Research Topics
Species | F Eun Hyung LeeSummaryAffiliation: University of Rochester Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Circulating human antibody-secreting cells during vaccinations and respiratory viral infections are characterized by high specificity and lack of bystander effectF Eun Hyung Lee
Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
J Immunol 186:5514-21. 2011..Additionally, high specificity of circulating ASCs after antigenic challenge highlights the diagnostic value of interrogating ASCs as an ideal single-time-point diagnostic immune surrogate for serology during acute infection...
Circulating antibody-secreting cells during acute respiratory syncytial virus infection in adultsF Eun Hyung Lee
Division of Pulmonary and Critical Care Medicine, University of Rochester and Rochester General Hospital, Rochester, New York, USA
J Infect Dis 202:1659-66. 2010..The specificity and duration of circulating human antibody-secreting cells (ASCs) after vaccination have been well described, but characteristics of ASCs during acute respiratory infections have not been well studied...
The balance between influenza- and RSV-specific CD4 T cells secreting IL-10 or IFNgamma in young and healthy-elderly subjectsF Eun Hyung Lee
David H Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
Mech Ageing Dev 126:1223-9. 2005..A similar trend was seen for influenza. IFNgamma-secreting CD4 T cells contributed significantly more to anti-RSV than anti-influenza responses in both groups...- Fever and progressive respiratory failure in three elderly family membersF Eun-Hyung Lee
Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Rochester Medical Center, 601 Elmwood Ave, Box 692, Rochester, NY 14642, USA
Chest 128:1863-4, 1865-7. 2005 - Human infant respiratory syncytial virus (RSV)-specific type 1 and 2 cytokine responses ex vivo during primary RSV infectionF Eun Hyung Lee
David H Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
J Infect Dis 195:1779-88. 2007..Illness severity is likely multifactorial; however, unlike other viral infections, both type 1 and type 2 cytokine responses have been implicated in severe disease... - Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunizationJessica L Halliley
Division of Pulmonary and Critical Care Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
Vaccine 28:3582-7. 2010..The kinetics of this response and the relationship of circulating ASCs to protective antibody titers have not been completely explored... - A renewable source of donor cells for repetitive monitoring of T- and B-cell alloreactivityMartin S Zand
Nephrology Unit, Department of Medicine, University of Rochester Medical Center, Rochester, NY, USA
Am J Transplant 5:76-86. 2005..We conclude that sBc are a renewable source of donor-specific target/stimulator cells for use in repetitive and coordinate assays of B- and T-cell alloreactivity... - The effect of steroid use in hospitalized adults with respiratory syncytial virus-related illnessF Eun Hyung Lee
Division of Pulmonary and Critical Care Medicine, University of Rochester School of Medicine and Dentistry, NY, USA
Chest 140:1155-61. 2011..The effects of short-term high-dose steroid treatment on viral load and adaptive immunity to RSV have not been examined in adults... - T helper cytokine patterns: defined subsets, random expression, and external modulationTim R Mosmann
David H Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, NY 14642, USA
Immunol Res 45:173-84. 2009..Additional contributions to diversity may include new committed subsets, random expression of only part of the committed pattern, and modification of the expression patterns by cytokines and other mediators... - Regulation of antinucleoprotein IgG by systemic vaccination and its effect on influenza virus clearanceMark W Lamere
Department of Medicine, Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, New York 14642, USA
J Virol 85:5027-35. 2011..The high conservation of NP antigen and the known longevity of antibody responses suggest that the antiviral activity of anti-NP IgG may provide a critically needed component of a universal influenza vaccine... - Decreased influenza-specific B cell responses in rheumatoid arthritis patients treated with anti-tumor necrosis factorJames J Kobie
Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, 601 Elmwood Avenue, Box 695, Rochester, NY 14642, USA
Arthritis Res Ther 13:R209. 2011..Therefore, we asked if short-term effector (d5-d10) and memory (1 month or later) B cell responses to antigen were compromised in RA patients treated with anti-TNF therapy... - Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cellsShuya Y Kyu
Division of Pulmonary and Critical Care Medicine, NY 14642, USA
J Immunol Methods 340:42-7. 2009..43, 0.28, 0.28 respectively). These results demonstrate feasibility of testing whether antigen-specific ASC from frozen PBMC are an early biomarker of long-term antibody responses in multi-center vaccine trials... - Risk factors for respiratory failure associated with respiratory syncytial virus infection in adultsColey B Duncan
Department of Medicine, Rochester General Hospital, Rochester, New York 14621 3001, USA
J Infect Dis 200:1242-6. 2009..7 +/- 1.7 plaque-forming units (PFUs)/mL vs 2.4 +/- 1.1 PFUs/mL, P = .02), and high viral load was independently associated with respiratory failure... - Phenotypic and functional heterogeneity of human memory B cellsInaki Sanz
Division of Allergy, Immunology and Rheumatology, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Semin Immunol 20:67-82. 2008.... - Automated analysis of two- and three-color fluorescent Elispot (Fluorospot) assays for cytokine secretionJonathan A Rebhahn
David H Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY 14642, USA
Comput Methods Programs Biomed 92:54-65. 2008..The utility of Exploraspot is demonstrated by identification of single-, double- and triple-secreting T cells; tolerance of variable background fluorescence; and estimation of the numbers of genuine versus random multiple events... - B cells as therapeutic targets in SLEInaki Sanz
Division of Allergy, Immunology and Rheumatology, Department of Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
Nat Rev Rheumatol 6:326-37. 2010..Combined, the available information identifies several new avenues for the therapeutic targeting of B cells in SLE... - Antibodies against conserved antigens provide opportunities for reform in influenza vaccine designDenise A Kaminski
Division of Allergy, Immunology, and Rheumatology, Department of Medicine, University of Rochester Rochester, NY, USA
Front Immunol 2:76. 2011..These advances warrant further investigation of the inducibility and efficacy of such revolutionary antibody strategies in humans... - Protein vaccines induce uncommitted IL-2-secreting human and mouse CD4 T cells, whereas infections induce more IFN-gamma-secreting cellsAnagha A Divekar
David H. Smith Center for Vaccine Biology and Immunology, and Department of Microbiology and Immunology, University of Rochester, Rochester, NY 14642, USA
J Immunol 176:1465-73. 2006..This has implications for the design of more effective vaccines against pathogens requiring strong CD4 effector T cell responses... - Experimental infection of humans with A2 respiratory syncytial virusF Eun-Hyung Lee
Department of Medicine, University of Rochester School of Medicine and Dentistry, Box 689, 601 Elmwood Avenue, Rochester, NY 14642, USA
Antiviral Res 63:191-6. 2004..This model provides a safe and efficient mechanism for proof-of-concept studies of anti-viral agents and RSV vaccines, as well as for investigation of immune responses to infection... - Measuring the frequency of mouse and human cytotoxic T cells by the Lysispot assay: independent regulation of cytokine secretion and short-term killingJennifer E Snyder
David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, New York, USA
Nat Med 9:231-5. 2003..The simultaneous enumeration of cytokine-secreting and cytotoxic cells should be invaluable for ex vivo analysis of immune responses during infection and autoimmunity...
Research Grants
- Immune Mechanisms of Severe RSV Disease- T Cell and Antibody ResponsesFrances Lee; Fiscal Year: 2007..The goal of our studies is to provide insights in prevention and treatment of severe RSV disease in the elderly. ..