DAVID ANDREW contact LARGAESPADA

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. pmc TAPDANCE: an automated tool to identify and annotate transposon insertion CISs and associations between CISs from next generation sequence data
    Aaron L Sarver
    Biostatistics and Bioinformatics Masonic Cancer Center, University of Minnesota, Minneapolis, USA
    BMC Bioinformatics 13:154. 2012
  2. pmc Interweaving the strands: β-catenin, an HIV co-receptor, and Schwann cell tumors
    DAVID LARGAESPADA
    Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
    Cancer Cell 23:269-71. 2013
  3. pmc Review: In vivo models for defining molecular subtypes of the primitive neuroectodermal tumor genome: current challenges and solutions
    Jon D Larson
    The Center For Genome Engineering and Masonic Cancer Center, University of Minnesota, 6 160 Jackson Hall, 321 Church Street South East, Minneapolis, MN 55455, USA
    In Vivo 26:487-500. 2012
  4. pmc Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon
    Aron M Geurts
    Department of Genetics, Cell Biology, and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    BMC Biotechnol 6:30. 2006
  5. pmc Transposon-mediated mutagenesis of somatic cells in the mouse for cancer gene identification
    David A Largaespada
    Masonic Cancer Center University of Minnesota, Department of Genetics, Arnold and Mabel Beckman Center for Genome Engineering, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
    Methods 49:282-6. 2009
  6. ncbi request reprint Combinatorial antiangiogenic gene therapy by nonviral gene transfer using the sleeping beauty transposon causes tumor regression and improves survival in mice bearing intracranial human glioblastoma
    John R Ohlfest
    Department of Neurosurgery, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 12:778-88. 2005
  7. ncbi request reprint Hopping around the tumor genome: transposons for cancer gene discovery
    Lara S Collier
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, The Cancer Center, University of Minnesota Twin Cities, Minneapolis, Minnesota 55455, USA
    Cancer Res 65:9607-10. 2005
  8. pmc Transposon-mediated mutagenesis in somatic cells: identification of transposon-genomic DNA junctions
    David A Largaespada
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN
    Methods Mol Biol 435:95-108. 2008
  9. ncbi request reprint Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression
    Paul R Score
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:617-24. 2006
  10. ncbi request reprint RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissues
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:625-30. 2006

Research Grants

  1. A Genetic Screen for HCC Genes
    DAVID ANDREW contact LARGAESPADA; Fiscal Year: 2010
  2. Transposon-based screens for colorectal cancer genes
    DAVID ANDREW contact LARGAESPADA; Fiscal Year: 2010
  3. Sleeping Beauty for uncovering cancer genes in mice
    DAVID ANDREW LARGAESPADA; Fiscal Year: 2010
  4. Transposon-based somatic mutagenesis/prostate cancer genetics
    DAVID LARGAESPADA; Fiscal Year: 2007
  5. Sleeping Beauty for uncovering cancer genes in mice
    DAVID LARGAESPADA; Fiscal Year: 2007
  6. Transposon Mediated Insertional Mutagenesis in Mice
    DAVID LARGAESPADA; Fiscal Year: 2003
  7. COOPERATING GENETIC EVENTS IN MYELOID LEUKEMIA DEVELOPME
    DAVID LARGAESPADA; Fiscal Year: 2002
  8. NF1 GENE IN MYELOID LEUKEMIA AND CYTOKINE SIGNALING
    DAVID LARGAESPADA; Fiscal Year: 2002
  9. A Genetic Screen for HCC Genes
    Adam J Dupuy; Fiscal Year: 2011

