J R Lakowicz

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. ncbi request reprint Emerging applications of fluorescence spectroscopy to cellular imaging: lifetime imaging, metal-ligand probes, multi-photon excitation and light quenching
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore
    Scanning Microsc Suppl 10:213-24. 1996
  2. pmc Time-resolved fluorescence spectroscopy and imaging of DNA labeled with DAPI and Hoechst 33342 using three-photon excitation
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA
    Biophys J 72:567-78. 1997
  3. ncbi request reprint On the possibility of long-wavelength long-lifetime high-quantum-yield luminophores
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 288:62-75. 2001
  4. ncbi request reprint Two-color two-photon excitation of fluorescence
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA
    Photochem Photobiol 64:632-5. 1996
  5. ncbi request reprint Frequency-domain fluorescence microscopy with the LED as a light source
    P Herman
    University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Microsc 203:176-81. 2001
  6. ncbi request reprint Fluorescence of reduced nicotinamides using one- and two-photon excitation
    B Kierdaszuk
    Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201, USA
    Biophys Chem 62:1-13. 1996
  7. ncbi request reprint Novel fluorescence sensing methods for high throughput screening
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Biomol Screen 5:123-32. 2000
  8. ncbi request reprint On the possibility of evanescent wave excitation distal from a solid-liquid interface using light quenching
    J R Lakowicz
    Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201 1503, USA
    Photochem Photobiol 64:636-41. 1996
  9. ncbi request reprint Intrinsic fluorescence from DNA can be enhanced by metallic particles
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Biochem Biophys Res Commun 286:875-9. 2001
  10. doi request reprint Multiphoton excitation of lanthanides
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Deptartment of Biochemistry and Molecular Biology, School of Medicine, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Chemphyschem 2:247-52. 2001

Collaborators

  • K Ray
  • Y Fu
  • J Zhang
  • B Shen
  • M Schrader
  • S W Hell
  • P Herman
  • H J Lin
  • B P Maliwal
  • N DiCesare
  • J Digel
  • S Murata
  • O O Abugo
  • Z Gryczynski
  • J D Dattelbaum
  • B Kierdaszuk
  • T Kobayashi
  • J S Kang
  • O Abugo
  • J H Collins
  • J Sipior
  • H Malak
  • P Callis
  • I Gryczynski
  • G M Carter
  • S Bambot
  • M Romauld
  • G Rao

