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| B T KurienSummaryAffiliation: University of Oklahoma Health Sciences Center Country: USA Publications
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Publications
Mechanized syringe homogenization of human and animal tissuesBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 825 NE 13th Street, OK 73104, USA
Assay Drug Dev Technol 2:308-12. 2004..Use of the Bio-Mixer 1200 to homogenize animal or human tissue precludes the need to stay in the cold room as is the case with the other hands-on homogenization methods available, in addition to freeing up time for other experiments...
Heat-solubilized curry spice curcumin inhibits antibody-antigen interaction in in vitro studies: a possible therapy to alleviate autoimmune disordersBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
Mol Nutr Food Res 54:1202-9. 2010..In addition, the non-toxic curcumin could serve as a new protein stain in SDS-PAGE even though it is less sensitive than the Coomassie system which involves toxic chemicals...- Determination of prolidase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometryBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
Anal Biochem 331:224-9. 2004..Using this method we have unambiguously identified subjects with homozygous or heterozygous prolidase deficiency. In addition to the advantage of rapid sample preparation time, this method is highly specific, reproducible, and sensitive... - Protein blotting: a reviewBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
J Immunol Methods 274:1-15. 2003 - Experimental animal urine collection: a reviewBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, OK 73104, USA
Lab Anim 38:333-61. 2004..A continuous refinement in the procedures for collecting urine from experimental animals will be the most efficient way of proceeding in obtaining pure urine specimens for obtaining reliable research data... - Heat-mediated, ultra-rapid electrophoretic transfer of high and low molecular weight proteins to nitrocellulose membranesBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
J Immunol Methods 266:127-33. 2002..In summary, this procedure is very rapid, avoids the use of methanol and is particularly useful for the transfer of high molecular weight proteins... - Extraction of nucleic acid fragments from gelsBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, 73104, USA
Anal Biochem 302:1-9. 2002 - In vitro modification of solid phase multiple antigenic peptides/autoantigens with 4-hydroxy-2-nonenal (HNE) provide ideal substrates for detection of anti-HNE antibodies and peptide antioxidantsBiji T Kurien
Arthritis and Immunology Program, 825 NE 13th Street, Oklahoma City, OK 73104, USA
J Immunol Methods 303:66-75. 2005..Glycyl-proline dipeptide and a MAP from the Sm autoantigen (PPPGMRPP) showed 0% inhibition of HNE-modification... - Prolidase deficiency and the biochemical assays used in its diagnosisBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
Anal Biochem 349:165-75. 2006 - Barbering in mice: a model for trichotillomaniaBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
BMJ 331:1503-5. 2005 - Western blottingBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA
Methods 38:283-93. 2006..Finally, a brief description of methods generally used to detect antigens on blots is also described... 
An overview of Western blotting for determining antibody specificities for immunohistochemistryBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
Methods Mol Biol 717:55-67. 2011..Specificity of antibodies used for immunohistochemistry is of critical importance and therefore Western blot is a "must" to address antibodies' specificity...- Improving the solubility and pharmacological efficacy of curcumin by heat treatmentBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
Assay Drug Dev Technol 5:567-76. 2007..Heat-solubilized curcumin should be considered in clinical trials involving curcumin, especially in the face of frustrating results obtained regarding curcumin-mediated correction of cystic fibrosis defects... - Can low dose diagnostic dental radiation trigger Sjögren's syndrome?Biji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
Med Hypotheses 69:995-1000. 2007..We hypothesize that diagnostic dental radiation could be a factor for the development of SS as a result of either radiation induced apoptosis or through oxidative modification of proteins... - Curcumin/turmeric solubilized in sodium hydroxide inhibits HNE protein modification--an in vitro studyBiji T Kurien
