CHARLES LAUHON

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. ncbi request reprint The iscS gene in Escherichia coli is required for the biosynthesis of 4-thiouridine, thiamin, and NAD
    C T Lauhon
    University of Wisconsin School of Pharmacy, Madison, Wisconsin 53706, USA
    J Biol Chem 275:20096-103. 2000
  2. pmc Requirement for IscS in biosynthesis of all thionucleosides in Escherichia coli
    Charles T Lauhon
    School of Pharmacy, University of Wisconsin, Madison 53705, USA
    J Bacteriol 184:6820-9. 2002
  3. ncbi request reprint Substrate specificity for 4-thiouridine modification in Escherichia coli
    Charles T Lauhon
    School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53705, USA
    J Biol Chem 279:23022-9. 2004
  4. ncbi request reprint Substitutions in an active site loop of Escherichia coli IscS result in specific defects in Fe-S cluster and thionucleoside biosynthesis in vivo
    Charles T Lauhon
    School of Pharmacy, University of Wisconsin, Madison, WI 53705, USA
    J Biol Chem 279:19551-8. 2004
  5. ncbi request reprint Evidence for the transfer of sulfane sulfur from IscS to ThiI during the in vitro biosynthesis of 4-thiouridine in Escherichia coli tRNA
    R Kambampati
    School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 275:10727-30. 2000
  6. ncbi request reprint IscS is a sulfurtransferase for the in vitro biosynthesis of 4-thiouridine in Escherichia coli tRNA
    R Kambampati
    School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 38:16561-8. 1999
  7. pmc Lack of YggX results in chronic oxidative stress and uncovers subtle defects in Fe-S cluster metabolism in Salmonella enterica
    Elizabeth Skovran
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53726 4087, USA
    J Bacteriol 186:7626-34. 2004
  8. ncbi request reprint Functional diversity of the rhodanese homology domain: the Escherichia coli ybbB gene encodes a selenophosphate-dependent tRNA 2-selenouridine synthase
    Matt D Wolfe
    Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 8012, USA
    J Biol Chem 279:1801-9. 2004
  9. ncbi request reprint MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli
    Ravi Kambampati
    School of Pharmacy, University of Wisconsin, 777 Highland Avenue, Madison, Wisconsin 53706, USA
    Biochemistry 42:1109-17. 2003
  10. ncbi request reprint Orchestrating sulfur incorporation into RNA
    Charles T Lauhon
    Nat Chem Biol 2:182-3. 2006

Research Grants

  1. ENZYME MEDIATED THIOL MODIFICATION OF TRNA
    CHARLES LAUHON; Fiscal Year: 2002

Collaborators

Detail Information

Publications10

  1. ncbi request reprint The iscS gene in Escherichia coli is required for the biosynthesis of 4-thiouridine, thiamin, and NAD
    C T Lauhon
    University of Wisconsin School of Pharmacy, Madison, Wisconsin 53706, USA
    J Biol Chem 275:20096-103. 2000
    ..Thus, IscS plays a significant and specific role at the top of a potentially broad sulfur transfer cascade that is required for the biosynthesis of thiamin, NAD, Fe-S clusters, and thionucleosides...
  2. pmc Requirement for IscS in biosynthesis of all thionucleosides in Escherichia coli
    Charles T Lauhon
    School of Pharmacy, University of Wisconsin, Madison 53705, USA
    J Bacteriol 184:6820-9. 2002
    ..This may be due to a residual level of Fe-S cluster biosynthesis in iscS deletion strains. An overall scheme for thionucleoside biosynthesis in E. coli is discussed...
  3. ncbi request reprint Substrate specificity for 4-thiouridine modification in Escherichia coli
    Charles T Lauhon
    School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53705, USA
    J Biol Chem 279:23022-9. 2004
    ..However, the secondary and tertiary structure restrictions appear sufficient to explain why s4U modification is limited in the cell to tRNA...
  4. ncbi request reprint Substitutions in an active site loop of Escherichia coli IscS result in specific defects in Fe-S cluster and thionucleoside biosynthesis in vivo
    Charles T Lauhon
    School of Pharmacy, University of Wisconsin, Madison, WI 53705, USA
    J Biol Chem 279:19551-8. 2004
    ....
  5. ncbi request reprint Evidence for the transfer of sulfane sulfur from IscS to ThiI during the in vitro biosynthesis of 4-thiouridine in Escherichia coli tRNA
    R Kambampati
    School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 275:10727-30. 2000
    ..coli tRNA(Phe). Together, these results suggest that ThiI is a recipient of S(0) from IscS and catalyzes the ultimate sulfur transfer step in the biosynthesis of s(4)U...
  6. ncbi request reprint IscS is a sulfurtransferase for the in vitro biosynthesis of 4-thiouridine in Escherichia coli tRNA
    R Kambampati
    School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 38:16561-8. 1999
    ..Our identification of IscS as a tRNA sulfurtransferase provides support for this activity in vivo and further expands the role for NifS proteins as versatile sulfur-carrying enzymes...
  7. pmc Lack of YggX results in chronic oxidative stress and uncovers subtle defects in Fe-S cluster metabolism in Salmonella enterica
    Elizabeth Skovran
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53726 4087, USA
    J Bacteriol 186:7626-34. 2004
    ....
  8. ncbi request reprint Functional diversity of the rhodanese homology domain: the Escherichia coli ybbB gene encodes a selenophosphate-dependent tRNA 2-selenouridine synthase
    Matt D Wolfe
    Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 8012, USA
    J Biol Chem 279:1801-9. 2004
    ..These data are consistent with the ybbB gene encoding a tRNA 2-selenouridine synthase and identifies a new role for the rhodanese homology domain in enzymes...
  9. ncbi request reprint MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli
    Ravi Kambampati
    School of Pharmacy, University of Wisconsin, 777 Highland Avenue, Madison, Wisconsin 53706, USA
    Biochemistry 42:1109-17. 2003
    ....
  10. ncbi request reprint Orchestrating sulfur incorporation into RNA
    Charles T Lauhon
    Nat Chem Biol 2:182-3. 2006

Research Grants5

  1. ENZYME MEDIATED THIOL MODIFICATION OF TRNA
    CHARLES LAUHON; Fiscal Year: 2002
    ....