DAVID KUSNER

Summary

Affiliation: University of Iowa
Country: USA

Publications

  1. ncbi request reprint Fc gamma receptor-mediated activation of phospholipase D regulates macrophage phagocytosis of IgG-opsonized particles
    D J Kusner
    Department of Medicine, Inflammation Program, University of Iowa, Veterans Affairs Medical Center, Iowa City 52242, USA
    J Immunol 162:2266-74. 1999
  2. ncbi request reprint ATP-induced killing of virulent Mycobacterium tuberculosis within human macrophages requires phospholipase D
    D J Kusner
    Department of Medicine, Inflammation Program, Graduate Program in Immunology, University of Iowa, Iowa City 52242, USA
    J Immunol 164:379-88. 2000
  3. ncbi request reprint Regulation of phospholipase D activity by actin. Actin exerts bidirectional modulation of Mammalian phospholipase D activity in a polymerization-dependent, isoform-specific manner
    David J Kusner
    Department of Internal Medicine, Division of Infectious Diseases, Inflammation Program, University of Iowa and Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA
    J Biol Chem 277:50683-92. 2002
  4. ncbi request reprint The regulation of dendritic cell function by calcium-signaling and its inhibition by microbial pathogens
    S F Connolly
    Department of Medicine, The Graduate Program in Immunology, The University of Iowa, Iowa City, IA 52242, USA
    Immunol Res 39:115-27. 2007
  5. ncbi request reprint Evolutionary conservation of physical and functional interactions between phospholipase D and actin
    David J Kusner
    Department of Internal Medicine, University of Iowa Carrer College of Medicine and VA Medical Center, Iowa City, IA 52242, USA
    Arch Biochem Biophys 412:231-41. 2003
  6. ncbi request reprint The localization and activity of sphingosine kinase 1 are coordinately regulated with actin cytoskeletal dynamics in macrophages
    David J Kusner
    Inflammation Program, Division of Infectious Diseases, Department of Internal Medicine, University of Iowa Carver College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa 52245, USA
    J Biol Chem 282:23147-62. 2007
  7. ncbi request reprint Mechanisms of mycobacterial persistence in tuberculosis
    David J Kusner
    Inflammation Program and Division of Infectious Diseases, Department of Internal Medicine and Physiology, Graduate Programs in Immunology and Molecular Biology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
    Clin Immunol 114:239-47. 2005
  8. ncbi request reprint Sphingosine kinase 1 (SK1) is recruited to nascent phagosomes in human macrophages: inhibition of SK1 translocation by Mycobacterium tuberculosis
    Christopher R Thompson
    Inflammation Program, University of Iowa Carver College of Medicine, Coralville, IA 52241, USA
    J Immunol 174:3551-61. 2005
  9. ncbi request reprint Calmodulin kinase II regulates the maturation and antigen presentation of human dendritic cells
    Tara L Herrmann
    Department of Internal Medicine, University of Iowa Carver College of Medicine, Coralville, IA 52241, and Iowa City Veterans Affairs Medical Center, Iowa City, IA, USA
    J Leukoc Biol 78:1397-407. 2005
  10. ncbi request reprint Assay of phospholipase D activity in cell-free systems
    Shankar S Iyer
    Inflammation Program, Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, USA
    Methods Mol Biol 332:281-98. 2006

Research Grants

  1. Phospholipase D and the Actin Cytoskeleton
    DAVID KUSNER; Fiscal Year: 2005
  2. Lung Host Pathogen Interactions Research
    DAVID KUSNER; Fiscal Year: 2003
  3. Inhibition of Macrophage Innate Immunity in Tuberculosis
    WILLIAM NAUSEEF; Fiscal Year: 2007

