Robert Landick

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Complex physiology and compound stress responses during fermentation of alkali-pretreated corn stover hydrolysate by an Escherichia coli ethanologen
    Michael S Schwalbach
    Great Lakes Bioenergy Research Center, Madison, Wisconsin, USA
    Appl Environ Microbiol 78:3442-57. 2012
  2. pmc The bridge helix coordinates movements of modules in RNA polymerase
    Pyae P Hein
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    BMC Biol 8:141. 2010
  3. pmc Real-time footprinting of DNA in the first kinetically significant intermediate in open complex formation by Escherichia coli RNA polymerase
    Caroline A Davis
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 104:7833-8. 2007
  4. ncbi request reprint A hydrophobic patch on the flap-tip helix of E.coli RNA polymerase mediates sigma(70) region 4 function
    Kati Geszvain
    Department of Genetics, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 343:569-87. 2004
  5. ncbi request reprint A new class of bacterial RNA polymerase inhibitor affects nucleotide addition
    Irina Artsimovitch
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Science 302:650-4. 2003
  6. pmc The RPB2 flap loop of human RNA polymerase II is dispensable for transcription initiation and elongation
    Murali Palangat
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Mol Cell Biol 31:3312-25. 2011
  7. pmc Role of the RNA polymerase trigger loop in catalysis and pausing
    Jinwei Zhang
    Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA
    Nat Struct Mol Biol 17:99-104. 2010
  8. pmc Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators
    Rachel Anne Mooney
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    J Mol Biol 391:341-58. 2009
  9. ncbi request reprint A long time in the making--the Nobel Prize for RNA polymerase
    Robert Landick
    Department of Bacteriology, 420 Henry Mall, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell 127:1087-90. 2006
  10. ncbi request reprint The role of the lid element in transcription by E. coli RNA polymerase
    Innokenti Toulokhonov
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 361:644-58. 2006

