J K Lanyi

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi Bacteriorhodopsin
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Annu Rev Physiol 66:665-88. 2004
  2. ncbi X-ray crystallography of bacteriorhodopsin and its photointermediates: insights into the mechanism of proton transport
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Biochemistry (Mosc) 66:1192-6. 2001
  3. pmc Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 45:12003-10. 2006
  4. ncbi Proton transfers in the bacteriorhodopsin photocycle
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Biochim Biophys Acta 1757:1012-8. 2006
  5. ncbi What is the real crystallographic structure of the L photointermediate of bacteriorhodopsin?
    Janos K Lanyi
    Department of Physiology and Biophysics, College of Medicine, University of California, 349 D Medical Science, Irvine, CA 92697 4560, USA
    Biochim Biophys Acta 1658:14-22. 2004
  6. ncbi X-ray diffraction of bacteriorhodopsin photocycle intermediates
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Mol Membr Biol 21:143-50. 2004
  7. ncbi Mechanism of proton transport in bacteriorhodopsin from crystallographic structures of the K, L, M1, M2, and M2' intermediates of the photocycle
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, 346D Medical Science I, Irvine, CA 92697, USA
    J Mol Biol 328:439-50. 2003
  8. ncbi Crystallographic studies of the conformational changes that drive directional transmembrane ion movement in bacteriorhodopsin
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine 92697, USA
    Biochim Biophys Acta 1459:339-45. 2000
  9. ncbi Progress toward an explicit mechanistic model for the light-driven pump, bacteriorhodopsin
    J K Lanyi
    Department of Physiology, University of California, Irvine, CA, USA
    FEBS Lett 464:103-7. 1999
  10. ncbi Understanding structure and function in the light-driven proton pump bacteriorhodopsin
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, California, 92697, USA
    J Struct Biol 124:164-78. 1998

Research Grants

Collaborators

Detail Information

Publications57

  1. ncbi Bacteriorhodopsin
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Annu Rev Physiol 66:665-88. 2004
    ....
  2. ncbi X-ray crystallography of bacteriorhodopsin and its photointermediates: insights into the mechanism of proton transport
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Biochemistry (Mosc) 66:1192-6. 2001
    ..Future structural work will concentrate on describing the details of the individual proton transfer steps during the photocycle...
  3. pmc Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 45:12003-10. 2006
    ....
  4. ncbi Proton transfers in the bacteriorhodopsin photocycle
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Biochim Biophys Acta 1757:1012-8. 2006
    ..The conceptual and technical advantages of this system have allowed close examination of many of these model reactions, some at an atomic level...
  5. ncbi What is the real crystallographic structure of the L photointermediate of bacteriorhodopsin?
    Janos K Lanyi
    Department of Physiology and Biophysics, College of Medicine, University of California, 349 D Medical Science, Irvine, CA 92697 4560, USA
    Biochim Biophys Acta 1658:14-22. 2004
    ..This article compares the models and their implications, the crystallographic statistics and the methods used to derive them, as well as their agreement with non-crystallographic information...
  6. ncbi X-ray diffraction of bacteriorhodopsin photocycle intermediates
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Mol Membr Biol 21:143-50. 2004
    ..Such local-global conformational coupling, with gradual spreading of a local perturbation over the rest of the protein, might be the uniting principle of transporters and receptors...
  7. ncbi Mechanism of proton transport in bacteriorhodopsin from crystallographic structures of the K, L, M1, M2, and M2' intermediates of the photocycle
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, 346D Medical Science I, Irvine, CA 92697, USA
    J Mol Biol 328:439-50. 2003
    ..The changes provide rationales for how relaxation of the distorted retinal causes movements of water and protein atoms that result in vectorial proton transfers to and from the Schiff base...
  8. ncbi Crystallographic studies of the conformational changes that drive directional transmembrane ion movement in bacteriorhodopsin
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine 92697, USA
    Biochim Biophys Acta 1459:339-45. 2000
    ....
