ROBERT IRVING contact LEHRER

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc θ-Defensins: cyclic peptides with endless potential
    Robert I Lehrer
    Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 1688, USA
    J Biol Chem 287:27014-9. 2012
  2. pmc Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia
    Mark T Albrecht
    Division of Biomedical Sciences, University of California, Riverside, California 92521, USA
    Respir Res 3:18. 2002
  3. ncbi request reprint Secretory lipophilins: a tale of two species
    R I Lehrer
    Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA
    Ann N Y Acad Sci 923:59-67. 2000
  4. ncbi request reprint Multispecific myeloid defensins
    Robert I Lehrer
    David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Curr Opin Hematol 14:16-21. 2007
  5. doi request reprint Multivalent binding of carbohydrates by the human alpha-defensin, HD5
    Robert I Lehrer
    David Geffen School of Medicine at University of California at Los Angeles, 90095, USA
    J Immunol 183:480-90. 2009
  6. pmc Human alpha-defensins inhibit hemolysis mediated by cholesterol-dependent cytolysins
    Robert I Lehrer
    Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Infect Immun 77:4028-40. 2009
  7. ncbi request reprint Primate defensins
    Robert I Lehrer
    Department of Medicine and Molecular Biology Institute, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90095, USA
    Nat Rev Microbiol 2:727-38. 2004
  8. doi request reprint α-Defensins in human innate immunity
    Robert I Lehrer
    Department of Medicine and Molecular Biology Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1688, USA
    Immunol Rev 245:84-112. 2012
  9. ncbi request reprint Defensins of vertebrate animals
    Robert I Lehrer
    Department of Medicine Room CHS 37 062, and Molecular Biology Institute University of California at Los Angeles UCLA Center for the Health Sciences, Los Angeles, CA 90095, USA
    Curr Opin Immunol 14:96-102. 2002
  10. ncbi request reprint Cathelicidins: a family of endogenous antimicrobial peptides
    Robert I Lehrer
    Department of Medicine, Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California, USA
    Curr Opin Hematol 9:18-22. 2002

Research Grants

Collaborators

Detail Information

Publications66

  1. pmc θ-Defensins: cyclic peptides with endless potential
    Robert I Lehrer
    Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 1688, USA
    J Biol Chem 287:27014-9. 2012
    ..The small size, unique structure, and multiple host defense activities of θ-defensins make them intriguing potential therapeutic agents...
  2. pmc Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia
    Mark T Albrecht
    Division of Biomedical Sciences, University of California, Riverside, California 92521, USA
    Respir Res 3:18. 2002
    ..aeruginosa. The present study evaluates the correlation between protegrin-1 (PG-1) sensitivity/resistance and protegrin binding in P. aeruginosa and B. cepacia...
  3. ncbi request reprint Secretory lipophilins: a tale of two species
    R I Lehrer
    Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA
    Ann N Y Acad Sci 923:59-67. 2000
    ..Although the functions of secretory lipophilins are imperfectly understood, their abundance in glandular secretions and in hormone-responsive tissues suggests that they deserve considerably more attention than they have received to date...
  4. ncbi request reprint Multispecific myeloid defensins
    Robert I Lehrer
    David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Curr Opin Hematol 14:16-21. 2007
    ..Monocytes and macrophages generally lack defensins, but they release messengers that induce the synthesis of beta-defensins in epithelial cells...
  5. doi request reprint Multivalent binding of carbohydrates by the human alpha-defensin, HD5
    Robert I Lehrer
    David Geffen School of Medicine at University of California at Los Angeles, 90095, USA
    J Immunol 183:480-90. 2009
    ..Self-association and multivalent binding may play integral roles in the ability of HD5 to protect against infections caused by viruses and other infectious agents...
  6. pmc Human alpha-defensins inhibit hemolysis mediated by cholesterol-dependent cytolysins
    Robert I Lehrer
    Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Infect Immun 77:4028-40. 2009
    ..An "ABCDE mechanism" that can account for the ability of HNP-1 to HNP-3 to inhibit so many different exotoxins is proposed...
