- Extracellular polymerization of 3-hydroxyalkanoate monomers with the polymerase of Alcaligenes eutrophusR W Lenz
Polymer Science and Engineering Department, University of Massachusetts, Amherst 01003, USA
Int J Biol Macromol 25:55-60. 1999..K(M) values were determined for the activity of the polymerase with both HBCoA and HVCoA, and the rates of propagation for both monomers were estimated. With a racemic mixture of HBCoA, the enzyme polymerized only the [R] monomer...
- Bacterial polyesters: biosynthesis, biodegradable plastics and biotechnologyRobert W Lenz
Polymer Science and Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003 4530, USA
Biomacromolecules 6:1-8. 2005..In this historical review, the chemical, biochemical and microbial highlights are linked to personalities and locations involved with the events covering a discovery timespan of 75 years...
- Poly(hydroxyalkanoic acid) Biosynthesis in Ectothiorhodospirashaposhnikovii: Characterization and Reactivity of a Type III PHA SynthaseShiming Zhang
Department of Microbiology and Polymer Science and Engineering Department, University of Massachusetts, Amherst, 01003, USA
Biomacromolecules 5:40-8. 2004..shaposhnikovii may catalyze a living polymerization and demonstrate that two PhaC and two PhaE subunits comprise a single catalytic site in the synthase complex...
- Mechanism of the polymerization reaction initiated and catalyzed by the polyhydroxybutyrate synthase of Ralstonia eutrophaShiming Zhang
Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA
Biomacromolecules 4:504-9. 2003..These two properties explain the nature of lag phenomenon during the in vitro polymerization reaction catalyzed by this enzyme..