EDUARDO RODOLFO LAZAROWSKI

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc The UDP-sugar-sensing P2Y(14) receptor promotes Rho-mediated signaling and chemotaxis in human neutrophils
    Juliana I Sesma
    Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol Cell Physiol 303:C490-8. 2012
  2. pmc Vesicular and conductive mechanisms of nucleotide release
    Eduardo R Lazarowski
    School of Medicine, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Purinergic Signal 8:359-73. 2012
  3. pmc Calcium-dependent release of adenosine and uridine nucleotides from A549 cells
    Sabina Tatur
    Research Centre, Centre Hospitalier de l Universite de Montreal CHUM, Hotel Dieu, 3850 Rue Saint Urbain, Montreal, Quebec, H2W 1T7, Canada
    Purinergic Signal 4:139-46. 2008
  4. doi request reprint Nucleotide release by airway epithelia
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, 7011 Thurston Bowles Building, Chapel Hill, NC, 27599, USA
    Subcell Biochem 55:1-15. 2011
  5. pmc Quantification of extracellular UDP-galactose
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Medicine, 7017 Thurston Bowles Building, CB 7248, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Anal Biochem 396:23-9. 2010
  6. pmc Purinergic receptors in airway epithelia
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Opin Pharmacol 9:262-7. 2009
  7. ncbi request reprint Constitutive release of ATP and evidence for major contribution of ecto-nucleotide pyrophosphatase and nucleoside diphosphokinase to extracellular nucleotide concentrations
    E R Lazarowski
    Departments of Medicine and Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 275:31061-8. 2000
  8. pmc Nucleotide release provides a mechanism for airway surface liquid homeostasis
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina School of Medicine, 7017 Thurston Bowles Building, Chapel Hill, NC 72599, USA
    J Biol Chem 279:36855-64. 2004
  9. ncbi request reprint Cloning and functional characterization of two murine uridine nucleotide receptors reveal a potential target for correcting ion transport deficiency in cystic fibrosis gallbladder
    E R Lazarowski
    University of North Carolina School of Medicine, Department of Medicine, Cystic Fibrosis Pulmonary Research and Treatment Center, CB 7248, 7017 Thurston Bowles Building, Chapel Hill, NC 27599 7248, USA
    J Pharmacol Exp Ther 297:43-9. 2001
  10. pmc Normal and cystic fibrosis airway surface liquid homeostasis. The effects of phasic shear stress and viral infections
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 280:35751-9. 2005