Collaborators

Detail Information

Publications41

  1. pmc TAPDANCE: an automated tool to identify and annotate transposon insertion CISs and associations between CISs from next generation sequence data
    Aaron L Sarver
    Biostatistics and Bioinformatics Masonic Cancer Center, University of Minnesota, Minneapolis, USA
    BMC Bioinformatics 13:154. 2012
    ..Previous approaches applied to this problem also required significant manual annotation...
  2. pmc Interweaving the strands: β-catenin, an HIV co-receptor, and Schwann cell tumors
    DAVID LARGAESPADA
    Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
    Cancer Cell 23:269-71. 2013
    ..Together with the availability of small molecules targeting CXCR4, this finding suggests new avenues for cancer therapy...
  3. pmc Review: In vivo models for defining molecular subtypes of the primitive neuroectodermal tumor genome: current challenges and solutions
    Jon D Larson
    The Center For Genome Engineering and Masonic Cancer Center, University of Minnesota, 6 160 Jackson Hall, 321 Church Street South East, Minneapolis, MN 55455, USA
    In Vivo 26:487-500. 2012
    ....
  4. pmc Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon
    Aron M Geurts
    Department of Genetics, Cell Biology, and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    BMC Biotechnol 6:30. 2006
    ..We sought to generate a transposon system that would be capable of reporting the expression pattern of a mouse gene while allowing for conditional expression of a gene of interest in a tissue- or temporal-specific pattern...
  5. pmc Transposon-mediated mutagenesis of somatic cells in the mouse for cancer gene identification
    David A Largaespada
    Masonic Cancer Center University of Minnesota, Department of Genetics, Arnold and Mabel Beckman Center for Genome Engineering, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
    Methods 49:282-6. 2009
    ..This article describes the strains useful for SB-based screens for cancer genes in mice and how they are deployed in an experiment...
  6. ncbi request reprint Combinatorial antiangiogenic gene therapy by nonviral gene transfer using the sleeping beauty transposon causes tumor regression and improves survival in mice bearing intracranial human glioblastoma
    John R Ohlfest
    Department of Neurosurgery, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 12:778-88. 2005
    ..We show that SB can be used to increase animal survival significantly (P = 0.008) by combinatorial antiangiogenic gene transfer in an intracranial glioma model...
  7. ncbi request reprint Hopping around the tumor genome: transposons for cancer gene discovery
    Lara S Collier
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, The Cancer Center, University of Minnesota Twin Cities, Minneapolis, Minnesota 55455, USA
    Cancer Res 65:9607-10. 2005
    ..These results indicate that the SB system has great potential both for generating specific mouse models of human cancer and for cancer gene discovery in a wide variety of tissues...
  8. pmc Transposon-mediated mutagenesis in somatic cells: identification of transposon-genomic DNA junctions
    David A Largaespada
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN
    Methods Mol Biol 435:95-108. 2008
    ..We provide an overview of some general issues related to use of Sleeping Beauty for cancer genetic studies and present here the polymerase chain reaction-based method for cloning transposon-tagged sequences from tumors...
  9. ncbi request reprint Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression
    Paul R Score
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:617-24. 2006
    ..The results also provide additional evidence for the effectiveness of the Sleeping Beauty transposon system as an in vivo DNA-mediated gene transfer strategy for achieving long-term expression...
  10. ncbi request reprint RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissues
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:625-30. 2006
    ..We conclude that in vitro-transcribed mRNA can be used as an effective source of transposase for SB-mediated transposition in mammalian cells and tissues...
  11. ncbi request reprint Nonviral vectors for cancer gene therapy: prospects for integrating vectors and combination therapies
    John R Ohlfest
    Department of Neurosurgery, University of Minnesota, Minneapolis, MN 55455, USA
    Curr Gene Ther 5:629-41. 2005
    ..This article reviews the current status of nonviral cancer gene therapy, with an emphasis on combination strategies, long-term gene transfer using transposons and bacteriophage integrases, and future directions...
  12. pmc Transposable elements and the dynamic somatic genome
    Lara S Collier
    The Department of Genetics, Cell Biology and Development, The Cancer Center, The University of Minnesota Twin Cities, Church St SE, Minneapolis, Minnesota 55455, USA
    Genome Biol 8:S5. 2007
    ..This review outlines the ways in which transposable elements have important consequences for somatic cell genomes...
  13. ncbi request reprint PCR-based procedures to isolate insertion sites of DNA elements
    Bin Yin
    University of Minnesota, Minneapolis, MN, USA
    Biotechniques 43:79-84. 2007
    ....
  14. ncbi request reprint DNA methylation of Sleeping Beauty with transposition into the mouse genome