  7. ncbi request reprint Primate defensins
    Robert I Lehrer
    Department of Medicine and Molecular Biology Institute, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90095, USA
    Nat Rev Microbiol 2:727-38. 2004
    ..Here, their discovery and recent advances in understanding their properties and functions are described...
  8. doi request reprint α-Defensins in human innate immunity
    Robert I Lehrer
    Department of Medicine and Molecular Biology Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1688, USA
    Immunol Rev 245:84-112. 2012
    ..The review ends with a decalogue that may assist researchers or students interested in α-defensins and related aspects of neutrophil function...
  9. ncbi request reprint Defensins of vertebrate animals
    Robert I Lehrer
    Department of Medicine Room CHS 37 062, and Molecular Biology Institute University of California at Los Angeles UCLA Center for the Health Sciences, Los Angeles, CA 90095, USA
    Curr Opin Immunol 14:96-102. 2002
    ..There is now convincing evidence that defensins augment adaptive immune responses...
  10. ncbi request reprint Cathelicidins: a family of endogenous antimicrobial peptides
    Robert I Lehrer
    Department of Medicine, Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California, USA
    Curr Opin Hematol 9:18-22. 2002
    ..Porcine protegrins are in stage III clinical trials to prevent oral mucositis caused by radiation or chemo-therapy...
  11. ncbi request reprint In defense of skin
    Robert I Lehrer
    Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Invest Dermatol 125:viii-ix; discussion x-xi. 2005
  12. ncbi request reprint Retrocyclin, an antiretroviral theta-defensin, is a lectin
    Wei Wang
    Department of Medicine, University of California, Los Angeles, CA 90095, USA
    J Immunol 170:4708-16. 2003
    ..Retrocyclin's ability to recognize and bind carbohydrate-containing surface molecules is integrally related to its ability to protect cells from HIV-1 infection...
  13. pmc Anti-inflammatory apoA-I-mimetic peptides bind oxidized lipids with much higher affinity than human apoA-I
    Brian J Van Lenten
    Department of Medicine David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095 1679, USA
    J Lipid Res 49:2302-11. 2008
    ..The extraordinary ability of 4F to bind pro-inflammatory oxidized lipids probably accounts for its remarkable anti-inflammatory properties...
  14. pmc A novel method for oral delivery of apolipoprotein mimetic peptides synthesized from all L-amino acids
    Mohamad Navab
    Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1679, USA
    J Lipid Res 50:1538-47. 2009
    ..Niclosamide itself did not inhibit trypsin activity. The combination of niclosamide with apolipoprotein mimetic peptides appears to be a promising method for oral delivery of these peptides...
  15. pmc Oxpholipin 11D: an anti-inflammatory peptide that binds cholesterol and oxidized phospholipids
    Piotr Ruchala
    Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 5:e10181. 2010
    ..This domain is usually flanked N-terminally by arginine and C-terminally by valine. We used this 14-residue sequence as a template to create a small library of peptides that bind cholesterol and other lipids...
  16. pmc Theta defensins protect cells from infection by herpes simplex virus by inhibiting viral adhesion and entry
    Bushra Yasin
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1731, USA
    J Virol 78:5147-56. 2004
    ....
  17. pmc Retrocyclin: a primate peptide that protects cells from infection by T- and M-tropic strains of HIV-1
    Alexander M Cole
    Department of Medicine, AIDS Institute, University of California School of Medicine, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 99:1813-8. 2002
    ..These findings suggest that retrocyclin interferes with an early stage of HIV-1 infection and that retrocyclin-like agents might be useful topical agents to prevent sexually acquired HIV-1 infections...
  18. ncbi request reprint Potassium release, a useful tool for studying antimicrobial peptides
    Dmitri S Orlov
    Department of Medicine, UCLA Center for Health Sciences, 10833 LeConte Avenue, Los Angeles, CA 90095, USA
    J Microbiol Methods 49:325-8. 2002
    ..We used an ion-selective electrode to measure potassium release from bacteria treated with antimicrobial peptides. This broadly applicable and simple technique for assessing membrane integrity deserves greater use...
