Lisa M Larkin

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint Functional evaluation of nerve-skeletal muscle constructs engineered in vitro
    Lisa M Larkin
    Department of Biomedical Engineering, Division of Geriatric Medicine, Muscle Mechanics Laboratory, Institute of Gerontology, University of Michigan, Ann Arbor, Michigan 48109 2007, USA
    In Vitro Cell Dev Biol Anim 42:75-82. 2006
  2. pmc Synergist muscle ablation and recovery from nerve-repair grafting: contractile and metabolic function
    L M Larkin
    Division of Geriatric Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Appl Physiol (1985) 89:1469-76. 2000
  3. pmc Effect of aging and obesity on insulin responsiveness and glut-4 glucose transporter content in skeletal muscle of Fischer 344 x Brown Norway rats
    L M Larkin
    Division of Geriatric Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor 48109 0940, USA
    J Gerontol A Biol Sci Med Sci 56:B486-92. 2001
  4. pmc Effects of age and nerve-repair grafts on reinnervation and fiber type distribution of rat medial gastrocnemius muscles
    Lisa M Larkin
    Department of Internal Medicine, Division of Geriatric Medicine, University of Michigan, Ann Arbor 48109 2007, USA
    Mech Ageing Dev 124:653-61. 2003
  5. pmc The effect of cleft palate repair on contractile properties of single permeabilized muscle fibers from congenitally cleft goat palates
    Michael C Hanes
    Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Ann Plast Surg 60:188-93. 2008
  6. pmc Effect of cleft palate repair on the susceptibility to contraction-induced injury of single permeabilized muscle fibers from congenitally-clefted goat palates
    Erik P Rader
    Department of Surgery, University of Michigan, Ann Arbor, MI, USA
    Cleft Palate Craniofac J 45:113-20. 2008
  7. pmc Contractile properties of single permeabilized muscle fibers from congenital cleft palates and normal palates of Spanish goats
    Michael C Hanes
    Section of Plastic Surgery, Division of Geriatric Medicine, and Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109 2007, USA
    Plast Reconstr Surg 119:1685-94. 2007
  8. pmc Skeletal muscle weakness due to deficiency of CuZn-superoxide dismutase is associated with loss of functional innervation
    Lisa M Larkin
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109 2200, USA
    Am J Physiol Regul Integr Comp Physiol 301:R1400-7. 2011
  9. pmc Three-dimensional engineered bone-ligament-bone constructs for anterior cruciate ligament replacement
    Jinjin Ma
    Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA
    Tissue Eng Part A 18:103-16. 2012
  10. ncbi request reprint Characterization of skeletal muscle effects associated with daptomycin in rats
    Tatiana Y Kostrominova
    Department of Molecular and Integrative Physiology, BSRB, University of Michigan, Ann Arbor, Michigan, USA
    Muscle Nerve 42:385-93. 2010