Collaborators

Detail Information

Publications26

  1. pmc Complex physiology and compound stress responses during fermentation of alkali-pretreated corn stover hydrolysate by an Escherichia coli ethanologen
    Michael S Schwalbach
    Great Lakes Bioenergy Research Center, Madison, Wisconsin, USA
    Appl Environ Microbiol 78:3442-57. 2012
    ....
  2. pmc The bridge helix coordinates movements of modules in RNA polymerase
    Pyae P Hein
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    BMC Biol 8:141. 2010
    ....
  3. pmc Real-time footprinting of DNA in the first kinetically significant intermediate in open complex formation by Escherichia coli RNA polymerase
    Caroline A Davis
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 104:7833-8. 2007
    ....
  4. ncbi request reprint A hydrophobic patch on the flap-tip helix of E.coli RNA polymerase mediates sigma(70) region 4 function
    Kati Geszvain
    Department of Genetics, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 343:569-87. 2004
    ..Our studies failed to detect any role for sigma region 1.1 in establishing or maintaining the flap-sigma region 4 interaction, consistent with recent reports placing sigma region 1.1 in the downstream DNA channel...
  5. ncbi request reprint A new class of bacterial RNA polymerase inhibitor affects nucleotide addition
    Irina Artsimovitch
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Science 302:650-4. 2003
    ..We propose that CBR703 compounds inhibit nucleotide addition allosterically by hindering movements of active site structures that are linked to the CBR703 binding site through a bridge helix...
  6. pmc The RPB2 flap loop of human RNA polymerase II is dispensable for transcription initiation and elongation
    Murali Palangat
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Mol Cell Biol 31:3312-25. 2011
    ..A modest effect on transcript elongation and pausing was suppressed by TFIIF. Although similar to the flap tip of bacterial RNAP, the RNAPII flap loop is not equivalently essential...
  7. pmc Role of the RNA polymerase trigger loop in catalysis and pausing
    Jinwei Zhang
    Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA
    Nat Struct Mol Biol 17:99-104. 2010
    ..The similar effects of TL/TH substitutions on pausing and nucleotide addition provide additional support for the view that TH formation is rate-limiting for escape from nonbacktracked pauses...
  8. pmc Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators
    Rachel Anne Mooney
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    J Mol Biol 391:341-58. 2009
    ....
  9. ncbi request reprint A long time in the making--the Nobel Prize for RNA polymerase
    Robert Landick
    Department of Bacteriology, 420 Henry Mall, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell 127:1087-90. 2006
    ..The prize caps a decades-long quest to unlock one of the central mysteries of molecular biology-how RNA transcripts are assembled...
  10. ncbi request reprint The role of the lid element in transcription by E. coli RNA polymerase
    Innokenti Toulokhonov
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 361:644-58. 2006
    ..Importantly, Deltalid RNAP behaved differently from wild-type RNAP when transcribing single-stranded DNA templates where it synthesized long, persistent RNA:DNA hybrids, in contrast to efficient transcriptional arrest by wild-type RNAP...
  11. ncbi request reprint NTP-entry routes in multi-subunit RNA polymerases
    Robert Landick
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Trends Biochem Sci 30:651-4. 2005
    ..Recently, an alternative view has been advocated; namely, NTPs enter the active site pre-bound to the DNA template from the downstream DNA portion of the main channel of the enzyme...
  12. pmc Tethering sigma70 to RNA polymerase reveals high in vivo activity of sigma factors and sigma70-dependent pausing at promoter-distal locations
    Rachel Anne Mooney
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genes Dev 17:2839-51. 2003
    ..coli (i.e., its thermodynamic activity) is close to its bulk concentration. At this level, sigma70 would be a bona fide elongation factor able to direct transcriptional pausing even after its release from RNAP during promoter escape...
  13. ncbi request reprint Sigma and RNA polymerase: an on-again, off-again relationship?
    Rachel Anne Mooney
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Mol Cell 20:335-45. 2005
    ....
  14. pmc Rho directs widespread termination of intragenic and stable RNA transcription
    Jason M Peters
    Department of Biochemistry, University of Wisconsin, Madison, WI 5370, USA
    Proc Natl Acad Sci U S A 106:15406-11. 2009
    ..These Rho-terminated antisense transcripts point to a role of noncoding transcription in E. coli gene regulation that may resemble the ubiquitous noncoding transcription recently found to play myriad roles in eukaryotic gene regulation...
  15. ncbi request reprint Downstream DNA selectively affects a paused conformation of human RNA polymerase II
    Murali Palangat
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 341:429-42. 2004
    ..These findings provide a new framework for understanding downstream DNA effects on RNAP...
  16. pmc Regulator trafficking on bacterial transcription units in vivo
    Rachel A Mooney
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Cell 33:97-108. 2009
    ..However, inhibition of rho did not increase RNAP levels within genes downstream from the RNAP peaks, suggesting the peaks are caused by a mechanism other than rho-dependent attenuation...
  17. pmc The NusA N-terminal domain is necessary and sufficient for enhancement of transcriptional pausing via interaction with the RNA exit channel of RNA polymerase
    Kook Sun Ha
    Department of Biomolecular Chemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 401:708-25. 2010
    ..Finally, we report evidence that the NusA NTD recognizes duplex RNA in the RNA exit channel...
  18. ncbi request reprint Direct versus limited-step reconstitution reveals key features of an RNA hairpin-stabilized paused transcription complex
    Scotty Kyzer
    Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 282:19020-8. 2007
    ..Reconstitution of the his paused transcription complex provides a valuable tool for future studies of protein-nucleic interactions involved in transcriptional pausing...
  19. pmc A negative elongation factor for human RNA polymerase II inhibits the anti-arrest transcript-cleavage factor TFIIS
    Murali Palangat
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 102:15036-41. 2005
    ..Because TFIIS promotes escape from promoter-proximal pauses by stimulating cleavage of back-tracked nascent RNA, TFIIS inhibition may help DSIF/NELF negatively regulate productive transcription...
  20. ncbi request reprint Active-site dynamics in RNA polymerases
    Robert Landick
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell 116:351-3. 2004
    ....
  21. ncbi request reprint A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing
    Innokenti Toulokhonov
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Cell 27:406-19. 2007
    ..This trigger-loop-centric model can explain many properties of transcriptional pausing...
  22. ncbi request reprint The flap domain is required for pause RNA hairpin inhibition of catalysis by RNA polymerase and can modulate intrinsic termination
    Innokenti Toulokhonov
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Mol Cell 12:1125-36. 2003
    ..In contrast, the flap tip is not required for intrinsic termination, but can modulate it at suboptimal termination signals...
  23. ncbi request reprint Inhibition of RNA polymerase by streptolydigin: no cycling allowed
    Scotty Kyzer
    Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Cell 122:494-6. 2005
    ....
  24. ncbi request reprint The transcriptional regulator RfaH stimulates RNA chain synthesis after recruitment to elongation complexes by the exposed nontemplate DNA strand
    Irina Artsimovitch
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    Cell 109:193-203. 2002
    ..This activity predicts a cumulative antitermination model for RfaH's regulation of ops-containing operons in vivo...
  25. pmc Organization and evolution of the biological response to singlet oxygen stress
    Yann S Dufour
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 383:713-30. 2008
    ..Our analysis also predicts that the response to (1)O(2) evolved within the time frame of the accumulation of atmospheric molecular oxygen on this planet...