  19. ncbi request reprint Minidefensins: antimicrobial peptides with activity against HIV-1
    Alexander M Cole
    David Geffen School of Medicine at UCLA, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Los Angeles, CA 90095, USA
    Curr Pharm Des 9:1463-73. 2003
    ..In addition to describing critical elements of their structure and unusual mode of formation, we will venture some predictions about using theta-defensins as antiviral agents...
  20. ncbi request reprint Activity of alpha- and theta-defensins against primary isolates of HIV-1
    Wei Wang
    Department of Medicine, David Geffen School of Medicine at University of California Los Angeles, 10833 LeConte Avenue, Los Angeles, CA 90095, USA
    J Immunol 173:515-20. 2004
    ....
  21. doi request reprint Interactions of alpha-, beta-, and theta-defensins with influenza A virus and surfactant protein D
    Mona Doss
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    J Immunol 182:7878-87. 2009
    ..Given their strong neutralizing activity and compatibility with SP-D, RCs may provide attractive prototypes for designing therapeutics that can prevent or treat respiratory infections caused by IAV...
  22. ncbi request reprint Characterization of a lipophilin in rabbit tears
    Ben J Glasgow
    Department of Pathology and Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, California, USA
    Adv Exp Med Biol 506:573-80. 2002
  23. pmc Retrocyclins kill bacilli and germinating spores of Bacillus anthracis and inactivate anthrax lethal toxin
    Wei Wang
    Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, 10833 LeConte Avenue, Los Angeles, CA 90095, USA
    J Biol Chem 281:32755-64. 2006
    ..Overall, these findings suggest that theta-defensins provide molecular templates that could be used to create novel agents effective against B. anthracis and its toxins...
  24. ncbi request reprint Examination of Chlamydia trachomatis infection in environments mimicking normal and abnormal vaginal pH
    Bushra Yasin
    Department of Pathology and Laboratory Medicine, UCLA School of Medicine, Los Angeles, California 90095 1731, USA
    Sex Transm Dis 29:514-9. 2002
    ....
  25. ncbi request reprint Paradoxical effects of two theta-defensins on HIV type 1 infection
    Qiuwei Wang
    UCLA School of Dentistry, and Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, CA 90095, USA
    AIDS Res Hum Retroviruses 23:508-14. 2007
    ..The ability of RC-111 to enhance HIV-1 infection might prove useful in developing peptides that can enhance gene delivery by HIV-based lentiviral vectors...
  26. ncbi request reprint Immunolocalization of clavanins in Styela clava hemocytes
    Lorenzo P Menzel
    Department of Medicine, UCLA School of Medicine, CHS 37 062, 10833 LeConte Avenue, Los Angeles, CA 90095 1690, USA
    Dev Comp Immunol 26:505-15. 2002
    ..Orange G and the century-old trichrome stain may provide simple screening tools for identifying cells that contain large amounts of antimicrobial peptides in mixed hemocyte populations...
  27. ncbi request reprint Evolution of primate theta-defensins: a serpentine path to a sweet tooth
    Tung X Nguyen
    Department of Medicine, UCLA Center for the Health Sciences, Room CHS 37 062, 10833 LeConte Avenue, Los Angeles, CA 90095, USA
    Peptides 24:1647-54. 2003
    ..It is possible (but may be difficult to prove) that this mutation rendered our species more susceptible to infection by HIV-1...
  28. pmc Humanized theta-defensins (retrocyclins) enhance macrophage performance and protect mice from experimental anthrax infections
    S Welkos
    Bacteriology Division, U S Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA
    Antimicrob Agents Chemother 55:4238-50. 2011
    ..Overall, these data demonstrate that retrocyclins are effective in vivo against experimental murine anthrax infections and suggest that enhanced macrophage function contributes to this property...
  29. ncbi request reprint Paradise lost and paradigm found
    Robert I Lehrer
    Nat Immunol 5:775-6. 2004
  30. ncbi request reprint HIV-1 adapts to a retrocyclin with cationic amino acid substitutions that reduce fusion efficiency of gp41
    Amy L Cole
    Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, FL 32816, USA
    J Immunol 176:6900-5. 2006
    ..These findings also suggest that interactions between theta-defensins and gp41 may contribute to the ability of these cyclic minidefensins to prevent HIV-1 entry into target cells...
