Nathan D Lawson

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. pmc Initiation of zebrafish haematopoiesis by the TATA-box-binding protein-related factor Trf3
    Daniel O Hart
    Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 450:1082-5. 2007
  2. pmc Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases
    Ankit Gupta
    Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Nucleic Acids Res 39:381-92. 2011
  3. pmc MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis
    Stefania Nicoli
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 464:1196-200. 2010
  4. doi request reprint Forward and reverse genetic approaches for the analysis of vertebrate development in the zebrafish
    Nathan D Lawson
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Cell 21:48-64. 2011
  5. pmc phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development
    Nathan D Lawson
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:1346-51. 2003
  6. pmc Combinatorial function of ETS transcription factors in the developing vasculature
    Van N Pham
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development NIH, Building 6B, Room 309, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 303:772-83. 2007
  7. pmc Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish
    Cong Zhu
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 138:4555-64. 2011
  8. doi request reprint Post-transcriptional mechanisms contribute to Etv2 repression during vascular development
    John C Moore
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA Electronic address
    Dev Biol 384:128-40. 2013
  9. ncbi request reprint sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 3:127-36. 2002
  10. pmc A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development
    Jacques A Villefranc
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 140:1497-506. 2013

Research Grants

Collaborators

Detail Information

Publications36

  1. pmc Initiation of zebrafish haematopoiesis by the TATA-box-binding protein-related factor Trf3
    Daniel O Hart
    Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 450:1082-5. 2007
    ..Thus, in zebrafish, commitment of mesoderm to the haematopoietic lineage occurs through a transcription factor pathway initiated by a TBP-related factor...
  2. pmc Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases
    Ankit Gupta
    Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Nucleic Acids Res 39:381-92. 2011
    ....
  3. pmc MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis
    Stefania Nicoli
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 464:1196-200. 2010
    ..Taken together, our work describes a novel genetic mechanism in which a microRNA facilitates integration of a physiological stimulus with growth factor signalling in endothelial cells to guide angiogenesis...
  4. doi request reprint Forward and reverse genetic approaches for the analysis of vertebrate development in the zebrafish
    Nathan D Lawson
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Cell 21:48-64. 2011
    ....
  5. pmc phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development
    Nathan D Lawson
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:1346-51. 2003
    ..Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system...
  6. pmc Combinatorial function of ETS transcription factors in the developing vasculature
    Van N Pham
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development NIH, Building 6B, Room 309, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 303:772-83. 2007
    ..Our results demonstrate that combinatorial ETS factor function is essential for early endothelial specification and differentiation...
  7. pmc Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish
    Cong Zhu
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 138:4555-64. 2011
    ..This work provides a resource to allow targeted germline gene inactivation in zebrafish and highlights the benefit of a definitive reverse genetic strategy to reveal gene function...
  8. doi request reprint Post-transcriptional mechanisms contribute to Etv2 repression during vascular development
    John C Moore
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA Electronic address
    Dev Biol 384:128-40. 2013
    ..Taken together, our results suggest that etv2 acts during early development to specify endothelial lineages and is then down-regulated, in part through post-transcriptional repression by microRNAs, to allow normal vascular development...
  9. ncbi request reprint sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 3:127-36. 2002
    ..These studies reveal a complex signaling cascade responsible for establishing arterial cell fate and suggest differential effects of Vegf on developing endothelial cells...
  10. pmc A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development
    Jacques A Villefranc
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 140:1497-506. 2013
    ....
  11. pmc Chemokine signaling guides regional patterning of the first embryonic artery
    Arndt F Siekmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01602, USA
    Genes Dev 23:2272-7. 2009
    ..These studies reveal unexpected endothelial diversity within the aorta that is necessary to facilitate its regional patterning by local cues...
  12. pmc miR-221 is required for endothelial tip cell behaviors during vascular development
    Stefania Nicoli
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Cell 22:418-29. 2012
    ..These results identify miR-221 as an important regulatory node through which tip cell migration and proliferation are controlled during angiogenesis...
  13. ncbi request reprint Distinct Notch signaling outputs pattern the developing arterial system
    Aurelie Quillien
    Program in Gene Function and Expression, UMass Medical School, Worcester, MA 01605 USA
    Development 141:1544-52. 2014
    ..Together, these findings demonstrate that Notch acts in distinct contexts to initiate and maintain artery identity during embryogenesis. ..
  14. ncbi request reprint Construction and application of site-specific artificial nucleases for targeted gene editing
    Fatma O Kok
    Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, MA, USA
    Methods Mol Biol 1101:267-303. 2014
    ..In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome. ..
  15. pmc Identification of cis regulatory features in the embryonic zebrafish genome through large-scale profiling of H3K4me1 and H3K4me3 binding sites
    Aaron W Aday
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01602, USA
    Dev Biol 357:450-62. 2011
    ....
  16. ncbi request reprint Notch signalling limits angiogenic cell behaviour in developing zebrafish arteries
    Arndt F Siekmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 445:781-4. 2007
    ..Together, these studies indicate that proper specification of cell identity, position and behaviour in a developing blood-vessel sprout is required for normal angiogenesis, and implicate the Notch signalling pathway in this process...
  17. pmc Centrin depletion causes cyst formation and other ciliopathy-related phenotypes in zebrafish
