Research Topics
Genomes and Genes | Nathan D LawsonSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
Research Grants
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Publications
Initiation of zebrafish haematopoiesis by the TATA-box-binding protein-related factor Trf3Daniel O Hart
Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
Nature 450:1082-5. 2007..Thus, in zebrafish, commitment of mesoderm to the haematopoietic lineage occurs through a transcription factor pathway initiated by a TBP-related factor...
Forward and reverse genetic approaches for the analysis of vertebrate development in the zebrafishNathan D Lawson
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
Dev Cell 21:48-64. 2011....- phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial developmentNathan D Lawson
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Genes Dev 17:1346-51. 2003..Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system... - Combinatorial function of ETS transcription factors in the developing vasculatureVan N Pham
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development NIH, Building 6B, Room 309, 6 Center Drive, Bethesda, MD 20892, USA
Dev Biol 303:772-83. 2007..Our results demonstrate that combinatorial ETS factor function is essential for early endothelial specification and differentiation... - Evaluation and application of modularly assembled zinc-finger nucleases in zebrafishCong Zhu
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
Development 138:4555-64. 2011..This work provides a resource to allow targeted germline gene inactivation in zebrafish and highlights the benefit of a definitive reverse genetic strategy to reveal gene function... - MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesisStefania Nicoli
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nature 464:1196-200. 2010..Taken together, our work describes a novel genetic mechanism in which a microRNA facilitates integration of a physiological stimulus with growth factor signalling in endothelial cells to guide angiogenesis... 
sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiationNathan D Lawson
Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
Dev Cell 3:127-36. 2002..These studies reveal a complex signaling cascade responsible for establishing arterial cell fate and suggest differential effects of Vegf on developing endothelial cells...- miR-221 is required for endothelial tip cell behaviors during vascular developmentStefania Nicoli
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
Dev Cell 22:418-29. 2012..These results identify miR-221 as an important regulatory node through which tip cell migration and proliferation are controlled during angiogenesis... - Identification of cis regulatory features in the embryonic zebrafish genome through large-scale profiling of H3K4me1 and H3K4me3 binding sitesAaron W Aday
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01602, USA
Dev Biol 357:450-62. 2011.... - Centrin depletion causes cyst formation and other ciliopathy-related phenotypes in zebrafishBenedicte Delaval
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Cell Cycle 10:3964-72. 2011..Moreover, given the presence of both cilia and mitotic defects in the affected organs, it suggests that cilia disorders may arise from a combination of these defects... - Chemokine signaling guides regional patterning of the first embryonic arteryArndt F Siekmann
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01602, USA
Genes Dev 23:2272-7. 2009..These studies reveal unexpected endothelial diversity within the aorta that is necessary to facilitate its regional patterning by local cues... - Notch signalling limits angiogenic cell behaviour in developing zebrafish arteriesArndt F Siekmann
Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, Massachusetts 01605, USA
Nature 445:781-4. 2007..Together, these studies indicate that proper specification of cell identity, position and behaviour in a developing blood-vessel sprout is required for normal angiogenesis, and implicate the Notch signalling pathway in this process... - Angiogenic network formation in the developing vertebrate trunkSumio Isogai
Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
Development 130:5281-90. 2003..We discuss a model to explain our results combining genetic programming of overall vascular architecture with hemodynamic determination of circulatory flow patterns... - The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidneySung Kook Hong
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:18473-8. 2005..These results suggest that critical targets of TRAP230 function may include proteins important for cell mobility, cell sorting, and tissue assembly... - Disruption of acvrl1 increases endothelial cell number in zebrafish cranial vesselsBeth L Roman
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Development 129:3009-19. 2002..Movies available on-line.. - Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleasesAnkit Gupta
Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Nucleic Acids Res 39:381-92. 2011.... - Gateway compatible vectors for analysis of gene function in the zebrafishJacques A Villefranc
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Dev Dyn 236:3077-87. 2007..Taken together, this work provides an important platform for the rapid functional analyses of open reading frames in zebrafish embryos... - In vivo imaging of embryonic vascular development using transgenic zebrafishNathan D Lawson
Laboratory of Molecular Genetics, NICHD NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA
Dev Biol 248:307-18. 2002.... - Modulation of VEGF signalling output by the Notch pathwayArndt F Siekmann
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