  31. ncbi request reprint Insertion selectivity of antimicrobial peptide protegrin-1 into lipid monolayers: effect of head group electrostatics and tail group packing
    Yuji Ishitsuka
    Department of Chemistry, The Institute for Biophysical Dynamics and the James Franck Institute, The University of Chicago, Chicago, IL 60637, USA
    Biochim Biophys Acta 1758:1450-60. 2006
    ..However, the presence of PG-1 can still affect lipid morphology even with no observed PG-1 insertion. These results show the important role that lipid composition of the cell membrane plays in the activity of AMPs...
  32. pmc Membrane-dependent oligomeric structure and pore formation of a beta-hairpin antimicrobial peptide in lipid bilayers from solid-state NMR
    Rajeswari Mani
    Department of Chemistry, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 103:16242-7. 2006
    ..Thus, the membrane composition plays a decisive role in defining the assembly and insertion of PG-1. The different oligomeric structures of PG-1 help to explain its greater toxicity for bacteria than for eukaryotic cells...
  33. ncbi request reprint Headgroup structure and fatty acid chain length of the acidic phospholipids modulate the interaction of membrane mimetic vesicles with the antimicrobial peptide protegrin-1
    Weiguo Jing
    Institute of Biophysics and X ray Structure Research, Austrian Academy of Sciences, Graz
    J Pept Sci 11:735-43. 2005
    ....
  34. pmc The retrocyclin analogue RC-101 prevents human immunodeficiency virus type 1 infection of a model human cervicovaginal tissue construct
    Amy L Cole
    Department of Molecular Biology and Microbiology, Biomolecular Science Center, Burnett College of Biomedical Sciences at University of Central Florida, Orlando, FL 32816, USA
    Immunology 121:140-5. 2007
    ..Collectively, these data support the potential development of RC-101 as a topical microbicide to prevent HIV-1 infection and transmission...
  35. ncbi request reprint Carbohydrate-binding molecules inhibit viral fusion and entry by crosslinking membrane glycoproteins
    Eugenia Leikina
    Section on Membrane Biology, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892 1855, USA
    Nat Immunol 6:995-1001. 2005
    ..This general mechanism might explain the broad-spectrum antiviral activity of many multivalent lectins of the innate immune system...
  36. ncbi request reprint Solid-state NMR investigations of peptide-lipid interaction and orientation of a beta-sheet antimicrobial peptide, protegrin
    Satoru Yamaguchi
    Department of Chemistry, Iowa State University, Ames, IA 50011, USA
    Biochemistry 41:9852-62. 2002
    ..This is the first time that the orientation of a disulfide-stabilized beta-sheet membrane peptide has been determined by solid-state NMR...
  37. ncbi request reprint Retrocyclin RC-101 overcomes cationic mutations on the heptad repeat 2 region of HIV-1 gp41
    Christopher A Fuhrman
    Molecular Biology and Microbiology, Biomolecular Science Center, Burnett College of Biomedical Sciences at University of Central Florida, Orlando, FL 32816, USA
    FEBS J 274:6477-87. 2007
    ....
  38. ncbi request reprint RC-101, a retrocyclin-1 analogue with enhanced activity against primary HIV type 1 isolates
    Sherry M Owen
    HIV Immunology and Diagnostic Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    AIDS Res Hum Retroviruses 20:1157-65. 2004
    ..The performance of RC-101 suggests that additional "engineering" could further enhance the antiviral properties of theta-defensins...
  39. ncbi request reprint Retrocyclin-2: structural analysis of a potent anti-HIV theta-defensin
    Norelle L Daly
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane QLD 4072, Australia
    Biochemistry 46:9920-8. 2007
    ..The ability to self-associate may contribute to the high-affinity binding of retrocyclins for glycoproteins by increasing the valency and enhancing the ability of retrocyclins to cross-link cell surface glycoproteins...
