Sang Eun Lee

Summary

Affiliation: University of Texas Health Science Center
Country: USA

Publications

  1. ncbi request reprint Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase
    Moreshwar B Vaze
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Mol Cell 10:373-85. 2002
  2. doi request reprint Saccharomyces cerevisiae ATM orthologue suppresses break-induced chromosome translocations
    Kihoon Lee
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Nature 454:543-6. 2008
  3. pmc Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining
    Kihoon Lee
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Genetics 176:2003-14. 2007
  4. pmc Faithful after break-up: suppression of chromosomal translocations
    Sang Eun Lee
    Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78245, USA
    Cell Mol Life Sci 66:3149-60. 2009
  5. pmc RSC facilitates Rad59-dependent homologous recombination between sister chromatids by promoting cohesin loading at DNA double-strand breaks
    Ji Hyun Oum
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell Biol 31:3924-37. 2011
  6. pmc Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks
    Eun Yong Shim
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
    EMBO J 29:3370-80. 2010
  7. pmc Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates
    Fuyang Li
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell 30:325-35. 2008
  8. pmc The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks
    Eun Yong Shim
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell Biol 25:3934-44. 2005
  9. pmc RSC mobilizes nucleosomes to improve accessibility of repair machinery to the damaged chromatin
    Eun Yong Shim
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell Biol 27:1602-13. 2007
  10. pmc Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences
    Jia Lin Ma
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Mol Cell Biol 23:8820-8. 2003

Research Grants

  1. Mechanisms of Error Prone Repair of DNA Breaks
    Sang Eun Lee; Fiscal Year: 2010
  2. Etiology of Chromosome Translocations
    Sang Eun Lee; Fiscal Year: 2010
  3. Mechanisms of Error Prone Repair of DNA Breaks
    Sang Eun Lee; Fiscal Year: 2009
  4. Mechanisms of Error Prone Repair of DNA Breaks
    Sang Eun Lee; Fiscal Year: 2007
  5. Mechanisms of Nonhomologous Repair of Damaged DNA
    Sang Eun Lee; Fiscal Year: 2005
  6. Etiology of Chromosome Translocations
    Sang Eun Lee; Fiscal Year: 2009

Collaborators

  • PATRICK M SUNG
  • Yu Zhang
  • Grzegorz Ira
  • Xuewen Pan
  • Tanya T Paull
  • J E Haber
  • Giordano Liberi
  • A E Tomkinson
  • JOHN HJ PETRINI
  • Eun Yong Shim
  • Ji Hyun Oum
  • Kihoon Lee
  • Zhu Zhu
  • Diana D Villarreal
  • Anna Malkova
  • Jia Lin Ma
  • Woo Hyun Chung
  • Moreshwar B Vaze
  • Fuyang Li
  • Soma Banerjee
  • Yvonne Yanez
  • Yves Corda
  • Ayelet Arbel-Eden
  • Christophe Leroy
  • Angela Deem
  • Sreejith Ramakrishnan
  • YoungHo Kwon
  • Jae Hoon Ji
  • Amy Sid
  • Changhyun Seong
  • Matthew L Nicolette
  • Melody Davis
  • Kyungjae Myung
  • Akira Motegi
  • Nilabja Sikdar
  • Ji Young Hwang
  • Junchao Dong
  • Stephanie Smith
  • Hung Jiun Liaw
  • Jef D Boeke
  • Soo Jin Hong
  • Sylvine Guillot
  • Julie Sollier
  • André Walther
  • Vincent Geli
  • Francoise Ochsenbein
  • Raphael Guerois
  • Marie Claude Marsolier-Kergoat
  • Françoise Ochsenbien
  • Eun Mi Kim
  • Achille Pellicioli
  • Marco Foiani