Collaborators

Detail Information

Publications15

  1. ncbi request reprint Fc gamma receptor-mediated activation of phospholipase D regulates macrophage phagocytosis of IgG-opsonized particles
    D J Kusner
    Department of Medicine, Inflammation Program, University of Iowa, Veterans Affairs Medical Center, Iowa City 52242, USA
    J Immunol 162:2266-74. 1999
    ....
  2. ncbi request reprint ATP-induced killing of virulent Mycobacterium tuberculosis within human macrophages requires phospholipase D
    D J Kusner
    Department of Medicine, Inflammation Program, Graduate Program in Immunology, University of Iowa, Iowa City 52242, USA
    J Immunol 164:379-88. 2000
    ..These results demonstrate that ATPe promotes killing of virulent M. tuberculosis within infected human macrophages and strongly suggest that activation of PLD plays a key role in this process...
  3. ncbi request reprint Regulation of phospholipase D activity by actin. Actin exerts bidirectional modulation of Mammalian phospholipase D activity in a polymerization-dependent, isoform-specific manner
    David J Kusner
    Department of Internal Medicine, Division of Infectious Diseases, Inflammation Program, University of Iowa and Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA
    J Biol Chem 277:50683-92. 2002
    ..These data are consistent with a model in which the physical state of the actin cytoskeleton is a critical determinant of its regulation of PLD activity...
  4. ncbi request reprint The regulation of dendritic cell function by calcium-signaling and its inhibition by microbial pathogens
    S F Connolly
    Department of Medicine, The Graduate Program in Immunology, The University of Iowa, Iowa City, IA 52242, USA
    Immunol Res 39:115-27. 2007
    ..Restoration of functional Ca2+ signaling in DC may provide a novel mechanism to enhance therapy and promote vaccine efficacy to infectious diseases, including tuberculosis...
  5. ncbi request reprint Evolutionary conservation of physical and functional interactions between phospholipase D and actin
    David J Kusner
    Department of Internal Medicine, University of Iowa Carrer College of Medicine and VA Medical Center, Iowa City, IA 52242, USA
    Arch Biochem Biophys 412:231-41. 2003
    ..Thus, physical and functional interactions between PLD and actin in PLD family members from bacteria to mammals are highly conserved throughout evolution...
  6. ncbi request reprint The localization and activity of sphingosine kinase 1 are coordinately regulated with actin cytoskeletal dynamics in macrophages
    David J Kusner
    Inflammation Program, Division of Infectious Diseases, Department of Internal Medicine, University of Iowa Carver College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa 52245, USA
    J Biol Chem 282:23147-62. 2007
    ..Thus, the localization and activity of SK1 are coordinately regulated with actin dynamics during macrophage activation...
  7. ncbi request reprint Mechanisms of mycobacterial persistence in tuberculosis
    David J Kusner
    Inflammation Program and Division of Infectious Diseases, Department of Internal Medicine and Physiology, Graduate Programs in Immunology and Molecular Biology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
    Clin Immunol 114:239-47. 2005
    ..Pharmacologic or physiologic reversal of this inhibition of macrophage Ca(2+) signaling restores the normal sequence of phagosome maturation, resulting in decreased intracellular viability of M. tuberculosis...
  8. ncbi request reprint Sphingosine kinase 1 (SK1) is recruited to nascent phagosomes in human macrophages: inhibition of SK1 translocation by Mycobacterium tuberculosis
    Christopher R Thompson
    Inflammation Program, University of Iowa Carver College of Medicine, Coralville, IA 52241, USA
    J Immunol 174:3551-61. 2005
    ..These data are consistent with a model in which M.tb inhibits both the activation and phagosomal translocation of SK1 to block the localized Ca2+ transients required for phagosome maturation...
  9. ncbi request reprint Calmodulin kinase II regulates the maturation and antigen presentation of human dendritic cells
    Tara L Herrmann