    Chang Won Park
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Genes Cells 10:763-76. 2005
    ..This may depend on a number of different factors including the cargo DNA sequence, chromosomal context of the insertion site, and/or host genetic background...
  15. ncbi request reprint Sleeping beauty transposon-mediated gene therapy for prolonged expression
    Perry B Hackett
    Department of Genetics, Cell Biology and Development Arnold and Mabel Beckman Center for Transposon Research University of Minnesota Minneapolis, Minnesota 55455, USA
    Adv Genet 54:189-232. 2005
    ..Here we review the activities of the SB system in mice used as a model for human gene therapy, methods of delivery of the SB system, and its efficacy in ameliorating disorders that model human disease...
  16. ncbi request reprint Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse
    Lara S Collier
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, The Cancer Center, The University of Minnesota Twin Cities, Minneapolis, Minnesota 55455, USA
    Nature 436:272-6. 2005
    ..Sleeping Beauty is a new tool for unbiased, forward genetic screens for cancer genes in vivo...
  17. pmc Mammalian germ-line transgenesis by transposition
    Adam J Dupuy
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 99:4495-9. 2002
    ..We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse...
  18. pmc MTID: a database of Sleeping Beauty transposon insertions in mice
    Kevin Roberg-Perez
    University of Minnesota Cancer Center, Minneapolis, MN 55455, USA
    Nucleic Acids Res 31:78-81. 2003
    ..The flanking sequence used to map the insertion is also provided. Researchers will be able to identify insertions of interest and request mice or frozen sperm that carry the insertion...
  19. ncbi request reprint Gene transfer into genomes of human cells by the sleeping beauty transposon system
    Aron M Geurts
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:108-17. 2003
    ..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy...
  20. ncbi request reprint A bad rap: Rap1 signaling and oncogenesis
    David A Largaespada
    University of Minnesota Cancer Center, 6 160 Jackson Hall, 321 Church Street S E, Minneapolis, MN 55455, USA
    Cancer Cell 4:3-4. 2003
    ..The authors present additional data showing that the level of Rap1 activation is important for regulating myelopoiesis and that, in the right context, can deliver an oncogenic signal...
  21. pmc Transposon mutagenesis of the mouse germline
    Corey M Carlson
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis 55455, USA
    Genetics 165:243-56. 2003
    ..Finally, we characterize an embryonic lethal mutation caused by endogenous splicing disruption in mice carrying a particular intron-inserted gene-trap transposon...
  22. pmc Generating and manipulating transgenic animals using transposable elements
    David A Largaespada
    Department of Genetics, Cell Biology and Development, University of Minnesota Cancer Center, Minneapolis, MN 55455, USA
    Reprod Biol Endocrinol 1:80. 2003
    ..General considerations and predictions about the future utility of transposon technology are discussed...
  23. ncbi request reprint Integration and long-term expression in xenografted human glioblastoma cells using a plasmid-based transposon system
    John R Ohlfest
    Department of Genetics, Cell Biology, and Development, University of Minnesota, and University of Minnesota Cancer Center, Arnold and Mabel Beckman Center for Transposon Research, Minneapolis, MN 55455, USA
    Mol Ther 10:260-8. 2004
    ..SB-mediated intratumoral gene transfer is a novel, nonviral technique that could be used to augment conventional therapy for glioblastoma or other cancers...
  24. ncbi request reprint Insertional mutagenesis in mice: new perspectives and tools
    Corey M Carlson
    Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, Minneapolis, Minnesota 55455, USA
    Nat Rev Genet 6:568-80. 2005
    ..These new technologies will greatly contribute to the speed and ease with which we achieve complete functional annotation of the mouse genome...
  25. ncbi request reprint Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system
    Adam J Dupuy
    National Cancer Institute, Center for Cancer Research, Frederick, Maryland 21702, USA
    Nature 436:221-6. 2005
    ..The uses of SB are also not restricted to the mouse and could potentially be used for forward genetic screens in any higher eukaryote in which transgenesis is possible...
  26. ncbi request reprint Phenotypic correction and long-term expression of factor VIII in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon system
    John R Ohlfest
    Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, 6 160 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
    Blood 105:2691-8. 2005
    ..Thus, SB provides a nonviral means for sustained FVIII gene delivery in a mouse model of hemophilia A if the immune response is prevented...
  27. doi request reprint A facile method for somatic, lifelong manipulation of multiple genes in the mouse liver
    Kirk J Wangensteen
    The Arnold and Mabel Beckman Center for Transposon Research, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
    Hepatology 47:1714-24. 2008
