LESTER LAU

..Understanding the angiogenic actions of CCN1 and CCN2 may have implication in the development of therapeutic strategies targeting these proteins for the treatment of diseases such as cancer and fibrosis...

..These findings implicate CCN proteins as contextual regulators of the inflammatory response by dictating or enhancing the cytotoxicity of TNFalpha and related cytokines...

..Emerging studies suggest that CCN1 might be useful as a biomarker or therapeutic target in certain diseases...

..In addition, these findings suggest that Cyr61 might elicit disparate biological effects in different cell types through interaction with distinct integrin receptors...

..We propose a model in which Cyr61 integrates its activities on endothelial cells, fibroblasts, and macrophages to regulate the processes of angiogenesis, inflammation, and matrix remodeling in the context of cutaneous wound healing...

..Together, these findings establish CYR61 as a novel ligand for integrin alpha(v)beta(5) and show that CYR61 interacts with distinct integrins to mediate disparate activities in a cell type-specific manner...

..Taken together, these results identify Cyr61 as a novel player in chondrogenesis that contributes to the development of the mammalian embryonic skeleton...

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..Taken together, these results identify CYR61 as an angiogenic inducer that can promote tumor growth and vascularization; the results also suggest potential roles for CYR61 in physiologic and pathologic neovascularization...

..We show that hCYR61 acts in this cell system in part by displacement of bFGF bound to the extracellular matrix, thus increasing the effective concentration of bFGF. Possible biological implications of these observations are discussed...

..The possibility that integrin alphavbeta3 functions as a signaling receptor for Cyr61 accounts for most if not all activities that can be ascribed to Cyr61 to date and suggests a mechanism of action discussed herein...

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..We conclude that the cyr61 SRE functions as a serum response element and may account for the coordinate activation of cyr61 and c-fos...

..These properties of Xcyr61 provide a mechanism for integrating cell signalling, cell adhesion and cell migration during gastrulation...

..fr) as an important medium for the exchange of scientific knowledge and resources in the CCN field. Once more, the high quality of scientific communications and individual interactions set the stage for an extremely fruitful meeting...

..Cyr61 is synthesized maximally at 1 to 2 h after serum stimulation and has a short half-life within the cell...

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..We propose that different mechanisms can lead to the formation of such ternary complexes...

..These results suggest that the growth factor-inducible immediate early gene nur/77 encodes a ligand-binding protein that regulates the genomic response to growth factors...

..Using affinity-purified antibodies, we have identified the 3CH134 protein in serum-stimulated Balb/c 3T3 cells and determined that it has a short half-life...

..We have identified the Gly96 protein in Balb/c 3T3 cells using affinity-purified antibodies recognizing the Gly96 polypeptide. We show that Gly96 is glycosylated and has a short half-life in serum stimulated fibroblasts...

..Since 3CH134 expression is controlled transcriptionally and posttranscriptionally, it may represent a class of PTPases whose activity is regulated at the level of protein synthesis and degradation...

..This proposal is endorsed by the International CCN Society and will serve to eliminate confusion from the multiple names that have been given to these molecules...

..The mutant 3CH134 protein also forms a physical complex with the phosphorylated form of p42MAPK. These findings suggest that 3CH134 is a physiological MAP kinase phosphatase; we propose the name MKP-1 for this phosphatase...

..We hypothesize that Pip92 plays a role in mediating the cellular responses to a variety of extracellular signals...

..The jun-B protein, and perhaps other members of the jun family, may play a role in regulating the genomic response to growth factors...

..The SRE-like sequences could be responsible for the coordinate activation of zif/268 and fos after serum stimulation of 3T3 cells...

..mTF mRNA is present in many murine tissues and cell lines. Our results raise the possibility that mTF may also play a role in cell growth...

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..Through these studies we hope to elucidate the mechanism by which nucleolar surveillance functions as a novel cell cycle checkpoint. ..

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..Together, these studies will provide novel insights into how CCN proteins regulate cardiovascular development through extracellular matrix signaling, and shed light on their roles in cardiovascular diseases. ..

..Through these studies, we hope to gain insight into how CCN1 acts as a matricellular protein to control multiple cellular processes. ..

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..Together, these studies will provide novel insights into how CCN proteins regulate cardiovascular development through extracellular matrix signaling, and shed light on their roles in cardiovascular diseases. ..

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..Through these studies, a better understanding of how Cyr61 can function in such diverse yet important biological processes may emerge. ..

..Third, the role of Cyr61 in tumor growth and metastasis will be evaluated, and intervention of Cyr61 activities will be explored as a means of restricting tumor growth. ..

..The effects of constitutive expression of this gene, inactivation by anti-sense RNA, and expression during phases of the cell cycle when it is usually silent will be investigated using a number of recombinant constructs...

..Then we will explore Cyr61 as a potential therapeutic target. We will test whether blockade of Cyr6l function, using monoclonal antibodies or inhibitory peptides, can prevent or ameliorate arthritis and joint destruction. ..