Arthritis and Immunology Program, Oklahoma City, OK 73104, USA
J Ethnopharmacol 110:368-73. 2007..The pH at which the inhibition of HNE modification of substrate was observed was close to the physiological pH, making this formulation of curcumin potentially useful practically... - Autoantibody determination in the diagnosis of systemic lupus erythematosusB T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
Scand J Immunol 64:227-35. 2006..While the American College of Rheumatology classification criteria for SLE are primarily a tool for research, they may be useful clinically, in that those patients fulfilling four or more criteria are highly likely to have SLE... - Lipid peroxidation in systemic lupus erythematosusBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, USA
Indian J Exp Biol 44:349-56. 2006..Immunization with an oxidatively modified autoantigen led to the rapid development of autoimmunity compared to immunization with the unmodified autoantigen. Thus, oxidative damage appears to play an important role in SLE pathogenesis... - Selective small antigenic structures are capable of inducing widespread autoimmunity which closely mimics the humoral fine specificity of human SLEM T McClain
Oklahoma Research Foundation, Oklahoma City, OK 73114, USA
Scand J Immunol 56:399-407. 2002..These results demonstrate that loss of tolerance to select single, small antigenic structures can begin a cascade which virtually recreates, at the epitope level, the humoral autoimmune specificity seen in human SLE... - Immunization with peptides from 60 kDa Ro in diverse mouse strainsR H Scofield
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Department of Medicine, University of Oklahoma Health Sciences Center and the Department of Veterans Affairs Medical Center, Oklahoma City, OK 73105, USA
Scand J Immunol 56:477-83. 2002..Furthermore, these data suggest that genetic variation is important with regard to responding towards short peptide immunization by epitope spreading... - Induction of oral tolerance in experimental Sjogren's syndrome autoimmunityB T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
Scand J Immunol 61:418-25. 2005..Thus, epitope spreading was prevented, lymphocytic infiltration was blocked and saliva flow was restored by means of oral feeding of either Ro 274 or Ro 60 in this animal model of SS... - Autoantibody to the leucine zipper region of 52 kDa Ro/SSA binds native 60 kDa Ro/SSA: identification of a tertiary epitope with components from 60 kDa Ro/SSA and 52 kDa Ro/SSAB T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, 825 NE 13th Street, Oklahoma City, OK 73104, USA
Scand J Immunol 53:268-76. 2001..These data suggest that these two molecular species, 60 and 52 kDa Ro, form a conformational epitope. This relationship may explain why anti-52 kDa Ro is found in association with anti-60 kDa Ro... - Protein-protein interaction of the Ro-ribonucleoprotein particle using multiple antigenic peptidesR H Scofield
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
Mol Immunol 36:1093-106. 1999..The present data indicate that there are protein-protein interactions between Ro and La... - Strip immunoblotting of multiple antigenic peptides to nitrocellulose membraneBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, OKC, OK, 73104, USA
Methods Mol Biol 536:191-9. 2009..They lend themselves to analysis on an immunoblot as they behave like low molecular weight proteins. Affinity immunoblotting for analysis of antibody clonotype distribution has also been carried out using these peptides... - Interaction of calcium and Ro60: increase of antigenicityR Hal Scofield
Arthritis and Immunology Program, Oklahoma Medical Research Foundation (OMRF, 825 NE 13th Street, MS# 38, Oklahoma City, OK 73104, USA
Mol Immunol 41:809-16. 2004..Thus, the observed intramolecular and intermolecular interactions and antigen-antibody interactions could be Ca2+ ion mediated conformational interactions, and we propose that 60 kDa Ro is a calcium binding protein... - Association of neutropenia in systemic lupus erythematosus (SLE) with anti-Ro and binding of an immunologically cross-reactive neutrophil membrane antigenB T Kurien
Arthritis Immunology and Cardiovascular Biology Programs, Oklahoma Medical Research Foundation, and Department of Medicine, University of Oklahoma Health Science Center, Veterans Affairs Medical Center, Oklahoma City, OK, USA