    ..Conclusion: Coexpression with FAH is an effective technique for lifelong expression of transgenes in adult hepatocytes with applicability to a wide variety of genetic studies in the liver...
  28. pmc The GAP-related domain of neurofibromin attenuates proliferation and downregulates N- and K-Ras activation in Nf1-negative AML cells
    Kelly J Morgan
    University of Minnesota, Department of Genetics, Cell Biology and Development, University of Minnesota Cancer Center, Minneapolis, MN, USA
    Leuk Res 31:1107-13. 2007
    ....
  29. pmc Transposons for cancer gene discovery: Sleeping Beauty and beyond
    Lara S Collier
    The Department of Genetics, Cell Biology and Development, The Cancer Center, The University of Minnesota Twin Cities, Church St SE, Minneapolis, Minnesota 55455, USA
    Genome Biol 8:S15. 2007
    ..Here, we discuss the future of Sleeping Beauty for cancer genetic studies and the potential use of additional transposable elements for somatic mutagenesis...
  30. pmc Harnessing a high cargo-capacity transposon for genetic applications in vertebrates
    Darius Balciunas
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS Genet 2:e169. 2006
    ..Together, Tol2 provides a novel nonviral vector for the delivery of large genetic payloads for gene therapy and other transgenic applications...
  31. ncbi request reprint Gene insertion and long-term expression in lung mediated by the Sleeping Beauty transposon system
    Lalitha R Belur
    Gene Therapy Program, Institute of Human Genetics, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:501-7. 2003
    ..We used a linker-mediated PCR technique to recover transposon flanking sequences, demonstrating transposition of pT/L into mouse chromosomal DNA of the lung...
  32. ncbi request reprint Activation of clg, a novel dbl family guanine nucleotide exchange factor gene, by proviral insertion at evi24, a common integration site in B cell and myeloid leukemias
    Karen L Himmel
    University of Minnesota Cancer Center, Institute of Human Genetics, Department of Genetics, University of Minnesota, Minneapolis 55455, USA
    J Biol Chem 277:13463-72. 2002
    ..This study and studies showing that the human homolog of EVI24 is located in a region of 19q13 frequently amplified in B cell lymphomas and pancreatic and breast cancers implicate Clg and Cdc42 activation in mouse and human cancers...
  33. ncbi request reprint Target-site preferences of Sleeping Beauty transposons
    Geyi Liu
    Department of Genetics, Cell Biology, and Development and The Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 346:161-73. 2005
    ..Our observations suggest that particular deformations of the double helix predicted by the V(step) algorithm can distinguish TA sites that vary by about 16-fold in their preferences for accommodating insertions of SB transposons...
  34. pmc Somatic integration of an oncogene-harboring Sleeping Beauty transposon models liver tumor development in the mouse
    Corey M Carlson
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 102:17059-64. 2005
    ..This system can be used to rapidly model tumors caused by defined genetic changes...
  35. ncbi request reprint Cancer gene discovery using the Sleeping Beauty transposon
    Timothy K Starr
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, The Cancer Center, The University of Minnesota, Twin Cities, Minneapolis, Minnesota, USA
    Cell Cycle 4:1744-8. 2005
    ..In this article we discuss the history of cancer gene discovery and propose novel forward genetic screens using Sleeping Beauty transposon aimed at specific tissues and accelerating the discovery of recessive tumor suppressor genes...
  36. ncbi request reprint Transforming science: cancer gene identification
    Lara S Collier
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota Twin Cities, 6 160 Jackson Hall, 321 Church Street South East, Minneapolis, MN 55455, USA
    Curr Opin Genet Dev 16:23-9. 2006
    ....
  37. pmc Structure-based prediction of insertion-site preferences of transposons into chromosomes
    Aron M Geurts
    Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    Nucleic Acids Res 34:2803-11. 2006
    ..ProTIS analysis can be useful for functional genomic, gene transfer and human gene therapy applications using the SB system...
  38. pmc Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers
    Aron M Geurts
    Department of Genetics, Cell Biology, and Development, University of Minnesota Twin Cities, Minneapolis, Minnesota, United States of America
    PLoS Genet 2:e156. 2006
    ..Additionally, these mice may serve as a model for chromosome rearrangements caused by transposable elements during the evolution of vertebrate genomes...
  39. pmc Beta common receptor inactivation attenuates myeloproliferative disease in Nf1 mutant mice
    Andrew Kim
    Department of Pediatrics, University of California San Francisco, USA
    Blood 109:1687-91. 2007
    ..Whereas inhibiting GM-CSF signaling may be of therapeutic benefit in JMML, our data also demonstrate aberrant proliferation of Nf1-/-myeloid progenitors that is independent of signaling through the GM-CSF receptor...
  40. ncbi request reprint Genetically modified mice in cancer research
    David A Largaespada
    Mouse Genetics Laboratory, Department of Genetics, Cell Biology and Development, University of Minnesota Cancer Center, Minneapolis, MN, USA
    Methods Mol Biol 209:311-32. 2003

