J R Lakowicz

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. pmc Advances in surface-enhanced fluorescence
    Joseph R Lakowicz
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Fluoresc 14:425-41. 2004
  2. pmc Radiative decay engineering 3. Surface plasmon-coupled directional emission
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 324:153-69. 2004
  3. pmc Plasmonic technology: novel approach to ultrasensitive immunoassays
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Clin Chem 51:1914-22. 2005
  4. pmc Radiative decay engineering 5: metal-enhanced fluorescence and plasmon emission
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 337:171-94. 2005
  5. pmc Silver particles enhance emission of fluorescent DNA oligomers
    Joseph R Lakowicz
    University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biotechniques 34:62-6, 68. 2003
  6. pmc Enhanced fluorescence of Cy5-labeled oligonucleotides near silver island films: a distance effect study using single molecule spectroscopy
    Yi Fu
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, Baltimore, Maryland 21201 USA
    J Phys Chem B 110:22557-62. 2006
  7. pmc Ultrabright fluorescein-labeled antibodies near silver metallic surfaces
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biopolymers 74:467-75. 2004
  8. ncbi request reprint Novel fluorescence sensing methods for high throughput screening
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Biomol Screen 5:123-32. 2000
  9. pmc Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Anal Biochem 315:57-66. 2003
  10. ncbi request reprint Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing
    Kadir Aslan
    Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Am Chem Soc 127:12115-21. 2005

Research Grants

  1. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2009
  2. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2001
  3. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2005
  4. Annual Meeting of the NCRR/NIBIB Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2006
  5. CENTER FOR FLUORESCENCE SPECTROSCOPY
    Joseph Lakowicz; Fiscal Year: 2006
  6. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2006
  7. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006
  8. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2007
  9. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2007
  10. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2009

Collaborators

Detail Information

Publications93

  1. pmc Advances in surface-enhanced fluorescence
    Joseph R Lakowicz
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Fluoresc 14:425-41. 2004
    ..We discuss a number of methods for various shaped silver particle deposition on surfaces...
  2. pmc Radiative decay engineering 3. Surface plasmon-coupled directional emission
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 324:153-69. 2004
    ..Examples showing how simple optical configurations can be used in diagnostics, sensing, or biotechnology applications are presented. Surface plasmon-coupled emission is likely to find widespread applications throughout the biosciences...
  3. pmc Plasmonic technology: novel approach to ultrasensitive immunoassays
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Clin Chem 51:1914-22. 2005
    ....
  4. pmc Radiative decay engineering 5: metal-enhanced fluorescence and plasmon emission
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 337:171-94. 2005
    ..The RP model provides a rational approach for designing fluorophore-metal configurations with the desired emissive properties and a basis for nanophotonic fluorophore technology...
  5. pmc Silver particles enhance emission of fluorescent DNA oligomers
    Joseph R Lakowicz
    University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biotechniques 34:62-6, 68. 2003
    ..Proximity to these silver particles also increases the photostability of Cy3- and Cy5-labeled oligomers. These results suggest the use of DNA array substrates with silver particles for increased sensitivity in genetic analysis...
  6. pmc Enhanced fluorescence of Cy5-labeled oligonucleotides near silver island films: a distance effect study using single molecule spectroscopy
    Yi Fu
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, Baltimore, Maryland 21201 USA
    J Phys Chem B 110:22557-62. 2006
    ..These studies illustrate the exclusive information that is otherwise hidden in ensemble measurements...
  7. pmc Ultrabright fluorescein-labeled antibodies near silver metallic surfaces
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biopolymers 74:467-75. 2004
    ..These results suggest the use of labeled antibodies conjugated to silver particles as ultrabright probes for imaging or analytical applications...
  8. ncbi request reprint Novel fluorescence sensing methods for high throughput screening
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Biomol Screen 5:123-32. 2000
    ..Sensing with a reference film provides the opportunity to internally reference HTS measurements without the need for additions to the sample. This approach can provide standardization for assays performed at different times...
  9. pmc Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Anal Biochem 315:57-66. 2003
    ..These results suggest that maximum fluorescence enhancements occur about 90A from the silver surface, a distance readily obtained by one or two layers of proteins...
  10. ncbi request reprint Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing
    Kadir Aslan
    Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Am Chem Soc 127:12115-21. 2005
    ....
