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| J R LakowiczSummaryAffiliation: University of Maryland Country: USA Publications
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Publications
Radiative decay engineering 5: metal-enhanced fluorescence and plasmon emissionJoseph R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 337:171-94. 2005..The RP model provides a rational approach for designing fluorophore-metal configurations with the desired emissive properties and a basis for nanophotonic fluorophore technology...- Ultrabright fluorescein-labeled antibodies near silver metallic surfacesJoseph R Lakowicz
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry, 725 West Lombard Street, Baltimore, MD 21201, USA
Biopolymers 74:467-75. 2004..These results suggest the use of labeled antibodies conjugated to silver particles as ultrabright probes for imaging or analytical applications... - Radiative decay engineering 3. Surface plasmon-coupled directional emissionJoseph R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 324:153-69. 2004..Examples showing how simple optical configurations can be used in diagnostics, sensing, or biotechnology applications are presented. Surface plasmon-coupled emission is likely to find widespread applications throughout the biosciences... - Silver particles enhance emission of fluorescent DNA oligomersJoseph R Lakowicz
University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
Biotechniques 34:62-6, 68. 2003..Proximity to these silver particles also increases the photostability of Cy3- and Cy5-labeled oligomers. These results suggest the use of DNA array substrates with silver particles for increased sensitivity in genetic analysis... - Enhanced fluorescence of Cy5-labeled oligonucleotides near silver island films: a distance effect study using single molecule spectroscopyYi Fu
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, Baltimore, Maryland 21201 USA
J Phys Chem B 110:22557-62. 2006..These studies illustrate the exclusive information that is otherwise hidden in ensemble measurements... - Plasmonic technology: novel approach to ultrasensitive immunoassaysJoseph R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Clin Chem 51:1914-22. 2005.... - Advances in surface-enhanced fluorescenceJoseph R Lakowicz
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Fluoresc 14:425-41. 2004..We discuss a number of methods for various shaped silver particle deposition on surfaces... 
Novel fluorescence sensing methods for high throughput screeningJ R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
J Biomol Screen 5:123-32. 2000..Sensing with a reference film provides the opportunity to internally reference HTS measurements without the need for additions to the sample. This approach can provide standardization for assays performed at different times...- Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotidesJoanna Malicka
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Anal Biochem 315:57-66. 2003..These results suggest that maximum fluorescence enhancements occur about 90A from the silver surface, a distance readily obtained by one or two layers of proteins... - Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensingKadir Aslan
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Am Chem Soc 127:12115-21. 2005.... - Time-resolved fluorescence spectroscopy and imaging of DNA labeled with DAPI and Hoechst 33342 using three-photon excitationJ R Lakowicz
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA
Biophys J 72:567-78. 1997..Three-photon excitation of DAPI-stained chromosomes at 970 nm was demonstrated by the power dependence in the fluorescence microscope... - Fluorescence enhancements on silver colloid coated surfacesJoanna Lukomska
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Fluoresc 14:417-23. 2004..Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence... - Photodeposition of silver can result in metal-enhanced fluorescenceChris D Geddes
University Maryland Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Appl Spectrosc 57:526-31. 2003..These results demonstrate the possibility of photolithographic preparation of surfaces for enhanced fluorescence in microfluidics, medical diagnostics, and other applications... - On the possibility of long-wavelength long-lifetime high-quantum-yield luminophoresJ R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Anal Biochem 288:62-75. 2001..These results suggest the synthesis of covalently linked donor-acceptor pairs with the desirable spectral properties of long wavelength emission, high quantum yield, and moderately long lifetimes for gated detection... - Saccharide sensing using gold and silver nanoparticles--a reviewKadir Aslan
Center for Fluorescence Spectroscopy, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Fluoresc 14:391-400. 2004..Lifetime measurements were used to distinguish the contribution from the surface-enhanced fluorescence. TEM was employed to assess the aggregation of nanoparticles... - Enhanced emission of highly labeled DNA oligomers near silver metallic surfacesJoanna Malicka
