jasmonate-signaled defense responses, and glandular trichome developmentLei Li
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, USA
Plant Cell 16:126-43. 2004..These findings indicate that the JA/COI1 signaling pathway regulates distinct developmental processes in different plants and suggest a role for JA in the promotion of glandular trichome-based defenses... - An enzyme-coupled colorimetric assay for S-adenosylmethionine-dependent methyltransferasesCheryl L Hendricks
Department of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213, USA
Anal Biochem 326:100-5. 2004..This assay should be generally applicable to other AdoMet-dependent methyltransferases. Moreover, the procedure is easily amendable to batch assay and high-throughput screening approaches... - Formation of monoterpenes in Antirrhinum majus and Clarkia breweri flowers involves heterodimeric geranyl diphosphate synthasesDorothea Tholl
Max Planck Institute for Chemical Ecology, D 007745 Jena, Germany
Plant Cell 16:977-92. 2004..LSU mRNA levels did not, indicating that the levels of GPPS.SSU, but not GPPS.LSU, might play a key role in regulating the formation of GPPS and, thus, monoterpene biosynthesis... - Biochemical and structural characterization of benzenoid carboxyl methyltransferases involved in floral scent production in Stephanotis floribunda and Nicotiana suaveolensMarcella B Pott
Department of Biological Sciences, University of Rostock, 18059 Rostock, Germany
Plant Physiol 135:1946-55. 2004..breweri, as shown by the models. Broad substrate specificity may indicate recent evolution or an adaptation to changing substrate availability... - Rose scent: genomics approach to discovering novel floral fragrance-related genesInna Guterman
Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Plant Cell 14:2325-38. 2002..EST sequences were submitted to the GenBank database (accession numbers BQ 103855 to BQ 106728)... - Identification of likely orthologs of tobacco salicylic acid-binding protein 2 and their role in systemic acquired resistance in Arabidopsis thalianaAnna Corina Vlot
Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA
Plant J 56:445-56. 2008..These data suggest that MeSA is a conserved SAR signal in Arabidopsis and tobacco... - Overexpression of the lemon basil alpha-zingiberene synthase gene increases both mono- and sesquiterpene contents in tomato fruitRachel Davidovich-Rikanati
Department of Vegetable Crops, Newe Ya ar Research Center, Agricultural Research Organization, P O Box 1021, Ramat Yishay 30095, Israel
Plant J 56:228-38. 2008.... - Flower proteome: changes in protein spectrum during the advanced stages of rose petal developmentMery Dafny-Yelin
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Planta 222:37-46. 2005..Interestingly, a large number of stress-related proteins were identified in developing petals. Analyses of the expression patterns of annotated proteins and their corresponding RNAs confirmed the importance of proteome characterization... - Chavicol formation in sweet basil (Ocimum basilicum): cleavage of an esterified C9 hydroxyl group with NAD(P)H-dependent reductionDaniel G Vassão
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
Org Biomol Chem 4:2733-44. 2006..The latter was shown to be the case using p-coumaryl esters as potential substrates for a NAD(P)H-dependent reductase to afford chavicol, which is then O-methylated to afford methylchavicol... - Physiology and metabolism: the second coming of plant biochemistry and physiologyEran Pichersky
Curr Opin Plant Biol 9:217-9. 2006 - An indole-3-acetic acid carboxyl methyltransferase regulates Arabidopsis leaf developmentGenji Qin
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, People s Republic of China
Plant Cell 17:2693-704. 2005..The identification of IAMT1 and the elucidation of its role in Arabidopsis leaf development have broad implications for auxin-regulated developmental process... - The Arabidopsis thaliana type I Isopentenyl Diphosphate Isomerases are targeted to multiple subcellular compartments and have overlapping functions in isoprenoid biosynthesisMichael A Phillips
Max Planck Institute for Chemical Ecology, D 07745 Jena, Germany
Plant Cell 20:677-96. 2008..Thus, each of the two Arabidopsis IPP isomerases is found in multiple but partially overlapping subcellular locations, and each can compensate for the loss of the other through partial redundancy in the cytosol... - Is the concept of regulation overused in molecular and cellular biology?Eran Pichersky
Plant Cell 17:3217-8. 2005 - Structural, biochemical, and phylogenetic analyses suggest that indole-3-acetic acid methyltransferase is an evolutionarily ancient member of the SABATH familyNan Zhao
Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee 37996, USA
Plant Physiol 146:455-67. 2008..Thus, structural, biochemical, and phylogenetic evidence supports the hypothesis that IAMT is an evolutionarily ancient member of the SABATH family likely to play a critical role in IAA homeostasis across a wide range of plants... - Harnessing plant biomass for biofuels and biomaterialsChristoph Benning
Plant J 54:533-5. 2008