  11. pmc Time-resolved fluorescence spectroscopy and imaging of DNA labeled with DAPI and Hoechst 33342 using three-photon excitation
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA
    Biophys J 72:567-78. 1997
    ..Three-photon excitation of DAPI-stained chromosomes at 970 nm was demonstrated by the power dependence in the fluorescence microscope...
  12. pmc Fluorescence enhancements on silver colloid coated surfaces
    Joanna Lukomska
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Fluoresc 14:417-23. 2004
    ..Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence...
  13. pmc Photodeposition of silver can result in metal-enhanced fluorescence
    Chris D Geddes
    University Maryland Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Appl Spectrosc 57:526-31. 2003
    ..These results demonstrate the possibility of photolithographic preparation of surfaces for enhanced fluorescence in microfluidics, medical diagnostics, and other applications...
  14. ncbi request reprint On the possibility of long-wavelength long-lifetime high-quantum-yield luminophores
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 288:62-75. 2001
    ..These results suggest the synthesis of covalently linked donor-acceptor pairs with the desirable spectral properties of long wavelength emission, high quantum yield, and moderately long lifetimes for gated detection...
  15. pmc Enhanced emission of highly labeled DNA oligomers near silver metallic surfaces
    Joanna Malicka
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Chem 75:4408-14. 2003
    ..The increased intensity did not result in increased photobleaching. These results suggest the use of substrates coated with silver particles for increased sensitivity on DNA arrays or for DNA analysis...
  16. pmc Plasmonic enhancement of single-molecule fluorescence near a silver nanoparticle
    Yi Fu
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 W Lombard Street, Baltimore, MD 21201, USA
    J Fluoresc 17:811-6. 2007
    ..These results demonstrate that the use of fluorophore-metal interactions can increase the brightness and photostability of fluorophores for single molecule detection...
  17. ncbi request reprint Saccharide sensing using gold and silver nanoparticles--a review
    Kadir Aslan
    Center for Fluorescence Spectroscopy, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Fluoresc 14:391-400. 2004
    ..Lifetime measurements were used to distinguish the contribution from the surface-enhanced fluorescence. TEM was employed to assess the aggregation of nanoparticles...
  18. pmc Metallic colloid wavelength-ratiometric scattering sensors
    David Roll
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Chem 75:3440-5. 2003
    ..This approach can be used in a wide variety of assay formats, including those commonly used with fluorescence detection...
  19. pmc Fluorescence images of DNA-bound YOYO between coupled silver particles
    Jian Zhang
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Langmuir 23:11734-9. 2007
    ..The shortened lifetime of YOYO molecules between the coupled metal particles indicated that the fluorescence was enhanced via a near-field interaction mechanism between the fluorophore and the metal nanoparticle...
  20. pmc A model for DNA detection by metal-enhanced fluorescence from immobilized silver nanoparticles on solid substrate
    Jian Zhang
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Phys Chem B 110:2387-92. 2006
    ..This result reveals that increasing the particle layer contributes to fluorescence enhancement. This novel method was used to detect DNA hybridization through both absorbance and emission spectral changes...
  21. ncbi request reprint Radiative decay engineering: biophysical and biomedical applications
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 W. Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 298:1-24. 2001
    ..As one example we predict that nearby metal surfaces can be used to increase the low intrinsic quantum yields of nucleic acids and make unlabeled DNA detectable using its intrinsic metal-enhanced fluorescence...
  22. ncbi request reprint Intrinsic fluorescence from DNA can be enhanced by metallic particles
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Biochem Biophys Res Commun 286:875-9. 2001
    ....
  23. ncbi request reprint Two-color two-photon excitation of fluorescence
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA
    Photochem Photobiol 64:632-5. 1996
    ..The amplitude of the signal depended on the polarization of each beam. 2C2P excitation can have applications in fluorescence microscopy and elsewhere when spatially localized excitation is desirable...
  24. ncbi request reprint A lifetime-based optical CO2 gas sensor with blue or red excitation and stokes or anti-stokes detection
    J Sipior
    Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201 1503, USA
    Anal Biochem 227:309-18. 1995
    ..The use of fluorescence resonance energy transfer, along with the simple entrainment procedure, allows facile change of the CO2 response range through change of the acceptor dye and the use of laser diode excitation sources...