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Anal Chem 75:4408-14. 2003..The increased intensity did not result in increased photobleaching. These results suggest the use of substrates coated with silver particles for increased sensitivity on DNA arrays or for DNA analysis... - Plasmonic enhancement of single-molecule fluorescence near a silver nanoparticleYi Fu
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 W Lombard Street, Baltimore, MD 21201, USA
J Fluoresc 17:811-6. 2007..These results demonstrate that the use of fluorophore-metal interactions can increase the brightness and photostability of fluorophores for single molecule detection... - Metallic colloid wavelength-ratiometric scattering sensorsDavid Roll
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Anal Chem 75:3440-5. 2003..This approach can be used in a wide variety of assay formats, including those commonly used with fluorescence detection... - Fluorescence images of DNA-bound YOYO between coupled silver particlesJian Zhang
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Langmuir 23:11734-9. 2007..The shortened lifetime of YOYO molecules between the coupled metal particles indicated that the fluorescence was enhanced via a near-field interaction mechanism between the fluorophore and the metal nanoparticle... - A model for DNA detection by metal-enhanced fluorescence from immobilized silver nanoparticles on solid substrateJian Zhang
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Phys Chem B 110:2387-92. 2006..This result reveals that increasing the particle layer contributes to fluorescence enhancement. This novel method was used to detect DNA hybridization through both absorbance and emission spectral changes... - Two-color two-photon excitation of fluorescenceJ R Lakowicz
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA
Photochem Photobiol 64:632-5. 1996..The amplitude of the signal depended on the polarization of each beam. 2C2P excitation can have applications in fluorescence microscopy and elsewhere when spatially localized excitation is desirable... - Intrinsic fluorescence from DNA can be enhanced by metallic particlesJ R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Biochem Biophys Res Commun 286:875-9. 2001.... - Radiative decay engineering: biophysical and biomedical applicationsJ R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 W. Lombard Street, Baltimore, Maryland 21201, USA
Anal Biochem 298:1-24. 2001..As one example we predict that nearby metal surfaces can be used to increase the low intrinsic quantum yields of nucleic acids and make unlabeled DNA detectable using its intrinsic metal-enhanced fluorescence... - A lifetime-based optical CO2 gas sensor with blue or red excitation and stokes or anti-stokes detectionJ Sipior
Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201 1503, USA
Anal Biochem 227:309-18. 1995..The use of fluorescence resonance energy transfer, along with the simple entrainment procedure, allows facile change of the CO2 response range through change of the acceptor dye and the use of laser diode excitation sources... - Enhanced fluorescence of proteins and label-free bioassays using aluminum nanostructuresKrishanu Ray
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, Maryland 21201, USA
Anal Chem 81:6049-54. 2009..Binding of streptavidin to the biotinylated aluminum surface was demonstrated in the presence of 100 microg/mL bovine serum albumin in the sample solution by measurements of tryptophan intensity and lifetime changes... - Oligonucleotide-displaced organic monolayer-protected silver nanoparticles and enhanced luminescence of their salted aggregatesJian Zhang
Department of Biochemistry, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 330:81-6. 2004..This result suggests the possible application of surface-enhanced fluorescence from metallic nanoparticle aggregation for DNA detection... - Fluorescence spectral properties of cyanine dye-labeled DNA oligomers on surfaces coated with silver particlesJoanna Malicka
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 317:136-46. 2003..These results suggest the use of substrates containing silver particles for increased sensitivity of DNA detection on DNA arrays... - Metal-enhanced emission from indocyanine green: a new approach to in vivo imagingJoanna Malicka
University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, 725 W. Lombard Street, Baltimore, Maryland 21201-1503, USA
J Biomed Opt 8:472-8. 2003..Since the rate of photobleaching is not increased, our results suggest that ICG-silver particle complexes can yield at least 20-fold more photons per ICG molecule for improved medical imaging... - Multi-wavelength immunoassays using surface plasmon-coupled emissionEvgenia Matveeva
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