  40. pmc Interaction of antimicrobial peptide protegrin with biomembranes
    David Gidalevitz
    Department of Chemical Engineering, University of Leeds, United Kingdom
    Proc Natl Acad Sci U S A 100:6302-7. 2003
    ....
  41. ncbi request reprint The theta-defensin, retrocyclin, inhibits HIV-1 entry
    Carsten Munk
    Infectious Disease Laboratory, Salk Institute for Biological Studies, San Diego, CA 92037, USA
    AIDS Res Hum Retroviruses 19:875-81. 2003
    ..In the aggregate, these results suggest that retrocyclin blocks an entry step in HIV-1 replication. Retrocyclin represents a new class of small molecule HIV-1 entry inhibitors...
  42. ncbi request reprint Evaluation of a theta-defensin in a Murine model of herpes simplex virus type 1 keratitis
    Curtis R Brandt
    Department of Ophthalmology and Visual Science, University of Wisconsin, Madison, WI 53706, USA
    Invest Ophthalmol Vis Sci 48:5118-24. 2007
    ..To test the activity of a synthetic theta-defensin, retrocyclin (RC)-2, in a murine herpes simplex virus (HSV)-1 keratitis model...
  43. doi request reprint Effects of guanidinium-phosphate hydrogen bonding on the membrane-bound structure and activity of an arginine-rich membrane peptide from solid-state NMR spectroscopy
    Ming Tang
    Department of Chemistry, Iowa State University, Ames, IA 50011, USA
    Angew Chem Int Ed Engl 47:3202-5. 2008
  44. doi request reprint Dual mechanism of bacterial lethality for a cationic sequence-random copolymer that mimics host-defense antimicrobial peptides
    Raquel F Epand
    Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada
    J Mol Biol 379:38-50. 2008
    ..We propose that this polymer has two mechanisms of antibacterial action, one predominating at low concentrations of polymer and the other predominating at high concentrations...
  45. pmc Correlation between simulated physicochemical properties and hemolycity of protegrin-like antimicrobial peptides: predicting experimental toxicity
    Allison A Langham
    Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455, USA
    Peptides 29:1085-93. 2008
    ..The developed QSARs provide insight into the mechanism of cytotoxic action of antimicrobial peptides. In a subsequent blind test, the QSAR correctly ranked four of five protegrin analogues newly synthesized and tested for toxicity...
  46. ncbi request reprint SMAP-29 has two LPS-binding sites and a central hinge
    Brian F Tack
    Department of Microbiology, College of Medicine NMR Facility and Biochemistry, University of Iowa, IA, USA
    Eur J Biochem 269:1181-9. 2002
    ..5 microm, and bound LPS with positive cooperativity. We conclude that the presence of multiple binding sites that function cooperatively allow peptides such as SMAP-29 and CAP-18 to bind LPS with high affinity...
  47. ncbi request reprint Susceptibility of Treponema pallidum to host-derived antimicrobial peptides
    David L Cox
    Sexually Transmitted Infections Branch, Division of AIDS, STD and TB Laboratory Research, Center for HIV and STD Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Peptides 24:1741-6. 2003
    ..pallidum. Infectivity of T. pallidum in a rabbit model could effectively be blocked with the synthetic truncated LL-37-derived peptide WS22-N-amide...
  48. ncbi request reprint Impact of single-residue mutations on the structure and function of ovispirin/novispirin antimicrobial peptides
    Monali V Sawai
    Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242, USA
    Protein Eng 15:225-32. 2002
    ..These findings show that simple structural modifications can 'fine-tune' an antimicrobial peptide to minimize unwanted cytotoxicity while retaining its desired activity...
  49. ncbi request reprint Avian beta-defensin nomenclature: a community proposed update
    David J Lynn
    Immunol Lett 110:86-9. 2007
  50. ncbi request reprint The innate immune system: a repository for future drugs?
    Suryaprakash Sambhara
    Expert Rev Anti Infect Ther 5:1-5. 2007
  51. pmc Solid-state NMR investigation of the depth of insertion of protegrin-1 in lipid bilayers using paramagnetic Mn2+
    Jarrod J Buffy
    Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA
    Biophys J 85:2363-73. 2003
    ..The depth sensitivity of this Mn(2+) dephasing technique is tunable with the Mn(2+) concentration to focus on different regions of the lipid bilayer...