Collaborators

Detail Information

Publications31

  1. ncbi request reprint Functional evaluation of nerve-skeletal muscle constructs engineered in vitro
    Lisa M Larkin
    Department of Biomedical Engineering, Division of Geriatric Medicine, Muscle Mechanics Laboratory, Institute of Gerontology, University of Michigan, Ann Arbor, Michigan 48109 2007, USA
    In Vitro Cell Dev Biol Anim 42:75-82. 2006
    ..In conclusion, we have successfully engineered a 3-D nerve-muscle construct that displays functional neuromuscular junctions and can be electrically stimulated to contract via the neural extensions projecting from the construct...
  2. pmc Synergist muscle ablation and recovery from nerve-repair grafting: contractile and metabolic function
    L M Larkin
    Division of Geriatric Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Appl Physiol (1985) 89:1469-76. 2000
    ..The enhanced endurance after synergist ablation was accompanied by increased GLUT-4 protein, suggesting a role for increased uptake of circulating glucose during contraction...
  3. pmc Effect of aging and obesity on insulin responsiveness and glut-4 glucose transporter content in skeletal muscle of Fischer 344 x Brown Norway rats
    L M Larkin
    Division of Geriatric Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor 48109 0940, USA
    J Gerontol A Biol Sci Med Sci 56:B486-92. 2001
    ....
  4. pmc Effects of age and nerve-repair grafts on reinnervation and fiber type distribution of rat medial gastrocnemius muscles
    Lisa M Larkin
    Department of Internal Medicine, Division of Geriatric Medicine, University of Michigan, Ann Arbor 48109 2007, USA
    Mech Ageing Dev 124:653-61. 2003
    ..Thus, we conclude that diminished contractile function of aged and/or nerve-repair grafted MG muscle may be explained, in part, by an increase in the percentage of denervated fibers...
  5. pmc The effect of cleft palate repair on contractile properties of single permeabilized muscle fibers from congenitally cleft goat palates
    Michael C Hanes
    Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Ann Plast Surg 60:188-93. 2008
    ..No differences in sF(0) were observed. nP(max) increased with presence of type 2 fibers. The persistent state of type 2 fibers following palatoplasty leads to increased fatigue in the LVP of MRP subjects and may cause VPI symptoms...
  6. pmc Effect of cleft palate repair on the susceptibility to contraction-induced injury of single permeabilized muscle fibers from congenitally-clefted goat palates
    Erik P Rader
    Department of Surgery, University of Michigan, Ann Arbor, MI, USA
    Cleft Palate Craniofac J 45:113-20. 2008
    ..As an initial step to determining the validity of this theory, we tested the hypothesis that, in most cases, repair induces the transformation to type 1 fibers, thus diminishing susceptibility to injury...
  7. pmc Contractile properties of single permeabilized muscle fibers from congenital cleft palates and normal palates of Spanish goats
    Michael C Hanes
    Section of Plastic Surgery, Division of Geriatric Medicine, and Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109 2007, USA
    Plast Reconstr Surg 119:1685-94. 2007
    ..The authors hypothesized that levator veli palatini muscle dysfunction inherent to cleft palates could affect the timing and outcome of cleft palate repair...
  8. pmc Skeletal muscle weakness due to deficiency of CuZn-superoxide dismutase is associated with loss of functional innervation
    Lisa M Larkin
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109 2200, USA
    Am J Physiol Regul Integr Comp Physiol 301:R1400-7. 2011
    ..Moreover, the findings support using Sod1(-/-) mice to explore mechanistic links between oxidative stress and the progression of deficits in muscle structure and function...
  9. pmc Three-dimensional engineered bone-ligament-bone constructs for anterior cruciate ligament replacement
    Jinjin Ma
    Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA
    Tissue Eng Part A 18:103-16. 2012
    ..These results suggest that our constructs after implantation can obtain physiologically relevant structural and functional characteristics comparable to those of adult ACL. They present a viable option for ACL replacement...
  10. ncbi request reprint Characterization of skeletal muscle effects associated with daptomycin in rats
    Tatiana Y Kostrominova
    Department of Molecular and Integrative Physiology, BSRB, University of Michigan, Ann Arbor, Michigan, USA
    Muscle Nerve 42:385-93. 2010
    ..Some fibers were Evans Blue Dye-positive but were not yet infiltrated by neutrophils. This suggests that the sarcolemma may be the primary target for the observed effects...
  11. pmc Weakness of whole muscles in mice deficient in Cu, Zn superoxide dismutase is not explained by defects at the level of the contractile apparatus
    Lisa M Larkin
    Departments of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Age (Dordr) 35:1173-81. 2013
    ..We conclude that the low specific F o of muscles of Sod1 (-/-) mice is not explained by damage to contractile proteins. Moreover, the properties of fibers of Sod1 (-/-) mice do not recapitulate those observed with aging in WT animals...
  12. pmc Ultrastructure of myotendinous junctions in tendon-skeletal muscle constructs engineered in vitro
    Tatiana Y Kostrominova
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA
    Histol Histopathol 24:541-50. 2009
    ..The muscle-FRT construct model could be used for studies of developmental mechanisms involved in the establishment of interfaces among all four muscular-skeletal tissues: muscle, tendon and cartilage/bone...
  13. pmc Effects of aerobic exercise training on the protein kinase B (PKB)/mammalian target of rapamycin (mTOR) signaling pathway in aged skeletal muscle
    Thomas H Reynolds
    Department of Internal Medicine, Division of Geriatric Medicine, University of Michigan and the GRECC, Ann Arbor Veterans Affairs Healthcare System, Ann Arbor, MI, USA
    Exp Gerontol 39:379-85. 2004
    ..001) These findings indicate the aerobic exercise training may attenuate the age-related decline in protein synthesis, a process that appears to be due, in part, to increases in mTOR and PKB...
  14. doi request reprint The effect of implantation on scaffoldless three-dimensional engineered bone constructs
    Michael J Smietana
    Biomedical Engineering, University of Michigan, 2025 BSRB, Ann Arbor, MI 48109 2200, USA
    In Vitro Cell Dev Biol Anim 45:512-22. 2009
    ..Our data suggest that BMSC can be successfully used for the generation of scaffoldless EBC, and this model can be potentially used for the generation of autologous bone transplants in humans...
  15. ncbi request reprint Glucose transporter content and glucose uptake in skeletal muscle constructs engineered in vitro
    Erin L Baker
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109 2007, USA
    In Vitro Cell Dev Biol Anim 39:434-9. 2003
