Fred Levine

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Islet expression of the DNA repair enzyme 8-oxoguanosine DNA glycosylase (Ogg1) in human type 2 diabetes
    Bj rn Tyrberg
    UCSD Cancer Center, La Jolla, CA 92037 0912, USA
    BMC Endocr Disord 2:2. 2002
  2. ncbi request reprint Towards gene therapy of diabetes mellitus
    F Levine
    Department of Pediatrics, Center for Molecular Genetics, UCSD School of Medicine, La Jolla, CA 92093 0634, USA
    Mol Med Today 5:165-71. 1999
  3. ncbi request reprint beta-cell Regeneration: neogenesis, replication or both?
    Fred Levine
    UCSD School of Medicine, Pediatrics, La Jolla, CA, USA
    J Mol Med (Berl) 86:247-58. 2008
  4. ncbi request reprint β-Cell replication and islet neogenesis following partial pancreatectomy
    Seung Hee Lee
    Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, CA, USA
    Islets 3:188-95. 2011
  5. pmc HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion
    Andrew J Ball
    UCSD Department of Pediatrics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0816, USA
    Biochem Biophys Res Commun 355:331-7. 2007
  6. ncbi request reprint Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas
    Ergeng Hao
    Rebecca and John Moores UCSD Cancer Center, University of California San Diego, 9500 Gilman Drive, MC 0816, La Jolla, California 92093, USA
    Nat Med 12:310-6. 2006
  7. pmc Id3 upregulates BrdU incorporation associated with a DNA damage response, not replication, in human pancreatic β-cells
    Seung Hee Lee
    Sanford Children s Health Research Center, Sanford Burnham Institute for Medical Research, La Jolla, CA, USA
    Islets 3:358-66. 2011
  8. doi request reprint The Id3/E47 axis mediates cell-cycle control in human pancreatic ducts and adenocarcinoma
    Seung Hee Lee
    Department of Pediatrics, Universityof California San Diego, La Jolla, CA 92093, USA
    Mol Cancer Res 9:782-90. 2011
  9. ncbi request reprint Sources of beta-cells for human cell-based therapies for diabetes
    Pamela Itkin-Ansari
    Cancer Center, University of California San Diego School of Medicine, La Jolla, CA, USA
    Cell Biochem Biophys 40:103-12. 2004
  10. pmc Islet specific Wnt activation in human type II diabetes
    Seung Hee Lee
    Development and Aging Program, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Exp Diabetes Res 2008:728763. 2008