  25. pmc Enhanced fluorescence of proteins and label-free bioassays using aluminum nanostructures
    Krishanu Ray
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, Maryland 21201, USA
    Anal Chem 81:6049-54. 2009
    ..Binding of streptavidin to the biotinylated aluminum surface was demonstrated in the presence of 100 microg/mL bovine serum albumin in the sample solution by measurements of tryptophan intensity and lifetime changes...
  26. pmc Fluorescence spectral properties of cyanine dye-labeled DNA oligomers on surfaces coated with silver particles
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 317:136-46. 2003
    ..These results suggest the use of substrates containing silver particles for increased sensitivity of DNA detection on DNA arrays...
  27. pmc Multi-wavelength immunoassays using surface plasmon-coupled emission
    Evgenia Matveeva
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biochem Biophys Res Commun 313:721-6. 2004
    ..This method of SPCE immunoassays can be readily extended to 4 or more wavelengths...
  28. pmc Oligonucleotide-displaced organic monolayer-protected silver nanoparticles and enhanced luminescence of their salted aggregates
    Jian Zhang
    Department of Biochemistry, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 330:81-6. 2004
    ..This result suggests the possible application of surface-enhanced fluorescence from metallic nanoparticle aggregation for DNA detection...
  29. pmc Single-molecule and FRET fluorescence correlation spectroscopy analyses of phage DNA packaging: colocalization of packaged phage T4 DNA ends within the capsid
    Krishanu Ray
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD 21201, USA
    J Mol Biol 395:1102-13. 2010
    ..The analysis suggests that a DNA loop, rather than a DNA end, is translocated by the packaging motor to fill the procapsid...
  30. ncbi request reprint Metal-enhanced emission from indocyanine green: a new approach to in vivo imaging
    Joanna Malicka
    University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, 725 W Lombard Street, Baltimore, Maryland 21201 1503, USA
    J Biomed Opt 8:472-8. 2003
    ..Since the rate of photobleaching is not increased, our results suggest that ICG-silver particle complexes can yield at least 20-fold more photons per ICG molecule for improved medical imaging...
  31. ncbi request reprint On the possibility of evanescent wave excitation distal from a solid-liquid interface using light quenching
    J R Lakowicz
    Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201 1503, USA
    Photochem Photobiol 64:636-41. 1996
    ....
  32. ncbi request reprint Long-wavelength long-lifetime luminophores
    B P Maliwal
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 21201, USA
    Anal Chem 73:4277-85. 2001
    ..Luminophores with long-wavelength emission and long lifetimes can have numerous applications in biophysics, clinical diagnostics, DNA analysis, and drug discovery...
  33. ncbi request reprint Fluorescence of reduced nicotinamides using one- and two-photon excitation
    B Kierdaszuk
    Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201, USA
    Biophys Chem 62:1-13. 1996
    ....
  34. pmc Roughened silver electrodes for use in metal-enhanced fluorescence
    Chris D Geddes
    Center for Fluorescence Spectroscopy, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, MD 21201, USA
    Spectrochim Acta A Mol Biomol Spectrosc 60:1977-83. 2004
    ..To the best of our knowledge this is the first report of roughened silver electrodes for metal-enhanced fluorescence...
  35. ncbi request reprint Metal-enhanced fluorescence immunoassays using total internal reflection and silver island-coated surfaces
    Evgenia Matveeva
    Center for Fluorescence Spectroscopy, Department of Biochemistry Molecular Biology, University of Maryland at Baltimore Medical School, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 334:303-11. 2004
    ..The limited evanescent wave excitation volume makes it possible to minimize the background signal and use the immunoassay with no need for any washing steps...
  36. ncbi request reprint Surface-plasmon-coupled emission: new technology for studying molecular processes
    Zygmunt Gryczynski
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, Baltimore, Maryland 21201, USA
    Methods Cell Biol 75:73-104. 2004
  37. ncbi request reprint Ophthalmic glucose monitoring using disposable contact lenses--a review
    Ramachandram Badugu
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Fluoresc 14:617-33. 2004
    ....
  38. pmc Fluorescent avidin-bound silver particle: a strategy for single target molecule detection on a cell membrane
    Jian Zhang
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Chem 81:883-9. 2009
    ..On the basis of this curve, we expect to develop an approach that can be used to quantify the amount of target molecules on the cell surfaces using the cell intensity and lifetime images at the single cell level...