Biochem Biophys Res Commun 313:721-6. 2004..This method of SPCE immunoassays can be readily extended to 4 or more wavelengths... - Single-molecule and FRET fluorescence correlation spectroscopy analyses of phage DNA packaging: colocalization of packaged phage T4 DNA ends within the capsidKrishanu Ray
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD 21201, USA
J Mol Biol 395:1102-13. 2010..The analysis suggests that a DNA loop, rather than a DNA end, is translocated by the packaging motor to fill the procapsid... - Fluorescence of reduced nicotinamides using one- and two-photon excitationB Kierdaszuk
Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201, USA
Biophys Chem 62:1-13. 1996.... - On the possibility of evanescent wave excitation distal from a solid-liquid interface using light quenchingJ R Lakowicz
Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201 1503, USA
Photochem Photobiol 64:636-41. 1996.... - Long-wavelength long-lifetime luminophoresB P Maliwal
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 21201, USA
Anal Chem 73:4277-85. 2001..Luminophores with long-wavelength emission and long lifetimes can have numerous applications in biophysics, clinical diagnostics, DNA analysis, and drug discovery... - Roughened silver electrodes for use in metal-enhanced fluorescenceChris D Geddes
Center for Fluorescence Spectroscopy, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, MD 21201, USA
Spectrochim Acta A Mol Biomol Spectrosc 60:1977-83. 2004..To the best of our knowledge this is the first report of roughened silver electrodes for metal-enhanced fluorescence... - Radiative decay engineering. 2. Effects of Silver Island films on fluorescence intensity, lifetimes, and resonance energy transferJoseph R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Anal Biochem 301:261-77. 2002..These results demonstrate that metallic particles can be used to modify the emission from intrinsic and extrinsic fluorophores in biochemical systems... - Plastic versus glass support for an immunoassay on metal-coated surfaces in optically dense samples utilizing directional surface plasmon-coupled emissionEvgenia G Matveeva
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Fluoresc 15:865-71. 2005..We believe that plastic substrates can be readily used in any SPCE assay, with only marginally lower total signal compared to high-quality glass slides... - Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albuminJoanna Malicka
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, MD 21201, USA
Biochem Biophys Res Commun 294:886-92. 2002..6 to 63 A. These results suggest the use of silver island films for detecting long range proximity between biomolecules and for biotechnology applications based on RET... - Metal-enhanced fluorescence immunoassays using total internal reflection and silver island-coated surfacesEvgenia Matveeva
Center for Fluorescence Spectroscopy, Department of Biochemistry Molecular Biology, University of Maryland at Baltimore Medical School, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 334:303-11. 2004..The limited evanescent wave excitation volume makes it possible to minimize the background signal and use the immunoassay with no need for any washing steps... - Fast and slow deposition of silver nanorods on planar surfaces: application to metal-enhanced fluorescenceKadir Aslan
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, USA
J Phys Chem B 109:3157-62. 2005..Subsequently, these new silver nanorod preparation procedures are likely to find a common place in MEF, as they are a quicker and much cheaper alternative as compared to surfaces fabricated by traditional nanolithographic techniques... - Surface-plasmon-coupled emission: new technology for studying molecular processesZygmunt Gryczynski
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, Baltimore, Maryland 21201, USA
Methods Cell Biol 75:73-104. 2004 - Ophthalmic glucose monitoring using disposable contact lenses--a reviewRamachandram Badugu
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Fluoresc 14:617-33. 2004.... - Metal-enhanced chemiluminescenceMustafa H Chowdhury
Center for Fluorescence Spectroscopy, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, MD 21201, USA
J Fluoresc 16:295-9. 2006..This finding is of significance towards our understanding of fluorophore-metal interactions, a relatively new near-field fluorescence concept, recently named metal-enhanced fluorescence and also radiative decay engineering... - Metal-enhanced fluorescence solution-based sensing platformKadir Asian
Institute of Fluorescence, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland, USA
J Fluoresc 14:677-9. 2004..In this short, note, we show how SiO2-coated silver colloids, indeed provide for a solution based enhanced fluorescence sensing platform with a 3-5 fold enhancement typically observed... - Fluorescent avidin-bound silver particle: a strategy for single target molecule detection on a cell membraneJian Zhang