Clin Exp Immunol 120:209-17. 2000..These data imply that anti-Ro antibodies that also bind the 64-kD protein mediate neutropenia in patients with SLE... - Detection of calcium binding by Ro 60 multiple antigenic peptides on nitrocellulose membrane using Quin-2-Biji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
Methods Mol Biol 536:483-90. 2009..These data, along with our observation regarding the involvement of calcium in the protein-protein interaction occurring between Ro 60 antigen and Ro 60 MAPs, make us propose that the Ro 60 antigen is a calcium binding protein... - Anti-calreticulin segregates anti-Ro sera in systemic lupus erythematosus: anti-calreticulin is present in sera with anti-Ro alone but not in anti-Ro sera with anti-La or anti-ribonucleoproteinR H Scofield
Department of Medicine, University of Oklahoma Health Sciences Center, The Veterans Affairs Medical Center, and the W K Warren Medical Research Institute, Oklahoma City, USA
J Rheumatol 27:128-34. 2000..To examine a well characterized group of patients with systemic lupus erythematosus (SLE) for anti-calreticulin... - Pellet pestle homogenization of agarose gel slices at 45 degrees C for deoxyribonucleic acid extractionB T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
Anal Biochem 296:162-6. 2001..Also, the number of transformants obtained using the DNA extracted with our method was at least twice that obtained using the DNA extracted with the commercial kit... - Affinity immunoblotting for analysis of antibody clonotype distribution in a lupus patient developing anti-Ro 60 over timeBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA
Methods Mol Biol 536:45-53. 2009..The bound IgG clonotypes were detected using alkaline phosphatase conjugated anti-IgG. The patient's sera demonstrated an oligoclonal response to the Ro 60 autoantigen that increased in complexity and affinity over time... - Ultrarapid electrophoretic transfer of high and low molecular weight proteins using heatBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, OKC, OK, 73104, USA
Methods Mol Biol 536:181-90. 2009..In summary, this procedure is particularly useful for the transfer of high molecular weight proteins, very rapid, and avoids the use of methanol... - Immunoblotting of multiple antigenic peptidesB T Kurien
Department of Arthritis and Immunology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
Electrophoresis 19:1659-61. 1998..High performance liquid chromatography purification of the MAPs is mainly responsible for their migration as sharp bands in SDS-PAGE and immunoblotting... - Oral manifestations of Sjögren's syndromeS A Mathews
University of Central Oklahoma, Edmond, OK, USA
J Dent Res 87:308-18. 2008..The most prevailing cause of xerostomia in elderly persons is the use of anticholinergic medications. Currently, there is no cure for Sjögren's syndrome, and treatment is mainly palliative... - Multiple immunoblots after non-electrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigensB T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
J Immunol Methods 205:91-4. 1997..In fact, we have used this procedure to obtain 12 immunoblots from a single gel with multiple sera... - Mouse urine collection using clear plastic wrapB T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
Lab Anim 33:83-6. 1999..Without coercion pure urine was obtained as early as 12 s. Volumes in the range of 10-250 microl were obtained. Modifications of the SAM could prove useful for rat or mouse urine collection under conditions of microgravity... - Can B cell epitopes of 60 kDa Ro distinguish systemic lupus erythematosus from Sjögren's syndrome?A N Scofield
Oklahoma Medical Research Program, University of Oklahoma Health Sciences Center, USA
Lupus 10:547-53. 2001..Thus, the fine specificity of antibody binding to 60 kDa Ro varies according to the presence of anti-La, not to the diagnosis of either SLE or SS... - Autoimmunity and oxidatively modified autoantigensBiji T Kurien
Arthritis and Immunology Program, USA
Autoimmun Rev 7:567-73. 2008..However, this should be viewed in the light of disappointing results obtained with the use of antioxidants in cardiovascular disease... - Nonelectrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens to obtain 12 immunoblotsBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
Methods Mol Biol 536:55-65. 2009.... - Affinity purification of autoantibodies from an antigen strip excised from a nitrocellulose protein blotBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, OKC, OK, 73104, USA
