MARK LEHRMAN

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi request reprint Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis
    Ningguo Gao
    Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 415:3-20. 2006
  2. ncbi request reprint Teaching dolichol-linked oligosaccharides more tricks with alternatives to metabolic radiolabeling
    Mark A Lehrman
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Glycobiology 17:75R-85R. 2007
  3. ncbi request reprint Stimulation of N-linked glycosylation and lipid-linked oligosaccharide synthesis by stress responses in metazoan cells
    Mark A Lehrman
    Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Crit Rev Biochem Mol Biol 41:51-75. 2006
  4. ncbi request reprint Rapid activation of glycogen phosphorylase by the endoplasmic reticulum unfolded protein response
    Arvind Gill
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 277:44747-53. 2002
  5. pmc Translation attenuation by PERK balances ER glycoprotein synthesis with lipid-linked oligosaccharide flux
    Jie Shang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 176:605-16. 2007
  6. ncbi request reprint Extension of lipid-linked oligosaccharides is a high-priority aspect of the unfolded protein response: endoplasmic reticulum stress in Type I congenital disorder of glycosylation fibroblasts
    Jie Shang
    Department of Pharmacology, Univerity of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9041, USA
    Glycobiology 12:307-17. 2002
  7. ncbi request reprint Unexpected basis for impaired Glc3Man9GlcNAc2-P-P-dolichol biosynthesis by elevated expression of GlcNAc-1-P transferase
    Ningguo Gao
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Glycobiology 18:125-34. 2008
  8. ncbi request reprint Coupling of the dolichol-P-P-oligosaccharide pathway to translation by perturbation-sensitive regulation of the initiating enzyme, GlcNAc-1-P transferase
    Ningguo Gao
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 277:39425-35. 2002
  9. pmc Analysis of glycosylation in CDG-Ia fibroblasts by fluorophore-assisted carbohydrate electrophoresis: implications for extracellular glucose and intracellular mannose 6-phosphate
    Ningguo Gao
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 280:17901-9. 2005
  10. ncbi request reprint Characterization of lipid-linked oligosaccharide accumulation in mouse models of Batten disease
    Steve K Cho
    Department of Internal Medicine and Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Glycobiology 15:637-48. 2005

Research Grants

  1. N-Glycosylation And ER Stress
    MARK LEHRMAN; Fiscal Year: 2009
  2. MOLECULAR BIOLOGY OF ASPARAGINE LINKED GLYCOSYLATION
    MARK LEHRMAN; Fiscal Year: 2002
  3. LKB1-Independent Metformin Response
    MARK LEHRMAN; Fiscal Year: 2006
  4. MOLECULAR BIOLOGY OF ASPARAGINE-LINKED GLYCOSYLATION
    MARK LEHRMAN; Fiscal Year: 2006
  5. MOLECULAR BIOLOGY OF ASPARAGINE-LINKED GLYCOSYLATION
    MARK LEHRMAN; Fiscal Year: 1993
  6. N-Glycosylation And ER Stress
    MARK ANDREW LEHRMAN; Fiscal Year: 2010