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Anal Chem 81:883-9. 2009..On the basis of this curve, we expect to develop an approach that can be used to quantify the amount of target molecules on the cell surfaces using the cell intensity and lifetime images at the single cell level... - Fluorescence lifetime characterization of novel low-pH probesH J Lin
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Anal Biochem 294:118-25. 2001..These results indicate that LysoSensor DND-160 is a promising probe for lifetime imaging to determine lysosomal pH... - Frequency-domain fluorescence microscopy with the LED as a light sourceP Herman
University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Microsc 203:176-81. 2001..The ability to select an excitation source for a given fluorophore and low price make such an excitation source even more practical... - Emerging applications of fluorescence spectroscopy to cellular imaging: lifetime imaging, metal-ligand probes, multi-photon excitation and light quenchingJ R Lakowicz
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore
Scanning Microsc Suppl 10:213-24. 1996..This use of non-absorbed light to modify emission can have wide reaching applications in cellular imaging... - Single cell fluorescence imaging using metal plasmon-coupled probeJian Zhang
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Bioconjug Chem 18:800-5. 2007..PCPs were conjugated on HEK 293 A cells. Imaging results demonstrate that cells labeled by PCPs were 20-fold brighter than those by free labeled con A... - Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particlesJoanna Malicka
University of Maryland Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 W Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 315:160-9. 2003..These results suggest the use of metal-enhanced RET in PCR, hybridization, and other DNA assays, and the possibility of controlling energy transfer by the distance between silver surfaces... - Plasmon light scattering in biology and medicine: new sensing approaches, visions and perspectivesKadir Aslan
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD 21201, USA
Curr Opin Chem Biol 9:538-44. 2005.... - Plasmon-enhanced fluorescence from single fluorophores end-linked to gold nanorodsYi Fu
University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Am Chem Soc 132:5540-1. 2010..The bioconjugated nanoantenna structure implicates an approach that is more convenient and amenable to integration for photonic, optoelectronic, and biotechnological applications... - Fluorescence properties of albumin blue 633 and 670 in plasma and whole bloodO O Abugo
University of Maryland at Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Biomed Opt 6:359-65. 2001.... - Evaluation of two synthetic glucose probes for fluorescence-lifetime-based sensingN DiCesare
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 W. Lombard Street, Baltimore, Maryland 21201, USA
Anal Biochem 294:154-60. 2001..Combining these results and the use of a simple UV-LED as excitation source, the results show an interesting potential of these two compounds in the development of lifetime base devices using synthetic probes for glucose... - One- and two-photon induced fluorescence of Pacific Blue-labeled human serum albumin deposited on different core size silver colloidsJoanna Lukomska
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
Biopolymers 81:249-55. 2006..Much stronger increases in brightness observed with two-photon excitation, compared to one-photon excitation, indicate a dominant role of enhanced local field in fluorescence enhancement on silver colloids in solutions... - Dye-labeled silver nanoshell-bright particleJian Zhang
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
J Phys Chem B 110:8986-91. 2006..Lifetime measurements support the coupling mechanism between the dye and metal shell. This study can be used to develop novel dye-labeled metal particles with bright and narrow emission bands... - Myoglobin immunoassay based on metal particle-enhanced fluorescenceEvgenia G Matveeva
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore Medical School, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
J Immunol Methods 302:26-35. 2005..We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection... - DNA crunching by a viral packaging motor: Compression of a procapsid-portal stalled Y-DNA substrateKrishanu Ray
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 N Greene St, Baltimore, MD 21201, USA
Virology 398:224-32. 2010..Torsional compression of duplex DNA is thus implicated in the mechanism of DNA translocation... - Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence images and time-resolved studies using single-molecule spectroscopyYi Fu
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Chem 78:6238-45. 2006..In addition, time-correlated single-photon counting results reveal the reduced lifetimes of single molecules tethered to silver island films... 
Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticlesChandran R Sabanayagam
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
Nucleic Acids Res 35:e13. 2007..We discuss how MEF can be used to increase the sensitivity of DNA arrays, especially for far red emitting fluorophores like Cy5, without significantly altering current microarray protocols...- Metal-enhanced single-molecule fluorescence on silver particle monomer and dimer: coupling effect between metal particlesJian Zhang
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Nano Lett 7:2101-7. 2007..Finite-difference time-domain (FDTD) calculations were employed to study the distribution of electric field near the metal monomer and dimer. The coupling effect of metal particle on the fluorescence enhancement was discussed... - Reduced blinking and long-lasting fluorescence of single fluorophores coupling to silver nanoparticlesYi Fu
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Langmuir 24:3429-33. 2008..The results show that plasmonic-controlled fluorescence can lead to a novel physical mechanism to enhance fluorescence intensity, reduce blinking, and increase photostability... - A novel method for monitoring monoclonal antibody production during cell cultureHenryk Szmacinski
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, 725 W Lombard St, Baltimore, Maryland 21201, USA
Biotechnol Bioeng 100:448-57. 2008..It is predicted that the near real time monitoring of protein products during bioprocessing will be possible with the described technology... - Metal-enhanced fluorescence of tryptophan residues in proteins: application toward label-free bioassaysHenryk Szmacinski
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, MD 21201, USA
Anal Biochem 385:358-64. 2009..The observed spectral effects of silver nanoparticles on tryptophan fluorescence indicates the possibility for the design of analytical tools for the detection of proteins without traditional labeling by extrinsic fluorophores... - Effect of plasmonic nanostructures and nanofilms on fluorescence resonance energy transferHenryk Szmacinski
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
J Biophotonics 2:243-52. 2009..To a first approximation, observed changes in FRET efficiencies were explained by an increase of the quantum yield of the donor molecules in the presence of silver islands... - Directional surface plasmon-coupled emission: application for an immunoassay in whole bloodEvgenia G Matveeva
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201, USA
Anal Biochem 344:161-7. 2005.... - Enhanced photostability of ICG in close proximity to gold colloidsChris D Geddes
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, 725 West Lombard Street, Baltimore, MD 21201, USA
Spectrochim Acta A Mol Biomol Spectrosc 59:2611-7. 2003..We showed that spatial localization of ICG near metallic gold colloids resulted in increased photostability. This suggests the use of fluorophore-metal conjugates in situations adversely affected by photobleaching... - Effects of metallic silver island films on resonance energy transfer between N,N'-(dipropyl)-tetramethyl- indocarbocyanine (Cy3)- and N,N'-(dipropyl)-tetramethyl- indodicarbocyanine (Cy5)-labeled DNAJoanna Malicka
University of Maryland Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
Biopolymers 70:595-603. 2003..These results suggest the use of metallic silver particles to increase the distances over which RET occurs in biomedical and biotechnology assays... - Fluorescence enhancement of fluorophores tethered to different sized silver colloids deposited on glass substrateJoanna Lukomska
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
Biopolymers 77:31-7. 2005..The proposed method, based on controlled hybridization with minimal amount of fluorophore labeled ss DNA, can be used to reliably estimate the fluorescence enhancements on any silver nanostructures... - Increased intensities of YOYO-1-labeled DNA oligomers near silver particlesJoseph R Lakowicz
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201, USA
Photochem Photobiol 77:604-7. 2003..Proximity to silver particles also increases the photostability of YOYO-1-DNA. These results suggest that substrates or gels containing silver particles may be used for increased sensitivity in DNA detection... - Complexation of polysaccharide and monosaccharide with thiolate boronic acid capped on silver nanoparticleJian Zhang
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 332:253-60. 2004..Luminescence intensity displayed an upward deviation increase with the concentration of dextran. The luminescence spectral change was ascribed to surface-enhanced fluorescence by the enhanced field from the aggregated metallic particles... - Wavelength-ratiometric probes for the selective detection of fluoride based on the 6-aminoquinolinium nucleus and boronic acid moietyRamachandram Badugu
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, Medical Biotechnology Center, University of Maryland School of Medicine, Baltimore, Maryland, USA
J Fluoresc 14:693-703. 2004.... - Fluorescence properties of labeled proteins near silver colloid surfacesBadri P Maliwal
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
Biopolymers 70:585-94. 2003..Because these silver island surfaces are very heterogeneous, we are hopeful that larger increases in intensity and photostability can be obtained for probes situated at an optimal distance from the ideal island surfaces... - Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island filmsJoanna Lukomska
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
Biochem Biophys Res Commun 328:78-84. 2005..In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field... - Noninvasive continuous monitoring of physiological glucose using a monosaccharide-sensing contact lensRamachandram Badugu
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Chem 76:610-8. 2004.... - Excitation and emission wavelength ratiometric cyanide-sensitive probes for physiological sensingRamachandram Badugu