Methods Mol Biol 536:201-11. 2009..The autoantibodies purified in this manner reproduced the multiple nuclear dot anti-nuclear antibody pattern obtained using crude sera from 21 patients without primary biliary cirrhosis or anti-mitochondrial antibody... - A brief review of other notable protein blotting methodsBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
Methods Mol Biol 536:367-84. 2009..All these methods are briefly reviewed in this chapter... - A brief review of other notable protein detection methods on blotsBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA
Methods Mol Biol 536:557-71. 2009..These methods are briefly reviewed in this chapter... - Immunization with short peptides from the 60-kDa Ro antigen recapitulates the serological and pathological findings as well as the salivary gland dysfunction of Sjogren's syndromeR Hal Scofield
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
J Immunol 175:8409-14. 2005..BALB/c mice immunized with 60-kDa Ro peptides develop anti-Ro, salivary gland lymphocyte infiltrates, and salivary dysfunction that is highly reminiscent of human Sjögren's syndrome... - Awaken olfactory receptors of humans and experimental animals by coffee odourants to induce appetiteYaser Dorri
Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, OK 73104, United States
Med Hypotheses 69:508-9. 2007..Another hypothesis might be the unique structure of these coffee odourants. Studies need to be done to investigate the effect of coffee smell on salivary flow and appetite in animals and humans... - Cost-benefit analysis of a method using diatomaceous earth to purify Tamm-Horsfall proteinJaideep Shenoi
University of Oklahoma Health Sciences Center, Medicine-Pediatrics, 4502 Shusterman Center, Tulsa, OK 74135, USA
Biotechniques 36:64-6. 2004 - Modification of lupus-associated 60-kDa Ro protein with the lipid oxidation product 4-hydroxy-2-nonenal increases antigenicity and facilitates epitope spreadingR Hal Scofield
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
Free Radic Biol Med 38:719-28. 2005..These data may suggest a hitherto unappreciated mechanism by which oxidative stress facilitates epitope spreading in SLE... - Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membraneB T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
Indian J Biochem Biophys 38:274-6. 2001..This procedure enabled us to identify a cross-reactive D1 antigen on the neutrophil surface that bound antibodies that targeted 60 kD Ro autoantigen in systemic lupus erythematosus, an autoimmune disorder... - Tryptic peptide purification using polyvinylidene difluoride membrane for matrix-assisted laser desorption ionization time of flight mass spectrometryBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
Methods Mol Biol 536:39-44. 2009..Using this procedure, we were able to identify a cross-reactive D1 autoantigen on the surface of neutrophils that bound antibodies targeting Ro 60 autoantigen in systemic lupus erythematosus... - Renaturation of recombinant Ro 60 autoantigen by calcium ions on PVDF membraneBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
Methods Mol Biol 536:299-306. 2009..Since the immmunological epitopes of Ro 60 are mainly conformational, we believe that calcium induced a more native tertiary structure in recombinant Ro 60 autoantigen -following blotting to a PVDF membrane... - Free radical mediated peroxidative damage in systemic lupus erythematosusBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
Life Sci 73:1655-66. 2003..Heat inactivated anti-SOD autoantibodies were able to inhibit the activity of the enzyme. We propose that the inhibition of SOD by autoantibodies is, in part, responsible for the increased free radical damage seen in the disease... - Detection of catalase as a major protein target of the lipid peroxidation product 4-HNE and the lack of its genetic association as a risk factor in SLEAnil D'Souza
Department of Arthritis and Immunology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA
BMC Med Genet 9:62. 2008..Hence we investigated the effects of a genetic polymorphism of catalase on risk and severity of SLE in 48 pedigrees with African American ancestry... - Problems with multiple use of transfer buffer in protein electrophoretic transferYaser Dorri
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma, USA
J Biomol Tech 21:1-2. 2010..Here, we comment on this report, as we believe this method is not accurate and useful for protein analysis, and it can cause background binding as well as inaccurate protein analysis... - Heat mediated quick Coomassie blue protein staining and destaining of SDS-PAGE gelsB T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