  9. ncbi Progress toward an explicit mechanistic model for the light-driven pump, bacteriorhodopsin
    J K Lanyi
    Department of Physiology, University of California, Irvine, CA, USA
    FEBS Lett 464:103-7. 1999
    ....
  10. ncbi Understanding structure and function in the light-driven proton pump bacteriorhodopsin
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, California, 92697, USA
    J Struct Biol 124:164-78. 1998
    ....
  11. ncbi Local-global conformational coupling in a heptahelical membrane protein: transport mechanism from crystal structures of the nine states in the bacteriorhodopsin photocycle
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Biochemistry 43:3-8. 2004
    ..Such local-global conformational coupling between the ligand-binding site and the distant regions of the protein may be the shared mechanism of ion pumps and G-protein related receptors...
  12. pmc Structural changes in the L photointermediate of bacteriorhodopsin
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 365:1379-92. 2007
    ..Their rationales in the transport can be deduced from the way their amplitudes increase in the intermediates that follow L in the reaction cycle, and from the proton transfer reactions with which they are associated...
  13. ncbi Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Science 286:255-61. 1999
    ..The structural changes detected suggest the means for conserving energy at the active site and for ensuring the directionality of proton translocation...
  14. ncbi Coupling photoisomerization of retinal to directional transport in bacteriorhodopsin
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    J Mol Biol 300:1237-55. 2000
    ..This accounts for the lowering of the pK(a) of Asp96, which then reprotonates the Schiff base via a newly formed chain of water molecules that is extending toward the Schiff base...
  15. ncbi Photochemical reaction cycle and proton transfers in Neurospora rhodopsin
    L S Brown
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    J Biol Chem 276:32495-505. 2001
    ..Fourier-transform Raman spectroscopy revealed unexpected differences between NR and bacteriorhodopsin in the configuration of the retinal chromophore, which may contribute to the less effective reprotonation switch of NR...
  16. ncbi Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate
    S Rouhani
    Life Sciences Division, Donner Laboratory, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    J Mol Biol 313:615-28. 2001
    ..On the cytoplasmic side, there is increased hydration near the surface, suggesting how Asp96 might communicate with the bulk during the rise of the O intermediate...
  17. ncbi Coupling of the reisomerization of the retinal, proton uptake, and reprotonation of Asp-96 in the N photointermediate of bacteriorhodopsin
    A K Dioumaev
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 40:11308-17. 2001
    ..The reisomerization of the retinal occurs only after recovery from a long-living protein conformation in which reprotonation of Asp-96 is either entirely blocked or blocked at low pH...
  18. ncbi Crystal structure of sensory rhodopsin II at 2.4 angstroms: insights into color tuning and transducer interaction
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Science 293:1499-503. 2001
    ..We propose that this residue interacts with the adjacent helices of the cognate NpSRII transducer NpHtrII...
  19. ncbi Proton transfer pathways in bacteriorhodopsin at 2.3 angstrom resolution
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Science 280:1934-7. 1998
    ..The nearby Arg82 is the center of a network of numerous hydrogen-bonded residues and an ordered water molecule. This network defines the pathway of the proton from the buried Schiff base to the extracellular surface...
  20. pmc Reconstitution of Gloeobacter violaceus rhodopsin with a light-harvesting carotenoid antenna
    Eleonora S Imasheva
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 48:10948-55. 2009
    ....
  21. ncbi Bacteriorhodopsin
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Curr Opin Struct Biol 11:415-9. 2001
    ....
  22. ncbi Structure of bacteriorhodopsin at 1.55 A resolution
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA
    J Mol Biol 291:899-911. 1999
    ..A bilayer of 18 tightly bound lipid chains forms an annulus around the protein in the crystal. Contacts between the trimers in the membrane plane are mediated almost exclusively by lipids...
  23. ncbi Selectivity of retinal photoisomerization in proteorhodopsin is controlled by aspartic acid 227
    Eleonora S Imasheva
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 43:1648-55. 2004
    ..We conclude that the photoisomerization pathways in proteorhodopsin to 13-cis or 9-cis photoproducts are controlled by the charge state of Asp227...