  39. ncbi request reprint Fast and slow deposition of silver nanorods on planar surfaces: application to metal-enhanced fluorescence
    Kadir Aslan
    Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, USA
    J Phys Chem B 109:3157-62. 2005
    ..Subsequently, these new silver nanorod preparation procedures are likely to find a common place in MEF, as they are a quicker and much cheaper alternative as compared to surfaces fabricated by traditional nanolithographic techniques...
  40. ncbi request reprint Metal-enhanced fluorescence solution-based sensing platform
    Kadir Asian
    Institute of Fluorescence, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland, USA
    J Fluoresc 14:677-9. 2004
    ..In this short, note, we show how SiO2-coated silver colloids, indeed provide for a solution based enhanced fluorescence sensing platform with a 3-5 fold enhancement typically observed...
  41. ncbi request reprint Metal-enhanced chemiluminescence
    Mustafa H Chowdhury
    Center for Fluorescence Spectroscopy, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, MD 21201, USA
    J Fluoresc 16:295-9. 2006
    ..This finding is of significance towards our understanding of fluorophore-metal interactions, a relatively new near-field fluorescence concept, recently named metal-enhanced fluorescence and also radiative decay engineering...
  42. ncbi request reprint Plastic versus glass support for an immunoassay on metal-coated surfaces in optically dense samples utilizing directional surface plasmon-coupled emission
    Evgenia G Matveeva
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Fluoresc 15:865-71. 2005
    ..We believe that plastic substrates can be readily used in any SPCE assay, with only marginally lower total signal compared to high-quality glass slides...
  43. ncbi request reprint Radiative decay engineering. 2. Effects of Silver Island films on fluorescence intensity, lifetimes, and resonance energy transfer
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 301:261-77. 2002
    ..These results demonstrate that metallic particles can be used to modify the emission from intrinsic and extrinsic fluorophores in biochemical systems...
  44. pmc Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, MD 21201, USA
    Biochem Biophys Res Commun 294:886-92. 2002
    ..6 to 63 A. These results suggest the use of silver island films for detecting long range proximity between biomolecules and for biotechnology applications based on RET...
  45. ncbi request reprint Emerging applications of fluorescence spectroscopy to cellular imaging: lifetime imaging, metal-ligand probes, multi-photon excitation and light quenching
    J R Lakowicz
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore
    Scanning Microsc Suppl 10:213-24. 1996
    ..This use of non-absorbed light to modify emission can have wide reaching applications in cellular imaging...
  46. ncbi request reprint Frequency-domain fluorescence microscopy with the LED as a light source
    P Herman
    University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Microsc 203:176-81. 2001
    ..The ability to select an excitation source for a given fluorophore and low price make such an excitation source even more practical...
  47. ncbi request reprint Fluorescence lifetime characterization of novel low-pH probes
    H J Lin
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 294:118-25. 2001
    ..These results indicate that LysoSensor DND-160 is a promising probe for lifetime imaging to determine lysosomal pH...
  48. pmc Plasmon-enhanced fluorescence from single fluorophores end-linked to gold nanorods
    Yi Fu
    University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Am Chem Soc 132:5540-1. 2010
    ..The bioconjugated nanoantenna structure implicates an approach that is more convenient and amenable to integration for photonic, optoelectronic, and biotechnological applications...
  49. pmc Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles
    Joanna Malicka
    University of Maryland Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 W Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 315:160-9. 2003
    ..These results suggest the use of metal-enhanced RET in PCR, hybridization, and other DNA assays, and the possibility of controlling energy transfer by the distance between silver surfaces...
  50. pmc Single cell fluorescence imaging using metal plasmon-coupled probe
    Jian Zhang
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Bioconjug Chem 18:800-5. 2007
    ..PCPs were conjugated on HEK 293 A cells. Imaging results demonstrate that cells labeled by PCPs were 20-fold brighter than those by free labeled con A...
  51. ncbi request reprint Plasmon light scattering in biology and medicine: new sensing approaches, visions and perspectives
    Kadir Aslan
    Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD 21201, USA
    Curr Opin Chem Biol 9:538-44. 2005
    ....
  52. doi request reprint Multiphoton excitation of lanthanides
    J R Lakowicz
    Center for Fluorescence Spectroscopy, Deptartment of Biochemistry and Molecular Biology, School of Medicine, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Chemphyschem 2:247-52. 2001
    ..This suggests the use of lanthanides as calcium analogues in multiphoton microscopy. The spectrum shows the normalized two-photon excitation emission spectra (λex = 796 nm) of Eu(3+) with a resolution of about 8 nm...