  52. ncbi request reprint Interaction of antimicrobial peptides with lipopolysaccharides
    Lai Ding
    Department of Physics and Astronomy, Rice University, Houston, Texas 77251 1892, USA
    Biochemistry 42:12251-9. 2003
    ..At peptide concentrations comparable to the physiological conditions, both peptides penetrate transmembrane in LPS bilayers. The results imply that antimicrobial peptides readily penetrate the LPS monolayer of the outer membrane...
  53. ncbi request reprint Immobilization and aggregation of the antimicrobial peptide protegrin-1 in lipid bilayers investigated by solid-state NMR
    Jarrod J Buffy
    Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA
    Biochemistry 42:13725-34. 2003
    ..Thus, solid-state NMR provides a wide range of information about the molecular details of membrane peptide immobilization and aggregation in lipid bilayers...
  54. ncbi request reprint Direct comparison of membrane interactions of model peptides composed of only Leu and Lys residues
    Raquel F Epand
    Department of Biochemistry, McMaster University, Hamilton, ON L8N 3Z5, Canada
    Biopolymers 71:2-16. 2003
    ....
  55. ncbi request reprint Solid-state NMR investigation of the selective disruption of lipid membranes by protegrin-1
    Rajeswari Mani
    Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA
    Biochemistry 43:13839-48. 2004
    ..The observed selective interaction of PG-1 with different lipid membranes is consistent with its biological function and may be caused by its strong cationic and amphipathic structure...
  56. ncbi request reprint Retrocyclins: using past as prologue
    Alexander M Cole
    Department of Molecular Biology, Biomolecular Science Center, University of Central Florida, Orlando, FL 32816 2364, USA
    Curr Protein Pept Sci 5:373-81. 2004
    ..This unusual combination makes Theta-defensins intriguing molecular prototypes that could be used to design novel carbohydrate-binding or antiviral agents...
  57. ncbi request reprint Human neutrophil alpha-defensin 4 inhibits HIV-1 infection in vitro
    Zhibin Wu
    Institute of Human Virology, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, MD 21201, USA
    FEBS Lett 579:162-6. 2005
    ..Our finding identifies an anti-HIV-1 property of HNP4 and may have implications in the development of new antiviral agents for AIDS therapy...
  58. ncbi request reprint Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus
    Per H Mygind
    Novozymes A S, 2880 Bagsvaerd, Denmark
    Nature 437:975-80. 2005
    ..These findings identify fungi as a novel source of antimicrobial defensins, and show the therapeutic potential of plectasin. They also suggest that the defensins of insects, molluscs and fungi arose from a common ancestral gene...
  59. ncbi request reprint Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
    Minnie John
    Department of Pediatrics, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Infect Dis 192:1731-40. 2005
    ..In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity...
  60. pmc Orientation of a beta-hairpin antimicrobial peptide in lipid bilayers from two-dimensional dipolar chemical-shift correlation NMR
    Ming Tang
    Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA
    Biophys J 90:3616-24. 2006
    ..These structural results provide insights into the mechanism of action of this beta-hairpin antimicrobial peptide...
  61. ncbi request reprint Theta-defensins prevent HIV-1 Env-mediated fusion by binding gp41 and blocking 6-helix bundle formation
    Stephen A Gallo
    Center for Cancer Research Nanobiology Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 281:18787-92. 2006
    ..This mode of action, although novel for an innate effector molecule, resembles the mechanism of peptidic entry inhibitors based on portions of the gp41 sequence...
  62. ncbi request reprint Solid-state NMR investigation of the selective perturbation of lipid bilayers by the cyclic antimicrobial peptide RTD-1
    Jarrod J Buffy
    Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA
    Biochemistry 43:9800-12. 2004
    ..These structural and dynamical features of RTD-1 differ significantly from those of PG-1, a related beta-hairpin antimicrobial peptide...