    ..3-fold greater than GLUT-4 protein content, suggesting that the glucose transport of the engineered myooids is similar in several respects to that observed in both fetal and denervated skeletal muscle tissue...
  16. pmc Reactive oxygen species on bone mineral density and mechanics in Cu,Zn superoxide dismutase (Sod1) knockout mice
    Michael J Smietana
    Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109 2200, USA
    Biochem Biophys Res Commun 403:149-53. 2010
    ....
  17. pmc TGF-β1 enhances contractility in engineered skeletal muscle
    Michael R Weist
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109 2200, USA
    J Tissue Eng Regen Med 7:562-71. 2013
    ..0 ng/ml TGF-β1 versus 0, 0.5 and 1.0 ng/ml. The addition of 2.0 ng/ml TGF-β1 to the culture medium of engineered 3D skeletal muscle constructs enhanced contractility and extracellular matrix organization...
  18. pmc Structure and functional evaluation of tendon-skeletal muscle constructs engineered in vitro
    Lisa M Larkin
    Department of Biomedical Engineering, Division of Geriatric Medicine, Muscle Mechanics Laboratory, University of Michigan, Ann Arbor, Michigan 48109, USA
    Tissue Eng 12:3149-58. 2006
    ..In conclusion, using 3 sources of tendon tissue, we successfully engineered 3-D muscle-tendon constructs with functionally viable MTJ, characterized by structural features and protein expression patterns resembling neonatal MTJs in vivo...
  19. pmc Effect of implantation on engineered skeletal muscle constructs
    Michael L Williams
    Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA
    J Tissue Eng Regen Med 7:434-42. 2013
    ..However, in terms of force production, the muscle constructs need longer implantation times to fully develop an adult phenotype...
  20. doi request reprint Three-dimensional engineered bone from bone marrow stromal cells and their autogenous extracellular matrix
    Fatima N Syed-Picard
    Department of Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA
    Tissue Eng Part A 15:187-95. 2009
    ..The resulting engineered tissues are the first 3D bone tissues developed without the use of exogenous scaffolding...
  21. ncbi request reprint Age-related changes in the structure and function of skeletal muscles
    John A Faulkner
    Molecular and Integrative Physiology, and Biomedical Engineering, Biomedical Science Research Building, University of Michigan, Ann Arbor, Michigan, USA
    Clin Exp Pharmacol Physiol 34:1091-6. 2007
    ....
  22. pmc Effects of high- and low-velocity resistance training on the contractile properties of skeletal muscle fibers from young and older humans
    Dennis R Claflin
    Department of Surgery, Section of Plastic Surgery, Univ of Michigan, BSRB, Rm 2023, 109 Zina Pitcher Place, Ann Arbor, MI 48109 2200, USA
    J Appl Physiol (1985) 111:1021-30. 2011
    ..5 times higher against reduced loads is equally effective for eliciting an adaptive response in type 2 fibers from human skeletal muscle...
  23. pmc Development of a scaffoldless three-dimensional engineered nerve using a nerve-fibroblast co-culture
    Jennifer Baltich
    Biomedical Engineering, University of Michigan, Room 2025 Biomedical Science Research Building BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109 2200, USA
    In Vitro Cell Dev Biol Anim 46:438-44. 2010
    ..The presence of Schwann cells and the ability to conduct neuronal signals in vitro suggest the scaffoldless 3D nerve constructs will be a viable option for nerve repair...
  24. doi request reprint Effect of daptomycin on primary rat muscle cell cultures in vitro
    Tatiana Y Kostrominova
    Department of Molecular and Integrative Physiology, BSRB, University of Michigan, Ann Arbor, MI 48109 2200, USA
    In Vitro Cell Dev Biol Anim 46:613-8. 2010
    ..These results suggest that the putative targets for daptomycin effects on skeletal muscle are structures on the plasma membrane of highly differentiated myotubes...
  25. pmc Repeated bouts of aerobic exercise lead to reductions in skeletal muscle free radical generation and nuclear factor kappaB activation
    Susan V Brooks
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA
    J Physiol 586:3979-90. 2008
    ....
  26. pmc Effect of age on skeletal muscle proteolysis in extensor digitorum longus muscles of B6C3F1 mice
    Thomas H Reynolds
    Division of Geriatric Medicine, University of Michigan, and Veteran Affairs Ann Arbor Health Care System, Ann Arbor, USA
    J Gerontol A Biol Sci Med Sci 57:B198-201. 2002
    ..04). An inverse relationship existed between muscle mass and protein degradation (r = -.67; p =.0001). We conclude that skeletal muscle protein degradation rates decrease with maturation and increase with advancing age...
  27. pmc Chemical versus dual energy x-ray absorptiometry for detecting age-associated body compositional changes in male rats
    R S Feely
    Geriatric Research, Education, and Clinical Center, Department of Veterans Affairs Medical Center, University of Michigan, CCGCB, 5302, 1500 East Medical Center Drive, Ann Arbor, MI 48109 0940, USA
    Exp Gerontol 35:417-27. 2000
    ..97, P < 0.0001), but less strong for FFM (r = 0.59, P = 0.0002). In conclusion, compared to CHEM, DXA consistently overestimated CM and FM across the age groups by 9% and 77%, respectively, and underestimated FFM by 5%...
  28. pmc Transient enhancement of GLUT-4 levels in rat epitrochlearis muscle after exercise training
    T H Reynolds
    Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Appl Physiol (1985) 88:2240-5. 2000
    ..We believe that the transient enhancement in GLUT-4 protein after exercise training is due to a short GLUT-4 half-life, a process that is primarily regulated by pretranslational mechanisms...
  29. pmc Effect of aging on rat skeletal muscle beta-AR function in male Fischer 344 x brown Norway rats
    L M Larkin
    Department of Internal Medicine, and Institute of Gerontology, University of Michigan, Ann Arbor, USA
    Am J Physiol 270:R462-8. 1996
    ..In addition, there was an age-associated increase in skeletal muscle beta-AR density, whereas in skeletal muscle beta-AR-stimulated AC activity remained unchanged with age...
  30. pmc Contraction-induced injury to single permeabilized muscle fibers from normal and congenitally-clefted goat palates
    Erik P Rader
    Department of Surgery, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Cleft Palate Craniofac J 44:216-22. 2007
    ..We tested the hypothesis that, compared with single permeabilized levator veli palatini muscle fibers from normal palates of adult goats, those from cleft palates are more susceptible to lengthening contraction-induced injury...
  31. ncbi request reprint Engineering muscle constructs for the creation of functional engineered musculoskeletal tissue
    Jacob P Mertens
    Molecular and Integrative Physiology, University of Michigan, MI, USA
    Regen Med 9:89-100. 2014
    ..However, recent advances in tissue engineering using synthetic scaffolds, native scaffolds, or scaffold-free approaches may lead to a solution for repair of VML injuries. ..

