Ching Leang

Summary

Affiliation: University of Massachusetts
Country: USA

Publications

  1. pmc A genetic system for Clostridium ljungdahlii: a chassis for autotrophic production of biocommodities and a model homoacetogen
    Ching Leang
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    Appl Environ Microbiol 79:1102-9. 2013
  2. pmc Alignment of the c-type cytochrome OmcS along pili of Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Appl Environ Microbiol 76:4080-4. 2010
  3. pmc Genome-wide analysis of the RpoN regulon in Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    BMC Genomics 10:331. 2009
  4. pmc Adaptation to disruption of the electron transfer pathway for Fe(III) reduction in Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, 203 Morrill Science Center IVN, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    J Bacteriol 187:5918-26. 2005
  5. ncbi request reprint Regulation of two highly similar genes, omcB and omcC, in a 10 kb chromosomal duplication in Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, University of Massachusetts at Amherst, MA 01003, USA
    Microbiology 151:1761-7. 2005
  6. pmc Two putative c-type multiheme cytochromes required for the expression of OmcB, an outer membrane protein essential for optimal Fe(III) reduction in Geobacter sulfurreducens
    Byoung Chan Kim
    Department of Microbiology, 203 Morrill Science Center IVN, University of Massachusetts at Amherst, 639 North Pleasant Street, Amherst, MA 01003, USA
    J Bacteriol 188:3138-42. 2006
  7. pmc Aromatic amino acids required for pili conductivity and long-range extracellular electron transport in Geobacter sulfurreducens
    Madeline Vargas
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    MBio 4:e00105-13. 2013
  8. ncbi request reprint Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather than intracellular, respiration-linked Fe(III) citrate reduction
    Maddalena V Coppi
    Department of Microbiology, 203N Morrill Science Center IVN, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    Microbiology 153:3572-85. 2007
  9. pmc OmcF, a putative c-Type monoheme outer membrane cytochrome required for the expression of other outer membrane cytochromes in Geobacter sulfurreducens
    Byoung Chan Kim
    Department of Microbiology, 203 Morrill Science IVN, University of Massachusetts at Amherst, 639 North Pleasant St, Amherst, MA 01003, USA
    J Bacteriol 187:4505-13. 2005
  10. pmc OmcB, a c-type polyheme cytochrome, involved in Fe(III) reduction in Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Bacteriol 185:2096-103. 2003