    Department of Internal Medicine, University of Iowa Carver College of Medicine, Coralville, IA 52241, and Iowa City Veterans Affairs Medical Center, Iowa City, IA, USA
    J Leukoc Biol 78:1397-407. 2005
    ..These data support a model in which CamK II regulates critical stages of the maturation and antigen presentation capacity of human DC, particularly in response to stimulation via phagocytosis...
  10. ncbi request reprint Assay of phospholipase D activity in cell-free systems
    Shankar S Iyer
    Inflammation Program, Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, USA
    Methods Mol Biol 332:281-98. 2006
    ..This assay system is used to illustrate the interactions of PLD1 with specific membrane domains and their associated filamentous and monomeric actin components...
  11. ncbi request reprint Cutting edge: Mycobacterium tuberculosis blocks Ca2+ signaling and phagosome maturation in human macrophages via specific inhibition of sphingosine kinase
    Zulfiqar A Malik
    Inflammation Program, Graduate Program in Immunology, University of Iowa and Veterans Affairs Medical Center, Iowa City, IA 52242, USA
    J Immunol 170:2811-5. 2003
    ..Thus, inhibition of sphingosine kinase directly contributes to survival of Mtb within human MPs and represents a novel molecular mechanism of pathogenesis...
  12. doi request reprint Coordinate regulation of sphingosine kinase and actin dynamics
    Shankar S Iyer
    Physiology and Biophysics, and the Graduate Program in Immunology, University of Iowa Carver College of Medicine and Iowa City Veterans Affairs Medical Centre, Iowa City, IA, USA
    Methods Mol Biol 531:347-61. 2009
    ..Here, we describe the approach we used to investigate regulation of SK1. This provides general methods that can be used to examine the role of actin in regulating enzyme activity in macrophages and other myeloid cells...
  13. ncbi request reprint Phospholipase D1 regulates phagocyte adhesion
    Shankar S Iyer
    Inflammation Program, Division of Infectious Diseases, Department of Internal Medicine, University of Iowa Carver College of Medicine, 2501 Crosspark Road, Iowa City, IA 52242, USA
    J Immunol 176:3686-96. 2006
    ..These data support the hypotheses that adhesion stimulates PLD activity, and that PLD1 regulates the initial stages of phagocyte adhesion. Stimulation of PLD activity may promote adhesion-dependent phagocyte effector responses...
  14. ncbi request reprint Phospholipases D1 and D2 coordinately regulate macrophage phagocytosis
    Shankar S Iyer
    Inflammation Program, Division of Infectious Diseases, Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City 52241, USA
    J Immunol 173:2615-23. 2004
    ..Human myelomonocytic cell lines accurately model PLD-dependent signal transduction events required for phagocytosis, but the heterologous COS cell system does not...
  15. ncbi request reprint MHC Class II levels and intracellular localization in human dendritic cells are regulated by calmodulin kinase II
    Tara L Herrmann
    The Inflammation Program, Division of Infectious Diseases, University of Iowa Carver College of Medicine, 200 Hawkins Dr, SW 54 8, GH, Iowa City, IA 52242, USA
    J Leukoc Biol 82:686-99. 2007
    ..These pathways are likely important to the physiologic regulation of MHC Class II as well as to its dysregulation in disease states associated with altered CaMKII function...

Research Grants13

  1. Phospholipase D and the Actin Cytoskeleton
    DAVID KUSNER; Fiscal Year: 2005
    ..These studies will further both our specific knowledge of signal transduction mechanisms that regulate inflammation as well as our more general understanding of the spatial and temporal determinants of cellular activation and motility. ..
  2. Lung Host Pathogen Interactions Research
    DAVID KUSNER; Fiscal Year: 2003
    ..abstract not provided ..
  3. Inhibition of Macrophage Innate Immunity in Tuberculosis
    WILLIAM NAUSEEF; Fiscal Year: 2007
    ..A genetic approach of screening a transposon mutant library of Mtb and a biochemical approach of direct assessment of subcellular fractions of Mtb for effects on macrophage SK-andCa2+-mediated signal transduction will be undertaken. ..