  53. ncbi request reprint Evaluation of two synthetic glucose probes for fluorescence-lifetime-based sensing
    N DiCesare
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 W. Lombard Street, Baltimore, Maryland 21201, USA
    Anal Biochem 294:154-60. 2001
    ..Combining these results and the use of a simple UV-LED as excitation source, the results show an interesting potential of these two compounds in the development of lifetime base devices using synthetic probes for glucose...
  54. ncbi request reprint Fluorescence properties of albumin blue 633 and 670 in plasma and whole blood
    O O Abugo
    University of Maryland at Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Biomed Opt 6:359-65. 2001
    ....
  55. pmc Reduced blinking and long-lasting fluorescence of single fluorophores coupling to silver nanoparticles
    Yi Fu
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Langmuir 24:3429-33. 2008
    ..The results show that plasmonic-controlled fluorescence can lead to a novel physical mechanism to enhance fluorescence intensity, reduce blinking, and increase photostability...
  56. ncbi request reprint One- and two-photon induced fluorescence of Pacific Blue-labeled human serum albumin deposited on different core size silver colloids
    Joanna Lukomska
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biopolymers 81:249-55. 2006
    ..Much stronger increases in brightness observed with two-photon excitation, compared to one-photon excitation, indicate a dominant role of enhanced local field in fluorescence enhancement on silver colloids in solutions...
  57. pmc DNA crunching by a viral packaging motor: Compression of a procapsid-portal stalled Y-DNA substrate
    Krishanu Ray
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 N Greene St, Baltimore, MD 21201, USA
    Virology 398:224-32. 2010
    ..Torsional compression of duplex DNA is thus implicated in the mechanism of DNA translocation...
  58. pmc Effect of plasmonic nanostructures and nanofilms on fluorescence resonance energy transfer
    Henryk Szmacinski
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biophotonics 2:243-52. 2009
    ..To a first approximation, observed changes in FRET efficiencies were explained by an increase of the quantum yield of the donor molecules in the presence of silver islands...
  59. pmc Dye-labeled silver nanoshell-bright particle
    Jian Zhang
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Phys Chem B 110:8986-91. 2006
    ..Lifetime measurements support the coupling mechanism between the dye and metal shell. This study can be used to develop novel dye-labeled metal particles with bright and narrow emission bands...
  60. ncbi request reprint Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence images and time-resolved studies using single-molecule spectroscopy
    Yi Fu
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Chem 78:6238-45. 2006
    ..In addition, time-correlated single-photon counting results reveal the reduced lifetimes of single molecules tethered to silver island films...
  61. pmc Metal-enhanced fluorescence of tryptophan residues in proteins: application toward label-free bioassays
    Henryk Szmacinski
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, MD 21201, USA
    Anal Biochem 385:358-64. 2009
    ..The observed spectral effects of silver nanoparticles on tryptophan fluorescence indicates the possibility for the design of analytical tools for the detection of proteins without traditional labeling by extrinsic fluorophores...
  62. pmc A novel method for monitoring monoclonal antibody production during cell culture
    Henryk Szmacinski
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, 725 W Lombard St, Baltimore, Maryland 21201, USA
    Biotechnol Bioeng 100:448-57. 2008
    ..It is predicted that the near real time monitoring of protein products during bioprocessing will be possible with the described technology...
  63. pmc Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles
    Chandran R Sabanayagam
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Nucleic Acids Res 35:e13. 2007
    ..We discuss how MEF can be used to increase the sensitivity of DNA arrays, especially for far red emitting fluorophores like Cy5, without significantly altering current microarray protocols...
  64. pmc Metal-enhanced single-molecule fluorescence on silver particle monomer and dimer: coupling effect between metal particles
    Jian Zhang
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Nano Lett 7:2101-7. 2007
    ..Finite-difference time-domain (FDTD) calculations were employed to study the distribution of electric field near the metal monomer and dimer. The coupling effect of metal particle on the fluorescence enhancement was discussed...
  65. ncbi request reprint Complexation of polysaccharide and monosaccharide with thiolate boronic acid capped on silver nanoparticle
    Jian Zhang
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 332:253-60. 2004
    ..Luminescence intensity displayed an upward deviation increase with the concentration of dextran. The luminescence spectral change was ascribed to surface-enhanced fluorescence by the enhanced field from the aggregated metallic particles...