Research Grants8

  1. Electromechanical Stimuli on the Development of Engineered Myotendinous Junction
    Lisa Larkin; Fiscal Year: 2007
    ..We also propose to use bioreactors to place the tendon-muscle constructs into physical environments which simulate the stress, strain, and contractile activity of hind limb muscles resembling those found in vivo. ..
  2. Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
    Lisa Larkin; Fiscal Year: 2007
    ..Specific Aim 4. To evaluate the effect of an in vivo implantation into a host animal on the structure and function of the nerve-muscle tendon construct at four weeks. ..
  3. Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
    Lisa Larkin; Fiscal Year: 2009
    ..Specific Aim 4. To evaluate the effect of an in vivo implantation into a host animal on the structure and function of the nerve-muscle tendon construct at four weeks. ..
  4. Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
    Lisa M Larkin; Fiscal Year: 2010
    ..Specific Aim 4. To evaluate the effect of an in vivo implantation into a host animal on the structure and function of the nerve-muscle tendon construct at four weeks. ..
  5. Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
    Lisa Larkin; Fiscal Year: 2009
    ..Specific Aim 4. To evaluate the effect of an in vivo implantation into a host animal on the structure and function of the nerve-muscle tendon construct at four weeks. ..
  6. Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
    Lisa M Larkin; Fiscal Year: 2010
    ..Specific Aim 4. To evaluate the effect of an in vivo implantation into a host animal on the structure and function of the nerve-muscle tendon construct at four weeks. ..