Research Grants

  1. Mechanisms of Nucleotide Release by Airway Epithelia
    Eduardo Lazarowski; Fiscal Year: 2006

Collaborators

Detail Information

Publications29

  1. pmc The UDP-sugar-sensing P2Y(14) receptor promotes Rho-mediated signaling and chemotaxis in human neutrophils
    Juliana I Sesma
    Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol Cell Physiol 303:C490-8. 2012
    ..These results support the notion that UDP-glucose is a stable and potent proinflammatory mediator that promotes P2Y(14)-R-mediated neutrophil motility via Rho/Rho kinase activation...
  2. pmc Vesicular and conductive mechanisms of nucleotide release
    Eduardo R Lazarowski
    School of Medicine, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Purinergic Signal 8:359-73. 2012
    ..In addition, plasma membrane connexin hemichannels, pannexin channels, and less-well molecularly defined ATP conducting anion channels have been shown to contribute to the release of ATP (and UTP) under a variety of conditions...
  3. pmc Calcium-dependent release of adenosine and uridine nucleotides from A549 cells
    Sabina Tatur
    Research Centre, Centre Hospitalier de l Universite de Montreal CHUM, Hotel Dieu, 3850 Rue Saint Urbain, Montreal, Quebec, H2W 1T7, Canada
    Purinergic Signal 4:139-46. 2008
    ..In summary, our results strongly suggest that calcium-dependent exocytosis is responsible, at least in most part, for adenosine and uridine nucleotide release from A549 cells...
  4. doi request reprint Nucleotide release by airway epithelia
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, 7011 Thurston Bowles Building, Chapel Hill, NC, 27599, USA
    Subcell Biochem 55:1-15. 2011
    ..Together, these pathways provide fine tuning of epithelial responses regulated by purinergic signaling events...
  5. pmc Quantification of extracellular UDP-galactose
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Medicine, 7017 Thurston Bowles Building, CB 7248, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Anal Biochem 396:23-9. 2010
    ..The occurrence of regulated release of UDP-Gal suggests that, in addition to its role in glycosylation reactions, UDP-Gal is an important extracellular signaling molecule...
  6. pmc Purinergic receptors in airway epithelia
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Opin Pharmacol 9:262-7. 2009
    ..Agonist occupation of the P2Y2 receptor promotes inhibition of Na+ absorption as well as CFTR-dependent and CFTR-independent Cl- secretion, ciliary beating, and mucin secretion...
  7. ncbi request reprint Constitutive release of ATP and evidence for major contribution of ecto-nucleotide pyrophosphatase and nucleoside diphosphokinase to extracellular nucleotide concentrations
    E R Lazarowski
    Departments of Medicine and Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 275:31061-8. 2000
    ..In addition to the existence of an ecto-ATPase activity, our results suggest a major scavenger role of ecto-ATP pyrophosphatase and a transphosphorylating activity of nucleoside diphosphokinase...
  8. pmc Nucleotide release provides a mechanism for airway surface liquid homeostasis
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina School of Medicine, 7017 Thurston Bowles Building, Chapel Hill, NC 72599, USA
    J Biol Chem 279:36855-64. 2004
    ..Our data reveal a complex pattern of nucleotides/nucleosides in ASL under resting conditions and suggest that adenosine may play a key role in regulating ASL volume homeostasis...
  9. ncbi request reprint Cloning and functional characterization of two murine uridine nucleotide receptors reveal a potential target for correcting ion transport deficiency in cystic fibrosis gallbladder
    E R Lazarowski
    University of North Carolina School of Medicine, Department of Medicine, Cystic Fibrosis Pulmonary Research and Treatment Center, CB 7248, 7017 Thurston Bowles Building, Chapel Hill, NC 27599 7248, USA
    J Pharmacol Exp Ther 297:43-9. 2001
    ....
  10. pmc Normal and cystic fibrosis airway surface liquid homeostasis. The effects of phasic shear stress and viral infections
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 280:35751-9. 2005
    ....
  11. ncbi request reprint Mechanical ventilation alters airway nucleotides and purinoceptors in lung and extrapulmonary organs
    Christelle D Douillet
    Department of Surgery, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7228, USA
    Am J Respir Cell Mol Biol 32:52-8. 2005
    ..MV alters the BAL adenyl-nucleotide profile and purinoceptor patterns in lung, liver, and kidney. PEEP normalizes the BAL nucleotide profile and receptor patterns in lung and extrapulmonary tissues...
  12. pmc Thrombin promotes release of ATP from lung epithelial cells through coordinated activation of rho- and Ca2+-dependent signaling pathways
    Lucia Seminario-Vidal
    Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 284:20638-48. 2009
    ....
  13. pmc Endoplasmic reticulum/golgi nucleotide sugar transporters contribute to the cellular release of UDP-sugar signaling molecules
    Juliana I Sesma
    Department of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 284:12572-83. 2009
    ..Our results illustrate that ER/Golgi lumen constitutes a significant source of extracellular UDP-sugars and therefore plays a critical role in nucleotide sugar-promoted cell signaling...
  14. pmc Neutrophilic inflammation is associated with altered airway hydration in stable asthmatics
    Ceila E Loughlin
    Department of Pediatrics, Division of Pediatric Pulmonology, The School of Medicine, University of North Carolina, 130 Mason Farm Road, 5th floor Bioinformatics Bldg, CB 7217, Chapel Hill, NC 27599 7217, USA
    Respir Med 104:29-33. 2010
    ..Using sputum percent solids, as an indicator of airway hydration, we sought relationships between airway hydration and other known markers of neutrophilic (TH1) and allergic (TH2) inflammation in stable asthma...
  15. pmc Receptor-promoted exocytosis of airway epithelial mucin granules containing a spectrum of adenine nucleotides