  66. ncbi request reprint Directional surface plasmon-coupled emission: application for an immunoassay in whole blood
    Evgenia G Matveeva
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201, USA
    Anal Biochem 344:161-7. 2005
    ....
  67. pmc Effects of metallic silver island films on resonance energy transfer between N,N'-(dipropyl)-tetramethyl- indocarbocyanine (Cy3)- and N,N'-(dipropyl)-tetramethyl- indodicarbocyanine (Cy5)-labeled DNA
    Joanna Malicka
    University of Maryland Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biopolymers 70:595-603. 2003
    ..These results suggest the use of metallic silver particles to increase the distances over which RET occurs in biomedical and biotechnology assays...
  68. pmc Fluorescence enhancement of fluorophores tethered to different sized silver colloids deposited on glass substrate
    Joanna Lukomska
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biopolymers 77:31-7. 2005
    ..The proposed method, based on controlled hybridization with minimal amount of fluorophore labeled ss DNA, can be used to reliably estimate the fluorescence enhancements on any silver nanostructures...
  69. ncbi request reprint Myoglobin immunoassay utilizing directional surface plasmon-coupled emission
    Evgenia Matveeva
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Chem 76:6287-92. 2004
    ..We expect SPCE to become a powerful technique for performing immunoassays for many biomarkers in surface-bound assays...
  70. pmc Enhanced photostability of ICG in close proximity to gold colloids
    Chris D Geddes
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, 725 West Lombard Street, Baltimore, MD 21201, USA
    Spectrochim Acta A Mol Biomol Spectrosc 59:2611-7. 2003
    ..We showed that spatial localization of ICG near metallic gold colloids resulted in increased photostability. This suggests the use of fluorophore-metal conjugates in situations adversely affected by photobleaching...
  71. ncbi request reprint Wavelength-ratiometric probes for the selective detection of fluoride based on the 6-aminoquinolinium nucleus and boronic acid moiety
    Ramachandram Badugu
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, Medical Biotechnology Center, University of Maryland School of Medicine, Baltimore, Maryland, USA
    J Fluoresc 14:693-703. 2004
    ....
  72. pmc Fluorescence properties of labeled proteins near silver colloid surfaces
    Badri P Maliwal
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biopolymers 70:585-94. 2003
    ..Because these silver island surfaces are very heterogeneous, we are hopeful that larger increases in intensity and photostability can be obtained for probes situated at an optimal distance from the ideal island surfaces...
  73. ncbi request reprint Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films
    Joanna Lukomska
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biochem Biophys Res Commun 328:78-84. 2005
    ..In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field...
  74. ncbi request reprint Excitation and emission wavelength ratiometric cyanide-sensitive probes for physiological sensing
    Ramachandram Badugu
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, MD 21201, USA
    Anal Biochem 327:82-90. 2004
    ..Finally we show that the affinity for monosaccharides, such as glucose or fructose, is relatively low as compared to that for cyanide, enabling the potential detection of cyanide in physiologies up to lethal levels...
  75. ncbi request reprint Nanogold plasmon resonance-based glucose sensing. 2. Wavelength-ratiometric resonance light scattering
    Kadir Aslan
    Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, Maryland 21201, USA
    Anal Chem 77:2007-14. 2005
    ....
  76. ncbi request reprint Myoglobin immunoassay based on metal particle-enhanced fluorescence
    Evgenia G Matveeva
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore Medical School, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA
    J Immunol Methods 302:26-35. 2005
    ..We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection...
  77. ncbi request reprint Noninvasive continuous monitoring of physiological glucose using a monosaccharide-sensing contact lens
    Ramachandram Badugu
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Chem 76:610-8. 2004
    ....
  78. pmc Increased intensities of YOYO-1-labeled DNA oligomers near silver particles
    Joseph R Lakowicz
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201, USA
    Photochem Photobiol 77:604-7. 2003
    ..Proximity to silver particles also increases the photostability of YOYO-1-DNA. These results suggest that substrates or gels containing silver particles may be used for increased sensitivity in DNA detection...