  63. ncbi request reprint Mechanism of supported membrane disruption by antimicrobial peptide protegrin-1
    Kin Lok H Lam
    Department of Physics, The Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Illinois 60637, USA
    J Phys Chem B 110:21282-6. 2006
    ..This first direct visualization of these membrane structural transformations helps elucidate the PG-1-induced membrane disruption mechanism...
  64. ncbi request reprint Human alpha- and beta-defensins block multiple steps in herpes simplex virus infection
    Ehsan Hazrati
    Department of Pediatrics, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Immunol 177:8658-66. 2006
    ..These findings demonstrate that HDs act at multiple steps in the HSV life cycle and support the development of defensins or defensin-like peptides as microbicides...
  65. ncbi request reprint Membrane-disruptive abilities of beta-hairpin antimicrobial peptides correlate with conformation and activity: a 31P and 1H NMR study
    Rajeswari Mani
    Department of Chemistry, Iowa State University, Ames, IA 50011, USA
    Biochim Biophys Acta 1716:11-8. 2005
    ....
  66. pmc Activity of novispirin G10 against Pseudomonas aeruginosa in vitro and in infected burns
    Lars Steinstraesser
    Department of Surgery, University of Michigan, Ann Arbor, Michigan, USA
    Antimicrob Agents Chemother 46:1837-44. 2002
    ..Antimicrobial peptides such as novispirin G10 may provide a useful alternative or adjunct to standard antibiotic agents in treating burns or other wound infections...

Research Grants19

  1. Retrocyclins: Cyclic mini-defensins that inactivate anthrax toxins
    Robert Lehrer; Fiscal Year: 2009
    ..anthracis spores in mouse models as per the revised Specific Aims. All remaining Aims can be addressed in a two-year time period. PHS ..
  2. Retrocyclin reinforcement of pulmonary defenses against viral aerosols
    Robert Lehrer; Fiscal Year: 2007
    ....
  3. Theta-defensins Novel HIV-1 Uptake Inhibitors
    Robert Lehrer; Fiscal Year: 2007
    ..Specific Aim 2 will be realized by a combination of microchemical techniques, including surface plasmon resonance, tandem mass spectrometry, and state-of-the-art lectinomic and peptidomic chemistry. ..
  4. Biacore 3000 SPR Instrument
    Robert Lehrer; Fiscal Year: 2004
    ..These include the development of new agents and approaches to prevent and treat infections caused by HIV-1 and other viral pathogens, and fundamental issues related to cell targeting and survival. ..
  5. ALPHA HELICAL CATHELICIDINS AND HOST DEFENSE
    Robert Lehrer; Fiscal Year: 2003
    ..The experiments will expand our knowledge about these important peptides, and should provide the information needed to develop them as antibiotics for topical bronchopulmonary use in cystic fibrosis. ..
  6. DEFENSINS--ANTIMICROBIAL PEPTIDES OF HUMAN NEUTROPHILS
    Robert Lehrer; Fiscal Year: 2002
    ..The work with murine neutrophils, whose content of endogenous antimicrobial polypeptides differs greatly from those of humans, will provide essential information relevant to the interpretation of murine models of human infection. ..
  7. ALTRUINS, NATURAL ANTIBIOTICS OF THE MALE GENITAL TRACT
    Robert Lehrer; Fiscal Year: 2001
    ..S. are sexually transmitted. According to CDC estimates, STDs other than AIDS add 10 billion dollars to the nation's annual health care I costs. The proposed studies could lead to novel approaches for STD prophylaxis. ..
  8. STRUCTURE/FUNCTIONS OF HUMAN TEAR LIPOPHILIN
    Robert Lehrer; Fiscal Year: 2001
    ..The long-term objectives for this research are to learn how lipophilin contributes to corneal and conjuctival health, and to explore its potential for creating new diagnostic and therapeutic modalities. ..
  9. Retrocyclins: Cyclic mini-defensins that inactivate anthrax toxins
    ROBERT IRVING contact LEHRER; Fiscal Year: 2010
    ..anthracis spores in mouse models as per the revised Specific Aims. All remaining Aims can be addressed in a two-year time period. PHS ..