Research Grants17

  1. A Genetic Screen for HCC Genes
    DAVID ANDREW contact LARGAESPADA; Fiscal Year: 2010
    ..We will discover what genes, when damaged, can cause these tumors. This will help us decide how best to treat this dangerous forms of cancer. ..
  2. Transposon-based screens for colorectal cancer genes
    DAVID ANDREW contact LARGAESPADA; Fiscal Year: 2010
    ..We will discover what genes, when damaged, can cause these tumors. This will help us decide how best to treat this very common and often lethal disease. ..
  3. Sleeping Beauty for uncovering cancer genes in mice
    DAVID ANDREW LARGAESPADA; Fiscal Year: 2010
    ..This project will be extended upon and be used in new unbiased screens to define genetic changes that can cause cancer and create useful in vivo models for future studies. ..
  4. Transposon-based somatic mutagenesis/prostate cancer genetics
    DAVID LARGAESPADA; Fiscal Year: 2007
    ..Finally, this approach represents a novel method for the unbiased molecular/genetic analysis of cancer development and could be used widely in the study of important clinical cancer problems. ..
  5. Sleeping Beauty for uncovering cancer genes in mice
    DAVID LARGAESPADA; Fiscal Year: 2007
    ..This project will be extended upon and be used in new unbiased screens to define genetic changes that can cause cancer and create useful in vivo models for future studies. ..
  6. Transposon Mediated Insertional Mutagenesis in Mice
    DAVID LARGAESPADA; Fiscal Year: 2003
    ..abstract_text> ..
  7. COOPERATING GENETIC EVENTS IN MYELOID LEUKEMIA DEVELOPME
    DAVID LARGAESPADA; Fiscal Year: 2002
    ..It is the goal of this proposal to define the overlap between the oncogenic effects of these mutations and discover other proteins involved in this pathway. ..
  8. NF1 GENE IN MYELOID LEUKEMIA AND CYTOKINE SIGNALING
    DAVID LARGAESPADA; Fiscal Year: 2002
    ....
  9. A Genetic Screen for HCC Genes
    Adam J Dupuy; Fiscal Year: 2011
    ..We will discover what genes, when damaged, can cause these tumors. This will help us decide how best to treat this dangerous forms of cancer. ..