Bioessays 30:303-13. 2008..In this review, we provide an overview of these biochemically distinct pathways and discuss how they may interact during endothelial cell differentiation and angiogenesis... - Targeted gene inactivation in zebrafish using engineered zinc-finger nucleasesXiangdong Meng
Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 6th Floor, 364 Plantation St, Worcester, Massachusetts 01605, USA
Nat Biotechnol 26:695-701. 2008..The use of engineered ZFNs to introduce heritable mutations into a genome obviates the need for embryonic stem cell lines and should be applicable to most animal species for which early-stage embryos are easily accessible... - Global analysis of hematopoietic and vascular endothelial gene expression by tissue specific microarray profiling in zebrafishLaurence Covassin
Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, Room 617, 364 Plantation Street, Worcester, MA 01605, USA
Dev Biol 299:551-62. 2006..Our results demonstrate that this is a robust approach for identification of cell type specific genes as well as for global analysis of cell type specific gene expression in zebrafish embryos... - Arteries and veins: making a difference with zebrafishNathan D Lawson
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Building 6B, Room 309, 6 Center Drive, Bethesda, Maryland 20892, USA
Nat Rev Genet 3:674-82. 2002.... - ChIPpeakAnno: a Bioconductor package to annotate ChIP-seq and ChIP-chip dataLihua J Zhu
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
BMC Bioinformatics 11:237. 2010..However, summarizing these tracks can be a daunting task, particularly if there are a large number of binding sites or the binding sites are distributed widely across the genome... - Notch signalling and the regulation of angiogenesisArndt F Siekmann
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Cell Adh Migr 1:104-6. 2007..In this overview, we discuss the emerging concepts elucidated by these studies and propose a model in which Notch acts reiteratively throughout the angiogenic process, likely by acting as a switch to determine a cell's response to Vegf... - The cilia protein IFT88 is required for spindle orientation in mitosisBenedicte Delaval
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Suite 210, Worcester, Massachusetts 01605, USA
Nat Cell Biol 13:461-8. 2011..This work identifies a mitotic mechanism for a cilia protein in the orientation of cell division and has important implications for the etiology of ciliopathies... - A nonsense mutation in zebrafish gata1 causes the bloodless phenotype in vlad tepesSusan E Lyons
National Human Genome Research Institute, National Institutes of Health, Building 49, Room 3A18, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:5454-9. 2002.... - An essential role for Fgfs in endodermal pouch formation influences later craniofacial skeletal patterningJustin Gage Crump
Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403 1254, USA
Development 131:5703-16. 2004..Moreover, we argue that the Fgf-dependent morphogenesis of the pharyngeal endoderm into pouches is critical for the later patterning of pharyngeal cartilages... - reg6 is required for branching morphogenesis during blood vessel regeneration in zebrafish caudal finsCheng-chen Huang
Department of Genetics, Washington University Medical School, St. Louis, MO 63110, USA
Dev Biol 264:263-74. 2003..Our results suggest that the reg6 mutation causes defects in branch formation and/or angiogenic sprouting... - Arteries define the position of the thyroid gland during its developmental relocalisationBurkhard Alt
Institute for Developmental Biology, University of Cologne, Gyrhofstrasse 17, Koln, Germany
Development 133:3797-804. 2006..Moreover, the involvement of vessels in thyroid relocalisation sheds new light on the interpretation of congenital thyroid defects in humans... - pak2a mutations cause cerebral hemorrhage in redhead zebrafishDavid A Buchner
Howard Hughes Medical Institute and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
Proc Natl Acad Sci U S A 104:13996-4001. 2007..These data provide in vivo evidence for a critical function of Pak2 in vascular integrity and demonstrate a severe disease phenotype resulting from loss of Pak2 function...
Research Grants
- Transcriptional Control of Endothelial DifferentiationNathan D Lawson; Fiscal Year: 2010..However, little is known about how these differences arise. In this proposal, we will utilize the zebra fish as a model system to identify gene programs that govern blood vessel identity in the developing embryo. ..
- Reverse Genetic Screening for Vascular Mutants Using Zinc Finger NucleasesNathan D Lawson; Fiscal Year: 2010..In this proposal, we will apply new technology to generate zebrafish knockouts in more than 30 genes to investigate their function during vascular development. ..
- Using zinc finger nucleases to manipulate the zebrafish genomeScot A Wolfe; Fiscal Year: 2010..We will apply this technology in zebrafish to better understand the processes involved in heart regeneration and blood vessel formation. ..
- Role of Notch in artery developmentNathan D Lawson; Fiscal Year: 2010..The studies in this proposal will address how blood vessel identities are determined during development. ..
- Role of Plcg1 in Vegf signalingNathan Lawson; Fiscal Year: 2009..Since these signaling mechanisms are evolutionary conserved, the molecules we identify in this proposal represent possible targets for clinical manipulation of pathological blood vessel formation. ..
- Transgenic screen for artery development mutantsNathan Lawson; Fiscal Year: 2007..Together, these studies will allow identification of genes that are required for proper artery and vein development and will represent putative targets for therapeutic manipulation. ..
- Identifying Vegf target genes during artery developmentNathan Lawson; Fiscal Year: 2005....