Collaborators

Detail Information

Publications21

  1. pmc A genetic system for Clostridium ljungdahlii: a chassis for autotrophic production of biocommodities and a model homoacetogen
    Ching Leang
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    Appl Environ Microbiol 79:1102-9. 2013
    ..These results demonstrate the feasibility of genetic investigations of acetogen physiology and the potential for genetic manipulation of C. ljungdahlii to optimize autotrophic biocommodity production...
  2. pmc Alignment of the c-type cytochrome OmcS along pili of Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Appl Environ Microbiol 76:4080-4. 2010
    ..The apparent spacing between OmcS molecules suggests that OmcS facilitates electron transfer from pili to Fe(III) oxides rather than promoting electron conduction along the length of the pili...
  3. pmc Genome-wide analysis of the RpoN regulon in Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    BMC Genomics 10:331. 2009
    ....
  4. pmc Adaptation to disruption of the electron transfer pathway for Fe(III) reduction in Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, 203 Morrill Science Center IVN, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    J Bacteriol 187:5918-26. 2005
    ..sulfurreducens, which emphasizes the importance of studying electron transport to the environmentally relevant Fe(III) oxides...
  5. ncbi request reprint Regulation of two highly similar genes, omcB and omcC, in a 10 kb chromosomal duplication in Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, University of Massachusetts at Amherst, MA 01003, USA
    Microbiology 151:1761-7. 2005
    ..These results indicate that omcB and omcC operons represent a rare instance in which duplicated operons, located in tandem on the chromosome, have different transcriptional regulation...
  6. pmc Two putative c-type multiheme cytochromes required for the expression of OmcB, an outer membrane protein essential for optimal Fe(III) reduction in Geobacter sulfurreducens
    Byoung Chan Kim
    Department of Microbiology, 203 Morrill Science Center IVN, University of Massachusetts at Amherst, 639 North Pleasant Street, Amherst, MA 01003, USA
    J Bacteriol 188:3138-42. 2006
    ..Expression of either gene restored Fe(III) reduction and OmcB expression, suggesting functional similarity...
  7. pmc Aromatic amino acids required for pili conductivity and long-range extracellular electron transport in Geobacter sulfurreducens
    Madeline Vargas
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    MBio 4:e00105-13. 2013
    ..These results demonstrate that the pili of G. sulfurreducens must be conductive in order for the cells to be effective in extracellular long-range electron transport...
  8. ncbi request reprint Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather than intracellular, respiration-linked Fe(III) citrate reduction
    Maddalena V Coppi
    Department of Microbiology, 203N Morrill Science Center IVN, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    Microbiology 153:3572-85. 2007
    ..These results indicate that cytoplasmic, respiration-linked reduction of Fe(III) by SfrAB in vivo is unlikely and suggest that deleting SfrAB may interfere with growth via acetate oxidation by interfering with NADP regeneration...
  9. pmc OmcF, a putative c-Type monoheme outer membrane cytochrome required for the expression of other outer membrane cytochromes in Geobacter sulfurreducens
    Byoung Chan Kim
    Department of Microbiology, 203 Morrill Science IVN, University of Massachusetts at Amherst, 639 North Pleasant St, Amherst, MA 01003, USA
    J Bacteriol 187:4505-13. 2005
    ..Thus, elimination of OmcF may impair Fe(III) reduction by influencing expression of OmcB, which has previously been demonstrated to play a critical role in Fe(III) reduction...
  10. pmc OmcB, a c-type polyheme cytochrome, involved in Fe(III) reduction in Geobacter sulfurreducens
    Ching Leang
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Bacteriol 185:2096-103. 2003
    ..These results indicate that OmcB, but not OmcC, has a major role in electron transport to Fe(III) and suggest that electron transport to the outer membrane is an important feature in Fe(III) reduction in this organism...
  11. doi request reprint Direct exchange of electrons within aggregates of an evolved syntrophic coculture of anaerobic bacteria
    Zarath M Summers
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    Science 330:1413-5. 2010
    ..Aggregate formation was also much faster in mutants that were deficient in interspecies hydrogen transfer, further suggesting direct interspecies electron transfer...
  12. pmc Importance of c-Type cytochromes for U(VI) reduction by Geobacter sulfurreducens
    Evgenya S Shelobolina
    Department of Microbiology, University of Massachusetts, Amherst, MA, USA
    BMC Microbiol 7:16. 2007
    ..In order to study the mechanism of U(VI) reduction, the effect of deleting c-type cytochrome genes on the capacity of Geobacter sulfurreducens to reduce U(VI) with acetate serving as the electron donor was investigated...
  13. doi request reprint Tunable metallic-like conductivity in microbial nanowire networks
    Nikhil S Malvankar
    Department of Physics, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Nat Nanotechnol 6:573-9. 2011
    ..The conductivity of the nanofilaments has a temperature dependence similar to that of a disordered metal, and the conductivity could be increased by processing...
  14. doi request reprint Specific localization of the c-type cytochrome OmcZ at the anode surface in current-producing biofilms of Geobacter sulfurreducens
    Kengo Inoue
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA Interdisciplinary Research Organization, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889 1692, Japan
    Environ Microbiol Rep 3:211-7. 2011
    ..sulfurreducens biofilms to the anode surface...
  15. pmc U(VI) reduction by diverse outer surface c-type cytochromes of Geobacter sulfurreducens
    Roberto Orellana
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    Appl Environ Microbiol 79:6369-74. 2013
    ..These findings are consistent with those of previous studies, which have suggested that G. sulfurreducens requires outer-surface c-type cytochromes but not pili for the reduction of soluble extracellular electron acceptors. ..
  16. pmc The Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD+ oxidoreductase essential for autotrophic growth
    Pier Luc Tremblay
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    MBio 4:e00406-12. 2012
    ..ljungdahlii is responsible for translocation of protons across the membrane to elicit energy conservation during acetogenesis and is a multifunctional device also implicated in nitrogen fixation...
  17. pmc Identification of multicomponent histidine-aspartate phosphorelay system controlling flagellar and motility gene expression in Geobacter species
    Toshiyuki Ueki
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 287:10958-66. 2012
    ..Therefore, the multicomponent His-Asp phosphorelay system appears to control flagellar gene expression in Geobacter species...
  18. pmc Direct correlation between rates of anaerobic respiration and levels of mRNA for key respiratory genes in Geobacter sulfurreducens
    Kuk Jeong Chin
    Department of Microbiology, University of Massachusetts, Morrill Science Center IV North, 639 North Pleasant St, Amherst, MA 01003, USA
    Appl Environ Microbiol 70:5183-9. 2004
    ....
  19. ncbi request reprint Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii
    Areen Banerjee
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    Appl Environ Microbiol 80:2410-6. 2014
    ..Furthermore, this tool should aid in optimizing microbial electrosynthesis and for basic studies on the physiology of acetogenesis...
  20. doi request reprint A genetic system for Geobacter metallireducens: role of the flagellin and pilin in the reduction of Fe(III) oxide
    Pier Luc Tremblay
    Department of Microbiology, University of Massachusetts, Amherst, MA, USA
    Environ Microbiol Rep 4:82-8. 2012
    ..These results demonstrate the importance of flagella and pili in the reduction of insoluble Fe(III) by G. metallireducens and provide methods for additional genetic-based approaches for the study of G. metallireducens...
  21. pmc Biochemical and genetic characterization of PpcA, a periplasmic c-type cytochrome in Geobacter sulfurreducens
    Jon R Lloyd
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    Biochem J 369:153-61. 2003
    ....