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, MD 21201, USA
Anal Biochem 327:82-90. 2004..Finally we show that the affinity for monosaccharides, such as glucose or fructose, is relatively low as compared to that for cyanide, enabling the potential detection of cyanide in physiologies up to lethal levels... - Nanogold plasmon resonance-based glucose sensing. 2. Wavelength-ratiometric resonance light scatteringKadir Aslan
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, Maryland 21201, USA
Anal Chem 77:2007-14. 2005.... - Myoglobin immunoassay utilizing directional surface plasmon-coupled emissionEvgenia Matveeva
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
Anal Chem 76:6287-92. 2004..We expect SPCE to become a powerful technique for performing immunoassays for many biomarkers in surface-bound assays... - Texture analysis of fluorescence lifetime images of AT- and GC-rich regions in nucleiS Murata
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, School of Medicine, Baltimore, Maryland 21201, USA
J Histochem Cytochem 49:1443-51. 2001..Texture analysis of fluorescence intensity and lifetime images was used to quantitatively study the spatial change of condensation and separation of AT- and GC-rich DNA during the cell cycle... - Sensing oxygen through skin using a red diode laser and fluorescence lifetimesS B Bambot
Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County 21228, USA
Biosens Bioelectron 10:643-52. 1995..This work describes a paradigm for future non-invasive measurements of other analytes... - On the effect of sodium dodecyl sulfate on the structure of beta-galactosidase from Escherichia coli. A fluorescence studyS D'Auria
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, West Lombard Street, Baltimore, MD 21201 USA
J Biochem 130:13-8. 2001..The obtained results showed that the presence of SDS confers more flexibility to the protein structure, and suggest a strict relation between enzyme activity and protein flexibility... - Optical determination of glutamine using a genetically engineered proteinJ D Dattelbaum
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland, School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 291:89-95. 2001..The engineered GlnBP is a first step toward the development of a nonenzymatic biosensor capable of determining glutamine concentrations in cell cultures... - DNA dynamics: a fluorescence resonance energy transfer study using a long-lifetime metal-ligand complexJung Sook Kang
Department of Oral Biochemistry and Molecular Biology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Korea
Arch Pharm Res 25:143-50. 2002..Thus, it is suggested that the lifetime of RuBD is too short to measure diffusive processes in calf thymus DNA... - Enhanced ratiometric pH sensing using SNAFL-2 on silver island films: metal-enhanced fluorescence sensingKadir Aslan
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard St, Baltimore, Maryland 21201, USA
J Fluoresc 15:37-40. 2005..To the best of our knowledge this is the first report of enhanced ratiometric fluorescence sensing on metallic surfaces... - Monoboronic acid sensor that displays anomalous fluorescence sensitivity to glucoseHaishi Cao
Department of Chemistry, New Mexico Institute of Mining and Technology, Socorro, New Mexico 87801, USA
Org Lett 4:1503-5. 2002..This novel saccharide probe exhibits dual emission suitable for ratiometric sensing and displays a remarkable sensitivity for glucose relative to fructose and galactose... - Lateral diffusion coefficients in membranes measured by resonance energy transfer and a new algorithm for diffusion in two dimensionsJósef Kuśba
Technical University of Gdansk, Faculty of Applied Physics and Mathematics, Gdansk, Poland
Biophys J 82:1358-72. 2002..7 x 10(-7) cm(2)/s in 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) at 35 degrees C. These results demonstrated the possibility of direct measurements of lateral diffusion coefficients using microsecond decay time luminophores... - Use of surface plasmon-coupled emission to measure DNA hybridizationJoanna Malicka
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 21201, USA
J Biomol Screen 9:208-15. 2004..These results indicate that SPCE can provide highly sensitive real-time measurement of DNA hybridization... - DNA hybridization assays using metal-enhanced fluorescenceJoanna Malicka
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
Biochem Biophys Res Commun 306:213-8. 2003..These results suggest the use of silver particles as a general approach to measure DNA hybridization as a method to increase the sensitivity of DNA detection... - Submicrometer spatial resolution of metal-enhanced fluorescenceVincent J Pugh
Microcosm, Inc, 9140 Guilford Rd, Suite O, Columbia, Maryland 21046 2585, USA
Appl Spectrosc 57:1592-8. 2003..No long-range spatial ordering of the fluorescence enhancement on chemcially deposited silver nanoparticle slides was detected... - Release of the self-quenching of fluorescence near silver metallic surfacesJoseph R Lakowicz
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 320:13-20. 2003..These results suggest the use of heavily labeled proteins and metallic colloids to obtain ultrabright reagents for use in immunoassays, imaging, and other applications... - Metal particle-enhanced fluorescent immunoassays on metal mirrorsEvgenia G Matveeva
Department of Molecular Biology and Immunology, Health Science Center, University of North Texas, Fort Worth, TX 76107, USA
Anal Biochem 363:239-45. 2007..Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement... - Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particlesJoanna Malicka
Department of Chemistry, University of Gdansk, 80-952 Gdansk, Sobieskiego 18, Poland
Biopolymers 72:96-104. 2003..These results suggest that DNA arrays fabricated on substrates with silver particles can display increased sensitivity and photostability in the analysis of gene expression...