    Benedicte Delaval
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
    Cell Cycle 10:3964-72. 2011
    ..Moreover, given the presence of both cilia and mitotic defects in the affected organs, it suggests that cilia disorders may arise from a combination of these defects...
  18. pmc Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
    Xiangdong Meng
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 6th Floor, 364 Plantation St, Worcester, Massachusetts 01605, USA
    Nat Biotechnol 26:695-701. 2008
    ..The use of engineered ZFNs to introduce heritable mutations into a genome obviates the need for embryonic stem cell lines and should be applicable to most animal species for which early-stage embryos are easily accessible...
  19. ncbi request reprint Angiogenic network formation in the developing vertebrate trunk
    Sumio Isogai
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Development 130:5281-90. 2003
    ..We discuss a model to explain our results combining genetic programming of overall vascular architecture with hemodynamic determination of circulatory flow patterns...
  20. pmc Targeted chromosomal deletions and inversions in zebrafish
    Ankit Gupta
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genome Res 23:1008-17. 2013
    ..The ability to efficiently delete genomic segments in a vertebrate developmental system will facilitate the study of functional noncoding elements on an organismic level...
  21. pmc The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney
    Sung Kook Hong
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:18473-8. 2005
    ..These results suggest that critical targets of TRAP230 function may include proteins important for cell mobility, cell sorting, and tissue assembly...
  22. ncbi request reprint Disruption of acvrl1 increases endothelial cell number in zebrafish cranial vessels
    Beth L Roman
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Development 129:3009-19. 2002
    ..Movies available on-line..
  23. doi request reprint Modulation of VEGF signalling output by the Notch pathway
    Arndt F Siekmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Bioessays 30:303-13. 2008
    ..In this review, we provide an overview of these biochemically distinct pathways and discuss how they may interact during endothelial cell differentiation and angiogenesis...
  24. ncbi request reprint Gateway compatible vectors for analysis of gene function in the zebrafish
    Jacques A Villefranc
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Dev Dyn 236:3077-87. 2007
    ..Taken together, this work provides an important platform for the rapid functional analyses of open reading frames in zebrafish embryos...
  25. ncbi request reprint In vivo imaging of embryonic vascular development using transgenic zebrafish
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 248:307-18. 2002
    ....
  26. pmc Global analysis of hematopoietic and vascular endothelial gene expression by tissue specific microarray profiling in zebrafish
    Laurence Covassin
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, Room 617, 364 Plantation Street, Worcester, MA 01605, USA
    Dev Biol 299:551-62. 2006
    ..Our results demonstrate that this is a robust approach for identification of cell type specific genes as well as for global analysis of cell type specific gene expression in zebrafish embryos...
  27. ncbi request reprint Arteries and veins: making a difference with zebrafish
    Nathan D Lawson
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Building 6B, Room 309, 6 Center Drive, Bethesda, Maryland 20892, USA
    Nat Rev Genet 3:674-82. 2002
    ....
  28. pmc A nonsense mutation in zebrafish gata1 causes the bloodless phenotype in vlad tepes
    Susan E Lyons
    National Human Genome Research Institute, National Institutes of Health, Building 49, Room 3A18, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:5454-9. 2002
    ....
  29. pmc ChIPpeakAnno: a Bioconductor package to annotate ChIP-seq and ChIP-chip data
    Lihua J Zhu
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    BMC Bioinformatics 11:237. 2010
    ..However, summarizing these tracks can be a daunting task, particularly if there are a large number of binding sites or the binding sites are distributed widely across the genome...
  30. pmc The cilia protein IFT88 is required for spindle orientation in mitosis
    Benedicte Delaval
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Suite 210, Worcester, Massachusetts 01605, USA
    Nat Cell Biol 13:461-8. 2011
    ..This work identifies a mitotic mechanism for a cilia protein in the orientation of cell division and has important implications for the etiology of ciliopathies...
  31. pmc Notch signalling and the regulation of angiogenesis
    Arndt F Siekmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Cell Adh Migr 1:104-6. 2007
    ..In this overview, we discuss the emerging concepts elucidated by these studies and propose a model in which Notch acts reiteratively throughout the angiogenic process, likely by acting as a switch to determine a cell's response to Vegf...
  32. pmc Accurate identification of polyadenylation sites from 3' end deep sequencing using a naive Bayes classifier
    Sarah Sheppard
    Program in Gene Function and Expression and Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
    Bioinformatics 29:2564-71. 2013
    ..Therefore, there is a need to develop improved algorithms to better identify mis-priming events in oligo-dT primed sequences...
  33. ncbi request reprint An essential role for Fgfs in endodermal pouch formation influences later craniofacial skeletal patterning
    Justin Gage Crump
    Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403 1254, USA
    Development 131:5703-16. 2004
    ..Moreover, we argue that the Fgf-dependent morphogenesis of the pharyngeal endoderm into pouches is critical for the later patterning of pharyngeal cartilages...
  34. pmc reg6 is required for branching morphogenesis during blood vessel regeneration in zebrafish caudal fins
    Cheng chen Huang
    Department of Genetics, Washington University Medical School, St Louis, MO 63110, USA
    Dev Biol 264:263-74. 2003
    ..Our results suggest that the reg6 mutation causes defects in branch formation and/or angiogenic sprouting...
  35. ncbi request reprint Arteries define the position of the thyroid gland during its developmental relocalisation
    Burkhard Alt
    Institute for Developmental Biology, University of Cologne, Gyrhofstrasse 17, Koln, Germany
    Development 133:3797-804. 2006
    ..Moreover, the involvement of vessels in thyroid relocalisation sheds new light on the interpretation of congenital thyroid defects in humans...
  36. pmc pak2a mutations cause cerebral hemorrhage in redhead zebrafish
    David A Buchner
    Howard Hughes Medical Institute and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 104:13996-4001. 2007
    ..These data provide in vivo evidence for a critical function of Pak2 in vascular integrity and demonstrate a severe disease phenotype resulting from loss of Pak2 function...