Detail Information

Publications19

  1. ncbi request reprint Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase
    Moreshwar B Vaze
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Mol Cell 10:373-85. 2002
    ..Permanent preanaphase arrest of srs2Delta cells is reversed by the addition of caffeine after cells have arrested. Thus, in addition to its roles in recombination, Srs2p appears to be needed to turn off the DNA damage checkpoint...
  2. doi request reprint Saccharomyces cerevisiae ATM orthologue suppresses break-induced chromosome translocations
    Kihoon Lee
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Nature 454:543-6. 2008
    ....
  3. pmc Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining
    Kihoon Lee
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Genetics 176:2003-14. 2007
    ..Sae2 and Tel1 promote MMEJ but inhibit NHEJ, likely by regulating Mre11-dependent ssDNA accumulation at DNA break. Our data support the role of Sae2 and Tel1 in MMEJ and genome integrity...
  4. pmc Faithful after break-up: suppression of chromosomal translocations
    Sang Eun Lee
    Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78245, USA
    Cell Mol Life Sci 66:3149-60. 2009
    ..These pathways select proper homologous templates or broken DNA ends for the faithful repair of DNA breaks to avoid undesirable chromosomal translocations...
  5. pmc RSC facilitates Rad59-dependent homologous recombination between sister chromatids by promoting cohesin loading at DNA double-strand breaks
    Ji Hyun Oum
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell Biol 31:3924-37. 2011
    ..This study provides molecular insights into how chromatin remodeling contributes to DNA repair and maintenance of chromatin fidelity in the face of DNA damage...
  6. pmc Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks
    Eun Yong Shim
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
    EMBO J 29:3370-80. 2010
    ..However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation...
  7. pmc Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates
    Fuyang Li
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell 30:325-35. 2008
    ..Deletion of SAW1 abolished association of Rad1 at SSA intermediates in vivo. We propose that Saw1 targets Rad1/Rad10 to Rad52-coated recombination intermediates...
  8. pmc The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks
    Eun Yong Shim
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell Biol 25:3934-44. 2005
    ..The interaction of Rsc1p with Mre11p appears to be vital for survival from genotoxic stress. These results suggest that chromatin remodeling by RSC is important for NHEJ...
  9. pmc RSC mobilizes nucleosomes to improve accessibility of repair machinery to the damaged chromatin
    Eun Yong Shim
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mol Cell Biol 27:1602-13. 2007
    ..We propose that RSC-mediated chromatin remodeling at the DSB prepares chromatin to allow repair machinery to access the break and is vital for efficient DSB repair...
  10. pmc Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences
    Jia Lin Ma
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Mol Cell Biol 23:8820-8. 2003
    ..The increased gamma ray sensitivity of rad1Delta rad52Delta yku70Delta strains compared to rad52Delta yku70Delta strains suggests that MMEJ also contributes to the repair of DSBs induced by ionizing radiation...
  11. ncbi request reprint Role of Dnl4-Lif1 in nonhomologous end-joining repair complex assembly and suppression of homologous recombination
    Yu Zhang
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, Texas 78245, USA
    Nat Struct Mol Biol 14:639-46. 2007
    ..Therefore, Dnl4-Lif1 plays an important part in determining repair pathway choice by participating at an early stage of DSB engagement in addition to providing the DNA ligase activity that completes NHEJ...
  12. pmc Regulation of repair choice: Cdk1 suppresses recruitment of end joining factors at DNA breaks
    Yu Zhang
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 78245, United States
    DNA Repair (Amst) 8:1235-41. 2009
    ..Expression of excess Ku can partially offset the inhibition of end joining at G(2). The results suggest that regulation of Ku/Dnl4-Lif1 affinity for DNA ends may contribute to the cell cycle-dependent modulation of NHEJ efficiency...
  13. pmc Microhomology directs diverse DNA break repair pathways and chromosomal translocations
    Diana D Villarreal
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America
    PLoS Genet 8:e1003026. 2012
    ..MHMR catalyzes chromosomal translocation almost as efficiently as intra-chromosomal repair. The results suggest that the intrinsic annealing propensity between microhomology sequences efficiently leads to chromosomal rearrangements...
  14. pmc Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends
    Zhu Zhu
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Cell 134:981-94. 2008
    ..These results provide important insights into the early steps of DSB repair in eukaryotes...
  15. pmc Inactivation of Ku-mediated end joining suppresses mec1Delta lethality by depleting the ribonucleotide reductase inhibitor Sml1 through a pathway controlled by Tel1 kinase and the Mre11 complex
    Yves Corda
    Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
    Mol Cell Biol 25:10652-64. 2005
    ..We further report that this Mec1-independent pathway converges with the Rad53/Dun1-regulated checkpoint kinase cascade and leads to the degradation of the ribonucleotide reductase inhibitor Sml1...
  16. pmc Yeast Rad52 and Rad51 recombination proteins define a second pathway of DNA damage assessment in response to a single double-strand break
    Sang Eun Lee
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Mol Cell Biol 23:8913-23. 2003
    ..We suggest that monitoring of the extent of DNA damage depends on independent binding of RPA and Rad52p to ssDNA, with Rad52p's activity modulated by Rad51p whereas RPA's action depends on Tid1p...
  17. ncbi request reprint PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break
    Christophe Leroy
    Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette, Cedex, France
    Mol Cell 11:827-35. 2003
    ..In vivo and in vitro evidence suggests that phosphorylated forms of Ptc2 and Ptc3 specifically bind to the Rad53 FHA1 domain and inactivate Rad53-dependent pathways during adaptation and recovery by dephosphorylating Rad53...
  18. ncbi request reprint Complementation between N-terminal Saccharomyces cerevisiae mre11 alleles in DNA repair and telomere length maintenance
    Sang Eun Lee
    Rosenstiel Center and Department of Biology, Brandeis University, MS029 Waltham, MA 02454 9110, USA
    DNA Repair (Amst) 1:27-40. 2002
    ..We propose that at least two separate activities associated with the N-terminus of Mre11p are required for its mitotic function...
  19. pmc Mph1p promotes gross chromosomal rearrangement through partial inhibition of homologous recombination
    Soma Banerjee
    Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 181:1083-93. 2008
    ..Furthermore, spontaneous RPA foci at DSBs are destabilized by the mph1Delta mutation. Therefore, Mph1p promotes GCR formation by partially suppressing HR, likely through its interaction with RPA...