  24. ncbi Crystallographic structures of the M and N intermediates of bacteriorhodopsin: assembly of a hydrogen-bonded chain of water molecules between Asp-96 and the retinal Schiff base
    Brigitte Schobert
    Department of Physiology and Biophysics, University of California, D345 Medical Science I, Irvine, CA 92697, USA
    J Mol Biol 330:553-70. 2003
    ..This suggests that the hydration of the cytoplasmic region we observe in N' might have occurred spontaneously, beginning at an existing water molecule as nucleus, in the cavities from residue rearrangements in the photocycle...
  25. ncbi Proton transfers in the photochemical reaction cycle of proteorhodopsin
    Andrei K Dioumaev
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 41:5348-58. 2002
    ..The decay of this N-like state is coupled to reisomerization of the retinal to all-trans, and produces a state that is O-like in its C-C stretch bands, but has an absorption maximum apparently close to that of unphotolyzed PR...
  26. pmc Crystallographic structure of xanthorhodopsin, the light-driven proton pump with a dual chromophore
    Hartmut Luecke
    Departments of Molecular Biology and Biochemistry, and Physiology and Biophysics, and Center for Biomembrane Systems, University of California, Irvine, CA 92697
    Proc Natl Acad Sci U S A 105:16561-5. 2008
    ....
  27. pmc Aspartate-histidine interaction in the retinal schiff base counterion of the light-driven proton pump of Exiguobacterium sibiricum
    S P Balashov
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Biochemistry 51:5748-62. 2012
    ....
  28. ncbi Proton transport by proteorhodopsin requires that the retinal Schiff base counterion Asp-97 be anionic
    Andrei K Dioumaev
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 42:6582-7. 2003
    ..Instead, proton transport activity followed the titration curve for Asp-97, with an apparent pK(a) of 7.1, and became zero at the pH where Asp-97 is fully protonated...
  29. pmc Chromophore interaction in xanthorhodopsin--retinal dependence of salinixanthin binding
    Eleonora S Imasheva
    Department of Physiology and Biophysics, University of California, Irvine, CA, USA
    Photochem Photobiol 84:977-84. 2008
    ..This is confirmed by the results for retinol reconstitution, where the Schiff base does not form but the carotenoid exhibits its characteristic spectral change from the binding...
  30. pmc Bacteriorhodopsin photocycle at cryogenic temperatures reveals distributed barriers of conformational substates
    Andrei K Dioumaev
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 104:9621-6. 2007
    ....
  31. pmc pH-dependent transitions in xanthorhodopsin
    Eleonora S Imasheva
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Photochem Photobiol 82:1406-13. 2006
    ..0 is apparently caused by protonation of the counterion and proton acceptor, and that at high pH reflects the pKa of the internal proton donor, Glu94, at the times in the photocycle when this group equilibrates with the bulk...
  32. ncbi Xanthorhodopsin: Proton pump with a carotenoid antenna
    S P Balashov
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Cell Mol Life Sci 64:2323-8. 2007
    ..Antenna addition to a retinal protein has not been observed among the archaea and emerged in bacteria apparently in response to environmental conditions where light-harvesting becomes a limiting factor in retinal protein functioning...
  33. pmc Low-temperature FTIR study of multiple K intermediates in the photocycles of bacteriorhodopsin and xanthorhodopsin
    Andrei K Dioumaev
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA 5R37GM029498
    J Phys Chem B 114:2920-31. 2010
    ..In bacteriorhodopsin, the most likely candidate is water 407. However, unlike bacteriorhodopsin, the crystal structure of xanthorhodopsin lacks homologous weakly bound water molecules...
  34. pmc Electron paramagnetic resonance study of structural changes in the O photointermediate of bacteriorhodopsin
    Deliang Chen
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 366:790-805. 2007
    ....
  35. ncbi Conformational change of the E-F interhelical loop in the M photointermediate of bacteriorhodopsin
    Leonid S Brown
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 317:471-8. 2002
    ..Thus, the motion of the loop in M is more complex than expected from simple tilt of helix F, and may include rotation that unwinds its twist...