    Silvia M Kreda
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, 4029A Thurston Bowles Building, Chapel Hill, NC 27599 7248, USA
    J Physiol 588:2255-67. 2010
    ....
  16. pmc Gi-dependent cell signaling responses of the human P2Y14 receptor in model cell systems
    Ingrid P Fricks
    Department of Pharmacology, University of North Carolina, School of Medicine, Chapel Hill, NC 27599, USA
    J Pharmacol Exp Ther 330:162-8. 2009
    ..This work also identifies two stable P2Y(14)-R-expressing cell lines and differentiated HL-60 cells as model systems for the study of P2Y(14)-R-dependent signal transduction...
  17. pmc Similarities between UDP-glucose and adenine nucleotide release in yeast: involvement of the secretory pathway
    Charles R Esther
    Division of Pediatric Pulmonology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 47:9269-78. 2008
    ..These studies demonstrate the involvement of the secretory pathway in nucleotide and nucleotide sugar efflux in yeast and offer a powerful model system for further investigation...
  18. pmc Voltage-dependent anion channel-1 (VDAC-1) contributes to ATP release and cell volume regulation in murine cells
    Seiko F Okada
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Gen Physiol 124:513-26. 2004
    ..However, the observation that VDAC-1 knockout cells released a significant amount of ATP suggests that other molecules also play a role in this function...
  19. pmc Physiological regulation of ATP release at the apical surface of human airway epithelia
    Seiko F Okada
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC, USA
    J Biol Chem 281:22992-3002. 2006
    ....
  20. pmc Coordinated release of nucleotides and mucin from human airway epithelial Calu-3 cells
    Silvia M Kreda
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 7017 Thurston Bowles Building, Chapel Hill, NC 27599 7248, USA
    J Physiol 584:245-59. 2007
    ..Our data suggest that nucleotide release is a mechanism by which mucin-secreting goblet cells produce paracrine signals for mucin hydration within the ASL...
  21. pmc Mathematical model of nucleotide regulation on airway epithelia. Implications for airway homeostasis
    Peiying Zuo
    Department of Mathematics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 283:26805-19. 2008
    ..These new insights into the biochemical processes supporting ASL nucleotide regulation illustrate the potential of this mathematical model for fundamental and clinical research...
  22. ncbi request reprint Adenosine deaminase 1 and concentrative nucleoside transporters 2 and 3 regulate adenosine on the apical surface of human airway epithelia: implications for inflammatory lung diseases
    Andrew J Hirsh
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 46:10373-83. 2007
    ..This study shows that adenosine elimination on human airway epithelia is mediated by ADA1, CNT2, and CNT3, which constitute important regulators of adenosine-mediated inflammation...
  23. ncbi request reprint [Purinergic signals]
    Eduardo R Lazarowski
    School of Medicine, Department of Medicine, The Cystic Fibrosis Treatment and Research Center, University of North Carolina, USA
    Medicina (B Aires) 69:267-76. 2009
    ..Particular focus is given to platelet aggregation and thrombus formation, the immune response and the hydration of the mucosal linings of the respiratory tract...
  24. ncbi request reprint Mechanisms of release of nucleotides and integration of their action as P2X- and P2Y-receptor activating molecules
    Eduardo R Lazarowski
    Department of Pharmacology, University of North Carolina School of Medicine, CB 7365, Chapel Hill, NC 27599, USA
    Mol Pharmacol 64:785-95. 2003
  25. doi request reprint Assessment of extracellular ATP concentrations
    Lucia Seminario-Vidal
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Methods Mol Biol 574:25-36. 2009
    ..Although our focus is on studies of ATP release from epithelial cells, protocols described here are applicable to practically all cell types...
  26. pmc Exposure to hydrogen peroxide induces oxidation and activation of AMP-activated protein kinase
    Jaroslaw W Zmijewski
    Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0012, USA
    J Biol Chem 285:33154-64. 2010
    ..The present findings also imply that AMPK activation, in addition to being a response to alterations in intracellular metabolic pathways, is directly influenced by cellular redox status...
  27. pmc Quantification of Gi-mediated inhibition of adenylyl cyclase activity reveals that UDP is a potent agonist of the human P2Y14 receptor
    Rhonda L Carter
    Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC, USA
    Mol Pharmacol 76:1341-8. 2009
    ..We conclude that UDP is an important cognate agonist of the human P2Y14 receptor...
  28. ncbi request reprint Release of cellular UDP-glucose as a potential extracellular signaling molecule
    Eduardo R Lazarowski
    Department of Medicine, Cystic Fibrosis Center, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    Mol Pharmacol 63:1190-7. 2003
    ..Because cellular UDP-glucose is concentrated in the lumen of the endoplasmic reticulum, we speculate that UDP-glucose release may occur as a result of vesicle transport during trafficking of glycoproteins to the plasma membrane...
  29. pmc UDP is a competitive antagonist at the human P2Y14 receptor
    Ingrid P Fricks
    Department of Pharmacology, University of North Carolina, CB 7365 Mary Ellen Jones Bldg, Chapel Hill, NC 27599 7365, USA
    J Pharmacol Exp Ther 325:588-94. 2008
    ..35 muM) at the rat P2Y(14)-R. These results identify the first competitive antagonist of the P2Y(14)-R and demonstrate pharmacological differences between receptor orthologs...

Research Grants1

  1. Mechanisms of Nucleotide Release by Airway Epithelia
    Eduardo Lazarowski; Fiscal Year: 2006
    ..Delineation of nucleotide-releasing mechanisms will provide new possibilities to improve MCC function in chronic lung diseases characterized by mucin-obstructed and inflamed airways. ..