Research Grants18

  1. Transcriptional Control of Endothelial Differentiation
    Nathan D Lawson; Fiscal Year: 2010
    ..However, little is known about how these differences arise. In this proposal, we will utilize the zebra fish as a model system to identify gene programs that govern blood vessel identity in the developing embryo. ..
  2. Transgenic screen for artery development mutants
    Nathan Lawson; Fiscal Year: 2004
    ..Together, these studies will allow identification of genes that are required for proper artery and vein development and will represent putative targets for therapeutic manipulation. ..
  3. Reverse Genetic Screening for Vascular Mutants Using Zinc Finger Nucleases
    Nathan D Lawson; Fiscal Year: 2010
    ..In this proposal, we will apply new technology to generate zebrafish knockouts in more than 30 genes to investigate their function during vascular development. ..
  4. Using zinc finger nucleases to manipulate the zebrafish genome
    Scot A Wolfe; Fiscal Year: 2010
    ..We will apply this technology in zebrafish to better understand the processes involved in heart regeneration and blood vessel formation. ..
  5. Transgenic screen for artery development mutants
    Nathan Lawson; Fiscal Year: 2005
    ..Together, these studies will allow identification of genes that are required for proper artery and vein development and will represent putative targets for therapeutic manipulation. ..
  6. Role of Plcg1 in Vegf signaling
    Nathan Lawson; Fiscal Year: 2005
    ..Since these signaling mechanisms are evolutionary conserved, the molecules we identify in this proposal represent possible targets for clinical manipulation of pathological blood vessel formation. ..
  7. Using zinc finger nucleases to manipulate the zebrafish genome
    Nathan Lawson; Fiscal Year: 2009
    ..We will apply this technology in zebrafish to better understand the processes involved in heart regeneration and blood vessel formation. ..
  8. Role of Notch in artery development
    Nathan Lawson; Fiscal Year: 2009
    ..The studies in this proposal will address how blood vessel identities are determined during development. ..
  9. Role of Plcg1 in Vegf signaling
    Nathan Lawson; Fiscal Year: 2009
    ..Since these signaling mechanisms are evolutionary conserved, the molecules we identify in this proposal represent possible targets for clinical manipulation of pathological blood vessel formation. ..
  10. Identifying Vegf target genes during artery development
    Nathan Lawson; Fiscal Year: 2005
    ....
  11. Transgenic screen for artery development mutants
    Nathan Lawson; Fiscal Year: 2007
    ..Together, these studies will allow identification of genes that are required for proper artery and vein development and will represent putative targets for therapeutic manipulation. ..
  12. Transgenic screen for artery development mutants
    Nathan Lawson; Fiscal Year: 2006
    ..Together, these studies will allow identification of genes that are required for proper artery and vein development and will represent putative targets for therapeutic manipulation. ..
  13. Role of Plcg1 in Vegf signaling
    Nathan Lawson; Fiscal Year: 2007
    ..Since these signaling mechanisms are evolutionary conserved, the molecules we identify in this proposal represent possible targets for clinical manipulation of pathological blood vessel formation. ..
  14. Role of Notch in artery development
    Nathan D Lawson; Fiscal Year: 2010
    ..The studies in this proposal will address how blood vessel identities are determined during development. ..
  15. Role of Plcg1 in Vegf signaling
    Nathan Lawson; Fiscal Year: 2006
    ..Since these signaling mechanisms are evolutionary conserved, the molecules we identify in this proposal represent possible targets for clinical manipulation of pathological blood vessel formation. ..