Indian J Biochem Biophys 35:385-9. 1998..For a 0.8 mm gel, the staining time could be reduced to 1 min at 65 degrees C compared to 2 min at 60 degrees C and 5 min at 55 degrees C. The destaining time required was 8, 15 and 20 min at the respective temperatures... - Immunization of mice with human 60-kd Ro peptides results in epitope spreading if the peptides are highly homologous between human and mouseR H Scofield
Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, Department of Veterans Affairs Medical Center, and WK Warren Medical Research Institute, Oklahoma City 73104, USA
Arthritis Rheum 42:1017-24. 1999..The purpose of this study was to determine the ability of several different peptides from 60-kd Ro to induce expansion of the immune response to the Ro/La RNP particle... - Introduction to protein blottingBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA
Methods Mol Biol 536:9-22. 2009..The scientific community is now confronted with a variety of ways and means to carry out this transfer... - Neonatal lupus manifests as isolated neutropenia and mildly abnormal liver functionsSivalingam Kanagasegar
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA
J Rheumatol 29:187-91. 2002..Furthermore, although other factors may contribute, these data suggest that anti-60 kDa Ro is directly involved in the pathogenesis of neutropenia... - Systemic lupus erythematosus and C1q: A quantitative ELISA for determining C1q levels in serumSkyler P Dillon
Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA
Biotechnol J 4:1210-4. 2009..We report an average concentration of 113 +/- 40 microg/mL for C1q in non-SLE serum. The assay designed here will be useful in the high-throughput measurement of serum C1q in SLE cases... - Oxidatively modified autoantigens in autoimmune diseasesBiji T Kurien
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, OK 73104, USA
Free Radic Biol Med 41:549-56. 2006.... - Inhalation of environmental stressors & chronic inflammation: autoimmunity and neurodegenerationSandra E Gomez-Mejiba
Free Radical Biology and Aging Research Program, MS 21, 825 NE 13th Street, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, United States
Mutat Res 674:62-72. 2009.... - Identification of lanthionine synthase C-like protein-1 as a prominent glutathione binding protein expressed in the mammalian central nervous systemCharlotte H Y Chung
Oklahoma Medical Research Foundation, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73118, USA
Biochemistry 46:3262-9. 2007..These results indicate LanCL1 is a glutathione binding protein possibly significant to neurodegenerative disease... - Sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ECL membrane assaysBiji T Kurien
Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104
Methods Mol Biol 536:573-81. 2009..In addition, this method could be used to mark nitrocellulose membranes for orientation purposes using ECL detection system and thus can eliminate the use of autoradiography pens... - Environmentally safe removal/disposal of Coomassie Brilliant Blue from gel destain and used gel stainYaser Dorri
Department of Endocrinology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Anal Biochem 404:193-6. 2010..Thus, stain removal with Kimwipes helps in disposing CBB in an environmentally friendly manner and allows recycling of destaining solution... - SOD1 interacts directly with hemoglobin in vitroBiji T Kurien
Thromb Haemost 92:218-20. 2004 - Reply to 'pH paper trumps expensive kits in measuring acidity'Biji T Kurien
Nat Med 13:1275. 2007 - Four men in a space station - To say nothing of the cow! The quest for finding respite and work in the ultimate frontierBiji T Kurien
Med Hypotheses 68:1195-9. 2007..The anaerobic environment is particularly suitable for biogas generation and if successful they plan to get NASA to launch space vehicles to Mars using this natural fuel with the ISS as the staging area... - A nonsense mutation of PEPD in four Amish children with prolidase deficiencyHeng Wang
Das Deutsch Center DDC Clinic for Special Needs Children, Middlefield, Ohio 44062, USA
Am J Med Genet A 140:580-5. 2006..793 T > C in exon 11, resulting in a premature stop-codon at amino acid residue 265 (p.R265X). It is speculated that the severe phenotype in these patients might be associated with the type of the PEPD gene mutation...