Collaborators

Detail Information

Publications16

  1. ncbi request reprint Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis
    Ningguo Gao
    Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 415:3-20. 2006
    ..With FACE, steady-state LLO compositions can be determined quantitatively from cell cultures and animal tissues. We also present FACE methods for analysis of phosphosugars and nucleotide sugars, which are metabolic precursors of LLOs...
  2. ncbi request reprint Teaching dolichol-linked oligosaccharides more tricks with alternatives to metabolic radiolabeling
    Mark A Lehrman
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Glycobiology 17:75R-85R. 2007
    ..New information revealed by these methods with regard to regulation, genetic disorders, and evolution of the dolichol cycle, as well as caveats of radiolabeling techniques, will be discussed...
  3. ncbi request reprint Stimulation of N-linked glycosylation and lipid-linked oligosaccharide synthesis by stress responses in metazoan cells
    Mark A Lehrman
    Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Crit Rev Biochem Mol Biol 41:51-75. 2006
    ..This information will interest readers who study the biological roles of stress responses, the functions of N-linked glycans, and potential strategies for treatment of genetic disorders of N-linked glycosylation...
  4. ncbi request reprint Rapid activation of glycogen phosphorylase by the endoplasmic reticulum unfolded protein response
    Arvind Gill
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 277:44747-53. 2002
    ....
  5. pmc Translation attenuation by PERK balances ER glycoprotein synthesis with lipid-linked oligosaccharide flux
    Jie Shang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 176:605-16. 2007
    ..Thus, by sensing ER stress from defective glycosylation, PERK can restore ER homeostasis by balancing polypeptide synthesis with flux through the LLO pathway...
  6. ncbi request reprint Extension of lipid-linked oligosaccharides is a high-priority aspect of the unfolded protein response: endoplasmic reticulum stress in Type I congenital disorder of glycosylation fibroblasts
    Jie Shang
    Department of Pharmacology, Univerity of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9041, USA
    Glycobiology 12:307-17. 2002
    ..This suggests that cells stimulate N-glycosylation as part of a first line of defense against ER dysfunction. The broader implications of these results for the biological significance of the UPR are discussed...
  7. ncbi request reprint Unexpected basis for impaired Glc3Man9GlcNAc2-P-P-dolichol biosynthesis by elevated expression of GlcNAc-1-P transferase
    Ningguo Gao
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Glycobiology 18:125-34. 2008
    ..Since the mammalian GPT gene can undergo spontaneous amplification, the data also indicate a potential basis for forms of pseudo-CDG-If...
  8. ncbi request reprint Coupling of the dolichol-P-P-oligosaccharide pathway to translation by perturbation-sensitive regulation of the initiating enzyme, GlcNAc-1-P transferase
    Ningguo Gao
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 277:39425-35. 2002
    ..The implications of these results for the organization of the LLO pathway are discussed...
  9. pmc Analysis of glycosylation in CDG-Ia fibroblasts by fluorophore-assisted carbohydrate electrophoresis: implications for extracellular glucose and intracellular mannose 6-phosphate
    Ningguo Gao
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 280:17901-9. 2005
    ..The possibilities that M6P may accumulate in hepatocytes and that M6P-stimulated LLO cleavage may account for both hypoglycosylation and the clinical failure of dietary mannose therapy with CDG-Ia patients are discussed...
  10. ncbi request reprint Characterization of lipid-linked oligosaccharide accumulation in mouse models of Batten disease
    Steve K Cho
    Department of Internal Medicine and Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Glycobiology 15:637-48. 2005
    ..3% of the autofluorescent storage material by mass. The accumulation of LLOs is postulated to result from inhibition of late stages of lysosomal degradation of autophagosomes, which may be enriched in these metabolic precursors...
  11. ncbi request reprint Discordance of UPR signaling by ATF6 and Ire1p-XBP1 with levels of target transcripts
    Jie Shang
    Department of Pharmacology, University of Texas, Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
    Biochem Biophys Res Commun 317:390-6. 2004
    ....
  12. ncbi request reprint Activation of glycogen phosphorylase with 5-aminoimidazole-4-carboxamide riboside (AICAR). Assessment of glycogen as a precursor of mannosyl residues in glycoconjugates
    Jie Shang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    J Biol Chem 279:12076-80. 2004
    ..Using this strategy, we show that glycogen can be a significant and regulatable precursor of mannosyl units in lipid-linked oligosaccharides and glycoproteins...
  13. ncbi request reprint Metformin-stimulated mannose transport in dermal fibroblasts
    Jie Shang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 279:9703-12. 2004
    ..This suggests that AMP-activated protein kinase may be a regulator of mannose metabolism and implies a therapy for congenital disorders of glycosylation-Ia...
  14. ncbi request reprint Analyses of dolichol pyrophosphate-linked oligosaccharides in cell cultures and tissues by fluorophore-assisted carbohydrate electrophoresis
    Ningguo Gao
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas 75390 9041, USA
    Glycobiology 12:353-60. 2002
    ..In summary, FACE is a facile, accurate, and sensitive method for LLO analysis, permitting investigations not feasible by metabolic labeling...
  15. ncbi request reprint Under normoxia, 2-deoxy-D-glucose elicits cell death in select tumor types not by inhibition of glycolysis but by interfering with N-linked glycosylation
    Metin Kurtoglu
    Department of Cell Biologoy and Anatomy, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA
    Mol Cancer Ther 6:3049-58. 2007
    ....
  16. ncbi request reprint Recycling of dolichyl monophosphate to the cytoplasmic leaflet of the endoplasmic reticulum after the cleavage of dolichyl pyrophosphate on the lumenal monolayer
    Jeffrey S Rush
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, Kentucky 40536, USA
    J Biol Chem 283:4087-93. 2008
    ....

Research Grants23

  1. N-Glycosylation And ER Stress
    MARK LEHRMAN; Fiscal Year: 2009
    ..Third, we will evaluate a potential host- defense mechanism against certain infectious human viruses. ..
  2. MOLECULAR BIOLOGY OF ASPARAGINE LINKED GLYCOSYLATION
    MARK LEHRMAN; Fiscal Year: 2002
    ..V. Measure the selectivity of the existing Lec35 cDNA for mannose-P-dolichol over glucose-P-dolichol (GPD), and isolate a cDNA for a GPD-selective form of Lec35. ..
  3. LKB1-Independent Metformin Response
    MARK LEHRMAN; Fiscal Year: 2006
    ..This will make possible a future R01 application to determine the role of LIMR in metformin's antihyperglycemic activity. ..
  4. MOLECULAR BIOLOGY OF ASPARAGINE-LINKED GLYCOSYLATION
    MARK LEHRMAN; Fiscal Year: 2006
    ..Use the unique properties of Lec35 cells and the advantages of the SLO system to identify these glycoconjugates. ..
  5. MOLECULAR BIOLOGY OF ASPARAGINE-LINKED GLYCOSYLATION
    MARK LEHRMAN; Fiscal Year: 1993
    ..In addition, general information will be obtained about interactions between membrane-bound enzymes and lipid substrates, and eventually for sorting or "retention signals" for resident endoplasmic reticulum membrane proteins...
  6. N-Glycosylation And ER Stress
    MARK ANDREW LEHRMAN; Fiscal Year: 2010
    ..Third, we will evaluate a potential host- defense mechanism against certain infectious human viruses. ..