Research Grants13

  1. Mechanisms of Error Prone Repair of DNA Breaks
    Sang Eun Lee; Fiscal Year: 2010
    ..The information gleaned from the proposal will also provide a logical framework as to how cells tolerate cancer therapeutic agent-induced DNA lesions and shed lights on the prevention and improved therapeutic intervention of cancers. ..
  2. Etiology of Chromosome Translocations
    Sang Eun Lee; Fiscal Year: 2010
    ..This application will identify and characterize damage- surveillance and other mechanisms that suppress chromosome translocation to understand the defects in blood cancers and develop novel preventive and/or therapeutic strategies. ..
  3. Mechanisms of Error Prone Repair of DNA Breaks
    Sang Eun Lee; Fiscal Year: 2009
    ..The information gleaned from the proposal will also provide a logical framework as to how cells tolerate cancer therapeutic agent-induced DNA lesions and shed lights on the prevention and improved therapeutic intervention of cancers. ..
  4. Mechanisms of Error Prone Repair of DNA Breaks
    Sang Eun Lee; Fiscal Year: 2007
    ..Since the components of DSB repair are conserved from yeast to humans, the insights garnered from our research will be valuable for dissecting the equivalent process in human cells and will be of relevance to public health. ..
  5. Mechanisms of Nonhomologous Repair of Damaged DNA
    Sang Eun Lee; Fiscal Year: 2005
    ..Furthermore, since DSB repair by NHEJ is remarkably conserved from yeast to humans, these studies will help to dissect the similar pathways in humans. ..
  6. Etiology of Chromosome Translocations
    Sang Eun Lee; Fiscal Year: 2009
    ..This application will identify and characterize damage- surveillance and other mechanisms that suppress chromosome translocation to understand the defects in blood cancers and develop novel preventive and/or therapeutic strategies. ..