Research Grants
- Plasmon-Controlled Fluorescence and Cardiac MarkersJoseph Lakowicz; Fiscal Year: 2009..This extension of the specific aims will result in the hiring of two PhD-level research associates. ..
- Annual Meeting of the NCRR/NIBIB Principal InvestigatorsJoseph Lakowicz; Fiscal Year: 2006..5. Poster sessions and small focus groups arranged by the Principal Investigators, and 6. A closing session without NIH personnel to elect organizing committee and other discussions. ..
- CENTER FOR FLUORESCENCE SPECTROSCOPYJoseph Lakowicz; Fiscal Year: 2006..Abstract Not Provided. ..
- CFS Course on Fluorescence SpectroscopyJoseph Lakowicz; Fiscal Year: 2006..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
- Metal-Enhanced Fluorescence SensingJoseph Lakowicz; Fiscal Year: 2006..a. Develop sandwich (capture) immunoassays for PSA using anti-PSA (or anti-ACT-PSA) labeled with low and high quantum yield fluorophores. b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. ..
- Plasmon-Controlled Fluorescence and Cardiac MarkersJoseph Lakowicz; Fiscal Year: 2007..Cardiac troponin I and BMP are present at lower concentrations and their detection will drive development of nanostructures for high sensitivity detection. ..
- Metallic Surfaces and Particles in DNA AnalysisJoseph Lakowicz; Fiscal Year: 2007..B. Apply the nanostructures array for identification of class B biohazard agents. C and D. Apply the nanostructures for analysis of the cystic fibrosis transmembrane regulator gene (CFTR). ..
- Metallic Surfaces and Particles in DNA AnalysisJoseph Lakowicz; Fiscal Year: 2009..B. Apply the nanostructures array for identification of class B biohazard agents. C and D. Apply the nanostructures for analysis of the cystic fibrosis transmembrane regulator gene (CFTR). ..
- Plasmon-Controlled Fluorescence and Cardiac MarkersJoseph Lakowicz; Fiscal Year: 2009..Cardiac troponin I and BMP are present at lower concentrations and their detection will drive development of nanostructures for high sensitivity detection. ..
- Metallic Surfaces and Particles in DNA AnalysisJoseph Lakowicz; Fiscal Year: 2005..Both one-photon and multi-photon excitation will be used. We expect this project to determine how metallic particle effects on fluorescence can be used for new approaches to DNA analysis. ..
- Annual Meeting of the NCRR P41 Principal InvestigatorsJoseph Lakowicz; Fiscal Year: 2005..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
- Biohazard Detection Using Metal-Enhanced FluorescenceJoseph Lakowicz; Fiscal Year: 2003..pestis. The sensitivity of the assays will be determined by assays based on serial dilutions. The specificity will be determined against DNA from other species and strains of Yersinia. ..
- Metallic Surfaces and Particles in DNA AnalysisJoseph R Lakowicz; Fiscal Year: 2010..Each type of structure provides unique opportunities for new assay formats and can be readily available to other laboratories at low cost. ..