Detail Information

Publications20

  1. ncbi request reprint Emerging applications of fluorescence spectroscopy to cellular imaging: lifetime imaging, metal-ligand probes, multi-photon excitation and light quenching
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore
    Scanning Microsc Suppl 10:213-24. 1996
    ..This use of non-absorbed light to modify emission can have wide reaching applications in cellular imaging...
  2. pmc Time-resolved fluorescence spectroscopy and imaging of DNA labeled with DAPI and Hoechst 33342 using three-photon excitation
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA
    Biophys J 72:567-78. 1997
    ..Three-photon excitation of DAPI-stained chromosomes at 970 nm was demonstrated by the power dependence in the fluorescence microscope...
  3. ncbi request reprint On the possibility of long-wavelength long-lifetime high-quantum-yield luminophores
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 288:62-75. 2001
    ..These results suggest the synthesis of covalently linked donor-acceptor pairs with the desirable spectral properties of long wavelength emission, high quantum yield, and moderately long lifetimes for gated detection...
  4. ncbi request reprint Two-color two-photon excitation of fluorescence
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA
    Photochem Photobiol 64:632-5. 1996
    ..The amplitude of the signal depended on the polarization of each beam. 2C2P excitation can have applications in fluorescence microscopy and elsewhere when spatially localized excitation is desirable...
  5. ncbi request reprint Frequency-domain fluorescence microscopy with the LED as a light source
    P Herman
    University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Microsc 203:176-81. 2001
    ..The ability to select an excitation source for a given fluorophore and low price make such an excitation source even more practical...
  6. ncbi request reprint Fluorescence of reduced nicotinamides using one- and two-photon excitation
    B Kierdaszuk
    Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201, USA
    Biophys Chem 62:1-13. 1996
    ....
  7. ncbi request reprint Novel fluorescence sensing methods for high throughput screening
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Biomol Screen 5:123-32. 2000
    ..Sensing with a reference film provides the opportunity to internally reference HTS measurements without the need for additions to the sample. This approach can provide standardization for assays performed at different times...
  8. ncbi request reprint On the possibility of evanescent wave excitation distal from a solid-liquid interface using light quenching
    J R Lakowicz
    Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201 1503, USA
    Photochem Photobiol 64:636-41. 1996
    ....
  9. ncbi request reprint Intrinsic fluorescence from DNA can be enhanced by metallic particles
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Biochem Biophys Res Commun 286:875-9. 2001
    ....
  10. doi request reprint Multiphoton excitation of lanthanides
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Deptartment of Biochemistry and Molecular Biology, School of Medicine, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Chemphyschem 2:247-52. 2001
    ..This suggests the use of lanthanides as calcium analogues in multiphoton microscopy. The spectrum shows the normalized two-photon excitation emission spectra (╬╗ex = 796 nm) of Eu(3+) with a resolution of about 8 nm...
  11. ncbi request reprint Fluorescence properties of albumin blue 633 and 670 in plasma and whole blood
    O O Abugo
    University of Maryland at Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Biomed Opt 6:359-65. 2001
    ....
  12. ncbi request reprint Fluorescence lifetime characterization of novel low-pH probes
    H J Lin
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 294:118-25. 2001
    ..These results indicate that LysoSensor DND-160 is a promising probe for lifetime imaging to determine lysosomal pH...
  13. ncbi request reprint Evaluation of two synthetic glucose probes for fluorescence-lifetime-based sensing
    N DiCesare
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 W. Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 294:154-60. 2001
    ..Combining these results and the use of a simple UV-LED as excitation source, the results show an interesting potential of these two compounds in the development of lifetime base devices using synthetic probes for glucose...
  14. ncbi request reprint Calcium- and magnesium-dependent interactions between the C-terminus of troponin I and the N-terminal, regulatory domain of troponin C
    J Digel
    Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore 21201, USA
    Arch Biochem Biophys 387:243-9. 2001
    ..Our results provide the first indica- tion that the C-terminus of TnI may play an important role in the regulation of vertebrate striated muscle through Ca2+-dependent interactions with the regula- tory domain of TnC...
  15. ncbi request reprint Long-wavelength long-lifetime luminophores
    B P Maliwal
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 21201, USA
    Anal Chem 73:4277-85. 2001
    ..Luminophores with long-wavelength emission and long lifetimes can have numerous applications in biophysics, clinical diagnostics, DNA analysis, and drug discovery...
  16. ncbi request reprint Texture analysis of fluorescence lifetime images of AT- and GC-rich regions in nuclei
    S Murata
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, School of Medicine, Baltimore, Maryland 21201, USA
    J Histochem Cytochem 49:1443-51. 2001
    ..Texture analysis of fluorescence intensity and lifetime images was used to quantitatively study the spatial change of condensation and separation of AT- and GC-rich DNA during the cell cycle...
  17. ncbi request reprint A lifetime-based optical CO2 gas sensor with blue or red excitation and stokes or anti-stokes detection
    J Sipior
    Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201 1503, USA
    Anal Biochem 227:309-18. 1995
    ..The use of fluorescence resonance energy transfer, along with the simple entrainment procedure, allows facile change of the CO2 response range through change of the acceptor dye and the use of laser diode excitation sources...
  18. ncbi request reprint Optical determination of glutamine using a genetically engineered protein
    J D Dattelbaum
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland, School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 291:89-95. 2001
    ..The engineered GlnBP is a first step toward the development of a nonenzymatic biosensor capable of determining glutamine concentrations in cell cultures...
  19. ncbi request reprint Plasmon-controlled fluorescence towards high-sensitivity optical sensing
    K Ray
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 W Lombard St, Baltimore, MD, 21201, USA
    Adv Biochem Eng Biotechnol 116:29-72. 2009
    ..Recent research combining plasmonics and fluorescence suggest that PCF could lead to new classes of experimental procedures, novel probes, bioassays, and devices...
  20. ncbi request reprint Radiative decay engineering: biophysical and biomedical applications
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 W. Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 298:1-24. 2001
    ..As one example we predict that nearby metal surfaces can be used to increase the low intrinsic quantum yields of nucleic acids and make unlabeled DNA detectable using its intrinsic metal-enhanced fluorescence...