Research Grants

Collaborators

Detail Information

Publications30

  1. pmc Islet expression of the DNA repair enzyme 8-oxoguanosine DNA glycosylase (Ogg1) in human type 2 diabetes
    Bj rn Tyrberg
    UCSD Cancer Center, La Jolla, CA 92037 0912, USA
    BMC Endocr Disord 2:2. 2002
    ..An increase of Ogg1 in islet cell mitochondria is consistent with a model in which hyperglycemia and consequent increased beta-cell oxidative metabolism lead to DNA damage and the induction of Ogg1 expression...
  2. ncbi request reprint Towards gene therapy of diabetes mellitus
    F Levine
    Department of Pediatrics, Center for Molecular Genetics, UCSD School of Medicine, La Jolla, CA 92093 0634, USA
    Mol Med Today 5:165-71. 1999
    ..This review discusses various approaches to modifying cells genetically, both in vitro and in vivo, for the treatment of diabetes...
  3. ncbi request reprint beta-cell Regeneration: neogenesis, replication or both?
    Fred Levine
    UCSD School of Medicine, Pediatrics, La Jolla, CA, USA
    J Mol Med (Berl) 86:247-58. 2008
    ..Clearly, understanding the nature and control of beta-cell regeneration is critical for success in efforts to treat diabetes by beta-cell replacement...
  4. ncbi request reprint β-Cell replication and islet neogenesis following partial pancreatectomy
    Seung Hee Lee
    Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, CA, USA
    Islets 3:188-95. 2011
    ..In summary, our data indicate that islet neogenesis following partial pancreatectomy does not occur...
  5. pmc HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion
    Andrew J Ball
    UCSD Department of Pediatrics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0816, USA
    Biochem Biophys Res Commun 355:331-7. 2007
    ..We conclude that the balance of Notch activators and inhibitors may play an important role in maintaining the beta-cell differentiated state...
  6. ncbi request reprint Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas
    Ergeng Hao
    Rebecca and John Moores UCSD Cancer Center, University of California San Diego, 9500 Gilman Drive, MC 0816, La Jolla, California 92093, USA
    Nat Med 12:310-6. 2006
    ..Nonendocrine-to-endocrine differentiation of NEPECs supports the existence of endocrine stem or progenitor cells within the epithelial compartment of the adult human pancreas...
  7. pmc Id3 upregulates BrdU incorporation associated with a DNA damage response, not replication, in human pancreatic β-cells
    Seung Hee Lee
    Sanford Children s Health Research Center, Sanford Burnham Institute for Medical Research, La Jolla, CA, USA
    Islets 3:358-66. 2011
    ..The data provide a much needed comparative model for investigating the molecular basis for this resistance in order to develop a strategy for improving replication competence in β-cells...
  8. doi request reprint The Id3/E47 axis mediates cell-cycle control in human pancreatic ducts and adenocarcinoma
    Seung Hee Lee
    Department of Pediatrics, Universityof California San Diego, La Jolla, CA 92093, USA
    Mol Cancer Res 9:782-90. 2011
    ..Collectively, the data establish dysregulation of the Id/bHLH axis as an early and sustained feature of ductal pathogenesis and mark this axis as a potential therapeutic target for intervention in pancreatitis and PDA...
  9. ncbi request reprint Sources of beta-cells for human cell-based therapies for diabetes
    Pamela Itkin-Ansari
    Cancer Center, University of California San Diego School of Medicine, La Jolla, CA, USA
    Cell Biochem Biophys 40:103-12. 2004
    ..Further understanding of the factors governing beta-cell development are likely to be required before a robust cell-based therapy is available for the treatment of diabetes...
  10. pmc Islet specific Wnt activation in human type II diabetes
    Seung Hee Lee
    Development and Aging Program, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Exp Diabetes Res 2008:728763. 2008
    ..These data support a model in which the Wnt pathway plays a dynamic role in the pathogenesis of type II diabetes and suggest manipulation of Wnt signaling as a new approach to beta-cell-directed diabetes therapy...
  11. ncbi request reprint Cell-based therapies for diabetes: progress towards a transplantable human beta cell line
    Pamela Itkin-Ansari
    Cancer Center, University of California, San Diego, La Jolla, California 92093, USA
    Ann N Y Acad Sci 1005:138-47. 2003
    ..Here, we provide further information on the characteristics of cell lines that we have developed from the human pancreas that are relevant to the development of a beta cell replacement therapy for diabetes...
  12. pmc Phenothiazine neuroleptics signal to the human insulin promoter as revealed by a novel high-throughput screen
    Alice Kiselyuk
    Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA
    J Biomol Screen 15:663-70. 2010
    ....
  13. ncbi request reprint Telomere-independent cellular senescence in human fetal cardiomyocytes
    Andrew J Ball
    UCSD Cancer Center, 9500 Gilman Drive, La Jolla, CA 92093 0816, USA
    Aging Cell 4:21-30. 2005
    ..In addition to offering novel insights into the behavior of human fetal cardiomyocytes in culture, these findings have implications for the development of a cell-based therapy for cardiac injury using primary fetal heart tissue...
  14. pmc CENP-A, a protein required for chromosome segregation in mitosis, declines with age in islet but not exocrine cells
    Seung Hee Lee
    Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA
    Aging (Albany NY) 2:785-90. 2010
    ..This finding has implications for efforts to induce beta-cell replication as a treatment for diabetes...
  15. pmc Coordinated regulation by Shp2 tyrosine phosphatase of signaling events controlling insulin biosynthesis in pancreatic beta-cells
    Sharon S Zhang
    Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:7531-6. 2009
    ..Therefore, Shp2 acts as a signal coordinator in beta-cells, orchestrating multiple pathways controlling insulin biosynthesis to maintain glucose homeostasis...
  16. ncbi request reprint Chronic treatment of mitochondrial disease patients with dichloroacetate