  79. ncbi request reprint Texture analysis of fluorescence lifetime images of AT- and GC-rich regions in nuclei
    S Murata
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, School of Medicine, Baltimore, Maryland 21201, USA
    J Histochem Cytochem 49:1443-51. 2001
    ..Texture analysis of fluorescence intensity and lifetime images was used to quantitatively study the spatial change of condensation and separation of AT- and GC-rich DNA during the cell cycle...
  80. ncbi request reprint Optical determination of glutamine using a genetically engineered protein
    J D Dattelbaum
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland, School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 291:89-95. 2001
    ..The engineered GlnBP is a first step toward the development of a nonenzymatic biosensor capable of determining glutamine concentrations in cell cultures...
  81. ncbi request reprint On the effect of sodium dodecyl sulfate on the structure of beta-galactosidase from Escherichia coli. A fluorescence study
    S D'Auria
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, West Lombard Street, Baltimore, MD 21201 USA
    J Biochem 130:13-8. 2001
    ..The obtained results showed that the presence of SDS confers more flexibility to the protein structure, and suggest a strict relation between enzyme activity and protein flexibility...
  82. ncbi request reprint Sensing oxygen through skin using a red diode laser and fluorescence lifetimes
    S B Bambot
    Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County 21228, USA
    Biosens Bioelectron 10:643-52. 1995
    ..This work describes a paradigm for future non-invasive measurements of other analytes...
  83. ncbi request reprint Plasmon-controlled fluorescence towards high-sensitivity optical sensing
    K Ray
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 W Lombard St, Baltimore, MD, 21201, USA
    Adv Biochem Eng Biotechnol 116:29-72. 2009
    ..Recent research combining plasmonics and fluorescence suggest that PCF could lead to new classes of experimental procedures, novel probes, bioassays, and devices...
  84. ncbi request reprint Submicrometer spatial resolution of metal-enhanced fluorescence
    Vincent J Pugh
    Microcosm, Inc, 9140 Guilford Rd, Suite O, Columbia, Maryland 21046 2585, USA
    Appl Spectrosc 57:1592-8. 2003
    ..No long-range spatial ordering of the fluorescence enhancement on chemcially deposited silver nanoparticle slides was detected...
  85. pmc Use of surface plasmon-coupled emission to measure DNA hybridization
    Joanna Malicka
    Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 21201, USA
    J Biomol Screen 9:208-15. 2004
    ..These results indicate that SPCE can provide highly sensitive real-time measurement of DNA hybridization...
  86. pmc Release of the self-quenching of fluorescence near silver metallic surfaces
    Joseph R Lakowicz
    Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 320:13-20. 2003
    ..These results suggest the use of heavily labeled proteins and metallic colloids to obtain ultrabright reagents for use in immunoassays, imaging, and other applications...
  87. ncbi request reprint Metal particle-enhanced fluorescent immunoassays on metal mirrors
    Evgenia G Matveeva
    Department of Molecular Biology and Immunology, Health Science Center, University of North Texas, Fort Worth, TX 76107, USA
    Anal Biochem 363:239-45. 2007
    ..Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement...
  88. pmc DNA hybridization assays using metal-enhanced fluorescence
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA
    Biochem Biophys Res Commun 306:213-8. 2003
    ..These results suggest the use of silver particles as a general approach to measure DNA hybridization as a method to increase the sensitivity of DNA detection...
  89. ncbi request reprint Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles
    Joanna Malicka
    Department of Chemistry, University of Gdansk, 80 952 Gdansk, Sobieskiego 18, Poland
    Biopolymers 72:96-104. 2003
    ..These results suggest that DNA arrays fabricated on substrates with silver particles can display increased sensitivity and photostability in the analysis of gene expression...
  90. ncbi request reprint Enhanced ratiometric pH sensing using SNAFL-2 on silver island films: metal-enhanced fluorescence sensing
    Kadir Aslan
    Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard St, Baltimore, Maryland 21201, USA
    J Fluoresc 15:37-40. 2005
    ..To the best of our knowledge this is the first report of enhanced ratiometric fluorescence sensing on metallic surfaces...
  91. ncbi request reprint DNA dynamics: a fluorescence resonance energy transfer study using a long-lifetime metal-ligand complex
    Jung Sook Kang
    Department of Oral Biochemistry and Molecular Biology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Korea
    Arch Pharm Res 25:143-50. 2002
    ..Thus, it is suggested that the lifetime of RuBD is too short to measure diffusive processes in calf thymus DNA...
