MARK LEHRMANSummaryAffiliation: University of Texas Southwestern Medical Center Country: USA Publications
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Publications
Teaching dolichol-linked oligosaccharides more tricks with alternatives to metabolic radiolabelingMark A Lehrman
Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
Glycobiology 17:75R-85R. 2007..New information revealed by these methods with regard to regulation, genetic disorders, and evolution of the dolichol cycle, as well as caveats of radiolabeling techniques, will be discussed...- Stimulation of N-linked glycosylation and lipid-linked oligosaccharide synthesis by stress responses in metazoan cellsMark A Lehrman
Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 9041, USA
Crit Rev Biochem Mol Biol 41:51-75. 2006..This information will interest readers who study the biological roles of stress responses, the functions of N-linked glycans, and potential strategies for treatment of genetic disorders of N-linked glycosylation... - Rapid activation of glycogen phosphorylase by the endoplasmic reticulum unfolded protein responseArvind Gill
Department of Pharmacology, University of Texas-Southwestern Medical Center, Dallas, Texas 75390-9041, USA
J Biol Chem 277:44747-53. 2002.... 
Translation attenuation by PERK balances ER glycoprotein synthesis with lipid-linked oligosaccharide fluxJie Shang
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
J Cell Biol 176:605-16. 2007..Thus, by sensing ER stress from defective glycosylation, PERK can restore ER homeostasis by balancing polypeptide synthesis with flux through the LLO pathway...- Extension of lipid-linked oligosaccharides is a high-priority aspect of the unfolded protein response: endoplasmic reticulum stress in Type I congenital disorder of glycosylation fibroblastsJie Shang
Department of Pharmacology, Univerity of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9041, USA
Glycobiology 12:307-17. 2002..This suggests that cells stimulate N-glycosylation as part of a first line of defense against ER dysfunction. The broader implications of these results for the biological significance of the UPR are discussed... - Unexpected basis for impaired Glc3Man9GlcNAc2-P-P-dolichol biosynthesis by elevated expression of GlcNAc-1-P transferaseNingguo Gao
Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
Glycobiology 18:125-34. 2008..Since the mammalian GPT gene can undergo spontaneous amplification, the data also indicate a potential basis for forms of pseudo-CDG-If... - Coupling of the dolichol-P-P-oligosaccharide pathway to translation by perturbation-sensitive regulation of the initiating enzyme, GlcNAc-1-P transferaseNingguo Gao
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA
J Biol Chem 277:39425-35. 2002..The implications of these results for the organization of the LLO pathway are discussed... - Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresisNingguo Gao
Department of Pharmacology, UT-Southwestern Medical Center, Dallas, TX, USA
Methods Enzymol 415:3-20. 2006..With FACE, steady-state LLO compositions can be determined quantitatively from cell cultures and animal tissues. We also present FACE methods for analysis of phosphosugars and nucleotide sugars, which are metabolic precursors of LLOs... - Analysis of glycosylation in CDG-Ia fibroblasts by fluorophore-assisted carbohydrate electrophoresis: implications for extracellular glucose and intracellular mannose 6-phosphateNingguo Gao
Department of Pharmacology, University of Texas-Southwestern Medical Center, Dallas, Texas 75390, USA
J Biol Chem 280:17901-9. 2005..The possibilities that M6P may accumulate in hepatocytes and that M6P-stimulated LLO cleavage may account for both hypoglycosylation and the clinical failure of dietary mannose therapy with CDG-Ia patients are discussed... - Characterization of lipid-linked oligosaccharide accumulation in mouse models of Batten diseaseSteve K Cho
Department of Internal Medicine and Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Glycobiology 15:637-48. 2005..3% of the autofluorescent storage material by mass. The accumulation of LLOs is postulated to result from inhibition of late stages of lysosomal degradation of autophagosomes, which may be enriched in these metabolic precursors... - Discordance of UPR signaling by ATF6 and Ire1p-XBP1 with levels of target transcriptsJie Shang
Department of Pharmacology, University of Texas, Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
Biochem Biophys Res Commun 317:390-6. 2004.... - Activation of glycogen phosphorylase with 5-aminoimidazole-4-carboxamide riboside (AICAR). Assessment of glycogen as a precursor of mannosyl residues in glycoconjugatesJie Shang
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9041, USA
J Biol Chem 279:12076-80. 2004..Using this strategy, we show that glycogen can be a significant and regulatable precursor of mannosyl units in lipid-linked oligosaccharides and glycoproteins... - Metformin-stimulated mannose transport in dermal fibroblastsJie Shang
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA
J Biol Chem 279:9703-12. 2004..This suggests that AMP-activated protein kinase may be a regulator of mannose metabolism and implies a therapy for congenital disorders of glycosylation-Ia... - Analyses of dolichol pyrophosphate-linked oligosaccharides in cell cultures and tissues by fluorophore-assisted carbohydrate electrophoresisNingguo Gao
Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas 75390 9041, USA
Glycobiology 12:353-60. 2002..In summary, FACE is a facile, accurate, and sensitive method for LLO analysis, permitting investigations not feasible by metabolic labeling... - Under normoxia, 2-deoxy-D-glucose elicits cell death in select tumor types not by inhibition of glycolysis but by interfering with N-linked glycosylationMetin Kurtoglu
Department of Cell Biologoy and Anatomy, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA
Mol Cancer Ther 6:3049-58. 2007.... - Recycling of dolichyl monophosphate to the cytoplasmic leaflet of the endoplasmic reticulum after the cleavage of dolichyl pyrophosphate on the lumenal monolayerJeffrey S Rush
Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, Kentucky 40536, USA
J Biol Chem 283:4087-93. 2008....
Research Grants
- N-Glycosylation And ER StressMARK LEHRMAN; Fiscal Year: 2009..Third, we will evaluate a potential host- defense mechanism against certain infectious human viruses. ..
- MOLECULAR BIOLOGY OF ASPARAGINE LINKED GLYCOSYLATIONMARK LEHRMAN; Fiscal Year: 2002..V. Measure the selectivity of the existing Lec35 cDNA for mannose-P-dolichol over glucose-P-dolichol (GPD), and isolate a cDNA for a GPD-selective form of Lec35. ..
- LKB1-Independent Metformin ResponseMARK LEHRMAN; Fiscal Year: 2006..This will make possible a future R01 application to determine the role of LIMR in metformin's antihyperglycemic activity. ..
- MOLECULAR BIOLOGY OF ASPARAGINE-LINKED GLYCOSYLATIONMARK LEHRMAN; Fiscal Year: 2006..Use the unique properties of Lec35 cells and the advantages of the SLO system to identify these glycoconjugates. ..
- MOLECULAR BIOLOGY OF ASPARAGINE-LINKED GLYCOSYLATIONMARK LEHRMAN; Fiscal Year: 1993..In addition, general information will be obtained about interactions between membrane-bound enzymes and lipid substrates, and eventually for sorting or "retention signals" for resident endoplasmic reticulum membrane proteins...
- N-Glycosylation And ER StressMARK ANDREW LEHRMAN; Fiscal Year: 2010..Third, we will evaluate a potential host- defense mechanism against certain infectious human viruses. ..