    Bruce A Barshop
    Department of Pediatrics, University of California San Diego, La Jolla, CA 92093 0830, USA
    Mol Genet Metab 83:138-49. 2004
    ....
  17. ncbi request reprint Gene therapy for diabetes
    C Demeterco
    UCSD Cancer Center, La Jolla, CA. 92093-0912, USA
    Front Biosci 6:D175-91. 2001
    ..The focus of this review is on how gene therapy can be used in beta cell replacement strategies. Gene transfer to beta cells as well as recent advances in beta cell growth and development will be discussed...
  18. ncbi request reprint NeuroD1 in the endocrine pancreas: localization and dual function as an activator and repressor
    P Itkin-Ansari
    Cancer Center, University of California, San Diego, School of Medicine, Stem Cell Program, The Burnham Institute, La Jolla, California 92093 0816, USA
    Dev Dyn 233:946-53. 2005
    ....
  19. ncbi request reprint Current and future treatment strategies for type 2 diabetes: the beta-cell as a therapeutic target
    B Tyrberg
    UCSD Cancer Center, La Jolla, CA 92037 0912, USA
    Curr Opin Investig Drugs 2:1568-74. 2001
    ..The treatment strategies presented include promotion of beta-cell proliferation and differentiation by glucagon-like peptide 1 receptor agonists...
  20. pmc HNF4α antagonists discovered by a high-throughput screen for modulators of the human insulin promoter
    Alice Kiselyuk
    Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
    Chem Biol 19:806-18. 2012
    ..The discovery of bioactive modulators for HNF4α raises the possibility that diseases involving HNF4α, such as diabetes and cancer, might be amenable to pharmacologic intervention by modulation of HNF4α activity...
  21. ncbi request reprint Cryopreservation by slow cooling with DMSO diminished production of Oct-4 pluripotency marker in human embryonic stem cells
    Igor I Katkov
    UCSD Cancer Center, San Diego, CA 92122, USA Stem Cell Center Core, Burnham Institute for Medical Research BIMR, La Jolla, CA 92037, USA
    Cryobiology 53:194-205. 2006
    ..It is concluded that new protocols and media are required for freezing hESC and safe storage at -80 degrees C as well as studies of the mechanisms of stress-related events associated with cell cryopreservation...
  22. ncbi request reprint Diabetes mellitus-cell transplantation and gene therapy approaches
    T Halvorsen
    UCSD Cancer Center, La Jolla, CA 92093-0912, USA
    Curr Mol Med 1:273-86. 2001
    ..This review focuses on approaches to developing robust and widely applicable beta-cell replacement strategies with an emphasis on manipulating beta-cell growth and differentiation by genetic engineering...
  23. doi request reprint Efficient β-cell regeneration by a combination of neogenesis and replication following β-cell ablation and reversal of pancreatic duct ligation
    Ergeng Hao
    Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, California, USA Pediatric Diabetes Research Center, University of California, San Diego, California, USA
    Stem Cells 31:2388-95. 2013
    ..This provides an important proof of principle that efficient β-cell regeneration is possible, even from a starting point of profound β-cell ablation. This has important implications for efforts to promote β-cell regeneration...
  24. ncbi request reprint E2A gene products are not required for insulin gene expression
    P Itkin-Ansari
    Center for Molecular Genetics, Whittier Institute, Department of Pediatrics, University of California San Diego School of Medicine 92093 0634, USA
    Endocrinology 137:3540-3. 1996
    ..These data show that although E2A gene products probably play an important role in insulin gene expression, pancreatic development and insulin production can proceed in their absence...
  25. doi request reprint Induction of β-cell replication by a synthetic HNF4α antagonist
    Seung Hee Lee
    Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, California, USA
    Stem Cells 31:2396-407. 2013
    ....
  26. ncbi request reprint c-Myc controls proliferation versus differentiation in human pancreatic endocrine cells
    Carla Demeterco
    Cancer Center, University of California San Diego, 1st Floor Building 7, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Clin Endocrinol Metab 87:3475-85. 2002
    ..These results suggest that c-Myc plays a central role in a cell-cell contact-mediated switch mechanism by which cell division vs. differentiation in endocrine cells is determined...
  27. pmc Derivation of a retinoid X receptor scaffold from peroxisome proliferator-activated receptor gamma ligand 1-Di(1H-indol-3-yl)methyl-4-trifluoromethylbenzene
    Marcia I Dawson
    Cancer Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Rd, La Jolla, CA 92037, USA
    ChemMedChem 4:1106-19. 2009
    ....
  28. ncbi request reprint Characterization of ataxia telangiectasia fibroblasts with extended life-span through telomerase expression
    L D Wood
    Department of Biology, University of California San Diego, La Jolla, California, CA 92093-0322, USA
    Oncogene 20:278-88. 2001
    ..These immortalized A-T cells provide a more stable cell system to investigate the molecular mechanisms underlying the cellular phenotypes of Ataxia-telangiectasia...
  29. ncbi request reprint Presenilin 1 facilitates the constitutive turnover of beta-catenin: differential activity of Alzheimer's disease-linked PS1 mutants in the beta-catenin-signaling pathway
    D E Kang
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 19:4229-37. 1999
    ..These findings raise the intriguing possibility that PS1-beta-catenin interactions and subsequent activities may be consequential for the pathogenesis of AD...
  30. ncbi request reprint No pancreatic endocrine stem cells?
    Fred Levine
    Burnham Institute and the University of California, San Diego, USA
    N Engl J Med 351:1024-6. 2004

Research Grants1

  1. High-Throughput Screen for Beta-Cell Replication
    Fred Levine; Fiscal Year: 2006
    ..By furthering our understanding of the mechanisms that control cell cycle entry in human beta-cells, we should be in an improved position to induce beta-cell regeneration in vivo and/or in vitro. ..