  36. ncbi Studies of the bacteriorhodopsin photocycle without the use of light: clues to proton transfer coupled reactions
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, California 92697 4560, USA
    J Mol Microbiol Biotechnol 12:210-7. 2007
    ..This review recounts experiments of this kind, and what they contribute to understanding the transport mechanism of this pump, and perhaps indirectly other ion pumps as well...
  37. pmc Excitation energy-transfer and the relative orientation of retinal and carotenoid in xanthorhodopsin
    Sergei P Balashov
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biophys J 95:2402-14. 2008
    ..The results establish xanthorhodopsin as the simplest biological excited-state donor-acceptor system for collecting light...
  38. pmc Switch from conventional to distributed kinetics in the bacteriorhodopsin photocycle
    Andrei K Dioumaev
    Department of Physiology and Biophysics, University of California, Irvine, California 92697 4560, USA
    Biochemistry 47:11125-33. 2008
    ....
  39. ncbi Structural clues to the mechanism of ion pumping in bacteriorhodopsin
    Hartmut Luecke
    Departments of Biochemistry, Biophysics and Computer Science, University of California, Irvine, California 92697, USA
    Adv Protein Chem 63:111-30. 2003
  40. pmc Infrared monitoring of interlayer water in stacks of purple membranes
    Andrei K Dioumaev
    Department of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA
    Photochem Photobiol 85:598-608. 2009
    ..However, freezing of interbilayer water does block the M to N transition. Unlike the water, the purple membrane lipids do not undergo any IR-detectable phase transition in the 180-280 K range...
  41. pmc Xanthorhodopsin: a bacteriorhodopsin-like proton pump with a carotenoid antenna
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Biochim Biophys Acta 1777:684-8. 2008
    ..This antenna system, simpler than photosynthetic complexes, is well-suited to study features of excited-state energy migration...
  42. pmc Induced chirality of the light-harvesting carotenoid salinixanthin and its interaction with the retinal of xanthorhodopsin
    Sergei P Balashov
    Department of Physiology and Biophysics, University of California, D340 Medical Science I, Irvine, California 92697, USA
    Biochemistry 45:10998-1004. 2006
    ..The observed spectral features suggest that binding of salinixanthin in xanthorhodopsin involves the cyclohexenone ring of the carotenoid and its conformational heterogeneity is restricted...
  43. pmc Two bathointermediates of the bacteriorhodopsin photocycle, from time-resolved nanosecond spectra in the visible
    Andrei K Dioumaev
    Department of Physiology and Biophysics, University of California, Irvine, Irvine, California 92697, USA
    J Phys Chem B 113:16643-53. 2009
    ..The consequences of multiple K-like states for interpreting the spectral evolution in the early stages of the photocycle are discussed...
  44. pmc Reconstitution of gloeobacter rhodopsin with echinenone: role of the 4-keto group
    Sergei P Balashov
    Department of Physiology and Biophysics, University of California, Irvine, California 92697 4560, United States
    Biochemistry 49:9792-9. 2010
    ..We suggest that the 4-keto group in salinixanthin and echinenone allows for the twisted conformation of the ring around the C6-C7 bond and probably is engaged in an interaction that locks the carotenoid in the binding site...
  45. pmc Xanthorhodopsin: a proton pump with a light-harvesting carotenoid antenna
    Sergei P Balashov
    University of California, Irvine, CA 92697, USA
    Science 309:2061-4. 2005
    ..The antenna carotenoid extends the wavelength range of the collection of light for uphill transmembrane proton transport...
  46. ncbi Formation of a long-lived photoproduct with a deprotonated Schiff base in proteorhodopsin, and its enhancement by mutation of Asp227
    Eleonora S Imasheva
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 44:10828-38. 2005
    ..The transformation to the long-lived 362 nm photoproduct is apparently a side reaction of the photocycle, a response to high pH, caused by alteration of the normal reprotonation and reisomerization pathway of the Schiff base...
  47. ncbi Proton pumps: mechanism of action and applications
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697-4560, USA
    Trends Biotechnol 19:140-4. 2001
    ..Other possible applications of such biochemical devices include targeted drug delivery and biocatalytic reactors. All these devices might prove superior to their inorganic alternatives...