  92. ncbi request reprint Monoboronic acid sensor that displays anomalous fluorescence sensitivity to glucose
    Haishi Cao
    Department of Chemistry, New Mexico Institute of Mining and Technology, Socorro, New Mexico 87801, USA
    Org Lett 4:1503-5. 2002
    ..This novel saccharide probe exhibits dual emission suitable for ratiometric sensing and displays a remarkable sensitivity for glucose relative to fructose and galactose...
  93. pmc Lateral diffusion coefficients in membranes measured by resonance energy transfer and a new algorithm for diffusion in two dimensions
    Jósef Kuśba
    Technical University of Gdansk, Faculty of Applied Physics and Mathematics, Gdansk, Poland
    Biophys J 82:1358-72. 2002
    ..7 x 10(-7) cm(2)/s in 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) at 35 degrees C. These results demonstrated the possibility of direct measurements of lateral diffusion coefficients using microsecond decay time luminophores...

Research Grants29

  1. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2009
    ..This extension of the specific aims will result in the hiring of two PhD-level research associates. ..
  2. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2001
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  3. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2005
    ..a. Develop sandwich (capture) immunoassays for PSA using anti-PSA (or anti-ACT-PSA) labeled with low and high quantum yield fluorophores. b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. ..
  4. Annual Meeting of the NCRR/NIBIB Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2006
    ..5. Poster sessions and small focus groups arranged by the Principal Investigators, and 6. A closing session without NIH personnel to elect organizing committee and other discussions. ..
  5. CENTER FOR FLUORESCENCE SPECTROSCOPY
    Joseph Lakowicz; Fiscal Year: 2006
    ..Abstract Not Provided. ..
  6. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2006
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  7. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006
    ..a. Develop sandwich (capture) immunoassays for PSA using anti-PSA (or anti-ACT-PSA) labeled with low and high quantum yield fluorophores. b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. ..
  8. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2007
    ..Cardiac troponin I and BMP are present at lower concentrations and their detection will drive development of nanostructures for high sensitivity detection. ..
  9. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2007
    ..B. Apply the nanostructures array for identification of class B biohazard agents. C and D. Apply the nanostructures for analysis of the cystic fibrosis transmembrane regulator gene (CFTR). ..
  10. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2009
    ..B. Apply the nanostructures array for identification of class B biohazard agents. C and D. Apply the nanostructures for analysis of the cystic fibrosis transmembrane regulator gene (CFTR). ..
  11. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2009
    ..Cardiac troponin I and BMP are present at lower concentrations and their detection will drive development of nanostructures for high sensitivity detection. ..
  12. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2005
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  13. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2005
    ..Both one-photon and multi-photon excitation will be used. We expect this project to determine how metallic particle effects on fluorescence can be used for new approaches to DNA analysis. ..
  14. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2005
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  15. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2002
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  16. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2003
    ..Both one-photon and multi-photon excitation will be used. We expect this project to determine how metallic particle effects on fluorescence can be used for new approaches to DNA analysis. ..
  17. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2002
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  18. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2002
    ..a. Develop sandwich (capture) immunoassays for PSA using anti-PSA (or anti-ACT-PSA) labeled with low and high quantum yield fluorophores. b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. ..
  19. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2003
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  20. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2003
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  21. Biohazard Detection Using Metal-Enhanced Fluorescence
    Joseph Lakowicz; Fiscal Year: 2003
    ..pestis. The sensitivity of the assays will be determined by assays based on serial dilutions. The specificity will be determined against DNA from other species and strains of Yersinia. ..
  22. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2003
    ..a. Develop sandwich (capture) immunoassays for PSA using anti-PSA (or anti-ACT-PSA) labeled with low and high quantum yield fluorophores. b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. ..
  23. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2004
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  24. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2004
    ..Both one-photon and multi-photon excitation will be used. We expect this project to determine how metallic particle effects on fluorescence can be used for new approaches to DNA analysis. ..
  25. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2004
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  26. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2004
    ..a. Develop sandwich (capture) immunoassays for PSA using anti-PSA (or anti-ACT-PSA) labeled with low and high quantum yield fluorophores. b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. ..
  27. Metallic Surfaces and Particles in DNA Analysis
    Joseph R Lakowicz; Fiscal Year: 2010
    ..Each type of structure provides unique opportunities for new assay formats and can be readily available to other laboratories at low cost. ..