  48. ncbi The primary structure of a halorhodopsin from Natronobacterium pharaonis. Structural, functional and evolutionary implications for bacterial rhodopsins and halorhodopsins
    J K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine 92717
    J Biol Chem 265:1253-60. 1990
    ..Comparison of the bacteriorhodopsin sequence with those of the two halorhodopsins, on the other hand, identifies features involved in their specific (proton and chloride ion) transport functions...
  49. pmc Structural changes in the N and N' states of the bacteriorhodopsin photocycle
    Deliang Chen
    Department of Physiology and Biophysics, University of California, Irvine, California, USA
    Biophys J 96:2779-88. 2009
    ..The structural changes reverse their directions and decay in the N' state...
  50. pmc The photochemical reaction cycle of proteorhodopsin at low pH
    Melinda Lakatos
    Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Temesvari krt 62, H 6701 Hungary
    Biophys J 84:3252-6. 2003
    ....
  51. ncbi Changes in hydrogen bonding and environment of tryptophan residues on helix F of bacteriorhodopsin during the photocycle: a time-resolved ultraviolet resonance Raman study
    Shinji Hashimoto
    Faculty of Science and Engineering, Tokyo University of Science, Yamaguchi, Daigaku Dori, Onoda 756 0884, Japan
    Biochemistry 41:6495-503. 2002
    ..Structural reorganization of the protein after the tilt of helix F may be important for efficient reprotonation of the Schiff base...
  52. pmc A priori resolution of the intermediate spectra in the bacteriorhodopsin photocycle: the time evolution of the L spectrum revealed
    László Zimányi
    J Phys Chem A 110:2318-21. 2006
    ..We suggest that these subtle differences in the shapes of the spectra reflect torsional and/or environmental differences in the retinyl chromophore...
  53. pmc Characterization of the photochemical reaction cycle of proteorhodopsin
    György Váró
    Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, H 6701, Hungary
    Biophys J 84:1202-7. 2003
    ..These states give the major contributions to the proton translocation across the membrane. The energetic scheme of the photocycle was calculated from the temperature-dependence of the absorption kinetic signals...
  54. pmc Functions of carotenoids in xanthorhodopsin and archaerhodopsin, from action spectra of photoinhibition of cell respiration
    Vladimir A Boichenko
    Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia
    Biochim Biophys Acta 1757:1649-56. 2006
    ..This emphasizes the uniqueness of the salinixanthin-retinal interaction in xanthorhodopsin, and indicates that bacterioruberin in Halorubrum species has a structural or photoprotective rather than energetic role...
  55. pmc Characterization of the azide-dependent bacteriorhodopsin-like photocycle of salinarum halorhodopsin
    Melinda Lakatos
    Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, H 6701, Hungary
    Biophys J 82:1687-95. 2002
    ..The differences between the azide-dependent photocycle of salinarum halorhodopsin and pharaonis halorhodopsin are discussed...
  56. pmc The nitrate transporting photochemical reaction cycle of the pharaonis halorhodopsin
    Zoltan Balint
    Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, H 6701, Hungary
    Biophys J 86:1655-63. 2004
    ..The release occurs during the N-to-O transition, whereas the rebinding happens in several seconds, well after the other steps of the photocycle are over...
  57. pmc Proton binding within a membrane protein by a protonated water cluster
    Florian Garczarek
    Lehrstuhl fur Biophysik, Ruhr Universitat Bochum, D 44780 Bochum, Germany
    Proc Natl Acad Sci U S A 102:3633-8. 2005
    ..Thus, the release mechanism switches between the wild-type and mutated proteins from a delocalized to a localized proton-binding site...

Research Grants1

  1. LIGHT-DRIVEN ION TRANSPORT IN BACTERIAL RHODOPSINS
    Janos Lanyi; Fiscal Year: 2007
    ..The goal of this proposal is to establish, finally, a full and explicit model for the molecular mechanism of this kind of ion pump. ..